CN106963985A - A kind of de- cell net cage and de- cell device - Google Patents
A kind of de- cell net cage and de- cell device Download PDFInfo
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- CN106963985A CN106963985A CN201710402171.0A CN201710402171A CN106963985A CN 106963985 A CN106963985 A CN 106963985A CN 201710402171 A CN201710402171 A CN 201710402171A CN 106963985 A CN106963985 A CN 106963985A
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- 239000012620 biological material Substances 0.000 claims abstract description 37
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 238000007599 discharging Methods 0.000 claims abstract description 21
- 230000010355 oscillation Effects 0.000 claims abstract description 14
- 230000001360 synchronised effect Effects 0.000 claims abstract description 9
- 239000006260 foam Substances 0.000 claims description 15
- 239000011148 porous material Substances 0.000 claims description 9
- 238000001179 sorption measurement Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 abstract description 6
- 238000009434 installation Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 64
- 239000007788 liquid Substances 0.000 description 23
- 238000004140 cleaning Methods 0.000 description 15
- 239000012530 fluid Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003491 array Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003534 oscillatory effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3629—Intestinal tissue, e.g. small intestinal submucosa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3625—Vascular tissue, e.g. heart valves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/005—Ingredients of undetermined constitution or reaction products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Animal Behavior & Ethology (AREA)
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- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Vascular Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Urology & Nephrology (AREA)
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Abstract
The invention discloses a kind of de- cell net cage and de- cell device, it is related to biological material deal device technical field.Taking off cell device to solve prior art can not adapt to produce in enormous quantities and invent the problem of installation and removal process is more complicated.The de- cell net cage of the present invention, including outer-layer box body and interior layer box body, outer-layer box body is used to be connected with shaking table, the interior layer box body can be with outer-layer box body synchronized oscillation, multiple rinse baths for being used to place biomaterial and de- cell reagent are formed with the interior layer box body, the outer-layer box body is connected with discharging tube, the discharging tube is connected with the bottom space of the outer-layer box body, each rinse bath is equipped with outage, the outage is connected with the bottom space of the outer-layer box body, the outage allow de- cell reagent by and do not allow biomaterial to pass through.The de- cell net cage of the present invention can be used for biomaterial to take off cell.
Description
Technical field
The present invention relates to biological material deal device technical field, more particularly to a kind of de- cell net cage and de- cell dress
Put.
Background technology
With the development of economy and society, the application of biomaterial medically also seems more and more prominent, biomaterial
Refer to the process of the material formed by biology, including cell and extracellular matrix.Conventional biomaterial have bovine pericardium and
Chitterlings, when biomaterial is applied into clinical, it is necessary to be cleaned to biomaterial, sterilizing, de- cell etc. are handled
Process.
In the prior art, de- cell is generally carried out using shaking table, specifically, a cleaning is fixed on the pallet of shaking table
Box, is put into biomaterial and de- cell reagent in cleaning box, is then turned on shaking table and is vibrated, in oscillatory process, cleaning
Box inwall is flopped repeatedly to biomaterial, so as to realize the de- cell of biomaterial.
But, because the oscillation amplitude of shaking table is limited, the volume of cleaning box can not be made big, can make if volume is too big
Cleaning box two lateral walls spacing it is very big, thus, biomaterial cleaning box vibrate during also not with cleaning box
Inwall produces slap, and just warp-wise opposite direction has been moved, so that de- cell can not be realized.Therefore, the de- cell cleaning of prior art
Box, in order to adapt to the oscillation amplitude of shaking table, usual volume is all smaller, it is impossible to adapt to produce in enormous quantities.If on the pallet of shaking table
Simultaneously fixed multiple independent cleaning box, then when discharging liquid (taking off cell reagent), it is necessary to which cleaning box is removed one by one after
Liquid in cleaning box is outwelled, installation and removal process is more complicated, so as to cause production efficiency to decline.
