CN115786070A - Semi-automatic nucleic acid detection equipment - Google Patents

Abstract

The invention provides semi-automatic nucleic acid detection equipment which comprises a rack, wherein a working platform is arranged on the rack, and a cover opening module, a liquid transfer module, a clamping and transferring module, a nucleic acid extraction module and a sample application module are arranged on the rack; the clamping module is arranged on the working platform; the uncapping module clamps the sample tube and moves to the clamping module for clamping the tube body of the sample tube, and the uncapping module can clamp the upper cover of the sample tube and rotate; the pipetting module extracts a first preset amount of sample liquid from the sample tube with the upper cover opened at the clamping module and moves the sample liquid into a deep hole plate on the working platform; the clamping and transferring module is configured to clamp the deep hole plate to the nucleic acid extraction and placement position; the nucleic acid extraction module is configured to extract nucleic acids of the sample liquid within the deep well plate at a nucleic acid extraction site; the clamping and transferring module moves the deep-hole plate after the nucleic acid is extracted to the sample application module, and the sample application module extracts a second preset amount of nucleic acid extracting solution in the deep-hole plate to the sample application plate to form a nucleic acid sample to be detected.

Description

Semi-automatic nucleic acid detection equipment

Technical Field

The invention belongs to the technical field of automatic nucleic acid detection, and particularly relates to semi-automatic nucleic acid detection equipment.

Background

The working process of nucleic acid detection generally comprises the steps of opening a sample tube, then extracting a certain amount of liquid in the sample tube, moving the liquid into a deep-hole plate, then extracting nucleic acid, after the nucleic acid extraction is finished, extracting a certain amount of nucleic acid extracting solution, carrying out sample application to obtain a nucleic acid sample, and then entering a QPCR detection instrument for amplification detection.

Present equipment of current nucleic acid detection of using such as laboratory mostly is after the single equipment concatenation with above-mentioned each process of realization, forms whole production line, and above-mentioned nucleic acid detection production line area is big, and the equipment back, shifts once more and need dismantle just can change the work place, and time consuming is long.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide semi-automatic nucleic acid detection equipment, which integrates the structures of all parts to form integral equipment, is convenient to transfer quickly, occupies small area and saves working space.

In order to achieve the purpose, the invention adopts the following technical scheme: the present invention provides a semi-automatic nucleic acid detecting apparatus, comprising: the device comprises a rack, a sample application module, a clamping and transferring module, a nucleic acid extracting module and a sample application module, wherein a working platform is arranged on the rack, and a cover opening module, a liquid transferring module, a clamping and transferring module, a nucleic acid extracting module and a sample application module which can all move back and forth relative to the rack along the X direction, the Y direction and the Z direction are arranged on the rack; the clamping module is arranged on the working platform and is positioned between the uncovering module and the pipetting module; the uncapping module clamps a sample tube positioned on the working platform and moves to a clamping module, the clamping module is configured to clamp a tube body of the sample tube, and the uncapping module can clamp an upper cover of the sample tube and rotate to open or screw the upper cover; the pipetting module extracts a first preset amount of sample liquid from the sample tube with the upper cover opened at the clamping module and moves the sample liquid into a deep hole plate on the working platform; the clamping and transferring module is arranged on one side of the liquid transferring module and is configured to clamp the deep hole plate to a nucleic acid extracting and placing position which is arranged on the working platform and is positioned at the nucleic acid extracting module; the nucleic acid extraction module is arranged at the rear side of the clamping and transferring module and the sample application module, and is configured to extract nucleic acid of the sample liquid in the deep-hole plate at the position of the nucleic acid extraction position; the clamping and transferring module moves the deep-hole plate after nucleic acid extraction to the sample application module, and the sample application module extracts a second preset amount of nucleic acid extracting solution in the deep-hole plate to the sample application plate to form a nucleic acid sample to be detected.

Compared with the prior art, the invention has the following beneficial effects: in the invention, all the modules are arranged on the same rack, so that the semi-automatic equipment is formed, consumables are supplemented by manual feeding, and nucleic acid extraction is automatically completed for detection. All modules are sequentially and compactly arranged, the structure volume is small, and the occupied area is reduced. In addition, the device as a whole structure can be transferred integrally, put into use quickly and start to work. Because of the above-mentioned each group's deep hole board that is used for extracting nucleic acid and is used for placing various consumptive materials on nucleic acid extraction consumptive material position sets up along X to side by side, during nucleic acid extraction, nucleic acid extraction module during operation is along X to removing, uses various consumptive materials in above-mentioned each deep hole board, carries out nucleic acid extraction to nucleic acid extraction the sample liquid in the deep hole board of placing position department. The arrangement mode of each consumable article is matched with the nucleic acid extraction module to move along the X direction, so that the nucleic acid extraction can be rapidly started to extract the nucleic acid from the sample liquid in the deep hole plate at the nucleic acid extraction placing position, and the working efficiency is improved. Meanwhile, the nucleic acid extraction module and the consumable parts are positioned at the rear side of the sample application module, the nucleic acid extraction module for completing the nucleic acid extraction process and the nucleic acid extraction module for completing the sample application process are arranged in front and at the back in the same space, the occupied space in the X direction is saved, the overall structure is more compact, the volume of the whole machine is reduced, the single machine works in a single mode, and the transfer is convenient.

Drawings

FIG. 1 is a schematic view of a semi-automatic nucleic acid detecting apparatus according to the present invention; fig. 2 is a schematic structural view of the door opening module in the present invention; FIG. 3 is a schematic structural view of the uncapped Y-direction loading assembly, the sample rack and the sample tubes in the present invention; FIG. 4 is a schematic structural view of a cover opening Y-direction feeding assembly in the invention; fig. 5 is a schematic structural view of the cover opening Z-direction moving assembly, the grabbing assembly and the buffering assembly in the invention; FIG. 6 is a schematic view of the construction of the lid opening Z-direction moving assembly and the buffer assembly according to the present invention; FIG. 7 is a schematic structural diagram of two sets of clamping modules according to the present invention; FIG. 8a is a schematic view of a first angle configuration of a clamping module of the present invention; FIG. 8b is a second angle schematic view of the clamping module of the present invention; FIG. 9 is a schematic structural view of a pipetting module according to the invention; FIG. 10 is a schematic view of the pipetting assembly of the present invention; FIG. 11 is a schematic view of the structure of a transfer clamping module and a spotting module in the present invention; FIG. 12 is a schematic view of a portion of the transfer clamp assembly and a second code scanning module of the present invention; FIG. 13a is a schematic view of the clamp arm of the present invention; FIG. 13b is a schematic view of the clamp arm of the present invention (excluding the clamp arm body); FIG. 14 is a schematic view of the spotting platform assembly and the configuration of each deep-well plate placement site at a first angle in the present invention; FIG. 15 is a schematic view of the spotting platform assembly and various deep-well plate placement positions at a second angle in the present invention; FIG. 16 is a schematic diagram of a nucleic acid extraction module according to the present invention at a first angle; FIG. 17 is a schematic diagram of a nucleic acid extraction module according to the present invention from a second perspective; FIG. 18 is a schematic view of the nucleic acid extraction installation set of the present invention at a first angle; FIG. 19 is a schematic view of the nucleic acid extraction installation set of the present invention from a second angle; FIG. 20 is a schematic view of the nucleic acid extraction installation set of the present invention at a third angle.

Detailed Description

The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention and are not to be construed as limiting the present invention.

As shown in fig. 1 to 20, the present embodiment provides a semi-automatic nucleic acid detecting apparatus, which includes a rack 1, a cover opening module 2, a clamping module 3, a pipetting module 4, a clamping and transferring module 6, a nucleic acid extracting module 7, and a spotting module 9, wherein a working platform 100 is provided on the rack 1, and the cover opening module 2 is provided on the rack 1 and can move back and forth in X, Y, and Z directions relative to the rack 1. The clamping module 3 is arranged on the working platform 100 and is located on one side of the uncovering module 2, the uncovering module 2 clamps the sample tube 300 located on the working platform 100 and moves to the clamping module 3, the clamping module 3 is configured to clamp the sample tube 300, and the uncovering module 2 can clamp the upper cover of the sample tube 300 and rotate to open or screw. The pipetting module 4 is arranged on the rack 1 and is movable in the X, Y and Z directions relative to the rack 1. The pipetting module 4 withdraws a first predetermined amount of sample liquid from the sample tube 300 with the upper lid open into the deep well plate located on the work platform 100. The clamping module 3 is arranged between the uncovering module 2 and the pipetting module 4, the uncovering module 2, the clamping module 3 and the pipetting module 4 are sequentially arranged along the X direction, and the space between the uncovering module 2 and the pipetting module 4 is fully utilized to reduce the volume of the whole structure. Simultaneously, the three modules are sequentially arranged along the X direction, so that the clamping, uncovering and liquid transferring actions on the sample tube 300 can be completed at the highest speed, and the working efficiency is improved.

The clamping and transferring module 6 is arranged on the rack 1 and is positioned at one side of the liquid transferring module 4, and the clamping and transferring module 6 can move along the X direction, the Y direction and the Z direction relative to the rack 1. The clamping and transferring module 6 can clamp the deep well plate at the pipetting module 4 to the nucleic acid extraction and placement position 705, and the nucleic acid extraction and placement position 705 is arranged on the working platform 100 and is positioned at the nucleic acid extraction module 7. The nucleic acid extraction module 7 is provided on the rack 1 and is movable in the X, Y, and Z directions with respect to the rack 1, the nucleic acid extraction module 7 being configured to use consumables to extract nucleic acids of the sample liquid within the deep well plate at the nucleic acid extraction location 705. The deposition module 8 is disposed on the frame 1 and is movable in the X, Y, and Z directions with respect to the frame 1. The clamping and transferring module 6 moves the deep-well plate after extracting nucleic acid to the spotting plate placing position 801 of the spotting module 8, and the spotting module 8 extracts a second predetermined amount of nucleic acid extracting solution to the spotting plate to form a nucleic acid sample to be detected. The sample spotting plate placing position 801 is arranged on the front side of the nucleic acid extraction consumable position and is arranged in parallel, and the clamping and transferring module 6 and the sample spotting module 8 synchronously move along the X axis.

