CN218786620U

CN218786620U - Cell culture device

Info

Publication number: CN218786620U
Application number: CN202221385401.XU
Authority: CN
Inventors: 何修业; 卢杰
Original assignee: Guangzhou Xinyuan Biotechnology Co ltd
Current assignee: Guangzhou Xinyuan Biotechnology Co ltd
Priority date: 2022-06-02
Filing date: 2022-06-02
Publication date: 2023-04-04
Anticipated expiration: 2032-06-02

Abstract

The utility model belongs to the technical field of cell culture, in particular to a cell culture device, which comprises an incubator and a controller, wherein the controller is arranged at the right end of the incubator, the upper end of the incubator is provided with a closed cover, the center of the inside of the incubator is fixedly provided with a mounting plate, the middle part of the upper end of the mounting plate is provided with a through groove, the inside of the through groove is sleeved with a placing table, the upper side of one side of the circumference of the placing table is provided with a plurality of engaging grooves in a surrounding way, and one side of the circumference of a round block is provided with engaging teeth which are engaged with the engaging grooves; a motor is fixedly arranged on the lower side of the inner wall of the left side of the incubator, and a rotating shaft is rotatably assembled on the lower side of the inner wall of the right side of the incubator; the utility model has the advantages of simple structure and reasonable design, only need starter motor alright when using in order to drive the culture household utensils that are located incubator inside and carry out even and slight shake and rotation from top to bottom, so not only can slow down the liquid of cultivation and congeal, can also make culture solution and cell carry out more intensive mixing, can effectively improve the activity of cell.

Description

Cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, concretely relates to cell culture device.

Background

Cell culture is also called as cell cloning technology, the formal term in biology is called as bioengineering technology, and for some biological cloning technologies, cell culture is an indispensable link, cell culture can differentiate a cell into a plurality of cells, which is an indispensable link in cell culture technology, cell culture generally places cells in a culture vessel, then places the culture vessel in an incubator for cell culture, culture solution is required to be mixed with the cultured cells when culturing the cells, the existing cell culture device usually places the culture vessel in a culture device when in use, and the culture solution in the culture vessel is easy to solidify after long-time standing of the culture vessel, so that the cells are easy to lose viability.

SUMMERY OF THE UTILITY MODEL

The utility model aims at: aim at providing a cell culture device, when using, only need starter motor alright with the culture ware that drives to be located the incubator inside carry out even and slight shake and rotation from top to bottom to shake from top to bottom and rotate for crisscross setting, and be intermittent motion, so not only can slow down the liquid of cultivation and solidify, can also make culture solution and cell carry out more intensive mixing, can effectively improve the activity of cell.

In order to realize the technical purpose, the utility model adopts the following technical scheme:

a cell culture device comprises an incubator and a controller, wherein the controller is installed at the right end of the incubator, a support assembly is installed at the lower end of the incubator, an air evolution mechanism is installed at the right end of the incubator, a sealing cover is arranged at the upper end of the incubator, a mounting plate is fixedly installed in the center of the inside of the incubator, a through groove is formed in the middle of the upper end of the mounting plate, a placing table is sleeved inside the through groove, a plurality of placing grooves are formed in the upper end of the placing table in a surrounding mode, a plurality of meshing grooves are formed in the upper side of one side of the circumference of the placing table in a surrounding mode, a round block is rotatably installed on the right side of the upper end of the mounting plate, and meshing teeth meshed with the plurality of meshing grooves are arranged on one side of the circumference of the round block;

the utility model discloses a temperature control device for incubator, including incubator left side inner wall, pivot, mounting panel, spring lower extreme fixed mounting, pivot, cam, transmission assembly, incubator internally mounted have a temperature control subassembly, motor and controller electric connection, the downside fixed mounting of incubator left side inner wall has the motor, the downside of incubator right side inner wall is rotated and is equipped with the pivot, the output shaft and the pivot transmission of motor are connected, the mounting panel lower extreme is encircleed and is installed a plurality of springs, and is a plurality of spring lower extreme fixed mounting has the roof, pivot circumference one side middle part fixed mounting has the cam, roof lower extreme fixed mounting have with cam assorted butt pole, butt pole lower extreme and cam circumference one side looks butt, roof upper end fixed mounting has the connecting rod, the connecting rod upper end with place the platform and rotate and be connected, install transmission assembly between pivot and the round piece, incubator internally mounted has the temperature control subassembly, motor and controller electric connection.

