CN217739001U - Microscopic imaging detection system - Google Patents

Abstract

The utility model discloses a micro-imaging detecting system for carry out the formation of image to the sample and detect, micro-imaging detecting system includes: the imaging detection device is used for bearing a sample and carrying out optical excitation operation on the sample to obtain a target optical signal; the acquisition detection module is coupled with the imaging detection device and is used for generating a detection result according to the target optical signal; wherein, formation of image detection device includes: the light source module is used for generating an initial light signal and controlling the initial light signal to emit at a preset angle; wherein the preset angle is less than 90 degrees; the bearing piece is coupled with the light source module and used for bearing a sample; wherein, the sample is used for generating a fluorescence optical signal according to the excitation of the initial optical signal; and the imaging module is coupled with the bearing part and used for generating a target optical signal according to the fluorescent optical signal. The utility model discloses a microscopic imaging detecting system can improve sample detection's efficiency.

Description

Microscopic imaging detection system

Technical Field

The utility model relates to a micro-imaging technology field especially relates to a micro-imaging detecting system.

Background

At present, the diffusion characteristics of a substance to be measured can be known by detecting the substance as a sample.

However, in the related art, the sample is usually required to be amplified and cultured first to detect the substance to be detected in the sample, and therefore, the detection method has low detection efficiency.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a micro-imaging detecting system can improve the efficiency that the sample detected to a certain extent.

The application provides a microscopic imaging detection system, microscopic imaging detection system is used for carrying out the formation of image to the sample and detects, microscopic imaging detection system includes: the imaging detection device is used for bearing the sample and carrying out optical excitation operation on the sample to obtain a target optical signal; the acquisition detection module is coupled with the imaging detection device and is used for generating a detection result according to the target optical signal; wherein the imaging detection apparatus comprises: the light source module is used for generating an initial light signal emitted at a first preset angle; wherein the first preset angle is less than 90 degrees; the bearing piece is coupled with the light source module and used for bearing the sample; wherein the sample is used for generating a fluorescent light signal according to the initial light signal excitation; an imaging module coupled to the carrier, the imaging module configured to generate the target light signal according to the fluorescent light signal.

The microscopic imaging detection system of this embodiment carries out light excitation operation and formation of image to the sample through formation of image detection device to obtain target light signal, and gather the detection to target light signal through gathering detection module, with the formation of detection result, thereby realize high-efficient accurate detection to the material that awaits measuring in the sample, and the microscopic imaging detection system's in this embodiment simple structure, the cost of manufacture is lower.

In some embodiments, the acquisition detection module comprises: the CMOS camera is used for collecting the target optical signal and generating an image signal according to the target optical signal; a calculation unit for generating the detection result from the image signal.

In some embodiments, the microscopic imaging detection system further comprises: the control module is respectively connected with the bearing piece, the imaging module and the CMOS camera and is used for generating a first control signal and a second control signal according to the image signal; wherein the carrier is further configured to move in accordance with the first control signal such that the sample receives the initial light signal; the imaging module is further configured to move according to the second control signal to adjust a focal plane of the imaging module.

In some embodiments, the control module comprises: the first motor is respectively connected with the CMOS camera and the bearing piece, and is used for controlling the bearing piece to move according to the first control signal; and the second motor is respectively connected with the CMOS camera and the imaging module and is used for controlling the imaging module to move according to the second control signal.

In some embodiments, the control module further comprises: the pixel calculation unit is connected with the CMOS camera and used for generating a gray variance value according to the image signal; and the processing unit is respectively connected with the pixel calculation unit, the first motor and the second motor, and is used for generating the first control signal and the second control signal according to the gray variance value.

In some embodiments, the light source module includes: an LED light source for generating a laser signal; the oblique incidence unit is connected with the LED light source and used for generating the initial light signal emitted at the preset angle according to the laser signal.

In some embodiments, the oblique incidence unit includes: the aspheric lens is coupled with the LED light source; a first attenuation sheet coupled to the aspheric lens; a second attenuation sheet coupled with the first attenuation sheet; the first optical filter is coupled with the second attenuation sheet; the aspheric lens, the first attenuation sheet, the second attenuation sheet and the first optical filter are all used for modulating the laser signal; the dichroic mirror is coupled and connected with the first optical filter at a second preset angle, and the second preset angle is smaller than 90 degrees; the dichroic mirror is used for reflecting the modulated laser signal to obtain a reflection signal; an achromatic lens; the achromatic lens and the dichroic mirror are in coupling connection at a third preset angle, and the third preset angle is smaller than 90 degrees; the achromatic lens is used for processing the reflection signal to obtain the initial optical signal.

