CN219050410U

CN219050410U - Amino acid chromatographic separation column

Info

Publication number: CN219050410U
Application number: CN202223377657.9U
Authority: CN
Inventors: 顾培培
Original assignee: Jiangsu Huameichen Instrument Technology Co ltd
Current assignee: Jiangsu Huameichen Instrument Technology Co ltd
Priority date: 2022-12-14
Filing date: 2022-12-14
Publication date: 2023-05-23
Anticipated expiration: 2032-12-14

Abstract

The utility model discloses an amino acid chromatographic separation column which comprises a column body, wherein a thread strip is fixedly arranged at the outer side of the bottom end of the column body, a lower sealing cover is connected with the outer side of the thread strip in a threaded manner, a lower supporting block is fixedly arranged at the inner side of the lower sealing cover, a lower sealing ring is fixedly arranged at the inner side of the lower sealing cover, a lower inner annular wall is arranged in the lower supporting block, a branch pipe is fixedly arranged at the bottom of an inner cavity of the lower inner annular wall, a plurality of notches are formed in the branch pipe, and a liquid outlet is formed in the lower supporting block. Through the interior post and the urceolus that set up, when last closing cap is dismantled, the interior post produces certain atmospheric pressure under the effect of spacing piston and air chamber, forms certain pulling force, stabilizes interior post and urceolus in a position to handing-over rotation between interior post and the last closing cap makes the last closing cap can be spacing in certain position, has avoided losing, makes whole use stable, the moment of being convenient for is stable.

Description

Amino acid chromatographic separation column

Technical Field

The utility model belongs to the technical field of chromatographic separation columns, and particularly relates to an amino acid chromatographic separation column.

Background

Chromatography columns can be divided into packed columns and open-tube columns. Most of them are made of metal or glass. There are straight pipe, coil pipe, U-shaped pipe, etc. Liquid chromatography generally employs packed columns, the separation effect of which depends on the stationary phase chosen, as well as the preparation and operating conditions of the column. Chromatography is a separation analysis means, separation is the core, and thus the chromatographic column responsible for the separation is the heart of the chromatographic system. The requirements for chromatographic columns are high column efficiency, good selectivity, high analysis speed and the like. Various particulate fillers commercially available for HPLC such as porous silica gel and silica gel-based bonding phases, alumina, organic polymer microspheres (including ion exchange resins), porous carbon, etc., generally have particle sizes of 3,5,7,10 μm, etc., and column efficiency theoretical values of 5 to 16 ten thousand/meter can be achieved.

In the use process of the chromatographic separation column, as the adsorption force of the stationary phase adsorbent to different components is different, the mixed components flow through the stationary phase adsorbent at different rates, so that the purpose of separation is achieved, when the separation column with the stationary phase adsorbent needs to be replaced is used, the operation trouble and the low efficiency are easy to generate when the new stationary phase adsorbent is replaced, and meanwhile, the separation state of a plugging piece of a plurality of chromatographic columns is easy to be lost.

Disclosure of Invention

Aiming at the defects existing in the prior art, the utility model aims to provide an amino acid chromatographic separation column, which solves the problems that the operation is troublesome and the efficiency is low easily caused by replacing a new stationary phase adsorbent, and the separation state of plugging pieces of most chromatographic columns is easy to lose.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides an amino acid chromatographic separation post, includes the cylinder, the outside fixed mounting of cylinder bottom has the screw thread strip, the outside threaded connection of screw thread strip has down the closing cap, the inboard fixed mounting of closing cap has down the supporting shoe down, the inboard fixed mounting of closing cap has down the sealing ring, the inside of supporting shoe is provided with down interior rampart down, the bottom fixed mounting of interior rampart inner chamber has the branch pipe down, a plurality of notches have been seted up to the inside of branch pipe, the liquid outlet has been seted up to the inside of supporting shoe down, the top of supporting shoe is provided with the lower filter disc, the outside fixed mounting on cylinder top has the supporting ring, the top fixed mounting of supporting ring has the urceolus, the inside movable sleeve of urceolus has the inner column, the bottom fixed mounting of inner column has spacing piston, the inside of urceolus is provided with the air chamber, the top movable mounting of cylinder has the upper closing cap, the outside fixed mounting of upper closing cap has the outer loop, the inside movable grafting of outer loop has three fastening bolt, three the outside threaded connection of fastening bolt has the inside, the inside screw thread connection has the nut on the inside of supporting shoe, the inside of supporting shoe has the sealing ring, the inside is provided with the sealing ring on the inside of supporting shoe, the inside has the sealing ring.