The clamping and transferring module 6 clamps the deep-well plate at the pipetting module 4 and moves to the nucleic acid extraction and placement position 705, the nucleic acid extraction module 7 moves to the nucleic acid extraction and placement position 705, nucleic acid extraction is carried out on sample liquid in the deep-well plate, after nucleic acid is extracted, the clamping and transferring module 6 moves the deep-well plate to the sample plate placement position 801, and the sample application module 8 extracts a second predetermined amount of nucleic acid extracting solution to the sample plate to form a nucleic acid sample to be detected. Press from both sides and get transfer module 6 and press from both sides the deep hole board that nucleic acid carried set position 705 and place the position 801 to the point model when pressing from both sides, press from both sides transfer module 6 and point sample module 8 along X to synchronous motion, press from both sides transfer module 6 and press from both sides this deep hole board and get in place the back, point sample module 8 readjusts along X to, Y to and Z to the position, reduce point sample module 8's adjustment time for point sample module 8 puts in the time that set position 801 was placed to the point model, improve work efficiency.

In this embodiment, because above-mentioned each module all sets up on same frame 1, becomes semi-automatic equipment, and artifical material loading supplements the consumptive material, accomplishes nucleic acid extraction voluntarily to be ready for the detection and use. All modules are sequentially and compactly arranged, the structure volume is small, and the occupied area is reduced. In addition, the device as a whole structure can be transferred integrally, put into use quickly and start to work. Because the deep-hole plates for extracting nucleic acid are arranged in parallel along the X direction on the nucleic acid extraction consumable position and used for placing various consumables, the nucleic acid extraction module 7 moves along the X direction when working during nucleic acid extraction, and various consumables in the deep-hole plates are used for extracting nucleic acid from sample liquid in the deep-hole plate at the nucleic acid extraction placing position 705. The arrangement of the consumable articles is matched with the movement of the nucleic acid extraction module 7 along the X direction, so that the nucleic acid extraction of the sample liquid in the deep hole plate at the nucleic acid extraction placing position 705 can be quickly started, and the working efficiency is improved. Meanwhile, the nucleic acid extraction module 7 and the consumable parts are positioned at the rear side of the sample application module 8, the nucleic acid extraction module 7 for completing the nucleic acid extraction process and the nucleic acid extraction module 7 for completing the sample application process are arranged in the same space in the front-back direction, the occupied space in the X direction is saved, the whole structure is more compact, the size of the whole machine is reduced, the single machine works in a single mode, and the transfer is facilitated.

The semi-automatic nucleic acid detection equipment further comprises a partition plate 5 which is arranged on the rack 1 and is positioned on one side of the pipetting module 4, and a first opening 50 for the deep hole plate to pass through is arranged on the partition plate 5. The grasping and transferring module 6 moves the deep well plate at the pipetting module 4 to the nucleic acid extraction placement position 705 through the first opening 50. The intermediate baffle plate 5 is connected with the rack 1, the whole structural strength of the rack 1 is increased by the intermediate baffle plate 5, and the intermediate baffle plate 5 plays a role in supporting each module to reduce the weight of the rack 1, thereby reducing the weight of the whole machine and facilitating the transfer and the transportation. Meanwhile, the partition plate 5 is arranged to prevent the sample liquid from dripping outside and polluting each other in the sample stopping process and the liquid transferring process.

Preferably, the rack 1, the uncovering module 2, the clamping module 3, the pipetting module 4, the partition plate 5, the clamping and transferring module 6, the nucleic acid extracting module 7 and the sample application module 8 are all arranged in a casing which is arranged on the rack 1. In order to guarantee above-mentioned each module during operation, be in basic confined space, prevent inside entering equipment such as outside dust impurity, simultaneously, also guarantee outside staff's safety.

Preferably, the semi-automatic nucleic acid detecting apparatus further includes an air cleaning module (not shown) disposed on the rack 1 and located on the top of the lid opening module 2. The air purification module is arranged to ensure that the space in the whole working space of the equipment is clean when each module in the machine shell works, so that the accuracy of a detection result is prevented from being influenced, and external impurity dust is prevented from entering the working space.

Preferably, as shown in fig. 2 to 6, the uncovering module 2 comprises an uncovering Y-direction loading assembly 21, an uncovering X-direction moving assembly 24, an uncovering Z-direction moving assembly 25, a grabbing assembly 26 and a buffer assembly 27, wherein the uncovering Y-direction loading assembly 21 is arranged on the working platform, a sample tube placing table 22 is arranged on the uncovering Y-direction loading assembly 21, the uncovering Y-direction loading assembly 21 can drive the sample tube placing table 22 to move along the Y direction, and the sample rack 200 and the sample tubes 300 thereon are placed on the sample tube placing table 22. The uncovering X-direction moving assembly 24 is arranged on the rack, the uncovering Z-direction moving assembly 25 is arranged on the uncovering X-direction moving assembly 24, and the uncovering X-direction moving assembly can drive the Z-direction moving assembly to move along the X direction. The buffer member 27 is connected to the door Z-direction moving member 25 and is also connected to the gripper member 26, and the door Z-direction moving member 25 can drive the gripper member 26 and the buffer member 27 to move in the Z-direction simultaneously. When the grabbing component 26 is pressed against the upper cover of the sample tube 300, the buffer component 27 can drive the grabbing component 26 to move along the Z direction in a self-adaptive manner. The uncap X is to moving subassembly 24 drive and snatch subassembly 26 and move along X to, uncap Z is to moving subassembly 25 drive and snatch subassembly 26 and move along Z to, simultaneously, cooperation sample tube placing table 22 can drive sample frame 200 and sample tube 300 above that and move along Y to, realizes three-way movement. The grasping assembly 26 can grasp the sample tube 300 to move to the clamping module 3 and rotate to open the upper cover of the sample tube 300, and screw the upper cover of the sample tube 300 to the tube body of the sample tube 300 and move from the clamping module 3 back to the sample rack 200. And the grabbing component 26 and the sample tube 300 move simultaneously, so that the adjustment time of the relative positions of the grabbing component 26 and the sample tube 300 is saved, and the working efficiency is improved.

When the height of the sample tube 300 on the sample rack 200 is constant, the coordinate values of the Z-direction movement of the buffer member 27 and the gripper member 26 driven by the lid opening Z-direction movement 25 are the same. When the sample tubes 300 are clamped, the heights of the upper covers of the different sample tubes 300 may be different, and when the grasping assembly 26 grasps the sample tubes 300, the upper covers of the sample tubes 300 are pressed against the grasping assembly 26, and after the grasping assembly 26 is stressed, the grasping assembly 26 moves in the Z direction under the action of the buffer assembly 27 to adapt to the upper covers of the different heights, so that the grasping assembly 26 can grasp the sample tubes 300. In addition, in the embodiment, the heights of the sample racks 200 are adjusted to adapt to the sample tubes 300 of different heights placed thereon, and when the heights of the sample tubes 300 are different, the uncovering Z-direction moving assembly 25 drives the grabbing assembly 26 to move to adjust the positions along the Z direction to adapt to the sample tubes 300 of different heights, so that the sample tubes 300 of different height sizes can be compatible to carry out nucleic acid detection. The range of height dimensions of the sample tube 300 in this embodiment is: sample tubes 300 in the size range of 50mm to 107mm can be placed in the apparatus to be tested.

The sample tube 300 to be detected is placed on the sample frame 200 and transferred to the sample tube placing table 22 together, one of the sample tube placing table 22 and the sample frame 200 is provided with a first positioning piece 221, the other of the sample frame 200 and the sample tube placing table 22 is provided with a first positioning hole, and the first positioning piece 221 is inserted into the first positioning hole to quickly position the sample frame 200, so that the clamping and transferring module 6 can clamp the sample tube 300. In addition, the sample tube placing table 22 is further provided with a first magnetic attraction piece 222, the sample rack 200 is provided with a second magnetic attraction piece, and when the sample rack 200 is placed on the sample tube placing table 22, the first magnetic attraction piece 222 and the second magnetic attraction piece attract each other, so that the sample rack 200 is prevented from moving relative to the sample tube placing table 22 in the moving process.

In this embodiment, the sample rack 200 and the sample tube 300 on the sample rack 200 are manually placed on the sample tube placement platform 22 to start working, after the sample tube 300 on the sample rack 200 is opened and pipetted to the deep hole plate, the clamping and transferring module 6 screws the upper cover of the sample tube 300 back to the tube body of the sample tube 300, and then puts the sample tube on the sample rack 200, and the used sample rack 200 and the sample tube 300 thereon are manually taken away at the same time, and a new sample rack 200 and the sample tube 300 to be tested are placed again.

Preferably, as shown in fig. 2 to 4, the door opening Y-direction loading assembly 21 includes: the first linear motor 211 is disposed on the working platform 100, and an output end of the first linear motor 211 is connected to the first Y-direction linear sliding table 212, the sample tube placing table 22 is disposed on the first Y-direction linear sliding table 212, the first linear motor 211 drives the first Y-direction linear sliding table 212 to drive the sample tube placing table 22 to move along the Y direction, and further drives the sample rack 200 and the sample tube 300 thereon to move along the Y direction. The sample tube 300 and the uncapping module 2 move synchronously, so that the uncapping module 2 and the sample tube 300 move rapidly to a relatively proper position, and the uncapping module 2 can clamp the sample tube 300 conveniently.

Preferably, the uncovering module 2 further comprises a first guide assembly 23 arranged on the working platform 100, the sample tube placing table 22 is connected to the first guide assembly 23, the first guide assembly 23 and the uncovering Y-direction feeding assembly 21 are arranged in parallel, and are spaced by a first preset distance along the X direction, and the uncovering Y-direction feeding assembly 21 can drive the sample tube placing table 22 to move along the first guide assembly 23. When the uncap Y-direction loading unit 21 drives the sample tube placing table 22, the sample rack 200, and the sample tube 300 thereon to move in the Y-direction, the first guide unit 23 assists in guiding the sample tube placing table 22. When the sample tubes 300 with a large number are placed on the sample tube placing table 22, the sample tube placing table 22 is ensured to move stably when moving along the Y direction.

Preferably, the first guide assembly 23 includes a first slide rail 231 disposed along the Y direction on the work platform 100, and a first slider 232 connected to the sample tube placing stage 22, and the first slider 232 slides in cooperation with the first slide rail 231. Slide along first slide rail 231 through first slider 232, and cooperate above-mentioned first linear motor 211 and first Y to sharp slip table 212 to place platform 22 to the sample tube and support and slide, its simple structure need not many occupation space, and compare and set up two sets of above-mentioned Y of uncapping to material loading subassembly 21 side by side, and it is with low costs, be convenient for installation and maintenance.

