CN218115406U - Cell processing device - Google Patents
Cell processing device Download PDFInfo
- Publication number
- CN218115406U CN218115406U CN202222316564.9U CN202222316564U CN218115406U CN 218115406 U CN218115406 U CN 218115406U CN 202222316564 U CN202222316564 U CN 202222316564U CN 218115406 U CN218115406 U CN 218115406U
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- China
- Prior art keywords
- piston
- cell processing
- contraction section
- driving
- processing device
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 230000008602 contraction Effects 0.000 claims abstract description 27
- 238000001125 extrusion Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 48
- 230000006835 compression Effects 0.000 description 8
- 238000007906 compression Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000000633 nuclear envelope Anatomy 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell processing apparatus, including drive division, connecting pipe and extrusion portion, drive division and extrusion portion are connected to the connecting pipe, and pending cell passes through extrusion portion by the connecting pipe under the drive power effect that the drive division provided, extrudees the processing. The driving part comprises a piston and an outer sleeve body for accommodating the piston, the outer side of the piston is connected to a piston handle, and the piston handle is pushed and pulled manually or mechanically to control the reciprocating movement of the piston. The extrusion part is a contraction section. The utility model provides a device for extruding cells and providing mechanical stimulation through a contraction section structure, wherein the contraction section can be adjusted by the width of a fine adjustment device; the scheme that the piston is used as the driving force can realize that the cells are extruded by the contraction section structure for multiple times, the process can be repeated for multiple times, and the width of the contraction section can be finely adjusted according to requirements in the process; compared with the operation of replacing the chip in the microfluidic channel method in the prior art, the device has simple structure and strong practicability.
Description
Technical Field
The utility model relates to a biomedical cell processing technology field especially relates to a cell processing apparatus.
Background
Cell compression is one of the ways of cell pretreatment. Physical compression of the cells and their contents has been found to increase the rate of growth and division of the cells beyond normal levels. The Massachusetts ' institute of technology and technology reports that simply squeezing cells can promote the ' stem cell ' of pretreated cells in journal of ' cell stem cell ' in 2020, and opens up a new idea for organ-like culture and regenerative medicine organ transplantation. In addition, cell extrusion can also transiently extrude small openings in cells, albeit only for a few seconds, but effectively transport proteins and other molecules into the cell. Patent CN107109362A uses microfluidic channels to squeeze immune cells to achieve intracellular delivery of molecules. Currently, in order to study the effect of physical compression on cells, researchers use different ways such as gels, microfluidics, etc. to compress different types of cells. However, gel-based extrusion of cells does not allow precise control of the compressed volume; although the microfluidic channel method can control the cell compression volume through channels with different widths, the microfluidic chip used in the method is expensive and complicated to prepare, and the channels of the same chip cannot be finely adjusted, so that the integrity of a nuclear membrane can be damaged. However, the contents discharged after the nuclear membrane is ruptured are mixed with cytoplasm, and the enzyme contained in the cytoplasm can destroy the DNA of the cell, thereby seriously affecting the survival of the cell. Therefore, how to control the compression volume ratio and simplify the operation steps in the cell extrusion process is one of the technical problems to be solved.
Disclosure of Invention
The object of the present invention is to provide a device for cell extrusion pretreatment, which eliminates or at least reduces at least one of the disadvantages of the prior art, and the present invention provides the following technical solutions:
a cell processing device comprises a driving part, a connecting pipe and an extrusion part, wherein the connecting pipe is connected with the driving part and the extrusion part, and cells to be processed are extruded by the connecting pipe through the extrusion part under the action of driving force provided by the driving part.
Preferably, the driving part comprises a piston and an outer sleeve body for accommodating the piston, the outer side of the piston is connected to a piston handle, and the piston handle is manually or mechanically pushed and pulled to control the reciprocating movement of the piston.
Preferably, the extrusion part is a solidified shrinkage section, and the shape of the shrinkage section is selected from circular, oval or rectangular.
Preferably, the inner diameter of the contraction section ranges from 0.1 to 1 μm, and is preferably 0.1 μm.
As one mode, the shrinkage section is used for curing and shaping the hose.
