CN218089618U

CN218089618U - Magnetic bead transfer device for high-flux nucleic acid extraction

Info

Publication number: CN218089618U
Application number: CN202221259672.0U
Authority: CN
Inventors: 张健; 张和平; 师丽刚; 刘子璇; 刘坤峰; 姚巧珍; 王华东; 李高品; 袁德林
Original assignee: Henan Sunida Biotechnology Co ltd
Current assignee: Henan Sunida Biotechnology Co ltd
Priority date: 2022-05-23
Filing date: 2022-05-23
Publication date: 2022-12-20
Anticipated expiration: 2032-05-23

Abstract

The utility model discloses a magnetic bead transfer device for high flux nucleic acid extraction relates to nucleic acid testing technical field, specifically is a magnetic bead transfer device for high flux nucleic acid extraction, including the bottom mounting panel, the lower fixed surface of bottom mounting panel is connected with the connecting seat, is equipped with a plurality of threaded connection holes on the connecting seat, and a plurality of threaded connection hole female connection have the transfer pipe, and the last fixed surface of bottom mounting panel is connected with the bracing piece, and the middle part sliding connection of bracing piece has first clamp plate, and the top sliding connection of bracing piece has the second clamp plate, and reset spring has been cup jointed to the bottom of bracing piece, and reset spring's top butt is at the lower surface of first clamp plate. According to the magnetic bead transfer device for high-flux nucleic acid extraction, the bottom mounting plate, the connecting seat, the threaded connecting holes and the transfer pipes are arranged in a matched mode, the transfer pipes can be arranged in the corresponding threaded connecting holes according to the distance between the holes in the used pore plate in the using process, and the using range of the device is expanded.

Description

Magnetic bead transfer device for high-flux nucleic acid extraction

Technical Field

The utility model relates to a nucleic acid detection technical field specifically is magnetic bead transfer device for high flux nucleic acid extraction.

Background

In recent years, the technology of nucleic acid extraction has been rapidly developed, and various nucleic acid extraction instruments have been developed. The biotechnology that targets nucleic acid extraction technology as research is mainly nucleic acid extraction, amplification, and sequencing. Among them, the technology of extracting nucleic acid has been highly regarded as the basis of all other technologies. The quality of the nucleic acid extraction technique directly determines the efficiency of the operation of other subsequent steps and the final quality of the experiment. The magnetic bead method is especially prominently studied in the nucleic acid extraction method. The magnetic bead method extraction steps can be divided into lysis, washing and elution, because the reagents used in different steps have different functions, the volumes of the reagents used in different steps need to be adjusted according to actual needs under the optimal state, and the volumes of the reagents applicable in different steps can be greatly different. However, in the conventional nucleic acid extractor, because of the limitation of the freedom of movement, the transfer of magnetic beads can be synchronously performed only in a column form, and the adjustment of reagent volumes in different steps can only depend on slight differences of reagent addition amounts of pore sites when the pore sites of the pore plate with the same volume are packaged in advance, so that the adjustable range is very small, and ideal different reagent volume changes cannot be realized.

On the other hand, for different kinds of nucleic acid samples, such as FFPE samples and cfDNA samples, although the magnetic bead method can be applied to nucleic acid extraction, due to the characteristics of the samples and other factors, the volume of each well of the well plate used for FFPE extraction is the same, and the reagent amount is different but is small (900 ul-100 ul), while cfDNA extraction is performed on wells with different well plate sizes, and the volume difference between wells is large, and the volume of the added liquid is also large (12 ml-60 ul), so that nucleic acid solution suitable for subsequent detection can be obtained by using nucleic acid extraction reagents with different volumes correspondingly. Therefore, in the prior art, different nucleic acid extractors must be used for different types of nucleic acid samples to perform extraction normally, and the methods are not universal.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

To the not enough of prior art, the utility model provides a magnetic bead transfer device is used in high flux nucleic acid extraction has solved the problem of proposing in the above-mentioned background art.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a magnetic bead transfer device for high flux nucleic acid extraction, includes the bottom mounting panel, the lower fixed surface of bottom mounting panel is connected with the connecting seat, be equipped with a plurality of threaded connection holes on the connecting seat, it is a plurality of threaded connection hole female connection has the transfer pipe, the last fixed surface of bottom mounting panel is connected with the bracing piece, the middle part sliding connection of bracing piece has first clamp plate, the top sliding connection of bracing piece has the second clamp plate, reset spring has been cup jointed to the bottom of bracing piece, reset spring's top butt is at the lower surface of first clamp plate, the outer fixed surface of bracing piece is connected with the baffle, compression spring has been cup jointed at the top of bracing piece, the both ends of bottom mounting panel upper surface are equipped with coupling mechanism, the lower surface of bottom mounting panel is equipped with the protection casing.

