JP2002084884A - System for inoculating mushroom such as cortinellus shiitake, lyophyllum aggregatum or ganoderma lucidum, and method for culturing mushroom by use of the inoculation system - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve the problems, such as the special structure of a plastic film bag, high cost, and complicated handlings, of a conventional structure for using a culture medium in an air-permeable and sterilized bag having a special structure, inoculating the culture medium with the spawns of a mushroom and then culturing the spawns, thus stimulating the spreading of the hyphae and the formation of the anlagen and comprehensively shortening a culture period to improve the efficiency of the culture, wherein the shortening of the culture period is furthermore desired, because about one month is still needed, although the period is shortened, and further to solve the problems, such as the specific structures of bar spawns, high cost, complicated handlings, and the lack of generality, of a proposed simplified culture method using a bag. SOLUTION: This system for inoculating the mushroom, comprising charging and sealing many toothpicks 3 in a sterilized air-permeable bag 1, inserting a fruit body piece 4 to inoculate the fungi of the mushroom, and then culturing the mushroom. Thereby, the mushroom can be produced in an extremely short period. The mushroom can simply and surely be produced.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mushroom seeding system for mushrooms, such as shiitake mushrooms, shrimp mushrooms, and mushrooms, and a method for cultivating mushrooms using the seeding system.

[0002]

2. Description of the Related Art Conventionally, prior art documents relating to a mushroom seeding system for mushrooms and a method for cultivating mushrooms using the seeding system include the following documents 1-3.

[0003] First, as a mushroom seeding system for mushrooms,
There is a mushroom cultivation bag of No. 6-141677 and a mushroom cultivation method using the bag (Reference 1). The content of this document 1 is to form a temporary bottom in a plastic film-made mushroom cultivation bag having a gas-permeable bottom filter near the bag bottom and a gas-permeable bag mouth filter near the bag mouth and filling the medium. After sterilization and sealing of the bag mouth, the seed medium is inoculated from the outside at almost the center of the culture medium, and the culture is carried out in a state that can be aerated from the bottom and the bag mouth filter. And shorten the cultivation period comprehensively to improve cultivation efficiency.

[0004] Next, as a method for cultivating mushrooms using a seeding system, a method for cultivating mushrooms disclosed in Japanese Patent Application Laid-Open No. 8-294326 (Reference 2) and a method using a rod-type fungus disclosed in Japanese Patent Application Laid-Open No. 9-23745 and a method using the same were used. There is an inoculation method (Reference 3). The content of this document 2 is that the solid medium is sealed in an air-tight film bag and sterilized, and then a rod-shaped inoculum or wood flour or a seed cultivated by inoculating a mushroom fungus into a grain is filled in the bag. In a method of cultivating mushrooms having a configuration in which a seed is cultured using a vent provided in the bag and a light-shielding film is fixed to a predetermined portion such as the upper surface of the bag to generate fruit body pieces. Yes, cultivation method of mushrooms, using a simple medium that is sterilized and reliably sterilized to prevent the propagation of various germs, or cultivation method of high commercial value mushroom, easy cultivation method of harvesting work In the offer. The content of Document 3 is a configuration in which the inoculum is purely cultured on a rod-shaped member having one end sharpened. Therefore, since the inoculum reaches the deep part of the planting ground by piercing the planting ground, the use amount of the inoculum can be significantly reduced. Also, since the seeds are inoculated by piercing the seeds of the sticks without opening the bag, the seeds can be inoculated with the seeds without disinfecting them. Inoculation of the inoculated fungi can be performed throughout the year. In addition, since the inoculation operation is extremely simple, even a beginner can easily perform the inoculation operation.

[0005]

The document 1 mentioned above can shorten the period required for producing the original bacteria. However, there is a problem that the bag made of a plastic film has a special structure, the cost is high, and the handling is complicated. In addition, it takes approximately one month even if the number of days decreases, so further shortening is desired.

