CN209941009U

CN209941009U - Cell culture system

Info

Publication number: CN209941009U
Application number: CN201920155436.6U
Authority: CN
Inventors: 牟鳄贤
Original assignee: Sichuan Cancer Hospital
Current assignee: Sichuan Cancer Hospital
Priority date: 2019-01-29
Filing date: 2019-01-29
Publication date: 2020-01-14
Anticipated expiration: 2029-01-29

Abstract

The utility model relates to a cell culture system, including cell culture pipe, drive arrangement, be provided with the support that is used for loading the cell in the cell culture pipe, just the cell culture intussuseption is filled with the culture solution, the mouth of pipe of cell culture pipe is sealed through first glued membrane, drive arrangement is used for control first glued membrane is bloated from top to bottom. This system is through the upper and lower drumming of first glued membrane of control, leads to the culture solution to wash the cell on the support in the cell culture intraductal for the cell is more abundant to the absorption of nutrition, also can bring out the metabolic waste of cell, and the fluid shear mechanics effect that the culture solution that flows can also provide for the cell promotes its growth and breeds in addition.

Description

Cell culture system

Technical Field

The utility model relates to the field of medical equipment, concretely relates to cell culture system.

Background

At present, the conventional method for culturing cells is to statically culture target cells in a culture bottle or a culture dish, and the cells are usually proliferated and differentiated in a two-dimensional form after being attached to the wall. In recent years, various laboratories around the world have designed dynamic three-dimensional culture systems, aiming at solving the above problems, but still have the following disadvantages: 1. the lack of three-dimensional stereoregularity in the direction of flow of the culture medium results in a cell growth density inside the scaffold which is significantly lower than the surface of the scaffold, and a large amount of metabolic waste inside the scaffold gathers, which is not beneficial to cell growth.

SUMMERY OF THE UTILITY MODEL

The utility model aims to improve the defects existing in the prior art and provide a dynamic three-dimensional cell culture system.

The utility model provides a technical scheme that its technical problem adopted is:

the cell culture system comprises a cell culture tube and a driving device, wherein a support used for loading cells is arranged in the cell culture tube, a culture solution is filled in the cell culture tube, a tube opening of the cell culture tube is sealed through a first adhesive film, and the driving device is used for controlling the first adhesive film to vertically move.

The upper and lower of through the first glued membrane of drive arrangement control is bloated, leads to the culture solution to wash the cell on the support in the cell culture pipe for the cell is more abundant to the absorption of nutrition, also can bring out the metabolic waste of cell, and the fluid shear mechanics effect that the culture solution that flows can also provide for the cell promotes its growth and breeds moreover.

In a further scheme, the driving device comprises a control pump and a syringe, an injection port of the syringe is connected with a nozzle of the cell culture tube, and the control pump is used for controlling the relative displacement of a piston handle of the syringe and an empty cylinder.

In a further scheme, the driving device further comprises a first connecting pipe, one end of the first connecting pipe is connected with the injection port of the injector, and the other end of the first connecting pipe is connected with the pipe orifice of the cell culture pipe.

In a further aspect, the bottom of the cell culture tube is provided with a stabilizing member.

In a further embodiment, the number of the cell culture tubes is multiple, and the tube openings of the multiple cell culture tubes are all connected with the first connecting tube through a second connecting tube. And meanwhile, a plurality of cell culture tubes are controlled to culture cells, so that the cell culture efficiency is improved.

In a further scheme, the driving device further comprises a placing part, the inside of the placing part is hollow, the number of the cell culture tubes is multiple, a through hole matched with the tube opening of the cell culture tube is formed in the surface of the placing part and used for arranging and fixing the cell culture tubes, and an injection port of the injector is communicated with the placing part through a first connecting tube.

In a further aspect, the first connection tube and the syringe are both filled with a filling liquid or a filling gas. Preventing bacteria in the air from influencing the growth of cells.

In a further scheme, a branch pipe is arranged on the first connecting pipe, and a valve is arranged on the branch pipe. Filling liquid or filling gas is filled in the branch pipe and is closed through the valve, so that the tightness of the device is improved, and the influence of bacteria in the air on the growth of cells is prevented.

