CN218795985U - Microfluidic chip for quantitative sampling - Google Patents

Abstract

The utility model relates to the technical field of quantitative sampling, in particular to a micro-fluidic chip for quantitative sampling, which comprises a rotary table and a quantitative disc, wherein the rotary table is embedded in the quantitative disc, and the rotary table rotates relative to the quantitative disc; a flow channel for sample introduction storage and quantitative sampling of sample liquid to be detected is arranged on the quantitative disc; set up the gating passageway on the carousel, through the rotation of carousel, realize that the circulation passageway on the ration dish switches between advancing a kind memory state and quantitative sample state, the utility model discloses the chip has not only realized the high accuracy ration sample of appearance liquid, and its structure is simpler, and whole body size is less, and is easily integrated, has avoided changing inconvenient and the higher problem of cost after the device vulnerable part damages simultaneously, has effectively prolonged the life of chip.

Description

Microfluidic chip for quantitative sampling

Technical Field

The utility model relates to a quantitative sampling technical field specifically is a micro-fluidic chip for quantitative sampling.

Background

The micro-fluidic chip is a hotspot field of the current development of micro total analysis systems, and aims to integrate the functions of the whole laboratory, including sampling, dilution, reagent addition, reaction, separation, detection and the like, on the micro-chip, and can be used for multiple times.

When the micro-fluidic chip samples, quantitative sampling needs to be carried out by matching with a six-way valve and a quantitative ring, and a six-way valve sample injector is an ideal sample injector in a high-efficiency liquid chromatography system and consists of a circular sealing pad and a fixed base. Working principle of the six-way valve sample injector: when the handle is positioned at the sampling position, the sample is injected into the quantitative ring from the sample inlet through the micro sample injection needle, and after the quantitative ring is filled, the redundant sample is discharged from the emptying hole; when the handle is rotated to the sample introduction position, the valve is communicated with the liquid phase flow path, the mobile phase conveyed by the pump washes the quantitative ring, and the sample is pushed to enter the liquid phase analysis column for analysis.

Although the existing sample introduction scheme can realize high-precision sample liquid measurement and is also convenient to use, the problem still exists: the six-way valve sample injector has a complex structure and large volume, is not easy to integrate in a microfluidic system, and is not beneficial to the miniaturization, portability and automation of the microfluidic system; when the handle of the six-way valve sample injector is positioned between the sampling position and the sample injection position, the flow path is blocked temporarily, the pressure in the flow path is increased suddenly and then the six-way valve sample injector is rotated to the sample injection position, and the column head is damaged easily due to overhigh pressure, so that the conventional six-way valve has short service life, high maintenance frequency and high replacement cost.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micro-fluidic chip for ration sample to provide micro-fluidic chip in solving above-mentioned background art when advancing the appearance, need cooperate six-way valve and ration ring to carry out the ration sample, make micro-fluidic system be not convenient for miniaturation, portable and the problem that integrates.

In order to achieve the above object, the utility model provides a following technical scheme:

a micro-fluidic chip for quantitative sampling comprises a rotary disc and a quantitative disc, wherein the rotary disc is embedded in the quantitative disc, and the rotary disc rotates relative to the quantitative disc;

a circulating channel for sample introduction storage and quantitative sampling of the sample liquid to be detected is arranged on the quantitative disc;

the rotary disc is provided with a gating channel, and the gating channel realizes the switching of the circulation channel on the quantitative disc between a sample storage state and a quantitative sampling state through the rotation of the rotary disc.

Furthermore, the circulation channel comprises a quantitative channel, one end of the quantitative channel is a sample introduction end, the sample introduction end of the quantitative channel is provided with a first interface communicated with the outside, and the first interface is communicated with the driving pump; the other end of the quantitative channel is a sampling end, the sampling end of the quantitative channel is provided with a second interface communicated with the outside, and the second interface is communicated with a sampling pump;

the flow channel also comprises a liquid storage channel, one end of the liquid storage channel is provided with a liquid inlet communicated with external liquid storage equipment, and the other end of the liquid storage channel is selectively communicated with the sample introduction end of the quantitative channel through a gating channel;

during sample injection, the liquid storage channel is communicated with the sample injection end of the quantitative channel through the gating channel, the sampling end of the quantitative channel is vacant and is not communicated, and the circulation channel is in a sample injection storage state; or, the rotating disc is rotated, the liquid storage channel, the quantitative channel and the gating channel are all vacant and are not communicated, and the flow channel is in a quantitative sampling state at the moment.

Furthermore, the circulation channel also comprises a waste liquid channel, one end of the waste liquid channel is provided with a liquid overflow port communicated with the outside, and the other end of the waste liquid channel is selectively communicated with the sampling end of the quantitative channel through a gating channel;

the two gating channels are respectively a first gating channel and a second gating channel, the first gating channel and the second gating channel are arc-shaped channels with equal volumes, and the first gating channel and the second gating channel are oppositely arranged on the turntable;

the liquid storage channel is communicated with the sample introduction end of the quantitative channel through the first gating channel, the sampling end of the quantitative channel is communicated with the waste liquid channel through the second gating channel, and the circulation channel is in a sample introduction storage state; or the rotating disc is rotated, the gating channel II is communicated with the sampling end of the quantitative channel, the sampling end of the quantitative channel is communicated with the gating channel I, the liquid storage channel and the waste liquid channel are both idle and are not communicated, and the circulating channel is in a quantitative sampling state at the moment.

