CN219156889U - Stem cell culture equipment - Google Patents

Stem cell culture equipment Download PDF

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Publication number
CN219156889U
CN219156889U CN202320125116.2U CN202320125116U CN219156889U CN 219156889 U CN219156889 U CN 219156889U CN 202320125116 U CN202320125116 U CN 202320125116U CN 219156889 U CN219156889 U CN 219156889U
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China
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heat preservation
stem cell
sealing ring
cell culture
inner door
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CN202320125116.2U
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Chinese (zh)
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王威
张俊
于佃才
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Sichuan Lejia Biotechnology Co ltd
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Sichuan Lejia Biotechnology Co ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The utility model discloses stem cell culture equipment, which comprises a culture outer box, a heat preservation culture mechanism and a split type airtight protection mechanism, wherein the heat preservation culture mechanism is arranged in the culture outer box and comprises a heat preservation inner container, a plurality of groups of evenly distributed object carrying plates are arranged in the heat preservation inner container, the split type airtight protection mechanism is arranged on one side of the heat preservation inner container and comprises a glass inner door, the glass inner door is matched with the heat preservation inner container, a plurality of evenly distributed ladder object taking holes are cut on the glass inner door and are matched with the object carrying plates, and independent observation doors are hinged in the ladder object taking holes. According to the utility model, through the arrangement of the corresponding mechanism, a single carrying tray can be independently observed and opened for taking, so that the heat loss of the carbon dioxide incubator in the process of opening the glass inner door is reduced, and the effect of the carbon dioxide incubator on culturing stem cells is improved.

