CN218465843U - Phage culture apparatus - Google Patents
Phage culture apparatus Download PDFInfo
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- CN218465843U CN218465843U CN202222934690.0U CN202222934690U CN218465843U CN 218465843 U CN218465843 U CN 218465843U CN 202222934690 U CN202222934690 U CN 202222934690U CN 218465843 U CN218465843 U CN 218465843U
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Abstract
The utility model discloses a phage culture apparatus belongs to microorganism culture apparatus technical field, and the easy technical problem who pollutes, is difficult to cultivate the bacterial strain in a large number of culture medium of having solved prior art existence, and concrete technical scheme is: the utility model provides a phage culture apparatus, the power distribution box comprises a box body, a plurality of shielded boxes have been arranged in the box, divide into first cavity and second cavity through the baffle in the shielded box, the culture dish has been arranged in the first cavity, the double-ported flask has been arranged in the second cavity, the bottom of culture dish is equipped with unloads the liquid mouth, it is sealed that the liquid mouth passes through the silica gel stopper to unload, the middle part of silica gel stopper is equipped with the water conservancy diversion passageway, the water conservancy diversion passageway is sealed through elasticity rubber buffer, the first bottleneck of double-ported flask is linked together through honeycomb duct and silica gel stopper, staphylococcus aureus cultivates in the culture dish of independently cutting apart, guarantee that whole culture process operates under aseptic environment, staphylococcus aureus purification separation and transition are bred to the double-ported flask in the culture dish, staphylococcus aureus can propagate in a large number.
Description
Technical Field
The utility model belongs to the technical field of the microorganism culture apparatus, concretely relates to device of bacterial strain is cultivateed in disconnect-type purification.
Background
Staphylococcus aureus is a common food-borne pathogenic microorganism, which is most preferably grown at 37 ℃ and pH7.4, is resistant to high salt, can grow in an environment with salt concentration close to 10%, is commonly parasitic on skin, nasal cavity, throat, intestines and stomach, carbuncle and pyogenic wound of human and animals, and is also abundantly present in air and sewage environment. In order to better research the staphylococcus aureus, more pure staphylococcus aureus needs to be cultured, and an optimal device for culturing the staphylococcus aureus needs to be researched.
Most of common culture dishes on the market can not integrate purification and mass propagation, the culture of bacteria is isolated by multiple steps and multiple facilities, and culture equipment is easy to cause pollution, so that the cultured strains are impure. Because the space of the common culture box on the market is limited, the cultured strains are less, the time consumption is long, the consumption is high, the requirement of mass propagation of the strains cannot be realized, and the utilization rate is low.
SUMMERY OF THE UTILITY MODEL
For solving the technical problem that prior art exists, the utility model provides a cultivation instrument of bacteriophage, aseptic technique can be realized to this culture dish, can realize the independent culture of bacterial strain, realizes the high-efficient reproduction of bacterial strain.
In order to achieve the above object, the utility model adopts the following technical scheme: the utility model provides a phage culture apparatus, includes the box, according to the inner space of box, has arranged a plurality of shielded cells in the box, and every shielded cell is all independent, and every independent shielded cell all can cultivate the bacterium. The inside of the isolation box is divided into a first cavity and a second cavity by a partition plate, the first cavity is arranged above the second cavity, and the height of the first cavity is smaller than that of the second cavity.
The culture dish has been arranged in the first cavity, and the middle part of first cavity is arranged in to the culture dish, and the culture dish is used for cultivateing the bacterium, and the cultivation of bacterium can be accomplished to the inoculation in the culture dish once, arranges ultraviolet lamp in the first cavity and can disinfect the disinfection to first cavity.
A double-mouth flask is arranged in the second cavity, the bottom of the double-mouth flask is placed on the bottom plate of the second cavity, a first bottle mouth of the double-mouth flask is arranged in parallel with the second bottle mouth, and the first bottle mouth is arranged below the culture dish.
