CN115322868A - 96 ally oneself with PCR board double seal flat lids - Google Patents
96 ally oneself with PCR board double seal flat lids Download PDFInfo
- Publication number
- CN115322868A CN115322868A CN202211110315.2A CN202211110315A CN115322868A CN 115322868 A CN115322868 A CN 115322868A CN 202211110315 A CN202211110315 A CN 202211110315A CN 115322868 A CN115322868 A CN 115322868A
- Authority
- CN
- China
- Prior art keywords
- pcr
- plate
- sealing
- pcr plate
- double
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000007789 sealing Methods 0.000 claims abstract description 44
- 239000004033 plastic Substances 0.000 claims abstract description 19
- 238000009434 installation Methods 0.000 claims abstract description 18
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 10
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 10
- 241001330002 Bambuseae Species 0.000 claims description 10
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 10
- 239000011425 bamboo Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000003753 real-time PCR Methods 0.000 abstract description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 48
- 239000004743 Polypropylene Substances 0.000 description 15
- 229920001155 polypropylene Polymers 0.000 description 15
- 238000010586 diagram Methods 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002313 adhesive film Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Closures For Containers (AREA)
Abstract
The invention discloses a 96-linked PCR plate double-sealing flat cover, which is characterized by comprising a cover plate arranged at one end of a PCR plate, wherein the cover plate comprises a PP plastic plate and installation cylinders, the PP plastic plate is of a flat structure, 96 installation cylinders are uniformly arranged on the PP plastic plate, 96 installation cylinders are arranged corresponding to 96 holes, the main body of each installation cylinder is cylindrical, two convex structures are arranged on the cylinder, and the PCR plate is sealed through the convex structures. The structure adopts 96 linkage covers to seal the 96-hole PCR plate, and the 96-hole PCR plate can be disassembled, so that the problems of untight sealing of various sealing plate films in the high-low temperature environment, such as bulge, depression, liquid leakage, pollution and the like, in the fluorescent quantitative PCR process are solved.
Description
Technical Field
The invention belongs to the technical field of PCR plates, and particularly relates to a 96-connection cover capable of sealing a 96-hole PCR plate, a detachable 96-hole PCR plate and an eight-connection pipe, which can be used for sealing for multiple times.
Background
The PCR plate is a carrier mainly used as a primer participating in an amplification Reaction, taq DNA Polymerase, dNTP, template nucleic acid, mg, buffer solution and the like in Polymerase Chain Reaction (PCR).
The material of the PCR reaction kettle mainly takes polypropylene (PP) at present, can better adapt to repeated high and low temperature setting in the PCR reaction process, and can realize high-temperature high-pressure sterilization operation. For high throughput operation in conjunction with a line gun, PCR machine, etc., 96-well or 384-well PCR plates are more commonly used. The plate type meets the SBS international standard, and can be divided into four design modes of a skirt-free edge, a half skirt edge, a rising skirt edge and a full skirt edge according to skirt edge design in order to adapt to PCR instruments of different manufacturers.
Commercially available 0.2ml half skirt 96 well PCR plates, 0.2ml skirt-free 96 well PCR plates, 0.1ml skirt-free 96 well PCR plates, etc., are similar in structure, and are 96 wells, and a flat plate is arranged on the plate, as shown in FIG. 1.
The sealed 96-well PCR plate is now made by means of a plate sealing film, such as an adhesive film, a pressure sensitive film, a heat sealing film, etc. The disadvantages are as follows: under the environment of low temperature and high temperature, various conditions such as 'bulge', 'dent', 'untight seal' and the like can occur, thereby affecting the effect of the fluorescence PCR amplification, as shown in FIG. 2.
This is because: 1, the adhesive film has insufficient viscosity at low temperature, and the swelling and sealing are not tight when the adhesive is melted at high temperature. 2, the pressure-sensitive film has shrinkage deformation at low temperature and has poor sealing such as recess at high temperature. 3, while heat-sealable films perform well at low and high temperatures, the principle of heat-sealable films is that the films and 96-well PCR plates are melted together at high temperatures and are therefore difficult to tear apart after cooling.