Optionally, the number of the threaded connection holes is a plurality, and the positions of the threaded connection holes correspond to different pore plates respectively.

Optionally, the transfer pipe includes a bottom storage rod, a magnetic pipe is inserted into the top of the bottom storage rod, a lower pressing rod is inserted into the top of the magnetic pipe, the top of the magnetic pipe penetrates through the bottom mounting plate and abuts against the lower surface of the first pressing plate, and the lower pressing rod penetrates through the first pressing plate and abuts against the lower surface of the second pressing plate.

Optionally, a guide groove is formed in the side wall of the inner surface of the bottom storage rod, a guide block is fixedly connected to the outer surface of the magnetic tube, the guide block is connected to the inside of the guide groove in a sliding mode, an elastic rope is fixedly connected to the top of the guide groove, and the other end of the elastic rope is fixedly connected to the upper surface of the guide block.

Optionally, a lower push rod is fixedly connected to the lower surface of the lower press rod, a support spring is sleeved on the outer surface of the lower push rod, one end of the support spring abuts against the top of the lower press rod, and the lower push rod penetrates through the magnetic tube.

Optionally, the position of the baffle is located above the first pressure plate, the bottom of the compression spring abuts against the upper surface of the baffle, and the top of the compression spring abuts against the lower surface of the second pressure plate.

Optionally, the connecting mechanism comprises a connecting mounting groove, inner push rods are inserted into two sides of the connecting mounting groove, a connecting spring is arranged between the two inner push rods, a connecting rod is fixedly connected to the lower surface of each inner push rod, and a limiting clamping block is fixedly connected to the bottom of each connecting rod.

Optionally, a limiting groove is formed in the top of the protective cover, one end of the connecting rod penetrates through the bottom mounting plate and is inserted into the limiting groove, and the cross section of the limiting groove is in a T shape.

(III) advantageous effects

The utility model provides a magnetic bead transfer device for high flux nucleic acid extraction possesses following beneficial effect:

1. this magnetic bead transfer device for high flux nucleic acid extraction sets up through the cooperation of bottom mounting panel, connecting seat, threaded connection hole and transfer pipe, can be according to in the in-process of using the interval of each hole on the orifice plate that uses will transfer in the pipe sets up the threaded connection hole that corresponds, has enlarged the application range of this device.

2. This magnetic bead transfer device for high flux nucleic acid extraction, through the transfer tube, the bracing piece, first clamp plate, the second clamp plate, reset spring, baffle and compression spring's cooperation sets up, when the magnetic bead is put into the transfer tube to needs in the in-process of using, thereby downwards press first clamp plate and drive each magnetic tube decline, make its bottom stretch out from bottom deposit pole below, adsorb the magnetic bead to the magnetic tube on, reset spring promotes first clamp plate and resets after loosening first clamp plate, the elastic cord pulls the guide block and rises this moment, thereby it goes up to take in to bottom deposit pole to drive the magnetic tube, the magnetic bead gets into in bottom deposit pole under magnetic adsorption, after transferring to corresponding position, downwards press the second clamp plate, descend through the second clamp plate and promote down the depression bar downwards, make the depression bar get into in bottom deposit pole after the depression bar descends, thereby push out the magnetic bead from bottom deposit pole through thrust and accomplish the transfer.

3. This magnetic bead transfer device for high flux nucleic acid extraction sets up through the cooperation of coupling mechanism and protection casing, and the in-process that uses is through inside push rod that promotes, drives spacing fixture block to a side motion through the connecting rod when extruding coupling spring in the interior push rod motion process for spacing fixture block withdraws from in the recess of spacing tank bottom side, can take off the protection casing this moment, can avoid the magnetic bead to drop at the transfer in-process.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a front sectional view of the present invention;

FIG. 3 is an enlarged schematic view of the structure at the position A of the present invention;

FIG. 4 is a schematic sectional view of the transfer tube of the present invention;

fig. 5 is a side view of the connection mechanism of the present invention.

