CN216485051U - Novel antibody incubation bag capable of exhausting gas automatically - Google Patents
Novel antibody incubation bag capable of exhausting gas automatically Download PDFInfo
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- CN216485051U CN216485051U CN202123154962.7U CN202123154962U CN216485051U CN 216485051 U CN216485051 U CN 216485051U CN 202123154962 U CN202123154962 U CN 202123154962U CN 216485051 U CN216485051 U CN 216485051U
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- bag
- negative pressure
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- head
- incubation bag
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- 238000011534 incubation Methods 0.000 title claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims description 5
- 210000003437 trachea Anatomy 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 3
- 238000013022 venting Methods 0.000 claims 2
- 239000003814 drug Substances 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000002026 carminative effect Effects 0.000 description 2
- 230000008034 disappearance Effects 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
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Abstract
The utility model discloses a novel antibody incubation bag capable of self-exhausting, which comprises an incubation bag, wherein the top end of the incubation bag forms a sealing structure through a bag head structure, one side of the bag head structure is communicated with a negative pressure device, the bag head structure comprises a fixed head, a middle frame and a sealing cover, the top end of the sealing cover is fixedly connected with the middle frame through screw threads, the fixed head is communicated with the incubation bag, the middle frame and the fixed head are clamped and fixed, the negative pressure device comprises a negative pressure ball, a gas pipe and a negative pressure switch, a user can rotate the sealing cover structure, then inject liquid medicine into the bag and rotate to one side through the middle frame structure at the top end, a rotating plate in the middle part can drive a rotating block at the middle part to rotate, the user can inject liquid medicine or open the negative pressure switch at one side, the negative pressure ball sucks gas in the incubation bag into the negative pressure ball, discharges redundant gas in the incubation bag, and then can close the negative pressure switch, the sealing cover structure is screwed, so that the internal air pressure is sealed, the leakage is avoided, and the internal sterile environment is ensured.
Description
Technical Field
The utility model relates to an incubation bag structure field especially relates to a novel antibody incubation bag that can self-exhaust.
Background
Western Blot, Western Blot (immunoblot assay), is a commonly used experimental method and detection technique in molecular biology, biochemistry and immunogenetics, and has been widely used in many fields such as gene expression research at protein level, antibody activity detection and early disease diagnosis. The basic principle is to stain a gel electrophoresis treated cell or biological tissue sample with a specific antibody. Information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the location and depth of staining. The specific operation method is that the total protein of the cell or tissue after electrophoretic separation is transferred to a solid support NC membrane or a PVDF membrane from gel, then a protein with a specific antigen is detected by a specific antibody, the specific antibody is generally divided into a primary antibody and a secondary antibody, the primary antibody is used for directly identifying and specifically binding the protein to be detected, and the secondary antibody is used for identifying and specifically binding the primary antibody and has the function of chemiluminescence, so that the detection is convenient. Primary antibody incubation is a very critical step throughout the WesternBlot process.
In chinese patent CN201820275547.6, a novel antibody incubation bag is introduced, wherein the present invention can exhaust the gas in the incubation bag, so that the membrane for transferring target protein is completely immersed in the antibody solution for sufficient incubation, thereby increasing the success rate of the experiment, however, in the actual operation, the person skilled in the art finds that: this structure has good experiment success rate, but all needs external equipment to carry out the exhaust operation at every turn, and the result of use in this respect is not good, and the practicality is relatively poor.
SUMMERY OF THE UTILITY MODEL
In order to overcome the relatively poor not enough of prior art's practicality, the utility model provides a can incubate bag from carminative novel antibody, after the user can unscrew the closing cap structure, pour into the liquid medicine into the bag, and the center structure through the top is to one side rotating, and the rotor plate wherein can drive the turning block at middle part and rotate, the user can adjust through the position relation between the fixed plate with the bottom, when the disappearance position overlaps, the user can pour into the liquid medicine into, or open negative pressure switch in one side, the gas in the negative pressure ball will be incubated the bag inhales to the negative pressure ball in, discharge the unnecessary gas in the bag of will incubating, afterwards can close negative pressure switch, the closing cap structure of screwing, and then seal inside atmospheric pressure, avoid leaking, and guarantee inside sterile environment.
In order to solve the technical problem, the utility model provides a following technical scheme: the utility model provides a can breed bag from carminative novel antibody, is including hatching and breeds the bag, the top of hatching and breeding the bag forms seal structure through the bag head structure, one side intercommunication of bag head structure has negative pressure device, including fixed head, center and closing cap in the bag head structure, the top and the center of closing cap pass through screw thread fixed connection, the fixed head with hatch the bag intercommunication, the center is established fixedly with the fixed head card, including negative pressure ball, trachea and negative pressure switch in the negative pressure device.
As a preferred technical scheme of the utility model, fixed head and center are the hollow frame structure of upper and lower no face, the top outside of fixed head all is provided with protruding structure with the bottom inboard of center, the middle part of fixed head is provided with the dead lever, the outside of dead lever is passed through the auxiliary rod and is realized fixing with the inside wall of fixed head.