[0006] In References 2 and 3, seeding in a sterile room can be avoided. However, there is a problem that the composition of the rod inoculum (rod-shaped inoculum) has a special structure, which increases the cost, is complicated in handling, and lacks versatility. In addition, since an appropriate structure is not adopted in the adhesion and fixability between the stick-type bacterium and the bag, there is a possibility that the stick-type germ may fall off, which is a problem.

[0007]

Means for Solving the Problems The invention of claim 1 intends that the original bacteria can be produced in a very short time (about 2 weeks), and that the original bacteria can be produced simply and reliably.

According to the first aspect of the present invention, a large number of toothpicks are put in a sterilized air-permeable bag and sealed, and the fruiting body is inserted into the sealed bag to seed the fungi. Is a mushroom seeding system configured to generate a mushroom.

The invention of claim 2 provides a concrete example for achieving the invention of claim 1.

The second aspect of the present invention is that water containing nutrients such as rice bran,
After putting a lot of toothpicks and boiling for about 10 minutes, drain the water from these lots of toothpicks and put the toothpick into a breathable thickness of 0.01 mm.
It is put in a polyethylene bag, sealed, boiled for about 10 minutes, sterilized by boiling, and then cooled to produce a medium for collecting the original bacteria from natural fruiting body pieces. Approximately 1 cm in the medium of this polyethylene bag
This is a mushroom mushroom seeding system configured to generate the original fungus after about 2 weeks at a temperature of about 25 to 30 degrees after inserting and sealing the fruit body pieces of the horn.

[0011] The invention of claim 3 is to produce mushrooms reliably and simply by using the original fungus seeded at an appropriate time, and to produce mushrooms without using a special room (base) and base material. It is intended to be easily generated.

According to a third aspect of the present invention, a log having a seeding portion is placed in a bag having air permeability, the log is sterilized by boiling with the bag, the bag is hermetically sealed, and the bag is provided with inclusions such as sponge. The toothpick with the inoculum is stabbed into the bag via the inclusions, the toothpick with the inoculum is provided in the seeding portion of the sterilized raw wood, and the raw wood with the toothpick is temporarily laid down and buried in the ground. This is a method for cultivating mushrooms using a seeding system that is configured to be overturned.

[0013] The invention of claim 4 relates to the adhesion between the toothpick and the bag.
To secure the fixation, avoid the invasion of various bacteria and prevent the toothpick from falling off.

According to a fourth aspect of the present invention, there is provided a method for cultivating a mushroom using a seeding system in which an inclusion and a toothpick are adhered to each other, and the inclusion and the toothpick are sealed and fixed.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION A mushroom seeding system for shiitake mushrooms, shrimp mushrooms, mushrooms and the like (hereinafter referred to as mushrooms) shown in FIGS. 1 to 7 will be described.

The present invention is directed to natural fruiting body pieces (eg, Reishi)
A mushroom seeding system will be described in which the original bacteria are collected without using any sterile room and no chemicals, and the fruiting body pieces are reliably cultivated by culturing.
The fruiting body pieces are collected from forests and mountains in the desired size and form. The collected fruiting body pieces are thoroughly washed with water to remove dirt, wipe off moisture with a cloth or the like, and stored indoors. Then, a pot or vessel (hereinafter referred to as a pot) is prepared, and an appropriate amount of water is put in the pot and boiled (the size of the pot is determined according to the production amount of the original bacteria).

An appropriate amount of nutrients such as rice bran and a commercially available toothpick are put into the pot, and after boiling, boiled for about 10 minutes to impregnate the nutrients. After boiling, raise it to saul, drain excess water, spread it on newspapers, etc., and while it is still water, a commercially available polyethylene bag with a thickness of about 0.01 mm (other bags may be used).
50-100 pieces (the number is not limited to this, but this seems to be an appropriate amount) and a resin pipe (4cm in diameter and 7cm in length)
Do not close the mouth of the bag together (to prevent air inflation or make it easier to sterilize, etc.) without putting the mouth of the bag together. Boil for about 10 minutes with the lid closed to complete sterilization. In addition, the direction of the toothpick of the bag is aligned to facilitate the subsequent inoculation, and the head is aligned in a predetermined direction.

