CN219117462U - Stem cell hypoxia culture device - Google Patents
Stem cell hypoxia culture device Download PDFInfo
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- CN219117462U CN219117462U CN202223049074.3U CN202223049074U CN219117462U CN 219117462 U CN219117462 U CN 219117462U CN 202223049074 U CN202223049074 U CN 202223049074U CN 219117462 U CN219117462 U CN 219117462U
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Abstract
The utility model discloses a stem cell hypoxia culture device, which belongs to the technical field of stem cell hypoxia culture, and adopts the technical scheme that the stem cell hypoxia culture device comprises a stem cell hypoxia culture device body, a culture mechanism and an oxygen supply mechanism, wherein the culture mechanism is connected to the inner wall of the stem cell hypoxia culture device body in a sliding way, the oxygen supply mechanism is bolted to the rear side of the stem cell hypoxia culture device body, a base component in the culture mechanism can store a containing component and can provide oxygen for the containing component, the containing component can always keep a hypoxia environment, the containing component can store and culture stem cells, the containing component can be used for culturing stem cells of at most four different types, the containing component can be taken off and replaced from the base component, and the oxygen supply mechanism can be externally connected with hypoxia supply equipment, so that hypoxia oxygen is provided for the culture mechanism, and the quality of the survival environment of the stem cells in the culture mechanism is maintained.
Description
Technical Field
The utility model relates to the technical field of stem cell hypoxia culture, in particular to a stem cell hypoxia culture device.
Background
Stem cells are a class of cells with unlimited or immortalized self-renewing capacity that are capable of producing at least one type of highly differentiated daughter cells, and require less oxygen than air, and thus require cultivation in a hypoxic culture apparatus.
When the stem cells are required to be cultured, a low-oxygen culture device is used, but when the existing stem cells low-oxygen culture device is used, a plurality of cells cannot be cultured in the same culture device, so that when the cell culture state is observed, the culture device is required to be frequently opened, oxygen in the air enters the culture device, the environmental quality of stem cell culture is reduced, and when different types of stem cells are replaced, the whole culture device is required to be replaced, and the replacement cost of the culture device is increased.
Disclosure of Invention
The utility model provides a stem cell hypoxia culture device, which aims to solve the problems that when the conventional stem cell hypoxia culture device is used, a plurality of cells cannot be cultured in the same culture device, so that when a cell culture state is observed, the culture device is required to be frequently opened, oxygen in air enters the culture device, the environmental quality of stem cell culture is reduced, and when different types of stem cells are replaced, the whole culture device is required to be replaced, and the replacement cost of the culture device is increased.
The utility model is realized in such a way that the stem cell hypoxia culture device comprises a stem cell hypoxia culture device body, a culture mechanism and an oxygen supply mechanism, wherein the culture mechanism is connected to the inner wall of the stem cell hypoxia culture device body in a sliding way, the oxygen supply mechanism is bolted to the rear side of the stem cell hypoxia culture device body, and the rear side of the culture mechanism is communicated with the oxygen supply mechanism;
the culture mechanism comprises a base component and an accommodating component, wherein the base component comprises a bearing plate, a placing groove and an oxygen supply groove, the bearing plate is slidably connected with the inner wall of the stem cell hypoxia incubator body, the placing groove is formed in the top of the bearing plate, the oxygen supply groove is formed in the rear side of the inner wall of the placing groove, the accommodating component comprises a culture dish body, a sliding plate cover and an air inlet hole, the culture dish body is clamped on the inner wall of the placing groove, the sliding plate cover is slidably connected with the top of the culture dish body, and the air inlet hole is formed in the rear side of the culture dish body.
In order to facilitate the effect of oxygen supply to the culture mechanism, as a stem cell hypoxia culture device of the utility model, preferably, the oxygen supply mechanism comprises a storage shell, a connecting shell and an oxygen pipe, wherein the storage shell is bolted on the inner wall of the stem cell hypoxia culture device body, the inner wall of the storage shell is in sliding connection with a bearing plate, the connecting shell is bolted on the rear side of the stem cell hypoxia culture device body, the oxygen pipe is communicated with the connecting shell, the oxygen pipe penetrates through the connecting shell and is communicated with the storage shell, and one side of the oxygen pipe close to an oxygen supply groove is communicated with the oxygen supply groove.
