CN218026113U - Automatic warehouse building instrument for stepped sampling - Google Patents

Abstract

The utility model provides an automatic warehouse building instrument for stepped sampling, which comprises a refrigeration module, a thermal cycle module, a normal temperature reagent storage module, a gun head module, a heating oscillation module, an optical detection module, a magnetic suction module and a mechanical movement module; the gun head module comprises a plurality of gun head frames which can be stacked up and down, a plurality of gun head holes for storing gun heads are arranged on the gun head frames, the gun head frames are provided with positioning connecting mechanisms, and the upper gun head frame and the lower gun head frame are connected through the positioning connecting mechanisms; the mechanical moving module is arranged on the moving guide rail component, the moving guide rail component drives the mechanical moving module to move along the X-axis direction and the Y-axis direction, and the mechanical moving module is provided with a pipettor capable of moving up and down and a manipulator component capable of moving up and down. The utility model discloses can replace the manual work to realize the automatic storehouse operation of building, labour saving and time saving, operating efficiency is high.

Description

Automatic warehouse building instrument for stepped sampling

Technical Field

The utility model relates to the field of biotechnology, in particular to automatic storehouse appearance of building of ladder sample.

Background

The automatic storehouse building instrument is generally used for reagent refrigeration in a second-generation storehouse building, automatic liquid transfer, sample thermal circulation, heating oscillation, magnetic absorption purification and other library preparation works, and has the advantages of high flux, semi-automatic or full-automatic performance, man-machine interaction in intermediate process alternation and the like. The application of the nanopore-based sequencing technology is wider and wider at present, and for acquiring complete nucleic acid information, a manual experiment library construction mode is mainly used for long-chip sequencing library construction, and a scientific research worker needs to repeatedly operate for a long time in the experiment process to obtain a high-quality sequencing library.

The existing main defects are as follows: the existing automatic library building instrument is based on a short-fragment processing automatic working machine platform developed by a second-generation NGS library building scheme, and cannot be used for library building of nanopore single-molecule long-fragment sequencing, so that the library building work of nanopore single-molecule long-fragment sequencing is mainly completed manually, automatic operation cannot be realized, the workload of personnel is large, and the efficiency is low.

Disclosure of Invention

The utility model aims at providing a storehouse appearance is built in automation of ladder sample can simulate manual storehouse mode of building, realizes the automatic of nanopore unimolecule long fragment sequencing library and founds, alleviates personnel's work load, improves the operating efficiency.

The utility model aims at realizing through the following technical scheme: an automatic warehouse building instrument for stepped sampling comprises a refrigeration module, a thermal cycle module, a normal temperature reagent storage module, a gun head module, a heating oscillation module, an optical detection module, a magnetic suction module and a mechanical movement module;

the gun head module comprises a plurality of gun head frames which can be stacked up and down, a plurality of gun head holes for storing gun heads are arranged on the gun head frames, the gun head frames are provided with positioning connecting mechanisms, and the upper gun head frame and the lower gun head frame are connected through the positioning connecting mechanisms;

the mechanical movement module is arranged on the movement guide rail component, the movement guide rail component drives the mechanical movement module to move along the X-axis direction and the Y-axis direction, and a pipettor capable of moving up and down and a manipulator component capable of moving up and down are arranged on the mechanical movement module.

Preferably, the refrigeration module, the thermal cycle module and the normal temperature reagent storage module are arranged on one side of the gun head module, and the heating oscillation module, the magnetic suction module and the optical detection module are arranged on the other side of the gun head module.

Preferably, the magnetic suction module comprises a supporting seat, a PCR plate bracket and a magnetic column bottom plate support, wherein the PCR plate bracket is arranged on the supporting seat, a lifting driving device for driving the magnetic column bottom plate support to move up and down is arranged on the PCR plate bracket, a magnetic column fixing plate is arranged on the magnetic column bottom plate support, and a plurality of magnetic columns are arranged on the magnetic column fixing plate; positioning grooves are arranged on two sides of the upper end of the PCR plate bracket; when the PCR plate is placed on the PCR plate bracket, the two sides of the PCR plate are clamped into the positioning grooves on the PCR plate bracket, and the magnetic column fixing plate is positioned below the PCR plate.

