CN218550877U

CN218550877U - Tissue culture bag capable of increasing growth rate

Info

Publication number: CN218550877U
Application number: CN202222577712.2U
Authority: CN
Inventors: 唐南昌; 郝代成; 莫光武; 潘柳霖; 尹开虎
Original assignee: Hainan Oncidium Biotechnology Co ltd
Current assignee: Hainan Oncidium Biotechnology Co ltd
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2023-03-03
Anticipated expiration: 2032-09-28

Abstract

The utility model discloses an improve group's tissue culture bag of growth rate, comprises a bag bod, the bag body has cavity and top open-ended rectangle bag in for, the bleeder vent has still been seted up on the front of the bag body or reverse side, bleeder vent department still is equipped with the micropore that closely laminates and separates the fungus ventilated membrane, be equipped with constraint area and bracing piece outside the bag body, each bracing piece passes corresponding constraint area respectively and in order to constitute the braced frame who is used for the support bag body, still be equipped with the pore membrane that hides that is made by transparent plastic film on the bag body, hide pore membrane one end rigid coupling on the front of the bag body, the other end overturns the reverse side that bag body top opening extends to the bag body. Through the tissue culture bag body that sets up the braced frame, shelter from the hole membrane of reserving the aperture and cover the bleeder vent that has the micropore to separate the fungus ventilated membrane, compromise and keep the level and smooth bag face of tissue culture bag, have and clear away water droplet function and ventilative function of separating the fungus to this photic rate and the expiratory rate that improves the interior seed of tissue culture bag improves the growth rate of seed.

Description

Tissue culture bag capable of increasing growth rate

Technical Field

The utility model relates to a plant tissue culture instrument, concretely relates to improve group of growth rate banks up bag with earth.

Background

At present, the method for artificially culturing the oncidium seeds generally comprises the steps of adding a tissue culture medium into a container, putting the oncidium seeds into the tissue culture medium, and finally, placing the tissue culture medium (plant tissue culture medium) under an LED lamp for long-time illumination culture. The main container is a glass bottle, and the tissue culture glass bottle has high light transmittance and high reuse rate, but is expensive in manufacturing cost, troublesome to clean, easy to damage and overhigh in use cost after being synthesized. Therefore, tissue culture bags are favored to be used for seed tissue culture in more planting bases, general tissue culture bags are simple transparent plastic films such as polypropylene single-layer films, and although the tissue culture bags are low in manufacturing cost, convenient for seed cuttage and easy to seal, problems exist in the using process, and the growth of oncidium seeds is not facilitated. Firstly, in the tissue culture process, the tissue culture medium and the plants release water vapor which cannot escape outwards through the tissue culture bag and are gathered into a plurality of water drops with different sizes of dense hemp and are hung on the tissue culture bag, so that the light receiving effect of seeds in the tissue culture bag is influenced; secondly, the tissue culture bag cannot communicate air with the outside in order to block bacteria, and the growth speed of the seeds can be increased if the tissue culture bag is air-permeable, although the growth of the seeds is not influenced; finally, the tissue culture bag is too soft, and can be partially deformed when being touched by people carelessly or after being placed on a shelf for a long time, so that the smooth bag surface is difficult to ensure, the light transmittance is influenced, and the growth of seeds is slowed down.

Therefore, it is necessary to design a tissue culture bag capable of increasing the growth rate, and to solve the above-mentioned three problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an improve group of tissue culture bag of growth rate to solve the problem of description in the background art, through the group that sets up the braced frame can install the tissue culture bag body, shelter from the reservation aperture hide the hole membrane, and cover the bleeder vent that has the micropore to separate the fungus ventilated membrane, compromise and keep the level and smooth bag face of tissue culture bag, have and clear away the water droplet function and breathe freely and separate the fungus function, improve the growth rate that the photic rate and the expiratory rate of seed improve the seed in the tissue culture bag with this.

