CN215713048U

CN215713048U - Biological cell culture device

Info

Publication number: CN215713048U
Application number: CN202122013156.1U
Authority: CN
Inventors: 戴世诚
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-25
Filing date: 2021-08-25
Publication date: 2022-02-01
Anticipated expiration: 2031-08-25

Abstract

The invention discloses a biological cell culture device which comprises an incubator and a water circulation box, wherein a sealing device is arranged in the incubator and comprises a push-pull cylinder, a fixing plate, a fixing rod and a sealing plate. The push-pull air cylinder is arranged at the bottom of the incubator, and a piston rod of the push-pull air cylinder is vertically arranged upwards. The fixed plate is arranged on a piston rod of the push-pull cylinder. One end of the fixed rod is connected to the fixed plate, and the other end of the fixed rod is connected to the sealing plate. The closing plate hugs closely the incubator lateral wall setting, and the liquid outlet has been seted up to incubator lateral wall bottom, and the closing plate setting is in the liquid outlet top. The invention has the technical effect of protecting the experimental environment in the culture dish when the culture solution is added.

Description

Biological cell culture device

Technical Field

The invention relates to the technical field of cell culture, in particular to a biological cell culture device.

Background

Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched. The environments and conditions required for cell culture include: a sterile environment, a proper temperature, a proper osmotic pressure, a proper gas environment, a proper pH value and the like, and a cell culture device is necessarily required.

However, in the process of implementing the technical solution of the invention in the embodiments of the present application, the inventors of the present application find that at least the following technical problems exist: carry out cell culture in the incubator, when cultivateing, often need add the culture solution to culture dish or culture dish, and the incubator is because generally airtight, and experimental environment is higher to contents requirements such as humiture, oxygen, carbon dioxide in the incubator moreover, but when adding the nutrient solution, need open the culture dish, then can produce adverse effect to the environment in the culture dish, influence culture capacity and culture effect. Accordingly, a biological cell culture apparatus has been proposed to address the above problems.

Disclosure of Invention

The embodiment of this application is through providing a biological cell culture device, when having solved among the prior art toward adding the culture solution in the incubator, because the incubator is airtight generally, when cultivateing biological cell moreover to the humiture in the culture dish, oxygen, content requirements such as carbon dioxide are higher, but when adding the nutrient solution, need open the culture dish, then can produce adverse effect to the environment in the culture dish, influence culture capacity and culture effect, realized when adding the culture solution, the technical effect who tests the environment in the protection culture dish.

The embodiment of the application provides a biological cell culture device, including incubator, hydrologic cycle case, be provided with sealing device in the incubator, sealing device includes push-and-pull cylinder, fixed plate, dead lever and closing plate. The push-pull air cylinder is arranged at the bottom of the incubator, and a piston rod of the push-pull air cylinder is arranged vertically upwards. The fixed plate is arranged on a piston rod of the push-pull air cylinder. One end of the fixed rod is connected to the fixed plate, and the other end of the fixed rod is connected to the sealing plate. The sealing plate is arranged on the side wall of the incubator in a sliding mode, a liquid outlet is formed in the bottom of the side wall of the incubator, and the sealing plate is arranged above the liquid outlet.

Furthermore, a sealing shell is arranged at the bottom of the incubator, the push-pull air cylinder is arranged in the sealing shell, an inlet and outlet hole is formed in the upper portion of the sealing shell, a piston rod of the push-pull air cylinder penetrates through the inlet and outlet hole, and the fixing plate is arranged above the sealing shell.

Furthermore, the fixing plate is composed of four fixing blocks which are arranged oppositely, and the fixing blocks are mutually independent.

Further, set up the culture dish fixing base in the hydrologic cycle case, install the culture dish on the culture dish fixing base, be connected with the drainage tube on the culture dish, the drainage tube with the liquid outlet is connected.

Furthermore, a constant temperature device is arranged outside the water circulation box, and the constant temperature device comprises a water tank, an operation panel, a heating pipe, a water pump, a water delivery pipe and a second water inlet pipe. The water tank is arranged on one side of the water circulation tank, and the water tank is communicated with the water circulation tank through the water delivery pipe and the second water inlet pipe. The operation panel is installed on a side wall of the water tank. The heating pipe is arranged at the bottom of the water tank and electrically connected with the operation panel. The water pump is arranged at the bottom of the water tank and is connected with the water pipe.

