CN219032148U

CN219032148U - Heating mechanism of nucleic acid extraction instrument and nucleic acid extraction instrument

Info

Publication number: CN219032148U
Application number: CN202223507430.1U
Authority: CN
Inventors: 胡祥; 李晓峰
Original assignee: Guangdong Runpon Bioscience Co Ltd
Current assignee: Guangdong Runpon Bioscience Co Ltd
Priority date: 2022-12-26
Filing date: 2022-12-26
Publication date: 2023-05-16
Anticipated expiration: 2032-12-26

Abstract

The utility model provides a heating mechanism of a nucleic acid extractor and the nucleic acid extractor, which belong to the technical field of detection equipment, and the heating mechanism of the nucleic acid extractor comprises: a heating element; the heat conducting seat is connected with the heating element and is provided with a plurality of bulges; the lower surface of the deep pore plate is provided with a groove, and the groove is matched with the protrusion. The device transmits the heat of the heating element to the deep hole plate through the heat conducting seat, a plurality of bulges are arranged on the heat conducting seat, the bottom of the deep hole plate is also provided with grooves matched with the bulges, and the heat conducting seat heats the deep hole plate from the bottom and also heats the deep hole plate from the inside through the contact of the grooves and the bulges, so that the heating is more uniform, the contact area between the heat conducting seat and the deep hole plate is increased by phase change, the heating speed is high, the time is saved, and the efficiency of the analysis process is effectively improved.

Description

Heating mechanism of nucleic acid extraction instrument and nucleic acid extraction instrument

Technical Field

The utility model relates to the technical field of detection equipment, in particular to a heating mechanism of a nucleic acid extractor and the nucleic acid extractor.

Background

In the fields of biotechnology and chemical analysis, the more perfect the sample analysis process is, the most time-consuming sample processing is an extremely important link, and the precision and accuracy of subsequent analysis and detection are affected. During processing, it is often necessary to heat the sample.

At present, heating in sample pretreatment is mainly realized by heating devices such as an electric furnace, an electric heating plate, a microwave oven, a water bath, a metal bath and the like. The heating devices are used for heating the bottom of the deep hole plate for containing the sample, so that the sample is heated.

However, when the sample in the large-capacity deep hole plate is heated, the phenomena of slow heating speed and uneven heating temperature can occur due to the large sample quantity, thereby influencing the efficiency of the whole analysis process.

Disclosure of Invention

Therefore, the technical problem to be solved by the utility model is to overcome the defect of uneven heating of the deep hole plate in the prior art, thereby providing a heating mechanism of a nucleic acid extractor and the nucleic acid extractor.

A heating mechanism for a nucleic acid extractor, comprising:

a heating element;

the heat conduction seat of the main heating plate is connected with the heating element and comprises main heating plates, and the main heating plates are provided with platy protrusions distributed in rows at intervals;

the deep hole plate, be provided with the recess between every row of reaction hole of deep hole plate bottom, the recess can with the closely laminating of platy protrusion.

Preferably, the plate-shaped protrusion is provided with a fastening position, and the deep hole plate is provided with a joint position.

As a preferable scheme, the heat conduction seat further comprises a side heating plate, wherein the side heating plate is arranged on the side edge of the main heating plate and is perpendicular to the extending direction of the platy protrusions;

preferably, the main heating plate is detachably connected with the heating element.

As a preferable scheme, the main heating plate is pre-buried at the bottom of the deep hole plate, and the main heating plate and the deep hole plate are integrally formed.

Preferably, the deep hole plate is a 48-hole plate, and adjacent plate-shaped protrusions are closely attached to grooves on two sides of each row of reaction holes of the 48-hole plate.

Preferably, the deep hole plate is a 96-hole plate, and adjacent plate-shaped protrusions are tightly attached to grooves on two sides of each two rows of reaction holes of the 96-hole plate.

Preferably, the method further comprises:

a heat shield connected to the bottom of the heating element; the heat shield is provided with a heat shield slot, and the heating element is disposed in the heat shield slot.

Preferably, the method further comprises:

a base coupled to the heat shield, the base adapted to house the heat shield;

according to another aspect of the present application, there is also provided a nucleic acid extractor including the heating mechanism of the nucleic acid extractor.