The bottom of culture dish is equipped with unloads the liquid mouth, unloads the liquid mouth and seals through the silica gel stopper, and the middle part of silica gel stopper is equipped with the water conservancy diversion passageway, and the water conservancy diversion passageway is sealed through elastic rubber stopper, and the first bottleneck of two-mouth flask is linked together through honeycomb duct and silica gel stopper. When the bacteria are cultured in the culture dish, the elastic rubber plug is inserted into the silica gel plug, the bacteria are cultured in the culture dish, after the culture is finished, the elastic rubber plug is pulled up, and the liquid culture medium flows into the double-mouth flask after passing through the flow guide channel and the flow guide pipe, so that the next step of culture separation can be carried out.
The bottom of box is equipped with attemperator, and attemperator comprises temperature sensor and the electric heating wire of arranging in the box, and temperature sensor is used for monitoring the temperature in the box, crosses when low when the temperature in the box, and electric heating wire work can rise the temperature in the box to satisfy the temperature demand that the bacterial strain was cultivateed.
The box body is square, and the number of the isolation boxes in the box body is four.
The lateral surface coating of box has heat insulation reflecting layer, and heat insulation reflecting layer is evenly paintd by heat-reflecting material and is formed to improve the heat preservation effect of whole box.
Compared with the prior art, the utility model, specifically beneficial effect is embodied in:
1. the utility model discloses a be divided into a plurality of shielded boxes with the box, the culture medium of solid cultivates in the culture dish, forms the culture solution after the cultivation is accomplished to the mode that solid/liquid conversion combines, staphylococcus aureus cultivates in the culture dish of independently cutting apart, guarantees that whole cultivation process is operated under aseptic environment, staphylococcus aureus purification separation and transition in the culture dish cultivates the breed to two mouthfuls of flasks, and is easy and simple to handle, avoids many times to change culture dish and causes the pollution to the culture solution.
2. The utility model discloses a accuse temperature and four culture dish devices let staphylococcus aureus can propagate in a large number, and the bacterial high-usage, the cultivation is efficient.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the isolation box in fig. 1.
FIG. 3 is a schematic diagram showing the connection between a petri dish and a double-neck flask.
In the figure, 1 is a box body, 2 is an isolation box, 3 is a first chamber, 4 is a second chamber, 5 is a culture dish, 6 is a double-mouth flask, 7 is a first bottle mouth, 8 is a second bottle mouth, 9 is a liquid discharging port, 10 is a silica gel plug, 11 is an elastic rubber plug, 12 is a flow guide pipe, and 13 is a temperature adjusting device.
Detailed Description
In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1 to 2, a bacteriophage cultivating apparatus includes a case 1, a plurality of isolation boxes 2 are arranged in the case 1 according to an inner space of the case 1, each isolation box 2 is independently arranged, and each independent isolation box 2 can cultivate bacteria. The inside of the isolation box 2 is divided into a first chamber 3 and a second chamber 4 by a partition plate, the first chamber 3 is arranged above the second chamber 4, and the height of the first chamber 3 is smaller than that of the second chamber 4.
A double-neck flask 6 is arranged in the second chamber 4, the bottom of the double-neck flask 6 is placed on the bottom plate of the second chamber 4, a first bottleneck 7 of the double-neck flask 6 is arranged in parallel with a second bottleneck 8, and the first bottleneck 7 is arranged below the culture dish 5.
The bottom of the culture dish 5 is provided with a liquid discharging port 9, the liquid discharging port 9 is sealed by a silica gel plug 10, the middle part of the silica gel plug 10 is provided with a flow guide channel, the flow guide channel is sealed by an elastic rubber plug 11, and a first bottle mouth 7 of the double-mouth flask 6 is communicated with the silica gel plug 10 through a flow guide pipe 12. When the culture dish 5 cultivates bacteria, the elastic rubber plug 11 is inserted into the silica gel plug 10, the bacteria are cultured in the culture dish 5, after the culture is finished, the elastic rubber plug 11 is pulled up, and the liquid culture medium flows into the double-mouth flask 6 through the flow guide channel and the flow guide pipe 12, so that the next culture separation can be carried out.
The bottom of box 1 is equipped with attemperator 13, and attemperator 13 is by arranging temperature sensor and the electric heating wire in box 1, and temperature sensor is used for monitoring the temperature in box 1, crosses when hanging down when the temperature in box 1, and electric heating wire work can rise the temperature in box 1 to satisfy the temperature demand that the bacterial strain was cultivateed.
