CN214325881U

CN214325881U - Anti-leakage nucleic acid extraction kit adopting magnetic bead method

Info

Publication number: CN214325881U
Application number: CN202023292253.0U
Authority: CN
Inventors: 张国宁; 王海岩; 李硕
Original assignee: Shandong Acv Biotech Co ltd
Current assignee: Shandong Acv Biotech Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-10-01
Anticipated expiration: 2030-12-30

Abstract

The utility model discloses a prevent magnetic bead method nucleic acid extraction kit of leakage, including lid, locating piece and extrusion spring, the fixed surface of lid is connected with the baffle, the bottom fixedly connected with fixture block of baffle, the surface connection of fixture block has the spacing ring, the both ends fixedly connected with box body of spacing ring, one side of box body is connected with sealed the pad, the surperficial gomphosis of sealed pad has the lid, the bottom fixedly connected with box body of locating piece, the top fixedly connected with pivot of locating piece, the surface through connection of pivot has the block. This prevent magnetic bead method nucleic acid extraction kit of leakage docks lid and box body to sealed the junction of lid and box body through the interference fit between sealed pad and the lid, the fixture block is fixed the lid through the block connection structure between spacing ring and the box body simultaneously, thereby makes the device reach the function that prevents to leak, leads to the pollution in order to prevent that reagent from leaking.

Description

Anti-leakage nucleic acid extraction kit adopting magnetic bead method

Technical Field

The utility model relates to a kit equipment technical field specifically is a prevent magnetic bead method nucleic acid extraction kit of leakage.

Background

Magnetic nucleic acid is a biological macromolecular compound polymerized from many nucleotide monomers, and is one of the most basic substances of life. Nucleic acid is the most important substance in all biological molecules and is widely present in all animal and plant cells and microorganisms. The extraction of nucleic acid with good integrity and high purity is of great significance to downstream molecular biology experiments. At present, the methods for extracting nucleic acid clinically mainly comprise a centrifugal column method, a magnetic bead method and the like, and the magnetic bead method has the advantages of good integrity and high purity of the obtained nucleic acid and has ideal application significance for downstream molecular biology experiments.

The magnetic bead method nucleic acid extraction kit is a box for storing nucleic acid extraction reagents, and is used for extracting pure nucleic acid from samples such as plasma, serum, ascites, tissue grinding supernatant, culture cell supernatant, exfoliated cell suspension, swabs and the like, and further used for subsequent molecular diagnosis. The device can be widely applied to genome research, molecular evolution research and research of genetic diseases in medicine in molecular biology, when the device is used, a container with a reagent is placed inside a box, the reagent can be stored, along with the development of science and technology, the magnetic bead method nucleic acid extraction kit has great development, the development of the device brings great convenience to people when storing nucleic acid extracting solution, and the type and the number of the device are increasing day by day.

Most of the nucleic acid extraction kits in the current market based on the magnetic bead method are simple paper packaging boxes, and the problems of reagent leakage and the like easily occur in the transportation process and the use process, so that pollution is caused, and the nucleic acid extraction kits in the current magnetic bead method are improved.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a prevent magnetic bead method nucleic acid extraction kit of leakage to the magnetic bead method nucleic acid extraction kit that the solution above-mentioned background art provided is mostly simple and easy paper packing carton, appears reagent leakage scheduling problem easily in the transportation and when using, thereby causes the problem of pollution.

In order to achieve the above object, the utility model provides a following technical scheme: a leakage-proof nucleic acid extraction kit adopting a paramagnetic particle method comprises a cover, a positioning block and an extrusion spring, wherein the surface of the cover is fixedly connected with a baffle, the bottom of the baffle is fixedly connected with a clamping block, the surface of the clamping block is connected with a limiting ring, two ends of the limiting ring are fixedly connected with a box body, one side of the box body is connected with a sealing gasket, and the surface of the sealing gasket is embedded with the cover;

the bottom of the positioning block is fixedly connected with a box body, the top of the positioning block is fixedly connected with a rotating shaft, the surface of the rotating shaft is connected with a clamping block in a penetrating mode, the surface of the clamping block is embedded in a clamping groove, the clamping groove is formed in the surface of the mounting frame, and the surface of the mounting frame is provided with the positioning block;

the one end fixedly connected with extrusion piece of extrusion spring, one side fixedly connected with connecting block of extrusion piece, the fixed surface of connecting block is connected with the mounting bracket, the one end of extrusion piece is provided with the mounting hole, the inside at the mounting bracket is seted up to the mounting hole.

Preferably, the fixture block is of a diamond structure, the top of the box body is provided with a recessed groove, the fixture block is tangent to the surface of the limiting ring, and the fixture block forms a clamping connection structure with the box body through the limiting ring.

Preferably, the cross section of the sealing gasket is of a semicircular structure, the sealing gasket is made of rubber, and the sealing gasket and the cover are in an interference fit connection structure.

