CN107158464A - A kind of automatic liquid supply formula takes off cell system - Google Patents
A kind of automatic liquid supply formula takes off cell system Download PDFInfo
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- CN107158464A CN107158464A CN201710397304.XA CN201710397304A CN107158464A CN 107158464 A CN107158464 A CN 107158464A CN 201710397304 A CN201710397304 A CN 201710397304A CN 107158464 A CN107158464 A CN 107158464A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3629—Intestinal tissue, e.g. small intestinal submucosa
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3625—Vascular tissue, e.g. heart valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
Cell system is taken off the invention discloses a kind of automatic liquid supply formula, is related to biological material deal device technical field.Invented to solve the problem of de- cell device of prior art can not adapt to production in enormous quantities and fluid infusion complex operation.Automatic liquid supply formula of the present invention takes off cell system, including：Shaking table, the shaking table includes swinging pallet；Cleaning case, the cleaning case is arranged on the swing pallet, and the cleaning case includes multiple rinse baths；Liquid-adding device, the liquid-adding device includes fluid reservoir, liquid-feeding tube and fluid filling pump, and described liquid-feeding tube one end is connected with the fluid reservoir, and the other end is stretched into the rinse bath, and the fluid filling pump is concatenated with the liquid-feeding tube；Liquid level gauge, the liquid level gauge is used to detect the liquid level in the rinse bath；Control unit, described control unit is connected with the fluid filling pump and the liquid level gauge respectively.The present invention can be used for biomaterial to take off cell.
Description
Technical field
Cell system is taken off the present invention relates to biological material deal device technical field, more particularly to a kind of automatic liquid supply formula.
Background technology
With the development of economy and society, the application of biomaterial medically also seems more and more prominent, biomaterial
Refer to the process of the material formed by biology, including cell and extracellular matrix.Conventional biomaterial have bovine pericardium and
Chitterlings, when biomaterial is applied into clinical, it is necessary to be cleaned to biomaterial, sterilizing, de- cell etc. are handled
Process.
In the prior art, de- cell is generally carried out using shaking table, specifically, a cleaning is fixed on the pallet of shaking table
Box, is put into biomaterial and de- cell reagent in cleaning box, is then turned on shaking table and is vibrated, in oscillatory process, cleaning
Box inwall is flopped repeatedly to biomaterial, so as to realize the de- cell of biomaterial.
But, because the oscillation amplitude of shaking table is limited, the volume of cleaning box can not be made big, can make if volume is too big
Cleaning box two lateral walls spacing it is very big, thus, biomaterial cleaning box vibrate during also not with cleaning box
Inwall produces slap, and just warp-wise opposite direction has been moved, so that de- cell can not be realized.Therefore, the de- cell cleaning of prior art
Box, in order to adapt to the oscillation amplitude of shaking table, usual volume is all smaller, it is impossible to adapt to produce in enormous quantities.Also, carrying out de- cell
During, de- cell reagent can produce foam, and amount of reagent can be gradually decreased, when biomaterial is more, the examination once added
Dosage possibly can not meet the thoroughly de- cell to biomaterial, need to mend when the amount of de- cell reagent is reduced to a certain extent
Liquid, prior art is to shut down shaking table in defensive position trend cleaning box to add reagent, and complex operation efficiency is low, and the amount of reagent added
Can not accurately it control.
The content of the invention
Embodiments of the invention provide a kind of automatic liquid supply formula and take off cell system, can increase the processing number of biomaterial
Amount, and can accurate fluid infusion automatically, raising operating efficiency.
To reach above-mentioned purpose, cell system is taken off The embodiment provides a kind of automatic liquid supply formula, including：Shake
Bed, the shaking table includes swinging pallet；Cleaning case, the cleaning case is arranged on the swing pallet, and the cleaning case includes
Multiple rinse baths, the rinse bath is used to place biomaterial and de- cell reagent；Liquid-adding device, the liquid-adding device includes storage
Flow container, liquid-feeding tube and fluid filling pump, described liquid-feeding tube one end are connected with the fluid reservoir, and the other end is stretched into the rinse bath, institute
Fluid filling pump is stated to concatenate with the liquid-feeding tube；Liquid level gauge, the liquid level gauge is used to detect the liquid level in the rinse bath；Control is single
Member, described control unit is connected with the fluid filling pump and the liquid level gauge respectively, when the liquid level that the liquid level gauge is detected is less than in advance
If during value, described control unit controls the fluid filling pump to open.
