CN217973205U - PCR amplification detection device - Google Patents

Abstract

The utility model provides a PCR amplifications detection device, include: a frame; the incubation component is fixed on the frame and comprises a heating seat, a cover plate and a heating element; the heating seat is convexly provided with a plurality of clamping grooves; the cover plate cover is arranged above the heating seat; the heating element is arranged on at least one surface of the cover plate and used for heating the cover plate; the gland assembly comprises a lifting mechanism, a first pressing block, a second pressing block, a plurality of first guide pillars and a plurality of springs; a reading assembly; a translation mechanism to translate the gland assembly. The gland assembly is configured to automatically compress the lid plate of the incubation assembly such that the lid plate is capped over the heating seat to form a dark incubation amplification environment. The cover plate is provided with a heating element, so that the top cover of the PCR tube can keep higher temperature in the incubation and amplification process, liquid in the PCR tube is prevented from evaporating and condensing on the inner surface of the top cover of the PCR tube, and the detection accuracy is improved. The gland assembly plays a role in mild compression through the cooperation of the first pressing block and the second pressing block and the pressing effect of the spring.

Description

PCR amplification detection device

Technical Field

The utility model relates to a nucleic acid amplification check out test set technical field especially relates to a PCR amplifys detection device.

Background

The real-time fluorescence quantitative PCR is a method of adding a fluorescent group into a PCR reaction system, utilizing fluorescence signal accumulation to monitor the whole PCR process in real time, and finally carrying out quantitative analysis on an unknown template through a standard curve. The traditional fluorescence detector has the defects of large volume and complex structure.

SUMMERY OF THE UTILITY MODEL

In order to achieve the above purpose, the utility model is realized by the following technical scheme.

The utility model provides a PCR amplifications detection device, include:

a frame to form a support structure;

the incubation component is fixed on the frame and comprises a heating seat, a cover plate and a heating element; the heating seat is convexly provided with a plurality of clamping grooves for accommodating the PCR tube and heating the PCR tube; the cover plate cover is arranged above the heating seat; the heating element is arranged on at least one surface of the cover plate and used for heating the cover plate;

the gland assembly is arranged above the incubation assembly and comprises a lifting mechanism, a first pressing block, a second pressing block, a plurality of first guide pillars and a plurality of springs; the driving end of the lifting mechanism is connected to the first pressing block and used for driving the first pressing block to move up and down; the top end of the first guide pillar is slidably connected to the first pressing block, and the bottom end of the first guide pillar is fixed to the second pressing block; the spring is sleeved on the first guide pillar and used for buffering the pressing force of the first pressing block;

the reading assembly is used for reading the fluorescence information of the object to be detected in the PCR tube;

a translation mechanism fixed to the rack for translating the capping assembly to allow the heating block to load or reposition the PCR tube;

the first pressing block presses the second pressing block through the spring under the driving of the lifting mechanism, so that the second pressing block presses the cover plate, and the cover plate is covered above the heating seat and abuts against the top cover of the PCR tube.

Preferably, the device further comprises a heat dissipation assembly arranged below the incubation assembly.

Preferably, the heat dissipation assembly comprises a heat sink structure, a fan; the heat sink structure is disposed proximate to the incubation assembly.

Preferably, a heat insulation piece is arranged below the second pressing block.

Preferably, the lifting mechanism comprises a rotating motor, a screw rod, a fisheye ball head, a connecting pin and a fixing block; the top end of the screw rod is connected to an output shaft of the rotating motor, and the bottom end of the screw rod is connected to the fisheye ball head; the connecting pin is sleeved on the fisheye ball head, and two ends of the connecting pin are fixed on the fixing block.

Preferably, the gland assembly comprises a transfer block and a plurality of second guide pillars; the transfer block is connected to the translation mechanism; the lifting mechanism and the second guide pillar are respectively fixed on the transfer block; the first pressing block is connected to the second guide pillar in a sliding mode.

Preferably, the translation mechanism comprises:

two guide rails fixed to the frame;

and the two sliding blocks are fixed on the switching block and are in sliding connection with the matched guide rails.

