CN217997174U

CN217997174U - Color-changing nucleic acid detection system

Info

Publication number: CN217997174U
Application number: CN202221892329.XU
Authority: CN
Inventors: 方明
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-07-21
Filing date: 2022-07-21
Publication date: 2022-12-09
Anticipated expiration: 2032-07-21

Abstract

The utility model discloses a color-changing nucleic acid detection system, relating to the technical field of nucleic acid detection and comprising a shell; the cover body is movably connected with the shell; the heat transfer element is connected inside the shell, more than one accommodating groove and more than one light channel are arranged in the heat transfer element, the light channels are communicated with the bottom surface or the side surface of the accommodating groove, and the accommodating groove is used for accommodating the test tube; more than one luminous unit, is used for through the opening of the holding tank to the test tube emergent visible light; the imaging unit is used for imaging the light rays emitted by the light ray channel; a diffuser or fiber optic connector; a temperature control unit connected to the heat transfer member; and the controller is connected with the light-emitting unit, the imaging unit and the temperature control unit. To the slow technical problem of real-time fluorescence PCR detecting system detection speed, the utility model has the advantages of its detection speed is fast, and with low costs.

Description

Color-changing nucleic acid detection system

Technical Field

The utility model relates to a nucleic acid detects technical field, concretely relates to nucleic acid detecting system discolours.

Background

The nucleic acid detection system is a system for detecting nucleic acid, has the advantage of high precision, and is widely applied to the field of virus detection.

The real-time fluorescence PCR detection system is the most commonly used nucleic acid detection system at present, and detects whether a certain virus exists and the concentration of the virus through fluorescence in a reagent, but the detection speed of the related real-time fluorescence PCR detection system is slow.

SUMMERY OF THE UTILITY MODEL

To the technical problem that the real-time fluorescence PCR detection system detects slowly, the utility model provides a nucleic acid detecting system discolours, its detection speed is fast, and with low costs.

In order to solve the above problem, the utility model provides a technical scheme does:

a color-changing nucleic acid detection system comprising:

a housing;

the cover body is movably connected with the shell;

the heat transfer element is connected to the inside of the shell, more than one accommodating groove and more than one light channel are arranged in the heat transfer element, the light channels are communicated with the bottom surface or the side surface of the accommodating groove, and the accommodating groove is used for accommodating a test tube;

more than one luminous unit, is used for through the opening of the holding tank to the test tube emergent visible light;

the imaging unit is used for imaging the light emitted by the light channel;

a diffuser or fiber optic connector;

a temperature control unit connected to the heat transfer member;

and the controller is connected with the light-emitting unit, the imaging unit and the temperature control unit.

Optionally, the light emitting unit is integrated in the cover.

Optionally, the diffuser or the optical fiber connector is mounted in the light path.

Optionally, the imaging device further comprises a reflective mirror connected to the bottom of the housing, one end of the reflective mirror is arranged opposite to the diffuser, and the other end of the reflective mirror is arranged opposite to the imaging unit.

Optionally, the imaging device further comprises an optical fiber unit, one end of the optical fiber unit is connected with the optical fiber connector, and the other end of the optical fiber unit is opposite to the imaging unit.

Optionally, the optical fiber unit includes:

a fiber optic imaging plate;

a plurality of optical fiber bodies;

one end of the optical fiber body is connected with the optical fiber connector, the other end of the optical fiber body is connected with the optical fiber imaging plate, and one end, far away from the optical fiber body, of the optical fiber imaging plate is opposite to the imaging unit.

Optionally, the imaging unit is more than one photographic imaging unit.

Optionally, the imaging unit is more than one scanning imaging unit.

Optionally, the system further comprises a communication unit, and the communication unit is in communication connection with the controller and the terminal.

Optionally, the display device further comprises a display unit connected with the controller.

Adopt the technical scheme provided by the utility model, compare with prior art, have following beneficial effect:

1) The light diffuser is used for passing the light from the test tube and changing the non-uniform light into uniform light, so that the uniformity of the light passing through the light diffuser is effectively improved, the uniformity of the light entering the imaging unit is further improved, and the imaging precision of the imaging unit is improved, wherein the light diffuser can be particularly milky-white plastic containing a plurality of high-scattering particles;

2) The color-changing nucleic acid detection system is a constant-temperature color-changing PCR rapid nucleic acid detection system, has higher working efficiency than a real-time fluorescent PCR detection system, can obtain a faster detection result than the real-time fluorescent PCR detection system, has short detection time, and can be comparable with the antigen detection speed;

3) The color-changing nucleic acid detection system is a constant-temperature color-changing PCR rapid nucleic acid detection system, has much higher detection precision than that of common antibody detection, is not easy to generate misdiagnosis and missed diagnosis under the similar condition of rapidly outputting a detection result, does not need to perform repeated nucleic acid detection after the antibody detection is finished, and saves labor and cost.

