CN110487767B - Portable up-conversion fluorescence detector - Google Patents
Portable up-conversion fluorescence detector Download PDFInfo
- Publication number
- CN110487767B CN110487767B CN201910829513.6A CN201910829513A CN110487767B CN 110487767 B CN110487767 B CN 110487767B CN 201910829513 A CN201910829513 A CN 201910829513A CN 110487767 B CN110487767 B CN 110487767B
- Authority
- CN
- China
- Prior art keywords
- sample
- light
- detector
- module
- bin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Abstract
The invention relates to a portable up-conversion fluorescence detector, which belongs to the technical field of image recognition and comprises an instrument main body and a replaceable sample module, wherein a main control chip is arranged in the instrument main body, one end of the main control chip is connected with a light source module, the other end of the main control chip is connected with a detector, and the main control chip is also connected with a battery, a wireless module and a USB charging module; the replaceable sample module is connected with the instrument main body through a buckle, a sample bin is arranged in the replaceable sample module, the light source module is detachably connected with one end of the sample bin through an optical fiber connector, and the other end of the sample bin is detachably connected with the detector through a lens element; the replaceable sample module is provided with different sample bins and light path systems according to different sample types; the detector comprises a convex lens and a photosensitive element, and is used for collecting fluorescence signals excited by the sample and imaging; the main control chip is used for receiving the sample fluorescence signal imaging information collected by the photosensitive element, carrying out image recognition and processing and calculating the sample concentration.
Description
Technical Field
The invention belongs to the technical field of image recognition, and relates to a portable up-conversion fluorescence detector.
Background
The upconversion luminescence technology is a nonlinear optical process, an upconversion material excites electrons to transit to high energy level by absorbing a plurality of photons, and after relaxation transition to each vibration energy level of a ground state, luminescence is carried out, and the luminescence frequency is subjected to upconversion relative to incident light. Because near-infrared wavelength excitation is used, compared with a conventional fluorescent material, the background substance does not generate an up-conversion fluorescent signal, the signal-to-noise ratio is greatly improved, and the detection sensitivity is improved.
The up-conversion material has the characteristics of high chemical stability, light stability, narrow-band emission, strong penetration of exciting light, no damage and the like, and has good application prospect in the fields of fluorescent probes, biological imaging, immunodetection, chemical detection and the like. At present, most of instruments for up-conversion luminescence detection are a desk type fluorescence analyzer, a portable immunoassay analyzer and the like, light sources of the desk type fluorescence analyzer for laboratory use are mainly distributed in an ultraviolet visible wave band, the requirements on infrared excited up-conversion luminescence material light sources cannot be well met, the portable immunoassay analyzer is only applied to the medical field and the biological field, and the detection requirements in other subject fields such as environmental science and food science cannot be met.
Secondly, the upconversion material mainly comprises upconversion nanoparticles dispersed in various matrixes, and is various in sample forms aiming at different subject fields, wherein the sample forms comprise nanoparticle solutions, fluorescent test paper, fluorescent chips and the like, and the traditional laboratory means cannot meet the requirements of high flux, portability and instantaneity detection in the practical application of each subject field.
Therefore, aiming at the requirements of high flux, portability, instantaneity detection and the like which are possibly met in different subject fields, various types of sample pools are required to be coupled on one instrument, various sample measurement is realized by methods such as replacing the sample pools and the like, the portable instrument is suitable for scenes such as field measurement, family detection and the like, and high-flux sample information reading is realized through a certain technology.
Disclosure of Invention
In view of the above, the present invention provides a portable upconversion fluorescence detector for detecting different types of samples in different types and replaceable sample chamber modules by using an image recognition technology, wherein the samples are excited by a near infrared light source, a light path system collects signals to form specific light spots on the detector, and wavelength and intensity distribution information of the light spots in different areas are extracted by processing, so as to achieve the purpose of single-channel and multi-channel automatic detection of multiple types of samples.
