CN219117461U

CN219117461U - Culture device for inducing mature adipocyte dedifferentiation

Info

Publication number: CN219117461U
Application number: CN202220748024.5U
Authority: CN
Inventors: 杨晓宇; 吴蕾; 孟平; 曾子晗; 刘家惠
Original assignee: Fuyang Normal University
Current assignee: Fuyang Normal University
Priority date: 2022-03-23
Filing date: 2022-03-23
Publication date: 2023-06-02
Anticipated expiration: 2032-03-23

Abstract

The utility model belongs to the technical field of cell culture devices, in particular to a culture device for inducing dedifferentiation of mature adipocytes, which aims at the problems that the existing culture method is inconvenient for gas exchange inside and outside a culture dish and inconvenient for adding culture solution and affecting the culture efficiency of adipocytes during operation, and the utility model provides a scheme which comprises a shell, wherein the culture dish is placed on the inner wall of the bottom of the shell, a ceiling is slidably connected in the culture dish, exhaust holes are formed in the ceiling, disinfection boxes are fixedly arranged on two sides of the shell, an air guide box is fixedly arranged on one side, away from each other, of each of the two disinfection boxes, an air guide mechanism is arranged in the air guide box, a stirring mechanism is arranged in the disinfection box, and a motor is fixedly arranged at the top of the disinfection box. The utility model has simple operation and convenient use, can be convenient for carrying out sufficient aerobic culture on the fat cells, and can be convenient for adding culture solution and controlling the temperature.

Description

Culture device for inducing mature adipocyte dedifferentiation

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to a culture device for inducing dedifferentiation of mature adipocytes.

Background

The quantity and the volume of the fat cells not only directly influence the meat quality of livestock, but also are closely related to the health of human beings, and diseases such as diabetes, hypertension, atherosclerosis and the like caused by obesity become main killers endangering the health of human beings, and cause great threat to the survival and the development of the human beings, so that the research of the molecular mechanism of the proliferation and the differentiation of the fat cells has great significance. It is well known that differentiation of adipocytes starts from mesodermal multipotent stem cells, gradually developing into mature adipocytes through precursor adipocytes (3 stages of adipoblast, preadipocyte and immature adipocyte). Mature adipocytes have been considered to be terminally differentiated cells and have no division and proliferation capacity and utility value. However, recent studies have found that when mature adipocytes are cultured by the ceiling method, their phenotype is reversed, spontaneously dedifferentiated into DFAT cells having proliferation capacity, and that such cells can differentiate in vitro in various directions including adipogenic, chondrogenic, osteogenic, myogenic, neurogenic, angiogenic, and the like. Meanwhile, since dedifferentiated adipose (DFAT) cells are more readily available than bone marrow-derived mesenchymal stem cells, have higher purity than adipose-derived stromal cells, DFAT cells have become an ideal cell source for tissue engineering, and have achieved research results in a number of animal models. Therefore, DFAT cells that better culture mature adipocytes in vitro to a dedifferentiated state are new developments being studied today. In view of this, the present utility model uses the characteristic that mature adipocytes have buoyancy, and dedifferentiated adipocytes recover their adherent capacity to dedifferentiate them in culture flasks, called "ceiling" method.

When the existing culture method is operated, gas exchange inside and outside the culture dish is inconvenient, and the addition of culture solution is inconvenient, so that the culture efficiency of the fat cells is affected.

Disclosure of Invention

The utility model aims to solve the defects that the prior culture method is inconvenient for gas exchange inside and outside a culture dish and inconvenient for adding culture solution and influences the culture efficiency of adipocytes during operation, and provides a culture device for inducing dedifferentiation of mature adipocytes.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a culture apparatus for inducing mature adipocyte dedifferentiation, includes the casing, the culture dish has been placed on the bottom inner wall of casing, sliding connection has the ceiling on the culture dish, has seted up the exhaust hole on the ceiling, the equal fixed mounting in both sides of casing has the disinfection case, and the equal fixed mounting in one side that two disinfection cases kept away from each other has the wind-guiding box, is equipped with wind-guiding mechanism in the wind-guiding box, be equipped with rabbling mechanism in the disinfection case, the top fixed mounting of disinfection case has the motor, and the output shaft of motor is connected with rabbling mechanism, the apron is installed at the top of casing, sliding connection has the passage on the apron, the top threaded connection of passage has the cage, the outlet duct is installed at the top of apron, is equipped with the check valve on the outlet duct.