The technical scheme provided in the embodiment of the application at least has the following technical effects or advantages:

1. owing to set up sealing device in the incubator, so carry the culture solution to the incubator in advance earlier, sealing device seals the culture dish this moment, stays a period when the culture solution in the incubator, opens sealing device again when incubator adjustment incasement humiture, oxygen, carbon dioxide etc. carry the culture solution to the culture dish in, has effectively avoided directly to add the problem appearance of the environment in the culture solution influence culture dish toward the culture dish in.

2. Because the sealing shell is arranged outside the push-pull air cylinder, the problem that the push-pull air cylinder is rusted due to the fact that the air cylinder is soaked in liquid in the incubator is effectively solved, and the service time of the push-pull air cylinder is greatly prolonged.

3. Because the thermostatic device is arranged beside the culture box, the temperature of the culture dish is ensured, the thermostatic device of the water circulation can repeatedly utilize water in the water tank, the resource waste is avoided, and the effect of environmental protection is achieved.

Drawings

FIG. 1 is a schematic view of the entirety of an embodiment of the present application;

FIG. 2 is a schematic view showing the installation of an apparatus in a water circulation tank according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of the entirety of an embodiment of the present application;

FIG. 4 is a schematic structural view of the inside of a thermostat device according to an embodiment of the present application;

in the figure: 1. an incubator; 11. a liquid outlet; 12. sealing the shell; 121. an inlet and outlet hole; 13. a liquid inlet pipe; 14. a tube cover; 15. a switch; 16. a supporting seat; 2. a sealing device; 21. a push-pull cylinder; 22. a fixing plate; 221. a fixed block; 23. fixing the rod; 24. a sealing plate; 3. a water circulation tank; 31. a culture dish fixing seat; 4. a culture dish; 41. a drainage tube; 5. a thermostatic device; 51. a water tank; 511. a first water inlet pipe; 512. sealing the cover; 52. an operation panel; 53. heating a tube; 54. a water pump; 55. a water delivery pipe; 56. a second water inlet pipe.

Detailed Description

The embodiment of the application discloses biological cell culture device, through set up sealing device 2 in incubator 1, sealing device 2 drives fixed plate 22 through push-and-pull cylinder 21 and reciprocates, and fixed rod 23 connects fixed plate 22 and closing plate 24, so closing plate 24 can move along with the removal of fixed plate 22, and closing plate 24 sets up the top at liquid outlet 11. When culture solution needs to be poured into the incubator 1, the sealing device 2 drives the sealing plate 24 to seal the liquid outlet 11 through the push-pull air cylinder 21, so that the culture dish 4 is isolated from the incubator 1, and the purpose of protecting the environment inside the culture dish 4 is achieved.

In order to better understand the technical solution, the technical solution will be described in detail with reference to the drawings and the specific embodiments.

Referring to fig. 1 and 2, a biological cell culture apparatus includes an incubator 1, a water circulation tank 3, a sealing device 2, a culture dish 4, and a thermostat device 5. Wherein a sealing device 2 is arranged in the incubator 1, which sealing device 2 in certain cases isolates the incubator 1 from the water circulation tank 3. The culture dish 4 is fixedly arranged in the water circulation box 3, the culture dish 4 is communicated with the culture box 1, and the biological cells are cultured in the culture dish 4. The constant temperature device 5 is arranged at one side of the water circulation box 3, the constant temperature device 5 is communicated with the water circulation box 3, and the constant temperature device 5 is responsible for maintaining the whole cell culture device at a constant temperature suitable for culturing cells.

Referring to fig. 1, incubator 1 is the rectangle, and incubator 1 top fixed mounting has feed liquor pipe 13, and feed liquor pipe 13 runs through incubator 1 top, and feed liquor pipe 13 top is installed with feed liquor pipe 13 threaded connection's tube cap 14. When the culture solution is insufficient in the incubator 1, the operator injects the culture solution into the incubator 1 through the liquid inlet pipe 13.