Description

Stem cell culture equipment
Technical Field
The utility model belongs to the technical field of stem cell culture, and particularly relates to stem cell culture equipment.
Background
Stem cells are a class of cells with unlimited or immortalized self-renewal capacity capable of producing at least one type of highly differentiated daughter cells, and also capable of producing specialized cells that make up tissues and organs of the body, and also capable of differentiating into progenitor cells, which can be divided into: totipotent stem cells, pluripotent stem cells, monoenergetic stem cells and the like, and the culture of stem cells has great significance in the field of medical science and biological research.
The stem cell culture equipment is used for culturing stem cells, and the common stem cell culture equipment mainly comprises a carbon dioxide incubator, a constant temperature and humidity box, a high-speed centrifuge, a thermoelectric enzyme-labeling instrument, a PCR instrument and the like, wherein the carbon dioxide incubator has the advantages of easy cleaning, small temperature fluctuation and the like, and is widely applied.
The common carbon dioxide incubator in the general market mainly comprises an incubator, a heat preservation inner container, a carrying disc, a humidifying disc, a glass inner door, a temperature detection sensor, a control system and the like, and in the process of culturing stem cells, the heat preservation inner container can be subjected to auxiliary sealing heat preservation through the arrangement of the glass inner door, and an operator can observe the cell culture process in the heat preservation inner container conveniently.
However, most integration of glass internal door of current carbon dioxide incubator sets up, is opening the in-process to glass internal door, and a plurality of year thing dish can expose in the air simultaneously, not only can accelerate the loss of carbon dioxide incubator internal heat like this, makes carbon dioxide incubator easily polluted simultaneously, has reduced carbon dioxide incubator's stem cell culture effect.
Accordingly, in view of the above-described technical problems, it is necessary to provide a stem cell culture apparatus.
Disclosure of Invention
The utility model aims to provide stem cell culture equipment for solving the problem that the heat preservation and protection effects of the glass inner door in the carbon dioxide incubator are poor.
In order to achieve the above object, an embodiment of the present utility model provides the following technical solution:
a stem cell culture apparatus comprising: the device comprises a culture outer box, a heat preservation culture mechanism and a split type airtight protection mechanism;
the heat-preservation culture mechanism is arranged in the culture outer box and comprises a heat-preservation inner container, and a plurality of groups of evenly distributed object carrying discs are arranged in the heat-preservation inner container;
the split type airtight protection mechanism is arranged on one side of the heat preservation inner container, the split type airtight protection mechanism comprises a glass inner door, the glass inner door is matched with the heat preservation inner container, a plurality of evenly distributed ladder object taking holes are cut on the glass inner door, the ladder object taking holes are matched with the object carrying disc, the ladder object taking holes are hinged with independent observation doors, and one sides of the independent observation doors, which are located in the ladder object taking holes, are connected with observation limiting blocks.
Further, the heat preservation inner bag is located one side outside the incubator and is connected with fixed sealing washer, plays the effect of fixed spacing of absorption to the glass inner door through the mutually supporting of fixed sealing washer and sealing ring, has improved the leakproofness of glass inner door and heat preservation inner bag simultaneously through the mutually supporting of fixed sealing washer and sealing ring, has reduced the incubator at the heat dissipation in the heat preservation inner bag of opening the in-process, one side of fixed sealing washer closing to the glass inner door is excavated there is spacing draw-in groove, is convenient for carry out the block spacing to the sealing ring through the spacing draw-in groove of excavation.
Further, one side that fixed sealing washer was pressed close to the glass internal door is connected with the sealing ring, sealing ring and spacing draw-in groove assorted play fixed spacing and auxiliary sealing's effect to the glass internal door through the mutual block of sealing ring and fixed sealing washer, one side that the glass internal door kept away from the sealing ring is connected with and opens and close the handle, is convenient for carry out the switching control to the glass internal door through the mode of applying effort to opening and close the handle.
Further, a plurality of independent observation door and observation stopper are insulating glass heat preservation apron, just independent observation door and observation stopper integrated into one piece, through selecting for use insulating glass apron as independent observation door and observation stopper be convenient for observe carrying the thing dish, improved the convenience of observing stem cell culture dish on the heat preservation inner bag.
Further, a plurality of the outside of observing the stopper all overlaps and is equipped with spacing sealing ring, spacing sealing ring and ladder get thing hole assorted, through the outside cover of observing the stopper set up spacing sealing ring, be convenient for fix spacing to observing the stopper through spacing sealing ring and the mutual extruded mode of the lateral wall of ladder getting the thing hole, simultaneously, improved through spacing sealing ring and carried out closed seal to observing the stopper.
Further, a plurality of independent observation doors are all connected with the control piece in one side that keeps away from the observation stopper, are convenient for open and close control to independent observation door through the mode of applying effort to the control piece.
Compared with the prior art, the utility model has the following advantages:
according to the utility model, through the arrangement of the corresponding mechanism, a single carrying tray can be independently observed and opened for taking, so that the heat loss of the carbon dioxide incubator in the process of opening the glass inner door is reduced, and the effect of the carbon dioxide incubator on culturing stem cells is improved.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present utility model, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic diagram showing an open state of a stem cell culture apparatus according to an embodiment of the present utility model;
FIG. 2 is a schematic view showing a closed state of an inner glass door according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram showing an open state of an independent observation door according to an embodiment of the utility model;
FIG. 4 is a schematic diagram showing a part of a stem cell culturing apparatus according to an embodiment of the present utility model;
fig. 5 is a schematic diagram of the structure at a in fig. 4.
In the figure: 1. the device comprises a culture outer box, a heat preservation culture mechanism, a heat preservation inner container, a material carrying disc, a split type airtight protection mechanism, a glass inner door and a glass inner door, wherein the glass inner door is arranged in the culture outer box, the heat preservation culture mechanism is arranged in the culture outer box, the heat preservation inner container is arranged in the culture outer box, the material carrying disc is arranged in the culture outer box, the split type airtight protection mechanism is arranged in the culture outer box, the glass inner door is arranged in the culture outer box, the independent observation door is arranged in the culture outer box, the culture outer box is arranged in the insulation outer.