Automatic liquid supply formula of the present invention takes off cell system, because the cleaning case includes multiple rinse baths, therefore, each cleaning
The volume of groove can be made as adapting to the maximum volume of shaking table oscillation amplitude, compared with a cleaning box of prior art, increase
The processing quantity of biomaterial.And due to being provided with liquid-adding device and liquid level gauge, therefore, the liquid detected when the liquid level gauge
When position is less than preset value, control unit can control the fluid filling pump and open, and the de- cell reagent in fluid reservoir is pumped into rinse bath
It is interior, close fluid filling pump when reaching specified liquid level.Hereby it is achieved that automatic liquid supply, and can accurately control the examination of addition
Dosage, improves operating efficiency.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation that automatic liquid supply of embodiment of the present invention formula takes off cell system；
Fig. 2 is the internal structure schematic diagram that automatic liquid supply of embodiment of the present invention formula takes off cell system；
Fig. 3 is the structural representation for the interior layer box body that automatic liquid supply of embodiment of the present invention formula takes off cell system；
Fig. 4 is the polycrystalline substance schematic diagram for the interior layer box body that automatic liquid supply of embodiment of the present invention formula takes off cell system；
Fig. 5 is that the structure of another embodiment for the foam adsorption apparatus that automatic liquid supply of embodiment of the present invention formula takes off cell system is shown
It is intended to；
Fig. 6 is the structural representation for the internal layer case lid that automatic liquid supply of embodiment of the present invention formula takes off cell system.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
In the description of the invention, it is to be understood that term " " center ", " on ", " under ", "front", "rear", " left side ",
The orientation or position relationship of the instruction such as " right side ", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are based on shown in the drawings
Orientation or position relationship, are for only for ease of the description present invention and simplify description, rather than indicate or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In description of the invention, unless otherwise indicated, " multiple " are meant that two or more.
Reference picture 1, Fig. 2, the automatic liquid supply formula of the present embodiment take off cell system, including：Shaking table 3, the shaking table 3 includes pendulum
Dynamic pallet (not shown)；Cleaning case, the cleaning case is arranged on the swing pallet, and the cleaning case includes multiple clear
Washing trough 21, the rinse bath 21 is used to place biomaterial and de- cell reagent；Liquid-adding device 4, the liquid-adding device 4 includes storage
Flow container 41, liquid-feeding tube 42 and fluid filling pump 43, described one end of liquid-feeding tube 42 are connected with the fluid reservoir 41, and the other end stretches into described clear
In washing trough 21, the fluid filling pump 43 is concatenated with the liquid-feeding tube 42；Liquid level gauge (not shown), the liquid level gauge is used to detect
Liquid level in the rinse bath 21；Control unit (not shown), described control unit respectively with the fluid filling pump 43 and institute
Liquid level gauge connection is stated, when the liquid level that the liquid level gauge is detected is less than preset value, described control unit controls the fluid filling pump 43
Open.
Automatic liquid supply formula provided in an embodiment of the present invention takes off cell system, because the cleaning case includes multiple rinse baths
21, therefore, the volume of each rinse bath 21 can be made as adapting to the maximum volume of the oscillation amplitude of shaking table 3, with prior art
One cleaning box is compared, and increases the processing quantity of biomaterial.And due to being provided with liquid-adding device 4 and liquid level gauge, therefore,
When the liquid level that the liquid level gauge is detected is less than preset value, control unit can control the fluid filling pump 43 and open, by fluid reservoir 41
Interior de- cell reagent is pumped into rinse bath 21, and fluid filling pump 43 is closed when reaching specified liquid level.Hereby it is achieved that automatic
Fluid infusion, and the amount of reagent of addition can be accurately controlled, improve operating efficiency.
It should be noted that only for representing each part of liquid-adding device 4 and the annexation of cleaning case in Fig. 6, not
The relative position relation of liquid-adding device 4 and cleaning case is limited, liquid-adding device 4 can be arranged at the either side of cleaning case.