Referring to fig. 1 and 2, the sealing device 2 includes a push-pull cylinder 21, a fixing plate 22, a fixing rod 23, and a sealing plate 24. A sealing shell 12 is fixedly arranged in the middle of the incubator 1, a supporting seat 16 is welded in the middle of the bottom of the sealing shell 12, and a push-pull air cylinder 21 is fixedly installed on the supporting seat 16. The sealed shell 12 prevents the culture solution in the incubator 1 from entering the push-pull air cylinder 21, so that the situation of corrosion of the push-pull air cylinder 21 occurs, the support base 16 enables the push-pull air cylinder 21 to be separated from the bottom of the incubator 1, and even if the culture solution enters the sealed shell 12 carelessly, the culture solution can be collected at the bottom of the sealed shell 12 and cannot be corroded to the push-pull air cylinder 21. The push-pull cylinder 21 is vertically arranged, an access hole 121 is formed above the sealing shell 12, and a piston rod of the push-pull cylinder 21 penetrates through the access hole 121. The switch 15 is fixedly arranged on the top of the incubator 1, and the switch 15 is electrically connected with the push-pull air cylinder 21.

Referring to fig. 2 and 3, the fixing plate 22 is fixedly mounted on the piston rod of the push-pull cylinder 21, and the fixing plate 22 is disposed above the hermetic shell 12. The fixing plate 22 is rectangular, the fixing plate 22 is composed of four fixing blocks 221 which are oppositely arranged, the fixing blocks 221 are triangular, and the fixing blocks 221 are independent. Therefore, if one fixed block 221 fails, the work of other fixed blocks 221 is not affected, and the fault tolerance rate and the work efficiency of the device are improved. The fixing rod 23 is shaped like an irregular Chinese character 'ji', one end of the fixing rod 23 is connected to the fixing plate 22, and the other end of the fixing rod 23 is connected to the sealing plate 24. Every lateral wall bottom of incubator 1 has all been seted up 4 liquid outlets 11, and liquid outlet 11 is tangent with incubator 1 bottom, and closing plate 24 sets up in 11 tops of liquid outlet, and every 11 tops of liquid outlet all have a closing plate 24, and closing plate 24's area is greater than the area of liquid outlet 11, therefore closing plate 24 can easily seal up liquid outlet 11, and closing plate 24 relies on the holding power of dead lever 23 to slide on incubator 1 lateral wall. When the incubator 1 needs to be sealed, the push-pull air cylinder 21 drives the sealing plate 24 through the fixing rod 23 to enable the sealing plate 24 to seal the liquid outlet 11.

Referring to FIG. 3, the water circulation tank 3 has a hollow cylindrical shape, and the water circulation tank 3 surrounds the cultivation box 1. Set up culture dish fixing base 31 in the hydrologic cycle case 3, the joint has culture dish 4 on culture dish fixing base 31, and culture dish 4 and 11 intervals settings of liquid outlet, 11 departments of every liquid outlet all correspond culture dish 4, are connected with drainage tube 41 on the culture dish 4, and culture dish 4 and liquid outlet 11 pass through drainage tube 41 intercommunication. The culture liquid enters the culture dish 4 through the drainage tube 41.

Referring to fig. 3 and 4, the thermostat device 5 includes a water tank 51, an operation panel 52, a heating pipe 53, a water pump 54, a water pipe 55, and a second water inlet pipe 56. The water tank 51 is fixedly installed at one side of the water circulation tank 3, and the water tank 51 is communicated with the water circulation tank 3 through a water delivery pipe 55 and a second water inlet pipe 56. A first water inlet pipe 511 is fixedly arranged at the top of the water tank 51, the first water inlet pipe 511 penetrates through the top of the water tank 51, and a sealing cover 512 in threaded connection with the water inlet pipe is arranged above the water inlet pipe. When the water in the water tank 51 is insufficient, the worker can open the cover 512 to add water into the water tank 51. The operation panel 52 is fixedly installed on a side wall of the water tank 51. The number of the heating pipes 53 is 4, 4 heating pipes 53 are respectively and fixedly arranged at 4 corners of the water tank 51, and the heating pipes 53 are electrically connected with the operation panel 52. The water pump 54 is fixedly arranged at the bottom of the water tank 51, and the water pump 54 is fixedly connected with the water pipe 55. The thermostat device 5 sets a set value to be heated by the heating pipe 53 on the operation panel 52, then the heating pipe 53 starts to work, when the heating pipe 53 is heated to the set value, the water pump 54 starts to convey water in the water tank 51 into the water circulation box 3, so that the temperature in the water circulation box 3 reaches the temperature suitable for the culture dish 4 to culture biological cells, and the water in the water circulation box 3 flows back into the water tank 51 through the second water inlet pipe 56, thereby achieving the effect of recycling the water in the water tank 51, protecting the environment of the device, not wasting water resources, and achieving the recycling of resources.

