CN219136813U

CN219136813U - Culture bottle for three-dimensional cell culture

Info

Publication number: CN219136813U
Application number: CN202123213790.6U
Authority: CN
Inventors: 赵钢; 李子洋
Original assignee: Suzhou Novi Bio Technology Co ltd; Tianjin Norway Baiao Technology Co ltd
Current assignee: Suzhou Novi Bio Technology Co ltd; Tianjin Norway Baiao Technology Co ltd
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2023-06-06
Anticipated expiration: 2031-12-20

Abstract

A culture bottle for three-dimensional cell culture comprises a bottle body and a bottle cap, wherein the bottle body and the bottle cap are detachably connected, a plurality of cell culture devices for cell attachment growth are arranged at the bottom of the bottle body, and a ventilation device is arranged on the bottle body and the bottle cap. According to the utility model, the column body treated by the TC treatment process is arranged at the bottom of the culture bottle, cells can longitudinally grow attached to the column body, and the whole culture area is enlarged in the same use area; the hydrophobic membrane is added at the bottle body and the bottle cap, so that the replacement amount of gas can be increased, the gas proportion in the bottle can be better maintained, the large-scale and high-density cell culture is facilitated, the process is simplified, and the cost and the culture occupied area are saved. The device simple structure, convenient operation, flexible operation, it is little to cultivate area, and the practicality is strong.

Description

Culture bottle for three-dimensional cell culture

Technical Field

The utility model relates to the technical field of cell culture flasks, in particular to a culture flask for three-dimensional culture of cells.

Background

At present, cells, especially stem cells, tumor cells and the like need to be cultivated in an adherent way, so that a large amount of area is needed for cultivation, and a large number of single-sided culture flasks are used in the cultivation process.

Disclosure of Invention

The object of the present utility model is to solve at least one of the technical drawbacks.

Therefore, the utility model aims to provide a culture bottle for three-dimensional cell culture, which solves the problems in the background art and overcomes the defects in the prior art.

A blake bottle for three-dimensional culture of cell, includes bottle and bottle lid, bottle and bottle lid detachably connect, the bottom of bottle is equipped with a plurality of cell culture devices that are used for cell attached growth, the bottle with be equipped with ventilation unit on the bottle lid.

Preferably, the cell culture device is a plurality of columns arranged at the bottom of the bottle body, and the surfaces of the columns are treated by a TC treatment process.

It is preferable in any of the above-mentioned cases that the distance between the columns is 0.34 to 0.36cm.

In any of the above embodiments, it is preferable that the ventilation means is provided on a side wall of an upper portion of the bottle body and a top of the bottle cap.

In any of the above aspects, preferably, the ventilation means is a hydrophobic membrane.

In any of the above embodiments, it is preferable that the material of the hydrophobic membrane is polytetrafluoroethylene.

In any of the above embodiments, the pore diameter of the hydrophobic film is preferably 0.2. Mu.m.

In any of the above embodiments, it is preferable that the width of the upper portion of the bottle body is smaller than the width of the lower portion of the bottle body.

By any of the above schemes, preferably, the top of the bottle body is provided with a bottle opening, and the bottle opening is connected with the bottle cap through threads.

In any of the above embodiments, it is preferable that the material of the bottle body and the bottle cap is polystyrene.

Compared with the prior art, the utility model has the following advantages and beneficial effects:

according to the utility model, the column body treated by the TC treatment process is arranged at the bottom of the culture bottle, cells can longitudinally grow attached to the column body, and the whole culture area is enlarged in the same use area;

the hydrophobic membrane is added at the bottle body and the bottle cap, so that the replacement amount of gas can be increased, the gas proportion in the bottle can be better maintained, the large-scale and high-density cell culture is facilitated, the process is simplified, and the cost and the culture occupied area are saved;

in the culture process of the culture bottle, the culture liquid needs to form a vortex shape, a structure with a narrow upper part and a wide lower part of the bottle body is arranged to form a barrier, so that the culture medium at the lower part of the bottle body is prevented from splashing to the upper part of the bottle body, especially the bottle cap and the upper hydrophobic membrane, in the rotation process of the culture medium at the lower part of the bottle body, and the culture medium is prevented from being polluted by the culture medium to cause abnormality.

The utility model has the advantages of simple structure, convenient operation, flexible operation, small culture occupied area and strong practicability.

