CN218969249U

CN218969249U - Culture rack

Info

Publication number: CN218969249U
Application number: CN202223272238.9U
Authority: CN
Inventors: 熊祥; 彭松林; 瞿鹏; 谭威; 陈长胜
Original assignee: Wuhan Mutual United Technology Co ltd
Current assignee: Wuhan Mutual United Technology Co ltd
Priority date: 2022-12-07
Filing date: 2022-12-07
Publication date: 2023-05-05
Anticipated expiration: 2032-12-07

Abstract

The utility model discloses a culture rack, and relates to the technical field of in-vitro cell culture. The device comprises a three-dimensional frame, a plurality of horizontal culture layers and a plurality of independent culture cabins, wherein the plurality of horizontal culture layers are arranged in the three-dimensional frame in a stacked manner along the height direction, and each horizontal culture layer is uniformly provided with the plurality of independent culture cabins; the three-dimensional frame comprises a mounting bottom plate and two vertical frame plates which are oppositely arranged on two sides of the mounting bottom plate, a plurality of horizontal culture layers are arranged between the two vertical frame plates, a plurality of supporting pieces distributed along the height direction are arranged on each vertical frame plate, and two sides of each horizontal culture layer are correspondingly provided with supporting pieces to support the horizontal culture layers. The culture rack is provided with a plurality of horizontal culture layers, and each horizontal culture layer can be provided with a plurality of independent culture cabins, so that the capacity of the culture box is increased, and the large-scale culture cost is reduced.

Description

Culture rack

Technical Field

The utility model relates to the technical field of cell in-vitro culture, in particular to a culture rack.

Background

Assisted reproductive in vitro fertilization refers to the technique by which mammalian sperm and eggs are subjected to the fertilization process in an in vitro environment. After fertilization, fertilized eggs are further cultured in vitro in an incubator for 3-7 days, and the whole in vitro fertilization process is finished after transplantation, freezing and other operations are completed.

The existing in vitro culture of mammal and human embryo is realized by placing the culture dish containing fertilized ovum or embryo in a conventional carbon dioxide incubator, three-gas incubator or time difference incubator for culturing, and simultaneously ensuring the temperature, humidity and cleanliness of the culture environment.

The existing carbon dioxide incubator or three-gas incubator can generally be used for placing 6 or 10 or 15 culture dishes, if the number of embryos or fertilized eggs to be cultured and observed exceeds 15, the number of the incubators needs to be increased, and the selling price of a single incubator is as high as two million, so that the cost is increased by continuously adding new incubators, and the technical development burden is increased.

Disclosure of Invention

The utility model provides a culture rack, which is provided with a plurality of horizontal culture layers, and a plurality of independent culture cabins can be arranged in each horizontal culture layer, so that the capacity of an incubator is increased, and the large-scale culture cost is reduced.

The technical scheme for solving the problems is as follows: providing a culture rack, wherein the culture rack comprises a three-dimensional frame, a plurality of horizontal culture layers and a plurality of independent culture cabins, the plurality of horizontal culture layers are arranged in the three-dimensional frame in a stacking manner along the height direction, and each horizontal culture layer is uniformly provided with the plurality of independent culture cabins; the three-dimensional frame comprises a mounting bottom plate and two vertical frame plates which are oppositely arranged on two sides of the mounting bottom plate, a plurality of horizontal culture layers are arranged between the two vertical frame plates, a plurality of supporting pieces distributed along the height direction are arranged on each vertical frame plate, and two sides of each horizontal culture layer are correspondingly provided with supporting pieces to support the horizontal culture layers.

Further, the support piece is arranged to be a sliding rail, sliding seats are correspondingly arranged on two sides of the horizontal culture layer, and the sliding seats are arranged on the sliding rail in a sliding mode.

Further, the device also comprises a driving mechanism for driving the horizontal culture layers to move along the extending direction of the sliding track, and each horizontal culture layer is correspondingly driven to move by at least one driving mechanism.

Further, the actuating mechanism includes driving motor, conveyer belt, band pulley, follow-up piece, driving motor and band pulley set up support piece's both ends respectively, the conveyer belt transmission sets up between driving motor and band pulley, the follow-up piece is fixed to be set up on the conveyer belt, and the follow-up piece still with horizontal culture layer fixed connection.

Further, the mounting base plate is also provided with a driving controller capable of controlling one or more driving mechanisms to work and a driving power supply for supplying electric energy to the driving mechanisms.

Further, the independent culture cabin comprises a cabin cover and a containing cabin with an opening, wherein the containing cabin is fixedly arranged on the horizontal culture layer, and the cabin cover is used for sealing the opening of the containing cabin.

Further, at least one gland assembly is arranged above each horizontal culture layer and is used for tightly covering the cabin cover on the accommodating cabin.