As a preferred technical scheme of the utility model, the top activity of dead lever is provided with changes the piece, the outside of changeing the piece is fixed with the rotor plate, and the outside of dead lever is fixed with the fixed plate, just be provided with sealed glue structure between rotor plate and the fixed plate, just sealed glue structure bonds and is fixed in the top surface of fixed plate.
As a preferred technical scheme of the utility model, fixed plate and rotor plate are the circular platelike structure who has the breach, just the shape looks adaptation of fixed plate and rotor plate forms complementary structure in the shape.
As a preferred technical scheme of the utility model, the helicitic texture has all been seted up in the outside of closing cap and center, just the gas pocket structure has been seted up to one side of center, trachea and gas pocket structure intercommunication, be provided with negative pressure switch on the trachea, tracheal other end and negative pressure ball intercommunication.
As an optimal technical scheme of the utility model, the top of dead lever is provided with protruding structure, its the bottom of changeing the piece is seted up rather than the hole structure that changes of size looks adaptation.
As an optimized technical proposal of the utility model, the inner side of the sealing cover is provided with a sealing gasket.
Compared with the prior art, the utility model discloses the beneficial effect that can reach is:
in this device, through the bag head structure that sets up, after the user can unscrew the closing cap structure, pour into the liquid medicine in the bag, and the center structure through the top is to one side rotation, and rotor plate wherein can drive the commentaries on classics piece at middle part and rotate, the user can adjust through the position relation between the fixed plate with the bottom, when the disappearance position overlaps, the user can pour into the liquid medicine, or open negative pressure switch in one side, negative pressure ball will hatch the gas in the bag and inhale to negative pressure ball in, will hatch unnecessary gas outgoing in the bag, can close negative pressure switch afterwards, the closing cap structure of screwing, and then seal the atmospheric pressure of inside, avoid leaking, and guarantee inside sterile environment.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the present invention when removing the cap;
fig. 3 is a schematic structural view of the fixing head of the present invention;
fig. 4 is a schematic structural view of the fixing head and the fixing plate of the present invention;
fig. 5 is a schematic sectional view of the bag head structure of the present invention.
Wherein: 1. incubating bags; 2. a bag head structure; 21. a fixed head; 22. a middle frame; 23. sealing the cover; 24. fixing the rod; 241. an auxiliary lever; 242. rotating the block; 25. a fixing plate; 26. a rotating plate; 3. a negative pressure device; 31. a negative pressure ball; 32. an air tube; 33. and a negative pressure switch.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the functions of the invention easy to understand, the invention is further explained below with reference to the specific embodiments, but the following embodiments are only the preferred embodiments of the invention, not all. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative work belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example (b):
as shown in fig. 1-5, the utility model provides a novel antibody incubation bag capable of self-exhausting, including incubating bag 1, the top of incubating bag 1 forms the enclosed construction through bag head structure 2, and one side intercommunication of bag head structure 2 has negative pressure device 3, includes fixed head 21, center 22 and closing cap 23 in the bag head structure 2, and the top of closing cap 23 passes through screw thread fixed connection with center 22, and fixed head 21 communicates with incubating bag 1, and center 22 and fixed head 21 card are established fixedly, includes negative pressure ball 31, trachea 32 and negative pressure switch 33 in the negative pressure device 3.
In other embodiments, the fixing head 21 and the middle frame 22 are both hollow frame structures without upper and lower surfaces, the top outer side of the fixing head 21 and the bottom inner side of the middle frame 22 are both provided with a protruding structure, the middle part of the fixing head 21 is provided with a fixing rod 24, and the outer side of the fixing rod 24 is fixed to the inner side wall of the fixing head 21 through the auxiliary rod 241.
After the user can unscrew the structure of the sealing cover 23, the liquid medicine is injected into the bag and rotates to one side through the structure of the top middle frame 22, the rotating plate 26 therein can drive the rotating block 242 at the middle part to rotate, the user can adjust the position relationship with the fixing plate 25 at the bottom end, when the missing part is overlapped, the user can inject the liquid medicine, or the negative pressure switch 3 is opened at one side, the negative pressure ball 31 sucks the gas in the incubation bag 1 into the negative pressure ball 31, (when the user needs to ensure that the sealing cover 23 at the top end is tightly fixed with the middle frame 22 during the self-exhaust operation) exhausts the redundant gas in the incubation bag 1, then the negative pressure switch 33 can be closed, the structure of the sealing cover 23 is screwed, the internal air pressure is sealed, the external leakage is avoided, and the internal sterile environment is ensured.
In other embodiments, a rotating block 242 is movably disposed at the top end of the fixing rod 24, a rotating plate 26 is fixed at the outer side of the rotating block 242, a fixing plate 25 is fixed at the outer side of the fixing rod 24, and a sealant structure is disposed between the rotating plate 26 and the fixing plate 25 and is adhesively fixed on the top surface of the fixing plate 25.
