CN218412559U - Immunoblotting antibody recovery incubation box - Google Patents
Immunoblotting antibody recovery incubation box Download PDFInfo
- Publication number
- CN218412559U CN218412559U CN202221983685.2U CN202221983685U CN218412559U CN 218412559 U CN218412559 U CN 218412559U CN 202221983685 U CN202221983685 U CN 202221983685U CN 218412559 U CN218412559 U CN 218412559U
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- box body
- box
- sealing
- antibody recovery
- lid
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- 238000011534 incubation Methods 0.000 title claims abstract description 23
- 238000003119 immunoblot Methods 0.000 title claims abstract description 19
- 238000011084 recovery Methods 0.000 title claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 38
- 230000007246 mechanism Effects 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 9
- 230000009286 beneficial effect Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012296 anti-solvent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The utility model relates to an immunoblotting antibody recovery incubation box belongs to incubation box technical field. An immunoblot antibody recovery incubation cartridge comprising: the novel bottle cap comprises a box body, a box cover and a bottle cap, wherein the box body is in a trapezoid shape, a bottle opening is formed in one end of the box body, the bottle cap is arranged on the bottle opening, an opening is formed in the top of the box body, a sealing cover is arranged at the opening and used for sealing the opening, a sealing body is arranged on the surface of the end, close to the inside of the box body, a sealing gasket is arranged on the surface of the sealing body, a filter rod is arranged inside the box body and is arranged at the end, close to the bottle opening, of the filter rod, and a fastening mechanism is arranged between the box body and the box cover. Has the advantages that: through setting up the seal and sealing up the pad, can let box body and lid accomplish whole sealed effect, and be provided with the filter rod in inside, but filter solution can prevent a large amount of losses of anti solution through fastening device.
Description
Technical Field
The utility model belongs to the technical field of hatch box, concretely relates to immunoblotting antibody retrieves and hatches box.
Background
In molecular biology research, the Western Blot (Western Blot) is a commonly used experimental technique, i.e., a target protein is transferred to a PVDF membrane after electrophoretic separation, then is hybridized with a known protein antibody, and finally is qualitatively and quantitatively analyzed through a color reaction of an enzyme system. The method comprises five processes of electrophoresis, transfer, blocking, hybridization and color development, wherein the blocking process and the hybridization process can be jointly called as an antibody incubation process; when the existing shaking table is used for incubation, a plastic container sideslips and dumps, an antibody solution leaks and splashes out, and the like, so that an anti-solution is greatly lost, and the incubation is uneven or fails; at the same time, the anti-solution is difficult to recover after the incubation is finished. The Western Blot experiment has complicated steps, antigen-antibody combination is a key step, and the primary antibody is expensive, so the experiment cost waste caused by the problems is very large.
SUMMERY OF THE UTILITY MODEL
The utility model provides a solve above-mentioned technical problem and provide an immunoblotting antibody recovery incubation box, through setting up the seal and sealed the pad, can let box body and lid accomplish whole sealed effect, and be provided with the filter rod in inside, filterable solution can prevent a large amount of losses of antisolvent through fastening device.
The utility model provides an above-mentioned technical problem's technical scheme as follows: the immunoblot antibody recovery incubation box comprises: the novel bottle cap comprises a box body, a box cover and a bottle cap, wherein the box body is in a trapezoid shape, a bottle opening is formed in one end of the box body, the bottle cap is arranged on the bottle opening, an opening is formed in the top of the box body, a sealing cover is arranged at the opening and used for sealing the opening, a sealing body is arranged on the surface of the end, close to the inside of the box body, a sealing gasket is arranged on the surface of the sealing body, a filter rod is arranged inside the box body and is arranged at the end, close to the bottle opening, of the filter rod, and a fastening mechanism is arranged between the box body and the box cover.
Has the beneficial effects that: through setting up the seal and sealing up the pad, can let box body and lid accomplish whole sealed effect, and be provided with the filter rod in inside, but filter solution can prevent a large amount of losses of anti solution through fastening device.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
Further, the size and the shape of the sealing body are the same as those of the inner space of the box body, and the thickness of the sealing body is one fifth of that of the inner space of the box body.
