CN218046591U - Medium supply device of purification system - Google Patents
Medium supply device of purification system Download PDFInfo
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- CN218046591U CN218046591U CN202220502855.4U CN202220502855U CN218046591U CN 218046591 U CN218046591 U CN 218046591U CN 202220502855 U CN202220502855 U CN 202220502855U CN 218046591 U CN218046591 U CN 218046591U
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Abstract
The utility model relates to the field of laboratory purification instruments, and discloses a medium supply device of a purification system, which comprises a mounting rack, and a sample container, a chromatography unit and a balance pipe which are sequentially arranged on the mounting rack from top to bottom; the sample container is used for containing a stock solution sample, and the liquid outlet end of the sample container is provided with a control valve for controlling the stock solution sample to flow out; the liquid inlet end and the liquid outlet end of the chromatography unit are respectively connected with the liquid outlet end of the sample container and the liquid inlet end of the balance pipe and are used for separating a required medium in a stock solution sample; the balance tube is U-shaped, and the liquid outlet end of the balance tube is not lower than the liquid inlet end of the chromatography unit, so that the pressure of the liquid inlet end of the chromatography unit and the pressure of the liquid outlet end of the balance tube are balanced, and air is prevented from entering the chromatography unit. The utility model provides a purification system's medium feeding device can prevent the air admission chromatography unit.
Description
Technical Field
The utility model relates to a laboratory purification apparatus field, concretely relates to medium feeding device of purification system.
Background
The antibody is a special protein molecule, is used as a reagent for in vitro diagnosis, a medicament for treating diseases, a ligand for immunoaffinity chromatography and the like, and has wide application in the fields of life science research, biotechnology and medicine. The preparation of antibodies requires the separation and purification of biological raw materials such as serum or animal cell culture supernatant, and at present, the conventional purification method in laboratories is to pump a stock solution sample in a container by a peristaltic pump and pump the sample into a chromatographic column, and then separate specific antibodies aiming at target proteins from a plurality of antibody mixed samples by the chromatographic column.
However, the pipeline of the peristaltic pump contains air, which causes bubbles to be generated in the stock solution sample in the pipeline, and the pumping of the stock solution sample containing bubbles into the chromatographic column causes the flow rate of the stock solution sample passing through the filler in the chromatographic column to be reduced, and the problem of uneven filler is solved, so that the chromatographic speed is greatly reduced, and the separation and purification effects are influenced.
In summary, it is necessary to design a medium supply device of a purification system to solve the above problems.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model provides a medium feeding device of purification system can prevent the air admission chromatography unit.
(II) technical scheme
In order to solve the technical problem, the utility model provides a following technical scheme: a medium supply device of a purification system comprises a mounting frame, and a sample container, a chromatography unit and a balance pipe which are arranged on the mounting frame from top to bottom in sequence;
the sample container is used for accommodating a stock solution sample, and the liquid outlet end of the sample container is provided with a control valve for controlling the stock solution sample to flow out;
the liquid inlet end and the liquid outlet end of the chromatography unit are respectively connected with the liquid outlet end of the sample container and the liquid inlet end of the balance pipe and are used for separating a required medium in a stock solution sample;
the balance tube is U-shaped, and the liquid outlet end of the balance tube is not lower than the liquid inlet end of the chromatography unit, so that the pressure of the liquid inlet end of the chromatography unit and the pressure of the liquid outlet end of the balance tube are balanced, and air is prevented from entering the chromatography unit.
Further, an exhaust part is movably arranged in the sample container; the air exhaust member is provided with an air exhaust channel communicated with the interior of the sample container, closes the sample container and is relatively displaced along the axial direction of the sample container so as to exhaust air in the sample container through the air exhaust channel.
So set up, when the control valve was closed, the bottom displacement of air discharge spare pressurized orientation sample container can form the negative pressure in sample container, follow exhaust passage internal discharge with sample container and the air of adorning in the stoste sample in the sample container together, avoid the chromatography unit to advance the bubble, reduce chromatography speed to influence separation and purification's effect.
Further provided, the exhaust member comprises a piston portion and a push rod portion; the piston part is in interference fit with the interior of the sample container; the bottom end of the push rod part is fixedly connected with the piston part, and the top end of the push rod part extends out of the sample container.
So set up, the sample container is sealed through the piston portion with the inside interference fit of sample container to the air discharge spare, exerts pressure through the top to the push rod portion again to the bottom displacement of linkage piston portion towards sample container, thereby can form the negative pressure in sample container.