The content of the invention
Embodiments of the invention provide a kind of de- cell net cage and de- cell device, can increase the processing number of biomaterial
Amount, and discharge opeing is simple to operate, production efficiency is high.
To reach above-mentioned purpose, The embodiment provides a kind of de- cell net cage, for being carried out to biomaterial
De- cell, including outer-layer box body and the interior layer box body being arranged in the outer-layer box body, the outer-layer box body is used for and shaking table connects
Connect, the interior layer box body can be formed with multiple for placing biomaterial with outer-layer box body synchronized oscillation in the interior layer box body
With the rinse bath of de- cell reagent, the outer-layer box body is connected with discharging tube, the discharging tube and the bottom of the outer-layer box body
Space is connected, and each rinse bath is equipped with outage, and the outage is connected with the bottom space of the outer-layer box body, institute
State outage allow de- cell reagent by and do not allow biomaterial to pass through.
Another aspect of the present invention additionally provides a kind of de- cell device, including shaking table, and the shaking table includes swinging pallet, institute
State and swing pallet provided with the de- cell net cage described in any of the above-described technical scheme.
The de- cell net cage of the present invention and de- cell device, because outer-layer box body is used to be connected with shaking table, the interior layer box body
Can be with outer-layer box body synchronized oscillation, therefore when outer-layer box body vibrates, interior layer box body can be with outer-layer box body synchronized oscillation, so that real
The now de- cell processing of the biomaterial in internal layer box body, and multiple for placing biology due to being formed with interior layer box body
The rinse bath of material and de- cell reagent, therefore, the volume of each rinse bath can be made as adapting to shaking table oscillation amplitude most
Large volume, compared with a cleaning box of prior art, increases the processing quantity of biomaterial.And because outer-layer box body connects
Discharging tube is connected to, multiple rinse baths are equipped with outage, therefore in discharge opeing, need to only open de- thin in discharging tube, rinse bath
Born of the same parents' reagent can be discharged by discharging tube, simple to operate without dismantling outer-layer box body and interior layer box body, and production efficiency is high.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the overall structure diagram that the embodiment of the present invention takes off cell net cage；
Fig. 2 is the internal structure schematic diagram that the embodiment of the present invention takes off cell net cage；
Fig. 3 is the structural representation for the outer-layer box body that the embodiment of the present invention takes off cell net cage；
Fig. 4 is the structural representation for the interior layer box body that the embodiment of the present invention takes off cell net cage；
Fig. 5 is the polycrystalline substance schematic diagram for the interior layer box body that the embodiment of the present invention takes off cell net cage；
Fig. 6 is the structural representation that the de- cell net cage of the embodiment of the present invention has liquid-adding device and foam adsorption apparatus；
Fig. 7 is the structural representation of another embodiment for the foam adsorption apparatus that the embodiment of the present invention takes off cell net cage；
Fig. 8 is the structural representation for the internal layer case lid that the embodiment of the present invention takes off cell net cage.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
In the description of the invention, it is to be understood that term " " center ", " on ", " under ", "front", "rear", " left side ",
The orientation or position relationship of the instruction such as " right side ", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are based on shown in the drawings
Orientation or position relationship, are for only for ease of the description present invention and simplify description, rather than indicate or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In description of the invention, unless otherwise indicated, " multiple " are meant that two or more.
Reference picture 1, Fig. 2, Fig. 1 and Fig. 2 are the specific embodiment that the embodiment of the present invention takes off cell net cage, the present embodiment
De- cell net cage, for carrying out de- cell to biomaterial, including outer-layer box body 1 and be arranged in the outer-layer box body 1
Interior layer box body 2, the outer-layer box body 1 is used to be connected with shaking table 3, and the interior layer box body 2 can be with the synchronized oscillation of outer-layer box body 1, institute
State and multiple rinse baths 21 for being used to place biomaterial and de- cell reagent are formed with interior layer box body 2, the outer-layer box body 1 connects
Discharging tube 11 is connected to, the discharging tube 11 is connected with the bottom space of the outer-layer box body 1, each rinse bath 21 is equipped with
Outage 211, the outage 211 is connected with the bottom space of the outer-layer box body 1, and the outage 211 allows de- cell
Reagent by and do not allow biomaterial to pass through.
