CN218026124U

CN218026124U - Honeycomb culture system

Info

Publication number: CN218026124U
Application number: CN202221972372.7U
Authority: CN
Inventors: 尹果; 翁宽; 谭亮; 宋少龙
Original assignee: Chutian Siweikang Gene Technology Changsha Co ltd
Current assignee: Chutian Siweikang Gene Technology Changsha Co ltd
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2022-12-13
Anticipated expiration: 2032-07-28

Abstract

The utility model discloses a honeycomb culture system, including incubator, control module and a plurality of air supply pipe, be equipped with intake pipe, outlet duct and sensor, each on the incubator the air supply pipe crosses with the intake pipe and is connected, be equipped with parallelly connected low-speed valve and high-speed valve in the intake pipe, be equipped with first check valve on the outlet duct and be connected with the blast pipe, be equipped with discharge valve on the blast pipe, low-speed valve, high-speed valve, discharge valve and sensor all are connected with the control module electricity. This honeycomb culture system has can realize advantages such as quick recovery of the inside microenvironment of incubator and accurate control.

Description

Honeycomb culture system

Technical Field

The utility model relates to a food, medicine mechanical equipment technical field especially relate to a honeycomb cultivation system.

Background

In recent years, cell and gene therapy methods in the field of biotechnology are not only overseas fire and heat, but also in domestic research, and key points such as immune cell therapy, stem cell therapy, gene therapy products, specific immunoglobulin and the like are developed.

A honeycomb cultivation system is an apparatus integrating a plurality of cultivation boxes, each of which is independently operated without the problem of cross contamination. The incubator is widely applied to cell culture and research in life science, such as biological cell, tissue, bacterial culture and the like, and is key equipment for developing immunology, oncology, genetics and bioengineering.

Common honeycomb culture system on the market, to incubator and cell workstation butt joint operation, return honeycomb culture system after, the rapid recovery and the accurate control of its inside microenvironment have provided higher and higher requirement, and current honeycomb culture system can't satisfy the requirement.

In addition, the existing honeycomb cultivation system usually needs to take out the cultivation box from the honeycomb after the production of a sample batch is finished, and sterilize the cultivation box through the isolator after the transportation is docked with a specific isolator, which increases the equipment cost, the transportation workload, the sterilization time and the complexity of system data management.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome prior art not enough, provide a can realize inside microenvironment of incubator quick recovery and accurate control's honeycomb cultivation system.

In order to solve the technical problem, the utility model discloses a following technical scheme:

the utility model provides a honeycomb culture system, includes incubator, control module and a plurality of air supply pipe, be equipped with intake pipe, outlet duct and sensor on the incubator, each the air supply pipe is connected in intersection with the intake pipe, be equipped with parallelly connected low-speed valve and high-speed valve in the intake pipe, be equipped with first check valve on the outlet duct and be connected with the blast pipe, be equipped with discharge valve on the blast pipe, low-speed valve, high-speed valve, discharge valve and sensor all are connected with the control module electricity.

As a further improvement of the above technical solution:

the honeycomb culture system is still including cultivateing the room, the incubator is placed in cultivateing the room, be equipped with first quick-connect joint and second quick-connect joint on the outer wall of cultivateing the room, the one end that the incubator was kept away from to the intake pipe is connected with first quick-connect joint, outlet duct and blast pipe pass through second quick-connect joint and connect, each the air supply pipe is connected with first quick-connect joint intersection.

Still be equipped with first filter in the intake pipe, be equipped with the second filter on the outlet duct.

The air supply pipe is provided with a first buffer tank.

And a first pressure regulating valve, a first ball valve and a first electromagnetic valve which are sequentially arranged along the air flow direction are arranged on the air supply pipe between the first buffer tank and the air inlet pipe.

And the air inlet end of the air supply pipe is provided with a second pressure regulating valve.

The exhaust pipe is further provided with a second buffer tank, a third pressure regulating valve, an air extracting pump and a third filter, and the exhaust valve, the second buffer tank, the third pressure regulating valve, the air extracting pump and the third filter are sequentially arranged along the air flow direction.

The outer wall of the culture chamber is further provided with a third quick connector, a sterilizing tube is connected between the incubator and the third quick connector, the outer end of the third quick connector is connected with a sterilizing gas supply device, and the sterilizing tube is provided with a second one-way valve and a fourth filter.

The sterilizing gas supply device comprises a clean air storage container, a sterilizing solution storage container and an evaporation chamber, wherein the clean air storage container is connected with the evaporation chamber through a first connecting pipe, the sterilizing solution storage container is connected with the evaporation chamber through a second connecting pipe, a third quick joint is connected with the evaporation chamber through a third connecting pipe, a flow regulating valve and a fourth regulating pressure valve are arranged on the first connecting pipe, a peristaltic pump is arranged on the second connecting pipe, and a second ball valve and a second electromagnetic valve are arranged on the third connecting pipe.