Furthermore, the circulation channel also comprises a waste liquid channel, one end of the waste liquid channel is provided with a liquid overflow port communicated with the outside, and the other end of the waste liquid channel is selectively communicated with the sampling end of the quantitative channel through a gating channel;

the gating channels are three and respectively comprise a first gating channel, a second gating channel and a third gating channel, the first gating channel, the second gating channel and the third gating channel are three arc-shaped channels with equal volumes, and the first gating channel, the second gating channel and the third gating channel are uniformly distributed on the turntable;

the liquid storage channel is communicated with the sample introduction end of the quantitative channel through the first gating channel, the sampling end of the quantitative channel is communicated with the waste liquid channel through the second gating channel, the third gating channel is idle and is not communicated, and the circulation channel is in a sample introduction storage state; or the rotating disc is rotated, the gating channel III is communicated with the sample introduction end of the quantitative channel, the sampling end of the quantitative channel is communicated with the gating channel II, the liquid storage channel, the waste liquid channel and the gating channel I are all vacant and are not communicated, and the circulation channel is in a quantitative sampling state.

Further, the quantitative disc comprises a quantitative disc substrate and a quantitative disc cover plate, the quantitative disc cover plate is bonded on the quantitative disc substrate, and the flow channel is a cavity between a liquid flow groove body arranged on the quantitative disc substrate and the quantitative disc cover plate;

the rotary table comprises a rotary table substrate and a rotary table cover plate, the rotary table cover plate is bonded on the rotary table substrate, and the gating channel is a cavity between a liquid flow groove body arranged on the quantitative disc substrate and the quantitative disc;

the turntable is integrally embedded in the quantitative disc, the quantitative disc substrate corresponds to the turntable substrate in position and height, and the quantitative disc cover plate corresponds to the turntable cover plate in position and height; the circulation channel and the gating channel realize the switching of the circulation channel between a sample storage state and a quantitative sampling state through the rotation of the turntable;

the liquid inlet and the overflow port are arranged on the surface of the quantitative disc cover plate, the liquid storage channel is communicated with the outside through the liquid inlet, and the waste liquid channel is communicated with the outside through the overflow port; the interface I and the interface II are arranged on the surface of the cover plate of the quantitative disc, the sampling end of the quantitative channel is communicated with the outside through the interface I, and the quantitative channel is communicated with the sampling pump through the interface II.

Further, the volume of the quantitative channel is 3-150 muL, and the volume of the liquid storage channel is larger than that of the quantitative channel.

Furthermore, the port communication position of the circulation channel on the quantitative disc and the port communication position of the gating channel on the rotary disc are both labyrinth seal structures.

Furthermore, a limiting assembly is arranged between the rotary disc and the quantitative disc, the limiting assembly comprises a spring, a positioning steel ball, a sample feeding positioning groove and a sampling positioning groove, the quantitative disc is provided with a positioning hole, the spring and the positioning steel ball are arranged in the positioning hole, the spring is connected with the bottom of the positioning hole, and the positioning steel ball is arranged at the top of the spring; advance a kind constant head tank and sample constant head tank and set up on the carousel, advance a kind constant head tank and sample constant head tank and all be the hemisphere, location steel ball block is advancing a kind constant head tank or sample constant head tank in, location steel ball block is advancing a kind constant head tank in, and the circulation passageway is in a kind memory state, perhaps, the rotary table, location steel ball block is in sample constant head tank, and the circulation passageway is in the quantitative sample state.

Further, the border of carousel apron sets up supplementary appearance constant head tank and supplementary sample constant head tank, advance appearance constant head tank and sample constant head tank and set up on the carousel substrate, supplementary appearance constant head tank equals with advance the appearance constant head tank size, and the position is corresponding, supplementary sample constant head tank equals with the sample constant head tank size, and the position is corresponding, advance the appearance constant head tank and supplementary appearance constant head tank and constitute whole hemispherical groove that is, supplementary sample constant head tank and sample constant head tank are constituteed whole hemispherical groove.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) The utility model discloses the chip sets up relative pivoted carousel and ration dish, sets up quantitative passageway on the ration dish, sets up a plurality of gating passageways on the carousel, and the rotatory channel gating on quantitative passageway and the carousel to make quantitative passageway realize that the chip advances the switching of appearance storage state and quantitative sampling state, and then realized the high accuracy ration sample of appearance liquid, compare in the quantitative sampling device of current micro-fluidic, the utility model discloses the chip makes extremely thin and transparent ration sample chip based on the principle of six-way valve, and whole body volume is less, easily integration.