De- cell net cage provided in an embodiment of the present invention, because outer-layer box body 1 is used to be connected with shaking table, the interior layer box body
2 can with the synchronized oscillation of outer-layer box body 1, therefore when outer-layer box body 1 vibrates, interior layer box body 2 can with the synchronized oscillation of outer-layer box body 1,
So as to realize the de- cell processing of the biomaterial in internal layer box body 2, and due to being formed with multiple be used in interior layer box body 2
The rinse bath 21 of biomaterial and de- cell reagent is placed, therefore, the volume of each rinse bath 21 can be made as adapting to shaking table
The maximum volume of oscillation amplitude, compared with a cleaning box of prior art, increases the processing quantity of biomaterial.And by
Discharging tube 11 is connected with outer-layer box body 1, multiple rinse baths 21 are equipped with outage 211, therefore in discharge opeing, only need to the row of opening
De- cell reagent in liquid pipe 11, rinse bath 21 can be discharged by discharging tube 11, without dismantling outer-layer box body 1 and interior layer box body 2,
Simple to operate, production efficiency is high.
Wherein, in order to realize synchronized oscillation of the interior layer box body 2 with outer-layer box body 1, can by the inside dimension of outer-layer box body 1 and
The external dimensions of interior layer box body 2 matches, i.e., when interior layer box body 2 is installed in outer-layer box body 1, the madial wall of outer-layer box body 1
Bonded to each other between the lateral wall of interior layer box body 2, so that outer-layer box body 1 is during vibration, interior layer box body 2 can not
Moved relative to outer-layer box body 1, it is to avoid shock is produced between outer-layer box body 1 and interior layer box body 2.Alternatively, it is also possible in outer layer case
Location structure is set between body 1 and interior layer box body 2, prevents interior layer box body 2 from being moved relative to outer-layer box body 1 by location structure,
For example rubber blanket can be set between outer-layer box body 1 and interior layer box body 2, outer-layer box body 1 is fixed in one end of rubber blanket
On madial wall, the lateral wall of the other end and interior layer box body 2 is against or one end of rubber blanket being fixed on to the outside of interior layer box body 2
On wall, the madial wall of the other end and outer-layer box body 1 against.It can equally realize that interior layer box body 2 shakes with the synchronization of outer-layer box body 1
Swing.
As shown in figure 4, the upper surface both sides of interior layer box body 2 are provided with handle 22, so that conveniently by interior layer box body 2 by outer layer case
Taken out in body 1, be easy to internal layer box body 2 to carry out disinfection and sterilize and cleaning treatment.
Specifically, interior layer box body 2 can be using structure as shown in Figure 4, Figure 5, i.e., including bottom plate 23 and around the bottom
Dividing plate 25 is provided with the side plate 24 that plate is set for 23 1 weeks, the interior layer box body 2, the dividing plate 25 is by the interior layer box body 2
Space is divided into multiple rinse baths 21.Alternatively, it is also possible to not use dividing plate 25, directly place multiple in interior layer box body 2
Independent rinse bath 21, multiple lateral walls of rinse bath 21 are fitted each other to be closely arranged in interior layer box body 2.Scheme shown in Fig. 4
It is simple in construction and low therefore relatively more economical and practical to making required precision.
Wherein, the arrangement mode of multiple rinse baths 21 can have a variety of, for example can be along same linear array into one
Row, can also be by multiple rinse baths 21 along rectangular array.It is overall by the rinse bath of rectangular array compared to along linear array
More compact structure, is difficult deformation during vibration.For example, as shown in figure 1, the dividing plate 25 divides the space in the interior layer box body 2
16 rinse baths are divided into, 16 rinse bath arrays are that 4 rows 4 are arranged.Equally, dividing plate 25 can also be by the sky in interior layer box body 2
Between be divided into 9 rinse baths, 9 rinse bath arrays are that 3 rows 3 are arranged.Specifically how to split and arrange can be according to actual conditions
Selected, do not limited herein.