When the sterilization is completed, quickly close the bag by closing the mouth of the bag, etc., and keep the pipe upright while it is hot, fix it with a ring comb from the outside (see Fig. 1) and expose it to the outside air. This is because it is easy to use the expansion of air.) This is the medium from which the original bacteria are collected from the natural fruiting bodies.

Thereafter, the bag containing the toothpick and the tea strainer (filtering and scooping device) are placed in boiling water. The bag is cut from the position indicated by a dashed line (A is a cutting position). That is, a very small portion is cut with scissors or the like. At this time, with the mouth of the pipe kept in hot water, stick out the ring comb (tightening body) fixing the pipe with care so as not to remove it (see FIG. 4).

In the right place of the above-mentioned fruiting body (the right place of umbrella, leg, etc.), 1c
Cut into square meters, support the fruiting body pieces with chopsticks and rinse in hot water (this rinsing function serves to sterilize the adhesion of various bacteria due to the outside air), and quickly release under the pipe, The pieces of the fruit body float up to the water level in the pipe and enter the pipe. Then, a tea strainer is applied from under the pipe, and the tea strainer is raised carefully so as not to separate the tea strainer and the pipe. Put the pieces in a bag using a pipe. Perform this operation for about 5 seconds, and confirm that the fruiting body pieces have fallen into the bag. Quickly twist the lower part of the pipe to remove the pipe, tie it tightly, and seal it. In addition, bacteria generation could be confirmed in 3 to 5 days at room temperature and air temperature of about 25 to 30 degrees. Therefore, about
In two weeks (about 14 and 15 days), the bacteria turn around and serve as a guide to the formation of the original bacteria (for the working process, see FIGS. 6 (a) to 6 (l)).
Incidentally, to explain the main part of FIG. 6, (c) is a diagram of cutting a bag,
(E) Rinsing the fruiting body pieces, (F) Drawing the fruiting body pieces in a pipe, (G) Drawing the fruiting body pieces in a pipe, (R)
Shows a figure in which the fruiting body pieces enter the bag, (nu) shows a figure in which the fruiting body pieces fall into the bag, and (lu) shows a figure in which the bag containing the fruiting body pieces is tied.

When the original microorganism is completed, the original microorganism is further cultured to produce a required amount of the original microorganism in order to inoculate the raw wood. For example, it is made using a toothpick medium. In this toothpick medium, the toothpick is boiled and sterilized, and the air in the bag is quickly evacuated while hot, and the mouth is tied and sealed (see FIG. 5).
The humidity of this sterilized toothpick medium is about 65%. Seed the sterilized toothpick soaked with this nutrient. That is, a toothpick with an inoculum (the inoculum is regenerated in the toothpick through an inclusion (such as an approximately 2 cm square, and preferably about 3 mm or more in thickness, the inclusion is preferably cut into about a 2 cm square)) such as a foam and a tape in the bag. ) And a large number of original bacteria are cultured (see FIG. 7). The bag and the toothpick are fixed through the inclusion, and the germs attached to the outside of the toothpick (hereinafter referred to as germs adhering to the toothpick) through the inclusion are peeled off so as not to enter the bag. Normally, the temperature is about 25 degrees
At 30 degrees, it is completed in about 7 to 10 days and becomes a seed for culture and cultivation of raw wood (sawdust, planting ground, etc.).