In some embodiments, the imaging module comprises: a tri-cemented lens coupled with the carrier; the second optical filter is coupled with the three cemented lenses; the tri-cemented lens and the second filter are used for modulating the fluorescent light signal; and the imaging lens is coupled with the second optical filter and is used for focusing the fluorescent light signal to obtain the target light signal.

In some embodiments, the microscopic imaging detection system further comprises: a housing for sealing the imaging detection device; wherein, the material of casing is light-tight material.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The invention will be further described with reference to the following drawings and examples, in which:

FIG. 1 is a schematic diagram of a frame of the microscopic imaging detection system of the present invention;

fig. 2 is another schematic diagram of the framework of the microscopic imaging detection system of the present invention;

FIG. 3 is a schematic diagram of another embodiment of the microscopic imaging detection system of the present invention;

FIG. 4 is a schematic diagram of another embodiment of the microscopic imaging detection system of the present invention;

fig. 5 is a schematic view of a light path of the microscopic imaging detection system of the present invention.

Reference numerals: the microscopic imaging detection system 100, the imaging detection device 110, the light source module 111, the bearing 112, the imaging module 113, the acquisition detection module 120, the CMOS camera 121, the calculation unit 122, the control module 130, the first motor 131, the second motor 132, the pixel calculation unit 134, the processing unit 135, the LED light source 140, the aspheric lens 141, the first attenuation sheet 142, the second attenuation sheet 143, the first optical filter 144, the dichroic mirror 145, the achromatic lens 146, the cemented triplet 147, the second optical filter 148, and the imaging lens 149.

Detailed Description

Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that the directional descriptions, such as the directions or positional relationships indicated by upper, lower, front, rear, left, right, etc., are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but not for indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means is one or more, a plurality of means is two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the number, and the terms greater than, less than, within, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

In the description of the present invention, reference to the description of the terms "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The substance to be measured includes various substances, and the present application takes a harmful substance that is harmful to food safety as an example. In the related technologies, the detection of harmful substances in food still has the problems of tedious process, low efficiency and the like, and the related technologies comprise enzyme-linked immunosorbent assay, polymerase chain reaction, loop-mediated isothermal amplification, denaturing high performance liquid chromatography, biochip technology and the like. The enzyme-linked immunosorbent assay uses harmful substances as antigens, and the antigens can be combined with specific antibodies to generate specific binding reaction. However, enzyme-linked immunosorbent assay has high requirements for temperature and operation method, so that detection result errors are easily caused, and the experimental repeatability is poor. In addition, the polymerase chain reaction and the loop-mediated isothermal amplification technology require amplification of a sample to obtain a desired detection signal, and only a quantitative sample can be detected, so that efficient detection cannot be performed. The development period of the biochip technology is long, and the preparation cost is high.

Therefore, the application provides a microscopic imaging detection system which is simple in structure, low in price and capable of improving the detection efficiency and the detection accuracy of a sample.

Referring to fig. 1, the present application provides a microscopic imaging detection system 100, the microscopic imaging detection system 100 is used for performing imaging detection on a sample, the microscopic imaging detection system 100 includes: an imaging detection device 110, wherein the imaging detection device 110 is used for carrying the sample and performing a light excitation operation on the sample to obtain a target light signal; the acquisition detection module 120, the acquisition detection module 120 is coupled to the imaging detection apparatus 110, and the acquisition detection module 120 is configured to generate a detection result according to the target optical signal; wherein the imaging detection apparatus 110 comprises: the light source module 111 generates an initial light signal emitted at a first preset angle; wherein the first preset angle is less than 90 degrees; wherein the preset angle is less than 90 degrees; a carrier 112, wherein the carrier 112 is coupled to the light source module 111, and the carrier 112 is used for carrying the sample; wherein the sample is used for generating a fluorescent light signal according to the initial light signal excitation; an imaging module 113, wherein the imaging module 113 is coupled to the carrier 112, and the imaging module 113 is configured to generate a target light signal according to the fluorescent light signal.

