CN218742562U - Blood supernatant preparation facilities - Google Patents

Abstract

The utility model belongs to a blood supernatant preparation device; the blood collecting device comprises a rubber cap used for collecting venous blood and sealing, and a shell matched with the rubber cap to realize that the interior of the shell is in a vacuum environment, wherein a sliding shell used for separating supernatant and extracting supernatant under normal pressure is arranged at the inner upper part of the shell, and an inner lower shell used for realizing blood stratification is sleeved at the lower part of the sliding shell; has the advantages of simple structure, reasonable design, and convenient blood separation and supernatant collection and extraction without changing the container on the premise of direct vein blood collection.

Description

Blood supernatant preparation facilities

Technical Field

The utility model belongs to the technical field of blood products separation draws, specifically are a blood supernatant preparation facilities.

Background

The vacuum blood collection tube is a negative pressure vacuum tube, is matched with a vein blood collection needle for use, and is used for collecting vein blood. The existing vacuum blood collection tube has the characteristics of vacuum, so that the blood collection process is convenient to operate, and the manufacturing cost is low, so that the vacuum blood collection tube is a consumable material which is applicable to the field of blood component separation, but negative pressure can be generated in a container in the aseptic extraction process of blood supernatant components, so that the operation of supernatant extraction is difficult to perform; meanwhile, after the vacuum blood collection tube is used for centrifugal layering, components cannot be physically separated, and the components can be mixed by shaking slightly, so that the blood supernatant is inconvenient to extract.

In order to solve the technical problems, some enterprises adopt that a filter membrane is additionally arranged in a vacuum blood collection tube to separate components such as serum or plasma, but in practical application, the efficiency of filtering blood components by the filter membrane is very slow, the filter membrane can only be used for micro-filtration, and the filter membrane can not bear the centrifugal force during centrifugation of the vacuum blood collection tube. After the conventional vacuum blood collection tube is centrifugally separated, the rubber cap on the upper part of the vacuum blood collection tube is punctured into the needle head, so that the air outside the container can enter the device, the negative pressure is removed, and then the injector is used for extracting the blood supernatant; the rubber cap on the upper part of the vacuum blood collection tube is removed to directly extract blood supernatant, but the methods have higher professional requirements on operators, and the operation failure is easily caused by mixing the supernatant with other components due to shaking, so the risk is higher; and also causes the blood supernatant to be contaminated by contacting with air. Based on this, the vacuum blood collection tube can be used only in a limited range.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome the defect among the prior art, and provide one kind and can be convenient for cooperate the vein blood taking needle to carry out vacuum blood sampling to realize the blood supernatant preparation facilities that separation layering and the ordinary pressure sterile condition of blood extracted the supernatant under the prerequisite of not changing the container behind the whole blood of gathering.

In order to achieve the purpose, the utility model adopts the technical proposal that:

the utility model provides a blood supernatant preparation facilities, this preparation facilities include with be used for gathering venous blood and sealed rubber cap to and cooperate with the rubber cap and realize inside shell for vacuum environment, the interior upper portion of shell is equipped with the slip shell that is used for separating the supernatant and the supernatant is drawed to the ordinary pressure, the lower part cover of slip shell is equipped with the interior inferior valve that is used for realizing blood layering.

Preferably, the outer wall of the upper side of the sliding shell is provided with air holes with antibacterial breathable films; the shell, the sliding shell and the inner lower shell are made of transparent materials.

Preferably, the inside of the top of the sliding shell is connected with the outer wall of the lower part of the rubber cap, the lower part of the sliding shell is provided with a slope-shaped transition surface, the bottom of the slope-shaped transition surface is provided with a separation baffle which is of an integrated structure and extends outwards, the outer side of the joint of the slope-shaped transition surface and the separation baffle is provided with a sealing ring which is matched with the inner wall of the inner lower shell, the inner side of the joint of the slope-shaped transition surface and the separation baffle is matched with the guide sealing supporting part, and the inner side of the joint of the slope-shaped transition surface and the separation baffle is provided with a small groove which is matched with the guide sealing supporting part.

Preferably, the sealed supporting part of direction is including establishing the stand on the inferior valve diapire in, and the outside cover of stand is equipped with the rubber column, and the outer wall of rubber column and the inboard looks adaptation of junction of slope shape transition face and separation baffle.