In the figure: 1. a bottom mounting plate; 2. a connecting seat; 3. connecting a threaded hole; 4. transferring the tube; 401. a bottom storage pole; 402. a magnetic tube; 403. a lower pressure lever; 404. a guide groove; 405. a guide block; 406. an elastic cord; 407. a lower push rod; 408. a support spring; 5. a support bar; 6. a first presser plate; 7. a second platen; 8. a return spring; 9. a baffle plate; 10. a compression spring; 11. a connecting mechanism; 1101. connecting the mounting groove; 1102. an inner push rod; 1103. a connecting spring; 1104. a connecting rod; 1105. a limiting clamping block; 12. a protective cover; 1201. a limiting groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Example 1

Referring to fig. 1 to 3, the present invention provides a technical solution: magnetic bead transfer device for high flux nucleic acid extraction, including bottom mounting panel 1, the lower fixed surface of bottom mounting panel 1 is connected with connecting seat 2, be equipped with a plurality of threaded connection hole 3 on connecting seat 2, the quantity of threaded connection hole 3 is a plurality of, and the position of a plurality of threaded connection hole 3 corresponds different orifice plates respectively, a plurality of threaded connection hole 3 female connection has transfer pipe 4, transfer pipe 4 includes bottom deposit pole 401, magnetic tube 402 has been pegged graft at the top of bottom deposit pole 401, peg graft at the top of magnetic tube 402 has depression bar 403, bottom mounting panel 1 butt is run through at the lower surface of first clamp plate 6 at the top of magnetic tube 402, depression bar 403 runs through first clamp plate 6 butt at the lower surface of second clamp plate 7, be equipped with guide way 404 on the lateral wall of bottom deposit pole 401 internal surface, the outer surface fixed surface of magnetic tube 402 is connected with guide block 405, guide block 405 sliding connection is in guide way 404, and the top fixed connection of guide way has elastic rope 406, the other end fixed connection of elastic rope 406 is on the upper surface 407 of guide block 405, the lower surface 407 of depression bar 403 is connected with depression bar 407, the lower surface of support spring 408 that supports the depression bar 408 that supports.

In the use, through the cooperation setting of bottom mounting panel 1, connecting seat 2, threaded connection hole 3 and transfer pipe 4, can be according to the interval in each hole on the orifice plate that uses in the corresponding threaded connection hole 3 of transfer pipe 4 setting, enlarged the application range of this device.

Example 2

Referring to fig. 1, 4 and 5, the present invention provides a technical solution: the magnetic bead transfer device for high-flux nucleic acid extraction comprises a bottom mounting plate 1, wherein a supporting rod 5 is fixedly connected to the upper surface of the bottom mounting plate 1, a first pressing plate 6 is slidably connected to the middle of the supporting rod 5, a second pressing plate 7 is slidably connected to the top of the supporting rod 5, a transfer tube 4 comprises a bottom storage rod 401, a magnetic tube 402 is inserted into the top of the bottom storage rod 401, a lower pressing rod 403 is inserted into the top of the magnetic tube 402, the top of the magnetic tube 402 penetrates through the bottom mounting plate 1 and abuts against the lower surface of the first pressing plate 6, the lower pressing rod 403 penetrates through the first pressing plate 6 and abuts against the lower surface of the second pressing plate 7, a reset spring 8 is sleeved at the bottom of the supporting rod 5, the top of the reset spring 8 abuts against the lower surface of the first pressing plate 6, a baffle 9 is fixedly connected to the outer surface of the supporting rod 5, and a compression spring 10 is sleeved at the top of the supporting rod 5, the position of baffle 9 is located the top of first clamp plate 6, compression spring 10's bottom butt is at the upper surface of baffle 9, compression spring 10's top butt is at the lower surface of second clamp plate 7, the both ends of bottom mounting panel 1 upper surface are equipped with coupling mechanism 11, coupling mechanism 11 includes connection mounting groove 1101, the both sides of connection mounting groove 1101 are pegged graft and are had interior push rod 1102, be equipped with coupling spring 1103 between two interior push rods 1102, the lower fixed surface of interior push rod 1102 is connected with connecting rod 1104, the spacing fixture block 1105 of bottom fixedly connected with of connecting rod 1104, the lower surface of bottom mounting panel 1 is equipped with protection casing 12, the top of protection casing 12 is equipped with spacing groove 1201, bottom mounting panel 1 is pegged graft at spacing groove 1201 to the one end of connecting rod 1104, and the cross-sectional shape of spacing groove 1201 is the T type.