Further, each gland assembly comprises a gland cross beam with two ends fixedly connected to the vertical frame plate respectively and gland rollers arranged on the gland cross beam at equal intervals, and the gland rollers are rotatably arranged on the gland cross beam.

Further, a plurality of independent culture cabin determinant is arranged on the horizontal culture layer, and the number of the gland rollers arranged on one gland cross beam is larger than or equal to the number of one row of independent culture cabins.

The utility model has the beneficial effects that:

1. the stacked arrangement of a plurality of horizontal culture layers is in the three-dimensional frame, and all be equipped with a plurality of independent culture cabins on every horizontal culture layer, all load the culture dish in every independent culture cabin, be equivalent to making a plurality of culture dishes arrange in layers, and the culture dish range upon range of arrangement of every layer distributes, finally forms the stacked culture rack structure of beehive to the quantity of the culture dish that single incubator can be held is increased to the very big degree, reduces incubator quantity, reduce cost.

2. Each culture dish is placed in an independent culture cabin, and besides the gas supply required by the normal development of the embryo or fertilized egg, the independent culture cabin can isolate the culture dish from the external environment so as to prevent the embryo or fertilized egg in the culture dish from being polluted by the external environment.

3. Although the horizontal culture layers are arranged in a plurality, and the spacing between the adjacent horizontal culture layers is smaller, each horizontal culture layer can be moved independently, and a technician can control the horizontal culture layers to move so that the target horizontal culture layer is staggered with other horizontal culture layers, thereby facilitating the technician to take and place the culture dishes in the independent culture tanks on the target horizontal culture layer.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description, serve to explain the principles of the utility model. In the drawings, like reference numerals are used to identify like elements. The drawings, which are included in the description, illustrate some, but not all embodiments of the utility model. Other figures can be derived from these figures by one of ordinary skill in the art without undue effort.

FIG. 1 is a view showing the whole construction of a culture rack according to the embodiment;

FIG. 2 is a schematic structural view of a laminated arrangement of a plurality of horizontal culture layers of the present embodiment;

FIG. 3 is a structural view of the independent culture tanks of the present embodiment;

FIG. 4 is an enlarged view of a portion of FIG. 1;

1-three-dimensional frames, 11-mounting bottom plates, 12-vertical frame plates, 13-supporting pieces, 2-horizontal culture layers, 21-sliding seats, 3-independent culture cabins, 31-cabins, 32-accommodating cabins, 4-driving mechanisms, 41-driving motors, 42-conveying belts, 43-belt pulleys, 44-follower blocks, 5-driving controllers, 6-driving power supplies, 7-gland assemblies, 71-gland beams and 72-gland rollers.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be arbitrarily combined with each other.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

Referring to fig. 1 to 4, a culture rack according to an embodiment of the present utility model includes a three-dimensional frame 1, a plurality of horizontal culture layers 2, and a plurality of independent culture chambers 3 capable of loading culture dishes, wherein the plurality of horizontal culture layers 2 are stacked in the three-dimensional frame 1 along a height direction, and each horizontal culture layer 2 is provided with a plurality of independent culture chambers 3; the three-dimensional frame 1 comprises a mounting bottom plate 11 and two vertical frame plates 12 which are oppositely arranged on two sides of the mounting bottom plate 11, a plurality of horizontal culture layers 2 are arranged between the two vertical frame plates 12, a plurality of supporting pieces 13 which are distributed along the height direction are arranged on each vertical frame plate 12, and two sides of each horizontal culture layer 2 are correspondingly provided with supporting pieces 13 to support the horizontal culture layers 2.

Specifically, referring to fig. 1, when the culture rack of the present embodiment is disposed in an incubator, the mounting baseplate 11 is positioned at the bottom of the incubator, two upright frame plates 12 are disposed at the left and right sides of the mounting baseplate 11 opposite to each other, and a plurality of supporting members 13 disposed at equal intervals in the height direction are disposed on each upright frame plate 12. The supporting pieces 13 on the left vertical frame plate 12 are opposite to the supporting pieces 13 deleted by the right vertical frame plate 12 in the height direction one by one, so that the supporting pieces 13 on the left vertical frame plate 12 and the supporting pieces 13 on the right vertical frame plate 12 at the same height support the horizontal culture layer 2 together; the horizontal culture layers 2 are supported by the left and right side supporting members 13 at each height position along the height direction, so that a plurality of horizontal culture layers 2 are distributed in a stacked manner to form a stacked culture rack for carrying a plurality of independent culture tanks 3 and culture dishes loaded in the independent culture tanks 3.

In the present embodiment, the support 13 is a bar, and the upright frame plate 12 to which the plurality of support 13 are mounted is similar to a grid plate.