Preferably, the uncovering X-direction moving assembly 24 comprises an uncovering X-direction sliding table which is arranged on the rack 1, and the uncovering Z-direction moving assembly 25 is arranged on the uncovering X-direction sliding table. In other embodiments, the above-mentioned door opening X-direction moving assembly 24 can further include: the uncovering X is connected with the uncovering X-direction belt pulley component of the output end of the uncovering X-direction movement driving motor, and the uncovering X is connected with the uncovering Z-direction movement component 25.

Preferably, as shown in fig. 2, 5 and 6, the door Z-direction moving assembly 25 includes a door Z-direction driving assembly 251, a door Z-direction driving assembly 252 and a second guide assembly 253, wherein the door Z-direction driving assembly 251 is disposed on the door X-direction moving assembly 24. The cover Z-direction transmission assembly 252 is connected to an output end of the cover Z-direction driving assembly 251. The second guide member 253 is connected to the door Z-drive member 252, and the damping member 27 is connected to the second guide member 253. The uncovering Z-direction driving component 251 drives the uncovering Z-direction transmission component 252 to move along the Z direction and drives the grabbing component 26 and the buffering component 27 to synchronously move along the Z direction, and the second guide component 253 provides guidance for the grabbing component 26 and the buffering component 27 to move along the Z direction so as to ensure that the grabbing component 26 moves stably along the Z direction, accurately grabs the sample tube 300 and cooperates with the clamping module 3 to screw the upper cover of the sample tube 300. The buffer assembly 27 can drive the grabbing assembly 26 to move adaptively along the Z direction.

In this embodiment, the uncapping Z-direction driving component 251 drives the grabbing component 26 and the buffering component 27 to move along the Z direction synchronously, because for the same batch of sample tubes 300 to be opened, the height of the grabbing component 26 moving along the Z direction is the same, and the height is determined according to the minimum height of the sample tubes 300 to be opened, after the grabbing component 26 moves in place along the Z direction, because the heights of the sample tubes 300 placed on the sample tube placing table 22 are different, after the grabbing component 26 contacts with the top of the upper cover, the buffering component 27 can drive the grabbing component 26 to move upwards along the Z direction in a self-adaptive manner, so as to prevent the sample tubes 300 from being excessively pressed downwards by the grabbing component 26. And the sample tubes 300 of different manufacturers have different specifications, when sample tubes 300 of different specifications are placed on the same sample rack 200, the heights of the upper covers of the sample tubes 300 may be different, resulting in different numbers of internal threads of the upper covers. When screwing the upper cap, the grasping assembly 26 grips the upper cap and rotates, and the number of rotations of the grasping assembly 26 is set according to the maximum number of rotations of the internal thread of the upper cap of the batch of sample tubes 300 to be opened. Because the buffering component 27 can drive the grabbing component 26 to adapt to grabbing the upper covers of the sample tubes 300 with different heights, when the grabbing component 26 rotates to screw the upper covers, the buffering component 27 can drive the grabbing component 26 and the screwed upper covers to synchronously move downwards along the Z direction, so that the upper covers clamped by the grabbing component 26 are always ensured to be in opposite positions with the tube bodies, and the upper covers can be smoothly screwed. Therefore, the buffering assembly 27 is provided to screw the upper cover with different turns of the internal thread.

Preferably, the uncovering Z-direction driving assembly 251 comprises an uncovering Z-direction supporting frame 2511 and a second motor 2512 arranged on the uncovering Z-direction supporting frame 2511, wherein the uncovering Z-direction supporting frame 2511 is arranged on the uncovering X-direction moving assembly 24, and an output end of the second motor 2512 is connected to the uncovering Z-direction transmission assembly 252. Preferably, the door opening Z-direction transmission assembly 252 includes a first lead screw 2521 and a first lead screw nut 2522, the first lead screw 2521 is connected to the output end of the motor, and the first lead screw nut 2522 is connected to the second guide assembly 253. In other embodiments, the uncovering Z-direction driving component 251 can also be a component that includes an uncovering cylinder, and an output end of the uncovering cylinder is connected to the uncovering Z-direction transmission component 252. Correspondingly, the uncovering Z-direction transmission assembly 252 comprises a connecting block, the connecting block is connected to the output end of the uncovering cylinder, and the connecting block is connected to the second guide assembly 253 so as to drive the second guide assembly 253 to move along the Z direction.

Preferably, the second guiding assembly 253 comprises a second sliding rail 2531 and a second sliding block 2532 which are matched with each other to slide, and a second guiding connecting member 2533, wherein the second sliding rail 2531 is arranged on the cover opening Z-direction supporting frame 2511, the second sliding block 2532 is connected to the second guiding connecting member 2533, and the second guiding connecting member 2533 is connected to the buffering assembly 27. Adopt second slide rail 2531 and second slider 2532 to mutually support the slip, for snatching subassembly 26 and following the Z direction motion direction, be convenient for accurately grab sample this pipe 300. Meanwhile, the second slide rail 2531 and the second slide block 2532 are adopted, so that the structure is small, the structure is simple, the occupied space of the whole module is small, and the cost is low. The second guiding connection piece 2533 is connected to the second slide block 2532 and is simultaneously connected with the buffering assembly 27 so as to drive the buffering assembly 27 and the grabbing assembly 26 to synchronously move along the Z direction. In other embodiments, the second guiding assembly 253 can also be a guide assembly including: a second sliding groove is formed in the uncovering Z-direction supporting frame 2511, the cross section of the second sliding groove is in a T shape, a second T-shaped sliding block is mounted in the second sliding groove, the second T-shaped sliding block is connected to a second guide connecting piece 2533, and the second guide connecting piece 2533 is connected to the buffer component 27.

Preferably, the damping assembly 27 includes a third guide assembly 271, a third guide connector 272, and a first elastic member 273, wherein the third guide assembly 271 is connected to the second guide connector 2533. The third guiding link 272 is connected to the third guiding element 271, and the third guiding link 272 is connected to the grabbing element 26. One end of the first elastic member 273 abuts against the third guiding connecting member 272, and the other end abuts against the second guiding element 253, and the first elastic member 273 can extend and retract along the Z-direction. When the sample tube 300 is grabbed, the uncovering Z-direction driving assembly 251 drives the grabbing assembly 26 and the buffering assembly 27 to move to a certain position along the Z direction and then stop, the grabbing assembly 26 is adapted to upper covers with different heights, when the grabbing assembly 26 contacts the upper cover of the sample tube 300 downwards along the Z direction, the grabbing assembly 26 is pressed to move along the Z direction, and the first elastic piece 273 is compressed. The grasping member 26 is adaptively moved in the Z direction by the first elastic member 273, so that the upper cover of the sample tube 300 can be more stably grasped. In addition, the third guiding component 271 provides guidance for the adaptive adjustment process of the grabbing component 26 along the Z direction, so as to ensure that the grabbing component 26 stably moves along the Z direction.

The third guiding assembly 271 comprises a third sliding rail 2711 and a third sliding block 2712 which are matched with each other to slide, wherein the third sliding rail 2711 is connected to the second guiding assembly 253, the length direction of the third sliding rail 2711 is arranged along the Z direction, and the third sliding block 2712 is connected to the third guiding connecting piece 272. Utilize third slide rail 2711 and third slider 2712 to cooperate the slip each other, snatch the subassembly 26 and can drive third direction connecting piece 272 and third slider 2712 and slide along third slide rail 2711 to satisfy the fine setting motion accuracy of grabbing subassembly 26 along the Z motion, this simple structure arranges compactly, and is with low costs. Specifically, in this embodiment, the third sliding rail 2711 is connected to the second guiding connector 2533. In other embodiments, the third guiding element 271 can also be, including: a third sliding groove with a length arranged along the Z direction is formed in the third guiding connecting piece 272, the cross section of the third sliding groove is T-shaped, a third T-shaped sliding block is slidably arranged in the third sliding groove, the third T-shaped sliding block is connected to the grabbing component 26, one end of the first elastic piece 273 abuts against the third T-shaped sliding block, and the other end abuts against the third guiding connecting piece 272.

The third guiding connecting member 272 includes a connecting fixing member 2721 and a connecting guiding rod 2722, wherein the connecting fixing member 2721 is connected to the third guiding component 271, the connecting guiding rod 2722 is axially disposed along the Z direction, one end of the connecting guiding rod is connected to the connecting fixing member 2721, the other end of the connecting guiding rod passes through the second guiding component 253, the first elastic member 273 is sleeved on the connecting guiding rod 2722, the first elastic member 273 is located above the connecting fixing member 2721, one end of the first elastic member 273 is pressed against the connecting fixing member 2721, and the other end of the first elastic member is pressed against the second guiding component 253. Specifically, one end of the connecting guide rod 2722 is connected to the connecting fixing piece 2721, and the other end of the connecting guide rod passes through the second guiding connecting piece 2533, one end of the first elastic piece 273 abuts against the connecting fixing piece 2721, and the other end abuts against the second guiding connecting piece 2533. The attachment fixture 2721 is used to attach the grasping member 26, and the grasping member 26 is pressed upward in the Z-direction by the contacted sample tube 300. At this time, the grabbing assembly 26 drives the connecting fixing piece 2721 and the third sliding block 2712 to slide along the third sliding rail 2711. The first elastic member 273 is arranged between the connecting fixing member 2721 and the second guiding connecting member 2533, the first elastic member 273 is located above the connecting fixing member 2721, the first elastic member 273 makes full use of the spatial position between the third sliding rail 2711 and the grasping assembly 26, and meanwhile, the connecting guiding rod 2722 guides the expansion and contraction of the first elastic member 273, so that the first elastic member 273 is prevented from deflecting in the compression process. And the connection mode of the first elastic piece 273, the connection fixing piece 2721 and the connection guide rod 2722 makes the whole assembly compact in structure, and all the components are positioned between the grabbing assembly 26 and the second guide connection assembly 253, so that the layout is reasonable, and the occupied space is small.