Preferably, the rubber cap comprises an outer circumferential sealing joint surface part and a central groove arranged at the central position of the top of the rubber cap; the outer circumference sealing joint surface part comprises a lower sealing joint surface arranged at the lower part of the outer circumference, the upper part of the lower sealing joint surface is provided with a lower step limiting surface, the outer side of the upper part of the lower step limiting surface is provided with an upper sealing joint surface, and the upper part of the upper sealing joint surface is provided with an upper step limiting surface.

Preferably, the lower sealing joint surface is matched with the inner wall of the top of the sliding shell, a sliding shell boss is arranged on the outer wall of the upper part of the sliding shell, and the upper surface of the sliding shell boss is matched with the lower step limiting surface; the upper part of the inner wall of the shell is provided with an outward-expanding step, the outer wall of the shell corresponding to the upper part of the step is provided with an external thread, the upper joint surface of the step is matched with the lower surface of the boss of the sliding shell, and the inner wall of the shell at the upper part of the step is matched with the upper sealing joint surface.

Preferably, the rubber cap further comprises a protective cap arranged outside the rubber cap, a protective cap step is arranged on the inner upper portion of the protective cap, an internal thread matched with the external thread on the outer wall of the shell is arranged on the inner wall of the lower portion of the protective cap step, and the upper combining surface of the protective cap step is matched with the limiting surface of the upper step.

Preferably, the diameter of the small groove is 0.1-1 mm.

According to the blood supernatant preparation device manufactured according to the scheme, the purpose of whole blood collection is achieved by arranging the rubber cap and the shell to form a vacuum environment, the supernatant can be separated in a physical mechanical mode after blood layering by arranging the sliding shell and the inner lower shell in the shell, remixing of the supernatant is avoided, and meanwhile, a foundation is laid for normal pressure supernatant extraction; has the advantages of simple structure, reasonable design, and convenient blood separation and supernatant collection and extraction without changing the container on the premise of direct vein blood collection.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the middle and inner lower shells of the present invention.

Fig. 3 is a schematic structural view of the middle rubber column of the present invention.

Fig. 4 is a schematic structural diagram of the middle seal ring of the present invention.

Fig. 5 is a schematic structural view of the sliding shell of the present invention.

Fig. 6 is a partially enlarged view of a portion a in fig. 5.

Fig. 7 is a schematic structural view of the rubber cap of the present invention.

Fig. 8 is a schematic structural diagram of the middle housing of the present invention.

Fig. 9 is a schematic structural view of the middle protective cap of the present invention.

In the figure:

1. an inner lower shell; 2. a rubber column; 3. a seal ring; 4. a sliding shell; 5. a rubber cap; 6. a housing; 7. a protective cap; 8. a bacterium-blocking breathable film; 9. a slope-shaped transition surface; 10. separating the baffle plate; 11. a small groove; 12. a column; 13. a central slot; 14. a boss of the sliding shell; 15. a lower step limiting surface; 16. an upper sealing joint surface; 17. an upper step limiting surface; 18. a helmet step; 19. an outwardly extending step.

Detailed Description

To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the attached drawings in the embodiments of the present invention are combined to clearly and completely describe the technical solution in the embodiments of the present invention, and obviously, the described embodiments are part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Referring to FIGS. 1-9: the utility model relates to a blood supernatant preparation facilities, include and be used for gathering venous blood and sealed rubber cap 5 to and cooperate with rubber cap 5 and realize inside shell 6 for vacuum environment, the interior upper portion of shell 6 is equipped with the slip shell 4 that is used for separating the supernatant and the ordinary pressure draws the supernatant, and the lower part cover of slip shell 4 is equipped with the interior inferior valve 1 that is used for realizing the blood layering. The utility model realizes a vacuum environment through the matching of the rubber cap 5 and the shell 6, and the vacuum environment has the same function as the existing vacuum blood collection tube so as to be convenient for collecting whole blood by matching with a vein blood collection needle; meanwhile, the utility model is provided with the sliding shell 4 and the inner lower shell 1 inside the shell 6, the cooperation of the two shells is used as a container of whole blood in the process of collecting blood, and is used as a centrifugal container when the blood is centrifugally layered, and is used as a container of physically separating supernatant after the blood is layered (the layered blood is prevented from being in a layered state again), and meanwhile, the blood can be in a normal pressure state when the supernatant is extracted under the cooperation of the shell 6, so as to achieve the characteristic of convenient extraction; based on the above characteristics, the utility model discloses an on the basis that does not change original blood collection form lies in, through using the characteristics that same container was gathered, centrifugation and separation to whole blood, reach the characteristics of being convenient for separate and draw the supernatant under the prerequisite of avoiding blood to pollute.