In order to achieve the purpose, the invention provides the following technical scheme:
a portable up-conversion fluorescence detector comprises an instrument main body and a replaceable sample module, wherein a main control chip is arranged in the instrument main body, one end of the main control chip is connected with a light source module used for providing near-infrared laser for exciting up-conversion fluorescence, the other end of the main control chip is connected with a detector, and the main control chip is also connected with a battery, a wireless module and a USB charging module;
the replaceable sample module is connected with the instrument main body through a buckle, a sample bin is arranged in the replaceable sample module, the sample is inserted into the sample bin through an opening on one side of the sample bin, a light source module in the instrument main body is detachably connected with one end of the sample bin through an optical fiber connector, and the other end of the sample bin is detachably connected with the detector through a lens element;
the replaceable sample modules are various, and are different in that different sample bins and light path systems in the sample bins are arranged according to different sample types, wherein the sample types comprise fluorescence chromatography test paper, a PCR (polymerase chain reaction) tube, a cuvette and an array chip;
the optical path system comprises a transmission type sample bin optical path system and a reflection type sample bin optical path system, the transmission type sample bin optical path system is used for adapting to a PCR tube and a cuvette sample, and the reflection type sample bin optical path system is used for adapting to a fluorescence chromatography test paper and an array chip sample;
the transmission type sample bin optical path system comprises an optical fiber splitter connected with an optical fiber connector of a light source module, and further comprises an optical filter, a first lens group and a reflector, wherein the light source is split into a series of equal-intensity light sources through the optical fiber splitter, incident light is filtered out through the optical filter after passing through a sample, then a light spot signal is reduced through the first lens group, and then the light spot signal is reflected into a detector through the reflector;
the reflection type sample bin optical path system comprises a second lens group, a prism arranged on a sample at an angle of 45 degrees, an optical filter, a third lens group and a reflector, wherein a light source amplifies light spots through the second lens group, irradiates the prism, is refracted to the sample through the prism to excite fluorescence, the fluorescence is reflected to the optical filter through the prism to filter out incident light, a light spot signal is reduced through the third lens group, and the light spot signal is reflected to a detector through the reflector;
the detector comprises a convex lens and a photosensitive element, and is used for collecting fluorescence signals excited by the sample and imaging;
the main control chip is used for receiving the sample fluorescence signal imaging information collected by the photosensitive element, carrying out image recognition and processing and calculating the sample concentration.
Further, the sample chamber is in a multi-channel form and comprises a plurality of sample grooves for placing samples, the positions of the sample grooves are fixed relative to the sample chamber, and then the imaging positions of fluorescent signals emitted by the samples placed in the sample grooves on the photosensitive element are fixed, namely, a specific sample groove corresponds to a specific channel at a specific image position corresponding to the sample; marking sample bin type serial numbers on the sample bins, and identifying the sample serial numbers of light spots at different positions by setting the sample bin serial numbers to automatically match a system program when different sample bins are connected;
no matter how many samples are in the sample bin, the serial numbers of the samples corresponding to the samples are identified by an image identification method, the concentration of the samples is calculated, and the system divides channels for different areas of the photosensitive element and displays the concentration result in the corresponding channel.
Further, the photosensitive element in the detector is a CCD or CMOS photosensitive element, images formed on the CCD or CMOS photosensitive element are in two different types of optical path systems, the images of the sample are all light spots or strips having a certain RGB value, and the range of the light spots is identified by an image processing technique:
firstly, binarization processing is carried out on image information, a threshold value is set through an OTSU method to identify a light spot and a background, so that light spot area information is obtained, namely 256 brightness levels from 0 to 255 of gray values of pixel points on an image are set to be 0 or 255 through threshold value selection, even if the difference between a target and the background is the maximum in an OTSU algorithm, assuming that the segmentation threshold value of the foreground and the background is t, the ratio w of the foreground points is w0Mean value u0Background dot ratio w1Mean value u1Mean value of picture u ═ w0*u0+w1*u1Establishing an objective function g (t) w0*(u0-u)2+w1*(u1-u)2Solving the maximum value of g (t), wherein the corresponding value of t is the optimal threshold value;
the area of the gray level 255 obtained after the image is subjected to binarization processing is a corresponding area of the sample, different light spots in the image are subjected to frame selection through an image recognition technology, and RGB information of all pixel points in the area is extracted, wherein the RGB information corresponds to information such as current and voltage of a TFT circuit in a CCD or CMOS photosensitive element, and corresponds to light intensity and wave number information:
F(x,y,z)＝x(R)+y(G)+z(B)
calculating RGB information of the light spot to obtain light field distribution of the light spot, and obtaining intensity and wave number distribution information of the light spot;
the wave number and the intensity information of the light spots correspond to the fluorescence signals of the sample, the fluorescence signals of the standard samples with a series of concentrations are detected, the sample to be detected can be detected after the marked line is drawn, and the concentration of the sample to be detected can be calculated through the marked line.