The technical scheme of the utility model has the following advantages:

1. according to the utility model, the plate-shaped protrusions 3 arranged in rows/columns on the main heating plate are matched with the grooves at the bottom of the matched deep-hole plate so as to heat liquid in the deep-hole plate, and compared with the structure of fully wrapping the reaction holes at the bottom of the deep-hole plate, the protrusions arranged in rows/columns on the main heating plate can ensure that the redundant arrangement of the heating plates is reduced on the premise of realizing the heating effect, meanwhile, the device can also be compatible with heating 48-hole plates and 96-hole plates, has a simple structure, saves cost and improves practicability.

2. According to the utility model, the heat of the heating element is transferred to the deep hole plate through the heat conduction seat main heating plate, the heat conduction seat main heating plate is provided with a plurality of plate-shaped protrusions, the bottom of the deep hole plate is also provided with grooves matched with the plate-shaped protrusions, and the heat conduction seat main heating plate heats the grooves in the deep hole plate through the contact of the grooves and the plate-shaped protrusions, so that the heat conduction seat main heating plate heats the deep hole plate from the bottom and also heats the deep hole plate from the inside, the heating is more uniform, and the influence of heat transfer faults generated by adopting too many heat conductors on the heating result is avoided.

2. The heating mechanism of the nucleic acid extractor provided by the utility model has the advantages that the main heating plate is pre-buried at the bottom of the pore plate and is integrally formed with the pore plate, so that the influence of heat transfer faults generated by too many heat conductors on the heating result can be avoided.

3. The heating mechanism of the nucleic acid extractor is also provided with the heat shield, the heat shield is provided with the heat insulation groove, the heating element is placed in the heat insulation groove, the heat shield is generally made of materials with poor heat conduction performance, the heat shield can effectively isolate the heat of the heating element, the phase change is realized, the heating speed of the heating element is increased, and the working efficiency of the whole device is improved.

4. The heating mechanism of the nucleic acid extractor is also provided with the base, the heat shield is placed on the base, the heat shield can be placed on most of work tables through the base, and the base is supported on the ground through four legs, so that the whole device is more stable.

5. The heating mechanism of the nucleic acid extractor provided by the utility model is characterized in that aluminum is selected as the material of the main heating plate of the heat conduction seat, so that the main heating plate of the heat conduction seat has good heat conduction performance, the cost of the main heating plate of the heat conduction seat is lower, and the main heating plate of the heat conduction seat is convenient for large-scale production and use.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram showing the overall structure of a heating mechanism of a nucleic acid extractor according to the present utility model.

Fig. 2 is a schematic diagram of the structure of a deep well plate of a heating mechanism of the nucleic acid extractor provided by the utility model.

FIG. 3 is a cross-sectional view of a deep well plate of a heating mechanism of a nucleic acid extractor according to the present utility model.

Fig. 4 is a schematic diagram of the structure of a deep well plate of a heating mechanism of a nucleic acid extractor according to the present utility model.

Fig. 5 is a schematic structural view of a heating element of a heating mechanism of a nucleic acid extracting apparatus according to the present utility model.

Reference numerals illustrate:

1. a heating element; 2. a heat conduction seat; 21. side heating plates; 22. a main heating plate; 3. a plate-like protrusion; 31. a buckling position; 4. a deep well plate; 5. a groove; 6. a thermal protection switch; 7. a temperature control sensor; 8. a heat shield; 9. and (5) a base.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In addition, the technical features of the different embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.

Example 1

As shown in FIG. 1, the heating mechanism of the nucleic acid extractor provided by the utility model comprises: a heating element 1; the heat conduction seat 2 is connected with the heating element 1, the heat conduction seat 2 comprises a main heating plate, and plate-shaped protrusions 3 distributed in rows are arranged at intervals on the main heating plate; a deep hole plate 4, wherein a groove 5 is arranged between each row of reaction holes at the bottom of the deep hole plate, and the groove 5 can be tightly attached to the plate-shaped protrusions 33; the device transmits the heat of the heating element 1 to the deep hole plate 4 through the heat conduction seat 2, a plurality of plate-shaped protrusions 3 are arranged on the heat conduction seat 2, grooves 5 matched with the plate-shaped protrusions 3 are also arranged at the bottom of the deep hole plate 4, the heat conduction seat 2 heats the grooves 5 inside the deep hole plate 4 through the contact of the grooves 5 and the plate-shaped protrusions 3, the heat conduction seat 2 heats the deep hole plate 4 from the bottom and also heats the deep hole plate 4 from the inside, so that the heating is more uniform, and the influence of heat transmission faults generated by too many heat conductors on a heating result is avoided.