In order to facilitate distinguishing the effect of the direction of the culture dish body, as the stem cell hypoxia culture device provided by the utility model, preferably, positioning grooves are formed in both sides and the front side of the inner wall of the placing groove, positioning blocks are bolted to both sides and the front side of the culture dish body, the positioning blocks are clamped with the positioning grooves, the positioning blocks are matched with the culture dish body, and the positioning grooves are matched with the placing groove.
In order to increase the stability effect of the carrier plate during movement, as the stem cell hypoxia culture device provided by the utility model, preferably, the two sides of the carrier plate are provided with the stabilizing grooves, the two sides of the inner wall of the storage shell are respectively bolted with the stabilizing blocks, the stabilizing grooves are in sliding connection with the stabilizing blocks, the stabilizing blocks are matched with the storage shell for use, and the stabilizing grooves are matched with the carrier plate for use.
In order to increase the effect of the connection tightness of the oxygen therapy tube and the oxygen supply groove, the stem cell hypoxia culture device is preferably adopted, a sealing ring is clamped on one side of the oxygen therapy tube, which is close to the oxygen supply groove, the surface of the sealing ring is carved with anti-skid lines, and the sealing ring is matched with the oxygen therapy tube for use.
In order to facilitate the sliding effect of the slide plate cover, the stem cell hypoxia culture device is preferably characterized in that the top of the slide plate cover is provided with a storage groove, two sides of the inner wall of the storage groove are rotatably connected with a poking plate, and the poking plate is matched with the slide plate cover for use.
In order to increase the sealing effect of the sliding plate cover and the culture dish body when being closed, the sealing gasket is clamped at the front side of the sliding plate cover, the sealing gasket is made of rubber materials, anti-skid lines are carved on the surface of the sealing gasket, and the sealing gasket is matched with the sliding plate cover for use.
In order to increase the effect of the sliding stability of the sliding plate cover on the culture dish body, as the stem cell hypoxia culture device of the utility model, preferably, sliding grooves are formed in two sides of the inner wall of the culture dish body, sliding plates are bolted to two sides of the sliding plate cover, the sliding grooves are in sliding connection with the sliding plates, the sliding grooves are matched with the culture dish body, and the sliding plates are matched with the sliding plate cover.
Compared with the prior art, the utility model has the beneficial effects that:
this low oxygen culture apparatus of stem cell through setting up culture mechanism and oxygen suppliment mechanism, the base subassembly in the culture mechanism can be stored to can provide oxygen to holding the subassembly, let hold the subassembly in keeping low oxygen environment all the time, hold the subassembly and can store the culture with the stem cell, and hold the subassembly and can cultivate for four kinds at most different kinds of stem cell, hold the subassembly and can take off and change from the base subassembly, oxygen suppliment mechanism can external low oxygen supply equipment, thereby provide low oxygen to culture mechanism, keep the quality of stem cell living environment in the culture mechanism.
Drawings
FIG. 1 is a diagram showing the overall structure of a stem cell hypoxia culture device of the present utility model;
FIG. 2 is a block diagram of a base assembly of the present utility model;
FIG. 3 is a block diagram of a containment assembly of the present utility model;
FIG. 4 is a block diagram of the oxygen supply mechanism of the present utility model;
fig. 5 is a structural view of a sliding plate in the present utility model.
In the figure, 1, a stem cell hypoxia incubator body; 2. a culturing mechanism; 201. a base assembly; 2011. a carrying plate; 2012. a placement groove; 2013. an oxygen supply tank; 202. a housing assembly; 2021. a culture dish body; 2022. a slide plate cover; 2023. an air inlet hole; 3. an oxygen supply mechanism; 301. a storage case; 302. a connection housing; 303. an oxygen therapy tube; 4. a positioning groove; 5. a positioning block; 6. a stabilizing groove; 7. a stabilizing block; 8. a seal ring; 9. a storage groove; 10. a poking plate; 11. a sealing gasket; 12. a sliding groove; 13. and a sliding plate.
