CN218951291U - Device for enzymolysis and desalination of protein sample - Google Patents
Device for enzymolysis and desalination of protein sample Download PDFInfo
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- CN218951291U CN218951291U CN202221880200.7U CN202221880200U CN218951291U CN 218951291 U CN218951291 U CN 218951291U CN 202221880200 U CN202221880200 U CN 202221880200U CN 218951291 U CN218951291 U CN 218951291U
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Abstract
The utility model belongs to the technical field of desalination devices, and particularly relates to a device for enzymolysis and desalination of a protein sample, which comprises a device main body, wherein a liquid outlet pipeline is arranged at the bottom end of the device main body, a control valve is arranged at the bottom end of the liquid outlet pipeline, a sealing cover plate is arranged at the top end of the device main body, an inner pipe body is arranged inside the device main body, an outer pipe body is arranged outside the device main body, a filtering assembly is arranged inside the inner pipe body, a piston rod is arranged at the top end of the sealing cover plate, a compression piston is arranged at the bottom end of the piston rod, and a liquid storage bin is arranged inside the inner pipe body. The utility model can achieve the purposes of convenient and quick connection for replacing the internal filter material and convenient use by arranging the compression piston, the filter assembly and the connecting groove.
Description
Technical Field
The utility model relates to the technical field of desalination devices, in particular to a device for enzymolysis desalination of a protein sample.
Background
The protein and peptide fragments are doped with more salt substances in the production process, so that desalination and purification of the protein are needed in laboratory researches, and the purpose is to isolate other salt small molecules in the protein and peptide fragments. Protein desalting is a method for purifying proteins, and currently common desalting methods include dialysis, electrodialysis and gel filtration. Dialysis and electrodialysis are long in time consumption, sample sparseness is large, large-scale production is not easy to amplify, and gel filtration realizes desalination by chromatographic flow of salt molecules of small molecules in protein solution at any time through size difference of salt molecules and protein molecules.
The prior art has the following problems:
the core part of the existing device for carrying out enzymolysis desalination on protein samples is a filter material placed in a desalination column, and the filter material is generally a disposable product and needs to be replaced after being used. The filter material of the existing desalting column is usually fixed into the desalting column in a plugging manner, and the desalting column is inconvenient to replace.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides a device for enzymolysis and desalination of a protein sample, which solves the existing problems.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a device for protein sample enzymolysis desalination, includes the device main part, and device main part bottom is provided with the drain pipe, and the drain pipe bottom is provided with control valve, and device main part top is provided with sealed apron, and the inside interior body that is provided with of device main part, the device main part outside are provided with outer body, and the internal side of interior is provided with filtering component, and sealed apron top is provided with the piston rod, and the piston rod bottom is provided with the compression piston, and the internal inside stock solution storehouse that is provided with of interior body.
As the preferable technical scheme of the utility model, the outer diameter of the inner pipe body is matched with the inner diameter of the outer pipe body, and the outer pipe body is movably connected with the inner pipe body.
As the preferable technical scheme of the utility model, the sealing cover plate is clamped with the top end of the inner pipe body, and sealing rings are arranged on the outer side of the sealing cover plate and the inner side of the top end of the outer pipe body.
As a preferable technical scheme of the utility model, an outer frame is arranged on the outer side of the filter assembly, a filter screen plate is arranged on the inner side of the filter assembly, a filler is arranged at the bottom end of the filter screen plate, and a connecting groove is arranged at the top end of the outer frame.
As the preferable technical scheme of the utility model, the top end of the outer frame is provided with a connecting groove, the bottom end of the inner pipe body is provided with an adaptive connecting block, and the inner pipe body is fixedly connected with the connecting block through a filtering component.
As the preferable technical scheme of the utility model, the piston rod is movably connected with the sealing cover plate, and the compression piston is movably connected with the inner pipe body through the piston rod.