Preferably, the pressing part comprises a fine adjustment device which is arranged at the peripheral contraction section of the hose and is used for adjusting the inner diameter of the hose.
Preferably, the fine tuning device is selected from a clamp, an outside micrometer, or other structure capable of providing a quantitative distance pressure.
Preferably, the piston handle of the driving part is connected to an electric push rod to provide a driving force for accurate displacement.
Preferably, the cell extrusion processing device comprises two sets of driving parts, which are symmetrically arranged at two sides of the extrusion part and are connected through a connecting pipe.
Preferably, the cell extrusion processing device comprises a set of driving part, one end of the connecting pipe is connected with the driving part, the other end of the connecting pipe is connected with a non-driving structure, the non-driving structure is selected from a volume fixing container, and a cell repairing reagent is placed in the volume fixing container in advance.
Preferably, the volume-fixed container is selected from centrifuge tubes.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model provides a device for extruding cells and providing mechanical stimulation through a contraction section, wherein the contraction section can be fixed in inner diameter or adjustable in inner diameter, and a fine adjustment device for adjusting the structural width of the contraction section is arranged to accurately adjust and control the inner diameter of the contraction section; the compression volume ratio in the cell extrusion process can be controlled;
2. the utility model provides a scheme which adopts the piston as the driving force, can realize that the cells are extruded by the contraction section structure for a plurality of times, the process can be repeated for a plurality of times, and the inner diameter of the contraction section can be finely adjusted according to the requirement in the process;
3. compared with the operation of replacing the chip in the microfluidic channel method in the prior art, the device has simple structure and strong practicability.
Drawings
The accompanying drawings, which form a part of the specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to constitute a limitation on the invention.
FIG. 1 is a schematic structural view of a cell processing apparatus according to embodiment 1 of the present invention;
FIG. 2 is a schematic view of another cell processing apparatus according to embodiment 1 of the present invention;
FIG. 3 is a schematic structural view of a cell processing apparatus according to embodiment 2 of the present invention;
fig. 4 is a schematic structural view of a cell processing apparatus according to embodiment 3 of the present invention.
The device comprises a piston 1, a piston handle 2, a piston sleeve 3, a hose 4, a contraction section 5, a liquid inlet and outlet port 6, a centrifuge tube 7, a hoop 8, an external micrometer 9, a micrometer screw 91 and a measuring anvil 92.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
Example 1: cell processing device
As shown in fig. 1, the device comprises a piston 1, a piston handle 2 and piston sleeves 3 which are symmetrically arranged, wherein the two piston sleeves 3 are connected through a hose 4, and a contraction section 5 is arranged on the hose 4 between the piston sleeves 3; a cavity A and a cavity B which can contain cells are respectively formed in the piston sleeve 3 and the piston 1; the contraction section 5 on the hose 4 can be formed by adopting a contraction tool such as a hot air gun to make the local inner diameter of the hose contract or adopting other existing modes for solidification and molding.
And a liquid inlet and outlet interface 6 is respectively arranged between the piston sleeve 3 and the contraction section 5 at the two sides.
Specifically, the piston sleeve 3 and the hose 4 are respectively made of materials which have good biocompatibility and no toxicity to cells; the piston inside the piston sleeve 3 pushes the flow of the contents inside the device; the hose 4 is connected with the cavities A and B through connectors.
The shape of the constriction 5 can be selected from circular, elliptical or rectangular; preferably circular.
During the use, move the cell sample to A chamber or B chamber through business turn over liquid interface 6, promote homonymy piston for the cell sample passes through constriction 5 and gets into contralateral B chamber or A chamber, push and pull the piston as required and make the cell sample pass in and out in A chamber and B intracavity repeatedly, receive the extrusion of constriction 5 and handle, thereby accurate control cell extrusion in-process compression volume ratio. The inner diameter of the constriction may be selected from between 0.1 and 1 μm, preferably 0.1 μm.
The example of the constriction being circular is explained, the radius of the cell being r 1 The inner diameter of the constriction is denoted by r 2 Represents;
the volume of cells, V1, is calculated as: (ii) a
The volume V2 of the sphere with the same radius at the contraction section is calculated by the formula: (ii) a
The compression volume ratio is V2/V1. As can be seen from the derivation of this formula, by accurately measuring r 2 Data, the compressed volume of the squeezed cells was precisely controllable.
