CN216877953U

CN216877953U - Novel pre-fabricated hollow column and column assembling system thereof

Info

Publication number: CN216877953U
Application number: CN202121181876.2U
Authority: CN
Inventors: 周胜; 徐慧; 左翔
Original assignee: Lisure Science Suzhou Co ltd
Current assignee: Lisure Science Suzhou Co ltd
Priority date: 2021-05-28
Filing date: 2021-05-28
Publication date: 2022-07-05
Anticipated expiration: 2031-05-28

Abstract

The utility model discloses a novel pre-assembled hollow column and a column assembling system thereof. The novel pre-fabricated hollow column comprises a vertical and hollow column body, wherein an upper end cover and a lower end cover are respectively arranged at the upper end and the lower end of the column body, a through first through hole is formed in the upper end cover, a through second through hole is formed in the lower end cover, and the second through hole is communicated with a vertical waste discharge pipeline; the side surface of the column body is communicated with a large cavity through a small cavity, and the large cavity is communicated with a filler inlet. The pre-filled hollow column can remove air bubbles generated in the column through the through hole in time by arranging the screw rod, the sealing gasket and the like at the inlet to control the filler, thereby reducing noise peaks, avoiding the reduction of analysis sensitivity, being directly used for chromatography work, not needing to increase preparation work and improving the work efficiency.

Description

Novel pre-fabricated hollow column and column assembling system thereof

Technical Field

The utility model relates to the field of biochemical equipment, in particular to a novel pre-installed hollow column and a column installing system thereof.

Background

The pre-packed column is an experimental consumable for adding various chromatographic fillers into the column and obtaining target protein by utilizing the characteristics of the fillers. Whereas filling by wet methods is generally also referred to as homogenization.

The structure of the pre-assembled column used at present comprises a column body, an upper flange cover, a lower flange cover, an upper inlet, a lower inlet and an upper outlet and a sieve plate. When the hollow column is loaded with the filler, the homogenate prepared from the filler and pure water enters from the top inlet and is filled on the sieve plate, the disadvantage is that the bubble can be generated when entering the hollow column due to the adoption of wet filling, the bubble can generate a noise peak, the analysis sensitivity can be reduced when the bubble is serious, the bubble in the column can be reduced only by a system pump of a chromatography system at present, but the bubble still exists in the column, meanwhile, the preparation work before chromatography is also increased, and the pre-packed column cannot be directly used for chromatography work.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model provides a novel pre-assembled hollow column and a column assembling system thereof.

According to one aspect of the utility model, the pre-fabricated hollow column comprises a vertical hollow column body, wherein an upper end cover and a lower end cover are respectively arranged at the upper end and the lower end of the column body, a first through hole is formed in the upper end cover in a penetrating mode, a second through hole is formed in the lower end cover in a penetrating mode, and the second through hole is communicated with a vertical waste discharge pipeline; the side surface of the column body is communicated with a large cavity through a small cavity, and the large cavity is communicated with a filler inlet.

According to the pre-packed hollow column and the column loading system thereof, the screw rod, the sealing gasket and the like are arranged at the inlet of the pre-packed hollow column to control the filler, so that bubbles generated in the column can be discharged through the through hole in time, the noise peak is reduced, the reduction of analysis sensitivity is avoided, the pre-packed hollow column can be directly used for chromatography, the preparation work does not need to be added, and the work efficiency is improved.

In some embodiments, the lower part of the large cavity is communicated with a filler inlet through a third through hole, wherein a screw rod is installed in the large cavity, and the tail end of the screw rod is provided with a sealing gasket. Thereby, the filling condition can be controlled by operating the rotating screw when filling from the filling inlet.

In some embodiments, a first seal ring is disposed between each of the upper and lower end caps and the column. Therefore, the upper end cover and the lower end cover of the pre-fabricated hollow column can be effectively sealed with the column body through the first sealing ring.

According to one aspect of the utility model, the column filling system applying the pre-filled hollow column comprises a homogenate tank, a pure water tank, a constant pressure conveying module, a middle column, the pre-filled hollow column and a main pipeline, wherein the homogenate tank and the pure water tank are both connected with the inlet of the main pipeline, the constant pressure conveying module is installed on the main pipeline, and the outlet of the main pipeline is connected with the pre-filled hollow column through the middle column.