Preferably, the specification and the size of the lower support block are matched with those of the column body, and the specification and the size of the lower sealing ring are matched with those of the column body.

By adopting the technical scheme, the sealing device has the advantages that the soft deformation of the lower sealing ring can seal the space between the lower sealing cover and the cylinder, so that the sealing device is convenient to use stably as a whole.

Preferably, the positions of the notches correspond to the positions of the lower inner annular wall, and the notches are uniformly distributed in the branch pipe in a circumferential manner.

By adopting the technical scheme, the guide device has the advantages that substances on the inner side of the lower inner annular wall are conveniently guided out, limit is reduced, and flow guide is convenient.

Preferably, the branch pipe is communicated with a liquid outlet, and the liquid outlet penetrates through the lower branch block and extends to the bottom of the lower sealing cover.

Through adopting above-mentioned technical scheme, advantage lies in being convenient for increase holistic stable in structure, is convenient for bear bigger pressure with lower filter disc, facilitate the use.

Preferably, the inner wall of the cylinder is provided with an anti-corrosion coating, and the inner wall of the cylinder is smooth.

By adopting the technical scheme, the anti-corrosion coating has the advantages of being convenient for reducing the pipe wall effect, good in using effect and capable of increasing the corrosion resistance of materials.

Preferably, the limiting piston is movably sleeved in the outer cylinder, the air chamber is positioned at the bottom of the limiting piston, and the air chamber is provided with inert gas.

By adopting the technical scheme, the inner column has the advantages that certain air pressure is generated under the action of the limiting piston and the air chamber, and certain tensile force is formed.

Preferably, the top end of the inner column is hinged to the bottom of the outer ring, and the three fastening bolts are in threaded connection with the bottom of the support ring and extend to the bottom of the support ring.

Through adopting above-mentioned technical scheme, advantage lies in the cooperation three fastening bolt that are the triangle and distribute is convenient for moment stability, is convenient for firm spacing and use whole result of use.

Compared with the prior art, the utility model has the beneficial effects that:

1. the material that will handle through the inlet is placed and is entered into the cylinder inside, enter into stationary phase adsorbent inboard through last filter disc, after adsorbing, the material is guided through lower filter disc and lower inner ring wall, derive through the liquid outlet, when needs change inside adsorbent, rotate down the closing cap and make down the closing cap gradually lift down with threaded strip's threaded connection, then can pass through rotation fastening bolt, make the spacing of closing cap remove, and lift up the closing cap, rotate after breaking away from the contact with the cylinder, both ends all can be dismantled the stationary phase adsorbent of the replacement inboard of being convenient for this moment, and is whole excellent in use effect, convenient to use.

2. Through the interior post and the urceolus that set up, when last closing cap is dismantled, the interior post produces certain atmospheric pressure under the effect of spacing piston and air chamber, forms certain pulling force, stabilizes interior post and urceolus in a position to handing-over rotation between interior post and the last closing cap makes the last closing cap can be spacing in certain position, has avoided losing, and both ends all can use this structure under the condition permission, and the cooperation is three fastening bolt's is fixed for whole use is stable, the moment of being convenient for is stable.

Drawings

FIG. 1 is a schematic view of a front perspective view of the present utility model;

FIG. 2 is a schematic diagram of a front cross-sectional structure of the present utility model;

FIG. 3 is an enlarged schematic view of the structure of FIG. 2A according to the present utility model;

fig. 4 is an enlarged schematic view of the structure of fig. 2B according to the present utility model.

In the figure: 1. a column; 2. a lower cover; 3. a support ring; 4. a liquid inlet; 5. an upper cover; 6. an outer ring; 7. a fastening bolt; 8. an upper seal ring; 9. an upper support block; 10. an upper inner annular wall; 11. an upper filter sheet; 12. a nut; 13. a branch pipe; 14. a lower filter sheet; 15. a notch; 16. a lower support block; 17. a lower inner annular wall; 18. a liquid outlet; 19. a lower seal ring; 20. a thread strip; 21. a stationary phase adsorbent; 22. an inner column; 23. an outer cylinder; 24. a limit piston; 25. an air chamber.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by one of ordinary skill in the art without undue burden on the person of ordinary skill in the art based on the embodiments of the present utility model, are within the scope of the present utility model.