Liquid feeding for convenience, as shown in figure 4, it is preferred that open up intercommunicating pore 251 in the lower end of dividing plate 25, it is two neighboring described clear
Washing trough 21 is connected by the intercommunicating pore 251.Thus, when adding de- cell reagent, it is not necessary to add one by one into rinse bath 21
Enter, only need to add de- cell reagent to a rinse bath 21, de- cell reagent is flowed into other rinse baths 21 by intercommunicating pore 251
.And this scheme may be such that de- cell reagent dosage and concentration in each rinse bath 21 are equal, it is ensured that with a collection of clear
The de- cell effect for the biomaterial washed is consistent.
As shown in figure 4, the setting for one week around rinse bath 21 of intercommunicating pore 251, and vertically it is provided with two rows.Connection
Hole 251 should not be opened up excessively, preferably vertically set 1~3 row, if dividing plate 25 can excessively be reduced to liquid by setting
Flop power so that liquid drives the movement velocity reduction of biomaterial, so as to reduce the bat between biomaterial and dividing plate 25
Power is hit, de- cell effect can be influenceed.
Wherein, the set-up mode of outage 211 can have multiple choices, in an embodiment of the present invention, such as Fig. 5 institutes
Show, outage 211 is distributed on the bottom plate 23 of interior layer box body 2, and corresponding with multiple rinse baths 21 respectively, the bottom of interior layer box body 2
It is formed between plate 23 and the bottom plate 12 of the outer-layer box body 1 and connects chamber 28, multiple institutes on the bottom plate 23 of the interior layer box body 2
Outage 211 is stated to connect with the chamber 28 that connects.Thus, in discharge opeing, open after discharging tube 11, the liquid in rinse bath 21
Sequentially pass through connection chamber 28 and discharging tube 11 is discharged, it is easy to operate, it is not necessary to layer box body 2 in dismounting.
In the above-described embodiments, connection chamber 28 can have a variety of implementations, for example, as shown in Fig. 2 can be in internal layer case
Support member 27 is set between the bottom plate 23 of body 2 and the bottom plate 12 of outer-layer box body 1, by support member 27 by the bottom plate of interior layer box body 2
23 separate with the bottom plate 12 of outer-layer box body 1, make to be formed between the bottom plate 23 of interior layer box body 2 and the bottom plate 12 of outer-layer box body 1 and connect
Chamber 28.Equally, hanging structure can also be set between the side wall of interior layer box body 2 and the side wall of outer-layer box body 1, makes interior layer box body
2 are vacantly mounted in outer-layer box body 1, formation between the bottom plate 23 of interior layer box body 2 and the bottom plate 12 of outer-layer box body 1 is connected chamber
28。
In another embodiment of the invention, outage 211 can also be distributed on the side plate 24 of interior layer box body 2, such as
Shown in Fig. 4, the lower end of the dividing plate 25 offers intercommunicating pore 251, and the two neighboring rinse bath 21 passes through the intercommunicating pore 251
Connection, outage 211 is opened in the lower end of the side plate 24.Thus, in discharge opeing, open after discharging tube 11, positioned at middle part
Liquid in rinse bath 21 first passes through intercommunicating pore 251 and flowed into outermost rinse bath 21, then passes through the row of the lower end of side plate 24
Fluid apertures 211 is discharged into outer-layer box body 1, is finally discharged by discharging tube 11, and this scheme is again without layer box body 2 in dismounting.
It should be noted that the plan of establishment of above two outage 211 can only with one kind, can also two kinds simultaneously
Using.When only with the first scheme, during the fluid apertures 211 that begun to rehearse on the bottom plate 23 of interior layer box body 2, outage 211 is easily given birth to
Thing material is blocked, and discharge opeing effect is not good, therefore preferably two schemes are used simultaneously.