Drawings

The foregoing and/or additional aspects and advantages of the utility model will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 is a schematic view of the structure of a bottle body of the present utility model;

FIG. 2 is a top view of the bottle;

FIG. 3 is a top view of the A-A' side of the bottle;

FIG. 4 is a schematic view of the structure of the screw cap of the present utility model;

FIG. 5 is a top view of the screw cap of the present utility model;

fig. 6 is a view showing the structure of the combined bottle body and cap.

Wherein:

1. a bottle mouth; 2. a thread; 3. a bottle body; 4. a hydrophobic membrane; 5. a column;

6. the upper part of the bottle body; 7. the bottom of the bottle body; 8. a bottle cap; 9. the lower part of the bottle body.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

The culture bottle for three-dimensional cell culture comprises a bottle body 3 and a bottle cap 8, wherein the bottle body 3 and the bottle cap 8 are detachably connected, a plurality of cell culture devices for cell attachment growth are arranged at the bottom of the bottle body 3, and a ventilation device is arranged on the bottle body 3 and the bottle cap 8.

The top of the bottle body 3 is provided with a bottle opening 1, the bottle opening 1 and the bottle cap 8 are connected through threads 2, and the bottle body 3 and the bottle cap 8 are made of polystyrene.

The structure of the bottle body 3 shown in fig. 1 is that the width of the upper part 6 of the bottle body is smaller than the width of the lower part 9 of the bottle body, namely, the upper part is narrow, the lower part is wide, the design forms an obstacle, and in the culture process of the culture bottle, the culture medium of the lower part 9 of the bottle body is prevented from splashing to the upper part 6 of the bottle body, particularly, the hydrophobic membrane 4 at the bottle cap 8 and the hydrophobic membrane 4 at the upper part 6 of the bottle body in the rotation process of the culture medium, so that the culture medium is polluted by the culture medium and the culture medium is abnormal.

The cell culture apparatus shown in FIG. 3 is a plurality of columns 5 arranged at the bottom 7 of the bottle body, and the surface of the columns 5 is treated by TC treatment process; the distance between the columns 5 is 0.34-0.36cm, preferably the distance between the columns 5 is 0.35cm.

The ventilation means are provided on the side wall of the upper part 6 of the bottle body and on the top of the cap 8 as shown in figures 1, 4 and 5; the ventilation device is a hydrophobic membrane 4, the material of the hydrophobic membrane 4 is polytetrafluoroethylene, and the pore diameter of the hydrophobic membrane 4 is 0.2 mu m.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

It will be readily understood by those skilled in the art that the present utility model, including any combination of parts described in the summary and detailed description of the utility model above and shown in the drawings, is limited in scope and does not constitute a complete description of the various aspects of these combinations for the sake of brevity. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Although embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives, and variations may be made in the above embodiments by those skilled in the art without departing from the spirit and principles of the utility model. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A culture flask for three-dimensional cell culture, which is characterized in that: the cell culture device comprises a bottle body and a bottle cap, wherein the bottle body and the bottle cap are detachably connected, a plurality of cell culture devices for cell attachment growth are arranged at the bottom of the bottle body, and ventilation devices are arranged on the bottle body and the bottle cap; the cell culture device is a plurality of cylinders arranged at the bottom of the bottle body.

2. A flask for three-dimensional culture of cells according to claim 1, wherein: the spacing distance between the columns is 0.34-0.36cm.

3. A flask for three-dimensional culture of cells according to claim 1, wherein: the ventilation device is arranged on the side wall of the upper part of the bottle body and the top of the bottle cap.

4. A flask for three-dimensional culture of cells according to claim 3, wherein: the ventilation device is a hydrophobic film.

5. The flask for three-dimensional culture of cells of claim 4, wherein: the material of the hydrophobic membrane is polytetrafluoroethylene.

6. The flask for three-dimensional culture of cells of claim 5, wherein: the pore size of the hydrophobic membrane is 0.2 μm.

7. A flask for three-dimensional culture of cells according to any one of claims 1-6, wherein: the width of bottle upper portion is less than the width of bottle lower part.

8. The flask for three-dimensional culture of cells of claim 7, wherein: the top of the bottle body is provided with a bottle opening, and the bottle opening is connected with the bottle cap through threads.

9. The flask for three-dimensional culture of cells of claim 8, wherein: the bottle body and the bottle cap are made of polystyrene.