Preferably, each group of connecting and fixing pieces 2721 is provided with two connecting guide bars 2722 in parallel along the X direction, each connecting guide bar 2722 is sleeved with a first elastic piece 273, one end of each of the two first elastic pieces 273 abuts against the same connecting and fixing piece 2721, and the other end of each of the two first elastic pieces 273 abuts against the same second guiding and connecting piece 2533. Two sets of first elastic members 273 are arranged to ensure that the stress of the first elastic members 273 is uniform, and the grabbing component 26 moves stably and smoothly in the moving process. Further preferably, the first elastic member 273 is a spring in this embodiment. In other embodiments, a spring guide may be further disposed on the second guiding connection member 2533, the spring guide is provided with a spring guide hole, the upper end of the connection fixing member 2721 is provided with a guide post, one end of the first elastic member 273 abuts against the guide post, the other end of the first elastic member is inserted into the spring guide hole and abuts against the bottom of the spring guide hole, and the guide post can penetrate into or penetrate out of the bottom of the spring guide hole.

Preferably, the grabbing assembly 26 comprises grabbing electric clamping jaws 261 and two sets of first clamping claws 263, wherein the output end of the grabbing electric clamping jaws 261 is provided with a first clamping jaw rotating member 262, the grabbing electric clamping jaws 261 can drive the first clamping jaw rotating member 262 to rotate, and a first clamping claw sliding groove 2621 is arranged on the first clamping jaw rotating member 262. The two sets of first clamping claws 263 are slidably disposed in the first clamping claw sliding grooves 2621, and the transfer electric clamping claws 632 can drive the first clamping claws 263 to slide oppositely or reciprocally along the first clamping claw sliding grooves 2621 so as to clamp or loosen the sample tube 300.

In this embodiment, the grasping electric jaws 261 drive the two sets of first grasping claws 263 to move toward or away from each other, so as to clamp or unclamp the sample tube 300. The maximum distance of the two sets of first jaws 263 moving in the first jaw sliding groove 2621 is the diameter of the largest sample tube 300 to be gripped. By the sliding distance of the two sets of first grippers 263 in the sliding grooves of the first grippers 263, the sample tubes 300 with different diameter size ranges can be gripped.

In this embodiment, the diameter of the upper cover of the sample tube 300 that can be gripped by the first gripper 263 is in the range of 15mm to 24mm. In addition, in the present embodiment, the grasping electric jaw 261 drives the first jaw rotating member 262 and the first grasping claw 263 to rotate synchronously to open the upper cover of the grasped sample tube 300.

Preferably, in this embodiment, the first gripper 263 includes a first gripper body 2631 and two clamping fingers 2632 connected to the first gripper body 2631, wherein the two clamping fingers 2632 are disposed at a predetermined angle along a circumferential direction, and inner side surfaces of the two clamping fingers 2632 are attached to an outer wall of the sample tube 300. Each of the first grips 263 is provided with two clamping fingers 2632, and the sample tube 300 is stably clamped by the four clamping fingers 2632, so that the force applied when the upper cap of the sample tube 300 is screwed is dispersed, and the sample tube 300 can be easily unscrewed or screwed. When the sample tube 300 is not easily unscrewed and needs manual separate processing, the four clamping fingers 2632 are provided, so that the sample tube 300 can be clamped more stably, the upper cover of the sample tube 300 can be unscrewed more easily, the number of sample tubes 300 which are not unscrewed is reduced, and the manual workload is reduced. Preferably, a protrusion is provided on the surface of each gripping finger 2632 that contacts the sample tube 300, and the surface of the gripping finger 2632 is roughened to increase friction gripping the sample tube 300.

Preferably, in this embodiment, a detection component is further disposed on the grasping electric clamping jaw 261, and the detection component can detect the magnitude of the force of the first clamping jaw 263 in real time to determine whether the sample tube 300 is grasped.

In this embodiment, the two sets of uncapping modules 2 are arranged in parallel, and correspondingly, the two sets of clamping modules 3 are arranged to simultaneously clamp the two sample tubes 300 to the clamping modules 3, so as to simultaneously unscrew the upper covers of the two sample tubes 300.

With respect to the specific structure of the clamping module 3, as shown in fig. 1 and 7, and fig. 8a and 8b, the clamping module 3 includes a clamping electric jaw 31 and two second jaws 32, wherein the clamping electric jaw 31 is disposed on the work platform 100. The two second jaws 32 are connected to the output end of the clamping electric jaw 31, and the two second jaws 32 can move towards or towards each other to clamp the tube body of the sample tube 300. In this embodiment, the clamping electric jaw 31 is used to drive the two second jaws 32 to reciprocate so as to clamp the sample tube 300, and the structure is simple, the cost is low, the implementation is easy, and the occupied space is small. In this embodiment, the two sets of clamping modules 3 are used to respectively receive the sample tubes 300 clamped by the two sets of uncapping modules 2. The two sets of gripping modules 3 are arranged side by side along the Y-direction to reduce the occupied space in the X-direction, and are adapted to receive and grip the sample tube 300 gripped by the gripping assembly 26 toward the gripping modules 3. In addition, the distance between the two groups of clamping modules 3 is the same as the distance between the two groups of uncovering modules 2, the two groups of uncovering modules 2 synchronously move along the X direction, the two groups of uncovering modules 2 respectively correspond to the two groups of uncovering modules 2, the relative position movement along the Y direction is reduced, and the working efficiency is improved.

Preferably, the semi-automatic nucleic acid detecting apparatus further comprises a first scan code module 9, and is disposed at one side of the clamping module 3 to scan and record information of the sample tube 300 clamped to the clamping module 3 by the grasping assembly 26.

Preferably, there are two groups of the first scanning modules 9, the two groups of the first scanning modules 9 are respectively located at two sides of the clamping module 3, and one group of the first scanning modules 9 is responsible for scanning and recording information of the sample tubes 300 at one group of the clamping modules 3. In this embodiment, the two groups of first scanning modules 9 and the two groups of clamping modules 3 are arranged along the Y direction, so as to reduce the occupied space and make the whole structure more compact. The layout mode that the two groups of first scanning modules 9 and the two groups of clamping modules 3 are arranged along the Y direction makes full use of the space position between the cover opening module 2 and the liquid transferring module 4, reduces the volume of the whole machine, and meanwhile does not occupy and interfere with the working space of the cover opening module 2 and the liquid transferring module 4.

As shown in fig. 1, 9 and 10, the pipetting module 4 includes a pipetting X-direction moving unit 41, two sets of pipetting Y-direction moving units 42 arranged in parallel, two sets of pipetting Z-direction moving units 43 and a pipetting unit 45, wherein the pipetting X-direction moving unit 41 is arranged on the top of the front side of the rack 1, the pipetting X-direction moving unit 41 is positioned on the front side of the cover opening X-direction moving unit 24, and the mounting positions of the pipetting X-direction moving unit and the cover opening X-direction moving unit are fully utilized in the upper space position on the rack 1, so that the overall structure is compact, and the movement space of the cover opening Z-direction moving unit 25 and the grasping unit 26 is not occupied. Specifically, the above-described pipetting X-direction moving unit 41 is located on one side of the partition plate 5. The two sets of liquid transfer Y-direction moving units 42 arranged in parallel are connected to the liquid transfer X-direction moving unit 41, and the liquid transfer X-direction moving unit 41 can drive the Y-direction moving units to move in the X direction in synchronization. The two sets of transfer Z-direction moving units 43 are connected to the transfer Y-direction moving unit 42, and the transfer Y-direction moving unit 42 can drive the transfer Z-direction moving unit 43 to be independently movable in the Y-direction. Each set of pipetting Z-direction moving assembly 43 is connected with a set of pipetting assembly 45, the pipetting Z-direction moving assembly 43 can drive the pipetting assembly 45 to move along the Z direction, and the pipetting assembly 45 is configured to extract the sample liquid in the opened sample tube 300 at the first preset amount of the clamping module 3. In this embodiment, two sets of liquid-transfering components 45 are utilized to move the sample liquid in two sample tubes 300 to the deep hole board simultaneously, and the work efficiency of liquid-transfering is doubled. And the two sets of pipetting Y-direction moving assemblies 42 can independently move along the Y direction and work independently, when the distance between the two sample tubes 300 clamped by the clamping module 2 is different, the sample liquid in the sample tubes 300 can still be simultaneously extracted, and the liquid can be pipetted into the deep-hole plate simultaneously.

The pipetting Z moves the assembly 43 to drive the pipetting assembly 45 to move to the position right above the opened sample tube 300 along the Z direction synchronously until the pipetting assembly 45 extends into the sample tube 300, in the moving process, the pipetting assembly 45 can move finely, the distance of each movement along the Z direction is smaller, so that the moving speed is prevented from being too fast, the problem of descending due to inertia is solved, the depth of the pipetting assembly 45 inserted into the sample tube 300 is difficult to accurately control and contact and collide with the sample tube 300, the pipetting assembly 45 is damaged, and the problem that the depth of the pipetting assembly 45 inserted into the sample tube 300 is insufficient and the sample liquid is not extracted is avoided. Preferably, the pipetting X-direction moving unit 41 is a linear motor.

Preferably, the liquid-transferring Y-direction moving unit 42 includes a liquid-transferring Y-direction fixing plate 421, a liquid-transferring Y-direction driving unit 422, two sets of first belt pulley units 423 arranged in parallel, and a liquid-transferring Y-direction sliding unit 424, wherein the liquid-transferring Y-direction fixing plate 421 is connected to the liquid-transferring X-direction moving unit 41, and the liquid-transferring Y-direction driving unit 422 is provided on the liquid-transferring Y-direction fixing plate 421. Each set of first belt pulley assemblies 423 is connected to the output end of a set of pipetting Y-direction driving assemblies 422, and the pipetting Z-direction moving assembly 43 is connected to the first belt pulley assemblies 423. And a liquid transfer Y-direction sliding assembly 424 which is arranged on the liquid transfer Y-direction fixing plate 421 and is positioned between the two sets of first belt pulley assemblies 423, wherein the two sets of liquid transfer Z-direction moving assemblies are connected to the liquid transfer Y-direction sliding assembly 424 and can independently slide along the Y direction.

In this embodiment, the two pipetting Z-direction moving assemblies 43 share one pipetting Y-direction sliding assembly 424 to move along the Y-direction, thereby saving the space occupied by the structure and reducing the overall structure volume of the pipetting module 4. And move liquid Y to slip subassembly 424 and set up between two sets of first belt pulley subassemblies 423 to guarantee that two sets of move liquid Z to remove liquid subassembly 43 along Y to the in-process of moving, the guide effect that both received is the same with the atress, and the motion is the same, and steady.