It will be appreciated that luminescent bacteria are included in the sample and the sample is placed in the imaging detection device 110. When the imaging detection apparatus 110 performs a light excitation operation on the sample, the luminescent bacteria in the sample can be affected by the light excitation operation, and a luminescent reaction can be generated. The luminescent response of the luminescent bacteria is very sensitive to external stimuli, and any factor interfering or damaging the physiological process of the luminescent bacteria can change the luminescent intensity of the luminescent bacteria. Therefore, when a harmful substance comes into contact with the luminescent bacteria in the sample, the harmful substance has an effect of suppressing luminescence of the luminescent bacteria, so that the luminescent bacteria change in luminous intensity under the influence of the light excitation operation, and the luminous intensity thereof decreases as the concentration of the harmful substance increases. Therefore, the luminescence intensity of the luminescent bacteria can be used as an index for detecting a harmful substance.

It can be understood that the microscopic imaging detection system 100 in this embodiment includes an imaging detection apparatus 110 and an acquisition detection module 120. Specifically, the imaging detection device 110 includes a light source module 111, a carrier 112, and an imaging module 113, where the light source module 111 and the carrier 112 are coupled, and the imaging module 113 and the carrier 112 are coupled. The acquisition detection module 120 is coupled to the imaging module 113 in the imaging detection apparatus 110.

It is understood that the carrier 112 is used for carrying a sample, and the light source module 111 is used for generating an initial light signal and controlling the initial light signal to irradiate the sample at a preset angle. Wherein the preset angle is less than 90 degrees. The luminescent bacteria in the sample produce luminescent reaction under the excitation of the initial light signal, and can generate a fluorescent light signal with certain luminous intensity. From the above, the fluorescent light signal can represent the presence and concentration of the harmful substance in the sample.

As can be appreciated, the imaging module 113 can receive the fluorescent light signal generated by the sample under excitation of the initial light signal and image the fluorescent light signal to obtain the target light signal. When the sample contains harmful substances, the target optical signal can be obviously changed, and particularly, the light intensity of the target optical signal can reflect the concentration condition of the harmful substances in the sample.

As can be understood, the imaging module 113 is configured to receive the target optical signal generated by the imaging module 113, collect and detect the target optical signal to determine whether there is a harmful substance in the sample, detect the light intensity of the target optical signal to determine the concentration of the harmful substance, and generate a corresponding detection result.

The microscopic imaging detection system 100 of this embodiment performs optical excitation operation and imaging on a sample through the imaging detection device 110 to obtain a target optical signal, and collects and detects the target optical signal through the collection detection module 120 to generate a detection result, so as to realize efficient and accurate detection on a substance to be detected in the sample, and the microscopic imaging detection system 100 of this embodiment has a simple structure and a low preparation cost.

Referring to fig. 2, in some embodiments, the acquisition detection module 120 includes: a CMOS camera 121, wherein the CMOS camera 121 is configured to collect the target light signal and generate an image signal according to the target light signal; a computing unit 122, the computing unit 122 being further configured to generate the detection result according to the image signal.

As can be appreciated, the CMOS camera 121 is coupled to the imaging module 113. The imaging module 113 generates a target light signal and focuses the target light signal onto the lens of the CMOS camera 121. The CMOS camera 121 receives the target light signal and collects the target light signal to generate a corresponding image signal. The calculation unit 122 processes the image signal to generate a detection result.

Specifically, the calculation unit 122 imports the image signal, and extracts and analyzes the fluorescence light intensity signal contained therein to obtain a data file of fluorescence light intensity information. In addition, the data file is subjected to operations such as counting, light intensity signal curve and relative concentration calculation, and finally a corresponding detection result is generated. The above method for generating the detection result according to the image signal can be implemented by referring to the related art, and this is not specifically described and limited in the embodiments of the present application.

It is understood that the microscopic imaging detection system 100 in the present embodiment may further include a display module. The display module is connected with the CMOS camera 121, and can receive and display the image signal generated by the CMOS camera 121, so that the experimenter can conveniently check the imaging condition of the sample.

It can be understood that, compared with the expensive CCD camera used for detecting the sample in the related art, the microscopic imaging detection system 100 of the present embodiment combines the imaging module 113 with the luminescence reaction based on the luminescent bacteria to perform the focused imaging on the target light signal excited by the sample, and the target light signal can be collected and generated into the corresponding image signal by the simple CMOS camera 121. For example, in one particular embodiment, a smartphone is used as the acquisition detection module. Specifically, the lens of the smart phone is used to collect the target light signal generated by the imaging module 113 and generate an image signal. The smart phone is provided with a corresponding calculating unit which can calculate and display the image signal. In addition, the smart phone can also transmit the image signal to computer equipment through a wireless network for processing so as to obtain a detection result.