Further, the first lens group and the third lens group each include a convex lens and a concave lens arranged from the incident side to the exit side for reducing the light spot, and the second lens group includes a concave lens and a convex lens arranged from the incident side to the exit side for enlarging the light spot.
Further, an operation area and a display area are further arranged on the instrument main body, the operation area is used for carrying out power supply control on the detector, and the display area is used for displaying the type and concentration of the sample.
Further, the wireless module is used for communicating with the PC client or the mobile terminal, sharing data to the PC client or the mobile terminal, and controlling the detector through the PC client or the mobile terminal.
The invention has the beneficial effects that: compared with the prior art, the invention can change the sample bin and the light path system according to different sample types and adapt the detection method through the replaceable sample module.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Drawings
For the purposes of promoting a better understanding of the objects, aspects and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic external view of a portable up-conversion fluorescence detector according to the present invention; (ii) a
FIG. 2 is a diagram of the internal connection of the portable up-conversion fluorescence detector according to the present invention;
FIG. 3 is a schematic view of the instrument body and sample module according to the present invention;
FIG. 4 is a schematic diagram of a transmission type sample chamber optical path system;
FIG. 5 is a schematic diagram of a reflective sample chamber optical path system;
fig. 6 is a schematic diagram of an image processing process.
Reference numerals: the device comprises a sample module 1, an instrument main body 2, a display area 21, an operation area 3, a main control chip 4, a light source module 5, a detector 6, a sample bin 7, a buckle 8, an optical fiber 9, a battery 10, a wireless module 11 and a USB charging module 12.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention in a schematic way, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in the drawings in which there is no intention to limit the invention thereto; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "front", "rear", etc., based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not an indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes, and are not to be construed as limiting the present invention, and the specific meaning of the terms may be understood by those skilled in the art according to specific situations.
Aiming at the problems of portability and sample diversity in the field of up-conversion fluorescence detection at present, the invention provides a portable up-conversion fluorescence detector, which detects different types of samples in different types and replaceable sample bin modules through an image recognition technology, the samples are excited by a near infrared light source, a light path system collects signals, specific light spots are formed on a detector, and the wavelength and intensity distribution information of the light spots in different areas is processed and extracted, so that the purpose of single-channel and multi-channel automatic detection of multiple types of samples is achieved.
