CN218115417U - Device for insulin cell in-vitro sugar stimulation - Google Patents
Device for insulin cell in-vitro sugar stimulation Download PDFInfo
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- CN218115417U CN218115417U CN202221343229.1U CN202221343229U CN218115417U CN 218115417 U CN218115417 U CN 218115417U CN 202221343229 U CN202221343229 U CN 202221343229U CN 218115417 U CN218115417 U CN 218115417U
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- peristaltic pump
- sugar stimulation
- cell
- side feed
- islet cells
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Abstract
The utility model relates to the technical field of biological experimental devices, and discloses a device for insulin cell in-vitro sugar stimulation, which comprises a perfusion tank and a peristaltic pump, wherein the perfusion tank and the peristaltic pump are connected end to end through a conduit; fill jar and be equipped with side feed inlet, fill jar interior both sides that correspond side feed inlet and be equipped with the screen cloth respectively. The utility model discloses a peristaltic pump makes liquid flow at the device inner loop, and the true state of simulation cell in vivo improves the accuracy height that whether the detection islet cell has insulin secretion function, verifies the islet cell quality.
Description
Technical Field
The utility model relates to a biological experimental apparatus technical field, concretely relates to be used for the amazing device of the external sugar of islet cells.
Background
The islet cell in-vitro sugar stimulation experiment is usually carried out in a culture dish, and since the islet cells and culture solution in the culture dish are in a static state, which is different from the real state of in-vivo cell flow, the experimental effect cannot reflect the insulin secretion function of the real islet cells, and the quality of the islet cells cannot be verified.
SUMMERY OF THE UTILITY MODEL
In order to solve the defects in the prior art, the utility model provides a device for the in vitro sugar stimulation of islet cells.
In order to realize the technical purpose, the utility model discloses a technical scheme is:
a device for in-vitro sugar stimulation of islet cells comprises a perfusion tank and a peristaltic pump, wherein the perfusion tank and the peristaltic pump are connected end to end through a conduit; fill jar and be equipped with side feed inlet, fill in jar and be equipped with the screen cloth respectively to side feed inlet both sides.
Furthermore, a three-way connecting pipe is arranged on the guide pipe, and the other port of the three-way connecting pipe is used for filling liquid or sucking liquid in the circulating process.
Further, the peristaltic pump is a temperature-controlled peristaltic pump.
Further, the peristaltic pump is provided with an air inlet, and the air inlet is connected with a carbon dioxide gas source with the concentration of 5%.
Further, the peristaltic pump is adjustable in rotation speed.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model promotes the liquid to circularly flow in the device through the peristaltic pump, simulates the real state of cells in the body, and has high accuracy in detecting the insulin secretion function of islet cells; furthermore, the device simulates the real environment of cells in the body, provides carbon dioxide with proper temperature and proper concentration, eliminates the influence of environmental factors and further improves the accuracy of simulation experiment results.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Reference numerals: 1-filling tank, 2-peristaltic pump, 3-conduit, 4-side feeding port, 5-screen, 6-three-way connecting pipe and 7-air inlet.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, as generally described and illustrated in the figures herein, could be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The utility model provides a device for in vitro sugar of islet cells is amazing, is including filling jar 1, peristaltic pump 2, fill jar 1 and 2 end to end through 3 pipes of peristaltic pump, fill jar 1 still is equipped with side feed opening 4, and 4 both sides of corresponding side feed opening are equipped with screen cloth 5 respectively in filling jar 1, and during the experiment, islet cells pours into from side feed opening 4, arranges in the middle of two screen cloth 5. The peristaltic pump enables liquid to flow in a circulating mode in the whole device, and the peristaltic pump can adjust the rotating speed to obtain different perfusion rates according to the difference of the volume size of the perfusion tank and the concentration of cells.
Furthermore, a three-way connecting pipe 6 is arranged on the catheter 3, and the other port of the three-way connecting pipe 6 is used for filling liquid or sucking liquid in the circulation process for detection.
Preferably, the peristaltic pump 2 is a temperature-controlled peristaltic pump, and can heat and control the temperature of the circulating liquid to control the temperature within the range of 37 +/-1 ℃, and further, the peristaltic pump is provided with an air inlet which is connected with a carbon dioxide gas source with the concentration of 5%, so that the carbon dioxide with the concentration of 5% enters the circulating channel to simulate the environment of cells in the body.
The device is used for detecting the in-vitro sugar stimulation secretion experiment of the islet cells, simulates the real state of the islet cells in vivo, provides carbon dioxide and blood fluidity with proper temperature and proper concentration, eliminates the influence of environmental factors, and improves the accuracy of detecting whether the islet cells have an insulin secretion function and verifying the quality of the islet cells.
Of course, the present invention can be embodied in many other forms without departing from the spirit or essential attributes thereof, and it is intended that all such modifications and variations be considered within the spirit or scope of the present invention as defined by the appended claims.
Claims (5)
1. A device for ex vivo sugar stimulation of islet cells, characterized by: the device comprises a perfusion tank (1) and a peristaltic pump (2), wherein the perfusion tank (1) is connected with the peristaltic pump (2) end to end through a conduit (3); fill jar (1) and be equipped with side feed inlet (4), fill jar (1) and interior corresponding side feed inlet (4) both sides and be equipped with screen cloth (5) respectively.
2. The device for ex vivo sugar stimulation of pancreatic islet cells of claim 1, characterized in that: and a three-way connecting pipe (6) is arranged on the catheter (3), and the other port of the three-way connecting pipe (6) is used for filling liquid or sucking liquid in the circulating process.
3. The device for islet cell in vitro sugar stimulation according to claim 1, characterized in that: the peristaltic pump (2) is a temperature-control peristaltic pump.
4. The device for ex vivo sugar stimulation of pancreatic islet cells of claim 3, characterized in that: the peristaltic pump (2) is provided with an air inlet (7), and the air inlet (7) is connected with a carbon dioxide gas source with the concentration of 5%.
5. The device for islet cell in vitro sugar stimulation according to claim 1, characterized in that: the rotating speed of the peristaltic pump (2) is adjustable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221343229.1U CN218115417U (en) | 2022-05-31 | 2022-05-31 | Device for insulin cell in-vitro sugar stimulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221343229.1U CN218115417U (en) | 2022-05-31 | 2022-05-31 | Device for insulin cell in-vitro sugar stimulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218115417U true CN218115417U (en) | 2022-12-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221343229.1U Active CN218115417U (en) | 2022-05-31 | 2022-05-31 | Device for insulin cell in-vitro sugar stimulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218115417U (en) |
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2022
- 2022-05-31 CN CN202221343229.1U patent/CN218115417U/en active Active
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