The transmission assembly comprises a first bevel gear, the lower end of the round block is fixedly mounted on the rotating rod, the lower end of the rotating rod extends out of the mounting plate, the lower end of the rotating rod is fixedly mounted on the first bevel gear, and a second bevel gear meshed with the first bevel gear is fixedly mounted on one side of the circumference of the rotating shaft.

And a plurality of stabilizing sleeves are fixedly arranged in the placing grooves.

The temperature control assembly comprises a temperature controller, the temperature controller is fixedly installed on the upper side inside the incubator, a refrigerator and a heater are installed at the rear end of the incubator, the output ends of the refrigerator and the heater extend into the incubator, and the temperature controller, the refrigerator and the heater are electrically connected with the controller.

The supporting component comprises four supporting columns, and the four supporting columns are uniformly distributed and installed at the lower end of the incubator.

A ring groove is formed in one side of the circumference of the cam, a roller is installed at the lower end of the abutting rod, and the roller is abutted to the bottom side inside the ring groove.

The utility model discloses when using, only need starter motor alright in order to drive the culture household utensils that are located incubator inside and carry out even and slight upper and lower shake and rotation to shake from top to bottom and rotate for crisscross setting, and be intermittent motion, the liquid that not only can slow down the cultivation like this solidifies, can also make culture solution and cell carry out more intensive mixing, can effectively improve the activity of cell, and can be through the living environment of air evolution mechanism and temperature control subassembly to reinforcing cell culture, increase the cultivation efficiency of cell.

Drawings

The invention can be further illustrated by means of non-limiting examples given in the figures.

FIG. 1 is a first schematic structural diagram of a first embodiment of a cell culture apparatus according to the present invention;

FIG. 2 is a schematic view of a first embodiment of a cell culture apparatus according to the present invention;

FIG. 3 is a third schematic structural view of a first embodiment of the cell culture apparatus of the present invention;

FIG. 4 is a schematic view of a first embodiment of a cell culture apparatus according to the present invention;

FIG. 5 is a schematic diagram of a second partial sectional structure of a first cell culture apparatus according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a second embodiment of the cell culture apparatus of the present invention;

FIG. 7 is a second schematic view of a cell culture apparatus according to a second embodiment of the present invention;

FIG. 8 is a schematic sectional view of a cell culture apparatus according to a second embodiment of the present invention;

FIG. 9 is an enlarged view of the structure at A in FIG. 8;

the main element symbols are as follows:

the first embodiment is as follows: the incubator comprises an incubator 1, a controller 11, a closing cover 12, a mounting plate 13, a through groove 14, a placing table 15, a placing groove 16, an engaging groove 17, a round block 18, an engaging tooth 19, a motor 20, a rotating shaft 21, a spring 22, a top plate 23, a cam 24, an abutting rod 25 and a connecting rod 26;

example two: the device comprises a first bevel gear 27, a rotating rod 28, a second bevel gear 29, a stabilizing sleeve 30, an ultraviolet germicidal lamp 31, a vent 32, a sleeve 33, a filter plate 34, a temperature controller 35, a refrigerator 36, a heater 37, a supporting column 38, a ring groove 39 and a roller 40.

Detailed Description

In order to make the present invention better understood by those skilled in the art, the technical solutions of the present invention are further described below with reference to the accompanying drawings and examples.

The first embodiment is as follows:

as shown in fig. 1-5, the utility model discloses a cell culture device, including incubator 1 and controller 11, controller 11 is installed in incubator 1 right-hand member, the supporting component is installed to incubator 1 lower extreme, incubator 1 right-hand member is installed the air evolution mechanism, incubator 1 upper end is equipped with closing cap 12, incubator 1 inside central fixed mounting has mounting panel 13, mounting panel 1 upper end middle part has seted up logical groove 14, the cover is equipped with placing table 15 inside logical groove 14, placing table 15 upper end has been encircleed and has been seted up a plurality of standing grooves 16, placing table 15 circumference one side upside has been encircleed and has been seted up a plurality of meshing grooves 17, mounting panel 13 upper end right side is rotated and is equipped with circle piece 18, circle piece 18 circumference one side is equipped with meshing tooth 19 with a plurality of meshing grooves 17 engaged with;

downside fixed mounting of 1 left side inner wall of incubator has motor 20, the downside of 1 right side inner wall of incubator rotates and is equipped with pivot 21, motor 20's output shaft is connected with the transmission of pivot 21, mounting panel 13 lower extreme encircles and installs a plurality of springs 22, a plurality of springs 22 lower extreme fixed mounting have roof 23, pivot 21 circumference one side middle part fixed mounting has cam 24, roof 23 lower extreme fixed mounting have with cam 24 assorted butt rod 25, butt rod 25 lower extreme and cam 24 circumference one side looks butt, roof 23 upper end fixed mounting has connecting rod 26, connecting rod 26 upper end is rotated with placing platform 15 and is connected, install transmission assembly between pivot 21 and the circle piece 18, 1 internally mounted of incubator has temperature control assembly, motor 20 and 11 electric connection of controller.