Preferably, the positioning connection mechanism comprises a positioning sleeve arranged on the upper side of the gun head frame and a positioning insertion rod arranged below the gun head frame and corresponding to the positioning sleeve, and when the gun head frames are vertically stacked, the positioning insertion rod on the gun head frame above is inserted into the positioning sleeve on the gun head frame below.

Preferably, the mechanical moving module comprises a mounting base plate, the mounting base plate is connected with the moving guide rail part, a lifting frame is connected onto the mounting base plate in a sliding manner, and a lifting driving mechanism for driving the lifting frame to move is arranged on the mounting base plate; the pipettor and the manipulator component are arranged on the lifting frame, and the lifting frame is also provided with a pipettor lifting driving device for driving the pipettor to move up and down and a manipulator component lifting driving device for driving the manipulator component to move up and down.

Preferably, the lifting driving mechanism comprises a lifting driving motor and a first guide rail, the lifting driving motor is arranged on the mounting base plate, the first guide rail is vertically arranged on the mounting base plate, a first sliding block is connected to the first guide rail, and the lifting frame is arranged on the first sliding block; the mounting base plate is rotatably connected with a screw rod, the screw rod is parallel to the first guide rail and is in transmission connection with a lifting driving motor, a threaded hole corresponding to the screw rod is formed in the lifting frame, and the screw rod is in threaded connection with the threaded hole.

Preferably, the lifting frame is provided with a fixed plate, the fixed plate is provided with a second guide rail, the second guide rail is arranged along the vertical direction, the second guide rail is connected with a second sliding block, and the manipulator part is arranged on the second sliding block; but the manipulator part lift drive is equipped with vertical mobile's first output shaft on, and the manipulator part is connected to the one end of first output shaft.

Preferably, the pipettor lifting driving device is provided with a second output shaft capable of vertically moving, and one end of the second output shaft is connected with the pipettor.

The utility model has the advantages that: the utility model discloses can replace the manual work to realize the automatic storehouse operation of building, labour saving and time saving, operating efficiency is high.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a top view of the present invention.

Fig. 3 is a schematic structural view of the lance tip module.

Fig. 4 is a front view of the mechanical movement module.

Fig. 5 is a side view of the mechanical movement module.

Fig. 6 is a schematic view of the mechanical movement module mounting eight pipette tips.

Fig. 7 is a schematic view of the mechanical movement module mounting a single pipette tip.

FIG. 8 is a schematic view of the mechanical movement module grasping the PCR plate.

FIG. 9 is a schematic diagram of the mechanical movement module grasping the nucleic acid quantifying turntable.

Fig. 10 is a schematic diagram of the mechanical movement module grasping the headstock.

Fig. 11 is a schematic structural view of a heating oscillation module.

FIG. 12 is a schematic view of a magnetic module.

FIG. 13 is a front view of the magnetic attraction module.

FIG. 14 is a schematic diagram of one of the structures of the PCR plate.

FIG. 15 is another schematic diagram of the structure of the PCR plate.

FIG. 16 is a schematic view of a PCR plate placed on a magnetic module and a magnetic column near the PCR plate.

FIG. 17 is a schematic view of the PCR plate placed on the magnetic module and the magnetic columns away from the PCR plate.

FIG. 18 is a schematic view of an optical inspection module.

In the figure: 1. y-axis moving guide rails, 2X-axis moving guide rails, 3 mechanical moving modules, 4 refrigeration modules, 5 normal temperature reagent storage modules, 6 thermal cycle modules, 8 gun heads, 11 heating oscillation modules, 12 magnetic attraction modules, 13 optical detection modules, 14 waste consumable storage frames, 15 racks, 16 gun head frames, 17 positioning insertion rods, 18 positioning sleeves, 19 positioning sleeves, bases, 20 gun head holes, 21 mounting base plates, 22 first guide rails, 23 first sliding blocks, 24 lifting driving motors, 25 screw rods, 26 lifting frames, 27 pipettors, 28, a pipettor lifting drive device, 29, a gun head, 30, a manipulator component, 31, a fixing plate, 32, a second guide rail, 33, a second slide block, 34, a manipulator component lifting drive device, 35, a PCR plate, 36, a nucleic acid quantitative turntable, 40, a PCR plate heat conduction frame, 41, a bottom plate, 42, a support column, 43, a PCR plate bracket, 44, a linear motor, 45, a positioning groove, 46, a guide rod, 47, a sliding sleeve, 48, a magnetic column bottom plate bracket, 49, a magnetic column fixing plate, 50, a magnetic column, 52, a grabbing part, 53, a nucleic acid quantitative reagent storage frame, 54 and a quantitative turntable temporary suspension frame.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.