Preferably, the air guide mechanism comprises a fixed rod, fixed rod fixed mounting is on the inner wall of air guide box, and rotate on the fixed rod and be connected with the pivot, the one end fixed mounting of pivot has the flabellum, pivot and the inner wall rotation of air guide box are connected, fixed mounting has the filter on the inner wall of air guide box, the filter mutually supports with the flabellum, the intake pipe is installed to one side of air guide box, the bottom of intake pipe extends to the liquid level of disinfect box under, one side rotation of disinfect box is connected with the transfer line, the transfer line is connected with the bull stick transmission, and fixed cover is equipped with first belt pulley on the transfer line, fixed cover is equipped with the second belt pulley in the pivot, the transmission is connected with same belt on first belt pulley and the second belt pulley, fixed mounting has the worm on the bull stick, fixed mounting has the worm wheel on the transfer line, worm and the intermeshing of worm wheel and worm wheel drive the transfer line rotates through the intermeshing of worm, the transfer line of rotation drives the flabellum through first belt pulley, the transmission connection of second belt pulley and belt rotates.

Preferably, the stirring mechanism comprises a rotating rod, the rotating rod is rotatably arranged on the inner wall of the top of the sterilizing box, a plurality of stirring rods are fixedly arranged on the rotating rod, the rotating rod is fixedly connected with a corresponding motor output shaft, an electric heating block is arranged on the inner wall of one side of the sterilizing box, an ultraviolet irradiation lamp is arranged on the inner wall of one side of the sterilizing box, the ultraviolet irradiation lamp is matched with sterilizing liquid in the sterilizing box to sterilize the entering gas, and the effect is better under the action of the stirring rods.

Preferably, the guide hole has been seted up to one side of disinfect box, guide hole and casing intercommunication each other, fixed mounting has fixed pipe on the bottom inner wall of culture dish, a plurality of ventholes have been seted up at the top of fixed pipe, the connecting pipe is installed at the top of fixed pipe, same air duct is installed on the top of two connecting pipes, the air duct is mutually supported with two guide holes, connecting pipe and ceiling sliding connection, the gas in the disinfect box passes through the mutually supported of guide hole and air duct to can enter into in the culture dish, thereby cultivate inside cell.

Compared with the prior art, the utility model has the advantages that:

(1) In the scheme, the worm is meshed with the worm wheel, and the first belt pulley, the second belt pulley and the belt are in transmission connection to drive the rotating shaft to rotate, so that air can be introduced into the sterilizing box through the fan blades;

(2) Due to the effect of the ultraviolet irradiation lamp and the disinfectant in the disinfection box, the gas in the disinfection box is sufficiently disinfected through the effect of the stirring rod.

The utility model has simple operation and convenient use, can be convenient for carrying out sufficient aerobic culture on the fat cells, and can be convenient for adding culture solution and controlling the temperature.

Drawings

FIG. 1 is a schematic three-dimensional structure of a culture apparatus for inducing dedifferentiation of mature adipocytes according to the present utility model;

FIG. 2 is a schematic diagram showing a cross-sectional front view of a culture apparatus for inducing dedifferentiation of mature adipocytes according to the present utility model;

FIG. 3 is a schematic three-dimensional structure of a fixing tube and a connecting tube of a culture device for inducing dedifferentiation of mature adipocytes according to the present utility model;

FIG. 4 is a schematic diagram of the structure of part A of a culture apparatus for inducing dedifferentiation of mature adipocytes according to the present utility model;

FIG. 5 is a schematic diagram showing the structure of part B of a culture apparatus for inducing dedifferentiation of mature adipocytes according to the present utility model.

In the figure: 1. a housing; 2. a culture dish; 3. a ceiling; 4. an exhaust hole; 5. a sterilization box; 6. an air guide box; 7. a filter plate; 8. a rotating rod; 9. a stirring rod; 10. a motor; 11. an air inlet pipe; 12. a transmission rod; 13. a worm; 14. a worm wheel; 15. a fixed rod; 16. a rotating shaft; 17. a fan blade; 18. a guide hole; 19. an air duct; 20. a connecting pipe; 21. a fixed tube; 22. an air outlet hole; 23. a material guiding pipe; 24. a cover plate; 25. an air outlet pipe; 26. an electric heating block; 27. ultraviolet irradiation lamp.

Detailed Description

The technical solutions of the present embodiment will be clearly and completely described below with reference to the drawings in the present embodiment, and it is apparent that the described embodiments are only some embodiments of the present embodiment, not all embodiments.