From the above, the microscopic imaging detection system 100 of the present embodiment has the advantages of portability, fast detection, and low preparation cost, and can be flexibly applied to various detection scenes. Among them, it is particularly suitable for the field of microscopic imaging.

Referring again to fig. 2, in some embodiments, the microscopic imaging detection system 100 further comprises: a control module 130, wherein the control module 130 is respectively connected to the carrier 112, the imaging module 113 and the CMOS camera 121, and the control module 130 is configured to generate a first control signal and a second control signal according to the image signal; wherein the carrier 112 is further configured to move according to the first control signal so that the sample receives the initial light signal; the imaging module 113 is further configured to move according to the second control signal to adjust a focal plane of the imaging module 113.

As can be appreciated, the control module 130 is connected to the CMOS camera 121 for receiving an image signal generated by the CMOS camera 121 according to the target light signal. Specifically, since the focal plane of the imaging module 113 changes due to the change of the object plane, the optimal focal plane for generating the target light signal also changes when different samples or different regions of the samples are focused and imaged by the imaging module 113. In order to enable the CMOS camera 121 to obtain the target light signal on the optimal focal plane after imaging different samples or different regions of the samples, so as to generate a clear image, the control module 130 controls the imaging module 113 to change the focal plane position of the imaging module 113. Specifically, the control module 130 receives and processes the image signal to determine whether the image signal is clear, and generates a corresponding first control signal. The imaging module 113 moves up and down according to the first control signal to change the position of its focal plane, so that the CMOS camera 121 can receive the target light signal on the focal plane, thereby obtaining a clear image signal.

It will be appreciated that the control module 130 is also adapted to interface with the carrier 112. When the microscopic imaging detection system 100 needs to detect multiple samples of a sample set, the carrier 112 in this embodiment can be used to simultaneously carry multiple samples for efficient automatic detection. The control module 130 can control the bearing 112 to move left and right, so that the microscopic imaging detection system 100 can perform imaging detection on different samples. Specifically, as can be seen from the above, when the control module 130 receives the image signal, the control module 130 processes the image signal to determine whether the image signal is clear. If the image signal is not clear, the control module 130 generates a corresponding first control signal to adjust the focal plane of the imaging module 113. At this time, since the microscopic imaging detection system 100 has not acquired a clear image signal, the imaging acquisition needs to be performed again after the adjustment of the imaging module 113, and therefore, the carrier 112 needs to be kept still. If the image signal is clear, the carrier 112 can move to the next sample for inspection. At this time, the control module 130 generates a corresponding second control signal according to which the carrier 112 moves, thereby realizing automatic detection of the sample group.

Referring to fig. 3, in some embodiments, the control module 130 includes: a first motor 131, wherein the first motor 131 is respectively connected with the CMOS camera 121 and the carrier 112, and the first motor 131 is used for controlling the carrier 112 to move according to the first control signal; and a second motor 132, wherein the second motor 132 is respectively connected to the CMOS camera 121 and the imaging module 113, and the second motor 132 is configured to control the imaging module 113 to move according to the second control signal.

Specifically, the first motor 131 and the second motor 132 may be stepping motors. The first motor 131 can control the bearing part 112 precisely, and the precision can reach micron order. Therefore, when the microscopic imaging detection system 100 detects a sample, the first motor 131 can control the bearing member 112 to move in a micrometer range, so as to realize accurate detection of different areas of the sample.

Specifically, the second motor 132 is used to adjust the imaging module 113 so that the CMOS camera 121 obtains a sample image taken at the best focal plane.

Referring to fig. 4, in some embodiments, the control module 130 further includes: a pixel calculation unit 134, wherein the pixel calculation unit 134 is connected to the CMOS camera 121, and the pixel calculation unit 134 is configured to generate a gray scale variance value according to the image signal; a processing unit 135, wherein the processing unit 135 is respectively connected to the pixel calculating unit 134, the first motor 131 and the second motor 132, and the processing unit 135 is configured to generate the first control signal and the second control signal according to the gray variance value.