Secondly, in the use process, the situation that sample adding is needed often exists, and the existing 96-well PCR plate can not realize sample adding because sealing can not be carried out after opening.
There is therefore a need to solve the above problems.
Disclosure of Invention
In order to solve the problem that various sealing membranes are not tightly sealed in the high-low temperature environment in the fluorescent quantitative PCR process, the primary object of the invention is to provide a 96-linked PCR plate double-sealing flat cover, the 96-linked cover is adopted to seal a 96-hole PCR plate, and the 96-hole PCR plate can be disassembled, so that the problems of the various sealing membranes, such as bulge, depression, liquid leakage, pollution and the like, which are not tightly sealed in the high-low temperature environment in the fluorescent quantitative PCR process are solved.
Another objective of the present invention is to provide a double-sealed flat lid for a 96-PCR plate, which can be opened for sample application and then tightly sealed for convenient use.
In order to achieve the above object, the technical solution of the present invention is as follows.
The utility model provides a 96 ally oneself with PCR board double seal flat lids, is including installing the apron in PCR board one end, the apron is including PP plastic slab and installation section of thick bamboo, the PP plastic slab is a smooth structure, and evenly is provided with 96 installation sections of thick bamboo on the PP plastic slab, and 96 installation sections of thick bamboo are corresponding to 96 hole settings, and the main part of every installation section of thick bamboo is cylindricly, be provided with two bellied structures on the cylinder, seal the PCR board through protruding structure.
Two bellied structures mainly function seals, can cooperate the PCR board to open, then the application of sample again, and the application of sample then the protruding seal of passing through again can realize the strict sealing to the PCR board to utilize the user to carry out the application of sample.
Further, sealed flat lid can also be including the PCR board, the PCR board is provided with the sealed tube, the sealed tube corresponds the setting with the hole, extends out from the PCR board to the cover is on the installation section of thick bamboo, and the arch through the installation section of thick bamboo cooperates with the sealed tube, realizes sealedly.
Further, the protrusions are one large and one small, that is, the two protrusions are not the same. Therefore, the two bulges are buckled into one bulge in a large size, the sample can be opened after transportation, and the sample can be buckled into the second bulge after the sample is added, so that the sample can be completely sealed.
Further, the protrusion is annular or oval.
Furthermore, a sealing cylinder is arranged on the bulge, and a sample is contained through the sealing cylinder.
Further, the sealing cylinder is wedge-shaped, so that the sample can be well accommodated.
The invention has the beneficial effects that:
the invention solves the sealing problem by arranging the convex structure on the PP plastic plate, solves the problem that the sealing plate film of the 96-hole PCR plate in the current market is not tightly sealed at low temperature and high temperature, does not generate the conditions of bulging, sinking, liquid leakage, poor sealing and laboratory pollution, and provides more stable and reliable effects for reagent storage, transportation and PCR amplification in a molecular laboratory.
Meanwhile, the sample can be added at any time through the convex structure, so that the use by a user is facilitated.
Drawings
FIG. 1 is a schematic representation of a 96-well PCR plate of the prior art.
FIG. 2 is a schematic diagram of a sealed 96-well PCR plate of the prior art.
Fig. 3 is a schematic structural diagram of a first implementation manner of the present invention.
Fig. 4 is a cross-sectional view of a first implementation of the invention.
Fig. 5 is a schematic structural diagram of a second implementation manner of the invention.
Fig. 6 is a side view of a second implementation of the invention.
FIG. 7 is a schematic diagram of a PCR plate according to a second embodiment of the present invention.
FIG. 8 is a sectional view of a PCR plate according to a second embodiment of the present invention.
Fig. 9 is a cross-sectional view of a second implementation of the invention.