A method for cultivating the mushrooms shown in FIGS. 8 to 17 will be described. According to the present invention, mushrooms are produced using a seed fungus by toothpick culture. The log is cut to about 15-20cm, put in a bag, and a ring comb is attached at the approximate center of the log to fix the bag to the log. This log is dipped in boiling water together with the bag. At this time, do not close the mouth of the bag (however, do not allow hot water to enter. It is better to bleed the air from the bag), and then close the lid of the pot. For example, when the diameter of the raw wood is about 15 to 20 cm, it is sterilized by boiling for about 1 to 2 hours. When the boiling sterilization is completed, close the bag quickly by closing it, etc. (At this time, try to remove as much air from the bag as possible), and wait for it to cool to room temperature.

When the sterilized log in the bag is cooled, a toothpick with a seed is inserted into the sterilized log in the bag via an inclusion. For example, as shown in FIG. 13 (c), a toothpick with an inoculum is inserted into the hole of the log. The number of stabs is, for example, about 1 to 6 and is not limited. Further, as shown in FIG. 14 (c), it contacts at least the upper surface of the log. In any case, it is important to insert a toothpick with seeds into the raw wood or to attach it so that it always touches at least the upper surface of the raw wood. At about 25-30 degrees Celsius, the hypha spreads on the raw wood in about one month, when the hyphae are found to be active on the wood mouth in about 3-5 days. It is also possible to seed the side of the log (not shown).

[0024] The purpose of using the above-mentioned inclusions is that when the toothpick with the inoculum is taken out of the bag, it comes into contact with the outside air, so that various bacteria may adhere to the outside of the toothpick. Therefore, by passing the inclusions, various bacteria on the outside of the toothpick can be removed, and the contact portion is fixed and the outside air can be shut off without moving. Therefore, the sterile room,
It is beneficial and simple because there is no need to use any drugs and there is no need to select a place.

The seeded logs are piled up at an appropriate height as shown in FIG. 17 until they are temporarily laid down at the natural temperature. There is no need to spray or apply medicine. In addition, it is possible to easily inoculate the culture without using any sterile room medicine or the like, or to grow a fruiting body piece that can surely spread the bacteria.

In the case of the bookbinding, all the bags are removed and planted in soil. In addition, if it is an average year, buds will begin to be blown around May and the harvest can be completed by July.

The advantages of the toothpick are that it is commercially available at a low cost, is easily available at a low cost, is easy to handle without being bulky in quantity, and can shorten the sterilization time. There are advantages such as what can be done before and after. There is also the benefit of being able to inoculate quickly and accurately. To sum up, toothpick
The use of the method of the present invention, using a toothpick with inoculum and using a bag and boiling, etc., makes it possible to use a sterile room, no medicine at all, and to be able to do it with bare hands without specifying the place, easily and quickly. There are features such as what can be done.

[0028]

An embodiment of the present invention will be described below.

In the figure, 1 is a bag, 2 is a pipe, 3 is a toothpick, 4 is a piece of fruit body, 5 is a net, and 6 is a ring comb.
Put several toothpickes in the pan and boil in pan 7. The bag 1 sterilized by this boiling is sealed using a ring comb 6. 8 in the figure is water level, 9
Denotes a heating means, and 9a denotes a heat shield plate.

In the figure, 10 is a log, 10a is a hole in the log 10, 11 is a bag, 12 is a ring comb, 13 is a toothpick with an inoculum, 14 is an inclusion, 15
Indicates a pot and 16 indicates a lid. Put the raw wood 10 in this pot 15 and boil,
Sterilize. The bag 11 is prevented from entering hot water, and the bag 11 is put into the pan 15 so that air is evacuated. After sterilization, it is sealed with a ring comb 12. This sealed log 10
A few toothpicks with inoculum 13 are stabbed through the inclusions 14,
Seeding. With this seeding, hyphae spread to the log in about a month. After that, they are piled up at an appropriate height until they are temporarily laid down at the natural temperature.