As shown in fig. 1-2, a portable up-conversion fluorescence detector includes an instrument main body 2 and a replaceable sample module 1, wherein a main control chip 4 is disposed in the instrument main body 2, one end of the main control chip 4 is connected to a light source module 5 for providing near-infrared laser for exciting up-conversion fluorescence, and multiple wavelengths such as 805nm and 980nm are provided; the other end of the main control chip 4 is connected with a detector 6, and the main control chip 4 is also connected with a battery 10, a wireless module 11 and a USB charging module 12;
the replaceable sample module 1 is connected with the instrument main body 2 through a buckle 8, the buckle 8 is designed on the instrument main body 2 and two sides of the sample module 1, and the replaceable sample module is fixed through the buckle 8; moreover, as shown in fig. 3, a groove and a protrusion engagement positioning structure are designed on the connection surface of the sample module 1 and the instrument main body 2 to ensure the accuracy of instrument connection, a sample chamber 7 is arranged in the replaceable sample module 1, the sample is inserted into the sample chamber 7 through an opening at one side of the sample chamber 7, the light source module 5 in the instrument main body 2 is detachably connected with one end of the sample chamber 7 through an optical fiber 9 joint, and the other end of the sample chamber 7 is detachably connected with the detector 6 through a lens element; the sample bin 7 with various adaptive types is provided, detection can be performed on various sample types such as fluorescence chromatography test paper, a PCR tube, a cuvette, an array chip and the like, a single-channel and multi-channel optical fiber 9 light splitting system and a detection system can be adapted according to the sample types, a single sample can be measured, and multiple integrated samples can also be measured;
the replaceable sample module 1 is various, and is characterized in that different sample bins 7 and optical path systems in the sample bins 7 are arranged according to different sample types, wherein the sample types comprise fluorescence chromatography test paper, a PCR tube, a cuvette and an array chip;
the optical path system comprises a transmission type sample bin 7 optical path system and a reflection type sample bin 7 optical path system, wherein the transmission type sample bin 7 optical path system is used for adapting to a PCR tube and a cuvette sample, and the reflection type sample bin 7 optical path system is used for adapting to a fluorescence chromatography test paper and an array chip sample;
as shown in fig. 4, the light path system of the transmissive sample chamber 7 includes an optical fiber 9 splitter connected to an optical fiber 9 connector of the light source module 5, and further includes an optical filter, a first lens group, and a reflector, where the light source is split into a series of equal-intensity light sources by the optical fiber 9 splitter, passes through the sample, and then passes through the optical filter to filter out incident light, and then passes through the first lens group to reduce the light spot signal, and then is reflected by the reflector to the detector 6;
as shown in fig. 5, the optical path system of the reflective sample chamber 7 includes a second lens group, a prism disposed on the sample at an angle of 45 degrees, and further includes an optical filter, a third lens group and a reflector, the light source amplifies the light spot through the second lens group, irradiates on the prism, refracts on the sample through the prism to excite fluorescence, the fluorescence is reflected to the optical filter through the prism to filter out incident light, then reduces the light spot signal through the third lens group, and reflects the light spot signal through the reflector to the detector 6;
the detector 6 comprises a convex lens and a photosensitive element, and is used for collecting fluorescence signals excited by the sample and imaging;
and the main control chip 4 is used for receiving the sample fluorescence signal imaging information collected by the photosensitive element, identifying and processing the image and calculating the sample concentration.
The sample chamber 7 is in a multi-channel form and comprises a plurality of sample grooves for placing samples, the positions of the sample grooves relative to the sample chamber 7 are fixed, and then the imaging positions of fluorescent signals emitted by the samples placed in the sample grooves on the photosensitive element are fixed, namely, a specific sample groove corresponds to a specific channel at a specific image position corresponding to the sample imaging; the sample bin 7 is marked with a sample bin 7 type serial number, and when different sample bins 7 are connected, the serial numbers of the samples of light spots at different positions are identified by setting the serial number of the sample bin 7 to automatically match a system program;
no matter how many samples are in the sample bin 7, the serial numbers of the corresponding samples are identified by an image identification method, the concentration of the samples is calculated, and the system divides channels for different areas of the photosensitive element and displays the concentration result in the corresponding channel.
The light sensing element in the detector 6 is a CCD or CMOS light sensing element, images formed on the CCD or CMOS light sensing element are in two different types of light path systems, the images of the sample are light spots or strips with certain RGB values, and the range of the light spots is identified through an image processing technology:
as shown in fig. 6, firstly, the image information is binarized, and the threshold is set by the OTSU method to identify the light spot and the background, so as to obtain the light spot area information, that is, 256 brightness levels from 0 to 255 of the gray value of the pixel point on the image are set to be 0 or 255 through threshold selection, even if the inter-class variance between the target and the background is maximum in the OTSU algorithm, assuming that the segmentation threshold of the foreground and the background is t, the ratio w of the foreground point to the background is w0Mean value u0Background dot ratio w1Mean value u1Mean value of picture u ═ w0*u0+w1*u1Establishing an objective function g (t) w0*(u0-u)2+w1*(u1-u)2Solving the maximum value of g (t), wherein the corresponding value of t is the optimal threshold value;
the area of the gray level 255 obtained after the image is subjected to binarization processing is a corresponding area of the sample, different light spots in the image are subjected to frame selection through an image recognition technology, and RGB information of all pixel points in the area is extracted, wherein the RGB information corresponds to information such as current and voltage of a TFT circuit in a CCD or CMOS photosensitive element, and corresponds to light intensity and wave number information:
F(x,y,z)＝x(R)+y(G)+z(B)
calculating RGB information of the light spot to obtain light field distribution of the light spot, and obtaining intensity and wave number distribution information of the light spot;
the wave number and the intensity information of the light spots correspond to the fluorescence signals of the sample, the fluorescence signals of the standard samples with a series of concentrations are detected, the sample to be detected can be detected after the marked line is drawn, and the concentration of the sample to be detected can be calculated through the marked line.