(2) The utility model discloses carousel and batch pan intercommunication department adopt labyrinth seal structure, when having avoided six-way valve sample injector handle to be in the sampling position and having advanced between the appearance position, because the rotation produces the problem that too high pressure arouses the column cap to damage, overcome current six-way valve life weak point, maintain the drawback that the maintenance frequency is high, effectively prolonged the life of chip, simple structure is convenient for change vulnerable part moreover, and the replacement cost is low.

Drawings

FIG. 1 is a schematic view of a sample storage state structure according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a quantitative sampling state according to a first embodiment of the present invention;

fig. 3 is a schematic structural view of a quantitative disc substrate according to a first embodiment of the present invention;

fig. 4 is a schematic view of a cover plate structure of a quantitative tray according to a first embodiment of the present invention;

fig. 5 is a schematic structural view of a substrate of a turntable according to a first embodiment of the present invention;

fig. 6 is a schematic diagram of a turntable cover plate structure according to a first embodiment of the present invention;

FIG. 7 is a schematic view of the labyrinth seal structure of the present invention;

FIG. 8 is a schematic view of a sample storage status structure according to a second embodiment of the present invention;

fig. 9 is a schematic structural view of a quantitative sampling state according to a second embodiment of the present invention;

fig. 10 is a schematic view of a quantitative sampling state structure according to a third embodiment of the present invention;

fig. 11 is a schematic structural view of a quantitative sampling state according to a fourth embodiment of the present invention;

fig. 12 is a schematic structural view of a quantitative sampling state according to a fifth embodiment of the present invention.

In the figure: 1. a liquid inlet; 2. an overflow port; 3. an interface I; 4. a second interface; 5. a liquid storage channel; 6. a waste liquid channel; 7. a dosing channel; 8. gating a first channel; 9. a second gating channel; 10. a gating channel III; 11. a spring; 12. positioning the steel balls; 13. a sample injection positioning groove; 14. a sampling positioning groove, 131 and an auxiliary sample introduction positioning groove; 141. an auxiliary sampling positioning groove; 15. a labyrinth seal structure; I. a recessed portion.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

The first embodiment is as follows:

referring to fig. 1 to 6, the present invention provides a microfluidic chip for quantitative sampling: the quantitative disc comprises a quantitative disc substrate and a quantitative disc cover plate, wherein a liquid circulation groove body used for sample introduction storage and quantitative sampling of a sample liquid to be detected is arranged on the quantitative disc substrate, the quantitative disc cover plate is bonded on the quantitative disc substrate, and a cavity formed by the liquid circulation groove body and the quantitative disc cover plate is a circulation channel; the turntable comprises a turntable substrate and a turntable cover plate, a plurality of liquid circulation groove bodies are formed in the turntable substrate, the turntable cover plate is bonded on the turntable substrate, and a cavity formed between the turntable cover plate and the liquid circulation groove bodies is a gating channel; the whole turntable is embedded in the center of the quantitative disc, the turntable is coaxial with the quantitative disc, the quantitative disc substrate corresponds to the turntable substrate in position and height, the quantitative disc cover plate corresponds to the turntable cover plate in position and height, the whole turntable can rotate relative to the quantitative disc, and the gating channel switches the circulation channel on the quantitative disc between a sample introduction storage state or a quantitative sampling state through the rotation of the turntable; the rotary disk and the quantitative disk are hermetically connected, the circular ring at the quantitative disk I in FIG. 4 is a concave part and is used as a bracket of the rotary disk, and the quantitative disk and the rotary disk are both made of organic polymer material, preferably polymethyl methacrylate (PMMA) material, which has the characteristics of toughness, hardness, easy machining, especially excellent biocompatibility, no pollution to biological cells, and transparent material and easy observation.

The turntable and the quantitative disc adopt a hot-pressing bonding method and form a flow passage and a gating passage for liquid flow: covering a quantitative disc cover plate on a quantitative disc substrate, covering the rotary disc cover plate on the rotary disc substrate, moving the rotary disc substrate into a heating chip, raising the temperature, vitrifying an organic polymer, applying pressure between the quantitative disc cover plate and the heating chip, then cooling, removing the pressure and demoulding to seal the quantitative disc cover plate with the quantitative disc substrate, sealing the rotary disc cover plate with the rotary disc substrate, communicating the quantitative disc with the rotary disc at the communication position by using a labyrinth seal structure 15 shown in figure 7, simultaneously ensuring the integral sealing performance of the chip, wherein the labyrinth seal structure 15 is a plurality of annular seal teeth which are sequentially arranged around the quantitative disc and the rotary disc respectively, a series of closure gaps and expansion cavities are formed between the teeth, and a throttling effect is generated when a sample liquid medium passes through the gaps of a zigzag labyrinth to achieve the purpose of leakage resistance; because there is the clearance between labyrinth seal structure's carousel and the quantitative dish, there is not the solid contact, need not lubricate to there is thermal expansion to allow, can adapt to the occasion of high temperature, high pressure, high rotational speed frequency, avoided when six-way valve injector handle rotation is in between sample position and advance a kind position, because rotate and produce too high pressure and cause the problem that the column cap damaged.