For the ease of discharge opeing, as shown in figure 1, the cleaning case includes outer-layer box body 1 and is arranged in the outer-layer box body 1
Interior layer box body 2, the interior layer box body 2 can be with the synchronized oscillation of outer-layer box body 1, and the outer-layer box body 1 connects with the swing pallet
Connect, as shown in Figure 2, Figure 3, Figure 4, the interior layer box body 2 includes bottom plate 23 and the side plate 24 set for 23 1 weeks around the bottom plate,
Bottom plate 23 is provided with dividing plate 25, and the space in the interior layer box body 2 is divided into multiple rinse baths 21, institute by the dividing plate 25
State outer-layer box body 1 and be connected with discharging tube 11, the discharging tube 11 is connected with the bottom space of the outer-layer box body 1, it is each described
Rinse bath 21 is equipped with outage 211, and the outage 211 is connected with the bottom space of the outer-layer box body 1, the outage
211 allow de- cell reagent by and do not allow biomaterial to pass through.Thus, in discharge opeing, discharging tube 11 only need to be opened, is cleaned
De- cell reagent in groove 21 can be discharged by discharging tube 11, simple to operate without dismantling outer-layer box body 1 and interior layer box body 2, raw
Produce efficiency high.
In addition, interior layer box body 2 can not also use dividing plate 25, multiple independent cleanings are directly placed in interior layer box body 2
Groove 21, multiple lateral walls of rinse bath 21 are fitted each other to be closely arranged in interior layer box body 2.Scenario-frame shown in Fig. 3 it is simple and
It is low therefore relatively more economical and practical to making required precision.
Wherein, the arrangement mode of multiple rinse baths 21 can have a variety of, for example can be along same linear array into one
Row, can also be by multiple rinse baths 21 along rectangular array.It is overall by the rinse bath of rectangular array compared to along linear array
More compact structure, is difficult deformation during vibration.For example, as shown in figure 1, the dividing plate 25 divides the space in the interior layer box body 2
16 rinse baths are divided into, 16 rinse bath arrays are that 4 rows 4 are arranged.Equally, dividing plate 25 can also be by the sky in interior layer box body 2
Between be divided into 9 rinse baths, 9 rinse bath arrays are that 3 rows 3 are arranged.Specifically how to split and arrange can be according to actual conditions
Selected, do not limited herein.
Because during progress is cell free, de- cell reagent can produce foam, and foam, which is saved bit by bit, can excessively influence liquid level
The measurement accuracy of meter, can also influence cell free effect, therefore, as shown in Fig. 2 it is preferred that in the opening of the interior layer box body 2
Place sets and foam adsorption apparatus 5 is set on internal layer case lid 26, the internal layer case lid 26, the foam adsorption apparatus 5 respectively with it is multiple described clear
The upper space connection of washing trough 21, the foam that cell reagent is produced is taken off for drawing in the rinse bath 21.Thus, can be by inhaling
Bulb apparatus 5 draws foam, prevents foam from saving bit by bit excessively.
Specifically, foam adsorption apparatus 5 can be the structure shown in Fig. 5, and the foam adsorption apparatus 5 include multiple inhale and steep pipe 51, many
The individual bubble pipe 51 of inhaling is arranged on the internal layer case lid 26, and multiple first ends one-to-one corresponding for inhaling bubble pipe 51 stretch into multiple
In the rinse bath 21, multiple second ends for inhaling bubble pipe 51 are connected with collection vessel 52, and multiple described inhale are divided on bubble pipe 51
Suction pump 53 is not serially connected with.Thus, multiple bubble pipes 51 of inhaling can respectively be controlled by multiple suction pumps 53, control accuracy is high.
In addition, in another embodiment of the invention, foam adsorption apparatus 5 can be the structure shown in Fig. 2, the suction bubble dress
Putting 5 includes multiple suction bubble pipes 51, and multiple bubble pipes 51 of inhaling are arranged on the internal layer case lid 26, and multiple described inhale steep pipe 51
First end is corresponded and stretched into multiple rinse baths 21, and multiple second ends for inhaling bubble pipe 51 pass through multiple way union 54
Connected with collecting pipe 55, the collecting pipe 55 is connected with the collection vessel 52, the collecting pipe 55 is serially connected with suction pump 53.By
This, need to only set a suction pump 53 that can all rinse baths 21 be carried out with suction bubble, and this scheme saves cost and space.
For the ease of the situation in observation rinse bath 21, preferably the internal layer case lid 26 is made of transparent material.By
This, can observe the situation in rinse bath 21, such as how much is foam, height of liquid level, biomaterial state etc. by internal layer case lid 26.If
Abnormal conditions occur can find in time.
