CN217829588U - Simple antigen concentrator - Google Patents

Abstract

The utility model discloses a simple and easy antigen concentrator, this concentrator is including taking open-ended container (1), a serial communication port, the opening part is fit with the first lid of detachable (2), first lid (2) including taking open-ended installation department (21) and cover in dialysis membrane (22) on the opening of installation department (21), the opening of container (1) with the open position of installation department (21) is relative, still be equipped with bleeder vent (3) on container (1), aseptic filter membrane has been covered on bleeder vent (3). The utility model discloses simple structure, it is simple and convenient to use, can be in sterile condition all the time in the container, the antigen of results also is in the container always, is difficult to contact with the buffer solution, is favorable to protein and virus stability.

Description

Simple antigen concentrator

Technical Field

The utility model relates to a enrichment facility specifically relates to a simple and easy antigen concentrator.

Background

Concentration is the process of changing low concentration solution into high concentration solution by removing solvent (including water), and in the preparation of macromolecules and various biochemical products, concentration is often carried out after extraction and before crystallization, and sometimes throughout the whole preparation process.

At present, methods such as ultrafiltration membrane package concentration or ammonium persulfate concentration and dialysis bag concentration are mostly adopted for protein or virus concentration. The concentration method of the ultrafiltration concentration membrane can not ensure sterility, the concentration volume is at least 2L, the volume of the residual concentrated solution in a pipeline can not be measured, more equipment is needed, and the process is complex; the ammonium persulfate precipitation concentration method has a concentration effect on most of proteins and viruses, but the harvested antigens are often in a PBS buffer solution or other buffer solutions, the concentration efficiency by using ammonium persulfate is not high, protein loss exists in concentrated waste liquid, and the concentrated virus liquid is easy to inactivate; the dialysis bag concentration method can not ensure the aseptic operation, and is difficult to estimate the concentration multiple, which affects the subsequent experiment and causes great loss, in addition, the PEG (polyethylene glycol) is easy to mix into the concentrated solution when the dialysis bag is disassembled to absorb the antigen after dialysis.

SUMMERY OF THE UTILITY MODEL

To the many problems problem of above-mentioned concentration method, the utility model provides a simple and easy antigen concentrator, this concentrator simple structure, it is easy and simple to handle, the antigen of results is in the container, is difficult to contact with the buffer solution, is favorable to protein and virus to be stable.

In order to realize the above-mentioned purpose, the utility model provides a simple and easy antigen concentrator, this concentrator is including taking the open-ended container, the opening part adaptation has the first lid of detachable, first lid including take the open-ended installation department with cover in dialysis membrane on the opening of installation department, the opening of container with the open position of installation department is relative, still be equipped with the bleeder vent on the container, aseptic filter membrane has been covered on the bleeder vent.

Preferably, the mounting portion is threadedly coupled to the opening of the container.

Preferably, the mounting portion is externally provided with a projection.

Preferably, the dialysis membrane is pressed against the inside of the mounting portion through a sealing ring.

The pore diameter of the sterile filter membrane is less than 0.22 micron.

Preferably, a bacteria filter is connected to the air vent, and the sterile filter membrane is positioned in the bacteria filter.

Further, the bacterial filter is mounted on the second cover body.

Further, the second cover body is connected with the container through threads.

Preferably, the container is provided with a scale.

Preferably, a bracket is arranged outside the mounting part.

Through the technical scheme, the utility model discloses a following beneficial effect:

1. the utility model discloses simple structure, it is simple and convenient to use, can be in sterile condition all the time in the container, and the antigen of results also is in the container always, is difficult to contact with the buffer solution, is favorable to protein and virus stability.

2. The utility model discloses an among the preferred technical scheme, the dialysis membrane is split type with the installation department to support in the installation department through the sealing washer, can conveniently change the dialysis membrane, all the other parts used repeatedly, reduce cost.

3. In another preferred technical scheme of the utility model, first lid peripheral hardware support to it is stable to place when guaranteeing the concentrator inversion.

Drawings

FIG. 1 is a schematic diagram of a preferred embodiment of the antigen concentrator of the present invention;

fig. 2 is a schematic structural view of the first cover of the present invention;

fig. 3 is an exploded view of the first cover of the present invention.

Description of the reference numerals

1, a container;

2, a first cover body, 21 mounting parts, 211, a ring body, 212 connecting ribs, 22 dialysis membranes and 23 sealing rings;

3, air holes and 31 bacteria filters;

4 second cover body.