Preferably, the clamping block forms a rotating connection structure with the positioning block through the rotating shaft, and the clamping block forms a riveting connection structure with the mounting frame through the clamping groove.

Preferably, the outer wall size of mounting bracket and the inner wall size phase-match of locating piece, and the mounting hole is equidistant distribution in the inside of mounting bracket, and the junction mutually perpendicular of locating piece and box body.

Preferably, the top of the extrusion block is of a semicircular structure, a sliding connection structure is arranged between the surface of the connecting block and the inner wall of the mounting frame, and the extrusion block forms an elastic telescopic connection structure with the mounting frame through the extrusion spring.

Compared with the prior art, the beneficial effects of the utility model are that:

1. this prevent magnetic bead method nucleic acid extraction kit of leakage docks lid and box body to sealed the junction of lid and box body through the interference fit between sealed pad and the lid, the fixture block is fixed the lid through the block connection structure between spacing ring and the box body simultaneously, thereby makes the device reach the function that prevents to leak, leads to the pollution in order to prevent that reagent from leaking.

2. This prevent magnetic bead method nucleic acid extraction kit of leakage docks mounting bracket and locating piece for the mounting bracket is placed inside the locating piece, and the block piece is rotated to the rethread pivot, makes block piece and block groove coincide each other, makes mutual riveting between block piece and the mounting bracket, thereby can be more convenient fix the mounting bracket.

3. This prevent magnetic bead method nucleic acid extraction kit of leakage, when the device uses, dock reagent pipe and mounting hole to make reagent pipe drive extrusion piece and remove to the one end of extrusion spring, until reagent pipe and mounting bracket coincide back each other, thereby can be more convenient extrude reagent pipe fixedly, avoid the device reagent pipe to rock when using.

Drawings

FIG. 1 is a schematic front view of the cross-section of the present invention;

FIG. 2 is an enlarged schematic view of the present invention;

fig. 3 is an enlarged schematic view of the present invention B.

In the figure: 1. a cover; 2. a baffle plate; 3. a clamping block; 4. a limiting ring; 5. a box body; 6. a gasket; 7. positioning blocks; 8. a rotating shaft; 9. a clamping block; 10. a clamping groove; 11. a mounting frame; 12. a compression spring; 13. extruding the block; 14. connecting blocks; 15. and (7) installing holes.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a leakage-proof nucleic acid extraction kit adopting a magnetic bead method comprises a cover 1, a baffle plate 2, a clamping block 3, a limiting ring 4, a box body 5, a sealing gasket 6, a positioning block 7, a rotating shaft 8, a clamping block 9, a clamping groove 10, a mounting frame 11, an extrusion spring 12, an extrusion block 13, a connecting block 14 and a mounting hole 15, wherein the surface of the cover 1 is fixedly connected with the baffle plate 2, the bottom of the baffle plate 2 is fixedly connected with the clamping block 3, the surface of the clamping block 3 is connected with the limiting ring 4, the clamping block 3 is of a diamond structure, the top of the box body 5 is provided with an inwards-recessed groove, the clamping block 3 is tangent to the surface of the limiting ring 4, the clamping block 3 forms a clamping connection structure with the box body 5 through the limiting ring 4, the cover 1 is conveniently fixed on the box body 5 through the clamping block 3, the two ends of the limiting ring 4 are fixedly connected with the box body 5, one side of the box body 5 is connected with the sealing gasket 6, the cover 1 is embedded on the surface of the sealing gasket 6, the section of the sealing gasket 6 is of a semicircular structure, the sealing gasket 6 is made of rubber, and an interference fit connection structure is formed between the sealing gasket 6 and the cover 1, so that the connection part of the cover 1 and the box body 5 can be conveniently sealed through the sealing gasket 6;

the bottom of the positioning block 7 is fixedly connected with a box body 5, the top of the positioning block 7 is fixedly connected with a rotating shaft 8, the surface of the rotating shaft 8 is connected with an engaging block 9 in a penetrating way, the surface of the engaging block 9 is embedded in the engaging groove 10, the engaging block 9 forms a rotating connection structure through the rotating shaft 8 and the positioning block 7, and the clamping block 9 forms a riveting connection structure with the mounting frame 11 through the clamping groove 10, so that the mounting frame 11 is fixed through the clamping block 9, the clamping groove 10 is arranged on the surface of the mounting frame 11, the positioning block 7 is arranged on the surface of the mounting frame 11, the size of the outer wall of the mounting frame 11 is matched with the size of the inner wall of the positioning block 7, the mounting holes 15 are distributed in the mounting rack 11 at equal intervals, and the connecting parts of the positioning blocks 7 and the box body 5 are perpendicular to each other, so that the mounting rack 11 and the positioning blocks 7 can be conveniently butted;

the one end fixedly connected with extrusion piece 13 of extrusion spring 12, one side fixedly connected with connecting block 14 of extrusion piece 13, the top of extrusion piece 13 is semi-circular structure, and is the sliding connection structure between the surface of connecting block 14 and the inner wall of mounting bracket 11 to extrusion piece 13 is through constituting the flexible connection structure of elasticity between extrusion spring 12 and the mounting bracket 11, is convenient for lead to extrusion piece 13 and prevents that the reagent pipe from rocking, the fixed surface of connecting block 14 is connected with mounting bracket 11, the one end of extrusion piece 13 is provided with mounting hole 15, mounting hole 15 sets up in the inside of mounting bracket 11.