4) One imaging unit replaces a plurality of spectrum color detection channels, so that the cost of the constant temperature color-changing PCR rapid nucleic acid detection system can be greatly reduced, and large-scale popularization and use are facilitated;

5) The cost of one or more cameras or cameras in the imaging unit is low, the system is suitable for various PCR rapid detection systems from small to large (from the least 1 tube to the most 1536 tubes known at present), the cost of the constant temperature color-changing PCR rapid nucleic acid detection system with various scales can be greatly reduced, and the large-scale popularization and use are facilitated;

6) The working distance of the imaging unit is increased by folding the light path through the reflector, increasing the imaging angle of the imaging unit, increasing the number of the imaging units and the like, the height of the equipment is reduced, and the miniaturization of the equipment is facilitated;

7) The light at the bottom of the test tube is guided into one optical fiber imaging plate through the optical fiber unit, and then the optical fiber imaging plate is imaged, so that the height of the device can be greatly reduced, and the device is more convenient to use on a desktop or a multi-layer shelf;

8) The scanning imaging head in the scanner or the copier is used for replacing one or more cameras or cameras to further reduce the height of the equipment, so that the equipment is more convenient to use on a desktop or a multi-layer shelf, the imaging head of the scanner can be matched with an optical fiber imaging plate for use, the imaging of the equipment becomes more flexible, and the equipment becomes flatter;

9) The modular heat transfer element can meet various requirements of various clients to the maximum extent, can provide upgraded service for the clients to meet the possibly increasing requirements, and can greatly reduce the time that the equipment cannot be detected due to the need of returning to the factory for maintenance.

Drawings

FIG. 1 is a schematic structural diagram of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 2 is a second schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 3 is a third schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 4 is a fourth schematic view of the structure of the color-changing nucleic acid detecting system according to the embodiment of the present invention;

FIG. 5 is a fifth schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 6 is a sixth schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 7 is a seventh schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 8 is an eighth schematic structural view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 9 is a ninth schematic view of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

FIG. 10 is a schematic diagram showing a structure of a color-changing nucleic acid detecting system according to an embodiment of the present invention;

in the figure: 1. a housing; 2. a cover body; 3. a heat transfer member; 31. accommodating grooves; 32. a light path; 4. a light emitting unit; 5. a light diffuser; 61. a heating element; 62. a temperature sensor; 7. an imaging unit; 8. a reflector; 9. a handle; 10. an optical fiber unit; 101. a fiber optic imaging plate; 102. an optical fiber body; 11. a communication unit; 12. a display unit; 110. a controller; 111. a rotating shaft; 112. a test tube; 113. a sample; 114. an optical fiber connector.

Detailed Description

For a further understanding of the present invention, reference will be made to the drawings and examples for a detailed description of the invention.

The present application will be described in further detail with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the relevant invention and are not limiting of the invention. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings. The utility model discloses in words such as first, second, be for the description the utility model discloses a technical scheme is convenient and set up, and does not have specific limiting action, is general finger, right the technical scheme of the utility model does not constitute limiting action. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "coupled" are to be construed broadly and encompass, for example, both fixed and removable coupling as well as integral coupling; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. The technical solutions in the same embodiment and between the technical solutions in different embodiments can be arranged and combined to form a new technical solution without contradiction or conflict, which is all within the scope of the present invention.

Example 1

With reference to FIGS. 1-10, this example provides a color-changing nucleic acid detecting system comprising:

a housing 1;

a cover body 2 movably connected with the shell body 1;

the heat transfer element 3 is connected to the inside of the housing 1, more than one accommodating groove 31 and more than one light channel 32 are arranged in the heat transfer element 3, the light channel 32 is communicated with the bottom surface or the side surface of the accommodating groove 31, and the accommodating groove 31 is used for accommodating the test tube 112;

one or more light emitting units 4 for emitting visible light to the test tube 112 through the opening of the accommodating groove 31;

an imaging unit 7 for imaging the light emitted from the light path 32;

diffuser 5 or fiber connector 114;

a temperature control unit connected to the heat transfer member 3;

and a controller 110 connected to the light emitting unit 4, the imaging unit 7, and the temperature control unit.