Referring to fig. 3 and 4, three flow channels are formed on the quantitative disc substrate, including a liquid storage channel 5, a waste liquid channel 6, and a quantitative channel 7, the liquid storage channel 5, the waste liquid channel 6, and the quantitative channel 7 are all spirally formed on the quantitative disc substrate, the liquid storage channel 5 is used for temporarily storing sample liquid to be quantitatively extracted, so that the volume of the liquid storage channel 5 is greater than that of the quantitative channel 7, one end of the liquid storage channel 5 is selectively communicated with the turntable, the other end of the liquid storage channel 5 is provided with a liquid inlet 1, and the liquid inlet 1 extends to a cover plate of the quantitative disc and is communicated with an external liquid storage device;

the quantitative channel 7 is used for quantitatively measuring sample liquid to be measured, the two ends of the quantitative channel 7 are a sample introduction end and a sampling end, the quantitative channel 7 is communicated with the rotary table through the sample introduction end and the sampling end, and the quantitative channel on the quantitative disc is enabled to be between a sample introduction storage state and a quantitative sampling state by means of rotation of the rotary table; the quantitative disc cover plate is provided with a first interface 3 and a second interface 4, the first interface 3 is used for being connected with an external driving pump, the second interface 4 is used for being connected with an external sampling pump, the sample introduction end of the quantitative channel 7 can be communicated with the external driving pump through the first interface 3, the other end of the quantitative channel 7 is a sampling end, the sampling end of the quantitative channel 7 can be communicated with the external sampling pump through the second interface 4, during sample introduction, the driving pump and the sampling pump provide positive pressure at the first interface 3 and the second interface 4 to enable sample liquid to be detected to flow along a circulation channel, during sampling, the driving pump provides positive pressure for the sample liquid to be detected in the quantitative channel 7, and the sampling pump provides negative pressure for the sample liquid to be detected to enable the sample liquid to be detected to be sampled for standby; the volume of the quantitative channel 7 is 3 μ L to 150 μ L, and may be further preferably 30 μ L, 50 μ L, 100 μ L or 150 μ L, and in this embodiment is preferably 150 μ L, and the volume of the quantitative channel 7 is the volume of the liquid to be measured, in practice, the minimum volume of the quantitative channel 7 may be set according to the required sample injection amount, and when the number of sample liquids required by the microfluidic chip is large, multiple sample injections may be performed through the quantitative channel 7;

6 one end of waste liquid passageway and carousel intercommunication, the 6 other ends of waste liquid passageway set up overflow mouth 2, waste liquid passageway 6 is used for showing in the chip whether quantitative passageway 7 has been full of by liquid, if there is liquid to get into in the waste liquid passageway 6, then explain stock solution passageway 5, quantitative passageway 7 and waste liquid passageway 6 link up in proper order, and be full of by the liquid that awaits measuring in the quantitative passageway 7, can rotate the carousel switching gating passageway and take a sample, setting up of waste liquid passageway 6 is to guaranteeing that sample liquid does not spill over, avoid polluting the chip, surplus liquid in the waste liquid passageway 6 that overflow mouth 2 can be convenient is removed.

As shown in fig. 5 and 6, three gating channels are disposed on the turntable, including a first gating channel 8, a second gating channel 9, and a third gating channel 10, the first gating channel 8, the second gating channel 9, and the third gating channel 10 are three arc channels with equal volumes and same shapes, and the first gating channel 8, the second gating channel 9, and the third gating channel 10 are uniformly distributed on the same circumferential surface of the turntable substrate; the rotary table rotates to different positions relative to the quantitative disc through the gating channel, so that the chip is gated in a sampling storage state or a quantitative sampling state, the sampling storage state is that the liquid storage channel 5, the gating channel I8, the quantitative channel 7 and the waste liquid channel 6 are sequentially communicated, when in a liquid inlet state, the driving pump and the sampling pump provide positive pressure at the interface I3 and the interface II 4, so that sample liquid to be detected flows along the circulation channel, the sample liquid to be detected does not overflow from the interface I3 and the interface II 4, the sample liquid to be detected enters the chip from the liquid inlet 1, and sequentially passes through the liquid storage channel 5, the gating channel I8, the quantitative channel 7 and the waste liquid channel 6, when the sample liquid overflows in the waste liquid channel 6, the liquid storage channel 5, the quantitative channel 7 and the waste liquid channel 6 are sequentially communicated, and the quantitative channel 7 is filled with the quantitative sample liquid to be detected; the quantitative sampling state is that the gating channel III 10, the quantitative channel 7 and the gating channel II 9 are communicated in sequence, the driving pump provides positive pressure for the quantitative channel 7 at the interface I3 at the moment, the sampling pump provides negative pressure at the interface II 4, driving sample liquid enters the sampling pump from the interface II 4, the liquid storage channel 5 is communicated with the waste liquid channel 6 at the moment, and the channel is vacant.