Further, referring to fig. 1, 2, 5 and 8, the outer wall of the upper side of the sliding shell 4 is provided with an air vent with a bacteria-resistant breathable film 8; the shell 6, the sliding shell 4 and the inner lower shell 1 are all made of transparent materials. The air holes with the bacteria-resistant and breathable films 8 are formed in the outer wall of the sliding shell 4, so that the vacuum and normal-pressure state inside the sliding shell 4 can be conveniently realized, and bacterial pollution and the like in the external environment can be prevented from polluting a blood sample; through shell 6, the slip shell 4 that set up transparent material and interior inferior valve 1 can realize being convenient for observe the volume that whole blood was gathered, the centrifugal separation condition and the purpose of the liquid seal condition of learning.

Further, referring to fig. 1, 4, 5 and 6, the inner side of the top of the sliding shell 4 is connected with the outer wall of the lower portion of the rubber cap 5, a slope-shaped transition surface 9 is arranged on the lower portion of the sliding shell 4, a separation baffle 10 which is of an integrated structure and extends outwards is arranged at the bottom of the slope-shaped transition surface 9, a sealing ring 3 which is matched with the inner wall of the inner lower shell 1 is arranged on the outer side of the joint of the slope-shaped transition surface 9 and the separation baffle 10, the inner side of the joint of the slope-shaped transition surface 9 and the separation baffle 10 is matched with the guide sealing supporting part, and a small groove 11 which is matched with the guide sealing supporting part is formed in the inner side of the joint of the slope-shaped transition surface 9 and the separation baffle 10. The sliding shell 4 in the utility model can move up and down in the inner lower shell 1 so as to separate the layered supernatant and avoid mixing due to shaking; furthermore, the slope-shaped transition surface 9 can be used for conveniently extracting the supernatant, and meanwhile, a foundation is laid for installing the sealing ring 3 and arranging the separation baffle 10; the utility model discloses in separation baffle 10 during the lapse, the mobility of supernatant is better, under the cooperation of separation baffle 10 and sealing washer 3, the supernatant passes through the inside that microgroove 11 got into sliding shell 4 to reach the later stage and be convenient for the purpose of drawing.

Further, referring to fig. 1, 2 and 3, the guiding sealing support part includes a column 12 disposed on the bottom wall of the inner lower shell 1, the column 12 is sleeved with a rubber column 2, and the outer wall of the rubber column 2 is adapted to the inner side of the joint of the slope-shaped transition surface 9 and the separating baffle 10. The utility model discloses in interior inferior valve 1 in be equipped with the stand 12 of taking rubber column 2, above-mentioned structure setting can provide the direction for the junction downstream of slope shape transition face 9 and separation baffle 10 to play sealed interior epitheca 1 and the effect of slip shell 4.

Further, referring to fig. 1 and 7, the rubber cap 5 includes an outer circumferential sealing engagement surface portion, a center groove 13 provided at a center position of a top portion of the rubber cap 5; the outer circumference sealing joint surface part comprises a lower sealing joint surface arranged at the lower part of the outer circumference, the upper part of the lower sealing joint surface is provided with a lower step limiting surface 15, the outer side of the upper part of the lower step limiting surface 15 is provided with an upper sealing joint surface 16, and the upper part of the upper sealing joint surface 16 is provided with an upper step limiting surface 17. The utility model discloses in rubber cap 5 on the one hand and slip shell 4 and shell 6 mutually support and form seal structure, and reach the effect of being convenient for gather whole blood and collecting the supernatant, on the other hand can also provide installation space for helmet 7 to make and form firm structure between helmet 7 and the shell 6.

Further, referring to fig. 1, 5, 7 and 8, the lower sealing joint surface is adapted to the inner wall of the top of the sliding shell 4, a sliding shell boss 14 is arranged on the outer wall of the upper part of the sliding shell 4, and the upper surface of the sliding shell boss 14 is adapted to the lower step limiting surface 15; the upper part of the inner wall of the shell 6 is provided with an outward expanding step 19, the outer wall of the shell 6 corresponding to the upper part of the step 19 is provided with an external thread, the upper combining surface of the step 19 is matched with the lower surface of the sliding shell boss 14, and the inner wall of the shell 6 at the upper part of the step 19 is matched with the upper sealing combining surface 16.