The first lens group and the third lens group respectively comprise a convex lens and a concave lens which are arranged from an incidence side to an emergence side and are used for reducing light spots, and the second lens group comprises a concave lens and a convex lens which are arranged from the incidence side to the emergence side and are used for amplifying the light spots.
The instrument main body 2 is further provided with an operation area 3 and a display area 21, the operation area 3 is used for controlling a power supply of the detector, and the display area 21 is used for displaying the type and concentration of the sample.
The wireless module 11 is used for communicating with a PC client or a mobile terminal, sharing data to the PC client or the mobile terminal, and controlling the detector through the PC client or the mobile terminal.
And (3) detection process:
1. selecting a corresponding sample bin type according to a sample to be detected;
2. fixing the corresponding sample bin on the instrument, opening the instrument, and setting the serial number of the sample bin;
3. placing standard samples with different concentrations in different channels of a sample bin in sequence, and detecting fluorescence intensity;
4. drawing a marking line according to the concentration and the fluorescence intensity of the standard sample, and storing the marking line in an instrument;
5. and setting the marked line as the marked line, detecting the fluorescence intensity of the sample to be detected, and automatically giving the concentration by an instrument.
Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.
Claims (4)
1. A portable up-conversion fluorescence detector is characterized in that: the device comprises an instrument main body and a replaceable sample module, wherein a main control chip is arranged in the instrument main body, one end of the main control chip is connected with a light source module used for providing near-infrared laser for exciting up-conversion fluorescence, the other end of the main control chip is connected with a detector, and the main control chip is also connected with a battery, a wireless module and a USB charging module;
the replaceable sample module is connected with the instrument main body through a buckle, a sample bin is arranged in the replaceable sample module, a sample is inserted into the sample bin through an opening on one side of the sample bin, a light source module in the instrument main body is detachably connected with one end of the sample bin through an optical fiber connector, and the other end of the sample bin is detachably connected with the detector through a lens element;
the sample chamber is in a multi-channel form and comprises a plurality of sample grooves for placing samples, and the positions of the sample grooves relative to the sample chamber are fixed, so that the imaging positions of fluorescent signals emitted by the samples placed in the sample grooves on the photosensitive element are fixed, namely, a specific sample groove corresponds to a specific channel at a specific image position corresponding to the sample imaging; marking sample bin type serial numbers on the sample bins, and identifying the sample serial numbers of light spots at different positions by setting the sample bin serial numbers to automatically match a system program when different sample bins are connected;
no matter how many samples are in the sample bin, the serial numbers of the corresponding samples are identified by an image identification method, the concentration of the samples is calculated, and the system divides channels for different areas of the photosensitive element and displays the concentration result in the corresponding channel;
the replaceable sample modules are various, and are different in that different sample bins and light path systems in the sample bins are arranged according to different sample types, wherein the sample types comprise fluorescence chromatography test paper, a PCR (polymerase chain reaction) tube, a cuvette and an array chip;
the optical path system comprises a transmission type sample bin optical path system and a reflection type sample bin optical path system, the transmission type sample bin optical path system is used for adapting to a PCR tube and a cuvette sample, and the reflection type sample bin optical path system is used for adapting to a fluorescence chromatography test paper and an array chip sample;