Referring to fig. 3 and 4, a position limiting component is disposed between the rotary table and the quantitative disc, the position limiting component includes a spring 11, a positioning steel ball 12, a sample positioning slot 13 and a sampling positioning slot 14, a positioning hole is disposed at a position on the substrate of the quantitative disc contacting with the rotary table, the spring 11 and the positioning steel ball 12 are disposed in the positioning hole, the spring 11 is connected with the bottom of the positioning hole, the positioning steel ball 12 is disposed at the top of the spring 11, the sample positioning slot 13 and the sampling positioning slot 14 are hemispherical, the diameters of the sample positioning slot 13 and the sampling positioning slot 14 are smaller than the diameter of the positioning steel ball 12, the distance between the sample positioning slot 13 and the sampling positioning slot 14 is 60 ° on the arc surface of the rotary table, the positioning steel ball 12 can be clamped in the sample positioning slot 13 or the sampling positioning steel ball 12 is clamped in the sample positioning slot 13, the circulation channel is in a quantitative sampling storage state, or the rotary table is rotated counterclockwise by 60 °, the positioning steel ball 12 is clamped in the sampling positioning slot 14, the circulation channel is in a quantitative sampling state, the position limiting component is not only used for limiting relative sliding between the rotary table and the rotary angle of the rotary table and the quantitative disc.

Further optimize, add supplementary advance a kind constant head tank 131 and supplementary sample constant head tank 141, the border of carousel apron sets up supplementary advance a kind constant head tank 131 and supplementary sample constant head tank 141, advance a kind constant head tank 13 and sample constant head tank 14 and set up at carousel substrate border, supplementary advance a kind constant head tank 131 and advance a kind constant head tank 13 size equal, the position is corresponding, supplementary sample constant head tank 141 and sample constant head tank 14 size equal, the position is corresponding, advance a kind constant head tank 13 and supplementary advance a kind constant head tank 131 and constitute whole hemispherical groove, supplementary sample constant head tank 141 and sample constant head tank 14 constitute whole hemispherical groove, the setting of supplementary advance a kind constant head tank 131 and supplementary sample constant head tank 141 is convenient for carousel apron and carousel substrate position's affirmation, be convenient for fix a position when linking carousel substrate and carousel apron.

The utility model discloses the vulnerable part of chip is spacing chip's spring and location steel ball 12, because the life-span of spring is limited, can lead to fixing a position 12 screens of steel ball inaccurate, and then leads to the ration sample liquid data misalignment, but the convenience is changed to spring and location steel ball 12, and the cost is also lower.

When the sampling device is used, the turntable is rotated, the positioning steel balls 12 are clamped in a hemispherical groove formed by the sample introduction positioning groove 13 and the auxiliary sample introduction positioning groove 131, the liquid storage channel 5 is communicated with one end of the first gating channel 8, the other end of the first gating channel 8 is communicated with the sample introduction end of the quantitative channel 7, the sampling end of the quantitative channel 7 is communicated with the second gating channel 9, and the third gating channel 10 is vacant, so that the chip is in a sample introduction storage state, please refer to the graph 1, a worker pumps enough liquid to be detected into the liquid storage channel 5 from the liquid inlet 1, redundant reagents can flow into the waste liquid channel 6, when liquid enters the waste liquid channel 6, the liquid storage channel 5 and the quantitative channel 7 are sequentially communicated, the quantitative channel 7 is filled with the liquid to be detected, and the sample introduction is stopped at the moment; afterwards, the turntable is rotated anticlockwise by 60 degrees and switched to a quantitative sampling state for sampling, the positioning steel balls 12 are clamped in a hemispherical groove formed by the sampling positioning groove 14 and the auxiliary sampling positioning groove 141, so that the sample introduction end of the quantitative channel 7 is communicated with the gating channel three 10, the sampling end of the quantitative channel 7 is communicated with the gating channel two 9, the liquid storage channel 5 is communicated with the waste liquid channel 6 through the gating channel one 8, namely, the gating channel one 8, the gating channel two 9 and the gating channel three 10 are in an idle state, at the moment, the chip is in the quantitative sampling state, please refer to fig. 2, at the moment, a worker utilizes the driving pump to provide positive pressure for the quantitative channel 7 at the interface one 3, utilizes the driving pump to provide negative pressure at the interface two 4, and drives liquid in the quantitative channel 7 to enter the sampling pump from the interface two 4, and sampling is completed.