The axial line of the piston part is provided with a tapered hole; the axis of the push rod part is provided with a connecting hole corresponding to the conical hole, and the conical hole and the connecting hole form an exhaust passage.
So set up, the bell mouth can improve exhaust efficiency, passes through exhaust passage exhaust with the air in sample container and the stoste sample fast.
The top end of the push rod part is rotatably connected with a blocking piece for blocking an exhaust channel; the opening end of the connecting hole, which is far away from the piston part, is provided with a conical concave part; the bottom of the blocking piece is provided with an elastic piece in interference fit with the conical concave part.
So set up, when the elastic component of stifled piece rotation to its bottom was counterpointed the cooperation with the toper concave part, the exhaust passage was kept away from the one end of piston portion and is blocked by the stifled piece, otherwise, when the elastic component staggers with the toper concave part, the exhaust passage was opened.
And a rotating handle part is arranged at the top of the plug part and is used for controlling the elastic part to be aligned or staggered with the conical concave part.
So set up, make things convenient for researcher application of force to rotate stifled piece to control elastic component and toper concave part counterpoint or stagger, thereby control stifled piece shutoff or open exhaust passage.
Further, a vibration component is arranged between the sample container and the mounting frame and is used for vibrating the stock solution sample in the sample container.
So set up, the vibration part can replace the stoste sample in artifical quick shock sample container, effectively discharges the air from the stoste sample in, avoids the chromatography unit to advance the bubble, reduces chromatography speed.
The medium supply device further comprises a filter unit arranged on the mounting frame; the filtering unit is respectively connected with the liquid outlet end of the sample container and the liquid inlet end of the chromatography unit and is used for filtering impurities in the stock solution sample.
So set up, can filter the impurity in the stoste sample through the filter unit to block up the chromatography unit, reduce purification efficiency.
The medium supply device further comprises a liquid storage unit arranged on the mounting frame; the liquid storage unit is connected with the liquid outlet end of the balance tube and is used for storing media after chromatography.
So set up, through the medium after the stock solution unit storage chromatography, provide required medium for the purification system.
(III) advantageous effects
Compared with the prior art, the utility model provides a pair of medium feeding device of purification system possesses following beneficial effect:
when separating and purifying, firstly, extracting a stock solution sample into a sample container; and opening the control valve to control the stock solution sample to flow out of the sample container, automatically flowing the stock solution sample into the chromatography unit through self gravity for chromatography, and allowing the medium after chromatography to flow out of the chromatography unit through the balance pipe to provide a required medium for a purification system.
Therefore, the medium supply device provided by the utility model balances the pressure at the liquid inlet end of the chromatographic unit and the pressure at the liquid outlet end of the balance pipe through the balance pipe, can effectively prevent the air in the stock solution sample from entering the chromatographic unit, solves the problems that the stock solution sample containing air bubbles is pumped into the chromatographic column to cause the flow velocity of the stock solution sample to be reduced through the filler in the chromatographic column and the stock solution sample containing air bubbles is uneven through the filler, further improves the chromatographic speed and ensures the separation and purification effects; and the stock solution sample automatically flows into the chromatography unit through the self gravity, so that the use of a peristaltic pump for pumping the stock solution sample into the chromatography unit is omitted, and the problems that the air is contained in a pipeline of the peristaltic pump, so that bubbles are generated in the stock solution sample, and the chromatography speed is reduced are solved.
Drawings
Fig. 1 is a schematic perspective view of a medium supply device of a purification system according to the present invention;
fig. 2 is a sectional view of the medium supplying device of the purifying system according to the present invention.
Reference numbers: 1. a mounting frame; 11. hanging plates; 12. a base plate; 2. a sample container; 21. an exhaust member; 211. an exhaust passage; 212. a piston portion; 2121. a tapered hole; 213. a push rod part; 2131. connecting holes; 2132. a tapered recess; 214. plugging pieces; 2141. an elastic member; 2142. rotating the handle; 22. a control valve; 3. a filtration unit; 31. cotton; 4. a chromatography unit; 41. a chromatography column; 5. a balance tube; 6. a liquid storage unit; 7. a vibrating member; 8. a connecting portion; 81. a limit convex part.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1-2, a medium supply device of a purification system can provide a required medium for the purification system, discharge bubbles in a stock solution sample, improve chromatography efficiency, and solve the problem of dripping during purification.