As shown in fig. 1-4, an amino acid chromatographic separation column comprises a column body 1, a threaded strip 20 is fixedly arranged at the outer side of the bottom end of the column body 1, a lower sealing cover 2 is fixedly connected to the outer side of the threaded strip 20, a lower supporting block 16 is fixedly arranged at the inner side of the lower sealing cover 2, a lower sealing ring 19 is fixedly arranged at the inner side of the lower sealing cover 2, a lower inner annular wall 17 is arranged in the lower supporting block 16, a branch pipe 13 is fixedly arranged at the bottom of an inner cavity of the lower inner annular wall 17, a plurality of notches 15 are formed in the branch pipe 13, a liquid outlet 18 is formed in the lower supporting block 16, a lower filter disc 14 is arranged at the top of the lower supporting block 16, a supporting ring 3 is fixedly arranged at the outer side of the top end of the column body 1, an outer cylinder 23 is fixedly provided with an outer cylinder 23, an inner column 22 is movably sleeved at the inner side of the outer cylinder 23, a limiting piston 24 is fixedly arranged at the bottom of the inner column 22, three fastening bolts 7 are movably inserted into the inner side of the outer ring 6, three fastening bolts 12 are fixedly connected with the upper sealing ring 9, a filter disc 9 is fixedly arranged at the inner side of the fixing block 9, a sealing ring 9 is fixedly arranged at the inner side of the inner sealing ring 9, and an upper sealing ring 9 is fixedly arranged at the inner side of the inner sealing ring 9.

The working principle of the technical scheme is as follows:

when the device is used, substances to be treated are placed into the column 1 through the liquid inlet 4, enter the inner side of the stationary phase adsorbent 21 through the upper filter disc 11, flow is guided through the lower filter disc 14 and the lower inner annular wall 17 after adsorption, the substances are guided out through the liquid outlet 18, when the internal adsorbent needs to be replaced, the lower cover 2 is rotated to enable the lower cover 2 to be gradually detached under the threaded connection with the threaded strip 20, then the limit of the upper cover 5 can be released through rotating the fastening bolt 7, the upper cover 5 is lifted upwards, the stationary phase adsorbent 21 which is convenient to replace the inner side can be detached at two ends after the upper cover is separated from the contact with the column 1, and the device is good in integral use effect and convenient to use.

In another embodiment, as shown in fig. 2, the lower support block 16 has a size that matches the size of the column 1, and the lower seal ring 19 has a size that matches the size of the column 1.

The soft deformation of the lower sealing ring 19 can seal the lower sealing cover 2 and the column body 1, so that the lower sealing ring is convenient to use and stable in whole.

In another embodiment, shown in fig. 4, the notches 15 are positioned corresponding to the position of the lower inner annular wall 17, the notches 15 being circumferentially distributed uniformly inside the branch pipe 13.

The notch 15 is convenient for guiding the substances after the flow guiding treatment, is convenient to use, is convenient for guiding the substances on the inner side of the lower inner annular wall 17, reduces limit and is convenient for flow guiding.

In another embodiment, as shown in fig. 4, the manifold 13 communicates with a fluid outlet 18, the fluid outlet 18 extending through the lower manifold block 16 and to the bottom of the lower closure 2.

The branch pipes 13 provide a certain support for the lower filter disc 14 at the top, so that the support force is convenient to increase, the overall structural stability is convenient to increase, and the lower filter disc 14 is convenient to bear larger pressure and is convenient to use.

In another embodiment, as shown in FIG. 2, the inner wall of the cylinder 1 is provided with an anti-corrosion coating, and the inner wall of the cylinder 1 is smooth.

The inside of the cylinder 1 is polished, so that the cylinder is convenient to clean, the anti-corrosion coating is uniformly distributed, the pipe wall effect is convenient to reduce, the use effect is good, and the anti-corrosion coating can increase the corrosion resistance of materials.

In another embodiment, as shown in fig. 3, the limiting piston 24 is movably sleeved inside the outer cylinder 23, the air chamber 25 is positioned at the bottom of the limiting piston 24, and the air chamber 25 is provided with inert gas.

Through the interior post 22 and the urceolus 23 that set up, when last closing cap 5 is dismantled, interior post 22 produces certain atmospheric pressure under the effect of spacing piston 24 and air chamber 25, forms certain pulling force, stabilizes interior post 22 and urceolus 23 in a position to handing-over rotation between interior post 22 and the upper closing cap 5 makes upper closing cap 5 can spacing in certain position, has avoided losing, and both ends all can use this structure under the condition permission, and the fixed of cooperation three fastening bolt 7 makes whole use stable, the moment of being convenient for is stable.

In another embodiment, shown in figures 1 and 2, the top end of the inner post 22 is hinged to the bottom of the outer ring 6, and three fastening bolts 7 are threaded through the support ring 3 and extend to the bottom of the support ring 3.