Wherein, the diameter of outage 211 is preferred to use 3 millimeters, biomaterial is easily caught in if aperture is too big, if aperture
Too small then drain age velocity is too slow.
Wherein, the horizontal cross-section of rinse bath 21 can be rectangle, square, polygon circle etc., not limit herein.
Because the oscillation amplitude of typically conventional shaking table 3 is 30~60 millimeters, in order to ensure that rinse bath 21 can adapt to the vibration of shaking table 3
The full-size of the rinse bath 21 in the horizontal direction, is preferably set smaller than or equal to 300 millimeters by amplitude.For example, cleaning
When groove 21 is rectangle, the width of rinse bath 21 can be set to 140 millimeters, length is set to 230 millimeters.
Preferably, the height of the rinse bath 21 vertically should be greater than or equal to 100 millimeters, for example, height may be selected
For 250 millimeters, it thus can prevent the liquid in rinse bath 21 from being spilt in vibration.
De- cell reagent is added into rinse bath 21 for convenience, as shown in fig. 6, liquid-adding device 4 can also be set, it is described
Liquid-adding device 4 includes fluid reservoir 41, liquid-feeding tube 42 and fluid filling pump 43, and described one end of liquid-feeding tube 42 is connected with the fluid reservoir 41,
The other end is stretched into the rinse bath 21, and the fluid filling pump 43 is concatenated with the liquid-feeding tube 42；Liquid level gauge is additionally provided with (in figure not show
Go out), the liquid level gauge is used to detect the liquid level in the rinse bath 21；Liquid-adding device controls liquid feeding by control unit, described
Control unit is connected with the fluid filling pump 43 and the liquid level gauge respectively, when the liquid level that the liquid level gauge is detected is less than preset value
When, described control unit controls the fluid filling pump 43 to open.Therefore, when the liquid level that the liquid level gauge is detected is less than preset value,
Control unit can control the fluid filling pump 43 and open, and the de- cell reagent in fluid reservoir 41 is pumped into rinse bath 21, when reaching
Fluid filling pump 43 is closed when specifying liquid level.Hereby it is achieved that automatic liquid supply, and the amount of reagent of addition can be accurately controlled, carry
High operating efficiency.
It should be noted that only for representing each part of liquid-adding device 4 and the annexation of outer-layer box body in Fig. 6, and
The relative position relation of liquid-adding device 4 and outer-layer box body is not limited, and liquid-adding device 4 can be arranged at any the one of outer-layer box body
Side.
Because during progress is cell free, de- cell reagent can produce foam, and foam, which is saved bit by bit, can excessively influence liquid level
The measurement accuracy of meter, can also influence cell free effect, therefore, as shown in fig. 6, it is preferred that in the opening of the interior layer box body 2
Place sets and foam adsorption apparatus 5 is set on internal layer case lid 26, the internal layer case lid 26, the foam adsorption apparatus 5 respectively with it is multiple described clear
The upper space connection of washing trough 21, the foam that cell reagent is produced is taken off for drawing in the rinse bath 21.Thus, can be by inhaling
Bulb apparatus 5 draws foam, prevents foam from saving bit by bit excessively.
Specifically, foam adsorption apparatus 5 can be the structure shown in Fig. 7, and the foam adsorption apparatus 5 include multiple inhale and steep pipe 51, many
The individual bubble pipe 51 of inhaling is arranged on the internal layer case lid 26, and multiple first ends one-to-one corresponding for inhaling bubble pipe 51 stretch into multiple
In the rinse bath 21, multiple second ends for inhaling bubble pipe 51 are connected with collection vessel 52, and multiple described inhale are divided on bubble pipe 51
Suction pump 53 is not serially connected with.Thus, multiple bubble pipes 51 of inhaling can respectively be controlled by multiple suction pumps 53, control accuracy is high.