Preferably, the reading assembly comprises an optical fiber mounting plate, at least one light source, at least one detector, a plurality of incident excitation optical fibers, a plurality of detection collection optical fibers and a light source fixing plate; the optical fiber mounting plate is positioned between the incubation component and the light source fixing plate and is provided with a plurality of first through holes and a plurality of second through holes; the light source and the detector are fixed on the light source fixing plate;

the clamping groove is respectively connected with the first through hole and the second through hole through the incident excitation optical fiber and the detection collection optical fiber.

Preferably, the plurality of first through holes are arranged in a circumferential array to form an outer ring hole structure; the second through holes are arranged in a circumferential array mode to form an inner ring hole structure; the inner ring hole structure is positioned in the outer ring hole structure;

the back of the optical fiber mounting plate is provided with an annular clamping groove which is arranged around the outer side of the inner ring hole structure; the front surface of the light source fixing plate is provided with an annular bulge, and the light source and the detector are respectively positioned on the outer side and the inner side of the annular bulge; the annular bulge is clamped in the annular clamping groove.

Preferably, the reading assembly further comprises a rotary driving member, a reading circuit board; the output shaft of the rotary driving piece is coaxially connected to the light source fixing plate; the reading circuit board is fixed on the back of the light source fixing plate.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the utility model provides a PCR amplifys detection device sets up the gland subassembly to the apron of automatic compressing tightly incubation subassembly makes the apron cover in heating seat top, amplifies the environment with the incubation that forms the dark. The cover plate is provided with the heating element, so that the top cover of the PCR tube is kept at a higher temperature in the incubation and amplification process, liquid in the PCR tube is prevented from evaporating and condensing on the inner surface of the top cover of the PCR tube, and the detection accuracy is improved. When pressing on PCR pipe top cap through the apron, press PCR pipe bottom at the draw-in groove diapire to accelerate the speed of being heated of PCR pipe. The gland assembly plays a role in mild compression through the cooperation of the first pressing block and the second pressing block and the pressing effect of the spring.

The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the specification, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings. The detailed description of the present invention is given by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without undue limitation to the invention. In the drawings:

fig. 1 is a first schematic view of a three-dimensional structure of a device body according to the present invention;

fig. 2 is a structural sectional view of the device body of the present invention;

FIG. 3 is a partially enlarged view of the cross-sectional structure of the device body of the present invention;

fig. 4 is a schematic perspective view of the heating base of the present invention;

fig. 5 is a schematic perspective view of the cover plate of the present invention;

fig. 6 is a schematic perspective view of the adapter of the present invention;

fig. 7 is an exploded view of the reading assembly of the present invention;

fig. 8 is a schematic perspective view of the optical fiber mounting plate of the present invention;

fig. 9 is a schematic view of the optical fiber connection structure of the heating base and the reading assembly of the present invention;

fig. 10 is a schematic perspective view of the device body according to the present invention.

In the figure: 100. a device body;

10. a frame;

20. an incubation assembly; 21. a heating base; 211. a card slot; 2111. a third through hole; 2112. a fourth via hole; 22. a cover plate; 221. a sidewall structure; 222. a groove; 23. a heating member; 24. a transfer seat; 241. a plate body; 2411. a hole of abdication; 242. a first side wall; 242. a second side wall;

30. a gland assembly; 31. a lifting mechanism; 311. a rotating electric machine; 312. a screw rod; 313. a fisheye ball head; 314. a fixed block; 32. a first pressing block; 33. a second pressing block; 34. a first guide post; 35. a thermal insulation member; 36. an adapter; 37. a second guide post;

40. a reading assembly; 41. an optical fiber mounting plate; 411. a first through hole; 412. a second through hole; 42. a light source; 43. a detector; 44. an incident excitation optical fiber; 45. detecting a collection optical fiber; 46. a light source fixing plate; 461. an annular projection; 47. a rotary drive member; 48. a reading circuit board;

50. a translation assembly; 51. a guide rail; 52. a slider; 60. a heat dissipating component; 61. a heat sink structure; 62. a fan.

Detailed Description

The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a more detailed description of the present invention, which will enable those skilled in the art to make and use the present invention. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and generally are not intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.