The beneficial effect of adopting the above further scheme is: the sealing body is the same with the inner space of the box body, and the sealing gasket is arranged on the surface of the sealing body, so that the box body and the box cover can achieve the whole sealing effect.
Further, the bottle cap is connected with the bottle mouth through threads.
The beneficial effect of adopting the further scheme is that: the assembly and disassembly are convenient, and the sealing effect is good.
Further, the opening part is provided with a hinged seat, and the box cover is provided with a hinged end matched with the hinged seat.
The beneficial effect of adopting the further scheme is that: the box cover is convenient to open and close.
Further, fastening device includes buckle and buckle cap, the buckle has two, two the buckle is fixed respectively the both sides surface of box body, the buckle cap has two, two the buckle cap is fixed respectively the both sides surface of lid, the inner end surface of buckle cap be equipped with buckle complex fixture block.
The beneficial effect of adopting the further scheme is that: the fixing effect is good, and the solution leakage is prevented.
Furthermore, one end of the buckle cap connected with the box cover is made of elastic plastic materials.
The beneficial effect of adopting the further scheme is that: convenient joint, long service life.
Further, the length of the box body is 9cm, the width of the box body is 6cm, and the height of the box body is 3.2cm.
The beneficial effect of adopting the further scheme is that: the requirement of antibody incubation is met.
Drawings
FIG. 1 is a schematic diagram of the main structure of an immunoblotting antibody recovery incubation box of the present invention;
fig. 2 is a schematic diagram of a box body structure of the immunoblotting antibody recovery incubation box of the present invention;
fig. 3 is a schematic structural view of a box cover of the immunoblotting antibody recovery incubation box of the present invention;
FIG. 4 is an enlarged view of FIG. 2 at A;
FIG. 5 is an enlarged view of FIG. 3 at B;
FIG. 6 is an enlarged view at C of FIG. 2;
FIG. 7 is an enlarged view of FIG. 3 at D;
fig. 8 is a partial sectional view of the bottle cap connection of the immunoblotting antibody recovery incubation box of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a case body; 2. a box cover; 3. a seal body; 4. a bottle mouth; 5. buckling; 6. a hinged end; 7. a gasket; 8. a bottle cap; 9. a snap cap; 10. a hinged seat; 11. a clamping block; 12. a filter rod.
Detailed Description
The principles and features of the present invention will be described with reference to the drawings, which are provided for illustration only and are not intended to limit the scope of the invention.
Examples
As shown in fig. 1-8, this embodiment provides an immunoblotting antibody recovery incubation box, which includes a box body 1, a box cover 2 and a bottle cover 8, wherein the box body 1 is trapezoidal, a bottle mouth 4 is provided at the trapezoidal upper end of the box body 1, an external thread is provided at the periphery of the bottle mouth 4, an internal thread is provided on the inner surface of the bottle cover 8, the bottle mouth 4 and the bottle cover 8 are in threaded connection through respective threads, a hinge seat 10 is provided at one end of the bottle mouth 4 of the box body 1, the thickness and width of the hinge seat 10 are the same as those of the box body 1, the box cover 2 is the same as the box body 1 in shape, a hinge end 6 is provided at the trapezoidal upper end of the box cover 2, the box body 1 and the box cover 2 are in hinged connection with the hinge seat 10 through the hinge end 6, a sealing body 3 is provided on the surface of one end of the box cover 2, and the size and shape of the sealing body 3 are the same as the inner space of the box body 1, the thickness of the box is one fifth of the inner space of the box body 1, the surface of the sealing body 3 is provided with the sealing gaskets 7, the sealing gaskets 7 are arranged in two rows, the box cover 2 is connected with the box body 1 in a sealing mode through the sealing body 3 and the sealing gaskets 7, the sealing body 3 is the same as the inner space of the box body 1, the surface of the sealing body 3 is provided with the sealing gaskets 7, the box body 1 and the box cover 2 can achieve the whole-course sealing effect, the outer surfaces of the two sides of the box body 1 are respectively provided with the buckles 5, the outer surfaces of the two sides of the box cover 2 are respectively provided with the buckle caps 9, one end, connected with the box cover 2, of each buckle cap 9 is made of the existing elastic plastic material, the inner end surface of each buckle cap 9 is provided with the clamping block 11, and when the box cover 2 is connected with the box body 1 in a sealing mode, the clamping connection of the clamping block 11 and the buckles 5 can achieve the reinforcing and fixing effects on the sealing connection; lid 2 can let lid 2 open and shut more simply and easily with the buckle connection of the hinge structure of box body 1, conveniently takes or puts into test material, and 1 whole length of box body is 9cm, the width is 6cm, highly is 3.2cm, the inside filter rod 12 that is equipped with of box body, and filter rod 12 is established and is served near 1 trapezoidal one of box body.