Specifically, the shell 1 and the cover 2 are movably connected, so that the shell 1 can be opened and closed conveniently, and the shell and the cover are connected through a rotating shaft 111 for relative rotation; the heat transfer element 3 is supported by the housing 1 and is used for carrying the accommodating groove 31, the accommodating groove 31 is used for accommodating test tubes 112 with samples 113, wherein the number of the heat transfer elements 3 is one or more, when the number of the heat transfer elements 3 is one, the heat transfer element 3 can comprise a plurality of accommodating grooves 31, when the number of the heat transfer elements 3 is more, the heat transfer element 3 can comprise one or more accommodating grooves 31 so as to accommodate a plurality of test tubes 112, the specific number of the heat transfer elements 3 can be 1, 8 or 12, and the like, and the heat transfer element is suitable for rapid detection of large-flux thermochromic PCR, wherein the material of the heat transfer element 3 can be specifically a metal material with good heat conductivity such as aluminum, copper, silver, and the like; the light emitting unit 4 may be integrated with the cover body 2 and configured to emit light, and since the light emitting unit 4 is located at one side of the accommodating groove 31, the light emitted by the light emitting unit 4 conveniently enters the test tube 112 located in the accommodating groove 31, where the light emitting unit 4 may specifically be an LED light emitting unit or the like; the diffuser 5 is used for passing the light from the test tube 112 and changing the non-uniform light into uniform light, so that the uniformity of the light passing through the diffuser 5 is effectively increased, the uniformity of the light entering the imaging unit 7 is further increased, and the imaging precision of the imaging unit 7 is improved, wherein the diffuser 5 can be a milky material containing a plurality of high-scattering particles; the temperature control unit maintains the heat transfer member 3 at a constant temperature by heat conduction of the heat transfer member 3, thereby maintaining the test tube 112 at a constant temperature; the imaging unit 7 is located inside the housing 1, and can directly image all the test tubes 112, so as to effectively improve the detection speed, wherein the imaging unit 7 may be specifically a photographic imaging unit or a scanning imaging unit; the controller 110 is used for receiving a signal from the temperature control unit and controlling the on/off of the temperature control unit, so that the temperature control unit can maintain a constant temperature, and the controller 110 is also used for receiving a signal from the imaging unit 7; in order to improve the measurement accuracy, an image processing algorithm may be used to perform a uniform correction on the light spots at the bottom of the test tube 112, assuming that the present color-changing nucleic acid detecting system can perform detection on 64 test tubes 112 at a time, first generating a correction coefficient, in step 1, illuminating each test tube 112 with light of the same brightness to generate a total of 64 light spots, in step 2, obtaining an image by the imaging unit 7, and calculating a measured value of the average brightness of each light spot, in step 3, calculating a maximum value of the average brightness among all the light spots, in step 4, calculating a ratio of the measured value and the maximum value to obtain a correction coefficient for each light spot, in which the process of generating the correction coefficient may be repeated several times so as to obtain several correction coefficients for each light spot, in order to obtain an accurate correction coefficient for each light spot by averaging the obtained several correction coefficients for each light spot, and after obtaining an accurate correction coefficient for each light spot, using the correction coefficients to perform light spot intensity correction, the following steps: after the light spot image is collected each time, the light intensity of each light spot is corrected by using a correction coefficient, namely the obtained light intensity of each light spot is divided by the correction coefficient, so that the light spots at the bottom of each test tube 112 after correction have almost the same brightness, then the accurate color of each light spot is obtained through the red, green and blue components of each light spot, and further whether the color of the reagent in each test tube 112 has obvious change is confirmed, and finally whether the nucleic acid segment of the virus to be detected exists in each test tube 112 is obtained.