In some embodiments, a first valve is arranged on the outlet pipeline of the homogenate tank, and a second valve is arranged on the outlet pipeline of the pure water tank. Therefore, the flow rates of the homogenate tank and the pure water tank can be respectively controlled through the first valve and the second valve.

In some embodiments, the constant pressure delivery module includes a diaphragm pump assembly and a pressure sensor both mounted on the main conduit. Thus, a specific composition of a constant pressure delivery module is described, the diaphragm pump assembly and pressure sensor being operable to control the delivery of liquid from the main conduit to ensure that it is at a constant pressure and speed.

In some embodiments, the middle column comprises a vertical hollow column body, an upper cover and a lower cover are respectively arranged at the upper end and the lower end of the hollow column body, a through bubble discharging port is arranged on the upper cover, and a through liquid inlet and a through liquid outlet are arranged on the lower cover; wherein the liquid inlet is connected with the outlet of the main pipeline, and the liquid outlet is connected with the filler inlet 0 of the pre-filled hollow column. Thereby, the main structure of the intermediate column is provided.

In some embodiments, the upper cover and the lower cover are both detachable, and a third sealing ring is arranged between the upper cover and the hollow cylinder. Therefore, the upper cover and the lower cover of the middle column and the hollow column can be effectively sealed through the third sealing ring.

In some embodiments, the hollow column body is provided with a vertical pipe, and the vertical pipe is connected to the lower cover and communicated with the liquid inlet. Thus, the liquid entering the hollow cylinder can be drained to a sufficient height through the vertical pipe.

In some embodiments, each of the conduits connected to the pre-packed column and the waste conduit is provided with a flow-stopping flow-controlling clip. Thus, the flow-stop flow-control clamp can control the flow of liquid into and out of the pre-packed hollow column.

Drawings

FIG. 1 is a schematic diagram of the construction of a novel pre-packed hollow column according to one embodiment of the present invention;

FIG. 2 is a schematic structural diagram of the area A shown in FIG. 1;

FIG. 3 is a schematic diagram of a column packing system employing the novel pre-packed hollow column of FIG. 1;

fig. 4 is a schematic structural view of the middle column shown in fig. 3.

In the figure: the device comprises a cylinder 1, an upper end cover 2, a clamping part 3, a first through hole 4, a second through hole 5, a waste discharge pipeline 6, a fixed support 7, a first support frame 8, a first sealing ring 9, a filler inlet 10, a large cavity 11, a third through hole 12, a screw 13, a sealing gasket 14, a second sealing ring 15, a small cavity 16, a lower end cover 17, a hollow cylinder 21, an upper cover 22, a lower cover 23, a foam discharge opening 24, a liquid inlet 25, a liquid outlet 26, a vertical pipe 27, a second support frame 28, a third sealing ring 29, a homogenizing tank 31, a pure water tank 32, a constant pressure conveying module 33, a first valve 34, a second valve 35, a middle column 36, a pre-installed hollow column 37, a flow stopping and controlling clamp 38 and a main pipeline 39.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Fig. 1 schematically shows the structure of a novel pre-packed hollow column according to an embodiment of the present invention, and fig. 2 shows the structure of the middle column of fig. 1. As shown in fig. 1-2, the pre-filled hollow column comprises a vertically arranged column body 1, the column body 1 is hollow, an upper end cover 2 and a lower end cover 17 are respectively arranged at the upper end and the lower end of the column body 1, and first sealing rings 9 are respectively arranged between the upper end cover 2 and the column body 1 and between the lower end cover 17 and the column body 1 for sealing.

The upper end cover 2 is provided with a through first through hole 4, the lower end cover 17 is provided with a through second through hole 5, and the first through hole 4 and the second through hole 5 are communicated with the inner cavity of the column body 1 and the outside. Wherein the first through holes 4 are used for removing air bubbles and the second through holes 5 are used for removing waste materials.

The second through hole 5 is communicated with a waste discharge pipeline 6, the waste discharge pipeline 6 is approximately vertical, and the upper end and the lower end of the waste discharge pipeline are respectively fixed on the column body 1 through two fixing brackets 7.