The medium supply device comprises an installation rack 1, and a sample container 2, a filtering unit 3, a chromatography unit 4, a balance pipe 5 and a liquid storage unit 6 which are sequentially installed on the installation rack 1 from top to bottom, wherein the sample container 2, the filtering unit 3, the chromatography unit 4, the balance pipe 5 and the liquid storage unit 6 are sequentially connected.
The sample container 2 is used for containing a stock solution sample, and a control valve 22 for controlling the stock solution sample to flow out is arranged at the liquid outlet end of the sample container;
an exhaust member 21 is movably arranged in the sample container 2; the air discharging member 21 is provided with an air discharging passage 211 communicating with the inside of the sample container 2, and it closes the sample container 2 and relatively displaces in the axial direction of the sample container 2 to form a negative pressure in the sample container 2 and discharge the air in the sample container 2 through the air discharging passage 211.
The air discharge member 21 includes a piston portion 212 and a push rod portion 213; the piston portion 212 is an interference fit with the interior of the sample container 2 to close the sample container 2; the bottom end of the push rod part 213 is fixedly connected with the piston part 212, and the top end of the push rod part 213 extends out of the sample container 2, so that a researcher can control the piston part 212 to move up and down in the sample container 2 through the top end of the push rod part 213.
A tapered hole 2121 is formed on the axis of the piston part 212; the axis of the push rod 213 is opened with a connection hole 2131 corresponding to the taper hole 2121, and the taper hole 2121 and the connection hole 2131 form an exhaust passage 211, and the taper hole 2121 can improve the exhaust efficiency and exhaust the air in the sample container 2 and the raw liquid sample through the exhaust passage 211 quickly.
The top end of the push rod part 213 is rotatably connected with a blocking piece 214 for blocking the exhaust channel 211; the opening end of the connecting hole 2131 far away from the piston part 212 is provided with a conical concave part 2132; the bottom of the plug 214 is provided with an elastic member 2141 which is in interference fit with the tapered recess 2132, and the top of the plug 214 is provided with a rotating handle 2142, and the rotating handle 2142 is used for controlling the alignment or the staggering of the elastic member 2141 and the tapered recess 2132, so that the plug 214 is controlled to plug or open the exhaust passage 211.
The elastic member 2141 is made of rubber.
Install vibrating part 7 between sample container 2 and the mounting bracket 1, vibrating part 7 can replace artifical quick shock sample container 2 interior stoste sample, effectively discharges the air from the stoste sample in, avoids chromatography unit 4 to advance the bubble, reduces chromatography speed.
The filtering unit 3 is filled with cotton 31 to filter impurities in the raw liquid sample, so as to prevent the chromatographic column 41 from being blocked and reduce the purification efficiency.
The chromatography unit 4 employs a chromatography column 41, and the chromatography column 41 is used to separate a desired medium in a raw liquid sample.
The balance pipe 5 is U-shaped, and the liquid outlet end of the balance pipe is not lower than the liquid inlet end of the chromatography unit 4, so that the pressure of the liquid inlet end of the chromatography unit 4 and the pressure of the liquid outlet end of the balance pipe 5 are balanced, and air is prevented from entering the chromatography unit 4.
The liquid storage unit 6 adopts a container such as a beaker and the like, and is used for storing the medium after chromatography and providing the required medium for the purification system.
Be fixed with two-layer link plate 11 on the mounting bracket 1, have the difference in height between two-layer link plate 11, all be located the filter unit 3 below, wherein, the play liquid end of chromatography column 41 and balance tube 5 all installs on the link plate 11 that is located the top, and the feed liquor end of balance tube 5 is installed on the link plate 11 that is located the below.
Connecting parts 8 are respectively installed at the liquid inlet end of the chromatographic column 41 and the liquid inlet end and the liquid outlet end of the balance pipe 5, a limiting convex part 81 is arranged on the outer side of each connecting part 8, so that the chromatographic column 41 and the balance pipe 5 are hung on the hanging plate 11, and the installation mode is simple and convenient.
In the following description, as shown in fig. 1-2, the present invention is exemplified by a medium supplying apparatus of a purifying system, which mainly comprises: mounting bracket 1, sample container 2, filter unit 3, chromatography unit 4, balance tube 5 and stock solution unit 6.