Example 1

The utility model provides a PCR amplifications detection device, as shown in fig. 1 to fig. 10, including device body 100, device body 100 includes:

a frame 10 to form a support structure;

an incubation assembly 20, fixed to the frame 10, comprising a heating seat 21, a cover plate 22, a heating element 23; the heating base 21 is convexly provided with a plurality of clamping grooves 211 for accommodating the PCR tubes and heating the PCR tubes; the cover plate 22 is covered above the heating seat 21; the heating member 23 is mounted on at least one surface of the cover plate 22 to heat the cover plate 22;

the gland assembly 30 is arranged above the incubation assembly 20 and comprises a lifting mechanism 31, a first pressing block 32, a second pressing block 33, a plurality of first guide pillars 34 and a plurality of springs; the driving end of the lifting mechanism 31 is connected to the first pressing block 32 and is used for driving the first pressing block 32 to move up and down; the top end of the first guide pillar 34 is slidably connected to the first pressing block 32, and the bottom end thereof is fixed to the second pressing block 33; the spring is sleeved on the first guide post 34 and used for buffering the pressing force of the first pressing block 32;

the reading component 40 is used for reading the fluorescence information of the object to be detected in the PCR tube;

a translation mechanism 50 fixed to the rack 10 for translating the capping assembly 30 to allow the heating block 21 to load or reposition the PCR tube;

under the driving of the lifting mechanism 31, the first pressing block 32 presses the second pressing block 33 through the spring, so that the second pressing block 33 presses the cover plate 22, and further the cover plate 22 covers the heating base 21 and abuts against the top cover of the PCR tube.

In this embodiment, the device body 100 is provided with an incubation assembly 20 to provide a PCR amplification incubation environment. The cover plate 22 is placed over the heating seat 21, and the capping assembly 30 is disposed to automatically compress the cover plate 22 of the incubation assembly 20 such that the cover plate 22 is covered over the heating seat 21 to form a dark incubation amplification environment. In addition, the cover plate 22 is provided with a heating element 23, the cover plate 22 is abutted to the top cover of the PCR tube, and the cover plate 22 transfers heat to the top cover of the PCR tube, so that the top cover of the PCR tube keeps higher temperature in the incubation and amplification process, and liquid in the PCR tube is prevented from evaporating and condensing on the inner surface of the top cover of the PCR tube, and the accuracy of a detection result is influenced. Because, the heating effect of draw-in groove 211 diapire is better, when pressing on PCR pipe top cap through apron 22, presses PCR pipe bottom at draw-in groove 211 diapire to accelerate the speed of being heated of PCR pipe. By translating the gland assembly 30 with the translation mechanism 50, the cover plate 22 can be removed, and the bayonet slots 211 opened to remove the PCR tubes. The device body 100 is simple in structure, the loading and taking-out operation of the PCR tube is convenient and efficient, the incubation of the PCR tube is efficient, and the detection is accurate. In addition, the gland assembly 30 reduces the rigid force when the second pressing block 33 presses on the cover plate 22 through the cooperation of the first pressing block 32 and the second pressing block 33 and the spring pressing effect, and plays a role in mild compression.

In one embodiment, the cover plate 22 is provided with a sidewall structure 221 to cover the heating seat 21 to form a dark incubation and amplification environment together with the heating seat 21.

In one embodiment, the cover 22 has a recess 222 formed in an upper surface thereof for receiving the heating element 23.

In one embodiment, the heating member 23 is a heating film, thin, and fast to heat.

In an embodiment, the incubation assembly further comprises an adaptor 24, said adaptor 24 comprising a plate body 241; the plate body 241 extends circumferentially upwards to form a first side wall 242 and extends downwards to form a second side wall 243; the plate body 241 is provided with a plurality of yielding holes 2411; the adapter 24 covers the heating base 21, the second side wall 243 surrounds the heating base 21, a slot 211 penetrates out of the yielding hole 2411 from bottom to top, and the cover plate 22 covers the plate body 241 and surrounds the yielding holes 2411. The cover plate 22 is carried by the adapter 24, and the thickness of the adapter 24 is increased to form a heat insulation structure, so that the heat of the heating base 21 is prevented from being transferred to the outside through the cover plate 22. The first sidewall 242 of the adapter 24 partially or completely surrounds the cover plate 22 to reduce the rate of heat transfer between the cover plate 22 and the heating base 21 out of the cover plate 22 and increase the heating effect of the incubation assembly 20.

