CN110487767A - A kind of portable up-conversion fluorescence detector - Google Patents
A kind of portable up-conversion fluorescence detector Download PDFInfo
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- CN110487767A CN110487767A CN201910829513.6A CN201910829513A CN110487767A CN 110487767 A CN110487767 A CN 110487767A CN 201910829513 A CN201910829513 A CN 201910829513A CN 110487767 A CN110487767 A CN 110487767A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The present invention relates to a kind of portable up-conversion fluorescence detectors, belong to image identification technical field, including apparatus subject and changeable type sample block, it is equipped with main control chip one end in apparatus subject and is connected with light source module, the other end is connected with detector, and main control chip is also connected with battery, wireless module and USB charging module;Changeable type sample block is connect by buckle with apparatus subject, inside sets sample bin, light source module is detachably connected by one end of fibre-optical splice and sample bin, and the sample bin other end is detachably connected by lens element and detector;Different sample bin and light path system are arranged according to different sample types for changeable type sample block;It include convex lens and photosensitive element in the detector, for collecting fluorescence signal and the imaging of sample excitation;The main control chip is used to receive the sample fluorescence signal image-forming information of photosensitive element acquisition and carries out image recognition and processing, calculates sample concentration.
Description
Technical field
The invention belongs to image identification technical fields, are related to a kind of portable up-conversion fluorescence detector.
Background technique
Up-converting phosphor technology is a kind of nonlinear optical process, and up-conversion is by absorbing multiple photonexcited electrons
Transit to high level, after relaxation transition to each vibration level of ground state shine, luminous frequency relative to incident light occur on convert.
Due to using near-infrared wavelength to excite, relative to conventional fluorescent material, background material does not generate up-conversion fluorescence signal, mentions significantly
High signal-to-noise ratio, improves the sensitivity of detection.
Up-conversion chemical stability with higher, photostability, narrow emission, excitation light peneration are strong, lossless
The characteristics such as wound, have good application prospect in fields such as fluorescence probe, bio-imaging, immune detection, chemical detections.It is directed at present
The instrument majority of up-conversion luminescence detection is desk-top fluorescence analyser, portable immunity analysis instrument etc., the desk-top fluorescence of use for laboratory point
Its light source of analyzer is mainly distributed on UV, visible light wave band, can not expire very well to the up-conversion luminescent material light source requirements of infrared excitation
Foot, portable immunity analysis instrument application are only limitted to medicine, biological field, and the Other subjects such as environmental science, Food Science are led
The detection demand in domain is also unable to satisfy.
Secondly, up-conversion is mainly made of the upper conversion nano particle being dispersed in various matrix, for not classmate
Section field, sample form multiplicity, including nanoparticles solution, fluorescent test paper, Fluorescence chip etc., traditional laboratory techniques can not
Meets the needs of high throughput in each ambit practical application, portability, instantaneity detection.
The demands such as high throughput, portability, the instantaneity detection being therefore likely encountered for different ambits, needing will be more
The sample cell of seed type is coupled on an instrument, realizes various samples measurement by the methods of replacement sample cell, portability
Instrument adapts to field measurement, family's detection etc. scene, realizes that high-throughput sample message is read by certain technology.
Summary of the invention
In view of this, more it is an object of the invention to be directed to the portability of current up-conversion fluorescence detection field and sample
Sample problem provides a kind of portable up-conversion fluorescence detector, by image recognition technology to different type, replaceable sample
Different types of sample is detected in the module of product storehouse, and sample passes through near infrared light source excitation, and light path system collecting signal is being visited
The hot spot for forming specificity on device is surveyed, the wherein wavelength of the hot spot of different zones, strength distributing information are extracted by processing, reached
The purpose of the single channel of multiple types sample, multicenter auto-inspected.