In this embodiment, because the interval between adjacent horizontal culture layers 2 is smaller, in order to avoid the upper horizontal culture layer 2 from forming shielding to the lower horizontal culture layer 2, the technician takes and places the culture dish, the supporting piece 13 is set to the sliding track, the two sides of the horizontal culture layer 2 are correspondingly provided with the sliding seat 21, and the sliding seat 21 is slidably arranged on the sliding track, so that each horizontal culture layer 2 can move. The movement of the horizontal culture layer 2 is controlled by a driving mechanism 4, the driving mechanism 4 comprises a driving motor 41, a conveyor belt 42, a belt wheel 43 and a follow-up block 44, the driving motor 41 and the belt wheel 43 are respectively arranged at two ends of the supporting piece 13, the conveyor belt 42 is arranged between the driving motor 41 and the belt wheel 43 in a transmission way, the follow-up block 44 is fixedly arranged on the conveyor belt, and the follow-up block 44 is fixedly connected with the horizontal culture layer 2.

Specifically, when the supporting member 13 is configured as a sliding rail, the supporting member 13 is laterally arranged, and the sliding seat 21 is correspondingly and fixedly installed at the side wall position of the horizontal culture layer 2, so that the supporting member 13 supports the horizontal culture layer 2 at the side wall thereof. Meanwhile, the sliding seat 21 is provided with a sliding connection groove, the cross section of the sliding connection groove is consistent with the cross section of the supporting piece 13 in size, so that the sliding seat 21 is in sliding clamping connection with the supporting piece 13, and the supporting piece 13 can support the horizontal culture layer 2 and further can meet the sliding requirement of the horizontal culture layer 2 along the extending direction of the supporting piece 13. Moreover, when a certain horizontal culture layer 2 moves horizontally, under the cooperation limiting effect of the sliding seat 21 and the supporting piece 13, the stability of the horizontal movement of the horizontal culture layer 2 is also high, and the phenomena of shaking, blocking and the like when the horizontal culture layer 2 moves are avoided.

The driving motor 41 works to drive the conveyor belt 42 to transport and move the follower block 44, and the follower block 44 makes linear motion between the driving motor 41 and the belt wheel 43, so that the horizontal culture layer 2 is driven to move.

Moreover, the conveyor belt 42 and the supporting member 13 are parallel to each other, so that the driving mechanism 4 can further ensure that the horizontal culture layer 2 can be stably moved.

In this embodiment, a driving mechanism 4 drives a horizontal culture layer 2 horizontally. In the example shown in fig. 1, a driving mechanism 4 is disposed above each support member 13 on the right upright frame, and the driving mechanism 4 is used for driving the horizontal culture layer 2 correspondingly supported by the support member 13 to move.

In the present embodiment, the driving motor 41 is preferably a servo motor that can simultaneously rotate forward and backward.

In the present embodiment, the horizontal culture layer 2 is allowed to move, and the purpose of providing the plurality of driving mechanisms 4 to control the automatic movement of the horizontal culture layer 2, respectively, is to: when the culture dishes are taken and placed, the plurality of horizontal culture layers 2 are staggered with the target horizontal culture layer 2, so that technicians can conveniently take the culture dishes in the corresponding independent culture tanks 3 on the target horizontal culture layer 2 or put the culture dishes in the independent culture tanks 3 on the target horizontal culture layer 2.

In this regard, the mounting base 11 is provided with a drive controller 5 for controlling the operation of one or more drive mechanisms 4, and a drive power supply 6 for supplying power to the plurality of drive mechanisms 4.

Specifically, when it is necessary to remove the culture dishes loaded on a certain horizontal culture layer 2, a technician may control the operation of the corresponding driving mechanism 4 through the driving controller 5 so that the target horizontal culture layer 2 alone moves to a position completely staggered from other horizontal culture layers 2. At this time, the upper side of the horizontal culture layer 2 is not shielded, and a technician can easily take the culture dish from the independent culture compartment 3 thereon or put the culture dish into the empty independent culture compartment 3.

It should be noted that, the driving controller 5 is a selection controller, such as a PLC controller, and the driving power supply 6 may be connected to an external power supply or a standby power supply also mounted on the mounting board 11 to supply power to the driving mechanism 4.

In the present embodiment, the independent culturing compartment 3 includes a compartment cover 31 and a housing compartment 32 having an opening, the housing compartment 32 is fixedly provided on the horizontal culturing layer 2, and the compartment cover 31 is used for sealing the opening of the housing compartment 32.