When the cells need to be cultured, the working personnel open the sealing cover of the incubator 1, can place the cells and culture solution into culture vessels in advance, then place the culture vessels into the placing table 15, and place a plurality of culture vessels into the placed groove 16, so that the efficiency of cell culture can be increased, the working personnel can close the sealing cover 12, and can control the temperature inside the incubator 1 through a temperature control assembly, so that the temperature inside the incubator 1 can not be too high or too low, and the cells can have a better living environment when being cultured, and an air purification mechanism can purify the outside air when the cells are cultured, so that the inside of the incubator 1 can not be polluted by outside impurities when being ventilated, and the quality of the cell culture is improved;

during the cell culture process, the worker starts the operation of the motor 20 through the control end of the controller 11, when the motor 20 is operated, the output shaft of the motor 20 rotates slowly to drive the rotating shaft 21 to rotate slowly, when the rotating shaft 21 rotates, the rotating shaft 21 drives the cam 24 to rotate, because the lower end of the abutting rod 25 is constantly kept in an abutting state with one side of the circumference of the cam 24, so that when the cam 24 rotates, the cam 24 is mainly divided into two parts, namely a smooth part and a convex part, the smooth part is in a regular circular shape, so that when the abutting rod 25 abuts with the smooth part of the cam 24, even if the cam 24 rotates, the diameter of the cam 24 does not change when the cam rotates, the cam 24 does not push the abutting rod 25 upwards, when the cam 24 rotates to the convex part, the lower end of the abutting rod 25 contacts with the convex part of the cam 24, which corresponds to a circle with the diameter increasing diameter, the abutting rod 25 pushes the abutting rod 25 upwards, because the spring 22 is arranged, in this state, the abutting rod 25 constantly abuts with the cam 24, so that the convex part of the cam 24 is constantly pressed upwards, the top plate 23, and the top plate 15 is constantly pressed, so that the culture solution is constantly placed on the top plate 15, and the top plate 15 is constantly pressed, so that the culture dish is placed on the top plate 15, and the top plate 15 is constantly moves, so that the culture dish is placed on the top plate 15, and the top plate 15 is placed in a reciprocating table 15, and the dish is constantly moves, so that the dish is placed on the dish is placed in a reciprocating table 15, and the dish is constantly moves, and the dish is placed in a state, and the dish is constantly pressed, so that the dish is placed on the dish is placed in a state, and the dish 15, the dish is placed in a state, so that the dish 15, the dish is constantly pressed by the top plate 15, the dish is placed in a centrifugal force of the dish, and the dish is constantly, the culture solution and the cells are fully mixed, and the activity of the cells is increased;

when the placing table 15 moves up and down, the transmission assembly is arranged between the rotating shaft 21 and the round block 18 and can drive the round block 18 to rotate when the rotating shaft 21 rotates, when the round block 18 rotates until the meshing teeth 19 are meshed with the meshing grooves 17, the placing table 15 can be driven to rotate, so that culture solution can be driven to flow due to rotating centrifugal force, the culture solution and cells can be more fully mixed while solidification of the cultured solution is further slowed down, when the meshing teeth 19 are not meshed with the meshing grooves 17, the placing table 15 cannot rotate, the arrangement positions of the meshing teeth 19 and the convex parts of the cams 24 are in staggered arrangement, when the convex parts of the cams 24 upwards push the placing table 15, the meshing teeth 19 cannot be meshed with the meshing grooves 17, when the abutting rods 25 abut against the smooth parts of the cams 24, the round block 18 rotates until the meshing teeth 19 are meshed with the meshing grooves 17, the placing table 15 is driven to rotate, and the staggered movement arrangement is achieved, so that the movement between the two parts is not interfered with each other, and the placing table is simple and convenient to use;

the utility model discloses when using, only need starter motor 20 alright in order to drive the culture household utensils that are located 1 inside of incubator and carry out even and slight shake and rotation from top to bottom to shake and rotate for crisscross setting, and be intermittent motion, the liquid that so not only can slow down the cultivation solidifies, can also make culture solution and cell carry out more intensive mixing, can effectively improve the activity of cell, and can increase the culture efficiency of cell through air evolution mechanism and temperature control subassembly to the living environment of reinforcing cell culture.