Wherein, in order to realize synchronized oscillation of the interior layer box body 2 with outer-layer box body 1, can by the inside dimension of outer-layer box body 1 and
The external dimensions of interior layer box body 2 matches, i.e., when interior layer box body 2 is installed in outer-layer box body 1, the madial wall of outer-layer box body 1
Bonded to each other between the lateral wall of interior layer box body 2, so that outer-layer box body 1 is during vibration, interior layer box body 2 can not
Moved relative to outer-layer box body 1, it is to avoid shock is produced between outer-layer box body 1 and interior layer box body 2.Alternatively, it is also possible in outer layer case
Location structure is set between body 1 and interior layer box body 2, prevents interior layer box body 2 from being moved relative to outer-layer box body 1 by location structure,
For example rubber blanket can be set between outer-layer box body 1 and interior layer box body 2, outer-layer box body 1 is fixed in one end of rubber blanket
On madial wall, the lateral wall of the other end and interior layer box body 2 is against or one end of rubber blanket being fixed on to the outside of interior layer box body 2
On wall, the madial wall of the other end and outer-layer box body 1 against.It can equally realize that interior layer box body 2 shakes with the synchronization of outer-layer box body 1
Swing.
As shown in figure 3, the upper surface both sides of interior layer box body 2 are provided with handle 22, so that conveniently by interior layer box body 2 by outer layer case
Taken out in body 1, be easy to internal layer box body 2 to carry out disinfection and sterilize and cleaning treatment.
Liquid feeding for convenience, as shown in figure 3, it is preferred that open up intercommunicating pore 251 in the lower end of dividing plate 25, it is two neighboring described clear
Washing trough 21 is connected by the intercommunicating pore 251.Thus, when adding de- cell reagent, it is not necessary to add one by one into rinse bath 21
Enter, only need to add de- cell reagent to a rinse bath 21, de- cell reagent is flowed into other rinse baths 21 by intercommunicating pore 251
.And this scheme may be such that de- cell reagent dosage and concentration in each rinse bath 21 are equal, it is ensured that with a collection of clear
The de- cell effect for the biomaterial washed is consistent.
As shown in figure 3, the setting for one week around rinse bath 21 of intercommunicating pore 251, and vertically it is provided with two rows.Connection
Hole 251 should not be opened up excessively, preferably vertically set 1~3 row, if dividing plate 25 can excessively be reduced to liquid by setting
Flop power so that liquid drives the movement velocity reduction of biomaterial, so as to reduce the bat between biomaterial and dividing plate 25
Power is hit, de- cell effect can be influenceed.
Wherein, the set-up mode of outage 211 can have multiple choices, in an embodiment of the present invention, such as Fig. 4 institutes
Show, outage 211 is distributed on the bottom plate 23 of interior layer box body 2, and corresponding with multiple rinse baths 21 respectively, the bottom of interior layer box body 2
It is formed between plate 23 and the bottom plate 12 of the outer-layer box body 1 and connects chamber 28, multiple institutes on the bottom plate 23 of the interior layer box body 2
Outage 211 is stated to connect with the chamber 28 that connects.Thus, in discharge opeing, open after discharging tube 11, the liquid in rinse bath 21
Sequentially pass through connection chamber 28 and discharging tube 11 is discharged, it is easy to operate, it is not necessary to layer box body 2 in dismounting.
In the above-described embodiments, connection chamber 28 can have a variety of implementations, for example, as shown in Fig. 2 can be in internal layer case
Support member 27 is set between the bottom plate 23 of body 2 and the bottom plate 12 of outer-layer box body 1, by support member 27 by the bottom plate of interior layer box body 2
23 separate with the bottom plate 12 of outer-layer box body 1, make to be formed between the bottom plate 23 of interior layer box body 2 and the bottom plate 12 of outer-layer box body 1 and connect
Chamber 28.Equally, hanging structure can also be set between the side wall of interior layer box body 2 and the side wall of outer-layer box body 1, makes interior layer box body
2 are vacantly mounted in outer-layer box body 1, formation between the bottom plate 23 of interior layer box body 2 and the bottom plate 12 of outer-layer box body 1 is connected chamber
28。
In another embodiment of the invention, outage 211 can also be distributed on the side plate 24 of interior layer box body 2, such as
Shown in Fig. 3, Fig. 4, the lower end of the dividing plate 25 offers intercommunicating pore 251, and the two neighboring rinse bath 21 passes through the connection
Hole 251 is connected, and outage 211 is opened in the lower end of the side plate 24.Thus, in discharge opeing, open after discharging tube 11, in being located at
Liquid in the rinse bath 21 in portion first passes through intercommunicating pore 251 and flowed into outermost rinse bath 21, then passes through the lower end of side plate 24
Outage 211 be discharged into outer-layer box body 1, finally discharged by discharging tube 11, this scheme again without dismounting in layer box body 2.