[0031]

According to the first aspect of the present invention, a large number of toothpicks are put in a sterilized air-permeable bag and sealed, and the fruiting body pieces are inserted into the sealed bag to seed the fungi. And a mushroom seeding system for producing mushrooms. Therefore,
There are features such as the ability to generate the original bacterium in a very short time, and the ability to easily and reliably generate the original bacterium.

According to the invention of claim 2, a large number of toothpicks are put in water containing nutrients such as rice bran and boiled for about 10 minutes. Some thickness
Place in a 0.01mm polyethylene bag, seal, boil for about 10 minutes, sterilize by boiling, and then cool to produce a medium for collecting the original bacteria from the natural fruiting body. In the medium of this polyethylene bag,
After inserting a 1 cm square fruit body piece and sealing it, the temperature is about 25 degrees to 3 degrees.
This mushroom mushroom seeding system is configured to generate the original bacteria at about 0 degrees after about 2 weeks. Therefore, a specific configuration for achieving the invention of claim 1 can be provided.

According to the invention of claim 3, the raw wood having the seeding portion for seeding the original fungus is sterilized by boiling, the sterilized raw wood is put in a bag having air permeability and sealed, and the bag is filled with inclusions such as foam. Insert a toothpick with inoculum through, insert the toothpick into the seeding part,
This is a method for cultivating mushrooms using a seeding system in which a raw wood with a toothpick is temporarily laid down, tentatively laid down, and then buried in the ground except for a bag and fully laid down. Therefore, there is a feature that the mushroom is reliably and simply generated by using the original fungus seeded at an appropriate time, and that the mushroom is reliably and simply generated without using a special room or base material. .

The invention of claim 4 is a method for cultivating a mushroom using a seeding system in which an inclusion and a toothpick are adhered to each other and the inclusion and the toothpick are sealed and fixed. Therefore, there is a feature that the adhesion and the fixability between the toothpick and the bag are ensured to prevent invasion of various bacteria and prevent the toothpick from falling off.

[Brief description of the drawings]

FIG. 1 is an example of a mushroom seeding system, and is a schematic diagram showing a relationship between a bag, a toothpick, a lip, and the like.

FIG. 2 is an example of a mushroom seeding system, and is a schematic diagram illustrating a relationship between a pan and a heating unit.

FIG. 3 is an example of a mushroom seeding system, including a pot, a bag, a toothpick,
FIG. 3 is a schematic diagram showing a relationship between the data and a pipe.

FIG. 4 is an example of a mushroom seeding system, and is a schematic diagram showing a relationship between a bag and a pipe.

FIG. 5 is an example of a mushroom seeding system, and is a schematic diagram showing a state in which many boiled and sterilized toothpicks are put in a bag.

FIG. 6 is an example of a mushroom seeding system, showing a process of producing original bacteria, (a) is a schematic diagram showing an example of a pan, and (b) is a schematic diagram of immersing a mouth of a bag with a toothpick in boiling water. Figure, (c) is a schematic diagram of cutting a bag in boiling water, (d) is a schematic diagram of putting fruiting body pieces in boiling water, (e) is a schematic diagram of rinsing fruiting body pieces, (f)
Is a schematic diagram of putting a fruiting body piece in a pipe, (g) is a schematic view of a fruiting body piece in a pipe, (h) is a schematic view illustrating the relationship between a fruiting body piece and a toothpick in a bag and a tea strainer, (R) is a schematic diagram of a fruiting body piece entering a bag, (N) is a diagram of a fruiting body piece falling into a bag, (R)
FIG. 4 is a schematic view showing a state in which a bag containing a fruiting body piece is tied.

FIG. 7 is an example of a mushroom seeding system, in which (a) a schematic diagram showing a bag with a seeded toothpick, (b) a schematic diagram showing a toothpick with a seed fungus, and (c) through an inclusion. It is a schematic diagram which shows the state which stabs a toothpick with a seed fungus in a bag.