The technical scheme of the utility model is realized like this:

a tissue culture bag for increasing growth rate comprises a bag body, wherein the bag body is a rectangular bag with a cavity and an opening at the upper part, the rectangular bag is made of a transparent plastic film, air holes are further formed in the front side or the back side of the bag body, two sides of the rectangular bag can be simultaneously or only one side can be simultaneously opened, a microporous bacteria-isolating and breathable film which is tightly attached to the air holes is further arranged at the positions of the air holes, an annular lower binding belt is respectively arranged below four side edges of the bag body, four sides of the rectangular bag body, an annular upper binding belt and two annular lower binding belts are respectively arranged at the positions, close to the same horizontal height of a bag opening, of two side surfaces of the bag body, the upper binding belt and the lower binding belts are made of elastic materials, nine support rods are further arranged on the bag body, the support rods respectively penetrate through the corresponding lower binding belts and the upper binding belts to form a support frame for supporting the bag body, a hole shielding film made of the transparent plastic film is further arranged on the bag body, one end of the hole shielding film is fixedly connected to the front side of the bag body, and the other end of the hole shielding film is turned over the opening above the bag body and extends to the back side of the bag body.

The technical scheme is that the support rods are four transverse support rods, four longitudinal support rods and an upper support rod, the four transverse support rods are arranged at the bottoms of the front side, the back side and the two side surfaces, two ends of each transverse support rod respectively penetrate through a lower binding belt, so that the transverse support rods are enclosed into a rectangular bottom frame, the longitudinal support rods are respectively arranged along the four side edges of the bag body, the lower ends of the longitudinal support rods penetrate through the lower binding belts on the side edges, the upper ends of the longitudinal support rods penetrate through the upper binding belts, so that the longitudinal support rods and the short rods of the rectangular bottom frame at the bottom are enclosed into triangular side frames, the upper support rods transversely penetrate through the two upper binding belts, and the rectangular bottom frame, the two triangular side frames and the upper support rods form a support frame.

The further technical scheme is that the annular inner areas of the upper binding belt and the lower binding belt are larger than the cross sectional area of one supporting rod and smaller than the sum of the cross sectional areas of the three supporting rods.

The further technical scheme is that the hole covering film is a rectangular film, and the center line of the hole covering film is superposed with the center line of the front side of the bag body.

The further technical scheme is that the supporting rod is a glass fiber rod.

The further technical scheme is that the transparent plastic film is a high-temperature-resistant polypropylene single-layer film.

The further technical scheme is that the elastic material is a high-temperature-resistant polypropylene single-layer film.

The beneficial effects of the utility model reside in that:

1. the tissue culture bag has the advantages that the support rods arranged on the tissue culture bag form a stable support frame, certain support force is provided for the tissue culture bag, the tissue culture bag can still keep a smooth bag surface under the action of a small-range external force, and the tissue culture bag is prevented from collapsing. Guaranteed that the seed in the group banks up the bag with earth has better luminousness for a long time to when group banks up the completion back with earth, the bracing piece can be dismantled and get off and use next time.

2. Hide the hole membrane and seal and organize the group and reserve the aperture when the bag through the setting, after group banks up a period of group, group banks up the water vapor of base and plant release and hangs when group banks up the inside water droplet that forms of bag with earth, can turn over and hide the hole membrane, with the slender rod after the disinfection from the internal water droplet with internal surface of bag of deep-going bag of hole department scrape off, take out the slender rod afterwards, seal the aperture once more after hiding the disinfection of hole membrane surface. Not only the small holes are reserved, but also bacteria can not enter the tissue culture bag through the small holes in the tissue culture process.

3. Through setting up bleeder vent and micropore on the group banks up the bag with earth and separate the fungus ventilated membrane, play again and separate the fungus effect when solving the problem that the bag body can not two-way ventilative.

Drawings

Fig. 1 is a three-dimensional structure view of the bag body;

FIG. 2 is a side partial cross-sectional view of the pouch;

fig. 3 is a combined three-dimensional structure view of the bag body and the supporting frame.

In the figure, 1, a lower binding belt, 2, a front surface, 3, a back surface, 4, a side surface, 5, a microporous bacteria-isolating and breathable film, 6, a hole-covering film, 7, an upper binding belt, 8, a bag opening, 9, a tissue culture base, 10, air holes, 11, small holes, 12, a slender rod, 13, a transverse supporting rod, 14, a longitudinal supporting rod, 15 and an upper supporting rod are arranged.

Detailed Description

In order to better understand the technical content of the present invention, the following embodiments are provided, and the present invention is further described with reference to the accompanying drawings.