Detailed Description
The present utility model will be described in detail below with reference to the embodiments shown in the drawings. The embodiments are not intended to limit the utility model, but structural, methodological or functional modifications from the embodiments are within the scope of the utility model.
The utility model discloses stem cell culture equipment, which is shown in fig. 1-5 and comprises: the device comprises an outer incubator 1, a heat preservation culture mechanism 2 and a split type airtight protection mechanism 3.
Referring to fig. 1, a thermal insulation culture mechanism 2 is disposed in an incubator 1, and plays a role in thermal insulation storage on stem cell culture dishes through the thermal insulation culture mechanism 2, wherein the thermal insulation culture mechanism 2 comprises a thermal insulation liner 201, and plays a role in supporting, limiting and thermal insulation control on a plurality of groups of object carrying plates 202 through the thermal insulation liner 201.
Referring to fig. 1, a plurality of groups of evenly distributed carrier trays 202 are arranged in a heat preservation liner 201, and stem cell culture dishes are supported and stored through the plurality of groups of carrier trays 202.
Referring to fig. 1, the split type airtight protection mechanism 3 is disposed on one side of the heat preservation liner 201, and plays a role in airtight protection on the heat preservation liner 201 through the split type airtight protection mechanism 3, and meanwhile, it is convenient to observe the stem cell culture dish stored in the heat preservation liner 201.
Referring to fig. 1, the split type airtight protection mechanism 3 includes a glass inner door 301, the glass inner door 301 is matched with the heat insulation inner container 201, and the glass inner door 301 plays a role in supporting and limiting a plurality of independent observation doors 302, and meanwhile, the glass inner door 301 is convenient for carrying out closed heat insulation protection on the heat insulation inner container 201.
Wherein, a plurality of evenly distributed's ladder is got the thing hole on the glass inner door 301, and a plurality of ladder get thing hole and year thing dish 202 assorted, be convenient for independently take the stem cell culture dish that bears on a plurality of year thing dishes 202 through ladder get thing hole, reduced glass inner door 301 in opening the in-process, the circumstances that heat is lost in a large number of in the heat preservation inner bag 201.
Referring to fig. 1-2, the independent observation doors 302 are hinged in the plurality of ladder fetching holes, so that the ladder fetching holes are conveniently sealed and protected through the plurality of independent observation doors 302.
Referring to fig. 3-5, the sides of the plurality of independent observation doors 302 located in the ladder object taking holes are all connected with an observation limiting block 303, the independent observation doors 302 are limited and fixed in a manner of clamping and limiting the observation limiting block 303, meanwhile, the thickness of the independent observation doors 302 is increased through the observation limiting block 303, and the effect of heat preservation of the heat preservation liner 201 is improved.
Referring to fig. 3-5, a fixed sealing ring 304 is connected to one side of the heat insulation inner container 201 located outside the incubator 1, the glass inner door 301 is adsorbed and fixed and limited by the mutual cooperation of the fixed sealing ring 304 and the sealing ring 305, and meanwhile, the tightness of the glass inner door 301 and the heat insulation inner container 201 is improved by the mutual cooperation of the fixed sealing ring 304 and the sealing ring 305, so that heat dissipation in the heat insulation inner container 201 in the opening process of the incubator 1 is reduced.
Specifically, a limiting clamping groove is cut on one side of the fixed sealing ring 304, which is close to the glass inner door 301, and clamping and limiting of the sealing ring 305 are facilitated through cutting the limiting clamping groove.
Referring to fig. 3-5, a sealing ring 305 is connected to a side of the glass inner door 301 close to the fixed sealing ring 304, the sealing ring 305 is matched with the limiting clamping groove, and the fixing limiting and auxiliary sealing functions are achieved on the glass inner door 301 through mutual clamping of the sealing ring 305 and the fixed sealing ring 304.
Referring to fig. 1-2, an opening and closing handle 306 is connected to a side of the glass inner door 301 away from the sealing ring 305, so that the opening and closing of the glass inner door 301 can be controlled by applying a force to the opening and closing handle 306.
Specifically, a plurality of independent observation doors 302 and observe stopper 303 are insulating glass heat preservation apron, and independent observation doors 302 and observe stopper 303 integrated into one piece, are convenient for observe carrying tray 202 through selecting insulating glass apron as independent observation doors 302 and observe stopper 303, have improved the convenience of observing the stem cell culture dish on the heat preservation inner bag 201.
Referring to fig. 3-5, the outer sides of the plurality of observing limiting blocks 303 are respectively sleeved with a limiting sealing ring 307, the limiting sealing rings 307 are matched with the ladder object taking holes, the limiting sealing rings 307 are sleeved on the outer sides of the observing limiting blocks 303, the observing limiting blocks 303 are fixed and limited in a manner that the limiting sealing rings 307 and the side walls of the ladder object taking holes are mutually extruded, and meanwhile, the sealing performance of the observing limiting blocks 303 is improved through the limiting sealing rings 307.
Referring to fig. 2, a control member 308 is connected to a side of each of the plurality of independent observation doors 302, which is far away from the observation limiting block 303, so that the independent observation doors 302 can be conveniently opened or closed by applying a force to the control member 308.
When the device is specifically used, the heat preservation liner 201 is sealed and limited through the mutual coordination of the glass inner door 301, the plurality of independent observation doors 302 and the observation limiting blocks 303, and meanwhile, the glass inner door 301 is fixed and limited and sealed heat preservation is controlled through the mutual coordination of the fixed sealing rings 304 and the sealing rings 305, so that the condition that heat loss occurs when a stem cell culture dish in the heat preservation liner 201 is observed is reduced;
when the stem cell culture rice stored on the carrying tray 202 needs to be taken, an operator can open the single independent observation door 302 in a mode of drawing the control piece 308, and then can take the stem cell culture dish stored on the carrying tray 202, heat loss in the heat preservation liner 201 is reduced in a mode of opening the single independent observation door 302, and the heat preservation culture effect of the heat preservation liner 201 is improved.
The technical scheme shows that the utility model has the following beneficial effects:
according to the utility model, through the arrangement of the corresponding mechanism, a single carrying tray can be independently observed and opened for taking, so that the heat loss of the carbon dioxide incubator in the process of opening the glass inner door is reduced, and the effect of the carbon dioxide incubator on culturing stem cells is improved.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment contains only one independent technical solution, and that such description is provided for clarity only, and that the technical solutions of the embodiments may be appropriately combined to form other embodiments that will be understood by those skilled in the art.