In addition, in another embodiment of the invention, being managed as shown in fig. 6, the foam adsorption apparatus 5 include multiple bubbles of inhaling
51, multiple bubble pipes 51 of inhaling are arranged on the internal layer case lid 26, and multiple first ends for inhaling bubble pipe 51, which are corresponded, stretches
Enter in multiple rinse baths 21, multiple second ends for inhaling bubble pipe 51 are connected by multiple way union 54 with collecting pipe 55,
The collecting pipe 55 is connected with the collection vessel 52, and the collecting pipe 55 is serially connected with suction pump 53.Thus, one need to only be set
Suction pump 53 can carry out suction bubble to all rinse baths 21, and this scheme saves cost and space.
Discharge, the bottom plate 12 of the outer-layer box body 1 can be obliquely installed, and make the outer-layer box body 1 for the ease of liquid
The minimum point of bottom plate 12 set close to the discharging tube 11.Thus, it may be such that de- cell reagent in outer-layer box body 1 along inclined-plane
The porch of discharging tube 11 is flowed to, so as to prevent the bottom of outer-layer box body 1 from remaining de- cell reagent.
In shaking table oscillatory process, biomaterial is easily got rid of to the opening of interior layer box body 2, and rides over the top of dividing plate 25
Edge, so that the biomaterial can not complete de- cell, in order to avoid above mentioned problem, is preferably set the internal layer case lid 26
For plate-like structure, in the upper edge of the side plate 24 and the dividing plate 25 is generally aligned in the same plane, the lid of internal layer case lid 26 is set
In on the side plate 24 and the dividing plate 25.Internal layer case lid 26 is picked and placeed for convenience, as shown in figure 8, can be on internal layer case lid 26
Case lid handle 261 is set.
For the ease of the situation in observation rinse bath 21, preferably the internal layer case lid 26 is made of transparent material.By
This, can observe the situation in rinse bath 21, such as how much is foam, height of liquid level, biomaterial state etc. by internal layer case lid 26.If
Abnormal conditions occur can find in time.
In addition, as shown in figure 3, outer layer case lid 13 can also be set at the opening of outer-layer box body 1, so as to prevent extraneous miscellaneous
Matter enters in de- cell reagent, pollutes biomaterial.
Reference picture 1, embodiments of the invention additionally provide a kind of de- cell device, including shaking table, and the shaking table includes pendulum
Dynamic pallet (not shown), the swing pallet is provided with the de- cell net cage described in any of the above-described technical scheme.
Due to each implementation of the de- cell net cage that is used in the de- cell device of the present embodiment and above-mentioned de- cell net cage
The de- cell net cage provided in example is identical, therefore the two can solve the problem that identical technical problem, and reaches identical Expected Results.
Specifically, the outer-layer box body 1 for taking off cell net cage is fixed on swing pallet by interface arrangment, such as Fig. 1 institutes
Show, the bottom surface of shaking table is provided with retractable roller 31, facilitates the carrying of shaking table.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of de- cell net cage, for carrying out de- cell to biomaterial, it is characterised in that including outer-layer box body and be arranged at
Interior layer box body in the outer-layer box body, the outer-layer box body is used to be connected with shaking table, and the interior layer box body can be with outer-layer box body
Multiple rinse baths for being used to place biomaterial and de- cell reagent are formed with synchronized oscillation, the interior layer box body, it is described outer
Layer box body is connected with discharging tube, and the discharging tube is connected with the bottom space of the outer-layer box body, and each rinse bath is all provided with
There is outage, the outage is connected with the bottom space of the outer-layer box body, the outage allows de- cell reagent to pass through
And do not allow biomaterial to pass through.
2. de- cell net cage according to claim 1, it is characterised in that the interior layer box body includes bottom plate and around described
Dividing plate is provided with the side plate that bottom plate is set for one week, the interior layer box body, the dividing plate separates the space in the interior layer box body
For multiple rinse baths.