Detailed Description
The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model. Furthermore, in the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1-5, the present utility model provides the following technical solutions: the stem cell hypoxia culture device comprises a stem cell hypoxia culture device body 1, a culture mechanism 2 and an oxygen supply mechanism 3, wherein the culture mechanism 2 is connected to the inner wall of the stem cell hypoxia culture device body 1 in a sliding manner, the oxygen supply mechanism 3 is bolted to the rear side of the stem cell hypoxia culture device body 1, and the rear side of the culture mechanism 2 is communicated with the oxygen supply mechanism 3;
the culture mechanism 2 comprises a base assembly 201 and an accommodating assembly 202, the base assembly 201 comprises a bearing plate 2011, a placing groove 2012 and an oxygen supply groove 2013, the bearing plate 2011 is slidably connected to the inner wall of the stem cell hypoxia incubator body 1, the placing groove 2012 is formed in the top of the bearing plate 2011, the oxygen supply groove 2013 is formed in the rear side of the inner wall of the placing groove 2012, the accommodating assembly 202 comprises a culture dish body 2021, a sliding plate cover 2022 and an air inlet hole 2023, the culture dish body 2021 is connected to the inner wall of the placing groove 2012 in a clamping mode, the sliding plate cover 2022 is slidably connected to the top of the culture dish body 2021, and the air inlet hole 2023 is formed in the rear side of the culture dish body 2021.
In this embodiment: through setting up culture mechanism 2 and oxygen suppliment mechanism 3, the base subassembly 201 in the culture mechanism 2 can be stored to can provide oxygen to hold subassembly 202, let hold the subassembly 202 in remain the hypoxic environment all the time, hold subassembly 202 can store the stem cell and cultivate, and hold subassembly 202 can cultivate for four kinds at most different types stem cell, hold subassembly 202 can be taken off and change from base subassembly 201, oxygen suppliment mechanism 3 can external hypoxia supply equipment, thereby provide hypoxic oxygen to culture mechanism 2, keep the quality of the stem cell living environment in the culture mechanism 2.
As a technical optimization scheme of the utility model, the oxygen supply mechanism 3 comprises a storage shell 301, a connection shell 302 and an oxygen pipe 303, wherein the storage shell 301 is bolted on the inner wall of the stem cell hypoxia incubator body 1, the inner wall of the storage shell 301 is in sliding connection with a bearing plate 2011, the connection shell 302 is bolted on the rear side of the stem cell hypoxia incubator body 1, the oxygen pipe 303 is communicated with the connection shell 302, the oxygen pipe 303 penetrates through the connection shell 302 and is communicated with the storage shell 301, and one side, close to an oxygen supply groove 2013, of the oxygen pipe 303 is communicated with the oxygen supply groove 2013.
In this embodiment: by arranging the oxygen supply mechanism 3, the oxygen therapy tube 303 can be externally connected with a low oxygen supply device, low oxygen is transmitted to the oxygen supply groove 2013 in the storage shell 301 through the oxygen therapy tube 303, the oxygen therapy tube 303 can be supported and protected by the connection shell 302, and low oxygen is conveniently supplied to each oxygen supply groove 2013.
As a technical optimization scheme of the utility model, positioning grooves 4 are formed in both sides and the front side of the inner wall of the placement groove 2012, positioning blocks 5 are bolted to both sides and the front side of the culture dish body 2021, the positioning blocks 5 are clamped with the positioning grooves 4, the positioning blocks 5 are matched with the culture dish body 2021, and the positioning grooves 4 are matched with the placement groove 2012.
In this embodiment: through setting up constant head tank 4 and locating piece 5, can be when culture dish body 2021 puts into standing groove 2012, because the position of locating piece 5 and constant head tank 4 is fixed, let locating piece 5 and constant head tank 4 contact and joint, can effectually avoid culture dish body 2021 to put into standing groove 2012 with wrong direction.
As a technical optimization scheme of the utility model, stabilizing grooves 6 are formed in two sides of a bearing plate 2011, stabilizing blocks 7 are bolted to two sides of the inner wall of a storage housing 301, the stabilizing grooves 6 are in sliding connection with the stabilizing blocks 7, the stabilizing blocks 7 are matched with the storage housing 301, and the stabilizing grooves 6 are matched with the bearing plate 2011.
In this embodiment: through setting up stable groove 6 and stable piece 7, let loading board 2011 when accomodating shell 301 and slide, stable groove 6 can slide on stable piece 7 to carry out spacingly to the slip of loading board 2011 at accomodating shell 301, increase the stability of loading board 2011 when accomodating shell 301 and sliding.