It can be understood from the above description that the control module 130 can receive the image signal and process the image signal to determine whether the image signal is clear. Specifically, the definition of the image signal may be represented by a gray scale variance value, and the clearer the image signal is, the larger the contrast of the image is, the larger the gray scale variance value in the image signal is, that is, the larger the difference between the gray scale values of the pixels of the image signal is. Therefore, the gray-scale values of all the pixels in the image signal are calculated by the pixel calculating unit 134 to obtain the gray-scale variance value. The pixel calculation unit 134 is connected to the processing unit 135, and transmits the gray variance value to the processing unit 135. A preset threshold value is preset in the processing unit 135, and the preset threshold value is used for representing the minimum value of the gray variance value when the image signal is clear. The processing unit 135 calculates the gray variance value according to a preset threshold to determine whether the corresponding image signal is clear, so that the control module 130 determines whether to move the carrier 112 and the imaging module 113.

Referring to fig. 5, in some embodiments, the light source module 111 includes: an LED light source 140, the LED light source 140 for generating a laser signal; and an oblique incidence unit connected to the LED light source 140, the oblique incidence unit being configured to generate the initial light signal emitted at the first preset angle according to the laser signal.

Specifically, the power supply of the LED light source 140 is a 5V battery power supply, the laser signal generated by the LED light source 140 may be 532nm laser, and the power of the laser signal is 1W. The laser signal is incident into an oblique incidence unit coupled to the LED light source 140, and the oblique incidence unit generates a corresponding initial light signal according to the laser signal.

Referring again to fig. 5, in some embodiments, the oblique incidence unit includes: an aspheric lens 141, wherein the aspheric lens 141 is coupled to the LED light source 140; a first attenuation sheet 142, wherein the first attenuation sheet 142 is coupled to the aspheric lens 141; a second attenuation plate 143, wherein the second attenuation plate 143 is coupled with the first attenuation plate 142; a first filter 144, wherein the first filter 144 is coupled to the second attenuator 143; the aspheric lens 141, the first attenuation sheet 142, the second attenuation sheet 143, and the first optical filter 144 are all configured to modulate the laser signal; the dichroic mirror 145, the dichroic mirror 145 and the first optical filter 144 are coupled and connected at a second preset angle, and the second preset angle is smaller than 90 degrees; the dichroic mirror 145 is configured to reflect the modulated laser signal to obtain a reflection signal; an achromatic lens 146; the achromatic lens 146 and the dichroic mirror 145 are coupled at the third preset angle, and the third preset angle is smaller than 90 degrees; wherein, the achromatic lens 146 is configured to process the reflected signal to obtain the initial optical signal.

In particular, the laser signal generated by the LED light source 140 has a large divergence angle. Aspheric lens 141 in this embodiment is used for collimating the light path of the laser signal, and the collimated laser signal passes through first attenuation sheet 142 and second attenuation sheet 143 that are parallel to each other, and generates corresponding collimated monochromatic light after the first filter, so can effectively reduce the divergence angle, can prevent that sample fluorescence from bleaching too fast simultaneously. The collimated monochromatic light is then reflected by dichroic mirror 145 and focused by achromatic lens 146 to generate an initial optical signal. Wherein, the dichroic mirror 145 and the first filter are disposed at a predetermined angle, so that the collimated monochromatic light is reflected at the predetermined angle. The reflected collimated monochromatic light is focused by the achromatic lens 146 to generate an initial light signal, and the initial light signal is focused on the sample at the preset angle. The above-mentioned preset angle is preferably 75 degrees. When the initial light signal irradiates on the sample in a 75-degree oblique incidence mode, the influence of stray light can be eliminated, the signal-to-noise ratio can be effectively improved, and large flare can be avoided from being generated in the image signal. Therefore, the oblique incidence unit in the embodiment can improve the accuracy of microscopic imaging detection by generating the initial light signal according to the laser signal and controlling the initial light signal to be focused on the sample at a preset angle.

Referring again to fig. 5, in some embodiments, the imaging module 113 includes: a triplexer lens 147 coupled to the carrier 112; a second optical filter 148, wherein the second optical filter 148 is coupled to the triple cemented lens 147; wherein, the triple cemented lens 147 and the second filter 148 are both used for modulating the fluorescent light signal; an imaging lens 149, wherein the imaging lens 149 is coupled to the second optical filter 148, and the imaging lens 149 is configured to focus the fluorescent light signal to obtain the target light signal.