The utility model discloses the chip is based on the principle of six-way valve, when being in the storage state of advancing a kind, the stock solution is through advancing a kind needle from inlet 1 injection stock solution passageway 5, and the stock solution passes through stock solution passageway 5, gating passageway 8, ration passageway 7 and waste liquid passageway 6 in proper order, has the stock solution to get into in waste liquid passageway 6, explains that stock solution passageway 5 and ration passageway 7 link up in proper order, and is full of by the liquid that awaits measuring in the ration passageway 7, has filled up quantitative sample liquid that awaits measuring in the ration passageway 7 this moment; when the rotation switches to the sampling position, the ration sample state is gating passageway three 10, ration passageway 7 and gating passageway two 9 communicate in proper order, utilize the driving pump to provide the malleation in interface 3 department for ration passageway 7, utilize the driving pump to provide the negative pressure in interface two 4 departments, liquid in the drive ration passageway 7 gets into the sampling pump from interface two 4 and carries out the analysis, accomplishes the sample, but compares in current six-way valve, the utility model discloses the chip has not only realized the high accuracy ration sample of the appearance liquid that awaits measuring, and its structure is simpler, and whole body size is less, and is easily integrated, has avoided changing inconvenient and the higher problem of cost after the device wearing parts damages simultaneously, has effectively prolonged the life of chip.

The drive pump and the sampling pump are both preferably FLOW-EZ pressure pumps, the micro-fluidic sampling device is suitable for high-precision high-stability micro-fluidic sampling, the FLOW-EZ has extremely low response time and FLOW control stability, and compared with a high-precision injection pump, the FLOW-EZ has very obvious advantages in FLOW control and does not have impulse response; meanwhile, the FLOW-EZ integrated knob and the display screen are convenient to use, the output pressure can be adjusted by rotating the knob, positive pressure and negative pressure can be provided for the microfluidic chip, parameters such as a pressure set value, a pressure output value, FLOW and the like can be displayed on the display screen in real time, the FLOW monitoring module can be matched to realize control of fluid, and the operation is simple and rapid.

The second embodiment:

referring to fig. 8 and 9, the present invention provides a second simplified microfluidic chip for quantitative sampling: the quantitative disc substrate is provided with three circulation channels, each circulation channel comprises a liquid storage channel 5, a quantitative channel 7 and a waste liquid channel 6, one end of each quantitative channel 7 is provided with a sample introduction end, the other end of each quantitative channel 7 is provided with a sampling end, one end of each liquid storage channel 5 is provided with a liquid inlet 1 communicated with liquid storage equipment, the other end of each liquid storage channel 5 is selectively communicated with the sample introduction end of each quantitative channel 7 through a gating channel, one end of each waste liquid channel 6 is provided with a liquid overflow port 2 communicated with the outside, and the other end of each waste liquid channel 6 is selectively communicated with the sampling end of each quantitative channel 7 through a gating channel; set up interface one 3 and interface two 4 on the quantitative dish apron, interface one 3 is used for being connected with outside drive pump, and interface two 4 is used for being connected with outside sampling pump, and quantitative passageway 7's introduction of the sample end can be through interface one 3 and outside drive pump intercommunication, and quantitative passageway 7's the other end is the sample end, and quantitative passageway 7's sample end can be through interface two 4 and outside sampling pump intercommunication.

The turntable is provided with two gating channels, namely a gating channel I8 and a gating channel II 9, wherein the gating channel I8 is communicated with the interface I3, and the gating channel II 9 is communicated with the interface II 4; when one end of the liquid storage channel 5 is communicated with external liquid storage equipment, the other end of the liquid storage channel 5 is communicated with a sample introduction end of the quantitative channel 7 through the first gating channel 8, and a sampling end of the quantitative channel 7 is communicated with one end of the waste liquid channel 6 through the second gating channel 9, a sample introduction storage state is achieved at the moment, if liquid enters the waste liquid channel 6, the liquid storage channel 5, the quantitative channel 7 and the waste liquid channel 6 are sequentially communicated, and the quantitative channel 7 is filled with liquid to be detected; the sample injection can be stopped, then the turntable is rotated clockwise for 180 degrees to be switched to a quantitative sampling state, the sample injection end of the quantitative channel 7 is communicated with the gating channel I9, the sample injection end of the quantitative channel 7 is communicated with the gating channel I8, the interface II 4 is communicated with the sampling pump, the liquid storage channel 5 and the waste liquid channel 6 are not communicated in a vacant mode, and the quantitative sampling state is achieved.

When the sampling device is used, the outlet of the gating channel I8 is communicated with the sampling end of the quantitative channel 7, a sample to be detected enters the gating channel through the liquid inlet 1 of the liquid storage channel 5 and enters the sampling end of the quantitative channel 7 from the port communication part of the gating channel I8, the sampling end of the quantitative channel 7 is communicated with the waste liquid channel 6 through the gating channel II 9, and the chip of the utility model is in a sampling storage state; when the quantitative channel 7 is full of liquid to be measured, the excessive liquid in the chip overflows from the overflow port 2 of the waste liquid channel 6 at the moment, the sample introduction is required to be stopped, the rotary table is rotated 180 degrees, so that the gating channel II 9 is communicated with the sample introduction end of the quantitative channel 7, the gating channel I8 is communicated with the sampling end of the quantitative channel 7, the chip is in a quantitative sampling state, the driving pump provides positive pressure for the quantitative channel 7 at the interface I3, the sampling pump provides negative pressure at the interface II 4, and the driving sample liquid enters the sampling pump from the interface II 4 and is output for use.