In one embodiment, the device body 100 further comprises a heat sink assembly 60 disposed below the incubation assembly 20. Specifically, the heat of the heating seat 21 of the incubation assembly 20 is timely reduced by the heat dissipation assembly 60, so that the heating temperature of the heating seat 21 can be adjusted to meet the temperature-up-down alternating cycle requirement required by PCR amplification.

Further, the heat dissipation assembly 60 includes a heat sink structure 61, a fan 62; the heat sink structure 61 is disposed proximate to the incubation assembly 20. Specifically, the fan 62 is configured to generate a heat dissipating airflow, and the heat dissipating fin structure 61 absorbs the cold energy of the heat dissipating airflow and transfers the cold energy to the heating base 21 to cool the heating base 21.

In an embodiment, a heat insulation member 35 is disposed below the second pressing block 33. Specifically, since the cover plate 22 is heated, the temperature is high, so as to avoid the temperature of the cover plate 22 being transferred to the gland assembly 30 upwards, and avoid the temperature rise rate of the cover plate 22 being affected by the heat of the whole machine.

In one embodiment, the lifting mechanism 31 includes a rotating motor 311, a lead screw 312, a fisheye ball head 313, a connecting pin, and a fixing block 314; the top end of the screw 312 is connected to the output shaft of the rotating motor 311, and the bottom end of the screw is connected to the fisheye ball head 313; the connecting pin is sleeved on the fisheye ball head 313, and two ends of the connecting pin are fixed on the fixing block 314. Specifically, through the matching of the fisheye ball 313, the connecting pin and the fixing block 314, the rotary power output by the rotary motor 311 is converted into a linear motion driving force acting on the first pressing block 32, so that the lifting motion of the first pressing block 32 is realized, and the lifting device is simple and small in structure and stable in operation.

In one embodiment, the gland assembly 30 includes a transfer block 36, a plurality of second guide posts 37; the transfer block 36 is connected to the translation mechanism 50; the lifting mechanism 31 and the second guide post 37 are fixed to the transfer block 36 respectively; the first presser 32 is slidably connected to the second guide post 37. Specifically, the second guide pillar 37 is used for guiding and supporting, so as to improve the stability of the first press block 32 during lifting.

Further, the translation mechanism 50 includes:

two guide rails 51 fixed to the frame 10;

and two sliding blocks 52 fixed on the transfer block 36 and slidably connected to the matching guide rails 51. Specifically, the structure is simple and small through the sliding connection of the guide rail 51 and the slide block 52, and the pressing cover assembly 30 can be driven by external force to translate relative to the frame 10 so as to change the position of the pressing cover assembly 30 relative to the incubation assembly 20, thereby facilitating the loading or unloading of the PCR tube onto or from the heating seat 21.

In an embodiment, the sliding fit between the guide rail 51 and the sliding block 52 allows the pressing cover assembly 30 to be manually driven to translate, which is simple and convenient to operate without an additional driving mechanism, and is beneficial to the miniaturization design of the device body 100. In yet another embodiment, a translation drive is provided to drive the platen assembly 30 in translation, which is automated.

In one embodiment, the reading assembly 40 includes a fiber mounting plate 41, at least one light source 42, at least one detector 43, a plurality of incident excitation fibers 44, a plurality of detection collection fibers 45, and a light source fixing plate 46; the optical fiber mounting plate 41 is positioned between the incubation component 20 and the light source fixing plate 46 and is provided with a plurality of first through holes 411 and a plurality of second through holes 412; the light source 42 and the detector 43 are fixed on the light source fixing plate 46;

the card slot 211 is connected to the first through hole 411 and the second through hole 412 through the incident excitation fiber 44 and the detection collection fiber 45, respectively. Specifically, the card slot 211 is provided with a third through hole 2111 and a fourth through hole 2112; the number of the card slots 211, the first through holes 411 and the second through holes 412 is the same; each card slot 211 is connected to an incident excitation fiber 44 through a third through hole 2111 and to a detection collection fiber 45 through a fourth through hole 2112, so as to implement fiber-optic fluorescence detection. The number of light sources 42 may be one, two, three or even more to provide different colors of incident light. The number of the detectors 43 is the same as that of the light sources 42, so as to cooperate with the incident light excitation and the fluorescence signal receiving.

