CN218989286U

CN218989286U - Device for in-vitro cell expansion

Info

Publication number: CN218989286U
Application number: CN202223428848.3U
Authority: CN
Inventors: 郭天欢; 赵炳榛; 黄莹莹
Original assignee: Henan Guoxin Biotechnology Co ltd
Current assignee: Henan Guoxin Biotechnology Co ltd
Priority date: 2022-12-21
Filing date: 2022-12-21
Publication date: 2023-05-09
Anticipated expiration: 2032-12-21

Abstract

The utility model discloses a device for in-vitro cell expansion, and belongs to the technical field of extracellular expansion. The device for in-vitro cell expansion comprises a box body, wherein a placement position is arranged in the box body, a storage barrel is detachably arranged on the placement position, the storage barrel can be used for inserting the placement barrel, an ejection assembly used for ejecting the placement barrel is arranged in the storage barrel, a cover plate is detachably arranged on the box body, a placement channel used for enabling the placement barrel to be inserted is formed in the cover plate, and a locking piece used for fixing the placement barrel is arranged on the cover plate; the utility model solves the problems that various storage barrels cannot be replaced and the storage barrels are easy to pop up in the prior art.

Description

Device for in-vitro cell expansion

Technical Field

The utility model relates to the technical field of extracellular amplification, in particular to a device for in-vitro amplification of cells.

Background

Natural killer cells (natural killer cell, NK) are important immune cells of the body, not only associated with anti-tumor, anti-viral infection and immunomodulation, but also in some cases involved in the development of hypersensitivity reactions and autoimmune diseases, capable of recognizing target cells, killing mediators.

NK immune cells require the use of specialized kit aids during culture expansion, such as: the utility model discloses a novel in vitro amplification kit of NK immunocyte of china of application number 202122676806.0, this kit includes the box body, be provided with the receiving section of thick bamboo in the box body, be provided with the spring in the receiving section of thick bamboo, be provided with the slide on the spring, place the section of thick bamboo and insert at the receiving section of thick bamboo, be provided with the cell in the receiving section of thick bamboo, the opening part of receiving box is provided with sealed lid, sealed lid is fixed in the box body through the kelly, it inserts the intrinsic sealed lid that rotates of receiving section of thick bamboo to place the section of thick bamboo, the kelly just fixes in the box body like this, only need rotate sealed lid when taking out one of them, make the kelly will be perpendicular from the spout, the spring will be ejecting the receiving section of thick bamboo simultaneously, when such taking out or placing receiving section of thick bamboo (reagent pipe), avoid directly opening the whole lid of box body, be difficult for making other test tubes receive the pollution.

The whole lid of box body can be effectively avoided directly to foretell kit, is difficult for making other test tubes receive the pollution, but still has some problems in the in-process of using, 1: the storage barrel is fixed in the box body, so that the storage barrel is only applicable to one storage barrel and cannot be applicable to various storage barrels.

2: the box body is not provided with a device for fixing the clamping rod, the clamping rod is easy to move due to external interference in the use process, the clamping rod is vertical to the sliding groove, and the whole storage barrel is easy to eject by the spring.

Disclosure of Invention

The utility model aims to solve the problems that a plurality of storage barrels cannot be replaced and the storage barrels are easy to pop up in the prior art, and provides a device for in-vitro cell expansion.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a device for external expansion of cell, includes the box body, be provided with in the box body and place the position, demountable installation has a receiving vessel on the position of placing, the receiving vessel can supply to place the section of thick bamboo and insert, be provided with in the receiving vessel and be used for placing the ejecting subassembly of section of thick bamboo, box body demountable installation has the apron, set up on the apron and be used for making to place a section of thick bamboo male placement channel, be provided with on the apron and be used for carrying out fixed retaining member to placing the section of thick bamboo.

Further, be provided with the internal thread in the position of placing, the receiving cylinder includes the stack shell, installs the lead screw on the stack shell, the screw thread and the internal thread looks adaptation on lead screw surface, ejecting subassembly is installed in the stack shell, the stack shell upper end is for being used for receiving the stack shell to put into the open end.

Further, the ejection assembly comprises a spring and a top plate arranged on the spring, and one end, far away from the top plate, of the spring is arranged in the cylinder body.

Further, the placing cylinder comprises a reagent tube and a sealing cover arranged on the reagent tube, wherein the sealing cover comprises a cover body, supporting rods arranged on two sides of the cover body, and a screwing block arranged at the upper end of the cover body.