Referring to fig. 1 to 3, a tissue culture bag for increasing growth rate comprises a bag body, wherein the bag body is a rectangular bag with a cavity therein and an opening at the upper part, and is made of a transparent plastic film, preferably a high temperature resistant polypropylene single-layer film. After being folded, the bottom of the bag body can be a square bottom, two larger rectangular surfaces and two smaller triangular surfaces are formed above the bottom, the two rectangular surfaces of the bag body are defined as a front surface 2 and a back surface 3, the two triangular surfaces are defined as a side surface 4, and the folding position where the front surface 2 or the back surface 3 intersects with the side surface 4 is a side edge. The front surface 2 and the back surface 3 can be simultaneously opened or only one surface is provided with an air vent 10, the air vent 10 is tightly attached with a micropore bacterium isolation breathable film 5, the micropore bacterium isolation breathable film 5 is made of expanded polytetrafluoroethylene material and can be provided with micropores with the average pore diameter of less than 0.22um, invasion pollution of mixed bacterium spores of more than 0.45 micrometers can be isolated, the micropore bacterium isolation breathable film 5 is used for isolating the air vent 10 to be directly contacted with air, and the bacterium isolation effect is achieved while the problem that the bag body cannot be bidirectionally breathable is solved.

The bag is characterized in that a lower binding belt 1 is respectively arranged at the same horizontal height below four side edges of the bag body, the number of the lower binding belts is four, an upper binding belt 7 is respectively arranged at the same horizontal height of two side surfaces 4 of the bag body, which are close to a bag opening 8, the number of the upper binding belts is two, the binding belts are also made of high-temperature-resistant polypropylene single-layer films, and the two ends of each binding belt are welded on the bag body by adopting a high-frequency welding technology, so that the binding belts are in a ring shape.

The tissue culture bag also comprises nine support rods with different lengths for forming a support frame, wherein the support rods are glass fiber rods with the diameter of 1 mm, four transverse support rods 13 are arranged at the bottoms of the front surface 2, the back surface 3 and the two side surfaces 4, two ends of each transverse support rod 13 respectively penetrate through one lower binding belt 1, so that the transverse support rods 13 are encircled to form a rectangular bottom frame, four side edges of the bag body are respectively provided with one longitudinal support rod 14, the lower end of each longitudinal support rod 14 penetrates through the lower binding belt 1 on the side edge, the upper end of each longitudinal support rod 14 penetrates through the upper binding belt 7, so that the longitudinal support rods 14 and short rods of the rectangular bottom frame at the bottom are encircled to form a triangular side frame, and one upper support rod 15 transversely penetrates through the two upper binding belts 7.

Specifically, the annular inner areas of the upper and

lower straps

7 and 1 are larger than the cross-sectional area of one support rod and smaller than the sum of the cross-sectional areas of the three support rods, and when the upper or

lower strap

7 or 1 is simultaneously penetrated by the three support rods, the three support rods are bound and fixed by the tensile force formed by pulling and deforming the upper or

lower strap

7 or 1. In order to strengthen the fixation, glue, such as 502 glue, can be dripped on the joint of the three support rods.

This group banks up bag with earth still includes the hole membrane 6 that hides of welding on 8 below bag body openly 2 of sack through high frequency welding technique, hide hole membrane 6 by the rectangle film of making with the same material of the bag body, hide the central line of hole membrane 6 and the central line coincidence of the positive 2 of the bag body, be 1 millimeter with a thickness when sealing for the bag body, the width is 2 millimeters, the cross section is the stainless steel slender pole 12 of rectangle and gos deep into the middle part of sack 8, then put into the capper with sack 8 in the mouth that seals, extract slender pole 12 after finishing sealing, a aperture 11 has just been reserved in the department of sealing, will hide hole membrane 6 and overturn sack 8 to reverse side 3 this moment, reuse transparent adhesive will hide hole membrane 6 and fix at reverse side 3, this hides hole membrane 6 and covers aperture 11 completely, prevent that the dust from aperture 11 department from falling into the internal portion of bag. When the small water drops are collected on the inner surface of the bag body, the hole-shielding film 6 is turned over, and the sterilized slender rod 12 is used for penetrating into the bag body from the small hole 11 to scrape the water drops on the inner surface of the bag body.