The utility model discloses a theory of operation does: when using the device, box body 1 is connected with lid 2 and is done sealing connection through seal 3, because seal 3 is the same with the inner space of box body 1 and seal 3's surface is equipped with sealed pad 7, can let box body 1 accomplish whole sealed effect with lid 2, because articulated seat 10 thickness and width are the same with box body 1's thickness, sealing connection is accomplished equally with lid 2's articulated department to box body 1, when lid 2 and box body 1 sealing connection, accessible fixture block 11 is connected with buckle 5's buckle and accomplishes reinforcement and fixed action to sealing connection, bottleneck 4 can be dismantled at any time with the threaded connection of bottle lid 8.
In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (7)
1. The utility model provides an immunoblotting antibody recovery incubation box, its characterized in that, includes box body (1), lid (2) and bottle lid (8), box body (1) is trapezoidal form, the one end of box body (1) is equipped with bottleneck (4), be equipped with on bottleneck (4) bottle lid (8), the top of box body (1) is equipped with the opening, the opening part is equipped with and is used for the closing cap the open-ended lid (2), lid (2) are close to the surface of the inside one end of box body (1) is equipped with seal (3), seal (3) surface is equipped with sealed pad (7), the inside of box body (1) is equipped with filter rod (12), filter rod (12) set up and are being close to the one of bottleneck (4) is served, box body (1) with be equipped with fastening device between lid (2).
2. The immunoblot antibody recovery incubation cassette of claim 1, wherein the sealing body (3) has the same size and shape as the internal space of the cassette (1), and the thickness is one fifth of the internal space of the cassette (1).
3. Immunoblot antibody recovery incubation cassette according to claim 1, wherein the cap (8) is screwed to the mouth (4).
4. The immunoblot antibody recovery incubation box according to claim 1, wherein the opening is provided with a hinged seat (10), and the box cover (2) is provided with a hinged end (6) matched with the hinged seat (10).
5. The immunoblotting antibody recovery incubation box according to claim 1, wherein the fastening mechanism comprises two buckles (5) and two buckle caps (9), two buckles (5) are respectively fixed on the outer surfaces of the two sides of the box body (1), two buckle caps (9) are respectively fixed on the outer surfaces of the two sides of the box cover (2), and the inner end surface of the buckle cap (9) is provided with a clamping block (11) matched with the buckle (5).
6. The immunoblotting antibody recovery incubator box according to claim 5, wherein the end of the snap cap (9) connected to the box cover (2) is made of elastic plastic material.
7. The immunoblot antibody recovery incubation cassette according to any of claims 1-6, wherein the cassette body (1) has a length of 9cm, a width of 6cm and a height of 3.2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221983685.2U CN218412559U (en) | 2022-07-29 | 2022-07-29 | Immunoblotting antibody recovery incubation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221983685.2U CN218412559U (en) | 2022-07-29 | 2022-07-29 | Immunoblotting antibody recovery incubation box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218412559U true CN218412559U (en) | 2023-01-31 |
Family
ID=85013093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221983685.2U Active CN218412559U (en) | 2022-07-29 | 2022-07-29 | Immunoblotting antibody recovery incubation box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218412559U (en) |
-
2022
- 2022-07-29 CN CN202221983685.2U patent/CN218412559U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231012 Address after: 710000 169 Changle West Road, new town, Xi'an, Shaanxi Patentee after: THE FOURTH MILITARY MEDICAL University Address before: Room 401, Gate 2, 13th Floor, No. 28, Fuxing Road, Haidian District, Beijing, 100039 Patentee before: Lv Peiyuan |