The rotating block 242 drives the rotating plate 26 to rotate, so as to adjust the use function of the incubation bag, and the negative pressure device 3 at one side can be matched to realize the effect of exhausting the air inside.
In other embodiments, the fixed plate 25 and the rotating plate 26 are both circular plate-shaped structures with notches, and the shapes of the fixed plate 25 and the rotating plate 26 are matched to form structures complementary in shape.
Fixed plate 25 and rotor plate 26 can rotate under user's control, and the overlapping between fixed plate 25 and the rotor plate 26 with stagger the effect that realizes opening and closing of bag head structure 2, and establish fixedly through the card of bottom, the person of facilitating the use rotates and seals.
In other embodiments, the outer sides of the sealing cover 23 and the middle frame 22 are both provided with a threaded structure, one side of the middle frame 22 is provided with an air hole structure, the air pipe 32 is communicated with the air hole structure, the air pipe 32 is provided with a negative pressure switch 33, and the other end of the air pipe 32 is communicated with the negative pressure ball 31.
The negative pressure device 3 can actively discharge the redundant gas in the incubation bag to the outside, so as to meet the use requirement, and the negative pressure ball 31 has simple structure, convenient use and low manufacturing cost, and is suitable for large-scale use.
In other embodiments, the top end of the fixing rod 24 is provided with a protrusion structure, and the bottom end of the rotating block 242 is provided with a rotating hole structure matched with the size of the rotating block.
The hole rotating structure and the protrusions are matched with each other in the rotating process, so that the situation that the fixed rod 24 and the rotating block 242 are inclined is avoided.
In other embodiments, the inside of the cover 23 is provided with a sealing gasket.
The sealing washer structure can ensure the sealing performance when the middle frame 22 is fixed, and prevent external air from entering the inside to cause pollution.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A novel antibody incubation bag capable of self-exhausting comprises an incubation bag (1), and is characterized in that: incubate the top of bag (1) and form sealing structure through bag head structure (2), one side intercommunication of bag head structure (2) has negative pressure equipment (3), including fixed head (21), center (22) and closing cap (23) in bag head structure (2), the top and center (22) of closing cap (23) pass through screw thread fixed connection, fixed head (21) and incubation bag (1) intercommunication, center (22) are established fixedly with fixed head (21) card, including negative pressure ball (31), trachea (32) and negative pressure switch (33) in negative pressure equipment (3).
2. The novel self-ventilatable antibody incubation bag of claim 1, wherein: fixed head (21) and center (22) are the hollow frame structure of upper and lower no face, the top outside of fixed head (21) and the bottom inboard of center (22) all are provided with protruding structure, the middle part of fixed head (21) is provided with dead lever (24), the outside of dead lever (24) is passed through auxiliary rod (241) and is realized fixing with the inside wall of fixed head (21).
3. The novel self-ventilatable antibody incubation bag of claim 2, wherein: the top activity of dead lever (24) is provided with changes piece (242), the outside of changeing piece (242) is fixed with rotor plate (26), and the outside of dead lever (24) is fixed with fixed plate (25), just be provided with sealed glued structure between rotor plate (26) and fixed plate (25), just sealed glued structure bonding is fixed in the top surface of fixed plate (25).
4. The novel antibody incubation bag capable of self-venting as claimed in claim 3, wherein: the fixing plate (25) and the rotating plate (26) are both circular plate-shaped structures with notches, and the shapes of the fixing plate (25) and the rotating plate (26) are matched to form a complementary structure in shape.
5. The novel self-ventilatable antibody incubation bag of claim 1, wherein: the outer sides of the sealing cover (23) and the middle frame (22) are both provided with threaded structures, an air hole structure is formed in one side of the middle frame (22), the air pipe (32) is communicated with the air hole structure, a negative pressure switch (33) is arranged on the air pipe (32), and the other end of the air pipe (32) is communicated with a negative pressure ball (31).
6. The novel antibody incubation bag capable of self-venting as claimed in claim 3, wherein: the top end of the fixed rod (24) is provided with a protruding structure, and the bottom end of the rotating block (242) is provided with a rotating hole structure matched with the size of the rotating block.
7. The novel self-ventilatable antibody incubation bag of claim 1, wherein: and a sealing gasket is arranged on the inner side of the sealing cover (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123154962.7U CN216485051U (en) | 2021-12-15 | 2021-12-15 | Novel antibody incubation bag capable of exhausting gas automatically |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123154962.7U CN216485051U (en) | 2021-12-15 | 2021-12-15 | Novel antibody incubation bag capable of exhausting gas automatically |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216485051U true CN216485051U (en) | 2022-05-10 |
Family
ID=81423587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123154962.7U Expired - Fee Related CN216485051U (en) | 2021-12-15 | 2021-12-15 | Novel antibody incubation bag capable of exhausting gas automatically |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216485051U (en) |
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2021
- 2021-12-15 CN CN202123154962.7U patent/CN216485051U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220510 |