The incubator is provided with at least two, and each air supply pipe is connected with the intake pipe of each incubator all in a crossing way.

Compared with the prior art, the utility model has the advantages of:

the utility model discloses a honeycomb culture system, the admission process: the gas medium required by the incubator is supplied through each gas supply pipe, one gas supply pipe supplies one gas medium, and various gas media are converged in the gas supply pipes and can selectively enter the incubator through the low-speed valve or the high-speed valve after being mixed in the gas supply pipes. High-speed valve for CO in incubator ₂ Concentration, O ₂ The concentration and the temperature and the humidity are quickly recovered, and a low-speed valve is used for CO in the incubator ₂ Concentration, O ₂ And (4) accurately controlling the concentration and the temperature and humidity. The problems of rapid recovery and accurate control of the microenvironment in the incubator 1 are solved.

The utility model discloses a honeycomb culture system, during the incubator sterilization, need not to take out the culture room, lets in clean compressed air in the sterilization gas that can obtain toward the evaporation and realizes the online sterilization of incubator. And an air pump is arranged on the exhaust pipe to pump negative pressure and pump out the sterilized gas. The exhaust pipe can be also provided with a sterilizing gas decomposition device, so that the exhausted gas has no environmental pollution.

Drawings

FIG. 1 is a schematic diagram of a first embodiment of the honeycomb cultivation system of the present invention.

FIG. 2 is a schematic structural view of a second embodiment of the honeycomb cultivation system of the present invention.

The reference numerals in the figures denote:

1. an incubator; 11. an air outlet pipe; 111. a first check valve; 112. a second filter; 2. an air inlet pipe; 21. a low speed valve; 22. a high-speed valve; 23. a first filter; 3. a sensor; 4. a gas supply pipe; 41. a first buffer tank; 42. a first pressure regulating valve; 43. a first ball valve; 44. a first solenoid valve; 45. a second pressure regulating valve; 5. a culture chamber; 51. a first quick connect coupling; 52. A second quick connect coupling; 53. a third quick connect coupling; 6. an exhaust pipe; 61. an exhaust valve; 62. a second buffer tank; 63. a third pressure regulating valve; 64. an air pump; 65. a third filter; 7. a sterilizing tube; 71. a second one-way valve; 72. a fourth filter; 8. A sterilizing gas supply device; 81. a clean air storage container; 82. a sterilizing solution storage container; 83. an evaporation chamber; 84. a first connecting pipe; 841. a flow regulating valve; 842. a fourth regulating pressure valve; 85. a second connecting pipe; 851. a peristaltic pump; 86. a third connecting pipe; 861. a second ball valve; 862. a second solenoid valve.

Detailed Description

The invention will be described in further detail with reference to the drawings and specific examples.

As used in this disclosure and in the claims, the terms "a," "an," "the," and/or "the" are not intended to be inclusive in the singular, but rather are inclusive in the plural unless the context clearly dictates otherwise. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Likewise, the word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

Fig. 1 and fig. 2 show the utility model discloses an embodiment of honeycomb culture system, this honeycomb culture system includes incubator 1, control module and a plurality of air supply pipe 4, be equipped with intake pipe 2 on the incubator 1, outlet duct 11 and sensor 3, each air supply pipe 4 crosses with intake pipe 2 and is connected, be equipped with parallelly connected low-speed valve 21 and high-speed valve 22 on the intake pipe 2, be equipped with first check valve 111 on the outlet duct 11 and be connected with blast pipe 6, be equipped with discharge valve 61 on the blast pipe 6, low-speed valve 21, high-speed valve 22, discharge valve 61 and sensor 3 all are connected with the control module electricity.

The gaseous medium required for the normal operation of the incubator 1 comprises nitrogen N ₂ (e.g. 2-8 bar), carbon dioxide CO ₂ (e.g. 2-8 bar), oxygen O ₂ (e.g., 2-8 bar);

and (3) air inlet process: the gas medium required by the incubator 1 is supplied through each gas supply pipe 4, one gas supply pipe 4 supplies one gas medium, and various gas media are converged in the gas supply pipe 2, mixed in the gas supply pipe 2 and then selectively enter the incubator 1 through the low-speed valve 21 or the high-speed valve 22. The high-speed valve 22 is used for CO inside the incubator 1 ₂ Concentration, O ₂ The concentration and the temperature and the humidity are quickly recovered, and the low-speed valve 21 is used for culturing CO in the incubator 1 ₂ Concentration, O ₂ And (4) accurately controlling the concentration and the temperature and humidity. The problems of rapid recovery and accurate control of the microenvironment in the incubator 1 are solved.