In use, when a magnetic bead needs to be placed into the transfer tube 4 in the use process, the first pressing plate 6 is pressed downwards to drive each magnetic tube 402 to descend, the bottom of the magnetic tube extends out of the lower portion of the bottom storage rod 401, the magnetic bead is adsorbed onto the magnetic tube 402, the first pressing plate 6 is loosened, the first pressing plate 6 is pushed to reset by the reset spring 8, the elastic rope 406 pulls the guide block 405 to ascend, the magnetic tube 402 is driven to ascend and enter the bottom storage rod 401, the magnetic bead enters the bottom storage rod 401 under magnetic adsorption, after the magnetic bead is transferred to a corresponding position, the second pressing plate 7 is pressed downwards, the second pressing plate 7 descends to push the lower pressing rod 403 downwards, the lower pressing rod 403 descends to enable the lower pressing rod 407 to enter the bottom storage rod 401, the magnetic bead is pushed out of the bottom storage rod 401 through thrust to complete transfer, the inner pressing rod is pushed inwards, the connecting rod 1201 presses the connecting spring and drives the limiting fixture block 1104 to move to one side in the limiting groove 1105, the limiting fixture block 1103 can be removed from the limiting groove 1102, and the protective cover 1103 can be prevented from dropping in the lower pressing rod 1102.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A magnetic bead transfer device for high-flux nucleic acid extraction comprises a bottom mounting plate (1), and is characterized in that: the lower fixed surface of bottom mounting panel (1) is connected with connecting seat (2), be equipped with a plurality of threaded connection hole (3) on connecting seat (2), it is a plurality of threaded connection hole (3) female connection has transfer pipe (4), the last fixed surface of bottom mounting panel (1) is connected with bracing piece (5), the middle part sliding connection of bracing piece (5) has first clamp plate (6), the top sliding connection of bracing piece (5) has second clamp plate (7), reset spring (8) have been cup jointed to the bottom of bracing piece (5), the top butt of reset spring (8) is at the lower surface of first clamp plate (6), the external fixed surface of bracing piece (5) is connected with baffle (9), compression spring (10) have been cup jointed at the top of bracing piece (5), the both ends of bottom mounting panel (1) upper surface are equipped with coupling mechanism (11), the lower surface of bottom mounting panel (1) is equipped with protection casing (12).

2. The magnetic bead transfer device for high-throughput nucleic acid extraction according to claim 1, wherein: the number of the threaded connecting holes (3) is a plurality of, and the positions of the threaded connecting holes (3) correspond to different pore plates respectively.

3. The magnetic bead transfer device for high-throughput nucleic acid extraction according to claim 1, wherein: the transfer pipe (4) comprises a bottom storage rod (401), a magnetic pipe (402) is inserted into the top of the bottom storage rod (401), a lower pressing rod (403) is inserted into the top of the magnetic pipe (402), the top of the magnetic pipe (402) penetrates through the bottom mounting plate (1) and abuts against the lower surface of the first pressing plate (6), and the lower pressing rod (403) penetrates through the first pressing plate (6) and abuts against the lower surface of the second pressing plate (7).

4. A magnetic bead transfer device for high throughput nucleic acid extraction according to claim 3, wherein: be equipped with guide way (404) on the lateral wall of bottom stock pole (401) internal surface, the outer fixed surface of magnetic tube (402) is connected with guide block (405), guide block (405) sliding connection is in guide way (404), just the top fixed connection of guide way (404) has elastic rope (406), the other end fixed connection of elastic rope (406) is at the upper surface of guide block (405).

5. The magnetic bead transfer device for high-throughput nucleic acid extraction according to claim 3, wherein: the lower surface of the lower pressing rod (403) is fixedly connected with a lower pushing rod (407), the outer surface of the lower pushing rod (407) is sleeved with a supporting spring (408), one end of the supporting spring (408) is abutted to the top of the lower pressing rod (403), and the lower pushing rod (407) penetrates through the magnetic pipe (402).

6. A magnetic bead transfer device for high throughput nucleic acid extraction according to claim 1, wherein: the position of the baffle plate (9) is located above the first pressing plate (6), the bottom of the compression spring (10) abuts against the upper surface of the baffle plate (9), and the top of the compression spring (10) abuts against the lower surface of the second pressing plate (7).

7. The magnetic bead transfer device for high-throughput nucleic acid extraction according to claim 1, wherein: the connecting mechanism (11) comprises a connecting installation groove (1101), inner push rods (1102) are inserted into two sides of the connecting installation groove (1101), a connecting spring (1103) is arranged between the inner push rods (1102), a connecting rod (1104) is fixedly connected to the lower surface of each inner push rod (1102), and a limiting clamping block (1105) is fixedly connected to the bottom of each connecting rod (1104).

8. A magnetic bead transfer device for high throughput nucleic acid extraction according to claim 7, wherein: the top of the protective cover (12) is provided with a limiting groove (1201), one end of the connecting rod (1104) penetrates through the bottom mounting plate (1) and is inserted into the limiting groove (1201), and the cross section of the limiting groove (1201) is T-shaped.