The ventilator is arranged in the temperature adjusting device 13, the ventilator is arranged in the ventilation pipeline, the air inlet of the ventilation pipeline is communicated with the outside atmosphere, the air outlet of the ventilation pipeline is communicated with the inside of the box body 1, and the ventilator is started to realize the air circulation in the box body 1, so that the cleanness of the environment in the box body 1 is ensured.
The box body 1 is square, and the number of the isolation boxes 2 in the box body 1 is four.
The lateral surface coating of box 1 has the heat insulation reflection stratum, and the heat insulation reflection stratum is evenly paintd by heat-reflecting material and is formed to improve the heat preservation effect of whole box 1.
The box body 1 is provided with a control panel, and the operation of the control panel is used for controlling the work of the electric heating wire so as to regulate and control the temperature in the box body 1.
When the culture box is used, the top cover at the top of the body 1 of the isolation box 2 is opened, the elastic rubber plug 11 is inserted into the silica gel plug 10, the culture dish 5 is isolated from the double-mouth flask 6 below, a culture medium is placed into the culture dish 5 for separation and purification, the culture dish 5 cultures a solid culture medium, the solid culture medium is changed into liquid in the culture dish 5, the elastic rubber plug 11 is pulled up, the liquid culture medium enters the double-mouth flask 6 through the liquid unloading port 9 and the draft tube 12, after the strains are propagated in the double-mouth flask 6 in a large quantity, the partition plate is opened, phage stock solution is added into the second bottle port 8 for culture, and the efficient culture of the strains is achieved.
The utility model discloses a be divided into a plurality of shielded cells 2 with box 1, the culture medium of solid cultivates in culture dish 5, form the culture solution after the cultivation is accomplished, with the mode that solid/liquid conversion combines, staphylococcus aureus once cultivates the finished product in culture dish 5 of independently cutting apart, guarantee that whole culture process operates under aseptic environment, staphylococcus aureus purification separation and transition to cultivation reproduction in culture dish 5, and is easy and simple to handle, avoid many times conversion culture dish and cause the pollution to the culture solution.
The foregoing is considered as illustrative and not restrictive of the preferred embodiments of the invention, and any modifications, equivalents and improvements made within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (4)
1. The phage culture device is characterized by comprising a box body (1), wherein a plurality of isolation boxes (2) are arranged in the box body (1), the isolation boxes (2) are divided into a first chamber (3) and a second chamber (4) through partition plates, the first chamber (3) is arranged above the second chamber (4), and the height of the first chamber (3) is smaller than that of the second chamber (4);
a culture dish (5) is arranged in the first chamber (3), and the culture dish (5) is arranged in the middle of the first chamber (3);
a double-mouth flask (6) is arranged in the second cavity (4), the bottom of the double-mouth flask (6) is placed on the bottom plate of the second cavity (4), a first bottleneck (7) and a second bottleneck (8) of the double-mouth flask (6) are arranged in parallel, and the first bottleneck (7) is placed below the culture dish (5);
the bottom of the culture dish (5) is provided with a liquid discharging port (9), the liquid discharging port (9) is sealed through a silica gel plug (10), the middle part of the silica gel plug (10) is provided with a flow guide channel, and the flow guide channel is sealed through an elastic rubber plug (11);
the first bottle mouth (7) of the double-mouth flask (6) is communicated with the silica gel plug (10) through the draft tube (12).
2. A phage display apparatus as claimed in claim 1, characterized in that the bottom of said box (1) is provided with a temperature-regulating device (13).
3. A phage culture apparatus according to claim 2, characterized in that the number of said isolation boxes (2) is four.
4. A phage culture apparatus according to claim 3, characterized in that the outer side of the housing (1) is coated with a heat-insulating reflective layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222934690.0U CN218465843U (en) | 2022-11-04 | 2022-11-04 | Phage culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222934690.0U CN218465843U (en) | 2022-11-04 | 2022-11-04 | Phage culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218465843U true CN218465843U (en) | 2023-02-10 |
Family
ID=85150157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222934690.0U Active CN218465843U (en) | 2022-11-04 | 2022-11-04 | Phage culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218465843U (en) |
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2022
- 2022-11-04 CN CN202222934690.0U patent/CN218465843U/en active Active
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