As shown in fig. 2-3, in the present device, the depth of the groove 5 on the lower surface of the deep hole plate 4 also affects the heating speed, but the opening of the groove 5 also occupies the space of the deep hole plate 4 and affects the volume in each hole of the deep hole plate 4, in this embodiment, the depth of the groove 5 is set to be about half of the height of the deep hole plate 4, and in order to increase the heating speed of the deep hole plate 4, the groove 5 of the deep hole plate 4 may be lengthened. As shown in fig. 4, in order to facilitate the production and the mass production, the grooves 5 are disposed between each row of reaction holes at the bottom of the deep-hole plate 4, the plate-shaped protrusions 3 are distributed in rows/columns, and the plate-shaped protrusions 3 in rows/columns can be mass produced only by simple procedures in the process of the production, thereby increasing the practicability of the device.

The shape of the plate-shaped protrusion 3 is the same as that of the deep hole plate 4, the plate-shaped protrusion 3 and the deep hole plate 4 are clamped together through friction force through the cooperation of the plate-shaped protrusion 3 and the groove 5 to form a whole, so that the plate-shaped protrusion 3 and the deep hole plate 4 are firmly connected, the connection stability is further guaranteed, the plate-shaped protrusion 3 can be further provided with a buckling position 31, the buckling position 31 is a notch formed in the plate-shaped protrusion 3, the groove 5 is internally provided with a joint position which is matched with the buckling position 31, each plate-shaped protrusion 3 is provided with a plurality of buckling positions 31, the contact area of the plate-shaped protrusion 3 and the deep hole plate 4 is increased through the arrangement of the buckling positions 31 and the joint position, and the friction force is increased, so that the plate-shaped protrusion 3 and the deep hole plate 4 are firmly connected.

In addition, the plate-like projections 3 may be formed in an aligned and combined shape for the efficiency of heating, so that the contact area between the plate-like projections 3 and the grooves 5 of the deep hole plate 4 can be increased to the maximum. Rows/columns of deep holes are formed above the deep hole plate 4, and grooves 5 are formed between each row and/or each column of deep holes. The shape of the groove 5 matches the shape of the plate-like protrusion 3.

Wherein the heat conduction seat 2 further comprises a side heating plate 21, and the side heating plate 21 is arranged at the side of the main heating plate 22 and is perpendicular to the extending direction of the plate-shaped protrusion 3; the side heating plate can assist in heating the hole sites on the side, and heat dissipation of the side holes is avoided.

Optionally, the main heating plate 22 is pre-buried in the bottom of the deep hole plate 4, and the main heating plate 22 and the deep hole plate 4 are integrally formed, so that the influence of heat transfer faults generated by too many heat conductors on the heating result can be avoided. Alternative embodiments may also place the main heating plate 22 at the bottom of the deep hole plate 4, which can facilitate mass production and increase practicality.

In the practical use process, the deep hole plate 4 is generally selected from a 48 hole plate and a 96 hole plate, the side walls of the row of deep holes of the 48 hole plate and the 96 hole plate are tightly attached to the side walls of the row of deep holes of the 96 hole plate by the aid of the plate-shaped protrusions 3, the distance between every two adjacent plate-shaped protrusions 3 can just accommodate a single row of deep holes of the 48 hole plate, and simultaneously can accommodate two rows of deep holes of the 96 hole plate, at the moment, only the bottom and one side edge of the single row of deep holes of the 96 hole plate are in contact with a main heating plate, but the required heating solution is small in volume, so that the requirement of rapid heating can be met.

In order to further improve the heat conduction performance of the heat conduction seat 2, the material of the heat conduction seat 2 is aluminum, aluminum metal has good heat conduction performance, the cost of the aluminum heat conduction seat 2 is low, the large-scale production and use are convenient, and the practicability of the device is improved.