It will be understood by those skilled in the art that in the present disclosure, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in a generic and descriptive sense only and not for purposes of limitation, as the terms are used in the description to indicate that the referenced device or element must have the specified orientation, be constructed and operated in the specified orientation, and not for the purposes of limitation.

It is understood that the terms "a" and "an" should be interpreted as meaning that a number of one element or element is one in one embodiment, while a number of other elements is one in another embodiment, and the terms "a" and "an" should not be interpreted as limiting the number.

As shown in fig. 1 to 18, an automatic warehouse building instrument for step sampling comprises a rack 15, a refrigeration module 4, a thermal cycle module 6, a normal temperature reagent storage module 5, a gun head module 8, a heating oscillation module 11, an optical detection module 13, a magnetic suction module 12, a mechanical movement module 3, and a waste consumable storage frame 14. Refrigeration module 4, thermal cycle module 6, normal atmospheric temperature reagent storage module 5 are the inline and set up in one side of rifle head module 8, and the heating is shaken module 11, is inhaled module 12 and optical detection module 13 and abandonment consumptive material and is deposited frame 14 and be located the opposite side of rifle head module 8.

The gun head module 8 comprises a plurality of gun head frames 16 which can be stacked up and down, and a plurality of gun head holes 20 for storing gun heads 29 are formed in the gun head frames 16. The gun head frame is provided with a positioning connecting mechanism, and the upper gun head frame and the lower gun head frame are connected through the positioning connecting mechanism. In this embodiment, each gun head frame 16 is provided with 96 gun head holes 20, and the gun head holes 20 are arranged on the gun head frame 16 in an 8-by-12 manner. The gun heads 29 are inserted into the gun head holes 20 on the gun head frames 16, and each gun head frame 16 can accommodate 96 gun heads 29.

The headstock 8 is provided with a positioning connection mechanism, and when the headstock 8 is vertically stacked, the upper and lower headstock 6 are connected through the positioning connection mechanism. Specifically, the headstock 8 is provided with a positioning inserted bar 17 and a positioning sleeve 18 corresponding to the positioning inserted bar 17, and the positioning inserted bar 17 and the positioning sleeve 18 are respectively arranged on the upper side and the lower side of the headstock 8. In the application, each gun head frame 16 is provided with four positioning insertion rods 17 and four positioning sleeves 18, wherein the positioning sleeves 18 are arranged on the upper side of the gun head frame 16 and are positioned on the periphery of the gun head frame 16; the positioning plunger 17 is located on the underside of the headstock 16 and around the headstock 16. When the gun head frame 16 is vertically stacked, the positioning inserted rod 17 on the upper gun head frame 16 is inserted into the positioning sleeve 18 on the lower gun head frame 16, so that the gun head frame is stably stacked, and the gun head frame is prevented from overturning. A plurality of gun head frames 16 are vertically stacked to form a vertically stacked group, and the gun head storage module can be composed of one or more vertically stacked groups.

Further, the gun head storage module further comprises a base 19, a placing groove is formed in the base 19, the placing groove is matched with the shape of the gun head frame 16, the gun head frame 16 located at the lowest position is placed on the base 19, and the gun head frame 16 is embedded into the placing groove in the base 19, so that stable placement of the whole vertical stacking group is guaranteed.

The mechanical movement module 3 is provided on a movement guide member, the movement guide member drives the mechanical movement module 3 to move in the X-axis direction and the Y-axis direction, and the mechanical movement module 3 is provided with a pipettor 27 capable of moving up and down and a manipulator member 30 capable of moving up and down.