In a further scheme, a plurality of holes are formed in the first adhesive film to form a filter screen, the interior of the placement part is divided into an upper connection area and a lower connection area through the second adhesive film, the upper connection area is communicated with the through holes, the lower connection area is communicated with the first connection pipe, and culture solution is filled in the upper connection area. The flowing direction of the culture solution in the cell culture tube is diversified, and the cells on the bracket are washed in multiple directions.

In a further embodiment, the first adhesive film and the second adhesive film are both sterile adhesive films. Preventing bacteria from affecting the growth of cells.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. the upper and lower of through the first glued membrane of drive arrangement control is bloated, leads to the culture solution to wash the cell on the support in the cell culture pipe for the cell is more abundant to the absorption of nutrition, also can bring out the metabolic waste of cell, and the fluid shear mechanics effect that the culture solution that flows can also provide for the cell promotes its growth and breeds moreover.

2. The connecting pipe is connected with a plurality of groups of cell culture pipes, so that the cell culture efficiency is improved.

3. A plurality of holes are formed in the first adhesive film to form a filter screen, the interior of the placement part is divided into an upper connection area and a lower connection area through the second adhesive film, the upper connection area is communicated with the through holes, the lower connection area is communicated with the first connection pipe, and culture solution is filled in the upper connection area. The flowing direction of the culture solution in the cell culture tube is diversified, and the cells on the bracket are washed in multiple directions.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic diagram of the external structure of a cell culture system provided in example 1 of the present invention;

FIG. 2 is a front view of a cell culture system according to example 1 of the present invention;

FIG. 3 is a cross-sectional view of a cell culture tube of a cell culture system according to example 1 of the present invention;

FIG. 4 is a schematic diagram of the external structure of a cell culture system provided in example 2 of the present invention;

FIG. 5 is a schematic diagram of the external structure of a cell culture system provided in embodiment 3 of the present invention;

fig. 6 is a front view of a cell culture system according to embodiment 3 of the present invention.

Description of the drawings

Cell culture pipe 1, support 10, culture solution 11, first glued membrane 12, control pump 2, syringe 3, first connecting pipe 4, branch pipe 5, valve 6, second connecting pipe 7, place part 8, steadying 9, second glued membrane 13, go up joining region 14, lower joining region 15.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiment of the present invention, all other embodiments obtained by the person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

Referring to fig. 1-3, the present embodiment schematically discloses a cell culture system, including a cell culture tube 1 and a driving device, wherein a support 10 for loading cells is disposed in the cell culture tube 1, a culture solution 11 is filled in the cell culture tube 1, a tube opening of the cell culture tube is sealed by a first adhesive film 12, and the driving device is configured to control the first adhesive film 12 to move up and down.

In the scheme, the driving device comprises a control pump 2 and an injector 3, an injection port of the injector is connected with a pipe orifice of the cell culture pipe 1, and the control pump 2 is used for controlling the relative displacement of a piston handle of the injector 3 and an empty cylinder.

The suction of the injector 3 is controlled by the control pump 2, so that the up-and-down movement of the first rubber film 12 is controlled, as shown in fig. 3, the up-and-down movement of the first rubber film 12 causes the culture solution 11 to wash the cells on the bracket 10 in the cell culture tube 1, so that the cells can absorb nutrition more sufficiently, and can also bring out metabolic waste of the cells, and the flowing culture solution can also provide fluid shear mechanical action for the cells, thereby promoting the growth and the propagation of the cells.

In a further scheme, the driving device further comprises a first connecting pipe 4, one end of the first connecting pipe 4 is connected with an injection port of the injector 3, and the other end of the first connecting pipe 4 is connected with a pipe orifice of the cell culture pipe 1.

Meanwhile, the bottom of the cell culture tube 1 is provided with a stabilizing member 9. So that the cell culture tube 1 is more stably arranged. Under the condition of ensuring the stability of the cell culture tube, the structure or the shape and the size of the stable part are not limited.

And in order to prevent bacteria in the air from influencing the growth of cells, filling liquid is filled in the first connecting pipe and the syringe. Of course, the type of filling liquid is not limited to ensure that no bacteria can affect the growth of cells, and it is understood that other types of filling materials, such as filling gas, may be used. In this embodiment, distilled water is used as the filling liquid.