As shown in fig. 5, the device is further provided with a thermal protection switch 6, the thermal protection switch 6 is arranged on one side of the heating element 1, and the thermal protection switch 6 is electrically connected with the heating element 1, when the heating element 1 is abnormal, the heating element 1 is controlled by the thermal protection switch 6 to stop heating when the heating element 1 is heated at too high temperature and is easy to be dangerous, so that the whole device is protected, and the safety of the whole device is improved.

The device is also provided with a temperature control sensor 7, the temperature control sensor 7 is arranged on one side of the heating element 1, the temperature control sensor 7 is used for controlling the temperature of the heating element 1, and the temperature of the heating element 1 can be detected and controlled through the temperature control sensor 7 because the required temperatures of different reagents are different, so that the requirements of different reagents are met, and the device has the advantages of being accurate and efficient through the control of the temperature control sensor 7, and the practicability of the device is improved.

The device is also provided with a heat shield 8, a heat insulation groove is arranged in the heat shield 8, the heating element 1 is placed in the heat insulation groove, the upper surface of the heating element 1 is directly connected with the heat conduction seat 2, and the heat conduction seat 2 is adaptively connected with the deep hole plate 4; the heat-conducting seat 2 and the heat-insulating cover 8 are generally made of materials with poor heat-conducting performance, the heat-insulating cover 8 can effectively isolate heat of the heating element 1 from overflowing, the heating speed of the heating element 1 is increased by phase change, the working efficiency of the whole device is increased, and the practicability of the device is improved.

The device is also provided with a base 9, the heat shield 8 is suitable for being placed on the base 9, the device can be placed on most of work tables through the base 9, and the base 9 is supported on the ground through four legs, so that the whole device is more stable.

Example 2

A nucleic acid extraction apparatus comprising the heating mechanism of the nucleic acid extraction apparatus of any one of embodiment 1.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the utility model.

Claims

1. A heating mechanism of a nucleic acid extractor, comprising:

a heating element (1);

the heat conduction seat (2) is connected with the heating element (1), the heat conduction seat (2) comprises a main heating plate (22), and the main heating plate (22) is provided with platy protrusions (3) distributed in rows at intervals;

the deep hole plate (4), be provided with recess (5) between every row of reaction hole of deep hole plate (4) bottom, recess (5) can with platy protrusion (3) closely laminate.

2. The heating mechanism of a nucleic acid isolation instrument according to claim 1, wherein the plate-like projection (3) is provided with a fastening position (31), and the deep well plate (4) is provided with a joint position.

3. The heating mechanism of a nucleic acid isolation instrument according to claim 2, wherein the heat conducting base (2) further comprises a side heating plate (21), and the side of the main heating plate (22) is provided with the side heating plate (21) perpendicular to the extending direction of the plate-like protrusions (3).

4. The heating mechanism of a nucleic acid isolation instrument according to claim 1, characterized in that the main heating plate (22) is detachably connected to the heating element (1).

5. The heating mechanism of the nucleic acid extractor according to claim 1, characterized in that the main heating plate (22) is pre-buried at the bottom of the deep hole plate (4), and the main heating plate (22) and the deep hole plate (4) are integrally formed.

6. The heating mechanism of a nucleic acid extracting apparatus as defined in claim 1, wherein the deep-hole plate (4) is a 48-well plate, and adjacent plate-like projections (3) are closely fitted to grooves (5) on both sides of each row of reaction wells of the 48-well plate.

7. The heating mechanism of a nucleic acid extractor according to claim 1, wherein the deep-hole plate (4) is a 96-well plate, and adjacent plate-like projections (3) are closely fitted to grooves (5) on both sides of each two rows of reaction wells of the 96-well plate.

8. The heating mechanism of the nucleic acid extractor according to claim 3, further comprising:

a heat shield (8) connected with the bottom of the heating element (1); the heat shield (8) is provided with a heat insulation groove, and the heating element (1) is arranged in the heat insulation groove.

9. The heating mechanism of the nucleic acid extractor of claim 8, further comprising:

-a base (9) connected to said heat shield (8), said base (9) being adapted to house said heat shield (8).

10. A nucleic acid extractor comprising the heating mechanism of the nucleic acid extractor of any one of claims 1 to 8.