Fig. 10 is a partially enlarged view of a portion a in fig. 9.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 3 and 4, the double-sealing flat cover for a 96-unit PCR plate, which is provided by the invention, comprises a cover plate installed at one end of the PCR plate, wherein the cover plate comprises a PP plastic plate 1 and installation cylinders 11, the PP plastic plate 1 is of a flat structure, 96 installation cylinders 11 are uniformly arranged on the PP plastic plate 1, 96 installation cylinders 11 are arranged corresponding to 96 holes of the 96-unit PCR plate, the main body of each installation cylinder 11 is cylindrical, two raised structures 111 and 112 are arranged on the cylinder, and the PCR plate is sealed through the raised structures 111 and 112.
The structure of two archs 111, 112 is mainly used to seal, can cooperate the PCR board to open, then the application of sample again, and the application of sample then seals through the arch again, and the rigorously sealed to the PCR board can be realized to the arch 111, 112 of bilayer to utilize the user to carry out the application of sample.
As shown in fig. 5 and 6, the sealing flat cover may further include a PCR plate 2, and in a normal case, the PCR plate 2 is provided with a sealing tube 3, the sealing tube 3 is disposed corresponding to 96 holes, extends from the PCR plate 2, and is sleeved on the mounting barrel 11, and the sealing is achieved by the protrusions 111 and 112 of the mounting barrel 11 cooperating with the sealing tube.
The PP plastic plate 1 and the PCR plate 2 are attached together.
The projections 111, 112 are large and small, and usually, the outermost projection 111 is slightly smaller to be fitted into the seal tube 3, and the inner projection 112 is slightly larger to achieve a good seal. Therefore, the two protrusions 111 and 112, one large one and the other small one, are firstly buckled into the first protrusion 111 (the outer protrusion 111 is buckled firstly to facilitate operation), the sample can be opened after transportation, and the sample can be buckled into the second protrusion 112 after the sample is added, so that the sample can be completely sealed.
The projections 111, 112 are generally annular or oval in shape.
Referring to fig. 7 and 8, in order to be able to cooperate with the protrusions 111 and 112, a sealing tube 3 is disposed on one side of the hole 21 of the PCR plate 2, the sealing tube 3 is used to cooperate with the mounting tube 11, the sealing tube 3 is illustrated as a wedge shape in the figure so as to contain a sample, the sealing tube 3 is sealed by the protrusions 111 and 112 on the mounting tube 11, as shown in fig. 9 and 10, the protrusions 111 and 112 can be snapped onto the inner wall of the sealing tube 3 to achieve sealing.
During manufacturing, the PP plastic is manufactured into a specific shape through manufacturing a mold and an injection molding technology to form the PP plastic plate 1, and usually, 96 bulges are connected to one PP plastic plate with the length of about 127 mm and the width of 87 mm and are arranged according to 8-by-12, so that the whole product is molded at one time without assembly.
Wherein, a mounting tube 11 is formed on one surface of the PP plastic plate 1 by injection molding, and the mounting tube 11 is provided with bulges 111 and 112 for sealing use.
The PCR plate 2 can be manufactured by the existing manufacturing method, and the sealing tube 3 on the PCR plate 2 is also manufactured and molded together.
In a word, the sealing problem is solved by arranging the convex structure on the PP plastic plate, the conditions that the sealing plate film of the 96-hole PCR plate in the current market is not tightly sealed at low temperature and high temperature are solved, and the conditions of bulging, sinking, liquid leakage, poor sealing and laboratory pollution are avoided, so that more stable and reliable effects are provided for reagent storage, transportation and PCR amplification in a molecular laboratory.
Meanwhile, the sample can be added at any time through the convex structure, so that the use by a user is facilitated.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The utility model provides a 96 ally oneself with PCR board double seal flat lids, its characterized in that is including installing the apron in PCR board one end, the apron is including PP plastic slab and installation section of thick bamboo, the PP plastic slab is a smooth structure, and evenly is provided with 96 installation sections of thick bamboo on the PP plastic slab, and 96 installation sections of thick bamboo are corresponding to 96 hole settings, and the main part of every installation section of thick bamboo is cylindric, be provided with two bellied structures on the cylinder, seal up the PCR board through protruding structure.