Preferably, the pipetting Y-direction slide unit 424 includes a pipetting Y-direction slider 4241 provided on the pipetting Y-direction fixing plate 421 and two pipetting Y-direction slider 4242 slidably provided on the pipetting Y-direction slider 4241, each pipetting Y-direction drive unit 422 is connected to one pipetting Y-direction slider 4242, and the pipetting Y-direction drive unit 422 drives the first belt pulley unit 423 to rotate and drives the pipetting Z-direction moving unit 43 to move in the Y direction. In this embodiment, the two sets of liquid transfer Z-direction moving assemblies 43 share one liquid transfer Y-direction slider 4241 to move in the Y direction, so that the structure is simple, the occupied space of the overall structure of the liquid transfer Y-direction slider assembly 424 is reduced, and the overall structural volume of the liquid transfer module 4 is reduced. Preferably, two sets of first belt pulley subassemblies 423 are located respectively and move liquid Y to the both sides of slip subassembly 424, and two sets of first belt pulley subassemblies 423 all sets up along the Y to, and three's structural arrangement is compact, reduces occupation space. Preferably, the pipetting Y-direction driving assembly 422 comprises a third motor, and an output end of the third motor is connected to the first belt pulley assembly 423.

The liquid transfer Y-direction slider 4241 is a fourth slide rail, the liquid transfer Y-direction slider 4242 is a fourth slider, and the fourth sliders are connected to one liquid transfer Z-direction moving unit 43. In other embodiments, the liquid transfer Y-direction slider 4241 can also be a slide cylinder or a slide cylinder. Move liquid Y to slider 4242 for moving liquid the connecting piece, move liquid the connecting piece and connect in the output of slide cylinder, or the output of slide cylinder, move liquid Z to remove the subassembly 42 and connect in moving liquid the connecting piece. In another embodiment, a liquid transfer Y-direction slide groove may be formed in the liquid transfer Y-direction slider 4241, the cross section of the liquid transfer Y-direction slide groove may be T-shaped, the liquid transfer Y-direction slide block 4242 may be a fourth T-shaped slide block, and the fourth T-shaped slide block may be provided in the liquid transfer Y-direction slide groove to slide in cooperation therewith.

Preferably, the pipetting Z-direction moving assembly 43 comprises a pipetting Z-direction driving assembly 431 and a lead screw nut assembly 432, wherein the pipetting Z-direction driving assembly 431 is connected to the first belt pulley assembly 423 and is also connected to the pipetting Y-direction sliding assembly 424. The screw nut assembly 432 is connected to the output end of the pipetting Z-direction driving assembly 431, the pipetting Z-direction fine-adjusting assembly 44 is connected to the screw nut assembly 432, and the pipetting Z-direction driving assembly 431 drives the screw nut assembly 432 to move and drives the pipetting Z-direction fine-adjusting assembly 44 to move along the Z direction. In this embodiment, the lead screw nut assembly 432 is driven to move by the pipetting Z-direction driving assembly 431, so as to drive the pipetting assembly 45 to move along the Z direction.

In this embodiment, the pipetting Z-direction driving assembly 431 includes a fifth motor, and an output end of the fifth motor is connected to the lead screw nut assembly 432. The output direct connection of fifth motor is in lead screw nut subassembly 432, and fifth motor drive lead screw nut subassembly 432 and drive move liquid fine setting subassembly 44 and move liquid subassembly 45 and set up along Z to synchronous, this simple structure, with low costs, occupation space is little, the maintenance of being convenient for.

In this embodiment, move liquid subassembly 45 and be the structure of moving liquid on the existing market, select according to actual need.

In this embodiment, the pipetting module 4 further includes a pipetting platform 500 disposed on the working platform 100, a pipetting deep well plate placing position 400, a pipetting placing position 401 and a pipetting waste material placing position 402 are sequentially disposed on the pipetting platform 500 along the Y direction, and the deep well plate is placed on the pipetting deep well plate placing position 400 so that the pipetting assembly 45 can move the sample liquid in the sample tube 300 into the deep well plate. The used pipette is manually removed from the waste bucket placed at the waste placement site 402 by manually replenishing the deep-well plate, pipette, and the like at each of the placement sites. The three placing positions are sequentially arranged along the Y direction, so that the occupied space is reduced, and the size of the whole machine is reduced.

Preferably, the work platform 100 is provided with a pipetting Z-direction moving assembly 46, the pipetting platform 500 is provided on the pipetting Z-direction moving assembly 46, and the pipetting Z-direction moving assembly 46 can drive the pipetting platform 500 to move along the Y direction.

The pipetting Z-direction moving assembly 46 is arranged to drive the working platform 100 to move along the Y direction, so that a worker can conveniently take and place the deep hole plate, the pipette and the waste bucket on the three placing positions. The structure realizes the relative movement with the pipetting assembly 45 along the Y direction, the pipetting X-direction moving assembly 41 makes the pipetting assembly 45 move along the X direction, the pipetting Z-direction moving assembly 43 makes the pipetting assembly 45 realize the Z-direction movement, and the pipetting Z-direction moving assembly 46, the pipetting X-direction moving assembly 41 and the pipetting Z-direction moving assembly 43 are arranged up and down along the Z direction, so the space occupation is reasonable and the structure is compact.

The specific structure of the pipetting Z-direction moving assembly 46 is the same as that of the decapping Y-direction loading assembly 21 described above.

In other embodiments, the pipetting Z-direction moving assembly 46 can also be such that it includes a pipetting Y-direction feeding motor and a pipetting belt pulley assembly, wherein the pipetting Y-direction feeding motor is disposed on the work platform 100. Move liquid belt pulley assembly and connect in the output that moves liquid Y to the material loading motor, and it connects in moving liquid platform 500.

With respect to the specific structure of the gripping and transferring module 6, as shown in fig. 1, fig. 11, fig. 12, fig. 13a, and fig. 13b, the gripping and transferring module 6 includes a transfer Y-direction moving assembly 61, a transfer Z-direction moving assembly 62, and a transfer gripping assembly 63, wherein the transfer Y-direction moving assembly 61 is provided on the door X-direction moving assembly 24, and the door X-direction moving assembly 24 in this embodiment is provided in the X direction through the partition plate 5. The transfer Z-direction moving unit 62 is provided in the transfer Y-direction moving unit 61, and the transfer Y-direction moving unit 61 drives the transfer Z-direction moving unit 62 to move in the Y-direction. The transferring and clamping assembly 63 is arranged on the transferring Z-direction moving assembly 62, and the transferring Z-direction moving assembly 62 can drive the transferring and clamping assembly 63 to clamp the deep hole plate and drive the deep hole plate to rotate.

In this embodiment, the transfer Y-direction moving assembly 61 and the liquid transfer Y-direction moving assembly 42 are disposed on the same cover opening X-direction moving assembly 24, so that the occupied space of the whole machine is saved. In addition, the transfer Z-direction moving assembly 62 can drive the transfer clamping assembly 63 to move along the Z direction, can clamp the deep hole plate, and can drive the clamped deep hole plate to rotate. In this embodiment, the transfer gripper assembly 63 can pass through the first opening 50 of the partition plate 5, grip the deep well plate at the pipetting module 4, and move to the nucleic acid extraction module 7 to extract nucleic acids. After the nucleic acid extraction is complete, the transfer gripper assembly 63 transfers the deep well plate to the spotting module 8 where a second predetermined amount of liquid is drawn into the spotting plate.

The above-mentioned gripping transfer module 6 works in the right space of the partition plate 5, and can move in the X, Y and Z directions to transfer the deep well plate to and from the pipetting module 4, the nucleic acid extracting module 7 and the spotting module 8.

In the specific structure of the shift Y-direction moving assembly 61, it is preferable that the shift Y-direction moving assembly 61 includes a shift Y-direction fixing plate 611, a shift Y-direction driving assembly 612, two sets of second belt pulley assemblies 613 and a shift Y-direction sliding assembly 614, wherein the shift Y-direction fixing plate 611 is disposed on the door opening X-direction moving assembly 24, the shift Y-direction driving assembly 612 is disposed on the shift Y-direction fixing plate 611, each set of second belt pulley assemblies 613 is connected to an output end of the set of shift Y-direction driving assemblies 612, and the shift Z-direction moving assembly 62 is connected to the second belt pulley assemblies 613. The transfer Y-direction sliding assembly 614 is disposed on the transfer Y-direction fixing plate 611 and between the two sets of second belt pulley assemblies 612, and both the transfer Z-direction moving assembly and the spotting module 8 are connected to the transfer Y-direction sliding assembly 614 and can slide independently along the Y-direction.

In this implementation, sample application module 8 and transfer Z move a set of Y to sliding component 614 to removal subassembly 62 jointly, and both move to X along the synchronization, transfer clamping component 63 and when moving the deep hole board that nucleic acid extraction module 7 extracted nucleic acid to sample application department and carrying out the sample application, sample application module 8 moves to sample application department in step, again along Y to with Z to the adjustment position can, sample application module 8 and transfer Z move the subassembly 62 synchronous motion process to saving time, improve work efficiency.

The second pulley assembly 613 and the first pulley assembly 423 have the same structure, and the specific structure of the transfer Z-direction moving assembly 62 is the same as that of the liquid transfer Z-direction moving assembly 43, and thus, detailed description thereof is omitted.

Specifically, the transfer Y-direction sliding unit 614 is disposed on the transfer Y-direction fixing plate 611, the liquid-transferring Y-direction sliding unit 424 includes a transfer Y-direction slider, and two sets of transfer Y-direction sliding blocks 6141 slidably disposed on the transfer Y-direction slider. The transfer Z-direction moving unit 62 is connected to one of the transfer Y-direction sliders 6141, and the transfer Z-direction moving unit 62 is connected to one of the second belt pulley units 613. The print module 8 is connected to another set of transfer Y-direction sliders 6141 and the print module 8 is simultaneously connected to another set of second belt pulley assemblies 613.

Preferably, the transfer clamping assembly 63 includes a transfer Z-direction guiding assembly 631, a transfer electric clamping jaw 632 connected thereto, and a clamping arm 634, wherein the transfer Z-direction guiding assembly 631 is connected to the transfer Z-direction moving assembly 62, and the transfer Z-direction guiding assembly 631 provides a guide for the transfer electric clamping jaw 632 to move along the Z direction. The output end of the transfer electric clamping jaw 632 is provided with a rotary clamping block 633, a clamping sliding chute 6331 is arranged on the rotary clamping block 633, and a clamping sliding block 6332 which slides in a matched manner is arranged in the clamping sliding chute 6331. Attached to each clamp slide 6332 is a set of clamp arms 634, the two sets of clamp arms 634 being movable in the same direction towards each other or towards each other, the clamp arms 634 being configured to clamp both sides of the deep hole plate.