FIG. 8 is an example of a method for cultivating mushrooms, showing each example of a raw wood, and (a) is a schematic view of a partially cut raw wood having holes.
(B) is a schematic diagram showing an example of using a raw wood as it is.

FIG. 9 is an example of a method for cultivating mushrooms, and is a schematic diagram showing a state in which a log is put in a bag.

FIG. 10 is an example of a method for cultivating mushrooms, and is a schematic view of a partially cut-out state in which a bag containing raw wood is put in a pot and boiled.

FIG. 11 is an example of a method for cultivating mushrooms, and is a schematic view of a partially cut-out state of a state in which boiling of a log-containing bag placed in a pot is completed.

FIG. 12 is an example of a method of cultivating mushrooms, and is a schematic view of a partially cut-out state of a log in a bag at room temperature.

FIG. 13 is an example of a method for cultivating mushrooms, showing a process of sticking a toothpick with a seed fungus into a raw wood, and (a) shows a state in which an inclusion is provided at an appropriate place (near a hole) of a bag. (B) is an enlarged schematic view of a toothpick with inoculum showing the preparation stage, and (c) is a partially omitted schematic view showing a state where the toothpick has been inserted into the bag via an inclusion. FIG. 2D is an enlarged schematic cross-sectional view of a main part of FIG.

FIG. 14 is an example of a method for cultivating mushrooms, showing a process of stabbing a toothpick with a seed fungus on a raw wood, and (a) schematically showing a preparation stage in a state where an inclusion is provided in an appropriate place of a bag. Figure, (b)
Is a schematic diagram showing a state of being inserted into the bag via an inclusion,
(C) is an enlarged schematic diagram of a main part.

FIG. 15 is a schematic diagram showing another example of a method for cultivating mushrooms, in which a toothpick with a seed fungus is inserted into a raw wood.

FIG. 16 is an example of a method for cultivating mushrooms, and is a schematic view of FIG. 15 as viewed from a bird's eye.

FIG. 17 is an example of a method for cultivating mushrooms, and is a schematic scale diagram showing a state in which inoculation is completed and raw wood is temporarily laid down.

[Explanation of symbols]

 1 bag 2 wire 3 toothpick 4 fruit body pieces 5 net 6 wheel comb 7 pot 8 water level 9 heating means 9a heat shield 10 log 10a hole 11 bag 12 wheel comb 13 toothpick with seeds 14 inclusions 15 pot 16 lid

Claims (4)

[Claims] [1] A large number of toothpicks are put in a sterilized air-permeable bag and sealed, and a fruiting body is inserted into the sealed bag to seed the fungi to produce the original fungus. A mushroom seeding system for shiitake mushrooms, shimeshi mushrooms, mushrooms, etc. 2. A large number of toothpicks are put in water containing nutrients such as rice bran and boiled for about 10 minutes. Then, the large number of toothpicks are drained, and the toothpick is permeable to a thickness of about 0.01 mm. Put in a polyethylene bag, seal it, boil it for about 10 minutes, sterilize it by boiling, and then cool it to produce a medium for collecting the original bacteria from the natural fruiting body pieces. After inserting and sealing about 1 cm square fruit body pieces in the medium of this polyethylene bag and sealing, after about two weeks at a temperature of about 25 to 30 degrees Celsius, Shiitake mushroom, Shimesi, Mannentake, etc. Mushroom seeding system. 3. A raw wood having a seeding portion is placed in a bag having air permeability, the raw wood is sterilized by boiling with the bag, the bag is sealed, and an intervening material such as a foam is provided in the bag. The toothpick with the inoculum is stabbed into the bag through a material, and the toothpick with the inoculum is provided in the seeding portion of the sterilized raw wood. A method for cultivating mushrooms using a configured seeding system. 4. The method for cultivating a mushroom using the seeding system according to claim 3, wherein the inclusion and the toothpick are bonded to each other, and the inclusion and the toothpick are sealed and fixed.