Example three: referring to the quantitative sampling state structure of the four-gate channel chip shown in fig. 10, the difference between the first embodiment and the second embodiment is: the volume of the quantitative channel 7 is preferably 100 mu L, four gating channels are arranged on the substrate of the turntable, the four gating channels are arc-shaped channels with equal volumes and same shapes, the four gating channels are uniformly distributed on the substrate of the turntable, the four gating channels are opposite in pairs, two opposite gating channels form a group, one group of channels are used for sampling storage state, the other group of channels are used for quantitative sampling state, and when switching is carried out, the turntable needs to rotate 90 degrees, and the sampling storage state is switched to the quantitative sampling state.

Example four: referring to the structure of the five-gate channel chip with the quantitative sampling status as shown in fig. 11, the present embodiment is different from the first embodiment in that: the volume of the quantitative channel 7 is preferably 50 μ L, five gating channels are arranged on the substrate of the turntable, the five gating channels are arc-shaped channels with equal volumes and same shapes, the five gating channels are uniformly distributed on the substrate of the turntable, two opposite gating channels are selected as one group, one group of channels is used for a sample storage state, and one group of channels is used for a quantitative sampling state, as shown in fig. 9, when switching is carried out, the turntable is required to rotate 72 degrees, and the sample storage state is switched to the quantitative sampling state.

Example five: referring to the quantitative sampling state structure of the six-gate channel chip shown in fig. 12, the difference between the first embodiment and the second embodiment is: the volume of the quantitative channel 7 is preferably 30 mu L, six gating channels are arranged on a turntable substrate, the six gating channels are arc-shaped channels with equal volumes and same shapes, the six gating channels are uniformly distributed on the turntable substrate, the six gating channels are opposite in pairs, any two opposite gating channels form a group, namely, three groups of channels are provided, and the three groups of channels can be used as a sample storage state or a quantitative sampling state.

The utility model discloses also can set up a plurality of gating passageways in the chip is actual, the carousel rotation angle's of being convenient for design and the overall arrangement of each passageway make things convenient for each gating passageway and the external equipment intercommunication of chip to the quantitative sampling state of the chip of being convenient for and the switching of advancing kind storage state.

Claims (9)

1. A microfluidic chip for quantitative sampling, characterized by: the quantitative disc comprises a rotary disc and a quantitative disc, wherein the rotary disc is embedded in the quantitative disc and rotates relative to the quantitative disc;

a circulating channel for sample introduction storage and quantitative sampling of the sample liquid to be detected is arranged on the quantitative disc;

the rotary disc is provided with a gating channel, and the gating channel realizes the switching of the circulation channel on the quantitative disc between a sample storage state and a quantitative sampling state through the rotation of the rotary disc.

2. The microfluidic chip for quantitative sampling according to claim 1, wherein: the circulation channel comprises a quantitative channel (7), one end of the quantitative channel (7) is a sample introduction end, the sample introduction end of the quantitative channel (7) is provided with a first interface (3) communicated with the outside, and the first interface (3) is communicated with the driving pump; the other end of the quantitative passage (7) is a sampling end, the sampling end of the quantitative passage (7) is provided with a second interface (4) communicated with the outside, and the second interface (4) is communicated with a sampling pump;

the circulation channel further comprises a liquid storage channel (5), one end of the liquid storage channel (5) is provided with a liquid inlet (1) communicated with external liquid storage equipment, and the other end of the liquid storage channel (5) is selectively communicated with a sample introduction end of the quantitative channel (7) through a gating channel;

during sample injection, the liquid storage channel (5) is communicated with the sample injection end of the quantitative channel (7) through a gating channel, the sampling end of the quantitative channel (7) is vacant and is not communicated, and the circulation channel is in a sample injection storage state at the moment; or, the rotating disc is rotated, the liquid storage channel (5), the quantitative channel (7) and the gating channel are all vacant and are not communicated, and the flow channel is in a quantitative sampling state at the moment.

3. The microfluidic chip for quantitative sampling according to claim 2, wherein: the circulation channel also comprises a waste liquid channel (6), one end of the waste liquid channel (6) is provided with a spillway port (2) communicated with the outside, and the other end of the waste liquid channel (6) is selectively communicated with a sampling end of the quantitative channel (7) through a gating channel;

the two gating channels are respectively a gating channel I (8) and a gating channel II (9), the gating channel I (8) and the gating channel II (9) are two arc-shaped channels with equal volumes, and the gating channel I (8) and the gating channel II (9) are oppositely arranged on the turntable;

the liquid storage channel (5) is communicated with the sample introduction end of the quantitative channel (7) through a gating channel I (8), the sampling end of the quantitative channel (7) is communicated with the waste liquid channel (6) through a gating channel II (9), and the circulation channel is in a sample introduction storage state at the moment; or, the turntable is rotated, the second gating channel (9) is communicated with the sampling end of the quantitative channel (7), the sampling end of the quantitative channel (7) is communicated with the first gating channel (8), the liquid storage channel (5) and the waste liquid channel (6) are both idle and not communicated, and the circulation channel is in a quantitative sampling state at the moment.