Compared with the prior art, the utility model provides a device for enzymolysis and desalination of protein samples, which has the following beneficial effects:
according to the device for enzymolysis and desalination of the protein sample, the outer diameter of the inner pipe body is matched with the inner diameter of the outer pipe body, the outer pipe body is movably connected with the inner pipe body, and the inner pipe body can move in the outer pipe body, so that the device is convenient to detach; the sealing cover plate is clamped with the top end of the inner pipe body, and sealing rings are arranged on the outer side of the sealing cover plate and the inner side of the top end of the outer pipe body, so that the sealing performance of the device structure is improved; the outer frame is arranged on the outer side of the filter assembly, the filter screen plate is arranged on the inner side of the filter assembly, the filler is arranged at the bottom end of the filter screen plate, and the filter assembly is arranged in a modularized mode, so that the filter assembly is convenient to replace; the inner pipe body is fixedly connected with the connecting block through the filtering component, so that the filtering component is convenient to install and detach.
Drawings
FIG. 1 is a schematic diagram of a front view structure of the present utility model;
FIG. 2 is a schematic diagram of the internal structure of the front view of the present utility model;
FIG. 3 is a schematic view of the partial structure of FIG. 2 according to the present utility model.
In the figure: 1. a device body; 2. a liquid outlet pipe; 3. a control valve; 4. sealing the cover plate; 401. a seal ring; 5. an inner tube body; 501. a connecting block; 6. an outer tube body; 7. a filter assembly; 701. an outer frame; 702. filtering the sieve plate; 703. a filler; 704. a connecting groove; 8. a piston rod; 9. a compression piston; 10. and a liquid storage bin.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1 to 3, in the present embodiment: the utility model provides a device for protein sample enzymolysis desalination, including device main part 1, device main part 1 bottom is provided with liquid pipeline 2, liquid pipeline 2 bottom is provided with control valve 3 for the discharge of control liquid, device main part 1 top is provided with sealed apron 4, be used for the sealed at top, prevent external pollution, device main part 1 inside is provided with interior body 5, device main part 1 outside is provided with outer body 6, interior body 5 inboard is provided with filter element 7, be used for the filtration of desalination, sealed apron 4 top is provided with piston rod 8, be used for promoting compression piston 9, piston rod 8 bottom is provided with compression piston 9, interior body 5 inside is provided with stock solution storehouse 10.
In the embodiment, the outer diameter of the inner pipe body 5 is matched with the inner diameter of the outer pipe body 6, the outer pipe body 6 is movably connected with the inner pipe body 5, and thus the inner pipe body 5 can move in the outer pipe body 6, and the disassembly is convenient; the sealing cover plate 4 is clamped with the top end of the inner pipe body 5, and sealing rings 401 are arranged on the outer side of the sealing cover plate 4 and the inner side of the top end of the outer pipe body 6, so that the sealing performance of the device structure is improved; the outer frame 701 is arranged on the outer side of the filter assembly 7, the filter screen plate 702 is arranged on the inner side of the filter assembly 7, and the filler 703 is arranged at the bottom end of the filter screen plate 702, so that the desalting effect is good, and meanwhile, the filter assembly 7 is arranged in a modularized manner, so that the replacement is convenient; the top end of the outer frame 701 is provided with a connecting groove 704, the bottom end of the inner pipe body 5 is provided with an adaptive connecting block 501, the inner pipe body 5 is fixedly connected with the connecting block 501 through the filter assembly 7, and thus the installation and the disassembly of the filter assembly 7 are convenient; the piston rod 8 is movably connected with the sealing cover plate 4, and the compression piston 9 is movably connected with the inner pipe body 5 through the piston rod 8, so that the compression piston 9 is pushed by the piston rod 8 to drive the internal liquid to compress, and desalination is performed.