The movable guide rail component comprises an X-axis movable guide rail 2 and a Y-axis movable guide rail 1, the Y-axis movable guide rail 1 is arranged on the X-axis movable guide rail 2, and the mechanical movable module 3 is arranged on the Y-axis movable guide rail 1. The movement of the mechanical movement module in the X-axis direction and the Y-axis direction is realized through the X-axis movement guide rail and the Y-axis movement guide rail 2.

Specifically, the mechanical movement module 3 includes a mounting substrate 21, the mounting substrate 21 is connected to the movable guide rail part, a lifting frame 26 is slidably connected to the mounting substrate 21, and a lifting driving mechanism for driving the lifting frame to move is provided on the mounting substrate 21. The pipettor 27 and the manipulator unit 30 are provided on the crane 26, and the crane 26 is further provided with a pipettor elevation driving device 28 for driving the pipettor 27 to move up and down and a manipulator unit elevation driving device 34 for driving the manipulator unit 30 to move up and down.

Wherein, lift actuating mechanism is including setting up lift driving motor 24, the first guide rail 22 on mounting substrate 21, and first guide rail 22 is vertical to be set up on mounting substrate 21, is connected with first slider 23 on the first guide rail 22, first slider 23 and first guide rail 22 sliding connection, and crane 26 installs on first slider 23, and crane 26 can be along first guide rail 22 vertical migration. The mounting base plate 21 is rotatably connected with a screw rod 25, the screw rod 25 is parallel to the first guide rail 22, the screw rod 25 is in transmission connection with a lifting driving motor 24, a threaded hole corresponding to the screw rod 25 is formed in the lifting frame 26, and the screw rod 25 is in threaded connection with the threaded hole.

In this embodiment, the lifting driving motor 24 is connected to the screw rod 25 through a belt. Wherein, the output shaft of the lifting driving motor 24 and one end of the screw rod 25 are respectively provided with a belt pulley, a belt is connected between the two belt pulleys, and the transmission between the lifting driving motor and the screw rod is realized through the belt. The lifting driving motor and the screw rod can also adopt gear connection or other transmission modes. The lead screw is driven to rotate by the lifting driving motor, and the lifting frame is driven to move up and down when the lead screw rotates.

The lifting frame 26 is provided with a fixing block 31, the fixing block 31 is provided with a second guide rail 32, the second guide rail 32 is arranged along the vertical direction, the second guide rail 32 is connected with a second sliding block 33, and the manipulator part 30 is arranged on the second sliding block 33. The robot member elevation driving device 34 is provided with a first output shaft that is vertically movable, and one end of the first output shaft is connected to the robot member 30. In this embodiment, the robot member elevation driving device 34 is an electric push rod. The robot member 30 moves up and down by the robot member elevation drive device 34. The robot part 30 is provided with two jaws which can be opened or closed. As shown in FIGS. 8 to 10, the robot part can grasp the PCR plate 35, the nucleic acid quantitative turntable 36 and the gun head rack 16, and the above-mentioned devices can be transported by the mechanical moving module. Wherein, the nucleic acid quantitative rotating disc 36 is provided with a grabbing part 52 for facilitating the grabbing of the nucleic acid quantitative rotating disc 36 by the manipulator part 30; when the robot member 30 grips the nucleic acid quantifying turntable 36, the gripping claws grip both sides of the gripping section 52. When the PCR plate 35 is gripped, the grip jaws of the robot part 30 grip both ends of the PCR plate 35. When the gun head holder 16 is grasped, the two jaws of the robot part 30 grip both ends of the gun head holder 16.

Further, the inner side of the clamping jaw is provided with a clamping groove 35, when the device is used for grabbing, the grabbing part 52 or the PCR plate 35 or two ends of the gun head frame 16 are clamped into the clamping groove, so that the device is stably grabbed.

The pipettor lifting drive device 28 is provided with a second output shaft capable of moving vertically, and one end of the second output shaft is connected with the pipettor 27. In this embodiment, the pipette lift driving device 28 is an electric push rod. Preferably, the pipette 27 is an eight-channel pipette, and a maximum of eight tips 29 can be connected to the pipette 27, so that eight liquid samples can be simultaneously pipetted.

