CN208979498U - A kind of DNA methylation detection kit - Google Patents
A kind of DNA methylation detection kit Download PDFInfo
- Publication number
- CN208979498U CN208979498U CN201821612621.5U CN201821612621U CN208979498U CN 208979498 U CN208979498 U CN 208979498U CN 201821612621 U CN201821612621 U CN 201821612621U CN 208979498 U CN208979498 U CN 208979498U
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- Prior art keywords
- air bag
- box cover
- box body
- movable plate
- dna methylation
- Prior art date
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- 230000007067 DNA methylation Effects 0.000 title claims abstract description 25
- 238000001514 detection method Methods 0.000 title claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 37
- 230000013011 mating Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 9
- 239000000523 sample Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 229910021389 graphene Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000010309 melting process Methods 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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- 238000013139 quantization Methods 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model provides a kind of DNA methylation detection kit, it is related to molecular biology field, including reagent box body, air bag is equipped in reagent box body, air bag is set in spongy layer, spongy layer is cylindrical shape, the inner wall of spongy layer and the outer wall of air bag form ice bag storehouse, spongy layer is set in reagent box body, groove is equipped in reagent box body, the first screw thread is equipped in groove, reagent box body is mating to be equipped with box cover, the lower section respective slot of box cover is equipped with convex block, convex block is equipped with the second screw thread, convex block is connected through a screw thread with groove, movable plate is equipped in box cover, air bag storehouse is corresponded on movable plate equipped with several gaskets, the left and right sides of movable plate is equipped with sliding block, sliding slot is correspondingly provided on the inside of box cover, movable plate is connect with box cover by several springs, the utility model to detect for DNA methylation each Reagent can stored properly, and this kit can direct express transportation, it is low in cost.
Description
Technical field
The utility model relates to molecular biology fields, and in particular to a kind of DNA methylation detection kit.
Background technique
DNA methylation is that apparently one of modification mode, the methylation in eucaryote occur only at for the gene that finds earliest
Cytimidine, i.e., the cytimidine for holding CpG dinucleotides 5 '-are changed into 5 '-
Methylcystein.The usual inhibition of gene expression of DNA methylation, demethylation then reactivating and expressing induction of gene.
A kind of DNA methylation detection kit and its detection method of Publication No. CN103031375B, is wanted in right
Ask and disclose the concrete operation step of DNA methylation detection kit detection DNA methylation in book: preparation has graphene oxidation
The slide of object point battle array, the preparation of DNA sample to be measured, detection probe are adsorbed onto slide with graphene oxide dot matrix, DNA first
Baseization detects, which combines graphene oxide and microarray chip technology, can be high-throughput, inexpensive, accurate, quick
DNA methylation is detected on a large scale.
And a kind of DNA methylation detection kit of Publication No. CN108130359A and its application, the DNA first of the invention
Base detection kit includes CT conversion fluid, antioxidant, in conjunction with liquid, cleaning solution, desulfonate liquid, eluent and suspension containing magnetic beads.
DNA methylation detection kit of the invention recycles DNA fragmentation from modifying DNA solution using magnetic separation technique, has recycling
High-efficient, recycling fragment integrity is good, can be with nucleic acid automatic extracting instrument with the use of the DNA methylation assay for realizing high pass quantization, section
About plenty of time and raising detection efficiency.
Although being actually required to various specific it can be seen that the experimental method of DNA methylation experiment is slightly different
Reagent based on, and be required to a certain amount of DNA sample, and DNA sample has low temperature requirements for storage environment, simultaneously
Road, which jolts, during transportation needs to guarantee the sealing and integrality of reagent, to prevent sample from damaging.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides a kind of DNA methylation detection kit, so that being used for
DNA methylation detection every reagent can properly stored, and this kit can direct express transportation, it is at low cost
It is honest and clean.
(2) technical solution
In order to achieve the above object, the utility model is achieved by the following technical programs:
A kind of DNA methylation detection kit, including reagent box body, the reagent box body is interior equipped with air bag, described
Air bag be it is rectangular, the air bag is made of several air bag storehouses, is communicated between the air bag storehouse, the rear wall of the air bag is equipped with
Air bag nozzle, the air bag are set in spongy layer, and the spongy layer is cylindrical shape, the inner wall of the spongy layer and the air bag
Outer wall form ice bag storehouse, the spongy layer is set in the reagent box body, and groove, institute are equipped in the reagent box body
It states and is equipped with the first screw thread in groove, the reagent box body is mating to be equipped with box cover, and the lower section of the box cover corresponds to the groove and sets
There is convex block, the convex block is equipped with the second screw thread, and the convex block is connected through a screw thread with the groove, is equipped with and lives in the box cover
Movable plate corresponds to the air bag storehouse on the movable plate equipped with several gaskets, and the left and right sides of the movable plate is equipped with sliding block,
Sliding slot is correspondingly provided on the inside of the box cover, the movable plate is connect with the box cover by several springs.