the transmission type sample bin optical path system comprises an optical fiber splitter connected with an optical fiber connector of a light source module, and further comprises an optical filter, a first lens group and a reflector, wherein the light source is split into a series of equal-intensity light sources through the optical fiber splitter, incident light is filtered out through the optical filter after passing through a sample, then a light spot signal is reduced through the first lens group, and then the light spot signal is reflected into a detector through the reflector;
the reflection type sample bin optical path system comprises a second lens group, a prism arranged on a sample at an angle of 45 degrees, an optical filter, a third lens group and a reflector, wherein a light source amplifies light spots through the second lens group, irradiates the prism, is refracted to the sample through the prism to excite fluorescence, the fluorescence is reflected to the optical filter through the prism to filter out incident light, a light spot signal is reduced through the third lens group, and the light spot signal is reflected to a detector through the reflector;
the detector comprises a convex lens and a photosensitive element, and is used for collecting fluorescence signals excited by the sample and imaging;
the main control chip is used for receiving the sample fluorescence signal imaging information collected by the photosensitive element, carrying out image recognition and processing and calculating the sample concentration;
the photosensitive element in the detector is a CCD or CMOS photosensitive element, images formed on the CCD or CMOS photosensitive element are in two different types of light path systems, the images of the sample are light spots or strips with certain RGB values, and the range of the light spots is identified through an image processing technology:
firstly, binarization processing is carried out on image information, a threshold value is set through an OTSU method to identify a light spot and a background, so that light spot area information is obtained, namely 256 brightness levels from 0 to 255 of gray values of pixel points on an image are set to be 0 or 255 through threshold value selection, even if the difference between a target and the background is the maximum in an OTSU algorithm, assuming that the segmentation threshold value of the foreground and the background is t, the ratio w of the foreground points is w0Mean value u0Back of bodyRatio of attraction w1Mean value u1Mean value of picture u ═ w0*u0+w1*u1Establishing an objective function g (t) w0*(u0-u)2+w1*(u1-u)2Solving the maximum value of g (t), wherein the corresponding value of t is the optimal threshold value;
the area of the gray level 255 obtained after the image is subjected to binarization processing is a corresponding area of the sample, different light spots in the image are subjected to frame selection through an image recognition technology, and RGB information of all pixel points in the area is extracted, wherein the RGB information corresponds to information such as current and voltage of a TFT circuit in a CCD or CMOS photosensitive element, and corresponds to light intensity and wave number information:
F(x,y,z)＝x(R)+y(G)+z(B)
calculating RGB information of the light spot to obtain light field distribution of the light spot, and obtaining intensity and wave number distribution information of the light spot;
the wave number and the intensity information of the light spots correspond to the fluorescence signals of the sample, the fluorescence signals of the standard samples with a series of concentrations are detected, the sample to be detected can be detected after the marked line is drawn, and the concentration of the sample to be detected can be calculated through the marked line.
2. The portable up-conversion fluorescence detector of claim 1, wherein: the first lens group and the third lens group respectively comprise a convex lens and a concave lens which are arranged from an incidence side to an emergence side and are used for reducing light spots, and the second lens group comprises a concave lens and a convex lens which are arranged from the incidence side to the emergence side and are used for amplifying the light spots.
3. The portable up-conversion fluorescence detector of claim 1, wherein: the instrument main body is further provided with an operation area and a display area, the operation area is used for controlling the power supply of the detector, and the display area is used for displaying the type and concentration of the sample.