2. The double-sealing flat cover for a 96-up PCR plate as claimed in claim 1, wherein the sealing flat cover further comprises a PCR plate, the PCR plate is provided with a sealing tube, the sealing tube is arranged corresponding to the hole, extends from the PCR plate, is sleeved on the mounting barrel, and is sealed by the cooperation of the protrusion of the mounting barrel and the sealing tube.
3. The double-sealed flat lid for a 96-up PCR plate as claimed in claim 1, wherein the projections are one large and one small.
4. The double-sealed flat lid for a 96-up PCR plate as claimed in claim 1, wherein the protrusion has a ring shape or an oval shape.
5. The double-sealed flat cover for a 96-up PCR plate as claimed in claim 1, wherein the projections are provided with sealing cylinders through which the samples are loaded.
6. The double-sealed flat lid for a 96-up PCR plate as claimed in claim 2, wherein the sealing cylinder is wedge-shaped to well hold the sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211110315.2A CN115322868A (en) | 2022-09-13 | 2022-09-13 | 96 ally oneself with PCR board double seal flat lids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211110315.2A CN115322868A (en) | 2022-09-13 | 2022-09-13 | 96 ally oneself with PCR board double seal flat lids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115322868A true CN115322868A (en) | 2022-11-11 |
Family
ID=83930171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211110315.2A Pending CN115322868A (en) | 2022-09-13 | 2022-09-13 | 96 ally oneself with PCR board double seal flat lids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115322868A (en) |
-
2022
- 2022-09-13 CN CN202211110315.2A patent/CN115322868A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20070128080A1 (en) | Lidded vessel | |
| WO2006133617A1 (en) | Cover plate assembly for lithium ion battery, battery case and battery using the same | |
| CN206098472U (en) | Button battery | |
| CN115322868A (en) | 96 ally oneself with PCR board double seal flat lids | |
| CN110406810A (en) | Object storage component and aerosol device | |
| CN201179996Y (en) | Water bucket for drinking machines | |
| CN201519582U (en) | Centrifuge tube used in laboratory | |
| WO2023160091A1 (en) | Purification column | |
| CN216550413U (en) | Tube assembly for one-tube reagent freeze-drying and PCR reaction | |
| CN109738237A (en) | Fluid collection device | |
| CN202609280U (en) | Container cover | |
| CN212464680U (en) | Sealing cover and container device | |
| CN104845888A (en) | Totally-enclosed cell culture plate | |
| JP2014061918A (en) | Mouth sealing mechanism | |
| CN103221134A (en) | Closure assembly for cell culture apparatus | |
| CN206782421U (en) | The closure of hydraulic seal bucket | |
| CN207208889U (en) | Seal bucket and ventilative sealing bucket | |
| CN220126257U (en) | Novel reagent tube silica gel sealing plug | |
| CN218412559U (en) | Immunoblotting antibody recovery incubation box | |
| CN109730727A (en) | Subregion collector | |
| CN114874886A (en) | Reagent tube | |
| CN211197178U (en) | Novel sealing inner cover of plastic barrel | |
| CN218755737U (en) | Explosion-proof PCR pipe | |
| CN220263196U (en) | Auxiliary tool | |
| CN115353957A (en) | Biochemical reagent reaction assembly |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 523000 No. 8, South 6th Street, Heshi Road, Qiaotou Town, Dongguan, Guangdong Applicant after: Jihe life science and Technology (Dongguan) Co.,Ltd. Address before: 523000 room 108, building 2, No. 93, Dazhou Daxin Road, Qiaotou town, Dongguan City, Guangdong Province Applicant before: Jihe life science and Technology (Dongguan) Co.,Ltd. |