It should be noted that the plan of establishment of above two outage 211 can only with one kind, can also two kinds simultaneously
Using.When only with the first scheme, during the fluid apertures 211 that begun to rehearse on the bottom plate 23 of interior layer box body 2, outage 211 is easily given birth to
Thing material is blocked, and discharge opeing effect is not good, therefore preferably two schemes are used simultaneously.
Wherein, the diameter of outage 211 is preferred to use 3 millimeters, biomaterial is easily caught in if aperture is too big, if aperture
Too small then drain age velocity is too slow.
In order to realize automatic drain pipe 11, tapping valve can be concatenated on discharging tube 11, discharge opeing can be controlled by control unit
Valve is opened and closed, to realize automatic drain.
In order to realize quick fluid-discharge, positive displacement pump preferably is concatenated on the discharging tube 11, thus, positive displacement pump can be extracted out quickly
De- cell reagent, so as to realize quick fluid-discharge, improves operating efficiency.
Because used de- cell reagent usually contains more impurity, in order to prevent impurity from blocking tapping valve and discharge opeing
Pump, as shown in Fig. 2 it is preferred that setting screen pack 14 in the porch of discharging tube 11, screen pack 14 can will be miscellaneous in de- cell reagent
Matter is filtered out, and prevents impurity from blocking tapping valve and positive displacement pump.
Wherein, the horizontal cross-section of rinse bath 21 can be rectangle, square, polygon circle etc., not limit herein.
Because the oscillation amplitude of typically conventional shaking table 3 is 30~60 millimeters, in order to ensure that rinse bath 21 can adapt to the vibration of shaking table 3
The full-size of the rinse bath 21 in the horizontal direction, is preferably set smaller than or equal to 300 millimeters by amplitude.For example, cleaning
When groove 21 is rectangle, the width of rinse bath 21 can be set to 140 millimeters, length is set to 230 millimeters.
Preferably, the height of the rinse bath 21 vertically should be greater than or equal to 100 millimeters, for example, height may be selected
For 250 millimeters, it thus can prevent the liquid in rinse bath 21 from being spilt in vibration.
Discharge, the bottom plate 12 of the outer-layer box body 1 can be obliquely installed for the ease of liquid, and the outer-layer box body 1
The minimum point of bottom plate 12 is set close to the discharging tube 11.Thus, it may be such that de- cell reagent in outer-layer box body 1 along inclined-plane stream
Move to the porch of discharging tube 11, so as to prevent the bottom of outer-layer box body 1 from remaining de- cell reagent.
In the oscillatory process of shaking table 3, biomaterial is easily got rid of to the opening of interior layer box body 2, and is ridden on dividing plate 25
Edge, so that the biomaterial can not complete de- cell, in order to avoid above mentioned problem, preferably sets the internal layer case lid 26
It is set to plate-like structure, in the upper edge of the side plate 24 and the dividing plate 25 is generally aligned in the same plane, the internal layer case lid 26 is covered
On the side plate 24 and the dividing plate 25.Thus, the upper edge of internal layer case lid 26 and side plate 24 and the dividing plate 25 is close
Laminating so that biomaterial can not ride over dividing plate 25 or the upper edge of side plate 24.As shown in fig. 6, can be set on internal layer case lid 26
Case lid handle 261.
In addition, as shown in figure 1, outer layer case lid 13 can also be set at the opening of outer-layer box body 1, so as to prevent extraneous miscellaneous
Matter enters in de- cell reagent, pollutes biomaterial.