Specifically, the accommodation chamber 32 is provided in the middle of the upper surface of the horizontal culture layer 2, and the hatch 31 is detachably mounted to the accommodation chamber 32 and is used for sealing the upper opening of the accommodation chamber 32. When the hatch 31 is closed over the opening of the accommodation compartment 32, the independent culture compartment 3 is only in communication with the air supply device for supplying air to the embryo or fertilized egg.

In this embodiment, at least one capping assembly 7 is disposed above each horizontal culture layer 2, and the capping assembly 7 is used to press and cap the hatch 31 onto the accommodating chamber 32. Each gland assembly 7 comprises a gland cross beam 71 with two ends respectively fixedly connected to the vertical frame plate 12, and gland rollers 72 equidistantly arranged on the gland cross beam 71, wherein the gland rollers 72 are rotatably arranged on the gland cross beam 71. A plurality of independent culture tanks 3 are arranged on the horizontal culture layer 2 in a determinant, and the number of the gland rollers 72 arranged on one gland cross beam 71 is greater than or equal to the number of one row of independent culture tanks 3.

Specifically, when the operation of taking and placing the embryo culture dish is completed, the horizontal culture layer 2 moves back to the initial position, and in the process of returning the horizontal culture layer 2 to the initial position, the upper end surfaces of all the hatches 31 correspondingly contact with the gland rollers 72 above the moving path of the horizontal culture layer 2 in the moving process, and the gland rollers 72 contact with the hatches 31 and compress the hatches 31 on the accommodating cabin 32, so that some independent culture cabins 3 are prevented from being sealed tightly, and further gas components in the independent culture cabins 3 are prevented from leaking.

The above is applicable to the prior art, where nothing is mentioned.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting. Although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.

Claims

1. The culture rack comprises a three-dimensional frame (1), a plurality of horizontal culture layers (2) and a plurality of independent culture cabins (3) capable of loading culture dishes, wherein the plurality of horizontal culture layers (2) are arranged in the three-dimensional frame (1) in a stacking manner along the height direction, and the plurality of independent culture cabins (3) are uniformly distributed on each horizontal culture layer (2); the three-dimensional frame (1) is characterized by comprising a mounting bottom plate (11) and two vertical frame plates (12) which are oppositely arranged on two sides of the mounting bottom plate (11), wherein a plurality of horizontal culture layers (2) are arranged between the two vertical frame plates (12), a plurality of supporting pieces (13) distributed along the height direction are arranged on each vertical frame plate (12), and the two sides of each horizontal culture layer (2) are correspondingly provided with the supporting pieces (13) to support the horizontal culture layers (2).

2. A culture rack according to claim 1, characterized in that the support (13) is provided as a sliding rail, and that sliding seats (21) are provided on both sides of the horizontal culture layer (2), said sliding seats (21) being slidably arranged on the sliding rail.

3. A culture rack according to claim 2, further comprising a driving mechanism (4) for driving the horizontal culture layers (2) to move along the extending direction of the sliding track, wherein each horizontal culture layer (2) is correspondingly driven to move by at least one driving mechanism (4).

4. A culture rack according to claim 3, characterized in that the driving mechanism (4) comprises a driving motor (41), a conveyor belt (42), a belt wheel (43) and a follow-up block (44), wherein the driving motor (41) and the belt wheel (43) are respectively provided with two ends of the supporting piece (13), the conveyor belt (42) is arranged between the driving motor (41) and the belt wheel (43) in a transmission manner, the follow-up block (44) is fixedly arranged on the conveyor belt, and the follow-up block (44) is fixedly connected with the horizontal culture layer (2).

5. A culture rack according to claim 3, characterized in that the mounting base plate (11) is further provided with a drive controller (5) for controlling the operation of one or more drive mechanisms (4), and a drive power supply (6) for supplying electric power to the plurality of drive mechanisms (4).

6. A culture rack according to claim 1, characterized in that the independent culture compartment (3) comprises a compartment cover (31) and a containing compartment (32) with an opening, the containing compartment (32) being fixedly arranged on the horizontal culture layer (2), the compartment cover (31) being used for sealing the opening of the containing compartment (32).

7. A culture rack according to claim 6, wherein at least one capping assembly (7) is arranged above each horizontal culture layer (2), said capping assembly (7) being adapted to press-fit said hatch (31) onto the accommodation compartment (32).

8. A culture rack according to claim 7, wherein each gland assembly (7) comprises a gland cross beam (71) fixedly connected at both ends to the upright frame plate (12), respectively, gland rollers (72) equidistantly arranged on the gland cross beam (71), the gland rollers (72) being rotatably arranged on the gland cross beam (71).

9. A culture rack according to claim 8, wherein a plurality of said individual culture compartments (3) are arranged in rows on the horizontal culture layer (2), and the number of said capping rollers (72) arranged on one of said capping beams (71) is greater than or equal to the number of a row of individual culture compartments (3).