When the transfer Z-direction moving assembly 62 drives the transfer electric clamping jaw 632 to move along the Z direction, the transfer Z-direction guiding assembly 631 guides the transfer electric clamping jaw 632 to ensure the stability of the movement of the transfer electric clamping jaw 632. Meanwhile, the transfer Z-direction guide assembly 631 is positioned between the transfer Z-direction moving assembly 62 and the transfer electric clamping jaw 632, and the transfer clamping assembly 63 and the transfer Z-direction moving assembly 62 are compact in structure and small in occupied space, so that the size of the whole machine is reduced.

Preferably, as shown in fig. 12, 13a and 13b, the clamp arm 634 includes a clamp arm body 6341, a clamp movable block 6342, a connecting rotation member 6343 and a second elastic member 6344, wherein one end of the clamp arm body 6341 is connected to the clamp slider 6332, the clamp movable block 6342 is connected to the front end of the clamp arm body 6341, and a wear pad 6345 is disposed between the clamp arm body 6341 and the clamp movable block 6342. The connecting rotation member 6343 is disposed along the Z-direction in the axial direction, and passes through the clamp arm body 6341, the wear pad 6345, and the clamp movable block 6342 is capable of rotating relative to the clamp arm body 6341 by a first preset angle in the Z-direction. The second elastic member 6344 is disposed between the clamping movable block 6342 and the connecting rotation member 6343, and an axial direction of the second elastic member 6344 is perpendicular to a side wall of the deep hole plate clamped by the clamping arm body 6341.

In this embodiment, when the clamping arm body 6341 clamps the side wall of the deep hole plate, the side wall of the deep hole plate is not a vertical plane due to a dimension error, and the vertical distance between the two side walls of different deep hole plates is different, that is, there is an error in the width dimension of the deep hole plate, therefore, when the clamping arm body 6341 and the clamping movable block 6342 are both in contact with the deep hole plate, the clamping arm body 6341 and the clamping movable block 6342 are rotated by a small angle through the connecting rotating member 6343, the second elastic member 6344 is disposed between the two, and the relative position between the two can be adaptively adjusted by rotation, so as to adapt to contact and fit with the side wall of the deep hole plate, and stably clamp the deep hole plate.

Preferably, a set of second elastic members 6344 is disposed on both sides of the connecting rotation member 6343 in this embodiment. The two sets of second elastic pieces 6344 are arranged to ensure that the two sets of second elastic pieces 6344 can ensure that the clamping movable block 6342 is balanced after the clamping movable block 6342 and the clamping arm body 6341 rotate relatively, and the clamping movable block 6342 can be completely attached to the deep hole plate. Preferably, the second elastic member 6344 is a spring.

Preferably, the gripping and transferring module 6 further includes a transfer Y-direction slider 6142 disposed on the transfer Y-direction fixing plate 611, and two sets of transfer Y-direction sliders 6141 slidably disposed on the transfer Y-direction slider 6142. A second code scanning module 64 on the clamp arm 634, the second code scanning module 64 configured to scan information of the deep hole plate gripped by the transfer clamp assembly 63. In this embodiment, the second code scanning module 64 is provided for scanning the information on the deep well plate gripped by the transfer gripper assembly 63, so as to scan and record the information on the deep well plate gripped from the pipetting module 4, and simultaneously, the information on the deep well plate after nucleic acid extraction by the gripper assembly 7 is scanned, so as to compare and record the information on the deep well plate, thereby preventing errors.

Preferably, as shown in fig. 11 and 14-15, the spotting module 8 includes a spotting Y-direction platform assembly 811 disposed on the working platform 100, the spotting Y-direction platform assembly 811 is provided with a spotting platform 812, the spotting platform 812 is sequentially provided with a spotting deep-hole plate placing position 800, a spotting plate placing position 801, a spotting needle tube placing position 802, and a spotting waste material placing position 803 from the spacer 5 along the X direction, and the spotting Y-direction platform assembly 811 can drive the spotting platform 812 to move back and forth along the Y direction. The spot position described above is a point sample depth well plate position 800.

The specific structure of the spotting Y-direction platform assembly 811 in this embodiment is the same as the specific structure of the decap Y-direction feeding assembly 21, and is not described herein again.

The spotting deep well plate placing position 800 is placed with the deep well plate gripped from the pipetting module 45 and returned thereto from the nucleic acid extraction module 7. The spotting plate placing station 801 is used for placing unused spotting plates, and the spotting tube placing station 802 is used for placing unused spotting tubes. The sample waste placement site 803 is used to place a used sample syringe.

The sample application Y drives the sample application platform 812 to work along the Y direction to the platform assembly 811, and the sample application platform 812 moves along the Y direction synchronously with the transfer clamping assembly 63 and the sample application module 8, so that the deep hole plate and the sample application plate on the sample application platform 812 can be quickly positioned at proper positions, and the transfer clamping assembly 63 and the sample application module 8 can work conveniently, thereby improving the working efficiency.

In addition, the spotting platform 812 is located at the front side of the rack 1, and the spotting platform 812 can move each placing position thereon to the front door of the rack 1, so that the worker can supplement the materials and take away the used materials, that is, an unused spotting plate and a spotting needle tube are placed at each placing position, and the used spotting waste is taken away.

To the concrete structure of the spotting module 8, it further comprises a spotting Z-direction moving component 82 and a spotting component 83, wherein the spotting Z-direction moving component 82 is disposed on the transfer Y-direction moving component 61 and can move along the Y-direction. The printing component 83 is connected to the printing Z-direction moving component 82, and the printing Z-direction moving component 82 can drive the printing component 83 to move along the Z-direction.

In this embodiment, the spotting Z-direction moving module 82 drives the spotting Z-direction moving module 82 to move in the Z-direction, and the sample liquid in the deep well plate from which the nucleic acid has been extracted in the deep well plate is transferred into the spotting plate by sucking a second predetermined amount in accordance with the movement in the Y-direction and the X-direction. The sample application module 8 and the clamping and transferring module 6 synchronously act along the X direction by depending on the same liquid transferring X direction moving assembly 41, so that the working efficiency is improved, the structural arrangement of respective movement of each module is reduced, the structural volume of the whole machine is reduced, and the cost is reduced. The spotting component 83 is a conventional spotting structure on the market, and can be selected according to actual needs. The specific structure of the spotting Z-direction moving assembly 82 in this embodiment is the same as the specific structure of the transfer Z-direction moving assembly 62, and is not described herein again.

As shown in FIG. 1, FIG. 14-FIG. 20, the nucleic acid extraction module 7 is disposed behind the clamping and transferring module 6 and the spotting module 8, which are all located on the same side of the partition plate 5, so as to reasonably utilize the front and rear space positions in the rack 1, thereby achieving compact structural layout and reducing the volume of the whole machine.

To the concrete structure of the above-mentioned nucleic acid extraction module 7, it includes that set up on the spotting platform 812 from the partition 5 along the X to a plurality of deep well plate placing positions that set gradually, the deep well plate placing position is located the rear side of spotting deep well plate placing position 800, wherein, the deep well plate placing position of the farthest position department apart from partition 5 is configured as to place and shifts the deep well plate after the liquid-transfering that clamping assembly 63 got from spotting deep well plate placing position 800 clamp. The plurality of deep-hole plate placing positions comprise a first deep-hole plate placing position 700, a second deep-hole plate placing position 701, a third deep-hole plate placing position 702, a fourth deep-hole plate placing position 703, a fifth deep-hole plate placing position 704 and a nucleic acid extraction placing position 705 which are sequentially arranged along the X direction from the partition plate 5. The first to fifth deep well plate placing locations 700 to 704 are the nucleic acid extraction consumable locations described above, and are configured to place different kinds of consumables or solvents necessary for nucleic acid extraction.

And above-mentioned each deep hole board is placed position and sample application deep hole board and is placed position 800, sample application board and place 801 and sample application needle tube and place 802 and sample application waste material and place position 803 on same sample application platform 812, and above-mentioned each is placed the position homoenergetic and is followed Y to the motion in step to place the material to above-mentioned each is placed on the position. Simultaneously, above-mentioned each place the position homoenergetic and to moving along Y in step, and be two rows along X to arranging, reduce the occupation space in the horizontal plane, rationally distributed, can satisfy the workspace requirement of getting transfer module 6 and sample application module 8 at the transfer clamp of front side, can satisfy the workspace requirement of nucleic acid extraction module 7 at the rear side again.

As shown in fig. 16-20, the nucleic acid extraction module 7 further includes a nucleic acid extraction X-direction driving assembly, a nucleic acid extraction rack 72, a nucleic acid extraction Z-direction driving assembly 73, a nucleic acid extraction installation assembly 74, and a nucleic acid extraction assembly 75, wherein the nucleic acid extraction X-direction driving assembly is disposed on the working platform 100 and is located at the rear side of the nucleic acid extraction deep-well plate placement position. The nucleic acid extraction rack 72 is disposed on a nucleic acid extraction X-direction drive assembly that can drive the nucleic acid extraction rack 72 to move in the X-direction. The nucleic acid extraction Z-direction driving assembly 73 is arranged on the nucleic acid extraction rack 72, the nucleic acid extraction mounting assembly 74 is connected to the nucleic acid extraction Z-direction driving assembly 73, the nucleic acid extraction mounting assembly 74 can synchronously move along the Z direction relative to the nucleic acid extraction rack 72, and the nucleic acid extraction mounting assembly 74 can clamp or loosen the magnetic rod sleeve. The nucleic acid extraction assembly 75 is arranged right above the nucleic acid extraction mounting assembly 74 and is connected to the output end of the nucleic acid extraction mounting assembly 74, and the nucleic acid extraction mounting assembly 74 can drive the nucleic acid extraction assembly 75 to move along the Z direction relative to the nucleic acid extraction rack 72, so that the nucleic acid extraction assembly 75 can be installed on or removed from the magnetic rod sleeve.