Further, a through hole for the supporting rod to be inserted is formed in the cover plate, a placing groove for the supporting rod to be placed in is formed in the cover plate, and the placing groove is the locking piece.

Further, the box body is provided with a groove, the cover plate is provided with a plugboard matched with the groove, the plugboard is provided with a threaded hole, the box body is provided with a unthreaded hole, and the threaded hole and the unthreaded hole are fixed through bolts.

Furthermore, an ultraviolet lamp is arranged on the inner side wall of the box body.

Further, handles are arranged on two sides of the box body.

Further, the box body is detachably provided with a protective cover.

Compared with the prior art, the utility model provides a device for in-vitro cell expansion, which has the following beneficial effects.

The device has the advantages that the parts which are not involved in the device are the same as or can be realized by adopting the prior art,

1: because the apron demountable installation is on the box body, and the receiving cylinder is demountable installation also on placing the position simultaneously, just so can dismantle the apron and change the receiving cylinder, when changing the receiving cylinder of equidimension not, still need to change corresponding apron, make place the passageway size and place the reagent pipe of a section of thick bamboo surface size unanimity, can adjust according to the equidimension like this, when a certain receiving cylinder is damaged, just can directly dismantle the apron change can.

2: the existing cover plate is provided with more than one placing groove than the original cover plate, the placing barrel is inserted into the containing barrel through the placing channel during use, the supporting rod is inserted into the placing groove by rotating the screwing block, the supporting rod is separated from the placing groove by screwing the screwing block during taking out, and then the screwing block is rotated, so that the through hole is aligned with the supporting rod, and the containing barrel is ejected out by the ejection assembly, so that the containing barrel can be taken out.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows; and will be apparent to those skilled in the art in part based upon a review of the following; alternatively, the teachings may be directed to practice of the present utility model.

Drawings

Fig. 1 is a perspective view of the present utility model.

Fig. 2 is a second perspective view of the present utility model (the protective cover is not shown).

Fig. 3 is a schematic view of the structure of the cover plate and the storage barrel of the utility model.

Fig. 4 is a schematic diagram of a cover plate structure according to the present utility model.

Fig. 5 is a schematic diagram of a cover plate structure according to the present utility model.

Fig. 6 is a schematic view of the structure of the cartridge of the present utility model.

Fig. 7 is a perspective view III of the present utility model (the protective cover, the cover plate and the storage barrel are not shown).

Fig. 8 is a cross-sectional view of fig. 7.

In the figure:

1. a case body; 2. a cover plate; 3. a barrel body; 4. a protective cover; 5. a through hole; 6. twisting the block;

7. a cover body; 8. a reagent tube; 9. inserting plate; 10. placing a channel; 11. a threaded hole; 12. a placement groove;

13. a support rod; 14. an ultraviolet lamp; 15. a groove; 16. a placement bit; 17. a spring;

18. a screw rod; 19. and a top plate.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1 to 8, a device for cell in-vitro amplification comprises a box body 1, wherein a placement position 16 is arranged in the box body 1, a storage barrel is detachably arranged on the placement position 16 and can be inserted by the placement barrel, an ejection assembly for ejecting the placement barrel is arranged in the storage barrel, a cover plate 2 is detachably arranged on the box body 1, a placement channel 10 for enabling the placement barrel to be inserted is formed in the cover plate 2, and a locking piece for fixing the placement barrel is arranged on the cover plate 2.

In this embodiment, the cover plate 2 can be removed to replace the storage barrel, and when the storage barrels with different sizes are replaced, the corresponding cover plate 2 is required to be replaced, so that the size of the placing channel 10 is consistent with the surface size of the placing barrel, and the reagent tube 8 with different sizes can be adjusted, and the outside air is used.

The storage position 16 is internally provided with internal threads, the storage barrel comprises a barrel body 3 and a screw rod 18 arranged on the barrel body 3, threads on the surface of the screw rod 18 are matched with the internal threads, the ejection assembly is arranged in the barrel body 3, and the upper end of the barrel body 3 is used for storing the storage barrel into an open end.

In this embodiment, the shaft 3 can be mounted on the placement position 16 through the screw 18, so that the accommodating barrels with different types can be better mounted or replaced, and meanwhile, the placement channel 10 can be used for gas circulation, which is beneficial to gas exchange of the culture bag in the cell culture process and cell growth and propagation.