It should be noted that the color-changing nucleic acid detecting system has the following measurement modes, (1) single final measurement mode: when the single final measurement mode is used, the present color-changing nucleic acid detecting system collects the brightness of the color of the light emitted from the test tube 112 having the sample 113 for the first time before the temperature is raised, and the nucleic acid fragment of the virus in the sample 113 in the test tube 112 is not raised yet and is multiplied, so that the color of the sample 113 is the original color without the virus, and after the temperature is raised to a target temperature, such as 65 ℃, the present color-changing nucleic acid detecting system starts to time, and after the final set detection time is reached, such as 30 minutes, the brightness of the color of the light emitted from the sample 113 in the test tube 112 for the last time is collected, if the difference between the numerical values of the brightness of the two times is less than a set threshold value, it can be determined that the color of the light emitted from the sample 113 in the test tube 112 has not changed greatly, i.e. the original color without the virus, such as purple, and the color of the light emitted from the sample 113 in the test tube 112 has a virus, such as yellow; (2) multiple final measurement mode: when the color-changing nucleic acid detecting system uses the multiple-time final measurement mode, the brightness of the color of the light emitted from the sample 113 in the cuvette 112 is continuously collected multiple times before the temperature rise is started, and at this time, the nucleic acid fragment of the virus in the sample 113 in the cuvette 112 is not heated yet and is multiplied, so that the color of the sample 113 is the original color without the virus, and after the temperature rise to the target temperature, for example, 65 ℃, the color-changing nucleic acid detecting system starts timing, and after the final set detection time is reached, for example, 30 minutes, the brightness of the color of the light emitted from the sample 113 in the multiple-time continuous collection is measured multiple times, compared with the single-time final measurement mode, the multiple-time final measurement mode is more reliable, because the analysis of the color difference in the single-time final measurement mode only depends on the results of the two times of brightness measurement, if any condition of the two luminance brightness occurs in the acquisition process, deviation and errors of the final detection result can be caused, so that the error-resistant redundancy of the single final measurement mode is weak, in the multiple final measurement mode, if few luminance brightness measurements occur and most of the luminance brightness measurements do not occur, the computing chip can effectively remove or reduce the adverse effect of the detection data with problems on final judgment in a mode of multiple averaging, or a mode of performing median filtering on the data with multiple luminance brightness, or a more complex and effective statistical processing algorithm, such as RANSAC algorithm, on the data with multiple luminance brightness, and therefore the error-resistant redundancy of the multiple final measurement mode is more or less than the error-resistant redundancy of the single final measurement mode; (3) single measurement mode for multiple time points: when the color-changing nucleic acid detecting system is used in a multiple-time single-measurement mode, the brightness data of the color of the sample 113 in the test tube 112 is firstly collected before the temperature rise is started, at this time, after the temperature rise of the nucleic acid fragment of the virus in the sample 113 in the test tube 112 is not yet carried out, thereby multiplying, so that the color of the sample 113 is the original color without the virus, after the temperature is raised to a target temperature, such as 65 ℃, the color-changing nucleic acid detection system starts timing, and then every certain time, for example, every 30 seconds or 1-2 minutes, the lightness data of the color of the sample 113 in the cuvette 112 is collected, until after the final set detection time is reached, for example, 30 minutes, and the lightness data of the color of the sample 113 in the final cuvette 112 is collected, at which point the computing chip can compare the lightness data of the first, and many in the middle, and finally the lightness data of the color of the sample 113 in the test tube 112, to determine whether the color of the light spot at the bottom of each test tube 112 is from the original virus-free color, e.g., purple red, to a viral color, e.g., yellow, the greatest benefit of the single-measurement mode at multiple time points is the ability to measure the viral load in each cuvette 112, if a color change in the sample 113 in the test tube 112 is detected within a short time after the completion of the warming, it can be determined that the viral load in the test tube 112 is high, and, similarly, if a change in the color of the sample 113 in the test tube 112 is detected only after the completion of the temperature increase for a longer period of time, it can be determined that the viral load in the test tube 112 is low, if a change in color of the sample 113 in the test tube 112 has not been detected for a longer period of time after the temperature rise is complete, this indicates that the viral load in the test tube 112 is very low or none; (4) multiple measurement mode for multiple time points: the mode (2) and the mode (3) are combined, namely, each time the luminance data of the color in the test tube 112 is acquired, the luminance data is acquired not once but for many times, so that the adverse effect of individual image which possibly generates errors occasionally on the whole interpretation process can be reduced or effectively eliminated, and the error-resistant redundancy of the equipment is greatly improved.

Further, a light emitting unit 4 is integrated in the cover 2.

Further, the diffuser 5 or the optical fiber connector 114 is installed in the optical fiber passage 32.

Further, the imaging unit 7 is one or more photographic imaging units.

Further, the imaging unit 7 is one or more scanning imaging units.

Further, the heat transfer member 3 is provided with a light passage 32 communicating with the receiving groove 31, and the diffuser 5 is located in the light passage 32.

In particular, the light passage 32 is carried by the heat transfer element 3 and is intended to house the diffuser 5, thus ensuring the stability of the diffuser 5.

Further, the temperature control unit includes a heating element 61 and a temperature sensor 62 both connected to the controller 110, and the heating element 61 is connected to the heat transfer member 3.

Specifically, the heating element 61 is used for receiving the signal of the controller 110 and heating the heat transfer element 3, so as to maintain the temperature of the heat transfer element 3 and thus the temperature of the test tube 112, wherein the heating element 61 may specifically include a resistance heating sheet, a PI film flexible heating sheet, a polyimide film heating film, a heating sheet, or a Peltier temperature control module; the temperature sensor 62 is used for detecting the temperature of the heat transfer member 3 and transmitting a temperature signal to the controller 110, wherein the temperature sensor 62 may be an electronic temperature sensor or an infrared temperature sensor.