Further, referring to fig. 9, the rubber cap further comprises a protective cap 7 arranged outside the rubber cap 5, a protective cap step 18 is arranged on the inner upper portion of the protective cap 7, an internal thread matched with the external thread on the outer wall of the shell 6 is arranged on the inner wall of the lower portion of the protective cap step 18, and an upper combining surface of the protective cap step 18 is matched with the upper step limiting surface 17. The lower part of the protective cap step 18 of the protective cap 7 limits the shell 6 and is connected with the external thread on the outer wall of the shell 6 through the internal thread to realize the stability of the structure; meanwhile, the upper combining surface of the protective cap step 18 is matched with the upper step limiting surface 17 in the rubber cap 5 to limit the protective cap 7.

Further, referring to fig. 6, the diameter of the small groove 11 is 0.1 to 1mm. The diameter of the small groove in the utility model can be 0.1mm, 0.3mm, 0.5mm, 0.8mm or 1mm, preferably, the edges of two sides of the small groove 11 are matched with the outer surface of the rubber column 2 in the using process, and the two edges are matched to form a gap for passing the supernatant; the diameter of the small groove 11 in the present invention is the diameter if the small groove 11 is circular.

The utility model also provides a preparation method of blood supernatant preparation facilities, this preparation method is: the shell 6 is matched with the rubber cap 5, the interior of the shell is in a vacuum state, a venous blood collection needle penetrates through the rubber cap 5 to realize blood collection, and whole blood enters the inner lower shell 1 and the sliding shell 4 to be incubated and centrifuged to enable the whole blood to be layered; after layering is finished, the inner parts of the sliding shell 4 and the inner lower shell 1 are in a normal pressure state by taking down the outer shell 6; separating the supernatant from the red blood cells after layering by pressing the sliding shell 4 to move downward relative to the inner and lower shells 1; after the separation is finished, the syringe needle is inserted into the rubber cap 5 to extract the supernatant.

The utility model also provides a preparation method of blood supernatant preparation facilities, this preparation method includes following step:

step 1: the inner lower shell 1 is sleeved at the lower part of the sliding shell 4, the outer shell 6 is connected with the protective cap 7 through threads, and finally the rubber cap 5 is pressed and assembled with the upper part of the sliding shell 4, the upper part of the outer shell 6 and the inner side of the protective cap 7; at the moment, the inner lower shell 1 and the sliding shell 4 are in a communicated state, and the inside of the shell 6, the inside of the inner lower shell 1 and the inside of the sliding shell 4 are in a vacuum state under the condition that the rubber cap 5 and the shell 6 form sealing and the ventilation holes with the bacteria-resistant breathable films 8 are matched;

step 2: a medical disposable venous blood sample collecting needle is inserted from the central groove 13 of the rubber cap 5 for vacuum negative pressure blood collection; blood enters the sliding shell 4 and the inner lower shell 1 under the action of negative pressure;

and step 3: incubating and centrifuging after the blood collection in the step 2, wherein blood is layered after the centrifuging, the upper layer is supernatant, and the lower layer is red blood cells;

and 4, step 4: rotating the outer shell 6 to separate the external thread of the outer shell 6 from the internal thread of the protective cap 7, then taking down the outer shell 6, when taking down the outer shell 6, the external air enters the sliding shell 4 after being filtered by the bacteria-resistant breathable film 8, and the inner lower shell 1 and the sliding shell 4 are in a normal pressure state;

and 5: the rubber cap 5 is pressed to enable the sliding shell 4 and the sealing ring 3 to move towards one side of the inner lower shell 1, and when the joint of the slope-shaped transition surface 9 and the separation baffle 10 is in contact with the outer surface of the rubber column 2, the sliding shell 4 is communicated with the inner lower shell 1 only through the small groove 11;

and 6: manually observing the layering condition of blood, and continuously pressing the rubber cap 5 to ensure that the sliding shell 4 continuously descends to the layering position of the supernate and the red blood cells so as to realize the separation of the supernate and the red blood cells; in the pressing process, gas at the upper part of the sliding shell 4 is exhausted through the bacteria-resistant and breathable film 8, so that the rubber cap 5 is prevented from being pressurized;

and 7: the supernatant in the sliding housing 4 is extracted by piercing the disposable sterile syringe needle through the central slot 13.