The ejection assembly comprises a spring 17, a top plate 19 mounted on the spring 17, and one end of the spring 17 remote from the top plate 19 is mounted in the barrel 3.

In this embodiment, the spring 17 can eject the top plate 19 and simultaneously eject the cartridge, thus better removing the cartridge.

The placing cylinder comprises a reagent tube 8 and a sealing cover arranged on the reagent tube 8, wherein the sealing cover comprises a cover body 7, supporting rods 13 arranged on two sides of the cover body 7 and a screwing block 6 arranged at the upper end of the cover body 7, through holes 5 for the supporting rods 13 to be inserted are formed in the cover plate 2, placing grooves 12 for the supporting rods 13 to be placed in are formed in the cover plate 2, and the placing grooves 12 are locking pieces.

In this embodiment, when the placement barrel is placed, the placement barrel is only required to be inserted into the placement channel 10, then the cover body 7 is pressed, then the rotating block is rotated to align the support rod 13 with the placement groove 12, and when the rotating block is loosened, the spring 17 pushes the support rod 13 into the placement groove 12, so that the dislocation of the support rod 13 can be avoided.

The box body 1 is provided with a groove 15, the cover plate 2 is provided with a plugboard 9 matched with the groove 15, the plugboard 9 is provided with a threaded hole 11, the box body 1 is provided with a unthreaded hole, and the threaded hole 11 is fixed with the unthreaded hole through a bolt.

In this embodiment, the bolts fix the box body 1 and the plugboard 9, and the bolts can be designed to better remove the cover plate 2, and meanwhile, the springs 17 are prevented from ejecting the cover plate 2.

An ultraviolet lamp 14 is mounted on the inner side wall of the case 1.

In this embodiment, the ultraviolet lamp 14 has a sterilizing effect.

Handles are arranged on two sides of the box body 1, and a protective cover 4 is detachably arranged on the box body 1.

In this embodiment, the cover 4 is more beautiful, and the handle is easier to handle.

The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.

Claims

1. The utility model provides a device for external expansion of cell, includes box body (1), its characterized in that, be provided with in box body (1) and place position (16), detachably installs the receiving vessel on placing position (16), the receiving vessel can supply to place the section of thick bamboo and insert, be provided with in the receiving vessel and be used for placing the ejecting subassembly of section of thick bamboo, box body (1) detachably installs apron (2), offer on apron (2) and be used for making to place a section of thick bamboo male placement channel (10), be provided with on apron (2) and be used for carrying out fixed retaining member to placing a section of thick bamboo.

2. The device for cell in-vitro amplification according to claim 1, wherein the placement position (16) is internally provided with internal threads, the accommodating barrel comprises a barrel body (3) and a screw rod (18) arranged on the barrel body (3), threads on the surface of the screw rod (18) are matched with the internal threads, the ejection assembly is arranged in the barrel body (3), and the upper end of the barrel body (3) is an opening end for accommodating the barrel.

3. Device for the ex vivo expansion of cells according to claim 2, characterized in that said ejection assembly comprises a spring (17), a top plate (19) mounted on the spring (17), the end of the spring (17) remote from the top plate (19) being mounted inside the barrel (3).

4. A device for in vitro cell expansion according to claim 3, wherein the placing cylinder comprises a reagent tube (8), a sealing cover mounted on the reagent tube (8), the sealing cover comprises a cover body (7), supporting rods (13) arranged at two sides of the cover body (7), and a screwing block (6) mounted at the upper end of the cover body (7).

5. The device for in vitro cell expansion according to claim 4, wherein the cover plate (2) is provided with a through hole (5) for inserting the supporting rod (13), the cover plate (2) is provided with a placing groove (12) for placing the supporting rod (13), and the placing groove (12) is the locking piece.

6. The device for cell in-vitro amplification according to claim 1, wherein the box body (1) is provided with a groove (15), the cover plate (2) is provided with a plugboard (9) matched with the groove (15), the plugboard (9) is provided with a threaded hole (11), the box body (1) is provided with a light hole, and the threaded hole (11) and the light hole are fixed through bolts.

7. Device for the in vitro cell expansion according to claim 1, characterized in that the inside wall of the box (1) is fitted with an ultraviolet lamp (14).

8. Device for the in vitro expansion of cells according to claim 1, characterized in that the cartridge (1) is provided with handles on both sides.

9. Device for the in vitro expansion of cells according to claim 1, characterized in that said cartridge (1) is removably fitted with a protective cover (4).