The working principle and the using flow of the utility model are as follows: when the desalting device is used, protein sample enzyme is placed in the liquid storage bin 10, the compression piston 9 is pushed by the piston rod 8 to drive the internal liquid to compress, so that desalting is performed, the external diameter of the inner pipe body 5 is matched with the internal diameter of the outer pipe body 6, the outer pipe body 6 is movably connected with the inner pipe body 5, and the inner pipe body 5 can move in the outer pipe body 6, so that the desalting device is convenient to detach; the sealing cover plate 4 is clamped with the top end of the inner pipe body 5, and sealing rings 401 are arranged on the outer side of the sealing cover plate 4 and the inner side of the top end of the outer pipe body 6, so that the sealing performance of the device structure is improved; the outer frame 701 is arranged on the outer side of the filter assembly 7, the filter screen plate 702 is arranged on the inner side of the filter assembly 7, the filler 703 is arranged at the bottom end of the filter screen plate 702, and the filter assembly 7 is arranged in a modularized manner so as to be convenient for replacement while the desalination effect is good; the inner pipe body 5 and the connecting block 501 are fixedly connected through the filter assembly 7, so that the filter assembly 7 is convenient to install and detach.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (6)
1. A device for enzymatic desalting of a protein sample, comprising a device body (1), characterized in that: the device is characterized in that a liquid outlet pipeline (2) is arranged at the bottom end of the device main body (1), a control valve (3) is arranged at the bottom end of the liquid outlet pipeline (2), a sealing cover plate (4) is arranged at the top end of the device main body (1), an inner pipe body (5) is arranged inside the device main body (1), an outer pipe body (6) is arranged outside the device main body (1), a filtering component (7) is arranged on the inner side of the inner pipe body (5), a piston rod (8) is arranged at the top end of the sealing cover plate (4), a compression piston (9) is arranged at the bottom end of the piston rod (8), and a liquid storage bin (10) is arranged inside the inner pipe body (5).
2. An apparatus for enzymatic desalting of a protein sample according to claim 1, wherein: the outer diameter of the inner pipe body (5) is matched with the inner diameter of the outer pipe body (6), and the outer pipe body (6) is movably connected with the inner pipe body (5).
3. An apparatus for enzymatic desalting of a protein sample according to claim 1, wherein: the sealing cover plate (4) is clamped with the top end of the inner pipe body (5), and sealing rings (401) are arranged on the outer side of the sealing cover plate (4) and the inner side of the top end of the outer pipe body (6).
4. An apparatus for enzymatic desalting of a protein sample according to claim 1, wherein: the filter comprises a filter assembly (7), and is characterized in that an outer frame (701) is arranged outside the filter assembly (7), a filter screen plate (702) is arranged on the inner side of the filter assembly (7), a filler (703) is arranged at the bottom end of the filter screen plate (702), and a connecting groove (704) is formed in the top end of the outer frame (701).
5. The apparatus for enzymatic desalting of a protein sample according to claim 4, wherein: the outer frame (701) top is provided with spread groove (704), interior body (5) bottom is provided with looks adaptation connecting block (501), interior body (5) and connecting block (501) pass through filtering component (7) fixed connection.
6. An apparatus for enzymatic desalting of a protein sample according to claim 1, wherein: the piston rod (8) is movably connected with the sealing cover plate (4), and the compression piston (9) is movably connected with the inner pipe body (5) through the piston rod (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221880200.7U CN218951291U (en) | 2022-07-20 | 2022-07-20 | Device for enzymolysis and desalination of protein sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221880200.7U CN218951291U (en) | 2022-07-20 | 2022-07-20 | Device for enzymolysis and desalination of protein sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218951291U true CN218951291U (en) | 2023-05-02 |
Family
ID=86138807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221880200.7U Active CN218951291U (en) | 2022-07-20 | 2022-07-20 | Device for enzymolysis and desalination of protein sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218951291U (en) |
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2022
- 2022-07-20 CN CN202221880200.7U patent/CN218951291U/en active Active
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