During actual assembly and use: (1) The sample container 2 is hung on the mounting rack 1, and the liquid outlet end at the bottom of the sample container is provided with a control valve 22;
the piston part 212 of the air vent piece 21 is in interference fit with the interior of the sample container 2, and the material is rubber; the bottom end of the push rod part 213 is bonded with the piston part 212, and the top end extends out of the sample container 2; the top end of the push rod part 213 is hinged with a blocking piece 214 for blocking the exhaust channel 211, and the material of the blocking piece 214 is a metal plate; the bottom of the plug 214 is adhered with an elastic member 2141, and the top is integrally connected with a rotating handle 2142, the rotating handle 2142 is positioned right above the elastic member 2141; the elastic part 2141 is made of silicon gel;
(2) The liquid inlet end at the top of the filtering unit 3 is connected with the liquid outlet end at the bottom of the sample container 2 through a hose, and the liquid outlet end at the bottom of the filtering unit is connected with the liquid inlet end of the chromatographic column 41 through a hose; the inside of the filter unit 3 is filled with cotton 31;
(3) Two layers of hanging plates 11 are welded on the mounting rack 1, a vibration part 7 is also mounted on the mounting rack 1, and the vibration part 7 adopts the existing vibrator; and the vibration member 7 is positioned between the sample container 2 and the mounting frame 1 and is in close contact with the sample container 2; a bottom plate 12 is welded at the bottom of the mounting frame 1, so that the overall stability of the medium supply device is improved, and collapse is avoided;
(4) The liquid inlet end of the chromatographic column 41 and the liquid inlet and outlet ends of the balance pipe 5 are both connected with a connecting part 8 in a bonding or threaded manner, the outer side of the connecting part 8 is integrally connected with a limiting convex part 81, and the connecting part 8 penetrates through the hanging plate 11 and is hung and placed on the hanging plate through the limiting convex part 81; the chromatographic column 41 is suspended on the hanging plate 11 positioned above through the connecting part 8, the liquid inlet end of the balance pipe 5 is suspended on the hanging plate 11 positioned below through the connecting part 8, and the liquid outlet end at the bottom end of the chromatographic column 41 is connected with the connecting part 8 at the liquid inlet end of the balance pipe 5, so that the chromatographic column 41 is communicated with the balance pipe 5; the liquid outlet end of the balance pipe 5 sequentially penetrates through the two hanging plates 11 and is hung on the hanging plate 11 positioned above through the connecting part 8;
(6) The connecting part 8 at the liquid outlet end of the balance pipe 5 is connected with a hose, and the liquid outlet end of the hose extends into the liquid storage unit 6, so that the balance pipe 5 is communicated with the liquid storage unit 6.
The working principle in actual use is as follows:
when the medium supply device of the purification system is used for separation and purification, firstly, the stock solution sample is extracted into the sample container 2, then the vibration part 7 is started to vibrate the stock solution sample in the sample container 2, and bubbles in the stock solution sample are discharged to the upper part of the sample container 2; simultaneously, the bottom end of the air discharging member 21 is inserted into the specimen container 2, the air discharging member 21 is manually pressed and moved downwards to form negative pressure in the specimen container 2, and air in the specimen container 2 is discharged from the air discharging channel 211 until a part of the stock solution sample is subsequently discharged from the air discharging channel 211, which indicates that the air in the specimen container 2 is completely discharged; after emptying, the plug 214 is rotated by rotating the handle 2142, so that the elastic piece 2141 at the bottom of the plug is aligned and matched with the conical concave part 2132, and the exhaust channel 211 is plugged; then the control valve 22 is opened, and the stock solution sample in the sample container 2 automatically flows into the filtering unit 3 for filtering through self gravity; after filtration, the stock solution sample automatically flows into the chromatographic column 41 through self gravity for chromatography, the medium after chromatography flows into the balance pipe 5, the pressure of the liquid inlet end of the chromatography unit 4 and the pressure of the liquid outlet end of the balance pipe 5 are balanced by the balance pipe 5, and air in the stock solution sample can be effectively prevented from entering the chromatography unit 4; the medium after chromatography flows into a liquid storage unit 6 through an equilibrium pipe 5 to provide the required medium for a purification system.