Be equipped with the deep hole board on the normal atmospheric temperature reagent storage module 5, be equipped with a plurality of deep hole that is used for depositing normal atmospheric temperature reagent on the deep hole board, normal atmospheric temperature reagent is stored in each deep hole on the deep hole board. The pipettor may aspirate the desired ambient temperature reagents from the deep well plate. The normal temperature reagent comprises the following components: magnetic beads, nucleic acid eluents, and the like.

The refrigeration module 4 is prior art and is used for storing some low temperature reagents and for dispensing the reagents under low temperature conditions.

The heating and vibrating module 11 is used for heating and vibrating the detection sample. The heating oscillation module 11 is provided with a PCR plate heat conduction frame 40. The PCR plate heat conduction frame 40 is provided with holes matched with the PCR plate. When using, place the PCR board on PCR board heat conduction frame 40, the module 11 is vibrate and the heating is shaken to the PCR board to vibrate the process of hatching. Wherein, the module 11 adopts prior art is vibrated in the heating, and the concrete structure and the principle that the module 11 was vibrated in the heating can be referred to the utility model patent that the publication is CN209865901U, and this utility model patent's name is a vibration that has heating and refrigeration function shakes even device.

The magnetic module 12 includes a support base, a PCR plate bracket 45 disposed on the support base, and a magnetic pillar base plate bracket 48. Wherein, the support base comprises a bottom plate 41 and a support column 42 arranged on the bottom plate 41, and a PCR plate bracket 45 is fixed on the upper end of the support column 42. The PCR plate bracket 45 is U-shaped, and the PCR plate bracket 45 is provided with a lifting driving device 44 for driving the magnetic column bottom plate bracket 48 to move up and down. Wherein, the lifting driving device 44 is arranged at the lower end of the PCR plate bracket 45, the lifting driving device 44 is vertically arranged, the lifting driving device 44 is provided with an output shaft which can move up and down, and the magnetic column bottom plate bracket 48 is fixedly arranged at the upper end of the output shaft of the lifting driving device 44. In this embodiment, the lifting driving device 44 is a linear push rod motor. The magnetic column bottom plate support 48 is provided with a magnetic column fixing plate 49, and the magnetic column fixing plate 49 is provided with a plurality of magnetic columns 50. Magnetic columns 50 are arranged in a rectangular array on magnetic column mounting plate 49. Positioning grooves are arranged on two sides of the upper end of the PCR plate bracket 45. When the PCR plate 35 is placed on the PCR plate holder 45, both sides of the PCR plate 35 are caught in the positioning grooves on the PCR plate holder 45, and the magnetic column fixing plate is positioned below the PCR plate. As shown in fig. 14 and 15, current PCR board has two kinds of structures usually, including half skirt border PCR board and full skirtboard PCR board, the utility model provides a constant head tank on the module is inhaled to magnetism, can be applicable to half skirt border PCR board and full skirtboard PCR board simultaneously, when using, the both sides homoenergetic of half skirt border PCR board and full skirtboard PCR board and the constant head tank looks adaptation of inhaling on the module of magnetism, half skirt border PCR board and full skirtboard PCR board homoenergetic fall into in the constant head tank.

After the PCR plate with the detection sample and the magnetic beads is placed on the PCR plate bracket, the distance between the magnetic columns and the PCR plate can be changed by the lifting driving device 44; carry out high-order magnetism when the purification and inhale, ensure to detect liquid phase separation in the sample for reduce the loss of sample when siphoning away the waste liquid, can reduce the low level magnetic pole again when the elution, guarantee to pass through all magnetic beads with minimum eluant, reduce the experiment system, can reduce the reagent quantity of later stage experiment simultaneously, make the experiment cost reduce by a wide margin. Wherein, PCR plate bracket can be placed with the compatibility of half shirt rim PCR board and full skirtboard PCR on the current market, and the influence that the difference in height brought can be eliminated to the magnetic column of deuterogamy to guarantee the uniformity of experimental result.