Preferably, the quantity in the air bag storehouse is consistent with the quantity of the gasket.
Preferably, the air bag nozzle is equipped with fixture.
Preferably, the quantity of the spring is more than or equal to 4.
Preferably, the specification of the gasket is 0.8 times of the Hatch Opening specification in the air bag storehouse.
(3) beneficial effect
The utility model provides a kind of DNA methylation detection kit, has the advantages that
Firstly, the storage warehouse of Reagent Tube is designed as air bag storehouse, and to communicate between air bag storehouse, by filling into air bag
Gas is beneficial to form protection environment, will not collide and rub during transportation between the Reagent Tube in effective protection air bag storehouse
It wipes, achievees the purpose that protect reagent.
Secondly, being externally provided with spongy layer in air bag, the ice bag storehouse that one side air bag and spongy layer are formed can place ice bag,
Prolonged low temperature environment is formed in kit, the droplet that ice bag generates in melting process can be absorbed in one side sponge, protects
Hold the dry environment in kit.
Finally, being equipped with movable plate in box cover, and movable plate is connect with box cover by spring, in use, on movable plate
Gasket air bag storehouse is formed to opposite sealed environment, prevent in transportational process jolting to reagent generate breaking-up.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the structural schematic diagram of the utility model box cover；
Fig. 3 is the cross-sectional view of the utility model box cover；
In attached drawing, each label is respectively represented:
1- reagent box body；2- air bag；3- spongy layer；4- groove；5- convex block；6- box cover；7- air bag nozzle；8- air bag
Storehouse；9- ice bag storehouse；10- sliding slot；11- sliding block；12- gasket；13- spring；14- movable plate.
Specific embodiment
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model a part of the embodiment, instead of all the embodiments.Based on the implementation in the utility model
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The range of the utility model protection.
As shown in Figure 1, a kind of DNA methylation detection kit, including reagent box body 1, reagent box body 1 is interior to be equipped with gas
Capsule 2, air bag 2 be it is rectangular, air bag 2 is made of several air bag storehouses 8, is communicated between air bag storehouse 8, and the rear wall of air bag 2 is equipped with gas
Capsule nozzle 7.
In use, first practical pipe can be placed in air bag storehouse 8 due to being communicated between air bag storehouse 8, then by air bag
Nozzle 7 is connect with inflator, pours into air into air bag storehouse 8, so that being filled entirely with air in air bag 2, is realized to four wall of Reagent Tube
Package, after the completion of inflation, air bag nozzle 7 can be knotted, to prevent gas from flowing out.
Wherein, air bag nozzle 7 is equipped with fixture.
Step up air bag nozzle 7 using fixture, fixture and extra air bag nozzle 7 are placed in nearest ice bag storehouse 9, prevented
Fixture is during transportation for the destruction of Reagent Tube.
As shown in Figure 1, air bag 2 is set in spongy layer 3, spongy layer 3 is cylindrical shape, inner wall and the air bag 2 of spongy layer 3
Outer wall forms ice bag storehouse 9, and spongy layer 3 is set in reagent box body 1.
In use, the good ice bag of ice can be placed in ice bag storehouse 9, since what is squeezed into air bag 2 is air, have no effect on
Ice bag will be cooled to low temperature environment in air bag storehouse 8, and during transportation, ice bag becomes water from ice, and ice bag surface is easy to appear
Droplet, spongy layer 3 absorb the droplet on ice bag surface while insulation, keep the dry environment in kit.
As shown in Figure 1, being equipped with groove 4 in reagent box body 1, the first screw thread is equipped in groove 4, reagent box body 1 is mating
Equipped with box cover 6, the lower section respective slot 4 of box cover 6 is equipped with convex block 5, and convex block 5 is equipped with the second screw thread, and convex block 5 passes through with groove 4
It is threadedly coupled.
In use, the convex block 5 of the second screw thread of band can be screwed in the groove 4 of the first screw thread of band, box cover 6 and kit sheet are realized
The seamless connection of body 1.
As shown in Fig. 2, being equipped with movable plate 14 in box cover 6, air bag storehouse 8 is corresponded on movable plate 14 equipped with several gaskets
12。
As shown in figure 3, the left and right sides of movable plate 14 is equipped with sliding block 11, the inside of box cover 6 is correspondingly provided with sliding slot 10, activity
Plate 14 is connect with box cover 6 by several springs 13, and the quantity of spring 13 is more than or equal to 4.
In use, since the sliding block 11 on movable plate 14 is arranged in sliding slot 10, when box cover 6 rotates, movable plate
14 rotate immediately, and as convex block 5 gradually submerges in groove 4, movable plate 14 is contacted with air bag 2, and spring 13 is compressed at this time, work as work
When gasket 12 on movable plate 14 is caught in the Hatch Opening in air bag storehouse 8 in rotary course, stop rotating box cover 6, at this time due to spring
13 are compressed always, will give movable plate 14 downward power, it is made to form a degree of sealing with air bag 2, reach protection gas
The purpose of Reagent Tube in capsule storehouse 8.
Wherein, the quantity in air bag storehouse 8 is consistent with the quantity of gasket 12, and the specification of gasket 12 is the storehouse in air bag storehouse 8
0.8 times of mouth specification.
Be beneficial to movable plate 14 combined with air bag 2 it is even closer.
One specific embodiment of the utility model are as follows:
Reagent Tube can be individually positioned in different air bag storehouses 8, inflator be connect with air bag nozzle 7, into air bag 2
Air is squeezed into, wraps up it by the outer wall of Reagent Tube, realizes the fixation for Reagent Tube.
Air bag nozzle 7 is knotted or is stepped up using fixture, the good ice bag of ice is placed in ice bag storehouse 9, holds box cover
6, convex block 5 is placed in groove 4, and is rotated down, until stopping rotating when gasket 12 is caught in the Hatch Opening in air bag storehouse 8
Box cover 6 after the completion of operation, that is, is realized the storage for Reagent Tube, can also directly be posted.
Rotation box cover 6 is reversely rotated, box cover 6 is opened, when the opening of air bag nozzle 7, releases the partial air in air bag 2,
Reagent Tube and ice bag are taken out.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
Above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；Although referring to aforementioned reality
Example is applied the utility model is described in detail, those skilled in the art should understand that: it still can be to preceding
Technical solution documented by each embodiment is stated to modify or equivalent replacement of some of the technical features；And these
It modifies or replaces, the spirit and model of various embodiments of the utility model technical solution that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (5)
1. a kind of DNA methylation detection kit, which is characterized in that including reagent box body (1), the reagent box body (1)
It is interior be equipped with air bag (2), the air bag (2) be it is rectangular, the air bag (2) is made of several air bag storehouses (8), the air bag storehouse
(8) it being communicated between, the rear wall of the air bag (2) is equipped with air bag nozzle (7), and the air bag (2) is set in spongy layer (3),
The spongy layer (3) is cylindrical shape, and the inner wall of the spongy layer (3) and the outer wall of the air bag (2) form ice bag storehouse (9), institute
It states spongy layer (3) to be set in the reagent box body (1), is equipped with groove (4) in the reagent box body (1), the groove
(4) the first screw thread is equipped in, the reagent box body (1), which is matched, to be arranged with box cover (6), described in the lower section correspondence of the box cover (6)
Groove (4) is equipped with convex block (5), and the convex block (5) is equipped with the second screw thread, and the convex block (5) and the groove (4) pass through screw thread
Connection, the box cover (6) is interior to be equipped with movable plate (14), and the air bag storehouse (8) is corresponded on the movable plate (14) equipped with several
The left and right sides of gasket (12), the movable plate (14) is equipped with sliding block (11), is correspondingly provided with sliding slot on the inside of the box cover (6)
(10), the movable plate (14) is connect with the box cover (6) by several springs (13).
2. DNA methylation detection kit as described in claim 1, which is characterized in that the quantity of the air bag storehouse (8) and institute
The quantity for stating gasket (12) is consistent.
3. DNA methylation detection kit as described in claim 1, which is characterized in that the air bag nozzle (7) is equipped with folder
Tool.
4. DNA methylation detection kit as described in claim 1, which is characterized in that the quantity of the spring (13) is greater than
Equal to 4.
5. DNA methylation detection kit as described in claim 1, which is characterized in that the specification of the gasket (12) is
0.8 times of the Hatch Opening specification of the air bag storehouse (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821612621.5U CN208979498U (en) | 2018-09-30 | 2018-09-30 | A kind of DNA methylation detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821612621.5U CN208979498U (en) | 2018-09-30 | 2018-09-30 | A kind of DNA methylation detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208979498U true CN208979498U (en) | 2019-06-14 |
Family
ID=66786305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821612621.5U Expired - Fee Related CN208979498U (en) | 2018-09-30 | 2018-09-30 | A kind of DNA methylation detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208979498U (en) |
-
2018
- 2018-09-30 CN CN201821612621.5U patent/CN208979498U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230116 Address after: 430000 Hubei city of Wuhan province Wuchang Luojiashan Patentee after: WUHAN University Address before: 430000 room d107, 4th floor, building B7, Guanggu Biological Innovation Park, No. 666, Gaoxin Avenue, Donghu New Technology Development Zone, Wuhan, Hubei Patentee before: AKASITE (WUHAN) BIOTECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190614 |
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| CF01 | Termination of patent right due to non-payment of annual fee |