Low-speed valve 21, high-speed valve 22, discharge valve 61 are the solenoid valve, and sensor 3 includes gaseous medium depth sensor 3, temperature and humidity sensor 3, and sensor 3 will give control module to the inside detected signal of incubator 1, and control module is according to each valve switch of received detected signal control. This honeycomb culture system can realize recovering fast and accurate control to the inside microenvironment of incubator 1 through the combination of low-speed valve 21 and the parallelly connected setting of high-speed valve 22 and the relevant valve of sensor 3.

In this embodiment, the honeycomb culture system further includes cultivates room 5, and incubator 1 is placed in cultivateing room 5, is equipped with first quick-connect joint 51 and second quick-connect joint 52 on the outer wall of cultivateing room 5, and the one end that incubator 1 was kept away from to intake pipe 2 is connected with first quick-connect joint 51, and outlet duct 11 and blast pipe 6 are connected through second quick-connect joint 52, and each air supply pipe 4 is connected with first quick-connect joint 51 intersection.

In this embodiment, the air inlet pipe 2 is further provided with a first filter 23, and the air outlet pipe 11 is provided with a second filter 112.

In this embodiment, the air supply pipe 4 is provided with a first buffer tank 41.

In this embodiment, the air supply pipe 4 between the first buffer tank 41 and the air inlet pipe 2 is provided with a first pressure regulating valve 42, a first ball valve 43 and a first electromagnetic valve 44 which are sequentially arranged along the air flow direction.

In this embodiment, the air inlet end of the air supply pipe 4 is provided with a second pressure regulating valve 45.

In this embodiment, the exhaust pipe 6 is further provided with a second buffer tank 62, a third pressure regulating valve 63, a suction pump 64, and a third filter 65, and the exhaust valve 61, the second buffer tank 62, the third pressure regulating valve 63, the suction pump 64, and the third filter 65 are sequentially provided in the air flow direction.

In this embodiment, the outer wall of the culture chamber 5 is further provided with a third quick connector 53, a sterilizing tube 7 is connected between the incubator 1 and the third quick connector 53, the outer end of the third quick connector 53 is connected with a sterilizing gas supply device 8, and the sterilizing tube 7 is provided with a second one-way valve 71 and a fourth filter 72.

In this embodiment, the sterilizing gas supply device 8 includes a clean air storage container 81, a sterilizing solution storage container 82 and an evaporation chamber 83, the clean air storage container 81 is connected to the evaporation chamber 83 through a first connection pipe 84, the sterilizing solution storage container 82 is connected to the evaporation chamber 83 through a second connection pipe 85, a third quick connector 53 is connected to the evaporation chamber 83 through a third connection pipe 86, the first connection pipe 84 is provided with a flow control valve 841 and a fourth pressure control valve 842, the second connection pipe 85 is provided with a peristaltic pump 851, and the third connection pipe 86 is provided with a second ball valve 861 and a second solenoid valve 862.

A sterile solution storage tank 82 holds a sterile solution, such as hydrogen peroxide solution. The incubator 1 is provided with a fan.

And (3) sterilization process:

a. the liquid in the sterilizing solution storage container 82 is pumped and conveyed to an evaporation tray in the evaporation chamber 83 through a peristaltic pump 851, a rod heater is installed in the evaporation tray, and when the rod heater heats the evaporation tray to a certain temperature, the sterilizing solution is vaporized and fills the whole evaporation chamber 83;

b. after passing through the flow control valve 841 and the fourth pressure control valve 842, the clean compressed air (for example, 6-8 bar) in the clean air storage container 81 is injected into the evaporation chamber 83, the pressure in the evaporation chamber 83 is increased, so that the vaporized sterilization solution is discharged from the evaporation chamber 83, passes through the second ball valve 861, the second electromagnetic valve 862, the fourth filter 72 and the second one-way valve 71 in sequence, enters the incubator 1, and the blower in the incubator 1 is turned on, thereby achieving sterilization of the interior of the incubator 1.

When the incubator 1 is sterilized, the incubator 1 can be sterilized on line by introducing clean compressed air into the sterilized gas obtained by evaporation without pumping out the incubator 5. The exhaust pipe 6 is provided with a suction pump 64 for sucking a negative pressure to suck the sterilized gas. The exhaust pipe 6 can also be provided with a sterilizing gas decomposition device, so that the exhausted gas has no environmental pollution.

After the sterilization is finished, the peristaltic pump 851 is closed, the sterilization gas is stopped from being added into the evaporation chamber 83, the clean compressed air is continuously introduced, the fan in the incubator 1 is started, the valves on the air outlet pipe 11 and the exhaust pipe 6 and the air suction pump 64 on the exhaust pipe 6 are started, and the gas replacement in the incubator 1 is finished.

In this embodiment, at least two incubators 1 are provided, and each air supply pipe 4 is connected to the air inlet pipe 2 of each incubator 1 in an intersecting manner. The air outlet pipe 11 of each incubator 1 is connected with the exhaust pipe 6 in an intersecting way.