In order to achieve the above objectives, the invention provides the following technical scheme:
A kind of portable up-conversion fluorescence detector, including apparatus subject and changeable type sample block, the instrument master
It is equipped with main control chip in vivo, described main control chip one end is connected with the light of the near-infrared laser for providing excitation up-conversion fluorescence
Source module, the other end are connected with detector, and the main control chip is also connected with battery, wireless module and USB charging module;
The changeable type sample block is connect by buckle with apparatus subject, is equipped in the changeable type sample block
Sample bin, the sample are inserted into sample bin by the opening of sample bin side, and the light source module in the apparatus subject passes through light
One end of fine connector and sample bin is detachably connected, and the other end of the sample bin is detachably connected by lens element with detector
It connects;
There are many changeable type sample blocks, and difference is that different sample bins is arranged according to different sample types
And the light path system in sample bin, the sample type include fluorescent chromatographic test paper, PCR pipe, cuvette and array chip;
The light path system includes transmission-type sample bin light path system and reflection-type sample bin light path system, the transmission-type
Sample bin light path system is for being adapted to PCR pipe and cuvette sample, and the reflection-type sample bin light path system is for being adapted to fluorescence
Chromatographic test paper and array chip sample;
The transmission-type sample bin light path system includes the fiber splitter connecting with the fibre-optical splice of light source module, is also wrapped
Optical filter, the first lens group and reflecting mirror are included, light source splits into the intense light sources such as a series of by fiber splitter, after sample
Incident light is filtered off through optical filter, is then reduced spot signal by the first lens group, then detection is reflected by reflecting mirror
In device;
The prism that the reflection-type sample bin light path system includes the second lens group, is placed in 45 degree of angles on sample, also
Including optical filter, the third lens group and reflecting mirror, light source amplifies hot spot by the second lens group, is radiated on prism,
By exciting fluorescence in refraction by prism to sample, fluorescence is by filtering off incident light on prismatic reflection to optical filter, using third
Lens group reduces spot signal, is reflected into detector by reflecting mirror;
It include convex lens and photosensitive element in the detector, for collecting fluorescence signal and the imaging of sample excitation;
The main control chip is used to receive the sample fluorescence signal image-forming information of photosensitive element acquisition and carries out image recognition
And processing, calculate sample concentration.
Further, the sample bin is multichannel form, and including multiple sample cells for placing sample, the sample cell is opposite
It is fixed in sample bin location, then the fluorescence signal for being placed on the electromagnetic radiation in sample cell is consolidated imaging position on photosensitive element
Fixed, i.e., specific sample cell counter sample is imaged on image specific position and corresponds to special modality always;In the sample bin subscript
Show sample bin type serial number, when connecting different sample bins, by setting sample bin serial number automatic patching system program, to know
The sample serial number of other different location hot spot;
No matter sample size is how many in sample bin, its counter sample serial number is identified by the method for image recognition, is calculated
Its concentration, system divide channel by the different zones to photosensitive element, concentration results are shown in corresponding channel.
Further, the photosensitive element in the detector is CCD or CMOS photosensitive element, on CCD or CMOS photosensitive element
For imaging in two distinct types of light path system, the picture of sample is hot spot or band with certain rgb value, is led to
Cross the range of image processing techniques identification hot spot:
Binary conversion treatment is carried out to image information first, hot spot and background are identified by OTSU method given threshold, thus
To spot area information, i.e., 256 brightness degrees of the gray value 0 to 255 of the pixel on image are set by threshold value selection
It is set to 0 or 255, even if the inter-class variance of OTSU algorithm target and two class of background is maximum, it is assumed that the segmentation threshold of prospect and background
For t, foreground point accounting w0, mean value u0, background dot accounting w1, mean value u1, image mean value u=w0*u0+w1*u1, establish objective function g
(t)=w0*(u0-u)2+w1*(u1-u)2, g (t) maximum value is solved, corresponding to t value at this time is optimal threshold;
The region that the gray scale 255 that binary conversion treatment obtains later is carried out to image is sample corresponding region, passes through image
Identification technology carries out frame choosing to the different hot spots in image, and extracts the RGB information of all pixels point in region, RGB letter
The information such as electric current, voltage of TFT circuit in corresponding CCD or CMOS photosensitive element are ceased, the intensity of corresponding light, wavenumber information:
F (x, y, z)=x (R)+y (G)+z (B)
The RGB information for calculating hot spot obtains its optical field distribution, obtains intensity, the wave number distributed intelligence of hot spot;
The wave number of hot spot, strength information have corresponded to the fluorescence signal of sample, pass through a series of the glimmering of the standard items to concentration
Optical signal is detected, and after drawing graticule, can be detected to sample to be tested, and calculate its concentration by graticule.
Further, first lens group and the third lens group include a convex lens being arranged by incidence side to outgoing side
Mirror and a concavees lens, for reducing hot spot, second lens group includes that be arranged by incidence side to outgoing side one is recessed
Mirror and a convex lens, for amplifying hot spot.
Further, operating area and display area are additionally provided on the apparatus subject, the operating area is used for detection
Instrument carries out power supply control, and the display area is for showing sample classification and concentration.