As a technical optimization scheme of the utility model, a sealing ring 8 is clamped at one side of the oxygen therapy tube 303 close to the oxygen supply groove 2013, anti-skid lines are carved on the surface of the sealing ring 8, and the sealing ring 8 is matched with the oxygen therapy tube 303.
In this embodiment: by providing the seal ring 8, when the oxygen therapy tube 303 is connected to the oxygen supply tank 2013, the seal ring 8 can seal the connection gap between the oxygen therapy tube 303 and the oxygen supply tank 2013, and the tightness of the connection between the oxygen therapy tube 303 and the oxygen supply tank 2013 can be increased.
As a technical optimization scheme of the utility model, a storage groove 9 is formed in the top of the slide plate cover 2022, and a poking plate 10 is rotatably connected to two sides of the inner wall of the storage groove 9, and the poking plate 10 is matched with the slide plate cover 2022 for use.
In this embodiment: through setting up storage tank 9 and plectrum 10, can be when needs slide cover 2022 from culture dish body 2021 and take off, the user can pinch plectrum 10 and slide cover 2022, and plectrum 10 can be convenient for the user slide cover 2022 to when needs put into storage shell 301 with culture dish body 2021, put into storage tank 9 with plectrum 10, can avoid plectrum 10 and take place to contact with storage shell 301.
As a technical optimization scheme of the utility model, the front side of the slide plate cover 2022 is clamped with the sealing gasket 11, the sealing gasket 11 is made of rubber material, anti-skid lines are carved on the surface of the sealing gasket 11, and the sealing gasket 11 is matched with the slide plate cover 2022 for use.
In this embodiment: by providing the gasket 11, it is possible to seal the vessel body 2021 with the muscle increasing slide cover 2022 when the slide cover 2022 is closed with the vessel body 2021.
As a technical optimization scheme of the utility model, sliding grooves 12 are formed in two sides of the inner wall of the culture dish body 2021, sliding plates 13 are bolted to two sides of the sliding plate cover 2022, the sliding grooves 12 are connected with the sliding plates 13 in a sliding manner, the sliding grooves 12 are matched with the culture dish body 2021, and the sliding plates 13 are matched with the sliding plate cover 2022.
In this embodiment: by arranging the sliding groove 12 and the sliding plate 13, the sliding plate 13 can slide in the sliding groove 12 when the sliding plate cover 2022 slides in the culture dish body 2021, so that the sliding groove 12 limits the sliding plate 13, and the sliding plate cover 2022 slides along with the sliding plate 13, thereby increasing the stability of the sliding plate cover 2022 when sliding on the culture dish body 2021.
Working principle: firstly, stem cells to be cultured are placed into a culture dish body 2021, at most, four different stem cells can be placed into four culture dish bodies 2021 respectively, a slide plate cover 2022 is closed on the culture dish bodies 2021, the culture dish bodies 2021 are placed into a placing groove 2012 on a bearing plate 2011, the bearing plate 2011 is placed into a containing shell 301 in the stem cell hypoxia incubator body 1, an hypoxia supply device is externally connected with an hypoxia supply pipe 303, the hypoxia supply device conveys hypoxia into the hypoxia supply pipe 303, the hypoxia supply pipe 303 conveys hypoxia into an oxygen supply groove 2013 along a connecting shell 302, and the oxygen supply groove 2013 conveys hypoxia into each culture dish body 2021 along an air inlet hole 2023, so that the culture dish bodies 2021 are always kept in a hypoxia state, when the culture dish bodies 2021 need to be replaced, the culture dish bodies 2021 need to be replaced are taken out from the placing groove 2012, and the replaced culture dish bodies 2021 can be replaced in the placing groove 2012.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.