Further, a mirror 8 is attached to the bottom of the housing 1, one end of the mirror 8 is disposed opposite to the diffuser 5, and the other end of the mirror 8 is disposed opposite to the imaging unit 7.

Specifically, the reflector 8 is used for reflecting the light from the diffuser 5 to the imaging unit 7, and under the condition of ensuring that the path of the light is unchanged, the distance between the diffuser 5 and the imaging unit 7 can be reduced, so that the height of the shell 1 is reduced, and in addition, two methods for reducing the height of the shell 1 are provided, namely a method I: by increasing the imaging angle of the imaging unit 7, the method two: by increasing the number of imaging units 7.

Further, a handle 9 connected to the cover 2 is included.

Specifically, handle 9 is carried by lid 2, and the user of being convenient for opens and close casing 1.

Further, the optical fiber unit 10 is further included, one end of the optical fiber unit 10 is connected to the optical fiber connector 114, and the other end of the optical fiber unit 10 is disposed opposite to the imaging unit 7.

Specifically, the optical fiber unit 10 is used for transmitting the light from the optical fiber connector 114 to the imaging unit 7, and the distance between the optical fiber connector 114 and the imaging unit 7 can be effectively reduced due to the flexibility of the optical fiber unit 10, so that the height of the housing 1 is reduced, and the color-changing nucleic acid detecting system is flattened.

Further, the optical fiber unit 10 includes:

a fiber optic imaging plate 101;

a plurality of fiber bodies 102;

one end of the optical fiber body 102 is connected with the optical fiber connector 114, the other end of the optical fiber body 102 is connected with the optical fiber imaging plate 101, and one end of the optical fiber imaging plate 101 far away from the optical fiber body 102 is opposite to the imaging unit 7.

Specifically, the optical fiber body 102 is used for receiving light from the optical fiber connector 114 and converging the light onto the optical fiber imaging plate 101; the fiber imaging plate 101 serves to transmit light from the fiber body 102 to the imaging unit 7.

Further, the imaging unit 7 is a camera imager or a scanner imager.

Specifically, the camera imager is a static imager, specifically, a CCD camera imager, a CMSO camera imager and the like; the scanning imager is a mobile imager, and can be specifically a scanner and the like.

Further, a communication unit 11 is included, and the communication unit 11 is in communication connection with the controller 110 and the terminal.

Specifically, the communication unit 11 is configured to implement signal transmission between the controller 110 and the terminal, where the communication unit 11 and the terminal may be connected in a wired manner such as a USB, an ethernet, a serial port or a parallel port, and may also be connected in a wireless manner such as a WIFI or a bluetooth.

Further, a display unit 12 connected to the controller 110 is also included.

Specifically, the display unit 12 is used for receiving a signal from the controller 110 and for displaying.

The present invention and its embodiments have been described above schematically, and the description is not limited thereto, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching of the present invention, without departing from the inventive spirit of the present invention, the person skilled in the art should also design the similar structural modes and embodiments without creativity to the technical solution, and all shall fall within the protection scope of the present invention.

Claims

1. A color-changing nucleic acid detection system, comprising:

a housing;

the cover body is movably connected with the shell;

the imaging unit is used for imaging the light emitted by the light channel;

a diffuser or fiber optic connector;

a temperature control unit connected to the heat transfer member;

2. A color-changing nucleic acid detecting system according to claim 1, wherein said light emitting unit is integrated in said cover.

3. The system of claim 1, wherein the light diffuser or the fiber connector is installed in the light channel.

4. The color-changing nucleic acid detecting system according to claim 1, further comprising a reflecting mirror attached to a bottom of the housing, wherein one end of the reflecting mirror is disposed opposite to the diffuser, and the other end of the reflecting mirror is disposed opposite to the imaging unit.

5. The system of claim 1, further comprising an optical fiber unit, wherein one end of the optical fiber unit is connected to the optical fiber connector, and the other end of the optical fiber unit is disposed opposite to the imaging unit.

6. A color-changing nucleic acid detecting system according to claim 5, wherein the optical fiber unit includes:

a fiber optic imaging plate;

a plurality of optical fiber bodies;

7. The system of claim 1, wherein the imaging unit is one or more photographic imaging units.

8. The system of claim 1, wherein the imaging unit is one or more scanning imaging units.

9. The system of claim 1, further comprising a communication unit, wherein the communication unit is communicatively coupled to the controller and the terminal.

10. The system of claim 1, further comprising a display unit connected to the controller.