Further, the wireless module be used for pc client or communication of mobile terminal, by data sharing to pc client
Or mobile terminal, and detector is controlled by pc client or mobile terminal.
The beneficial effects of the present invention are: compared with prior art, the present invention, can be according to sample by changeable type sample block
Category type is different, replaces sample bin and light path system, is adapted to detection method, portability of the present invention is strong, and structure is simple, operation side
Just.
Other advantages, target and feature of the invention will be illustrated in the following description to a certain extent, and
And to a certain extent, based on will be apparent to those skilled in the art to investigating hereafter, Huo Zheke
To be instructed from the practice of the present invention.Target of the invention and other advantages can be realized by following specification and
It obtains.
Detailed description of the invention
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made below in conjunction with attached drawing excellent
The detailed description of choosing, in which:
Fig. 1 is portable up-conversion fluorescence detector outside schematic diagram of the present invention;;
Fig. 2 is connection figure inside portable up-conversion fluorescence detector of the present invention;
Fig. 3 is apparatus subject of the present invention, sample block connection schematic diagram;
Fig. 4 is transmission-type sample bin light path system schematic diagram;
Fig. 5 is reflection-type sample bin light path system schematic diagram;
Fig. 6 is image processing process schematic diagram.
Appended drawing reference: sample block 1, apparatus subject 2, display area 21, operating area 3, main control chip 4, light source module
5, detector 6, sample bin 7, buckle 8, optical fiber 9, battery 10, wireless module 11, USB charging module 12.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.It should be noted that diagram provided in following embodiment is only to show
Meaning mode illustrates basic conception of the invention, and in the absence of conflict, the feature in following embodiment and embodiment can phase
Mutually combination.
Wherein, the drawings are for illustrative purposes only and are merely schematic diagrams, rather than pictorial diagram, should not be understood as to this
The limitation of invention;Embodiment in order to better illustrate the present invention, the certain components of attached drawing have omission, zoom in or out, not
Represent the size of actual product;It will be understood by those skilled in the art that certain known features and its explanation may be omitted and be in attached drawing
It is understood that.
The same or similar label correspond to the same or similar components in the attached drawing of the embodiment of the present invention;It is retouched in of the invention
In stating, it is to be understood that if there is the orientation or positional relationship of the instructions such as term " on ", "lower", "left", "right", "front", "rear"
To be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description of the present invention and simplification of the description, rather than indicate or
It implies that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore is described in attached drawing
The term of positional relationship only for illustration, is not considered as limiting the invention, for the ordinary skill of this field
For personnel, the concrete meaning of above-mentioned term can be understood as the case may be.
For the portability and sample diverse problems of current up-conversion fluorescence detection field, the present invention provides one kind just
The up-conversion fluorescence detector for taking formula, by image recognition technology to inhomogeneity in different type, replaceable sample bin module
The sample of type is detected, and sample passes through near infrared light source excitation, and light path system collecting signal forms specificity on the detector
Hot spot, the wherein wavelength of the hot spot of different zones, strength distributing information are extracted by processing, reach the list of multiple types sample
Channel, multicenter auto-inspected purpose.
As shown in Figs. 1-2, a kind of portable up-conversion fluorescence detector, including apparatus subject 2 and changeable type sample mould
Block 1, is equipped with main control chip 4 in the apparatus subject 2, described 4 one end of main control chip be connected with for provide converted in excitation it is glimmering
The light source module 5 of the near-infrared laser of light provides 805nm, the multi-wavelengths such as 980nm;The other end is connected with detector 6, described
Main control chip 4 is also connected with battery 10, wireless module 11 and USB charging module 12;
The changeable type sample block 1 is connect by buckle 8 with apparatus subject 2, at 1 liang of apparatus subject 2, sample block
Side design buckle 8, is fixed by buckling 8 forms;Also, as shown in figure 3, sample block 1,2 joint face of apparatus subject are set
Groove, raised snap-in location structure are counted, to guarantee the accuracy of instrument connection, is equipped with sample in the changeable type sample block 1
Product storehouse 7, the sample are inserted into sample bin 7 by the opening of 7 side of sample bin, and the light source module 5 in the apparatus subject 2 passes through
9 connector of optical fiber and one end of sample bin 7 are detachably connected, and the other end of the sample bin 7 can by lens element and detector 6
Dismantling connection;The sample bin 7 of a variety of types of adaptations is provided, it can be for fluorescent chromatographic test paper, PCR pipe, cuvette, array chip etc.