The functional principle of the present application can be illustrated by the following operation modes:

when the culture solution is poured into the incubator 1, the switch 15 is activated to start the sealing device 2, and the sealing device 2 drives the sealing plate 24 to block the liquid outlet 11 through the push-pull cylinder 21, so that the incubator 1 is in a sealed state. Then the staff opens tube closure 14, puts in culture solution to incubator 1 from feed liquor pipe 13 department, then adjusts the experimental environment in the cultivation, makes it reach and the same as the experimental environment in culture dish 4, starting switch 15, push-and-pull air cylinder 21 drives closing plate 24 rebound to spill liquid outlet 11, the culture solution passes through liquid outlet 11 and gets into in the culture dish 4. Thus, the situation that the culture dish 4 is directly opened, and the experimental environment in the culture dish 4 is influenced by the culture solution in the culture dish 4 is avoided, so that the experimental result is inaccurate.

The above description is only for the preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can substitute or change the technical solution and its concept of the present application within the technical scope disclosed in the present application, and shall be covered by the scope of the present application.

Claims

1. A biological cell culture device comprises an incubator (1) and a water circulation box (3), and is characterized in that a sealing device (2) is arranged in the incubator (1), and the sealing device (2) comprises a push-pull cylinder (21), a fixing plate (22), a fixing rod (23) and a sealing plate (24);

the push-pull air cylinder (21) is arranged at the bottom of the incubator (1), and a piston rod of the push-pull air cylinder (21) is arranged vertically upwards;

the fixed plate (22) is arranged on a piston rod of the push-pull cylinder (21);

one end of the fixing rod (23) is connected to the fixing plate (22), and the other end of the fixing rod (23) is connected to the sealing plate (24);

the sealing plate (24) is arranged on the side wall of the incubator (1) in a sliding mode, a liquid outlet (11) is formed in the bottom of the side wall of the incubator (1), and the sealing plate (24) is arranged above the liquid outlet (11).

2. A biological cell culture apparatus according to claim 1, wherein a sealing case (12) is provided at the bottom of the incubator (1), the push-pull cylinder (21) is provided in the sealing case (12), an access hole (121) is provided above the sealing case (12), a piston rod of the push-pull cylinder (21) passes through the access hole (121), and the fixing plate (22) is provided above the sealing case (12).

3. A biological cell culture apparatus according to claim 1, wherein the fixing plate (22) is formed by four fixing blocks (221) arranged oppositely, the fixing blocks (221) being independent of each other.

4. A biological cell culture apparatus according to claim 1, wherein a culture dish fixing seat (31) is arranged in the water circulation box (3), a culture dish (4) is mounted on the culture dish fixing seat (31), a drainage tube (41) is connected to the culture dish (4), and the drainage tube (41) is connected to the liquid outlet (11).

5. A biological cell culture apparatus according to claim 1, wherein a thermostat (5) is provided outside the water circulation tank (3), and the thermostat (5) comprises a water tank (51), an operation panel (52), a heating pipe (53), a water pump (54), a water pipe (55) and a second water inlet pipe (56);

the water tank (51) is arranged on one side of the water circulation tank (3), and the water tank (51) is communicated with the water circulation tank (3) through the water conveying pipe (55) and the second water inlet pipe (56);

the operation panel (52) is installed on the side wall of the water tank (51);

the heating pipe (53) is arranged at the bottom of the water tank (51), and the heating pipe (53) is electrically connected with the operation panel (52);

the water pump (54) is arranged at the bottom of the water tank (51), and the water pump (54) is connected with the water conveying pipe (55).