CN218115406U - A cell processing device - Google Patents

Abstract

The utility model discloses a cell processing device, which comprises a driving part, a connecting pipe and an extruding part. The connecting pipe connects the driving part and the extruding part. Department, for extrusion processing. The driving part includes a piston and an outer body containing the piston, the outer side of the piston is connected to the piston handle, and the reciprocating movement of the piston is controlled by manually or mechanically pushing and pulling the piston handle. The extruded part is a constricted section. The utility model provides a device for extruding cells through the structure of the contraction section and providing mechanical stimulation. The width of the contraction section can be fine-tuned; the scheme of using the piston as the driving force can realize the extrusion of cells through the structure of the contraction section for many times. The process can be repeated many times, and the width of the contraction section can also be fine-tuned according to the demand; compared with the operation of replacing the chip in the microfluidic channel method of the prior art, the device has a simple structure and strong practicability.

Description

A cell processing device

technical field

The utility model relates to the technical field of biomedical cell processing, in particular to a cell processing device.

Background technique

Cell extrusion is one of the ways of cell pretreatment. Physical squeezing of cells and their contents can cause cells to grow and divide faster than normal, the study found. The Massachusetts Institute of Technology reported in the journal Cell Stem Cell in 2020 that simply squeezing cells can promote the "stemization" of pretreated cells, opening up new ideas for organ-like culture and organ transplantation for regenerative medicine. In addition, cell extrusion briefly squeezes small openings in the cell, which, although lasting only a few seconds, efficiently transport proteins and other molecules into the cell. Patent CN107109362A uses microfluidic channels to extrude immune cells to achieve intracellular delivery of molecules. At present, in order to study the impact of physical extrusion on cells, researchers use different methods such as gel and microfluidics to squeeze different types of cells. However, the gel extrusion method cannot accurately control the compressed volume; although the microfluidic channel method can control the compressed volume of cells through channels of different widths, the microfluidic chip used in this method is expensive, cumbersome to prepare, and the same chip Channels cannot be fine-tuned, possibly compromising the integrity of the nuclear membrane. The contents flowing out after the nuclear membrane ruptures will be mixed with the cytoplasm. The enzymes contained in the cytoplasm can damage the DNA of the cell and seriously affect the survival of the cell. Therefore, how to control the compression volume ratio and simplify the operation steps in the cell extrusion process is one of the technical problems to be solved urgently.

Contents of the invention

The purpose of this utility model is to provide a device for cell extrusion pretreatment, so that at least one of the shortcomings of the prior art is eliminated or at least reduced. The utility model provides the following technical solutions:

A cell processing device, comprising a driving part, a connecting tube and an extruding part, the connecting tube connects the driving part and the extruding part, and the cells to be treated are squeezed through the connecting tube through the extruding part under the action of the driving force provided by the driving part deal with.

Preferably, the driving part includes a piston and an outer body for accommodating the piston, the outer side of the piston is connected to the piston handle, and the reciprocating movement of the piston is controlled by manually or mechanically pushing and pulling the piston handle.

Preferably, the extruded portion is a cured shrinkage section, and the shape of the shrinkage section is selected from a circle, an ellipse or a rectangle.

Preferably, the inner diameter of the constricted section ranges from 0.1 to 1 μm, preferably 0.1 μm.

As one of the methods, the shrinkage section is cured and shaped for the hose.

Preferably, the extruding part includes a fine-tuning device for adjusting the inner diameter of the hose provided at the peripheral constriction section of the hose.

Preferably, the fine-tuning device is selected from clamps, outer micrometers or other structures that can provide quantitative distance pressure.

Preferably, the piston handle of the driving part is connected to the electric push rod to provide a driving force for precise displacement.

Preferably, the cell extrusion processing device includes two sets of driving parts, which are symmetrically arranged on both sides of the extrusion part and connected by connecting pipes.