Preferably, the outer-layer box body 1 for taking off cell net cage is fixed on swing pallet by interface arrangment, such as Fig. 1 institutes
Show, the bottom surface of shaking table 3 is provided with retractable roller 31, facilitates the carrying of shaking table 3.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of automatic liquid supply formula takes off cell system, it is characterised in that including：
Shaking table, the shaking table includes swinging pallet；
Cleaning case, the cleaning case is arranged on the swing pallet, and the cleaning case includes multiple rinse baths, the rinse bath
For placing biomaterial and de- cell reagent；
Liquid-adding device, the liquid-adding device includes fluid reservoir, liquid-feeding tube and fluid filling pump, described liquid-feeding tube one end and the fluid reservoir
Connection, the other end is stretched into the rinse bath, and the fluid filling pump is concatenated with the liquid-feeding tube；
Liquid level gauge, the liquid level gauge is used to detect the liquid level in the rinse bath；
Control unit, described control unit is connected with the fluid filling pump and the liquid level gauge respectively, is detected when the liquid level gauge
When liquid level is less than preset value, described control unit controls the fluid filling pump to open.
2. automatic liquid supply formula according to claim 1 takes off cell system, it is characterised in that the cleaning case includes outer layer case
Body and the interior layer box body being arranged in the outer-layer box body, the interior layer box body can be with outer-layer box body synchronized oscillation, the outer layer
Casing be connected with the swing pallet, provided with dividing plate in the interior layer box body, and the dividing plate is by the space in the interior layer box body
It is divided into multiple rinse baths, the outer-layer box body is connected with discharging tube, the discharging tube and the bottom of the outer-layer box body
Space is connected, and each rinse bath is equipped with outage, and the outage is connected with the bottom space of the outer-layer box body, institute
State outage allow de- cell reagent by and do not allow biomaterial to pass through.
3. automatic liquid supply formula according to claim 2 takes off cell system, it is characterised in that at the opening of the interior layer box body
Provided with internal layer case lid, the internal layer case lid is provided with foam adsorption apparatus, and the foam adsorption apparatus are upper with multiple rinse baths respectively
Portion space is connected, and the foam that cell reagent is produced is taken off in the rinse bath for drawing.
4. automatic liquid supply formula according to claim 3 takes off cell system, it is characterised in that the foam adsorption apparatus include multiple
Bubble pipe is inhaled, multiple bubble pipes of inhaling are arranged on the internal layer case lid, and multiple first ends for inhaling bubble pipe, which are corresponded, to be stretched into
In multiple rinse baths, multiple second ends for inhaling bubble pipe are connected with collection vessel, and multiple described inhale are gone here and there respectively on bubble pipe
It is connected to suction pump.
5. automatic liquid supply formula according to claim 3 takes off cell system, it is characterised in that the foam adsorption apparatus include multiple
Bubble pipe is inhaled, multiple bubble pipes of inhaling are arranged on the internal layer case lid, and multiple first ends for inhaling bubble pipe, which are corresponded, to be stretched into
In multiple rinse baths, multiple second ends for inhaling bubble pipe are connected by multiple way union with collecting pipe, the collecting pipe
Connected with the collection vessel, the collecting pipe is serially connected with suction pump.
6. automatic liquid supply formula according to claim 3 takes off cell system, it is characterised in that the internal layer case lid is by transparent material
Material is made.
7. cell system is taken off according to automatic liquid supply formula according to any one of claims 1 to 6, it is characterised in that the internal layer
Casing includes bottom plate and the side plate set for one week around bottom plate, and the dividing plate is arranged on the bottom plate, and the outage is many
Individual, at least a portion outage is opened on the bottom plate of the interior layer box body, the bottom plate of the interior layer box body with it is described outer
The multiple outages being formed between the bottom plate of layer box body on connection chamber, the bottom plate of the interior layer box body pass through the connection
Chamber is connected.
8. automatic liquid supply formula according to claim 7 takes off cell system, it is characterised in that the lower end of the dividing plate is offered
Intercommunicating pore, the two neighboring rinse bath is connected by the intercommunicating pore.
9. automatic liquid supply formula according to claim 8 takes off cell system, it is characterised in that at least a portion outage
It is opened in the lower end of the side plate.
10. the automatic liquid supply formula according to any one of claim 2~6 takes off cell system, it is characterised in that the outer layer
The bottom plate of casing is obliquely installed, and the minimum point of the bottom plate of the outer-layer box body is set close to the discharging tube.
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| CN107158464B (en) | 2020-10-30 |
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