Referring to fig. 1-5, a culture device for inducing mature adipocyte dedifferentiation comprises a housing 1, wherein a culture dish 2 is placed on the inner wall of the bottom of the housing 1, a ceiling 3 is slidably connected in the culture dish 2, an exhaust hole 4 is formed in the ceiling 3, disinfection boxes 5 are fixedly installed on two sides of the housing 1, an air guide box 6 is fixedly installed on one side, away from each other, of each disinfection box 5, an air guide mechanism is arranged in the air guide box 6, a stirring mechanism is arranged in the disinfection boxes 5, a motor 10 is fixedly installed at the top of each disinfection box 5, an output shaft of the motor 10 is connected with the stirring mechanism, a cover plate 24 is installed at the top of the housing 1, a guide pipe 23 is slidably connected on the cover plate 24, an isolation cover is connected to the top of the guide pipe 23 in a threaded manner, an air outlet pipe 25 is installed at the top of the cover plate 24, and a one-way valve is arranged on the air outlet pipe 25.

In this embodiment, air guide mechanism includes dead lever 15, dead lever 15 fixed mounting is on the inner wall of air guide box 6, and rotationally be connected with pivot 16 on the dead lever 15, the one end fixed mounting of pivot 16 has flabellum 17, pivot 16 and the inner wall rotation of air guide box 6 are connected, fixedly mounted has filter 7 on the inner wall of air guide box 6, filter 7 and flabellum 17 mutually support, intake pipe 11 is installed to one side of air guide box 6, the bottom of intake pipe 11 extends to under the liquid level of disinfect box 5, one side rotation of disinfect box 5 is connected with transfer line 12, transfer line 12 and bull stick 8 transmission are connected, and the fixed cover is equipped with first belt pulley on transfer line 12, fixed cover is equipped with the second belt pulley on the pivot 16, the transmission is connected with same belt on first belt pulley and the second belt pulley, fixedly mounted worm 13 on the bull stick 8, fixedly mounted has worm wheel 14 on transfer line 12, worm 13 and corresponding worm 13 intermesh, the intermeshing of

worm

13 and 14 drive transfer line 12, the transmission line 12 is rotated through the intermeshing of worm 13, transfer line 12 is driven through first belt pulley, second belt pulley is connected with the transmission 17.

In this embodiment, rabbling mechanism includes bull stick 8, and bull stick 8 rotates to be installed on the top inner wall of disinfect box 5, and fixed mounting has a plurality of stirring rod 9 on the bull stick 8, and bull stick 8 and the motor 10 output shaft fixed connection who corresponds installs electric heat piece 26 on the inner wall of one side of disinfect box 5, and installs ultraviolet irradiation lamp 27 on the inner wall of one side of disinfect box 5, and the gaseous disinfection of antiseptic solution in ultraviolet irradiation lamp 27 cooperation disinfect box 5 to effect is better under the effect of stirring rod 9.

In this embodiment, guide hole 18 has been seted up to one side of disinfect box 5, guide hole 18 and casing 1 intercommunication each other, fixed mounting has fixed pipe 21 on the bottom inner wall of culture dish 2, a plurality of ventholes 22 have been seted up at the top of fixed pipe 21, connecting pipe 20 is installed at the top of fixed pipe 21, same air duct 19 is installed on the top of two connecting pipes 20, air duct 19 and two guide holes 18 mutually support, connecting pipe 20 and ceiling 3 sliding connection, the gas in the disinfect box 5 passes through the mutually support of guide hole 18 and air duct 19 to can enter into in the culture dish 2, thereby cultivate inside cell.

The theory of operation, during operation, the ceiling 3 floats on the culture liquid level under the effect of culture liquid in culture dish 2, and the adipocyte in the culture liquid adsorbs and cultivates in the ceiling 3 bottom, the setting of guide pipe 23 simultaneously can carry out the leading-in to the culture liquid, the start motor 10 switch, the output shaft of motor 10 drives the bull stick 8 and rotates, the bull stick 8 passes through worm 13 and worm wheel 14's intermeshing drive transfer line 12 rotates, pivoted transfer line 12 passes through first belt pulley, the transmission connection of second belt pulley and belt drives pivot 16 and rotates, pivoted pivot 16 drives flabellum 17 and carries out the leading-in gas in the disinfection case 5, and carry out disinfection under the effect of disinfection case 5 disinfection and ultraviolet irradiation lamp 27 through disinfection case 5, simultaneously pivoted bull stick 8 stirs the mixture to the gas in the disinfection case 5 through stirring stick 9, thereby make disinfection more abundant, the gas after the disinfection enters into fixed pipe 21 through guide hole 18 and air duct 19 simultaneously, and discharge hole 22 into culture dish 2 in, thereby can increase the aerobic culture of culture 2 cells, the gas after the use carries out the exhaust hole 4 and exhaust hole 25 simultaneously and carries out the setting up and can carry out the temperature to heat up to the culture liquid to the disinfection case 5.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art will be able to apply equally to the technical solution of the present utility model and the inventive concept thereof, within the scope of the present utility model.