Furthermore, a branch pipe 5 is arranged on the first connecting pipe 4, and a valve 6 is arranged on the branch pipe 5. Filling liquid or filling gas is filled in through the branch pipe 5 and is closed through the valve 6, so that the convenience of filling the filling liquid or the filling gas is improved, meanwhile, the tightness of the device is also improved, and the influence of bacteria in the air on the growth of cells is prevented. Meanwhile, in this embodiment, the first adhesive film is an aseptic first adhesive film. Prevent the first glue film from having bacteria to influence the growth of cells.

In a preferred embodiment, the stent 10 is a ceramic stent. It will be readily appreciated that other three-dimensional scaffolds known in the art may be used, provided that cell growth is not affected.

In this embodiment, the cell culture tube 1 is a cell culture tube. The inner wall of the cell culture tube is smooth, so that the liquid adsorption residue can be reduced, and the tube wall of the cell culture tube is clear and easy to observe.

Example 2

Referring to fig. 4, the main difference between this embodiment 2 and the above embodiment 1 is that in this embodiment, the number of the cell culture tubes 1 is multiple, and the tube openings of the multiple cell culture tubes 1 are all connected to the first connecting tube 4 through the second connecting tube 7. And meanwhile, a plurality of cell culture tubes are controlled to culture cells, so that the cell culture efficiency is improved.

Example 3

Referring to fig. 5, the main difference between this embodiment 3 and the above embodiment 1 is that, in this embodiment, the driving device further includes a placing part 8, the inside of the placing part 8 is hollow, the number of the cell culture tubes 1 is multiple, a through hole adapted to the tube opening of the cell culture tube is formed on the surface of the placing part 8, the through hole is used for installing and fixing the cell culture tube 1, and the injection port of the injector 3 is communicated 8 with the placing part through a first connecting tube 4.

In a further scheme, as shown in fig. 6, a plurality of holes are formed in the first adhesive film 12 to form a filter screen, the interior of the placement part is divided into an upper connection area 14 and a lower connection area 15 through the second adhesive film 13, the upper connection area 14 is communicated with the through hole, the lower connection area 15 is communicated with the first connection pipe 4, and the upper connection area 14 is filled with a culture solution 11. The flowing direction of the culture solution 11 in the cell culture tube 1 is diversified, and the cells on the bracket 10 are washed in multiple directions.

In addition, in order to prevent bacteria from affecting the growth of cells, the first adhesive film 12 and the second adhesive film 13 are both sterile adhesive films.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The cell culture system is characterized by comprising a cell culture tube and a driving device, wherein a support used for loading cells is arranged in the cell culture tube, a culture solution is filled in the cell culture tube, a tube opening of the cell culture tube is sealed by a first adhesive film, and the driving device is used for controlling the first adhesive film to vertically move.

2. The cell culture system of claim 1, wherein the driving device comprises a control pump and a syringe, an injection port of the syringe is connected with a nozzle of the cell culture tube, and the control pump is used for controlling the relative displacement of a piston handle of the syringe and an empty cylinder.

3. The cell culture system according to claim 2, wherein the driving means further comprises a first connection tube having one end connected to the injection port of the syringe and the other end connected to the nozzle of the cell culture tube.

4. The cell culture system of claim 3, wherein the bottom of the cell culture tube is provided with a stabilizing member.

5. The cell culture system according to claim 3, wherein the number of the cell culture tubes is plural, and the nozzles of the plural cell culture tubes are connected to the first connecting tube through a second connecting tube.

6. The cell culture system according to claim 3, wherein the driving device further comprises a placement part, the placement part is hollow, the number of the cell culture tubes is multiple, a through hole matched with the tube openings of the cell culture tubes is formed in the surface of the placement part and used for arranging and fixing the cell culture tubes, and the injection port of the injector is communicated with the placement part through a first connecting tube.

7. The cell culture system according to claim 3, wherein the first connection tube and the syringe are both filled with a filling liquid or a filling gas.

8. The cell culture system according to claim 7, wherein a branch pipe is provided on the first connection pipe, and a valve is provided on the branch pipe.

9. The cell culture system according to claim 6, wherein the first adhesive film is provided with a plurality of holes to form a filter screen, the interior of the placement member is divided into an upper connection area and a lower connection area through a second adhesive film, the upper connection area is communicated with the through holes, the lower connection area is communicated with the first connection pipe, and the upper connection area is filled with a culture solution.

10. The cell culture system of claim 9, wherein the first adhesive film and the second adhesive film are both sterile adhesive films.