In this embodiment, each of the deep well plate placement positions can be moved in the Y direction to a position directly below the nucleic acid extraction module 75, the nucleic acid extraction X-direction driving module can drive the nucleic acid extraction module 75 to move in the X direction, and the nucleic acid extraction Z-direction driving module 73 can drive the nucleic acid extraction module 75 to move in the Z direction, so that the nucleic acid extraction module 75 moves into the deep well plate for nucleic acid extraction. The nucleic acid extraction mounting assembly 74 is located directly below the nucleic acid extraction assembly 75 and is used to hold or release the magnetic sleeve so that the nucleic acid extraction assembly 75 can be inserted into or removed from the magnetic sleeve. The magnetic rod sleeve in this embodiment is placed at the position where one of the deep hole plates is placed.

Preferably, the nucleic acid extracting X-direction driving assembly includes a nucleic acid extracting X-direction motor module disposed on the working platform 100 and located at the rear side of the spotting platform 812, and the nucleic acid extracting rack 72 is connected to the nucleic acid extracting X-direction motor module. The X-direction motor module for extracting the nucleic acid in the embodiment is a conventional electric cylinder, the type of the motor module can be selected according to actual needs, and the motor module is low in cost and convenient to use.

Preferably, the nucleic acid extraction Z-direction driving assembly 73 comprises a nucleic acid extraction motor 730 and a third belt pulley assembly 732, wherein the nucleic acid extraction motor 730 is arranged on the nucleic acid extraction frame 72, the third belt pulley assembly 732 is arranged on the nucleic acid extraction frame 72, an output end of the third belt pulley assembly 732 is connected to the nucleic acid extraction motor 730, the nucleic acid extraction installation assembly 74 is connected to the third belt pulley assembly 732, and the nucleic acid extraction motor 730 drives the third belt pulley to move and drives the nucleic acid extraction installation assembly 74 to move along the Z-direction. The nucleic acid extraction motor 730 and the third belt pulley component 732 are adopted to drive the nucleic acid extraction installation component 74 to move along the Z direction, the structure is simple, the occupied space is small, the size is small, the replacement is convenient when a fault occurs, and the cost is low. In this embodiment, the third belt pulley assembly 732 is disposed on one side of the nucleic acid extracting rack 72, and the nucleic acid extracting motor 730 is disposed at the bottom of the nucleic acid extracting rack 72, so that the spatial position of the bottom of the nucleic acid extracting rack 72 is fully utilized, and the overall structure is more compact.

Preferably, as shown in fig. 16-20, the nucleic acid extraction installation assembly 74 includes a first installation frame 741, a first installation driving assembly 742, a clamping rod assembly 743 and a rotation driving assembly 744, wherein the first installation frame 741 is connected to the nucleic acid extraction Z-direction driving assembly 73 and is slidably connected to the nucleic acid extraction frame 72, and the first installation frame 741 is provided with a second opening 70 for the magnetic rod of the nucleic acid extraction assembly 75 to pass through. The first mounting driving element 742 is disposed on the first mounting frame 741, and an output end of the first mounting driving element 742 is connected to the nucleic acid extracting element 75, and the first mounting driving element 742 can drive the nucleic acid extracting element 75 to move along the Z-direction. The clamping rod assembly 743 passes through the first mounting frame 741 and is located at two sides of the second opening 70. The rotation driving assembly 744 is disposed on the first mounting frame 741, and is connected to the clamping rod assembly 743, and the rotation driving assembly 744 drives the clamping rod assembly 743 to rotate along its axis to clamp or release the magnetic rod sleeve at the second opening 70. In this embodiment, the nucleic acid extraction Z-direction driving assembly 73 drives the first mounting frame 741 and the structures thereon to move downward along the Z direction, and moves to the deep hole plate placing position located right below the first mounting frame 741 and where the unused magnetic rod sleeve is placed, after the nucleic acid extraction mounting assembly 74 continues to move downward to a proper position, the first mounting driving assembly 742 drives the nucleic acid extraction assembly 75 to move downward, at this time, the rotation driving assembly 744 can drive the clamping rod assembly 743 to rotate so as to clamp the unused magnetic rod sleeve, and the magnetic rod of the nucleic acid extraction assembly 75 is inserted into the magnetic rod sleeve. After the magnetic rod of the nucleic acid extraction assembly 75 is inserted into the magnetic rod sleeve, the nucleic acid extraction Z-direction driving assembly 73 drives the nucleic acid extraction assembly 75 and the nucleic acid installation assembly to synchronously lift along the Z direction, and then under the driving of the nucleic acid extraction X-direction driving assembly, the nucleic acid extraction Z-direction driving assembly synchronously moves along the X direction to the deep-hole plate clamped to the nucleic acid extraction placing position 705 by the clamping and transferring module 6 to perform nucleic acid extraction work.

Preferably, as shown in fig. 16, 18 to 20, the rotational driving unit 744 includes a pressing member 7441, a tension spring 7442 and a pressing contact member 7443, wherein the pressing member 7441 is provided on the nucleic acid extracting unit 75 and can move synchronously with the nucleic acid extracting unit 75 in the Z direction. One end of the tension spring 7442 is connected to the first mounting frame 741, and the other end is connected to the clamping rod assembly 743, and the axial direction of the tension spring 7442 forms a second preset angle with the X direction. The contact 7443 is disposed on the clamping rod assembly 743, and when the first mounting driving assembly 742 drives the nucleic acid extracting assembly 75 to move downward along the Z direction, the contact 7443 can slide along the contact 7441 and contact the engaging portion of the contact 7441, and the tension spring 7442 can drive the clamping rod assembly 743 to rotate to release the magnetic rod sleeve. The second predetermined angle is an obtuse angle.

In this embodiment, the contact 7443 slides along the contact 7441 during the downward movement along the Z direction until contacting the engaging portion of the contact 7441, and at this time, the extension spring 7442 is stretched, and the axis of the extension spring 7442 forms a second predetermined angle with the X direction, so that after the contact 7443 moves downward to engage with the engaging portion, the extension spring 7442 is stretched to drive the clamp rod assembly 743 to rotate for opening, and the used magnetic rod sleeve is released and placed at the deep hole plate placing position, and the specific position thereof may be selected from the first deep hole plate placing position 700 to the fifth deep hole plate placing position 704 according to actual requirements. In addition, after the clamping rod assembly 743 is placed in the used magnetic rod sleeve, the magnetic rod assembly 743 is moved to the deep hole plate placing position where the unused magnetic rod sleeve is placed, the first installation driving assembly 742 drives the nucleic acid extracting assembly 75 to descend, at this time, the pressing contact 7443 is disengaged from the clamping part of the pressing part 7441 and starts to slide along the pressing part 7441, the clamping rod assembly 743 rotates to clamp the unused magnetic rod sleeve, and the magnetic rod of the nucleic acid extracting assembly 75 is inserted into the magnetic rod sleeve. Thereafter, the nucleic acid extraction mounting assembly 74 and the nucleic acid extraction assembly 75 are synchronously raised in the Z direction and moved in the X direction to the position of the nucleic acid extraction site 705 on the spotting platform 812 to extract the nucleic acid of the sample liquid therein.

In this embodiment, the tension spring 7442 is connected to the clamping rod assembly 743, the pressing contact member 7443 and the pressing member 7441 are pressed against each other, and the tension spring 7442 drives the clamping rod assembly 743 to rotate, so as to clamp or loosen the deep hole plate. In other embodiments, the rotation driving assembly 744 can also be such that it comprises a rotation motor, wherein the rotation motor is arranged on the first mounting frame 741, and an output end of the rotation motor is connected to the clamping rod assembly 743.

The specific structure of the clamping rod assembly 743 includes a rotating rod 7431 and a stopper 7432, wherein the rotating rod 7431 is inserted into the first mounting frame 741, and can rotate in its own axial direction relative to the first mounting frame 741. One end of the stopper 7432 is disposed on the rotating lever 7431, the rotating lever 7431 can rotate with the rotating member, and the other end of the stopper 7432 can clamp the magnetic rod sleeve. In this embodiment, the rotation of the rotating rod 7431 drives the stopping member 7432 to rotate, and the stopping members 7432 on both sides clamp the magnetic rod sleeve, so the structure is simple, the occupied space of the structure is small, and the volume of the whole machine is greatly reduced. Preferably, two abutments 7432 are provided on each rotating rod 7431 along its axial direction at a third predetermined distance in order to provide a uniform and stable clamping of the sleeve.

Preferably, the nucleic acid extraction module 7 further comprises a drip-proof component 76, which is disposed on the nucleic acid extraction rack 72, wherein the drip-proof component 76 can be located right below the nucleic acid extraction component 75 to receive the liquid dropped on the magnetic rod sleeve, and the drip-proof component 76 can be moved away to enable the nucleic acid extraction component 75 to start to work along the Z-direction lifting motion. In this embodiment, the drip-proof component 76 is provided to ensure that after the nucleic acid extraction component 75 extracts nucleic acid in the deep hole plate, the sample liquid on the magnetic rod sleeve drops on other structures, which causes pollution to other structures and affects normal operation.

The drip-proof assembly 76 includes a drip-proof driving assembly 761 and two sets of opposite dropping plate assemblies 762, wherein the drip-proof driving assembly 761 is disposed on the nucleic acid extracting rack 72 and above the nucleic acid extracting assembly 75. Two sets of dropping plate subassembly 762 are located nucleic acid extraction subassembly 75 both sides respectively, and dropping plate subassembly 762 is connected in the output of antidrip liquid drive assembly 761, and antidrip liquid drive assembly 761 can drive two sets of dropping plate subassembly 762 relative motion to the bar magnet cover under, or the both sides that relative motion is located the bar magnet cover. In this embodiment, the drip prevention driving assembly 761 is provided above the nucleic acid extracting assembly 75 to fully utilize the spatial position above the nucleic acid extracting rack 72 without affecting the normal operation of other structures. Set up dropping plate subassembly 762 in the both sides of nucleic acid extraction subassembly 75, utilize the spatial position of nucleic acid extraction subassembly 75 both sides, can not occupy the workspace of other structures, arrange rationally, overall structure is compact.