The power of the piston handle can be power generated by hand pushing or driving force such as an electric push rod, and the moving distance of the piston is accurately controlled. But also by negative pressure generated by pumping the piston handle.
As a typical example, as shown in FIG. 2, a centrifuge tube 7 or other container in which a set of piston 1, piston handle 2, and piston sleeve 3 are in a non-driven configuration may be pre-loaded with cell repair reagents.
Example 2: cell processing device with adjustable inner diameter of contraction section
In order to increase the universality of the device and realize the adjustment of the inner diameter of the contraction section, a fine adjustment device is arranged on the periphery of the hose or the contraction section, as shown in fig. 3, the fine adjustment device is a clamp 8, and the inner diameter of the hose or the contraction section is adjusted by utilizing the clamp 8. The process has the advantages of avoiding the operation of replacing the chip in the microfluidic channel method, along with simplicity and practicability.
The clamp can adopt various forms, and can be selected as long as the function of reducing the inner diameter of the conduit in the clamp is realized; and calculating the inner diameter data according to the tightening length of the screw on the hoop so as to obtain the contraction volume ratio.
Example 3: cell processing device with adjustable inner diameter of contraction section
As a typical embodiment, a fine adjustment device is arranged on the periphery of the hose or the contracting section, as shown in fig. 4, the fine adjustment device is an outside micrometer 9 or other structures capable of providing quantitative distance pressure, the outside micrometer 9 is a device commonly used in the mechanical field, and the inner diameter width of the contracting section 5 is adjusted by adjusting and recording the distance between the micrometer screw 91 and the anvil 92. The width of the constriction is equal to the thickness of the hose at the constriction minus two times the wall thickness of the hose. During the use, move the cell sample to A chamber or B chamber through business turn over liquid interface 6 in, promote homonymy piston for the cell sample passes through contraction section 5 and gets into contralateral B chamber or A chamber in, push and pull the piston as required and make the cell sample pass in and out in A chamber and B intracavity repeatedly, through the extrusion processing of slit.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A cell processing device is characterized by comprising a driving part, a connecting pipe and an extrusion part, wherein the connecting pipe is connected with the driving part and the extrusion part;
the driving part comprises a piston and an outer sleeve body for accommodating the piston, and the outer side of the piston is connected to the piston handle;
the extrusion part is a contraction section.
2. The cell processing device according to claim 1, wherein the constriction section has a shape selected from the group consisting of a circle, an ellipse and a rectangle.
3. A cell processing device according to claim 2, wherein the inner diameter of the constriction is in the range of 0.1 to 1 μm.
4. The cell processing device according to claim 3, wherein the constriction section is a flexible tube for curing and setting.
5. The device according to claim 3, wherein the pressing portion comprises a fine adjustment device provided at the constricted portion of the outer periphery of the tube for adjusting the inner diameter of the tube.
6. A cell processing device according to claim 5, wherein the fine adjustment means is selected from a clamp or an outside micrometer.
7. The cell processing apparatus according to claim 1, wherein the piston shaft of the driving section is connected to an electric push rod.
8. The cell processing apparatus according to claim 1, comprising two sets of driving portions symmetrically disposed on both sides of the pressing portion and connected by a connecting tube.
9. The cell processing device according to claim 1, comprising a set of driving parts, wherein one end of the connecting tube is connected to the driving part, and the other end is connected to the non-driving structure, and the non-driving structure is a volume fixing container.
10. A cell handling device according to claim 9, wherein the volumetric fixed container is selected from a centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222316564.9U CN218115406U (en) | 2022-09-01 | 2022-09-01 | Cell processing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222316564.9U CN218115406U (en) | 2022-09-01 | 2022-09-01 | Cell processing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218115406U true CN218115406U (en) | 2022-12-23 |
Family
ID=84527930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222316564.9U Expired - Fee Related CN218115406U (en) | 2022-09-01 | 2022-09-01 | Cell processing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218115406U (en) |
-
2022
- 2022-09-01 CN CN202222316564.9U patent/CN218115406U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221223 |
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| CF01 | Termination of patent right due to non-payment of annual fee |