Preferably, the cell extrusion treatment device includes a set of driving parts, one end of the connecting tube is connected to the driving part, and the other end is connected to a non-driving structure, and the non-driving structure is selected from a volume-fixed container, and the inside of the volume-fixed container Pre-set cell repair reagents.

Preferably, the volume-fixed container is selected from centrifuge tubes.

Compared with the prior art, the beneficial effects of the utility model are:

1. The utility model provides a device for extruding cells and providing mechanical stimulation through the contraction section. The contraction section can have a fixed inner diameter or an adjustable inner diameter. It is equipped with a fine-tuning device for adjusting the structural width of the contraction section to adjust the inner diameter of the contraction section. Precise regulation; can control the compression volume ratio during cell extrusion;

2. The utility model provides a scheme of using the piston as the driving force, which can realize the extrusion of the cells through the structure of the contraction section for many times. This process can be repeated many times, and the inner diameter of the contraction section can also be fine-tuned according to the demand;

3. Compared with the operation of replacing the chip in the microfluidic channel method of the prior art, the device has a simple structure and strong practicability.

Description of drawings

The accompanying drawings constituting a part of the utility model are used to provide a further understanding of the utility model, and the schematic embodiments of the utility model and their descriptions are used to explain the utility model and do not constitute improper limitations to the utility model.

Fig. 1 is a schematic structural diagram of a cell processing device provided in Example 1 of the present invention;

Fig. 2 is a schematic structural view of another cell processing device provided in Example 1 of the present invention;

Fig. 3 is a schematic structural diagram of a cell processing device provided in Example 2 of the present invention;

Fig. 4 is a schematic structural diagram of a cell processing device provided in Example 3 of the present invention.

Among them, 1-piston, 2-piston handle, 3-piston sleeve, 4-hose, 5-shrink section, 6-inlet and outlet interface, 7-centrifuge tube, 8-clamp, 9-outer micrometer, 91- Micrometer screw, 92-anvil.

detailed description

It should be pointed out that the following detailed descriptions are all exemplary and are intended to provide further description of the present utility model. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It should be noted that the terminology used here is only used to describe specific embodiments, and is not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and/or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and/or combinations thereof.

Embodiment 1: A kind of cell processing device

As shown in Figure 1, it includes a symmetrically arranged piston 1, a piston handle 2, and a piston sleeve 3, the two piston sleeves 3 are connected by a hose 4, and a shrinkage section 5 is arranged on the hose 4 between the piston sleeves 3; The piston sleeve 3 and the piston 1 respectively form chambers A and B that can accommodate cells; the shrinkage section 5 on the hose 4 can use shrinking tools such as a heat gun to shrink the inner diameter of the hose, or use other existing methods to cure Formed.

Liquid inlet and outlet ports 6 are respectively provided between the piston sleeves 3 and the contraction section 5 on both sides.

Specifically, the piston sleeve 3 and the hose 4 are made of materials with good biocompatibility and no toxicity to cells; the piston inside the piston sleeve 3 pushes the flow of the contents inside the device; the hose 4 connects with A and B through the joint The two cavities are connected.

The shape of the constricted section 5 can be selected from circular, oval or rectangular; circular is preferred.

When in use, move the cell sample into chamber A or chamber B through the liquid inlet and outlet interface 6, push the piston on the same side, so that the cell sample enters chamber B or chamber A on the opposite side through the contraction section 5, push and pull the piston as needed to make the cell sample Repeatedly enters and exits in chamber A and chamber B, and is squeezed by the shrinkage section 5, so as to precisely control the compression volume ratio during the extrusion process of the cells. The inner diameter of the constricted section can be selected from 0.1-1 μm, preferably 0.1 μm.

Taking the shape of the contraction segment as a circle as an example to explain, the radius of the cell is represented by r ₁ , and the inner diameter of the contraction segment is represented by r ₂ ;

The formula for calculating the volume V1 of the cell is: ;

The formula for calculating the volume V2 of a sphere with the same radius at the contraction section is: ;

Then the compression volume ratio is V2/V1. Through the derivation process of the formula, it can be seen that the compressed volume of the extruded cells can be precisely controlled by accurately measuring the r ₂ data.