Claims

1. The utility model provides a culture apparatus for inducing mature adipocyte dedifferentiation, includes casing (1), its characterized in that, culture dish (2) have been placed on the bottom inner wall of casing (1), sliding connection has ceiling (3) in culture dish (2), has seted up exhaust hole (4) on ceiling (3), the equal fixed mounting in both sides of casing (1) has disinfect box (5), and one side that two disinfect boxes (5) kept away from each other is fixed mounting has wind-guiding box (6), is equipped with wind-guiding mechanism in wind-guiding box (6), be equipped with rabbling mechanism in disinfect box (5), the top fixed mounting of disinfect box (5) has motor (10), and the output shaft of motor (10) is connected with rabbling mechanism, apron (24) are installed at the top of casing (1), sliding connection has drain pipe (23) on apron (24), the top threaded connection of drain pipe (23) has the cage, outlet duct (25) are installed at the top of apron (24), are equipped with the check valve on outlet duct (25).

2. The culture device for inducing the dedifferentiation of mature adipocytes according to claim 1, characterized in that the air guide mechanism comprises a fixing rod (15), the fixing rod (15) is fixedly installed on the inner wall of the air guide box (6), a rotating shaft (16) is rotatably connected to the fixing rod (15), one end of the rotating shaft (16) is fixedly provided with a fan blade (17), the rotating shaft (16) is rotatably connected with the inner wall of the air guide box (6), a filter plate (7) is fixedly installed on the inner wall of the air guide box (6), the filter plate (7) is matched with the fan blade (17), an air inlet pipe (11) is installed on one side of the air guide box (6), and the bottom end of the air inlet pipe (11) extends to the position below the liquid surface of the sterilizing box (5).

3. The culture device for inducing dedifferentiation of mature adipocytes according to claim 1, characterized in that the stirring mechanism comprises a rotating rod (8), the rotating rod (8) is rotatably mounted on the top inner wall of the sterilizing box (5), a plurality of stirring rods (9) are fixedly mounted on the rotating rod (8), and the rotating rod (8) is fixedly connected with the output shaft of a corresponding motor (10).

4. A culture device for inducing dedifferentiation of mature adipocytes according to claim 3, characterized in that one side of the sterilization box (5) is rotatably connected with a transmission rod (12), the transmission rod (12) is in transmission connection with the rotating rod (8), a first belt pulley is fixedly sleeved on the transmission rod (12), a second belt pulley is fixedly sleeved on the rotating shaft (16), and the first belt pulley and the second belt pulley are in transmission connection with the same belt.

5. The culture device for inducing dedifferentiation of mature adipocytes according to claim 4, characterized in that a worm (13) is fixedly installed on the rotating rod (8), a worm wheel (14) is fixedly installed on the driving rod (12), and the worm (13) is engaged with the corresponding worm wheel (14).

6. A culture apparatus for inducing dedifferentiation of mature adipocytes according to claim 1, characterized in that an electric heating block (26) is mounted on one side inner wall of the sterilization case (5), and an ultraviolet irradiation lamp (27) is mounted on one side inner wall of the sterilization case (5).

7. The culture device for inducing the dedifferentiation of mature adipocytes according to claim 1, characterized in that a guide hole (18) is provided at one side of the sterilization box (5), the guide hole (18) is communicated with the housing (1), a fixed tube (21) is fixedly installed on the inner wall of the bottom of the culture dish (2), a plurality of air outlet holes (22) are provided at the top of the fixed tube (21), a connecting tube (20) is installed at the top of the fixed tube (21), the same air guide tube (19) is installed at the top of the two connecting tubes (20), the air guide tube (19) is matched with the two guide holes (18), and the connecting tube (20) is in sliding connection with the ceiling (3).