4. The microfluidic chip for quantitative sampling according to claim 2, wherein: the circulation channel also comprises a waste liquid channel (6), one end of the waste liquid channel (6) is provided with a spillway port (2) communicated with the outside, and the other end of the waste liquid channel (6) is selectively communicated with a sampling end of the quantitative channel (7) through a gating channel;

the gating channels are three and respectively comprise a first gating channel (8), a second gating channel (9) and a third gating channel (10), the first gating channel (8), the second gating channel (9) and the third gating channel (10) are three arc-shaped channels with equal volumes, and the first gating channel (8), the second gating channel (9) and the third gating channel (10) are uniformly distributed on the turntable;

the liquid storage channel (5) is communicated with a sample introduction end of the quantitative channel (7) through a gating channel I (8), a sampling end of the quantitative channel (7) is communicated with the waste liquid channel (6) through a gating channel II (9), a gating channel III (10) is vacant and is not communicated at the moment, and a circulation channel is in a sample introduction and storage state at the moment; or the turntable is rotated, the gating channel III (10) is communicated with the sample introduction end of the quantitative channel (7), the sampling end of the quantitative channel (7) is communicated with the gating channel II (9), the liquid storage channel (5), the waste liquid channel (6) and the gating channel I (8) are all empty and are not communicated, and the circulation channel is in a quantitative sampling state.

5. The microfluidic chip for quantitative sampling according to claim 3 or 4, wherein: the quantitative disc comprises a quantitative disc substrate and a quantitative disc cover plate, the quantitative disc cover plate is bonded on the quantitative disc substrate, and the flow channel is a cavity between a liquid flow groove body arranged on the quantitative disc substrate and the quantitative disc cover plate;

the rotary table comprises a rotary table substrate and a rotary table cover plate, the rotary table cover plate is bonded on the rotary table substrate, and the gating channel is a cavity between a liquid flow groove body arranged on the quantitative disc substrate and the quantitative disc;

the turntable is integrally embedded in the quantitative disc, the quantitative disc substrate corresponds to the turntable substrate in position and height, and the quantitative disc cover plate corresponds to the turntable cover plate in position and height; the circulation channel and the gating channel realize the switching of the circulation channel between a sample storage state and a quantitative sampling state through the rotation of the turntable;

the liquid inlet (1) and the overflow port (2) are arranged on the surface of the cover plate of the quantitative disc, the liquid storage channel (5) is communicated with the outside through the liquid inlet (1), and the waste liquid channel (6) is communicated with the outside through the overflow port (2); the first interface (3) and the second interface (4) are arranged on the surface of the quantitative disc cover plate, the sampling end of the quantitative channel (7) is communicated with the outside through the first interface (3), and the quantitative channel (7) is communicated with the sampling pump through the second interface (4).

6. The microfluidic chip for quantitative sampling according to claim 5, wherein: the volume of the quantitative channel (7) is 3-150 mu L, and the volume of the liquid storage channel (5) is larger than that of the quantitative channel (7).

7. The microfluidic chip for quantitative sampling according to claim 6, wherein: the port communication part of the flow passage on the quantitative disc and the port communication part of the gating passage on the rotary disc are both labyrinth seal structures (15).

8. The microfluidic chip for quantitative sampling according to claim 7, wherein: a limiting assembly is arranged between the rotary disc and the quantitative disc, the limiting assembly comprises a spring (11), a positioning steel ball (12), a sample feeding positioning groove (13) and a sampling positioning groove (14), the quantitative disc is provided with a positioning hole, the spring (11) and the positioning steel ball (12) are arranged in the positioning hole, the spring (11) is connected with the bottom of the positioning hole, and the positioning steel ball (12) is arranged at the top of the spring (11); advance kind constant head tank (13) and sample constant head tank (14) and set up on the carousel, advance kind constant head tank (13) and sample constant head tank (14) and all be the hemisphere, location steel ball (12) block is advancing kind constant head tank (13) or sample constant head tank (14), location steel ball (12) block is advancing kind constant head tank (13) in, and the circulation passageway is in and advances kind memory state, perhaps, rotatory carousel, location steel ball (12) block is in sample constant head tank (14), and the circulation passageway is in the quantitative sample state.

9. The microfluidic chip for quantitative sampling according to claim 8, wherein: the border of carousel apron sets up supplementary appearance constant head tank (131) and supplementary sample constant head tank (141), it sets up on the carousel substrate to advance appearance constant head tank (13) and sample constant head tank (14), supplementary appearance constant head tank (131) equals with a kind constant head tank (13) size, and the position is corresponding, supplementary sample constant head tank (141) equals with sample constant head tank (14) size, and the position is corresponding, it wholly is hemispherical groove to advance appearance constant head tank (13) and supplementary appearance constant head tank (131) constitution, supplementary sample constant head tank (141) constitutes whole hemispherical groove with sample constant head tank (14).

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