Claims (8)
1. The utility model provides a stem cell hypoxia culture device, includes stem cell hypoxia incubator body (1), cultivates mechanism (2) and oxygen suppliment mechanism (3), its characterized in that: the culture mechanism (2) is connected to the inner wall of the stem cell hypoxia incubator body (1) in a sliding way, the oxygen supply mechanism (3) is bolted to the rear side of the stem cell hypoxia incubator body (1), and the rear side of the culture mechanism (2) is communicated with the oxygen supply mechanism (3);
culture mechanism (2) include base subassembly (201) and hold subassembly (202), base subassembly (201) include loading board (2011), standing groove (2012) and oxygen supply groove (2013), loading board (2011) sliding connection is at the inner wall of stem cell hypoxia incubator body (1), the top at loading board (2011) is seted up in standing groove (2012), the rear side at standing groove (2012) inner wall is seted up in oxygen supply groove (2013), hold subassembly (202) including culture dish body (2021), slide lid (2022) and income gas pocket (2023), the inner wall of culture dish body (2021) joint at standing groove (2012), slide lid (2022) sliding connection is at the top of culture dish body (2021), the rear side at culture dish body (2021) is seted up to income gas pocket (2023).
2. The stem cell hypoxia culture device according to claim 1, wherein: the oxygen supply mechanism (3) comprises a storage shell (301), a connecting shell (302) and an oxygen therapy tube (303), wherein the storage shell (301) is bolted to the inner wall of the stem cell hypoxia incubator body (1), the inner wall of the storage shell (301) is in sliding connection with a bearing plate (2011), the connecting shell (302) is bolted to the rear side of the stem cell hypoxia incubator body (1), the oxygen therapy tube (303) is communicated with the connecting shell (302), the oxygen therapy tube (303) penetrates through the connecting shell (302) to be communicated with the storage shell (301), and one side, close to an oxygen supply groove (2013), of the oxygen therapy tube (303) is communicated with the oxygen supply groove (2013).
3. The stem cell hypoxia culture device according to claim 1, wherein: positioning grooves (4) are formed in the two sides and the front side of the inner wall of the placing groove (2012), positioning blocks (5) are bolted to the two sides and the front side of the culture dish body (2021), the positioning blocks (5) are clamped with the positioning grooves (4), the positioning blocks (5) are matched with the culture dish body (2021), and the positioning grooves (4) are matched with the placing groove (2012).
4. The stem cell hypoxia culture device according to claim 2, wherein: the stabilizing device is characterized in that stabilizing grooves (6) are formed in two sides of the bearing plate (2011), stabilizing blocks (7) are bolted to two sides of the inner wall of the containing shell (301), the stabilizing grooves (6) are connected with the stabilizing blocks (7) in a sliding mode, the stabilizing blocks (7) are matched with the containing shell (301), and the stabilizing grooves (6) are matched with the bearing plate (2011).
5. The stem cell hypoxia culture device according to claim 2, wherein: one side of the oxygen therapy pipe (303) close to the oxygen supply groove (2013) is clamped with a sealing ring (8), anti-slip lines are carved on the surface of the sealing ring (8), and the sealing ring (8) is matched with the oxygen therapy pipe (303).
6. The stem cell hypoxia culture device according to claim 1, wherein: the top of slide lid (2022) has seted up and has accomodate groove (9), the both sides of accomodating groove (9) inner wall rotate and are connected with and dial board (10), dial board (10) and slide lid (2022) cooperation use.
7. The stem cell hypoxia culture device according to claim 1, wherein: the front side joint of slide lid (2022) has sealed pad (11), sealed pad (11) are made by rubber material, the surface of sealed pad (11) is carved with anti-skidding line, sealed pad (11) and slide lid (2022) cooperation use.
8. The stem cell hypoxia culture device according to claim 1, wherein: sliding tray (12) have all been seted up to the both sides of culture dish body (2021) inner wall, slide board (13) have all been bolted to the both sides of slide lid (2022), sliding tray (12) and slide board (13) sliding connection, sliding tray (12) and culture dish body (2021) cooperation use, slide board (13) and slide lid (2022) cooperation use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223049074.3U CN219117462U (en) | 2022-11-16 | 2022-11-16 | Stem cell hypoxia culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223049074.3U CN219117462U (en) | 2022-11-16 | 2022-11-16 | Stem cell hypoxia culture device |
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| Publication Number | Publication Date |
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| CN219117462U true CN219117462U (en) | 2023-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202223049074.3U Active CN219117462U (en) | 2022-11-16 | 2022-11-16 | Stem cell hypoxia culture device |
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| CN (1) | CN219117462U (en) |
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- 2022-11-16 CN CN202223049074.3U patent/CN219117462U/en active Active
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