During detection, the light source 42 emits light out of the optical fiber, emits the light out of the first through hole 411, and transmits the light into the PCR tube in the corresponding card slot 211 through the incident excitation optical fiber 44 to excite the sample to be detected to generate fluorescence, and the generated fluorescence is transmitted to the corresponding detector 43 through the detection collection optical fiber 45 and the second through hole 412, so that detection is finally achieved.

Further, a plurality of first through holes 411 are arranged in a circumferential array to form an outer ring hole structure; the second through holes 412 are arranged in a circumferential array to form an inner ring hole structure; the inner ring hole structure is positioned in the outer ring hole structure;

an annular clamping groove 413 is formed in the back of the optical fiber mounting plate 41, and the annular clamping groove 413 is arranged around the outer side of the inner ring hole structure; the front surface of the light source fixing plate 46 is provided with an annular protrusion 461, and the light source 42 and the detector 43 are respectively located on the outer side and the inner side of the annular protrusion 461; the annular protrusion 461 is engaged with the annular engaging groove 413. Specifically, by designing the arrangement structure of the first through holes 411 and the second through holes 412 and designing the clamping structure of the optical fiber mounting plate 41 and the light source fixing plate 46, the environment of the detector 43 is sealed, so that the light emitted by the light source 42 is prevented from interfering with the detection of the detector 43, the reading is stable, and the problem of fluorescence crosstalk is solved.

Further, the reading assembly 40 further comprises a rotary driving member 47, a reading circuit board 48; the output shaft of the rotary driving member 47 is coaxially connected to the light source fixing plate 46 to switch the incident light output from the first through hole 411; the reading circuit board 48 is fixed to the back of the light source fixing plate 46. Specifically, the light source fixing plate 46 is driven to rotate by the rotary driving member 47 to switch the light source 42 corresponding to the first through hole 411 and switch the detector 43 corresponding to the second through hole 412, so as to realize fluorescence detection of different wavelengths. The reading circuit board 48 is arranged on the back of the light source fixing plate 46, the length of the wire harness of the electrical connection is reduced, when the rotary driving piece 47 runs, the fluorescent detection is completed by one turn at different wavelengths, and after the detection is completed, the turn is reset to ensure that the circuit winding is avoided while the continuous detection is performed. In addition, the device body 100 includes two power drivers, namely, the rotating motor 311 and the rotating driver 47, which is efficient in detection and compact in structure.

In one embodiment, the rotary driving member 47 is a synchronous belt driving mechanism, which has a small structure and stable driving.

In one embodiment, the optical coupler 491 is disposed on the back surface of the optical fiber mounting plate 41, and the stop piece 492 is disposed on the front surface of the light source fixing plate 46 to obtain the rotation position of the light source fixing plate 46.

In one embodiment, the light source 42 is an LED lamp.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way; the utility model can be smoothly implemented by the ordinary technicians in the industry according to the drawings and the above description; however, those skilled in the art should understand that changes, modifications and variations made by the above-described technology can be made without departing from the scope of the present invention, and all such changes, modifications and variations are equivalent embodiments of the present invention; meanwhile, any changes, modifications, evolutions, etc. of equivalent changes made to the above embodiments according to the essential technology of the present invention still belong to the protection scope of the technical solution of the present invention.

Claims (10)

1. A PCR amplification detection apparatus comprising:

a frame (10) to form a support structure;

an incubation assembly (20), fixed to the frame (10), comprising a heating seat (21), a cover plate (22), a heating element (23); the heating seat (21) is convexly provided with a plurality of clamping grooves (211) for accommodating the PCR tube and heating the PCR tube; the cover plate (22) is covered above the heating seat (21); the heating element (23) is mounted on at least one surface of the cover plate (22) to heat the cover plate (22);