Various samples type is detected, and 9 beam splitting system of optical fiber and detection system of single channel, multichannel are adapted to according to sample type
System, can measure single sample, can also measure integrated multiple samples;
There are many changeable type sample blocks 1, and difference is that different samples is arranged according to different sample types
Light path system in storehouse 7 and sample bin 7, the sample type include fluorescent chromatographic test paper, PCR pipe, cuvette and array core
Piece;
The light path system includes 7 light path system of 7 light path system of transmission-type sample bin and reflection-type sample bin, the transmission
7 light path system of type sample bin is for being adapted to PCR pipe and cuvette sample, and 7 light path system of reflection-type sample bin is for being adapted to
Fluorescent chromatographic test paper and array chip sample;
As shown in figure 4,7 light path system of transmission-type sample bin includes the light connecting with 9 connector of optical fiber of light source module 5
Fine 9 optical splitters, further include optical filter, the first lens group and reflecting mirror, and light source splits into a series of wait by force by 9 optical splitter of optical fiber
Light source filters off incident light through optical filter after sample, then reduces spot signal by the first lens group, then by anti-
Mirror is penetrated to be reflected into detector 6;
As shown in figure 5,7 light path system of reflection-type sample bin is placed on sample including the second lens group, in 45 degree of angles
On prism, further include optical filter, the third lens group and reflecting mirror, light source amplifies hot spot by the second lens group, shine
It penetrates on prism, by exciting fluorescence in refraction by prism to sample, fluorescence, which passes through, filters off incident light on prismatic reflection to optical filter,
Spot signal is reduced using the third lens group, is reflected into detector 6 by reflecting mirror;
It include convex lens and photosensitive element in the detector 6, for collecting fluorescence signal and the imaging of sample excitation;
The main control chip 4 is used to receive the sample fluorescence signal image-forming information of photosensitive element acquisition and carries out image recognition
And processing, calculate sample concentration.
The sample bin 7 is multichannel form, and including multiple sample cells for placing sample, the sample cell is relative to sample
7 position of storehouse is fixed, then the fluorescence signal for the electromagnetic radiation being placed in sample cell imaging position on photosensitive element is fixed, i.e., special
Fixed sample cell counter sample is imaged on image specific position and corresponds to special modality always;In the 7 subscript sample product of sample bin
7 type serial number of storehouse, when connecting different sample bins 7, by setting 7 serial number automatic patching system program of sample bin, to identify
The sample serial number of different location hot spot;
No matter sample size is how many in sample bin 7, its counter sample serial number is identified by the method for image recognition, is calculated
Its concentration, system divide channel by the different zones to photosensitive element, concentration results are shown in corresponding channel.
Photosensitive element in the detector 6 is CCD or CMOS photosensitive element, formed on CCD or CMOS photosensitive element
For picture in two distinct types of light path system, the picture of sample is hot spot or band with certain rgb value, passes through image
The range of processing technique identification hot spot:
As shown in fig. 6, carrying out binary conversion treatment to image information first, hot spot and back are identified by OTSU method given threshold
256 brightness degrees of the gray value 0 to 255 of the pixel on image are passed through threshold to obtain spot area information by scape
Value selection is set as 0 or 255, even if the inter-class variance of OTSU algorithm target and two class of background is maximum, it is assumed that prospect and background
Segmentation threshold is t, foreground point accounting w0, mean value u0, background dot accounting w1, mean value u1, image mean value u=w0*u0+w1*u1, establish
Objective function g (t)=w0*(u0-u)2+w1*(u1-u)2, g (t) maximum value is solved, corresponding to t value at this time is optimal threshold;
The region that the gray scale 255 that binary conversion treatment obtains later is carried out to image is sample corresponding region, passes through image
Identification technology carries out frame choosing to the different hot spots in image, and extracts the RGB information of all pixels point in region, RGB letter
The information such as electric current, voltage of TFT circuit in corresponding CCD or CMOS photosensitive element are ceased, the intensity of corresponding light, wavenumber information:
F (x, y, z)=x (R)+y (G)+z (B)
The RGB information for calculating hot spot obtains its optical field distribution, obtains intensity, the wave number distributed intelligence of hot spot;
The wave number of hot spot, strength information have corresponded to the fluorescence signal of sample, pass through a series of the glimmering of the standard items to concentration
Optical signal is detected, and after drawing graticule, can be detected to sample to be tested, and calculate its concentration by graticule.