In addition, the bottom both sides of cylinder 1 still all are connected with first support frame 8 through block portion 3 to receive the stable support of first support frame 8.

The side surface of the cylinder 1 is communicated with a large cavity 11 through a small cavity 16, a screw 13 is arranged in the large cavity 11, the tail end of the screw is provided with a sealing gasket 14, and the screw 13 is provided with a second sealing ring 15 for sealing. Wherein, the lower part of the large cavity 11 is also communicated with a filler inlet 10 through a third through hole 12.

The filling of the filler can be controlled by operating the screw 13 to rotate, wherein the sealing gasket 14 can block the communication between the small cavity 16 and the large cavity 11 when the screw 13 rotates inward, thereby preventing foreign substances from entering the cylinder 1; when the screw 13 rotates outwards, the sealing gasket 14 is far away from the small cavity 16 to enable the large cavity 11 to be communicated, so that the filler can be introduced from the filler inlet 10 and enter the column body 1 through the third through hole 12, the large cavity 11 and the small cavity 16 in sequence, and air bubbles in the filler can be discharged through the first through hole 4 in the upper end cover 2.

Fig. 3 schematically shows the structure of a column packing system to which the novel pre-packed hollow column shown in fig. 1 is applied, and fig. 4 shows the structure of region a in fig. 3. As shown in fig. 3-4, the column packing system mainly comprises a homogenate tank 31, a pure water tank 32, a constant pressure feed module 33, an intermediate column 36, a pre-packed empty column 37 and a main pipe 39. The homogenizing tank 31 and the pure water tank 32 are connected to the inlet of the main pipe 39, the constant pressure delivery module 33 is installed on the main pipe 39, and the outlet of the main pipe 39 is connected to the pre-filled hollow column 37 through the intermediate column 36.

The outlet pipe of the homogenate tank 31 is provided with a first valve 34, and the outlet pipe of the pure water tank 32 is provided with a second valve 35, so that the flow rates of the liquid passing through the homogenate tank 31 and the pure water tank 32 can be controlled by the first valve 34 and the second valve 35, respectively. Wherein, the homogenate tank 31 is filled with filler, and the pure water tank 32 is filled with pure water, then the mixed liquid of the two is led into the main pipeline 39.

The constant pressure feeding module 33 includes a diaphragm pump assembly 331 and a pressure sensor 332, and the diaphragm pump assembly 331 and the pressure sensor 332 are mounted on the main pipe 39. Wherein, the pressure sensor 332 can monitor the pressure on the main pipeline 39 in real time and feed back the pressure information to the signal receiving end of the diaphragm pump assembly 332, so as to control the flow of the liquid through the diaphragm pump assembly 332 in time. The diaphragm pump assembly 332 then passes the liquid into the intermediate column 36.

The middle column 36 comprises a vertically arranged hollow column body 21, the hollow column body 21 is hollow, an upper cover 22 and a lower cover 23 which are all detachable are respectively arranged at the upper end and the lower end of the hollow column body, and third sealing rings 29 are respectively arranged between the upper cover 22 and the hollow column body 21 and between the lower cover 23 and the hollow column body 21 for sealing.

The upper cover 22 is provided with a through bubble discharging port 24, the lower cover 23 is provided with a through liquid inlet 25 and a through liquid outlet 26, and the bubble discharging port 24, the liquid inlet 25 and the liquid outlet 26 are all communicated with the inner cavity of the hollow column 21 and the outside. Wherein, inlet 25 is connected with the export of trunk line 39, and liquid can pass through inlet 25 from trunk line 39 and enter into intermediate column 36, and rethread liquid outlet 26 flows out, and the bubble in the liquid then can be discharged through bubble discharge port 24 simultaneously.

Further, a vertical pipe 27 is installed on the hollow cylinder 21, wherein the vertical pipe 27 is connected to the lower cover 23, and the vertical pipe 27 communicates with the liquid inlet 25. The vertical tube 27 can then, when the liquid is introduced, direct it to a sufficiently high position in the hollow cylinder 21 so that the gas bubbles can be sufficiently discharged through the bubble discharge opening 24.

Preferably, a second support bracket 28 is further provided at the bottom of the middle column 36 to stably support the middle column 36.