Among them, the power of the piston handle can be the power generated by hand pushing, or it can be the driving force such as electric push rod, so as to precisely control the moving distance of the piston. It can also be driven by negative pressure generated by pulling the piston handle.

As a typical embodiment, as shown in Figure 2, a set of piston 1, piston handle 2, and piston sleeve 3 are centrifuge tubes 7 or other containers with non-driving structure, and cell repair reagents can be placed in advance in the centrifuge tubes or other containers .

Example 2: Cell processing device with adjustable inner diameter of constriction section

In order to increase the versatility of this device and realize the adjustment of the inner diameter of the shrinkage section, a fine-tuning device is arranged on the periphery of the hose or the shrinkage section, as shown in Figure 3, the fine-tuning device is a clip 8, and the hose or Inner diameter at the constricted section. The advantage of this process is that it avoids the operation of replacing the chip in the microfluidic channel method, which is simple and practical.

The clamp can be in various forms, as long as it can reduce the inner diameter of the inner catheter of the clamp, it can be used; calculate the inner diameter data according to the tightening length of the screw on the clamp, so as to obtain the shrinkage volume ratio.

Example 3: Cell processing device with adjustable inner diameter of constriction section

As a typical embodiment, a fine-tuning device is arranged on the periphery of the hose or the shrinkage section, as shown in Figure 4, the fine-tuning device is an outer diameter micrometer 9 or other structures that can provide quantitative distance pressure, and the outer diameter micrometer 9 is a mechanical field. Commonly used equipment, the inner diameter width of the constriction section 5 is adjusted by adjusting and recording the distance between the micrometer screw 91 and the anvil 92 . The width of the constriction is calculated by subtracting twice the hose wall thickness from the thickness of the hose at the constriction. When in use, move the cell sample into chamber A or chamber B through the liquid inlet and outlet interface 6, push the piston on the same side, so that the cell sample enters chamber B or chamber A on the opposite side through the contraction section 5, push and pull the piston as needed to make the cell sample In and out repeatedly in chamber A and chamber B, after being squeezed by the slit.

The above descriptions are only preferred embodiments of the utility model, and are not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present utility model shall be included in the protection scope of the present utility model.

Claims (10)

1. A cell processing device, characterized in that it comprises a driving part, a connecting tube and an extruding part, and the connecting tube connects the driving part and the extruding part; The driving part includes a piston and an outer body for accommodating the piston, and the outer side of the piston is connected to the piston handle; The extruded part is a constricted section. 2. A cell processing device according to claim 1, wherein the shape of the constricted section is selected from a circle, an ellipse or a rectangle. 3 . The cell processing device according to claim 2 , wherein the inner diameter of the constricted section ranges from 0.1 to 1 μm. 4 . 4 . The cell treatment device according to claim 3 , wherein the constriction section is a hard tube that is solidified and shaped. 5 . 5 . The cell processing device according to claim 3 , wherein the extruding part includes a fine-tuning device for adjusting the inner diameter of the hose provided at the peripheral constriction section of the hose. 6 . 6 . The cell processing device according to claim 5 , wherein the fine-tuning device is selected from clamps or outer micrometers. 7 . 7. The cell processing device according to claim 1, wherein the piston handle of the driving part is connected to an electric push rod. 8 . The cell processing device according to claim 1 , characterized in that it comprises two sets of driving parts, symmetrically arranged on both sides of the extrusion part, and connected by connecting pipes. 9. A cell processing device according to claim 1, characterized in that it comprises a set of driving parts, one end of the connecting tube is connected to the driving part, and the other end is connected to the non-driving structure, and the non-driving structure has a fixed volume. container. 10 . The cell processing device according to claim 9 , wherein the volume-fixed container is selected from centrifuge tubes. 11 .

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