The second embodiment:

as shown in fig. 6 to 9, the transmission assembly includes a first bevel gear 27, the lower end of the round block 18 is fixedly mounted on a rotating rod 28, the lower end of the rotating rod 28 extends out of the mounting plate 13, the lower end of the rotating rod 28 is fixedly mounted on the first bevel gear 27, and a second bevel gear 29 engaged with the first bevel gear 27 is fixedly mounted on one side of the circumference of the rotating shaft 21; thus, when the rotating shaft 21 rotates, the rotating shaft 21 can drive the rotating rod 28 to rotate through the engagement of the second bevel gear 29 and the first bevel gear 27, and then the round block 18 is driven to rotate, and the operation is simple and convenient.

Stabilizing sleeves 30 are fixedly arranged in the plurality of placing grooves 16; the stabilizing sleeve 30 can be better matched with a culture vessel, so that the culture vessel is placed more stably.

The temperature control assembly comprises a temperature controller 35, the temperature controller 35 is fixedly arranged on the upper side in the incubator 1, a refrigerator 36 and a heater 37 are arranged at the rear end of the incubator 1, the output ends of the refrigerator 36 and the heater 37 extend into the incubator 1, and the temperature controller 35, the refrigerator 36 and the heater 37 are electrically connected with the controller 11; thus, the temperature controller 11 controls the upper limit and the lower limit of the temperature detected by the temperature controller 35, for example, the temperature is controlled to be between 30 ℃ and 20 ℃, when the temperature is lower than 20 ℃, the temperature controller 30 automatically starts the heater 37 to heat the temperature inside the incubator 1 to be higher than 20 ℃, and when the temperature is higher than 30 ℃, the temperature controller 35 automatically starts the refrigerator 36 to reduce the temperature inside the incubator 1 to be lower than 30 ℃, so that the cells can be effectively cultured in a comfortable temperature environment.

The supporting component comprises four supporting columns 38, and the four supporting columns 38 are uniformly distributed and installed at the lower end of the incubator 1; this provides a stable support for the incubator 1 via the support posts 38.

A circular groove 39 is formed in one side of the circumference of the cam 24, a roller 40 is installed at the lower end of the abutting rod 25, and the roller 40 abuts against the bottom side of the inside of the circular groove 39; this allows the roller 40 to abut the cam 24, reducing friction.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention shall be covered by the claims of the present invention.

Claims

1. The utility model provides a cell culture device, includes incubator and controller, the controller is installed in the incubator right-hand member, its characterized in that: the incubator comprises an incubator body, a supporting assembly, a placing table, a plurality of engaging grooves, a round block and engaging teeth, wherein the incubator body is provided with the supporting assembly at the lower end;

2. A cell culture assembly according to claim 1, wherein: the transmission assembly comprises a first bevel gear, a rotary rod is fixedly mounted at the lower end of the round block, a mounting plate extends out of the lower end of the rotary rod, the first bevel gear is fixedly mounted at the lower end of the rotary rod, and a second bevel gear meshed with the first bevel gear is fixedly mounted on one side of the circumference of the rotary shaft.

3. A cell culture apparatus according to claim 1, wherein: and a plurality of stabilizing sleeves are fixedly arranged in the placing grooves.

4. A cell culture assembly according to claim 1, wherein: the temperature control assembly comprises a temperature controller, the temperature controller is fixedly installed on the upper side inside the incubator, a refrigerator and a heater are installed at the rear end of the incubator, the output ends of the refrigerator and the heater extend into the incubator, and the temperature controller, the refrigerator and the heater are electrically connected with the controller.

5. A cell culture assembly according to claim 1, wherein: the supporting component comprises four supporting columns, and the four supporting columns are uniformly distributed and installed at the lower end of the incubator.

6. A cell culture assembly according to claim 1, wherein: a ring groove is formed in one side of the circumference of the cam, a roller is installed at the lower end of the abutting rod, and the roller is abutted to the bottom side inside the ring groove.