The utility model discloses the theory of operation:

when the tissue culture bag is used, the tissue culture medium 9 and the oncidium cuttage seeds are firstly filled in the bag body, then the method in the text is installed for sealing, the supporting rod is assembled according to the method in the text after the sealing is finished, and the tissue culture bag can be placed under an LED and the like for tissue culture after the assembly is finished.

After tissue culture is carried out for a period of time, water vapor released by the tissue culture medium 9 and the plants is hung in the tissue culture bag to form water drops, the hole covering film 6 is turned over, the sterilized slender rod 12 extends into the bag body from the small hole 11 to scrape the water drops on the inner surface of the bag body, then the slender rod 12 is drawn out, and the small hole 11 is sealed again after the surface of the hole covering film 6 is sterilized.

The microporous bacteria-isolating breathable film 5 on the tissue culture bag has the effect of isolating bacteria while solving the problem that the bag body cannot be bidirectionally breathable.

The bracing piece on the group banks up the bag with earth has formed comparatively stable support, has provided certain holding power for group banks up the bag with earth, has guaranteed to group and has banked up the bag still can keep smooth bag face under the exogenic action of minim scope with earth, has also prevented group to bank up the bag self and has subsided. After the tissue culture is completed, the supporting rod can be detached to be used next time, and a small amount of glue on the supporting rod can be cleaned by the glue dissolving agent and can be removed.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an improve group of growth rate banks up bag with earth, includes the bag body, and the bag body is for interior cavity and top open-ended rectangle bag, is made by transparent plastic film, has still seted up the bleeder vent on the front of the bag body or reverse side, and both sides can be trompil simultaneously or only a trompil, and the micropore that bleeder vent department still was equipped with inseparable laminating separates fungus ventilated membrane, its characterized in that: the bag body is characterized in that an annular lower binding belt is respectively arranged below four side edges of the bag body, the number of the lower binding belts is four, an annular upper binding belt is respectively arranged at the position, close to the same horizontal height of the bag opening, of two side surfaces of the bag body, the two side surfaces of the bag body are two, the upper binding belt and the lower binding belt are made of elastic materials, nine support rods are further arranged on the bag body, each support rod penetrates through the corresponding lower binding belt and the corresponding upper binding belt respectively to form a support frame for supporting the bag body, a hole shielding film made of a transparent plastic film is further arranged on the bag body, one end of the hole shielding film is fixedly connected to the front surface of the bag body, and the other end of the hole shielding film is turned over an opening above the bag body and extends to the back surface of the bag body.

2. The tissue culture bag for increasing growth rate of claim 1, wherein: the support rods are four transverse support rods, four longitudinal support rods and one upper support rod respectively, the four transverse support rods are arranged at the bottoms of the front side, the back side and the two side faces, two ends of each transverse support rod respectively penetrate through a lower binding belt, so that the transverse support rods surround a rectangular bottom frame, the longitudinal support rods are respectively arranged along the four side edges of the bag body, the lower ends of the longitudinal support rods penetrate through the lower binding belts on the side edges, the upper ends of the longitudinal support rods penetrate through the upper binding belts, so that the longitudinal support rods and the short rods of the rectangular bottom frame at the bottom surround triangular side frames, the upper support rods transversely penetrate through the two upper binding belts, and the rectangular bottom frame, the two triangular side frames and the upper support rods form a support frame.

3. The tissue culture bag for increasing growth rate according to claim 2, wherein: the annular inner areas of the upper binding belt and the lower binding belt are larger than the cross sectional area of one supporting rod and smaller than the sum of the cross sectional areas of the three supporting rods.

4. A tissue culture bag for increasing growth rate according to claim 3, wherein: the hole covering film is a rectangular film, and the center line of the hole covering film is superposed with the center line of the front side of the bag body.

5. A tissue culture bag for increasing growth rate according to claim 4, wherein: the support rod is a glass fiber rod.

6. A tissue culture bag for increasing growth rate according to any one of claims 1-5, wherein: the transparent plastic film is a high-temperature-resistant polypropylene single-layer film.

7. The tissue culture bag for increasing growth rate according to claim 6, wherein: the elastic material is a high-temperature-resistant polypropylene single-layer film.