The working principle is as follows: in the anti-leakage nucleic acid extraction kit adopting the magnetic bead method, firstly, the cover 1 is butted with the box body 5, the joint of the cover 1 and the box body 5 is sealed through the interference fit between the sealing gasket 6 and the cover 1, meanwhile, the cover 1 is fixed through the clamping connection structure between the limiting ring 4 and the box body 5 by the fixture block 3, so that the device achieves the function of preventing leakage, so as to prevent pollution caused by reagent leakage, the mounting frame 11 is butted with the positioning block 7, the mounting frame 11 is placed in the positioning block 7, then the clamping block 9 is rotated through the rotating shaft 8, so that the clamping block 9 is mutually matched with the clamping groove 10, the clamping block 9 is mutually riveted with the mounting frame 11, so that the mounting frame 11 can be more conveniently fixed, when the device is used, the reagent tube is butted with the mounting hole 15, and the reagent tube drives the extrusion block 13 to move towards one end of the extrusion spring 12, until the reagent tube is matched with the mounting frame 11, the reagent tube can be more conveniently extruded and fixed, and the reagent tube is prevented from shaking when the device is used, which is the characteristic of the anti-leakage nucleic acid extraction kit by the magnetic bead method.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A prevent magnetic bead method nucleic acid extraction kit of leakage, includes lid (1), locating piece (7) and extrusion spring (12), its characterized in that: the surface of the cover (1) is fixedly connected with a baffle (2), the bottom of the baffle (2) is fixedly connected with a fixture block (3), the surface of the fixture block (3) is connected with a limiting ring (4), two ends of the limiting ring (4) are fixedly connected with a box body (5), one side of the box body (5) is connected with a sealing gasket (6), and the surface of the sealing gasket (6) is embedded with the cover (1);

the bottom of the positioning block (7) is fixedly connected with a box body (5), the top of the positioning block (7) is fixedly connected with a rotating shaft (8), the surface of the rotating shaft (8) is connected with a clamping block (9) in a penetrating mode, the surface of the clamping block (9) is embedded in a clamping groove (10), the clamping groove (10) is formed in the surface of the mounting frame (11), and the surface of the mounting frame (11) is provided with the positioning block (7);

the one end fixedly connected with extrusion piece (13) of extrusion spring (12), one side fixedly connected with connecting block (14) of extrusion piece (13), the fixed surface of connecting block (14) is connected with mounting bracket (11), the one end of extrusion piece (13) is provided with mounting hole (15), the inside at mounting bracket (11) is seted up in mounting bracket (15).

2. A leak-proof magnetic bead method nucleic acid extraction kit as recited in claim 1, wherein: the fixture block (3) is of a diamond structure, the top of the box body (5) is provided with a recessed groove, the fixture block (3) is tangent to the surface of the limiting ring (4), and the fixture block (3) forms a clamping connection structure with the box body (5) through the limiting ring (4).

3. A leak-proof magnetic bead method nucleic acid extraction kit as recited in claim 1, wherein: the cross section of the sealing gasket (6) is of a semicircular structure, the sealing gasket (6) is made of rubber, and the sealing gasket (6) and the cover (1) are in an interference fit connection structure.

4. A leak-proof magnetic bead method nucleic acid extraction kit as recited in claim 1, wherein: the clamping block (9) and the positioning block (7) form a rotary connection structure through the rotating shaft (8), and the clamping block (9) and the mounting frame (11) form a riveting connection structure through the clamping groove (10).

5. A leak-proof magnetic bead method nucleic acid extraction kit as recited in claim 1, wherein: the outer wall size of mounting bracket (11) and the inner wall size phase-match of locating piece (7), and mounting hole (15) are equidistant distribution in the inside of mounting bracket (11), and the junction mutually perpendicular of locating piece (7) and box body (5).

6. A leak-proof magnetic bead method nucleic acid extraction kit as recited in claim 1, wherein: the top of the extrusion block (13) is of a semicircular structure, a sliding connection structure is arranged between the surface of the connecting block (14) and the inner wall of the mounting frame (11), and the extrusion block (13) forms an elastic telescopic connection structure with the mounting frame (11) through the extrusion spring (12).