4. The portable up-conversion fluorescence detector of claim 1, wherein: the wireless module is used for communicating with the PC client or the mobile terminal, sharing data to the PC client or the mobile terminal, and controlling the detector through the PC client or the mobile terminal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910829513.6A CN110487767B (en) | 2019-09-03 | 2019-09-03 | Portable up-conversion fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910829513.6A CN110487767B (en) | 2019-09-03 | 2019-09-03 | Portable up-conversion fluorescence detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110487767A CN110487767A (en) | 2019-11-22 |
| CN110487767B true CN110487767B (en) | 2022-03-25 |
Family
ID=68556033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910829513.6A Active CN110487767B (en) | 2019-09-03 | 2019-09-03 | Portable up-conversion fluorescence detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110487767B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111398240B (en) * | 2020-05-21 | 2022-11-08 | 中国科学院重庆绿色智能技术研究院 | Fluorescence detection adapter for up-conversion of superlens |
| CN113092428B (en) * | 2021-04-02 | 2023-07-25 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
| CN114441497B (en) * | 2022-02-24 | 2023-10-20 | 重庆尚立仪器设备有限公司 | Heavy metal ion detection method based on quantum dot fluorescent probe array |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901190A (en) * | 2014-04-17 | 2014-07-02 | 武汉纽康度生物科技有限公司 | IF (immunofluorescence) quantitative analysis meter |
| CN107884571A (en) * | 2016-09-30 | 2018-04-06 | 张磊 | Portable fluorescence immunity analysis instrument and Portable fluorescence analysis method |
| CN107941771A (en) * | 2017-12-13 | 2018-04-20 | 西安交通大学 | A kind of modularization up-conversion fluorescence chromatographic test paper excitation apparatus |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN207908517U (en) * | 2018-03-27 | 2018-09-25 | 中国人民解放军第三〇二医院 | Portable upper forwarding light immunity analysis instrument |
| CN109696404A (en) * | 2017-10-20 | 2019-04-30 | 西南大学 | Portable multi-channel detector |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120280144A1 (en) * | 2011-03-29 | 2012-11-08 | Guilfoyle Richard A | Optical system enabling low power excitation and high sensitivity detection of near infrared to visible upconversion phoshors |
-
2019
- 2019-09-03 CN CN201910829513.6A patent/CN110487767B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901190A (en) * | 2014-04-17 | 2014-07-02 | 武汉纽康度生物科技有限公司 | IF (immunofluorescence) quantitative analysis meter |
| CN107884571A (en) * | 2016-09-30 | 2018-04-06 | 张磊 | Portable fluorescence immunity analysis instrument and Portable fluorescence analysis method |
| CN109696404A (en) * | 2017-10-20 | 2019-04-30 | 西南大学 | Portable multi-channel detector |
| CN107941771A (en) * | 2017-12-13 | 2018-04-20 | 西安交通大学 | A kind of modularization up-conversion fluorescence chromatographic test paper excitation apparatus |
| CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
| CN207908517U (en) * | 2018-03-27 | 2018-09-25 | 中国人民解放军第三〇二医院 | Portable upper forwarding light immunity analysis instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110487767A (en) | 2019-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110487767B (en) | Portable up-conversion fluorescence detector | |
| CN105493258B (en) | For the defects detection of sample and the system and method for photoluminescence measurement | |
| US7186990B2 (en) | Method and apparatus for detecting and imaging the presence of biological materials | |
| US6999173B2 (en) | Method and apparatus for ratio fluorometry | |
| TWI263072B (en) | Method and system for reading microarrays | |
| CN201653906U (en) | Biological immunochromatography chip detector | |
| EP1036371A1 (en) | High throughput optical scanner | |
| JP6501714B2 (en) | Optical survey device | |
| US20070177149A1 (en) | Instrumentation and method for optical measurement of samples | |
| US11112362B2 (en) | Portable in-vitro diagnostic detector and apparatus | |
| CN101688834A (en) | Microelectronic sensor device with light source and light detector | |
| CN102590169A (en) | Fluorescent chromatography detector | |
| CN105637349A (en) | Series absorbance glass and interference filters | |
| US20100141938A1 (en) | Method and apparatus for detection of analytes | |
| US20210215605A1 (en) | Sensor for lifetime plus spectral characterization | |
| JP4887475B2 (en) | System and method for using multiple detection channels to eliminate autofluorescence | |
| CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
| CN113189065B (en) | Optical detection method | |
| EP3921627B1 (en) | Method of analyzing samples, analyzing device and computer program | |
| KR20160014340A (en) | System for analyzing fluorescence lifetime and analysis method of fluorescence lifetime using the same | |
| CN213148955U (en) | Fluorescence immunoassay system | |
| CN217820406U (en) | Reagent kit | |
| CN217739001U (en) | Microscopic imaging detection system | |
| TWI820406B (en) | Detection equipment for detecting biological particles and detection method of detection equipment | |
| CN103472040B (en) | Fluorescence detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |