CN218115421U

CN218115421U - Proteomics sample exosome separation device

Info

Publication number: CN218115421U
Application number: CN202222107181.0U
Authority: CN
Inventors: 吴秀娟; 陆晟; 陈辰; 陈亚琳; 胡程
Original assignee: SHANGHAI XUHUI DISTRICT CENTRAL HOSPITAL
Current assignee: SHANGHAI XUHUI DISTRICT CENTRAL HOSPITAL
Priority date: 2022-08-11
Filing date: 2022-08-11
Publication date: 2022-12-23
Anticipated expiration: 2032-08-11

Abstract

The utility model relates to a proteomics sample exosome separator, including the stock solution frame, stock solution frame top one side is rotated and is connected with the apron, the first electric telescopic handle of apron top center fixedly connected with, the end of first electric telescopic handle is rotated and is connected with the joint that floats, the joint bottom fixedly connected with upper fixed frame that floats, the inside separator tube that is equipped with of upper fixed frame, separator tube bottom fixedly connected with fixing base, fixing base bottom fixedly connected with lower fixed frame, stock solution frame one side fixedly connected with extension board, extension board top fixedly connected with washs the case, the utility model discloses a set up the pump body and can carry the washing liquid of wasing incasement portion to the washing spray tube, can adjust the spray angle who washs the spray tube through setting up third electric telescopic handle, wash the spray tube and use with the cooperation of rotary box and be convenient for carry out all-round washing to the separator tube after the separation is accomplished, avoid having the vesicle to block up the inside isolating hole of separator tube.

Description

Proteomics sample exosome separation device

Technical Field

The utility model relates to a separator specifically is a proteomics sample exosome separator, belongs to biotechnology technical field.

Background

The exosome refers to a disc-shaped vesicle with the diameter of 40-100nm, various cells can secrete the exosome under normal and pathological states, the exosome is mainly derived from a multi-vesicle body formed by invagination of intracellular lysosome particles, the multi-vesicle body is released into an extracellular matrix after fusion of an outer membrane and a cell membrane of the multi-vesicle body, and the exosome needs to be subjected to separation operation of macromolecular liquid and micromolecular liquid.

When current separator separates the exosome, the separator top majority does not have the pressure tight measure, and unbalance phenomenon easily appears in the dynamic balance of separator when high-speed the rotation, and after the separator separates the exosome, the inside separation hole of separator easily appears blocking phenomenon simultaneously, needs to be washed by staff's manual, wastes time and energy, has increased staff intensity of labour.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a proteomics sample exosome separator just for solving above-mentioned problem, can carry the washing liquid that washs incasement portion to washing spray tube through setting up the pump body, can adjust the spray angle who washs the spray tube through setting up third electric telescopic handle, wash the spray tube and use with rotatory case cooperation and be convenient for carry out all-round washing to the cylinder after the separation is accomplished, avoid having the vesicle to block up the inside separation hole of cylinder.

The utility model discloses a following technical scheme realizes above-mentioned purpose, a proteomics sample exosome separator, including the stock solution frame, stock solution frame top one side is rotated and is connected with the apron, the first electric telescopic handle of the fixedly connected with of apron top center department, the end rotation of first electric telescopic handle is connected with the joint that floats, the joint bottom fixedly connected with upper fixed frame floats, the inside separator bowl that is equipped with of upper fixed frame, separator bowl bottom fixedly connected with fixing base, fixing base bottom fixedly connected with lower fixed frame, stock solution frame one side fixedly connected with extension board, extension board top fixedly connected with washs the case.

Preferably, apron one side fixedly connected with C word frame, the apron top is close to C word frame one side fixedly connected with handle, C word frame threaded connection has adjusting bolt, adjusting bolt is terminal to be contradicted with stock solution frame inner wall and is connected.

Preferably, the bottom of the liquid storage frame is fixedly connected with a workbench, the center of the top of the workbench is fixedly connected with a rotary box, a servo motor is fixedly connected inside the rotary box, the output end of the servo motor is fixedly connected with a first bevel gear, the first bevel gear is meshed with a second bevel gear, the center of the second bevel gear is fixedly connected with a transmission shaft, the bottom of the transmission shaft is rotatably connected with the inner wall of the rotary box, and the top of the transmission shaft is fixedly connected with the center of the bottom of the lower fixed frame.

Preferably, stock solution frame inner wall fixedly connected with guide plate, guide plate bottom and workstation top fixed connection, workstation bottom intercommunication is connected with the discharge pipe, fixedly connected with choke valve on the discharge pipe, fixed frame both sides fixedly connected with second electric telescopic handle down, the terminal fixedly connected with grip block of second electric telescopic handle, the grip block is contradicted with the fixing base lateral wall and is connected.

Preferably, a collecting box is arranged below the discharge pipe, the bottom of the collecting box is connected with a bottom plate in a sliding mode, the top of the bottom plate is fixedly connected with a supporting rod, and the top of the supporting rod is fixedly connected with the bottom of the workbench.

Preferably, the top of the cleaning box is fixedly connected with a pump body, the top of the pump body is fixedly connected with a liquid supply pipeline, the tail end of the liquid supply pipeline is communicated and connected with a hose, and the tail end of the hose is fixedly connected with a cleaning spray pipe.

Preferably, washing spray tube top one side is rotated and is connected with branch, the branch end is rotated with the stock solution frame inner wall and is connected, washing spray tube bottom one side is rotated and is connected with third electric telescopic handle, third electric telescopic handle end is rotated with stock solution frame inner wall and is connected.

The beneficial effects of the utility model are that:

the utility model discloses a set up adjusting bolt and be convenient for with C word frame and stock solution frame fixed connection, it takes place to become flexible to avoid the cover plate in the separation process, influence the separation effect, can drive the upper fixed frame through setting up first electric telescopic handle and go up and down, after carrying out the liquid feeding to the separator tube, the controllable upper fixed frame of first electric telescopic handle pushes down, be convenient for fix the separator tube top, can drive the grip block through setting up second electric telescopic handle and remove, be convenient for stabilize the centre gripping to the fixing base, avoid the separator tube to take place to rock in rotatory process, can carry out the high rotation to the separator tube through setting up the rotatory case, be convenient for separate exosome, can carry the washing liquid that washs incasement portion to the washing spray tube through setting up the pump body, can adjust the spray angle who washs the spray tube through setting up third electric telescopic handle, wash the spray tube and use with the cooperation of rotatory case and be convenient for carry out all-round washing to the separator tube after the separation is accomplished, avoid having the vesicle to block up the inside separation hole of separator tube, influence next result of use.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic view of the internal structure of the rotary box of the present invention.

Fig. 3 is an enlarged view of the position a of the present invention.

The reference numbers in the figures: 1. a liquid storage frame; 2. a cover plate; 3. a first electric telescopic rod; 4. an upper fixing frame; 5. a separation cylinder; 6. a fixed seat; 7. a lower fixed frame; 8. a cleaning tank; 9. a C-shaped frame; 10. a rotating box; 11. a servo motor; 12. a drive shaft; 13. a discharge pipe; 14. a collection box; 15. a pump body; 16. cleaning the spray pipe; 17. a third electric telescopic rod; 18. a second electric telescopic rod.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

Please show that please refer to fig. 1-3, a proteomics sample exosome separator, including

stock solution frame

1, 1 top one side of stock solution frame is rotated and is connected with

apron

2, 2 first electric telescopic handle 3 of 2 top center departments fixedly connected with of apron, 3 end-to-end rotations of first electric telescopic handle are connected with the joint that floats, the joint bottom fixedly connected with upper fixed frame 4 that floats, the inside separating cylinder 5 that is equipped with of upper

fixed frame

4, 5 bottom fixedly connected with fixing bases 6 of separating cylinder, 6 bottom fixedly connected with lower fixed frames 7 of fixing base, 1 one side fixedly connected with extension board of stock solution frame, extension board top fixedly connected with washs case 8.

Specifically, a C-shaped frame 9 is fixedly connected to one side of the cover plate 2, a handle is fixedly connected to one side of the cover plate 2 close to the C-shaped frame 9, an adjusting bolt is connected to the C-shaped frame 9 through a thread, the end of the adjusting bolt is connected to the inner wall of the liquid storage frame 1 in a butting manner, a working table is fixedly connected to the bottom of the liquid storage frame 1, a rotary box 10 is fixedly connected to the center of the top of the working table, a servo motor 11 is fixedly connected to the inside of the rotary box 10, a first bevel gear is fixedly connected to the output end of the servo motor 11, a second bevel gear is connected to the first bevel gear in a meshing manner, a transmission shaft 12 is fixedly connected to the center of the second bevel gear, the bottom of the transmission shaft 12 is rotatably connected to the inner wall of the rotary box 10, a guide plate is fixedly connected to the inner wall of the liquid storage frame 1, the bottom of the guide plate is fixedly connected to the top of the working table, a discharge pipe 13 is connected to the bottom of the working table, a throttle valve is fixedly connected to the discharge pipe 13, two sides of the lower fixed frame 7 are fixedly connected to a second electric telescopic rods 18, a clamp plate is fixedly connected to the end of the second electric telescopic rod 18, a hose is connected to the top of the bottom of the rotary box, a cleaning support rod is connected to the top of the cleaning support rod 16, and a cleaning hose is connected to the bottom of the cleaning hose is connected to the top of the pump body, the branch end is connected with 1 inner wall of stock solution frame rotation, it is connected with third electric telescopic handle 17 to wash 16 bottom one side rotation of spray tube, the end of third electric telescopic handle 17 rotates with 1 inner wall of stock solution frame to be connected, be convenient for with

C word frame

9 and 1 fixed connection of stock solution frame through setting up adjusting bolt, avoid among the separation process apron 2 to take place not hard up, influence the separation effect, can drive upper fixed frame 4 through setting up first electric telescopic handle 3 and go up and down, after carrying out the liquid feeding to separation tube 5, first electric telescopic handle 3 can control upper fixed frame 4 and push down, be convenient for fix 5 tops of separation tube, can drive the grip block through setting up second electric telescopic handle 18 and move, be convenient for stabilize the centre gripping to fixing base 6, avoid separation tube 5 to take place to rock in rotatory process, can carry out high rotation to separation tube 5 through setting up rotatory case 10, be convenient for separate the exosome, can carry the inside washing liquid of washing tank 8 to washing spray tube 16 through setting up pump body 15, can accomplish the all-round separation effect of separation tube 5 after the washing, the separation tube 16 has the effect of the influence of the separation tube 5, the separation effect of the full-round separation of using of the effect of using of the separation tube.

The utility model discloses when using, be convenient for with

C word frame

9 and 1 fixed connection of stock solution frame through setting up adjusting bolt, it is not hard up to avoid taking place of apron 2 among the disengaging process, influence the separation effect, can drive upper fixed frame 4 through setting up first electric telescopic handle 3 and go up and down, after carrying out the liquid feeding to separator 5, first electric telescopic handle 3 controllable upper fixed frame 4 pushes down, be convenient for fix separator 5 top, can drive the grip block through setting up second electric telescopic handle 18 and remove, be convenient for stabilize the centre gripping to fixing base 6, avoid separator 5 to take place to rock at rotatory in-process, can carry out the high rotation to separator 5 through setting up rotatory case 10, be convenient for separate the exosome, can carry the inside washing liquid of washing case 8 to washing spray tube 16 through setting up pump body 15, can adjust the jet angle who washs spray tube 16 through setting up third electric telescopic handle 17, wash spray tube 16 and rotatory case 10 cooperation use be convenient for wash separator 5 after the separation is accomplished, avoid having blockked up the inside separation hole of separator 5, influence next time result of use.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and therefore, the present invention is considered to be exemplary and not restrictive in any way, since the scope of the present invention is defined by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, without any reference thereto being construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A proteomics sample exosome separation device comprises a liquid storage frame (1), and is characterized in that: stock solution frame (1) top one side is rotated and is connected with apron (2), apron (2) top center department fixedly connected with first electric telescopic handle (3), first electric telescopic handle (3) end-to-end rotation is connected with the joint that floats, the joint bottom fixedly connected with upper fixed frame (4) float, the inside separator tube (5) that is equipped with of upper fixed frame (4), separator tube (5) bottom fixedly connected with fixing base (6), fixing base (6) bottom fixedly connected with lower fixed frame (7), stock solution frame (1) one side fixedly connected with extension board, extension board top fixedly connected with washs case (8).

2. The proteomic sample exosome separation device according to claim 1, wherein: apron (2) one side fixedly connected with C word frame (9), apron (2) top is close to C word frame (9) one side fixedly connected with handle, C word frame (9) threaded connection has adjusting bolt, adjusting bolt is terminal to be contradicted with stock solution frame (1) inner wall and is connected.

3. The proteomic sample exosome separation device according to claim 1, wherein: stock solution frame (1) bottom fixedly connected with workstation, workstation top center department fixedly connected with rotatory case (10), inside fixedly connected with servo motor (11) of rotatory case (10), servo motor (11) output end fixedly connected with first bevel gear, first bevel gear meshing is connected with second bevel gear, second bevel gear axle center department fixedly connected with transmission shaft (12), transmission shaft (12) bottom and rotatory case (10) inner wall rotate to be connected, transmission shaft (12) top and fixed frame (7) bottom center department fixed connection down.

4. The proteomic sample exosome separation device according to claim 3, wherein: stock solution frame (1) inner wall fixedly connected with guide plate, guide plate bottom and workstation top fixed connection, workstation bottom intercommunication is connected with discharge pipe (13), fixedly connected with choke valve on discharge pipe (13), fixed frame (7) both sides fixedly connected with second electric telescopic handle (18) down, second electric telescopic handle (18) end fixedly connected with grip block, the grip block is contradicted with fixing base (6) lateral wall and is connected.

5. The proteomic sample exosome separation device according to claim 4, wherein: a collecting box (14) is arranged below the discharge pipe (13), the bottom of the collecting box (14) is connected with a bottom plate in a sliding mode, the top of the bottom plate is fixedly connected with a supporting rod, and the top of the supporting rod is fixedly connected with the bottom of the workbench.

6. The proteomic sample exosome separation device according to claim 1, wherein: the cleaning device is characterized in that a pump body (15) is fixedly connected to the top of the cleaning box (8), a liquid supply pipeline is fixedly connected to the top of the pump body (15), the tail end of the liquid supply pipeline is communicated and connected with a hose, and a cleaning spray pipe (16) is fixedly connected to the tail end of the hose.

7. The proteomic sample exosome separation device according to claim 6, wherein: wash spray tube (16) top one side and rotate and be connected with branch, the branch end rotates with stock solution frame (1) inner wall to be connected, wash spray tube (16) bottom one side and rotate and be connected with third electric telescopic handle (17), third electric telescopic handle (17) end rotates with stock solution frame (1) inner wall to be connected.