the gland assembly (30) is arranged above the incubation assembly (20) and comprises a lifting mechanism (31), a first pressing block (32), a second pressing block (33), a plurality of first guide pillars (34) and a plurality of springs; the driving end of the lifting mechanism (31) is connected to the first pressing block (32) and used for driving the first pressing block (32) to move up and down; the top end of the first guide pillar (34) is connected with the first pressing block (32) in a sliding mode, and the bottom end of the first guide pillar is fixed to the second pressing block (33); the spring is sleeved on the first guide post (34) and used for buffering the pressing force of the first pressing block (32);

the reading component (40) is used for reading the fluorescence information of the object to be detected in the PCR tube;

a translation mechanism (50) fixed to the rack (10) for translating the gland assembly (30) to allow the heating block (21) to load or reposition the PCR tube;

under the driving of the lifting mechanism (31), the first pressing block (32) presses the second pressing block (33) through the spring, so that the second pressing block (33) presses the cover plate (22), and the cover plate (22) covers the heating seat (21) and abuts against the top cover of the PCR tube.

2. The PCR amplification detection apparatus according to claim 1, further comprising a heat dissipation assembly (60) disposed below the incubation assembly (20).

3. The PCR amplification detection apparatus according to claim 2, wherein the heat dissipation assembly (60) comprises a heat sink structure (61), a fan (62); the heat sink structure (61) is disposed proximate to the incubation assembly (20).

4. The PCR amplification detecting apparatus according to claim 1, wherein a heat insulating member (35) is provided under the second pressing block (33).

5. The PCR amplification detection device according to claim 1, wherein the lifting mechanism (31) comprises a rotating motor (311), a screw rod (312), a fisheye ball head (313), a connecting pin and a fixing block (314); the top end of the screw rod (312) is connected to an output shaft of the rotating motor (311), and the bottom end of the screw rod is connected to the fisheye ball head (313); the connecting pin is sleeved on the fisheye ball head (313), and two ends of the connecting pin are fixed on the fixing block (314).

6. The PCR amplification detecting apparatus according to any one of claims 1 to 5, wherein the gland assembly (30) comprises a transfer block (36), a plurality of second guide pillars (37); the transfer block (36) is connected to the translation mechanism (50); the lifting mechanism (31) and the second guide post (37) are respectively fixed on the transfer block (36); the first pressing block (32) is connected to the second guide pillar (37) in a sliding manner.

7. The PCR amplification detecting apparatus according to claim 6, wherein the translation mechanism (50) comprises:

two guide rails (51) fixed to the frame (10);

and the two sliding blocks (52) are fixed on the transfer block (36) and are connected with the matched guide rails (51) in a sliding manner.

8. The PCR amplification detecting apparatus according to claim 1, wherein the reading assembly (40) comprises a fiber mounting plate (41), at least one light source (42), at least one detector (43), a plurality of incident excitation fibers (44), a plurality of detection collecting fibers (45), and a light source fixing plate (46); the optical fiber mounting plate (41) is positioned between the incubation component (20) and the light source fixing plate (46) and is provided with a plurality of first through holes (411) and a plurality of second through holes (412); the light source (42) and the detector (43) are fixed on the light source fixing plate (46);

the clamping groove (211) is respectively connected with the first through hole (411) and the second through hole (412) through the incident excitation optical fiber (44) and the detection collection optical fiber (45).

9. The PCR amplification detecting device of claim 8, wherein the first through holes (411) are arranged in a circumferential array to form an outer ring hole structure; the second through holes (412) are arranged in a circumferential array to form an inner ring hole structure; the inner ring hole structure is positioned in the outer ring hole structure;

an annular clamping groove (413) is formed in the back face of the optical fiber mounting plate (41), and the annular clamping groove (413) is arranged around the outer side of the inner ring hole structure; the front surface of the light source fixing plate (46) is provided with an annular bulge (461), and the light source (42) and the detector (43) are respectively positioned on the outer side and the inner side of the annular bulge (461); the annular protrusion (461) is clamped in the annular clamping groove (413).

10. The PCR amplification detection apparatus according to claim 8, wherein the reading assembly (40) further comprises a rotary driving member (47), a reading circuit board (48); the output shaft of the rotary driving piece (47) is coaxially connected to the light source fixing plate (46); the reading circuit board (48) is fixed on the back of the light source fixing plate (46).

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