The upper sealing cover 5 can rotate due to the hinging of the inner column 22, so that the device is convenient to use, is convenient to stabilize in moment due to the fact that the device is matched with three fastening bolts 7 distributed in a triangular mode, is convenient to firmly limit and has a good integral use effect.

The working principle and the using flow of the utility model are as follows: when the device is used, substances to be treated are placed into the column 1 through the liquid inlet 4, the substances enter the inner side of the stationary phase adsorbent 21 through the upper filter disc 11, after adsorption, the substances are guided through the lower filter disc 14 and the lower inner annular wall 17, and guided out through the liquid outlet 18, when the internal adsorbent needs to be replaced, the lower cover 2 is rotated to enable the lower cover 2 to be gradually detached under the threaded connection with the threaded strip 20, then the fastening bolt 7 can be rotated, the limit of the upper cover 5 is released, the upper cover 5 is lifted upwards, the upper cover rotates after being in contact with the column 1, at the moment, both ends can be detached to facilitate replacement of the inner stationary phase adsorbent 21, the device is good in overall use effect, and through the arranged inner column 22 and the outer cylinder 23, when the upper cover 5 is detached, certain air pressure is generated by the inner column 22 under the action of the limit piston 24 and the air chamber 25, certain tension is formed to enable the inner column 22 and the outer cylinder 23 to be stable at one position, and the rotation between the inner column 22 and the upper cover 5 can enable the upper cover 5 to be rotated, the joint position of the upper cover 5 to be stable, the device can be prevented from being used under certain position, and the condition that the fastening bolt is stable, the fastening torque is ensured, the fastening condition can be ensured, and the whole can be conveniently used, and stable, the whole conditions can be used, and the stable conditions can be used.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An amino acid chromatographic separation column comprising a column body (1), characterized in that: the utility model discloses a movable plug-in type air chamber sealing device, including cylinder (1) and outer ring (6) and is characterized in that screw thread strip (20) is fixedly arranged on the outer side of the bottom end of cylinder (1), lower sealing cover (2) is connected with outside screw thread of screw thread strip (20), lower supporting block (16) is fixedly arranged on the inner side of lower sealing cover (2), lower sealing ring (19) is fixedly arranged on the inner side of lower sealing cover (2), lower inner annular wall (17) is arranged in the inner part of lower supporting block (16), branch pipe (13) is fixedly arranged at the bottom of the inner cavity of lower inner annular wall (17), a plurality of notches (15) are formed in the inner part of branch pipe (13), liquid outlet (18) is formed in the inner part of lower supporting block (16), lower filter disc (14) is arranged at the top of lower supporting block (16), supporting ring (3) is fixedly arranged on the outer side of the top of cylinder (1), outer cylinder (23) is fixedly arranged on the top of supporting ring (3), inner column (22) is movably sleeved with inner cylinder (23), limiting piston (24) is fixedly arranged at the bottom of inner cylinder (22), inner cylinder (23), inner cylinder (25) is fixedly sleeved with inner cylinder (5), and the inner sealing cover (6) is fixedly arranged on the top of outer cylinder (6), the three outside threaded connection of fastening bolt (7) has nut (12), the inboard fixed mounting of going up closing cap (5) has last piece (9), the inside of going up piece (9) is provided with inlet (4), the inside of going up piece (9) is provided with interior rampart (10), the bottom of going up piece (9) is provided with filter disc (11), the inboard fixed mounting of going up closing cap (5) has last sealing ring (8), the inside of cylinder (1) is provided with stationary phase adsorbent (21).

2. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the specification and the size of the lower support block (16) are matched with those of the column body (1), and the specification and the size of the lower sealing ring (19) are matched with those of the column body (1).

3. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the positions of the notches (15) correspond to the positions of the lower inner annular wall (17), and the notches (15) are uniformly distributed in the branch pipes (13) in a circumferential manner.

4. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the branch pipe (13) is communicated with a liquid outlet (18), and the liquid outlet (18) penetrates through the lower branch block (16) and extends to the bottom of the lower sealing cover (2).

5. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the inner wall of the cylinder (1) is provided with an anti-corrosion coating, and the inner wall of the cylinder (1) is smooth.

6. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the limiting piston (24) is movably sleeved in the outer cylinder (23), the air chamber (25) is positioned at the bottom of the limiting piston (24), and the air chamber (25) is provided with inert gas.

7. An amino acid chromatographic separation column in accordance with claim 1, characterized in that: the top end of the inner column (22) is hinged to the bottom of the outer ring (6), and three fastening bolts (7) are in threaded connection with the support ring (3) and extend to the bottom of the support ring (3).