Claims (6)

1. A stem cell culture apparatus, comprising:
a culture outer box (1);
the heat preservation culture mechanism (2) is arranged in the culture outer box (1), the heat preservation culture mechanism (2) comprises a heat preservation inner container (201), and a plurality of groups of evenly distributed carrying trays (202) are arranged in the heat preservation inner container (201);
split type airtight protection mechanism (3) locates one side of heat preservation inner bag (201), split type airtight protection mechanism (3) are including glass inner door (301), glass inner door (301) and heat preservation inner bag (201) assorted, it has a plurality of evenly distributed's ladder to get the thing hole to open on glass inner door (301), and is a plurality of ladder is got the thing hole and is carried thing dish (202) assorted, and is a plurality of the ladder is got downthehole all to articulate has independent observation door (302), a plurality of independent observation door (302) are located one side that the ladder got the thing downthehole all is connected with and observe stopper (303).
2. The stem cell culture apparatus according to claim 1, wherein a fixed sealing ring (304) is connected to one side of the heat preservation inner container (201) located outside the culture outer box (1), and a limit clamping groove is cut on one side of the fixed sealing ring (304) close to the glass inner door (301).
3. The stem cell culture apparatus according to claim 2, wherein a sealing ring (305) is connected to the glass inner door (301) at a side close to the fixed sealing ring (304), the sealing ring (305) is matched with the limit clamping groove, and an opening and closing handle (306) is connected to the glass inner door (301) at a side far away from the sealing ring (305).
4. The stem cell culture apparatus according to claim 1, wherein the independent observation doors (302) and the observation limiting blocks (303) are all insulating glass insulating cover plates, and the independent observation doors (302) and the observation limiting blocks (303) are integrally formed.
5. The stem cell culture apparatus according to claim 1, wherein a plurality of the observation limiting blocks (303) are respectively provided with a limiting sealing ring (307) in a sleeved mode, and the limiting sealing rings (307) are matched with the stepped object taking holes.
6. A stem cell culture apparatus according to claim 1, wherein a control member (308) is connected to a side of the plurality of independent observation doors (302) remote from the observation stopper (303).
CN202320125116.2U 2023-02-06 2023-02-06 Stem cell culture equipment Active CN219156889U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202320125116.2U CN219156889U (en) 2023-02-06 2023-02-06 Stem cell culture equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202320125116.2U CN219156889U (en) 2023-02-06 2023-02-06 Stem cell culture equipment

Publications (1)

Publication Number Publication Date
CN219156889U true CN219156889U (en) 2023-06-09

Family

ID=86643857

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202320125116.2U Active CN219156889U (en) 2023-02-06 2023-02-06 Stem cell culture equipment

Country Status (1)

Country Link
CN (1) CN219156889U (en)

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