First lens group and the third lens group include the convex lens and one being arranged by incidence side to outgoing side
A concavees lens, for reducing hot spot, second lens group includes the concavees lens and one being arranged by incidence side to outgoing side
A convex lens, for amplifying hot spot.
Operating area 3 and display area 21 are additionally provided on the apparatus subject 2, the operating area 3 is used for detector
Power supply control is carried out, the display area 21 is for showing sample classification and concentration.
The wireless module 11 be used for pc client or communication of mobile terminal, by data sharing to pc client or movement
Terminal, and detector is controlled by pc client or mobile terminal.
Detection process:
1, counter sample storehouse type is selected according to wanted test sample;
2, counter sample storehouse is fixed on instrument, opens instrument, sample bin serial number is set;
3, the standard sample of various concentration is placed in order in sample bin difference channel, fluorescence intensity;
4, the concentration and fluorescence intensity of according to standard sample draw graticule, are stored in instrument internal;
5, setting graticule is above-mentioned graticule, detects testing sample fluorescence intensity, and instrument provides concentration automatically.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of the technical program, should all be covered in the present invention
Scope of the claims in.
Claims (6)
1. a kind of portable up-conversion fluorescence detector, it is characterised in that: including apparatus subject and changeable type sample block, institute
It states and is equipped with main control chip in apparatus subject, described main control chip one end is connected with for providing the near-infrared of excitation up-conversion fluorescence
The light source module of laser, the other end are connected with detector, and the main control chip is also connected with battery, wireless module and USB charging
Module;
The changeable type sample block is connect by buckle with apparatus subject, and sample is equipped in the changeable type sample block
Storehouse, the sample are inserted into sample bin by the opening of sample bin side, and the light source module in the apparatus subject is connect by optical fiber
One end of head and sample bin is detachably connected, and the other end of the sample bin is detachably connected by lens element and detector;
There are many changeable type sample blocks, difference be to be arranged according to different sample types different sample bin and
Light path system in sample bin, the sample type include fluorescent chromatographic test paper, PCR pipe, cuvette and array chip;
The light path system includes transmission-type sample bin light path system and reflection-type sample bin light path system, the transmission-type sample
Storehouse light path system is for being adapted to PCR pipe and cuvette sample, and the reflection-type sample bin light path system is for being adapted to fluorescent chromatographic
Test paper and array chip sample;
The transmission-type sample bin light path system includes the fiber splitter connecting with the fibre-optical splice of light source module, further includes filter
Mating plate, the first lens group and reflecting mirror, light source split into the intense light sources such as a series of by fiber splitter, penetrate after sample
Optical filter filters off incident light, then reduces spot signal by the first lens group, then be reflected into detector by reflecting mirror;
The prism that the reflection-type sample bin light path system includes the second lens group, is placed in 45 degree of angles on sample further includes
Optical filter, the third lens group and reflecting mirror, light source amplify hot spot by the second lens group, are radiated on prism, pass through
Fluorescence is excited in refraction by prism to sample, fluorescence is by filtering off incident light on prismatic reflection to optical filter, using the third lens
Group reduces spot signal, is reflected into detector by reflecting mirror;
It include convex lens and photosensitive element in the detector, for collecting fluorescence signal and the imaging of sample excitation;
The main control chip is used to receive the sample fluorescence signal image-forming information of photosensitive element acquisition and carries out image recognition and place
Reason calculates sample concentration.
2. portable up-conversion fluorescence detector according to claim 1, it is characterised in that: the sample bin is multichannel
Form, including multiple sample cells for placing sample, the sample cell is fixed relative to sample bin location, is then placed in sample cell
The fluorescence signal of electromagnetic radiation fixed imaging position on photosensitive element, i.e., specific sample cell counter sample is imaged on image
Specific position corresponds to special modality always;Show sample bin type serial number in the sample bin subscript, when connecting different sample bins,
By setting sample bin serial number automatic patching system program, to identify the sample serial number of different location hot spot;
No matter sample size is how many in sample bin, its counter sample serial number is identified by the method for image recognition, it is dense to calculate its
Degree, system divide channel by the different zones to photosensitive element, concentration results are shown in corresponding channel.