The outlet port 26 of the intermediate column 36 is connected to the filling inlet 10 of the pre-filled hollow column 37, and after the liquid exits the intermediate column 36 through the outlet port 26, the liquid can be introduced into the pre-filled hollow column 37 from the filling inlet 10, while the outlet port 26 can be connected to the pre-filled hollow column 37 by a hose.

Preferably, the conduits connected to the pre-filled hollow column 37, such as the hose connected to the liquid outlet 26, the external conduit connected to the first through hole 4, and the waste conduit 6 connected to the second through hole 5, can be provided with a flow-stopping clamp 38, so as to control the flow of liquid or gas therein.

In this embodiment, pre-packed hollow column 37 and intermediate column 36 may be made of stainless steel, glass, polymer, etc., which has biological and chemical compatibility with the sample and solution used in the process and sterilization process, such as resistance to 5M NaOH, 2M acetic acid, and conventional organic reagents such as benzyl alcohol, etc. The homogenization tank 31 and the pure water tank 32 are preferably containers capable of holding a filler and pure water.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the utility model.

Claims

1. The utility model provides a novel pre-installation hollow column which characterized in that: the waste heat recovery device comprises a vertical and hollow cylinder (1), wherein an upper end cover (2) and a lower end cover (17) are respectively arranged at the upper end and the lower end of the cylinder (1), a through first through hole (4) is formed in the upper end cover (2), a through second through hole (5) is formed in the lower end cover (17), and the second through hole (5) is communicated with a vertical waste discharge pipeline (6); the side of the column body (1) is communicated with a large cavity (11) through a small cavity (16), and the large cavity (11) is communicated with a filling material inlet (10).

2. A new pre-packed hollow column according to claim 1, wherein: the lower part of the large cavity (11) is communicated with a filler inlet (10) through a third through hole (12), wherein a screw rod (13) is installed in the large cavity (11), and a sealing gasket (14) is arranged at the tail end of the screw rod (13).

3. A new pre-packed hollow column according to claim 1, wherein: and a first sealing ring (9) is arranged between the upper end cover (2) and the lower end cover (17) and the column body (1).

4. A column packing system using a pre-packed hollow column according to any one of claims 1 to 3, wherein: the constant-pressure conveying device comprises a homogenizing tank (31), a pure water tank (32), a constant-pressure conveying module (33), a middle column (36), a pre-installed hollow column (37) and a main pipeline (39), wherein the homogenizing tank (31) and the pure water tank (32) are connected with an inlet of the main pipeline (39), the constant-pressure conveying module (33) is installed on the main pipeline (39), and an outlet of the main pipeline (39) is connected with the pre-installed hollow column (37) through the middle column (36).

5. The column packing system of claim 4, wherein: a first valve (34) is arranged on an outlet pipeline of the homogenate tank (31), and a second valve (35) is arranged on an outlet pipeline of the pure water tank (32).

6. The column packing system of claim 4, wherein: the constant pressure delivery module (33) includes a diaphragm pump assembly (331) and a pressure sensor (332) both mounted on the main pipe (39).

7. The column packing system of claim 4, wherein: the middle column (36) comprises a vertical hollow column body (21), an upper cover (22) and a lower cover (23) are respectively arranged at the upper end and the lower end of the hollow column body (21), a penetrating bubble discharging port (24) is formed in the upper cover (22), and a penetrating liquid inlet (25) and a penetrating liquid outlet (26) are formed in the lower cover (23); wherein the liquid inlet (25) is connected to the outlet of the main conduit (39) and the liquid outlet (26) is connected to the filling inlet (10) of the pre-filled hollow column (37).

8. The column packing system of claim 7, wherein: the upper cover (22) and the lower cover (23) are both detachable, and third sealing rings (29) are arranged between the upper cover and the hollow column body (21).

9. The column packing system of claim 7, wherein: a vertical pipe (27) is installed on the hollow column body (21), and the vertical pipe (27) is connected to the lower cover (23) and communicated with the liquid inlet (25).

10. The column packing system of claim 4, wherein: and each pipeline connected with the pre-installed hollow column (37) and the waste discharge pipeline (6) are provided with a flow stopping and controlling clamp (38).