Detailed Description

The following describes in detail embodiments of the present invention with reference to the accompanying drawings. It is to be understood that the description of the embodiments herein is for purposes of illustration and explanation only and is not intended to limit the invention.

It should be noted that, in the following description, directional terms such as "outer" and "inner" are used to clearly describe the technical solution of the present invention, and all the directional terms refer to the directions of the components of the concentrator, for example, the portion where the liquid passes through is the inner portion, and the portion opposite to the inner portion is the outer portion. The term "part" is used merely to facilitate the description of the invention and to simplify the description, and does not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated, therefore, the features defined "first" and "second" may explicitly or implicitly include one or more of the features described.

In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; they may be connected directly or indirectly through intervening media, or they may be connected through inter-element communication or interaction between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

As shown in figure 1, simple and easy antigen concentrator including taking open-ended container 1, the opening part is adapted with the first lid 2 of detachable, first lid 2 including take open-ended installation department 21 with cover in dialysis membrane 22 on the opening of installation department 21, container 1's opening with the opening position of installation department 21 is relative, still be equipped with bleeder vent 3 on the container 1, aseptic filter membrane has been covered on bleeder vent 3.

The utility model discloses when using, will treat concentrated antigen in the aseptic environment pour into container 1 from the opening part of container 1 in, then invert container 1 (first lid 2 is down promptly) in the container that is equipped with aseptic PEG, the dialysis of stewing. After dialysis, the PEG at the 2 end of the first cover body is cleaned, the PEG flows into the antigen after the cover is opened, and the concentrated antigen is collected aseptically.

In the concentration process, because the solvent and the like in the container 1 can be gradually discharged out of the container 1, negative pressure is generated in the container 1 to influence the concentration process, and therefore, the air holes 3 are arranged to ensure that no pressure difference is kept between the inside and the outside of the container 1 as far as possible, so that the solvent and the like can be smoothly discharged out of the container 1. In order to avoid bacteria in the environment from entering the container 1, the sterile filter membrane is suitable, and can filter the bacteria to prevent the bacteria from entering the container 1, so that the inside of the container 1 is always in a sterile state, and the antigen cannot be polluted.

The utility model discloses simple structure, it is simple and convenient to use, can be in sterile condition all the time in the container 1, and the antigen of results also is in the container 1 always, is difficult to contact with the buffer solution, is favorable to protein and virus stability.

The first cover body 2 is detachably matched at the opening of the container 1, and the use is convenient. The mounting part 21 and the opening of the container 1 can be clamped and connected through threads.

In the case of a screw connection, the mounting portion 21 is preferably externally provided with a projection to increase the friction during screwing to avoid slipping.

The dialysis membrane 22 can be fixed on the first cover 2 by glue or other adhesives, or can be connected with the first cover 2 in a split manner, specifically, as shown in fig. 3, the dialysis membrane 22 is supported in the mounting portion 21 by a sealing ring 23. Under the condition of split type connection, assemble dialysis membrane 22 and installation department 21 before using, specifically, select for use the area to be greater than the dialysis membrane 22 of installation department 21 opening, put dialysis membrane 22 in installation department 21, cover the opening, then put into sealing washer 23 wherein, install installation department 21 at the opening part of container 1 again, sealing washer 23 seals first lid 2 and container 1 to make dialysis membrane 22 firmly at the opening part of installation department 21. In this case, the dialysis membrane 22 can be replaced conveniently, and the rest components can be reused, thereby reducing the cost.

The pore size of the sterile filter is preferably less than 0.22 micron. The bacteria are generally larger than 0.22 micron in diameter, so that by using a sterile filter membrane with a pore size smaller than 0.22 micron, the bacteria are blocked and cannot enter the container 1 when air passes through, and then the air entering the container 1 is sterile, so as to ensure the sterility of the container 1.

Further, for convenience of use, a commercially available product may be selected, for example, a bacterial filter 31 is connected to the air-permeable hole 3, and the sterile filter membrane is located in the bacterial filter 31. The bacterial filter 31 is mature and is available in the market, and then the installed end of the bacterial filter is connected to the air vent 3.

Preferably, the bacterial filter 31 is mounted on the second cover 4.

Further, the second cover 4 is screwed to the container 1.

Scales are arranged on the container 1 so as to be convenient for observing the amount of the liquid in the container 1 and judging the concentration process. The time required for dialysis can also be calculated from the volume of the liquid to be concentrated.