3. de- cell net cage according to claim 2, it is characterised in that the outage is multiple, at least a portion institute
State outage to be opened on the bottom plate of the interior layer box body, between the bottom plate of the interior layer box body and the bottom plate of the outer-layer box body
The multiple outages being formed with connection chamber, the bottom plate of the interior layer box body connect chamber connection with described.
4. the de- cell net cage according to Claims 2 or 3, it is characterised in that the lower end of the dividing plate offers intercommunicating pore,
The two neighboring rinse bath is connected by the intercommunicating pore.
5. de- cell net cage according to claim 4, it is characterised in that at least a portion outage is opened in described
The lower end of side plate.
6. de- cell net cage according to claim 1, it is characterised in that the full-size of the rinse bath in the horizontal direction
Less than or equal to 300 millimeters, the height of the rinse bath vertically is more than or equal to 100 millimeters.
7. according to de- cell net cage according to any one of claims 1 to 3, it is characterised in that the bottom plate of the outer-layer box body
It is obliquely installed, and the minimum point of the bottom plate of the outer-layer box body is set close to the discharging tube.
8. the de- cell net cage according to Claims 2 or 3, it is characterised in that provided with interior at the opening of the interior layer box body
Layer case lid, the internal layer case lid is plate-like structure, in the upper edge of the side plate and the dividing plate is generally aligned in the same plane, described
Internal layer case lid is covered on the side plate and the dividing plate.
9. de- cell net cage according to claim 8, it is characterised in that be provided with foam adsorption apparatus on the internal layer case lid,
The foam adsorption apparatus include multiple bubbles of inhaling and managed, and multiple bubble pipes of inhaling are arranged on the internal layer case lid, and multiple bubbles of inhaling are managed
First end correspond stretch into multiple rinse baths, it is multiple it is described inhale bubble pipes the second ends pass through multiple way union with receive
Header in communication, the collecting pipe is serially connected with suction pump.
10. a kind of de- cell device, it is characterised in that including shaking table, the shaking table includes swinging on pallet, the swing pallet
Provided with de- cell net cage according to any one of claims 1 to 9.
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| CN201710402171.0A CN106963985B (en) | 2017-05-31 | 2017-05-31 | Decellularization net cage and decellularization device |
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| CN201710402171.0A CN106963985B (en) | 2017-05-31 | 2017-05-31 | Decellularization net cage and decellularization device |
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| CN106963985B CN106963985B (en) | 2020-09-18 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205019474U (en) * | 2015-09-29 | 2016-02-10 | 陕西瑞盛生物科技有限公司 | Anal fistula repair materials's belt cleaning device |
| CN106267343A (en) * | 2015-05-29 | 2017-01-04 | 深圳艾尼尔角膜工程有限公司 | A kind of cornea takes off cell system and method for removing cells |
| WO2017017474A1 (en) * | 2015-07-30 | 2017-02-02 | Ucl Business Plc | Methods and devices for the production of decellularised tissue scaffolds |
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2017
- 2017-05-31 CN CN201710402171.0A patent/CN106963985B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267343A (en) * | 2015-05-29 | 2017-01-04 | 深圳艾尼尔角膜工程有限公司 | A kind of cornea takes off cell system and method for removing cells |
| WO2017017474A1 (en) * | 2015-07-30 | 2017-02-02 | Ucl Business Plc | Methods and devices for the production of decellularised tissue scaffolds |
| CN205019474U (en) * | 2015-09-29 | 2016-02-10 | 陕西瑞盛生物科技有限公司 | Anal fistula repair materials's belt cleaning device |
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Denomination of invention: A decellularized cage and decellularization device Effective date of registration: 20230711 Granted publication date: 20200918 Pledgee: Xi'an innovation financing Company limited by guarantee Pledgor: SHAANXI RUISHENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023610000548 |
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Granted publication date: 20200918 Pledgee: Xi'an innovation financing Company limited by guarantee Pledgor: SHAANXI RUISHENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023610000548 |