As can be understood, the sample is excited by the initial optical signal to generate a fluorescence optical signal, and the imaging module 113 images the fluorescence optical signal to generate a target optical signal. Specifically, the imaging module 113 includes a cemented triplet 147, a second filter 148, and an imaging lens 149. The tri-cemented lens 147 is used to collect the fluorescence light signal and eliminate the chromatic aberration generated during the imaging process, thereby improving the signal-to-noise ratio of the microscopic imaging detection system 100. Specifically, the focal length of the cemented triplet 147 may be 25mm. The second filter 148 is used to eliminate the noise generated by the original light signal, and only the fluorescence light signal is left, and the imaging lens 149 is used to image the fluorescence light signal to obtain the target light signal.

In some embodiments, the microscopic imaging detection system 100 further comprises: a housing for sealing the imaging detection apparatus 110; wherein, the material of casing is light tight material.

It is understood that the housing may be made of a resin material to ensure that the micro-imaging inspection system 100 is lightweight and portable. The casing made of the light-tight material can prevent an external light source from influencing the acquisition of the target light signal by the CMOS camera 121, so that the detection accuracy is improved.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

Claims (9)

1. A microscopic imaging detection system for performing imaging detection on a sample, the microscopic imaging detection system comprising:

the imaging detection device is used for bearing the sample and carrying out optical excitation operation on the sample to obtain a target optical signal;

the acquisition detection module is coupled with the imaging detection device and used for generating a detection result according to the target optical signal;

wherein the imaging detection apparatus comprises:

the light source module is used for generating an initial light signal emitted at a first preset angle; wherein the first preset angle is smaller than 90 degrees;

the bearing piece is coupled with the light source module and used for bearing the sample; wherein the sample is used for generating a fluorescent light signal according to the initial light signal excitation;

an imaging module coupled to the carrier, the imaging module configured to generate the target light signal according to the fluorescent light signal.

2. The microscopic imaging detection system according to claim 1, wherein the acquisition detection module comprises:

the CMOS camera is used for collecting the target optical signal and generating an image signal according to the target optical signal;

a calculation unit for generating the detection result from the image signal.

3. The microscopic imaging detection system according to claim 2, further comprising:

the control module is respectively connected with the bearing piece, the imaging module and the CMOS camera and is used for generating a first control signal and a second control signal according to the image signal;

wherein the carrier is further configured to move in accordance with the first control signal such that the sample receives the initial light signal; the imaging module is further configured to move according to the second control signal to adjust a focal plane of the imaging module.

4. The microscopic imaging detection system according to claim 3, wherein the control module comprises:

the first motor is respectively connected with the CMOS camera and the bearing piece, and is used for controlling the bearing piece to move according to the first control signal;

and the second motor is respectively connected with the CMOS camera and the imaging module and is used for controlling the imaging module to move according to the second control signal.

5. The microscopic imaging detection system according to claim 4, wherein the control module further comprises:

the pixel calculation unit is connected with the CMOS camera and used for generating a gray variance value according to the image signal;

and the processing unit is respectively connected with the pixel calculation unit, the first motor and the second motor, and is used for generating the first control signal and the second control signal according to the gray variance value.

6. The microscopic imaging detection system according to claim 1, wherein the light source module comprises:

an LED light source for generating a laser signal;

the oblique incidence unit is connected with the LED light source and used for generating the initial light signal emitted at the first preset angle according to the laser signal.

7. The microscopic imaging detection system of claim 6, wherein the oblique incidence unit comprises:

the aspheric lens is coupled with the LED light source;

a first attenuation sheet coupled to the aspheric lens;

a second attenuation sheet coupled with the first attenuation sheet;

the first optical filter is coupled with the second attenuation sheet; the aspheric lens, the first attenuation sheet, the second attenuation sheet and the first optical filter are all used for modulating the laser signal;

the dichroic mirror is coupled and connected with the first optical filter at a second preset angle, and the second preset angle is smaller than 90 degrees; the dichroic mirror is used for reflecting the modulated laser signal to obtain a reflection signal;

an achromatic lens; the achromatic lens and the dichroic mirror are in coupling connection at a third preset angle, and the third preset angle is smaller than 90 degrees; the achromatic lens is used for processing the reflection signal to obtain the initial optical signal.

8. The microscopic imaging detection system of claim 1, wherein the imaging module comprises:

a tri-cemented lens coupled with the carrier;

the second optical filter is coupled with the three cemented lenses; the tri-cemented lens and the second optical filter are used for modulating the fluorescent light signal;

and the imaging lens is coupled with the second optical filter and is used for focusing the fluorescent light signal to obtain the target light signal.

9. The microscopic imaging detection system according to any one of claims 1 to 8, further comprising:

a housing for sealing the imaging detection device;

wherein, the material of casing is light tight material.

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