The utility model can complete the collection of whole blood, the centrifugation of blood, the physical separation of supernatant and the extraction of supernatant in the sliding shell 4 and the inner lower shell 1, so as to avoid the risk of exposing the blood to the external environment; meanwhile, the utility model can realize the characteristics of convenient whole blood collection and supernatant extraction by switching between vacuum and normal pressure, and in order to realize the switching between vacuum and normal pressure, the utility model is provided with the air holes with the bacteria-resistant and air-permeable membrane 8 on the outer wall of the sliding shell 4, so as to prevent the external bacteria from entering and causing the problem of sample pollution during the switching; further, the utility model discloses a descending of slip shell 4 carries out physical isolation to the supernatant, the descending in-process of slip shell 4, separation baffle 10, sealing washer 3 and slope shape transition face 9 and separation baffle 10 make the supernatant pass through the inside that microgroove 11 got into slip shell 4 under the cooperation of junction and rubber column 2, because the diameter of microgroove 11 is the red blood cell entering that 0.1 ~ 1mm can effectively avoid the lower floor to reach and avoid both to take place the problem of mixing again because of rocking. Further, the utility model discloses in set up rubber cap 5, it not only can be convenient for gather blood and draw the supernatant, still for providing vacuum environment and for shell 6, helmet 7 and slip shell 4 provide installation space to firm connection has established the basis between realizing above-mentioned parts.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims (8)

1. A blood supernatant preparation device is characterized in that: the preparation device comprises a rubber cap (5) used for collecting venous blood and sealing, and a shell (6) which is matched with the rubber cap (5) to realize that the interior is a vacuum environment, wherein the inner upper part of the shell (6) is provided with a sliding shell (4) used for separating supernate and extracting supernate under normal pressure, and the lower part of the sliding shell (4) is sleeved with an inner lower shell (1) used for realizing blood layering.

2. A blood supernatant preparation device according to claim 1, wherein: the outer wall of the upper side of the sliding shell (4) is provided with an air hole with a bacteria-resistant air-permeable film (8);

the outer shell (6), the sliding shell (4) and the inner lower shell (1) are all made of transparent materials.

3. A blood supernatant preparation device according to claim 1, wherein: the inner side of the top of the sliding shell (4) is connected with the outer wall of the lower portion of the rubber cap (5), a slope-shaped transition surface (9) is arranged on the lower portion of the sliding shell (4), a separation baffle (10) which is of an integrated structure and extends outwards is arranged at the bottom of the slope-shaped transition surface (9), a sealing ring (3) matched with the inner wall of the inner lower shell (1) is arranged on the outer side of the joint of the slope-shaped transition surface (9) and the separation baffle (10), the inner side of the joint of the slope-shaped transition surface (9) and the separation baffle (10) is matched with the guide sealing supporting part, and a small groove (11) matched with the guide sealing supporting part is formed in the inner side of the joint of the slope-shaped transition surface (9) and the separation baffle (10).

4. A blood supernatant preparation device according to claim 3, wherein: the guide sealing supporting part comprises a stand column (12) arranged on the bottom wall of the inner lower shell (1), a rubber column (2) is sleeved outside the stand column (12), and the outer wall of the rubber column (2) is matched with the inner side of the joint of the slope-shaped transition surface (9) and the separation baffle (10).

5. A blood supernatant preparation device according to claim 3, wherein: the rubber cap (5) comprises an outer circumference sealing joint surface part and a central groove (13) arranged at the center of the top of the rubber cap (5); the outer circumference sealing joint surface part comprises a lower sealing joint surface arranged at the lower part of the outer circumference, the upper part of the lower sealing joint surface is provided with a lower step limiting surface (15), the outer side of the upper part of the lower step limiting surface (15) is provided with an upper sealing joint surface (16), and the upper part of the upper sealing joint surface (16) is provided with an upper step limiting surface (17).

6. A blood supernatant preparation device according to claim 5, wherein: the lower sealing joint surface is matched with the inner wall of the top of the sliding shell (4), a sliding shell boss (14) is arranged on the outer wall of the upper part of the sliding shell (4), and the upper surface of the sliding shell boss (14) is matched with the lower step limiting surface (15);

the upper part of the inner wall of the shell (6) is provided with an outward expanding step (19), the outer wall of the shell (6) corresponding to the upper part of the step (19) is provided with an external thread, the upper joint surface of the step (19) is matched with the lower surface of the sliding shell boss (14), and the inner wall of the shell (6) at the upper part of the step (19) is matched with the upper sealing joint surface (16).

7. A blood supernatant preparation device according to claim 6, wherein: the rubber cap is characterized by further comprising a protective cap (7) arranged outside the rubber cap (5), a protective cap step (18) is arranged on the inner upper portion of the protective cap (7), an internal thread matched with the external thread on the outer wall of the shell (6) is arranged on the inner wall of the lower portion of the protective cap step (18), and the upper combining surface of the protective cap step (18) is matched with the upper step limiting surface (17).

8. A blood supernatant preparation device according to claim 3, wherein: the diameter of the small groove (11) is 0.1-1 mm.

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