The utility model provides a medium feeding device of purification system balances the pressure that flows out the liquid end through balance pipe 5 to chromatography unit 4 feed liquor end and balance pipe 5, can effectively prevent the air in the stoste sample to get into chromatography unit 4, still discharge the air in sample container 2 and the stoste sample through air discharge 21, under air discharge 21 and the dual function of balance pipe 5 cooperation, chromatography column 41 air inlet bubble has effectively been avoided, the problem of the stoste sample pump that contains the bubble is gone into in chromatography column 41 and is leaded to its velocity of flow reduction through the filler in chromatography column 41 is solved, and through the inhomogeneous problem of filler, and then improved the chromatography speed, guarantee the effect of separation and purification; the stock solution sample automatically flows into the filtering unit 3, the chromatography unit 4, the balance pipe 5 and the liquid storage unit 6 from the sample container 2 in sequence through the self gravity, so that the use of a peristaltic pump for pumping the stock solution sample into the chromatography unit 4 is omitted, and the problems that the air is contained in a pipeline of the peristaltic pump, so that bubbles are generated in the stock solution sample, and the chromatography speed is reduced are solved; and the chromatography is carried out after the filtration by the filter unit 3, so that the chromatographic column 41 is effectively prevented from being blocked by impurities.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The medium supply device of the purification system is characterized by comprising a mounting frame, and a sample container, a chromatography unit and a balance pipe which are arranged on the mounting frame in sequence from top to bottom;
the sample container is used for accommodating a stock solution sample, and the liquid outlet end of the sample container is provided with a control valve for controlling the stock solution sample to flow out;
the liquid inlet end and the liquid outlet end of the chromatography unit are respectively connected with the liquid outlet end of the sample container and the liquid inlet end of the balance pipe and are used for separating a required medium in a stock solution sample;
the balance pipe is U-shaped, and the liquid outlet end of the balance pipe is not lower than the liquid inlet end of the chromatography unit, so that the pressure of the liquid inlet end of the chromatography unit and the pressure of the liquid outlet end of the balance pipe are balanced, and air is prevented from entering the chromatography unit.
2. The media supply of claim 1, wherein the sample container is movably provided with a vent inside; the exhaust piece is provided with an exhaust passage communicated with the interior of the sample container, closes the sample container and relatively displaces along the axial direction of the sample container so as to exhaust air in the sample container through the exhaust passage.
3. The media supply of a purification system as claimed in claim 2, wherein said vent member comprises a piston portion and a push rod portion; the piston part is in interference fit with the interior of the sample container; the bottom end of the push rod part is fixedly connected with the piston part, and the top end of the push rod part extends out of the sample container.
4. The media supply of claim 3, wherein the piston portion has a tapered bore formed along an axis thereof; and a connecting hole is formed in the axis of the push rod part corresponding to the conical hole, and the conical hole and the connecting hole form an exhaust passage.
5. The medium supplying device of a purifying system according to claim 4, wherein a plugging member for plugging the air discharging passage is rotatably connected to the top end of the push rod part; the opening end of the connecting hole, which is far away from the piston part, is provided with a conical concave part; the bottom of the blocking piece is provided with an elastic piece in interference fit with the conical concave part.
6. The media supply of claim 5, wherein the top of the plug is provided with a rotating handle for controlling the alignment or misalignment of the resilient member with the tapered recess.
7. The media supply of any one of claims 1 to 6, wherein a vibration member is disposed between the sample container and the mounting frame for oscillating the sample solution in the sample container.
8. The media supply of a purification system according to any one of claims 1 to 6, further comprising a filter unit disposed on the mounting frame; the filtration unit is connected with the liquid outlet end of the sample container and the liquid inlet end of the chromatography unit respectively, and is used for filtering impurities in a stock solution sample.
9. The media supply of a purification system according to any one of claims 1 to 6, further comprising a reservoir unit provided on the mounting frame; the liquid storage unit is connected with the liquid outlet end of the balance pipe and is used for storing media after chromatography.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220502855.4U CN218046591U (en) | 2022-03-08 | 2022-03-08 | Medium supply device of purification system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220502855.4U CN218046591U (en) | 2022-03-08 | 2022-03-08 | Medium supply device of purification system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218046591U true CN218046591U (en) | 2022-12-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220502855.4U Active CN218046591U (en) | 2022-03-08 | 2022-03-08 | Medium supply device of purification system |
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| Country | Link |
|---|---|
| CN (1) | CN218046591U (en) |
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- 2022-03-08 CN CN202220502855.4U patent/CN218046591U/en active Active
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