3. portable up-conversion fluorescence detector according to claim 1, it is characterised in that: photosensitive in the detector
Element is CCD or CMOS photosensitive element, and imaging is in two distinct types of light path system on CCD or CMOS photosensitive element
In, the picture of sample is hot spot or band with certain rgb value, the range of hot spot is identified by image processing techniques:
Binary conversion treatment is carried out to image information first, hot spot and background are identified by OTSU method given threshold, to obtain light
256 brightness degrees of the gray value 0 to 255 of the pixel on image are set as 0 by threshold value selection by macular area domain information
Or 255, even if the inter-class variance of OTSU algorithm target and two class of background is maximum, it is assumed that the segmentation threshold of prospect and background is t, preceding
Sight spot accounting w0, mean value u0, background dot accounting w1, mean value u1, image mean value u=w0*u0+w1*u1, establish objective function g (t)=
w0*(u0-u)2+w1*(u1-u)2, g (t) maximum value is solved, corresponding to t value at this time is optimal threshold;
The region that the gray scale 255 that binary conversion treatment obtains later is carried out to image is sample corresponding region, passes through image recognition
Technology carries out frame choosing to the different hot spots in image, and extracts the RGB information of all pixels point in region, RGB information pair
The information such as the electric current of TFT circuit, voltage in CCD or CMOS photosensitive element are answered, the intensity of corresponding light, wavenumber information:
F (x, y, z)=x (R)+y (G)+z (B)
The RGB information for calculating hot spot obtains its optical field distribution, obtains intensity, the wave number distributed intelligence of hot spot;
The wave number of hot spot, strength information have corresponded to the fluorescence signal of sample, are believed by a series of fluorescence of the standard items to concentration
It number is detected, after drawing graticule, sample to be tested can be detected, and its concentration is calculated by graticule.
4. portable up-conversion fluorescence detector according to claim 1, it is characterised in that: first lens group and
Three lens groups include the convex lens and a concavees lens being arranged by incidence side to outgoing side, described for reducing hot spot
Second lens group includes the concavees lens and a convex lens being arranged by incidence side to outgoing side, for amplifying hot spot.
5. portable up-conversion fluorescence detector according to claim 1, it is characterised in that: also set on the apparatus subject
There are operating area and display area, the operating area is used to carry out power supply control to detector, and the display area is for showing
Sample category not and concentration.
6. portable up-conversion fluorescence detector according to claim 1, it is characterised in that: the wireless module be used for
Pc client or communication of mobile terminal, by data sharing to pc client or mobile terminal, and eventually by pc client or movement
End controls detector.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111398240A (en) * | 2020-05-21 | 2020-07-10 | 中国科学院重庆绿色智能技术研究院 | Fluorescence detection adapter for up-conversion of superlens |
CN113092428A (en) * | 2021-04-02 | 2021-07-09 | 安图实验仪器(郑州)有限公司 | Multiple fluorescence detection method, device, equipment and system |
CN114441497A (en) * | 2022-02-24 | 2022-05-06 | 重庆尚立仪器设备有限公司 | Heavy metal ion detection method based on quantum dot fluorescent probe array |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120280144A1 (en) * | 2011-03-29 | 2012-11-08 | Guilfoyle Richard A | Optical system enabling low power excitation and high sensitivity detection of near infrared to visible upconversion phoshors |
CN103901190A (en) * | 2014-04-17 | 2014-07-02 | 武汉纽康度生物科技有限公司 | IF (immunofluorescence) quantitative analysis meter |
CN107884571A (en) * | 2016-09-30 | 2018-04-06 | 张磊 | Portable fluorescence immunity analysis instrument and Portable fluorescence analysis method |
CN107941771A (en) * | 2017-12-13 | 2018-04-20 | 西安交通大学 | A kind of modularization up-conversion fluorescence chromatographic test paper excitation apparatus |
CN108132346A (en) * | 2017-12-30 | 2018-06-08 | 天津博硕东创科技发展有限公司 | A kind of Portable fluorescence immunity analysis instrument |
CN207908517U (en) * | 2018-03-27 | 2018-09-25 | 中国人民解放军第三〇二医院 | Portable upper forwarding light immunity analysis instrument |
CN109696404A (en) * | 2017-10-20 | 2019-04-30 | 西南大学 | Portable multi-channel detector |
-
2019
- 2019-09-03 CN CN201910829513.6A patent/CN110487767B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120280144A1 (en) * | 2011-03-29 | 2012-11-08 | Guilfoyle Richard A | Optical system enabling low power excitation and high sensitivity detection of near infrared to visible upconversion phoshors |
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