Preferably, the drip-proof driving assembly 761 includes a first drip-proof driving motor 7611, a gear 7612 and two sets of racks 7613, wherein the first drip-proof driving motor 7611 is disposed on the nucleic acid extracting rack 72 and above the nucleic acid extracting assembly 75. The gear 7612 is connected to an output end of the first drip-proof driving motor 7611. Two sets of racks 7613 set up respectively in the both sides of gear 7612, and all mesh with gear 7612, and rack 763 sets up along X to, and first drip proof driving motor 7611 drive gear 7612 rotates to drive rack 763 along X to the motion. Each set of drip plate assemblies 762 is connected to one of the racks 763, and the rack 763 moves the drip plate in the X direction. In this embodiment, the first drip-proof driving motor 7611, the gear 7612 and the rack 763 are disposed above the nucleic acid extracting assembly 75, and the drip plate assemblies 762 are disposed on two sides of the nucleic acid extracting assembly 75, so that the structure is compact and simple, the occupied space is small, and the cost is low. In other embodiments, the anti-dripping component 76 may further include a second anti-dripping driving motor and a drawing plate, wherein the second anti-dripping driving motor is disposed on the first mounting frame 741. The drawing plate is arranged on the first mounting frame 741 in a sliding mode, connected to the output end of the second drip-proof driving motor and capable of reciprocating in the Y direction.

For the specific structure of the dropping plate assembly 762, the dropping plate assembly comprises a dropping connecting plate 7621 and a dropping receiving plate 7622, wherein one end of the dropping connecting plate 7621 is connected to the rack 763. The other end of each drip connection plate 7621 is connected to a drip receiving plate 7622, and the two sets of drip receiving plates 7622 can move relative to each other or toward each other. The dropping plate assembly 762 in this embodiment has a simple structure, and the two dropping receiving plates 7622 are located on both sides of the nucleic acid extracting assembly 75 in the X direction, and the space in the X direction is used to reduce the occupation of the space in the Z direction, thereby reducing the height of the whole device and making the whole device compact. Further, this structure facilitates the removal of the drip receiver 7622 for removal, cleaning, and maintenance after the extraction of nucleic acid from the sample liquid in the batch of sample tubes 300 is completed. The nucleic acid extracting unit 75 includes a magnetic rod mounting plate slidably coupled to the nucleic acid extracting rack 72 and a magnetic rod unit mounted on the magnetic rod mounting plate, and the magnetic rod mounting plate is coupled to an output end of the first mounting driving unit 742. The magnetic rod assembly is of an existing structure and can be selected according to actual needs.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A semi-automatic nucleic acid detecting apparatus, comprising:

the device comprises a rack (1), wherein a working platform (100) is arranged on the rack (1), and a cover opening module (2), a liquid transfer module (4), a clamping and transferring module (6), a nucleic acid extraction module (7) and a sampling module (8) which can move back and forth relative to the rack (1) along the X direction, the Y direction and the Z direction are arranged on the rack (1);

the clamping module (3) is arranged on the working platform (100) and is positioned between the uncovering module (2) and the pipetting module (4);

the uncovering module (2) clamps the sample tube (300) on the working platform (100) and moves to the clamping module (3), the clamping module (3) is configured to clamp the tube body of the sample tube (300), and the uncovering module (2) can clamp the upper cover of the sample tube (300) and rotate to open or screw;

the pipetting module (4) extracts a first preset amount of sample liquid from the sample tube (300) with an opened upper cover at the clamping module (3) and moves the sample liquid into a deep hole plate on the working platform (100);

the clamping and transferring module (6) is arranged at one side of the pipetting module (4), the clamping and transferring module (6) is configured to clamp the deep-well plate to a nucleic acid extraction and placement position (705), and the nucleic acid extraction and placement position (705) is arranged on the working platform (100) and is positioned at the nucleic acid extraction module (7);

the nucleic acid extraction module (7) is disposed at the rear side of the gripping transfer module (6) and the spotting module (8), the nucleic acid extraction module (7) being configured to extract nucleic acids of the sample liquid within the deep well plate at the nucleic acid extraction station (705);

the clamping and transferring module (6) moves the deep-hole plate after extracting the nucleic acid to the sample application module (8), and the sample application module (8) extracts a second preset amount of nucleic acid extracting solution in the deep-hole plate to the sample application plate to form a nucleic acid sample to be detected.

2. The semi-automatic nucleic acid detecting apparatus according to claim 1, further comprising:

the partition plate (5) is arranged on the rack (1) and located between the liquid transfer module (4) and the clamping and transferring module (6), a first opening (50) for the deep hole plate to pass through is formed in the partition plate (5), and the clamping and transferring module (6) clamps the deep hole plate at the liquid transfer module (4) and moves to the nucleic acid lifting and placing position (705) through the first opening (50).

3. The semi-automatic nucleic acid detecting apparatus according to claim 1, characterized in that the semi-automatic nucleic acid detecting apparatus further comprises: and the air purification module is arranged on the rack (1) and is positioned at the top of the cover opening module (2).

4. The semi-automatic nucleic acid detecting apparatus according to claim 2, wherein the decap module (2) further comprises:

the cover opening Y-direction feeding assembly (21) is arranged on the working platform (100), a sample tube placing table (22) is arranged on the cover opening Y-direction feeding assembly (21), the cover opening Y-direction feeding assembly (21) can drive the sample tube placing table (22) to move along the Y direction, and a sample rack (200) and a sample tube (300) on the sample rack are placed on the sample tube placing table (22);

the cover opening X-direction moving assembly (24) is arranged on the rack (1);

the uncovering Z-direction moving assembly (25) is arranged on the uncovering X-direction moving assembly (24), and the uncovering X-direction moving assembly (24) can drive the Z-direction moving assembly to move along the X direction;

the grabbing component (26) and the buffering component (27), the buffering component (27) is connected to the cover opening Z-direction moving component (25) and is simultaneously connected to the grabbing component (26), and the cover opening Z-direction moving component (25) can drive the grabbing component (26) and the buffering component (27) to move synchronously along the Z direction;

when the grabbing component (26) is pressed against the upper cover of the sample tube (300), the buffer component (27) can drive the grabbing component (26) to move in a Z-direction self-adaptive mode.

5. The apparatus for semi-automatic nucleic acid detection according to claim 4,

the semi-automatic nucleic acid detection equipment further comprises a first scanning code module (9) which is arranged on one side of the clamping module (3) so as to scan and record information of the sample tube (300) clamped to the clamping module (3) by the grabbing component (26).

6. Semi-automatic nucleic acid detection apparatus according to claim 4, characterized in that the pipetting module (4) comprises:

a liquid-transferring X-direction moving assembly (41) which is arranged on the top of the front side of the rack (1), wherein the liquid-transferring X-direction moving assembly (41) is positioned on the front side of the cover opening X-direction moving assembly (24);

two sets of liquid transfer Y-direction moving assemblies (42) arranged in parallel, both of which are connected to the liquid transfer X-direction moving assembly (41), wherein the liquid transfer X-direction moving assembly (41) can drive the Y-direction moving assembly to move synchronously along the X direction;

two sets of pipetting Z-direction moving assemblies (46) connected to the pipetting Y-direction moving assembly (42), wherein the pipetting Y-direction moving assembly (42) can drive the pipetting Z-direction moving assembly (46) to move independently along the Y direction;

a pipetting assembly (45), wherein each group of the pipetting assembly (46) is connected with one group of the pipetting assembly (45), the pipetting assembly (46) can drive the pipetting assembly (45) to move along the Z direction, and the pipetting assembly (45) is configured to extract a first preset amount of sample liquid in the sample tube (300) which is opened at the clamping module (3).

7. The apparatus for semi-automatic nucleic acid detection according to claim 4, wherein the grasping and transferring module (6) comprises:

a transfer Y-direction moving unit (61) provided in the lid opening X-direction moving unit (24);

a transfer Z-direction moving assembly (62) arranged on the transfer Y-direction moving assembly (61), wherein the transfer Y-direction moving assembly (61) drives the transfer Z-direction moving assembly (62) to move along the Y direction;

and the transfer clamping assembly (63) is arranged on the transfer Z-direction moving assembly (62), and the transfer Z-direction moving assembly (62) can drive the transfer clamping assembly (63) to clamp the deep hole plate and drive the deep hole plate to rotate.

8. The semi-automatic nucleic acid detecting apparatus according to claim 7, wherein the spotting module (8) comprises:

sample application Y to platform subassembly (811), it set up in on work platform (100), sample application Y is provided with sample application platform (812) to platform subassembly (811), sample application platform (812) are gone up along X to from spacer plate (5) department has set gradually sample application deep hole board and has placed position (800), sample application board and has placed position (801), sample application needle tubing and has placed position (802) and sample application waste material and place position (803), sample application Y can drive to platform subassembly (811 sample application platform (812) are along Y to reciprocating motion.

9. The semi-automatic nucleic acid detection apparatus according to claim 8, wherein the nucleic acid extraction module (7) comprises: set up in on sample application platform (812) from the position is placed to a plurality of deep hole boards that set gradually along X to spacer (5), the deep hole board is placed the position and is located the rear side that position (800) was placed to the sample application deep hole board, wherein, the distance the deep hole board of spacer (5) furthest position department is placed the position and is configured to place transfer clamping component (63) follow the sample application deep hole board is placed position (800) and is got the deep hole board after the liquid that moves of clamp.

10. The semi-automatic nucleic acid detection apparatus according to claim 9, wherein the nucleic acid extraction module (7) further comprises:

the nucleic acid extraction X-direction driving assembly is arranged on the working platform (100) and is positioned at the rear side of the deep hole plate placing position;

a nucleic acid extraction rack (72) provided on the nucleic acid extraction X-direction drive assembly, the nucleic acid extraction X-direction drive assembly being capable of driving the nucleic acid extraction rack (72) to move in the X-direction;

a nucleic acid extraction Z-direction drive unit (73) provided on the nucleic acid extraction rack (72);

a nucleic acid extraction mounting assembly (74) connected to the nucleic acid extraction Z-direction driving assembly (73), wherein the nucleic acid extraction mounting assembly (74) can synchronously move along the Z direction relative to the nucleic acid extraction rack (72), and the nucleic acid extraction mounting assembly (74) can clamp or unclamp the magnetic rod sleeve;

and the nucleic acid extraction assembly (75) is arranged right above the nucleic acid extraction mounting assembly (74) and is connected to the output end of the nucleic acid extraction mounting assembly (74), and the nucleic acid extraction mounting assembly (74) can drive the nucleic acid extraction assembly (75) to move along the Z direction relative to the nucleic acid extraction rack (72) so as to enable the nucleic acid extraction assembly (75) to be mounted or detached from the magnetic rod sleeve.

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