And (3) an exhaust process:

the gas in the incubator 1 sequentially passes through the first one-way valve 111, the second filter 112, the second quick connector 52 and the exhaust valve 61 and then enters the second buffer tank 62; a high-precision and high-stability pressure switch is arranged on the second buffer tank 62, so that the pressure signals of various media can be detected, displayed, alarmed and controlled in real time; the outlet of the second buffer tank 62 is provided with a pressure regulating valve; the gas in the second buffer tank 62 is evacuated to a negative pressure by the air pump 64 and is discharged through the third filter 65. The third filter 65 is a decomposition filter.

Each buffer tank is provided with a high-precision and high-stability pressure switch, so that the pressure signals of various media can be detected, displayed, alarmed and controlled in real time.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention. The technical solution of the present invention can be used by anyone skilled in the art to make many possible variations and modifications, or to modify equivalent embodiments, without departing from the scope of the present invention, using the technical content disclosed above. Therefore, any simple modification, equivalent change and modification made to the above embodiments by the technical entity of the present invention should fall within the protection scope of the technical solution of the present invention.

Claims

1. A honeycomb cultivation system, comprising: including incubator (1), control module and a plurality of air supply pipe (4), be equipped with intake pipe (2), outlet duct (11) and sensor (3), each on incubator (1) air supply pipe (4) are connected in cross with intake pipe (2), be equipped with parallelly connected low-speed valve (21) and high-speed valve (22) in intake pipe (2), be equipped with first check valve (111) on outlet duct (11) and be connected with blast pipe (6), be equipped with discharge valve (61) on blast pipe (6), low-speed valve (21), high-speed valve (22), discharge valve (61) and sensor (3) all are connected with the control module electricity.

2. A bee nest cultivation system according to claim 1, wherein: the honeycomb culture system is still including cultivateing room (5), incubator (1) is placed in cultivateing room (5), be equipped with first quick-connect joint (51) and second quick-connect joint (52) on the outer wall of cultivateing room (5), the one end that incubator (1) was kept away from in intake pipe (2) is connected with first quick-connect joint (51), outlet duct (11) and blast pipe (6) are connected through second quick-connect joint (52), each air supply pipe (4) and first quick-connect joint (51) intersection connection.

3. A bee nest culture system according to claim 2, wherein: still be equipped with first filter (23) on intake pipe (2), be equipped with second filter (112) on outlet duct (11).

4. A bee nest cultivation system according to claim 1, wherein: the gas supply pipe (4) is provided with a first buffer tank (41).

5. A bee nest cultivation system according to claim 4, wherein: and a first pressure regulating valve (42), a first ball valve (43) and a first electromagnetic valve (44) which are sequentially arranged along the airflow direction are arranged on the air supply pipe (4) between the first buffer tank (41) and the air inlet pipe (2).

6. A bee nest cultivation system according to claim 5, wherein: and a second pressure regulating valve (45) is arranged at the air inlet end of the air supply pipe (4).

7. A bee nest culture system according to claim 2, wherein: the exhaust pipe (6) is further provided with a second buffer tank (62), a third pressure regulating valve (63), an air suction pump (64) and a third filter (65), and the exhaust valve (61), the second buffer tank (62), the third pressure regulating valve (63), the air suction pump (64) and the third filter (65) are sequentially arranged along the air flow direction.

8. A bee nest cultivation system according to claim 2, wherein: the outer wall of the culture chamber (5) is further provided with a third quick connector (53), a sterilizing tube (7) is connected between the incubator (1) and the third quick connector (53), the outer end of the third quick connector (53) is connected with a sterilizing gas supply device (8), and the sterilizing tube (7) is provided with a second one-way valve (71) and a fourth filter (72).

9. A bee nest cultivation system according to claim 8, wherein: the sterilizing gas supply device (8) comprises a clean air storage container (81), a sterilizing solution storage container (82) and an evaporation chamber (83), the clean air storage container (81) is connected with the evaporation chamber (83) through a first connecting pipe (84), the sterilizing solution storage container (82) is connected with the evaporation chamber (83) through a second connecting pipe (85), a third quick connector (53) is connected with the evaporation chamber (83) through a third connecting pipe (86), a flow regulating valve (841) and a fourth regulating pressure valve (842) are arranged on the first connecting pipe (84), a peristaltic pump (851) is arranged on the second connecting pipe (85), and a second ball valve (861) and a second electromagnetic valve (862) are arranged on the third connecting pipe (86).

10. A bee nest cultivation system according to any one of claims 1 to 9, wherein: the number of the culture boxes (1) is at least two, and each air supply pipe (4) is connected with the air inlet pipe (2) of each culture box (1) in an intersecting manner.