As shown in fig. 18, the optical detection module 13 of the present invention adopts the prior art, and the optical detection module is used for performing optical detection on the detection sample. Wherein, the optical detection module 13 is provided with a nucleic acid quantitative reagent storage rack 53. When detecting, the detection sample is firstly placed on the nucleic acid quantitative rotating disc 36, then the nucleic acid quantitative rotating disc 36 with the detection sample is placed at the corresponding position on the optical detection module 13 through the mechanical movement module 3, and the optical detection is performed on the detection sample. After the detection, the turntable 36 can be moved away by the mechanical moving module 3, and then the next batch is replaced for detection.

Further, a quantitative turntable temporary support 54 is further disposed beside the optical detection module 13, wherein two placing positions for placing the nucleic acid quantitative turntable 36 are disposed on the quantitative turntable temporary support 54. In practical use, the nucleic acid quantitative rotary disk 36 with the detection sample can be temporarily placed on the quantitative rotary disk temporary holding frame 54 for queue detection, and after the detection sample on the nucleic acid quantitative rotary disk 36 on the optical detection module 13 is completely finished, the nucleic acid quantitative rotary disk on the quantitative rotary disk temporary holding frame 54 is transferred to the optical detection module 13 for detection through the mechanical movement module.

The utility model discloses in be equipped with 96 hole sites on used PCR board 35, for 96 hole boards to 8 set up by 12's mode.

The utility model discloses in, mechanical movement module 3 can replace the manual work to shift to detecting sample and reagent, can carry PCR board 35, nucleic acid ration carousel 36 and rifle head frame 16 simultaneously and carry. Because the pipettor need constantly change rifle head 29 at the in-process that shifts detection sample and reagent, consequently the utility model discloses in be equipped with rifle head module 8, the rifle head 29 that has certain quantity is stored on the rifle head module 8, supplies the pipettor to change. Wherein, rifle head module 8 is three-dimensional storage mode, compares the storage mode of traditional rifle head frame individual layer tiling, under the prerequisite of the same quantity rifle head of storage, the utility model discloses the tiling area that occupies is littleer, arranges compacter, has reduced the area of automatic storehouse appearance of building. And due to the reduction of the tiling area of the gun head frame, the stroke span of the pipette in the horizontal direction is reduced when the pipette head is taken, so that the arrangement length of the guide rail can be shortened, and the cost of equipment is reduced.

The utility model discloses in, the rifle head frame is vertical stacks, and when taking the rifle head, the height drop between the rifle head frame of top and the rifle head frame of below is great, consequently when taking the rifle head, needs the pipettor to have great displacement stroke in vertical direction. In the utility model, the lifting frame moves up and down under the driving of the lifting driving mechanism, and the lifting frame drives the pipettor and the mechanical clamping jaw to realize one-level lifting when moving up and down; the pipettor lifting driving device can drive the pipettor to move up and down, so that the pipettor can be lifted up and down on the basis of one-level lifting, the lifting stroke of the pipettor is effectively improved, and the taking of the gun heads at different height positions is met. Similarly, the manipulator part lifting driving device can drive the manipulator part to move up and down, so that the manipulator part can be lifted up and down at two levels on the basis of lifting at one level, the moving stroke of the manipulator part in the vertical direction is effectively improved, and the carrying requirements on the gun head frames with different heights are met.

The method for taking the gun heads comprises the following steps:

1) Establishing a gun head storage module: the gun head is placed in a gun head hole in the gun head frame, then the gun head frames are sequentially stacked from bottom to top to form a vertical stacking group, then the vertical stacking groups are sequentially arranged from front to back along the horizontal direction, and the gun head frames on the top of the vertical stacking groups are located at the same height.

2) Taking a gun head: taking a gun head from a gun head frame on the uppermost layer; when the pipette is taken, the mechanical moving module is driven to reach the position above the taking position through the moving guide rail part, then the pipettor descends, a butt joint port of the pipette head is in butt joint with the pipette head on the pipette head frame, the pipette head is connected to the pipettor, and then the pipettor ascends, so that the taking of the pipette head is completed; when all the gun heads on the gun head frame are used up, the gun head frame is grabbed and moved out through a manipulator part on the mechanical moving module, and then the gun heads are used on the gun head frames at the top of the other vertical stacking groups in the same way; and when all the gun head frames on the uppermost layer are used up, returning the mechanical moving module to the rearmost vertical stacking group, taking the gun heads from the gun head frames on the second layer in the same way, and so on until all the gun heads are taken up.