The utility model discloses first lid 2 is down when using, places stably in order to guarantee the concentrator, can suitably increase the area of first lid 2 tip, also can establish the support on first lid 2, specifically, as shown in fig. 1-fig. 3, installation department 21 is equipped with the support outward, and this support includes ring body 211 and splice bar 212, ring body 211 at least with the tip parallel and level of first lid 2, perhaps surpass the tip of first lid 2, the outside of ring body 211 and installation department 21 is connected to splice bar 212. The ring 211 supports the first cover 2 and the entire container 1 during the concentration process.

The following is a preferred embodiment of the present invention.

The concentrator comprises a container 1, a hollow first cover body 2 positioned at one end of the container 1, a second cover body 4 positioned at the other end of the container 1, a vent pipe arranged on the second cover body 4, and a bacteria filter 31 connected to the vent pipe.

It was used for Pala (18 KD) protein concentration using the following method:

1. selecting a dialysis bag with the volume of below 18KD/3, cutting one section, cutting from one side, placing the inner side on the hollow first cover body 2, and padding a sealing ring 23. Lightly covering on a suitable container 1 (a blue-cap bottle with a suitable volume and a marked volume (an inverted volume) can be selected), and covering the other end with a second cover body 4;

2. the second cover body 4 is opened, a proper amount of purified water is added, and whether the liquid leaks from one end of the dialysis bag is checked;

3. placing a little purified water (or ddw) in a beaker, placing one end of a first cover body 2 in the beaker to submerge the dialysis bag by water, unscrewing a second cover body 4, and screwing the second cover body 4 after autoclaving (one end of the dialysis bag is always screwed after the cover is arranged on the dialysis bag for leakage detection, and leakage detection needs to be carried out again once the cover is unscrewed);

4. cooling to room temperature, and detecting leakage again (one end of the dialysis bag faces downwards, so that liquid leakage does not occur);

5. opening the second cover body 4 in the super clean bench, pouring out the water in the container 1, adding the antigen, screwing the second cover body 4, putting the end of the first cover body 2 downwards into a beaker filled with sterile PEG, marking the position of the initial liquid level, standing and dialyzing for 6-8 h at 2-8 ℃, adding a small amount of water into the PEG in advance to dissolve part of the PEG to enable the PEG to be in a semi-liquid state, and facilitating the contact with a dialysis bag;

6. the time required for dialysis is calculated according to 20ml per hour of dialysis, the PEG on the first cover body 2 is cleaned after the end, the antigen is prevented from flowing into the antigen after the cover is opened, and the concentrated antigen is aseptically collected.

The preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, however, the present invention is not limited to the details of the above embodiments, and the technical concept of the present invention can be within the scope of the present invention to perform various simple modifications to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and in order to avoid unnecessary repetition, the present invention does not separately describe various possible combinations.

In addition, various embodiments of the present invention can be combined arbitrarily, and the disclosed content should be regarded as the present invention as long as it does not violate the idea of the present invention.

Claims (10)

1. The utility model provides a simple and easy antigen concentrator, is including taking open-ended container (1), its characterized in that, the opening part adapter has the first lid of detachable (2), first lid (2) including take open-ended installation department (21) and cover in dialysis membrane (22) on the opening of installation department (21), the opening of container (1) with the open position of installation department (21) is relative, still be equipped with bleeder vent (3) on container (1), aseptic filter membrane has been covered on bleeder vent (3).

2. The simple antigen concentrator of claim 1, wherein the mounting portion (21) is threadedly connected to an opening of the container (1).

3. The simple antigen concentrator of claim 2, wherein the mounting portion (21) is externally provided with a protrusion.

4. The simple antigen concentrator as claimed in claim 1, wherein the dialysis membrane (22) is pressed against the inside of the mounting portion (21) by a sealing ring (23).

5. The simple antigen concentrator of claim 1, wherein the pore size of the sterile filter membrane is less than 0.22 μm.

6. The simple antigen concentrator of claim 1, wherein the air hole (3) is connected with a bacterial filter (31), and the sterile filter membrane is positioned in the bacterial filter (31).

7. The simple antigen concentrator of claim 6, wherein the bacterial filter (31) is mounted on the second cover (4).

8. The simple antigen concentrator of claim 7, wherein the second cover (4) is screwed to the container (1).

9. The simple antigen concentrator as claimed in one of claims 1 to 8, characterized in that the container (1) is provided with a scale.

10. The simple antigen concentrator of any one of claims 1 to 8, wherein a bracket is provided outside the mounting portion (21).

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