The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by the teaching of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as the present invention, fall within the protection scope of the present invention.

Claims (8)

1. An automatic warehouse building instrument for stepped sampling is characterized by comprising a refrigeration module, a thermal cycle module, a normal temperature reagent storage module, a gun head module, a heating oscillation module, an optical detection module, a magnetic suction module and a mechanical moving module;

the gun head module comprises a plurality of gun head frames which can be stacked up and down, a plurality of gun head holes for storing gun heads are arranged on the gun head frames, the gun head frames are provided with positioning connecting mechanisms, and the upper gun head frame and the lower gun head frame are connected through the positioning connecting mechanisms;

the mechanical movement module is arranged on the movement guide rail component, the movement guide rail component drives the mechanical movement module to move along the X-axis direction and the Y-axis direction, and a pipettor capable of moving up and down and a manipulator component capable of moving up and down are arranged on the mechanical movement module.

2. The automatic warehouse building instrument for stepped sampling according to claim 1, wherein the refrigeration module, the thermal cycle module and the normal temperature reagent storage module are arranged on one side of the gun head module, and the heating oscillation module, the magnetic absorption module and the optical detection module are arranged on the other side of the gun head module.

3. The automatic warehouse building instrument for stepped sampling according to claim 1, wherein the magnetic attraction module comprises a support base, a PCR plate bracket and a magnetic column base plate bracket, the PCR plate bracket is arranged on the support base, the PCR plate bracket is provided with a lifting driving device for driving the magnetic column base plate bracket to move up and down, the magnetic column base plate bracket is provided with a magnetic column fixing plate, and the magnetic column fixing plate is provided with a plurality of magnetic columns; positioning grooves are arranged on two sides of the upper end of the PCR plate bracket; when the PCR plate is placed on the PCR plate bracket, the two sides of the PCR plate are clamped into the positioning grooves on the PCR plate bracket, and the magnetic column fixing plate is positioned below the PCR plate.

4. The automatic warehouse building instrument for the stepped sampling according to claim 1, wherein the positioning connection mechanism comprises a positioning sleeve arranged on the upper side of the gun head frame and a positioning insertion rod arranged below the gun head frame and corresponding to the positioning sleeve, and when the gun head frames are vertically stacked, the positioning insertion rod on the upper gun head frame is inserted into the positioning sleeve on the lower gun head frame.

5. The automatic warehouse building instrument for the step sampling according to claim 1, wherein the mechanical moving module comprises a mounting base plate, the mounting base plate is connected with the moving guide rail part, a lifting frame is connected on the mounting base plate in a sliding manner, and a lifting driving mechanism for driving the lifting frame to move is arranged on the mounting base plate; the pipettor and the manipulator component are arranged on the lifting frame, and the lifting frame is also provided with a pipettor lifting driving device for driving the pipettor to move up and down and a manipulator component lifting driving device for driving the manipulator component to move up and down.

6. The automatic warehouse building instrument for the stepped sampling according to claim 5, wherein the lifting driving mechanism comprises a lifting driving motor and a first guide rail which are arranged on the mounting base plate, the first guide rail is vertically arranged on the mounting base plate, a first sliding block is connected to the first guide rail, and the lifting frame is arranged on the first sliding block; the mounting base plate is rotatably connected with a screw rod, the screw rod is parallel to the first guide rail and is in transmission connection with a lifting driving motor, a threaded hole corresponding to the screw rod is formed in the lifting frame, and the screw rod is in threaded connection with the threaded hole.

7. The automatic warehouse building instrument for the stepped sampling according to claim 5, wherein the lifting frame is provided with a fixed plate, the fixed plate is provided with a second guide rail, the second guide rail is arranged along the vertical direction, a second sliding block is connected to the second guide rail, and the manipulator part is installed on the second sliding block; but the manipulator part lift drive is equipped with vertical mobile's first output shaft on, and the manipulator part is connected to the one end of first output shaft.

8. The automatic warehouse building instrument for the step sampling according to claim 1, wherein the pipettor lifting drive device is provided with a second output shaft capable of moving vertically, and one end of the second output shaft is connected with the pipettor.

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