CN103376249A - Ultraviolet point fluorescence scanner - Google Patents
Ultraviolet point fluorescence scanner Download PDFInfo
- Publication number
- CN103376249A CN103376249A CN2012101598367A CN201210159836A CN103376249A CN 103376249 A CN103376249 A CN 103376249A CN 2012101598367 A CN2012101598367 A CN 2012101598367A CN 201210159836 A CN201210159836 A CN 201210159836A CN 103376249 A CN103376249 A CN 103376249A
- Authority
- CN
- China
- Prior art keywords
- ultraviolet
- light
- controller
- scanning instrument
- fluorescent scanning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides an ultraviolet point fluorescence scanner which comprises a light source, a beam-splitting plate, a focusing mirror, an ultraviolet detector and a controller, wherein the controller controls the light source to emit ultraviolet light; the ultraviolet light is reflected to the focusing mirror for focusing through the beam-splitting plate; the focused ultraviolet light is projected to a test strip of a sample with an ultraviolet fluorescent mark, and reflected light is generated; the ultraviolet detector acquires the reflected light, converts the reflected light into an electric signal and transmits the electric signal to the controller; the controller analyzes the concentration of a target object in the sample through the intensity of the reflected light. The diagnostic sensitivity can be effectively improved, and the concentration of the target object can be quantitatively analyzed.
Description
Technical field
The present invention relates to a kind of diagnostic instrments, relate in particular to a kind of ultraviolet point fluorescent scanning instrument.
Background technology
Immunochromatographic method is a kind of quick diagnosis technology of rising in recent years, its principle is a certain region of special antibody being fixed in first specific test paper, after test paper is invaded sample (urine or serum), corresponding antigen and this antibody generation specific binding in the sample, utilize the collaurum developing technology can make test paper show certain color, can be negative or the positive by judgement sample by color.
But there is defective in this technology: the one, and sensitivity is low, then can't develop the color if sample concentration is low; The 2nd, can't be quantitative, although developed in recent years the colour developing densitometer, quantitative poor effect.
Summary of the invention
And defective that can't quantitative low for present immunochromatographic method sensitivity the invention provides a kind of ultraviolet point fluorescent scanning instrument, sensitivity that can the Effective Raise diagnosis, and can carry out quantitative test.
A kind of ultraviolet point fluorescent scanning instrument comprises: light source is used for providing ultraviolet light;
Controller is connected with light source, is used for the control light source and sends ultraviolet light;
Light splitting piece and focus lamp, light splitting piece are arranged on the light path of light source, and focus lamp is arranged on the light splitting optical path of light splitting piece, and light splitting piece is used for ultraviolet light light splitting with light source to focus lamp, and focus lamp is used for the light splitting of light splitting piece is focused on;
Ultraviolet detector is connected with controller, is used for the reflected light of the Ultraluminescence mark generation of sample on the collection test strips, reflected light is converted to electric signal and sends to controller, for the concentration of controller according to target detection thing in the electric signal analytic sample.
Further, ultraviolet point fluorescent scanning instrument also comprises the mobile device for Ultraluminescence mark in many places on the scanning test strips, mobile device comprises stepper motor, screw mandrel, base plate and the sample stage that is used for the carrying test strips, stepper motor and sample stage are arranged on the base plate, stepper motor links to each other with sample stage by screw mandrel, and controller passes through the control step electric machine rotation with the displacement on Quality control platform and the ultraviolet detector generation horizontal direction.
Further, ultraviolet point fluorescent scanning instrument also comprises the first optical filter, and the first optical filter is arranged between light source and the light splitting piece.
Further, ultraviolet point fluorescent scanning instrument also comprises the second optical filter, and the second optical filter is arranged between light splitting piece and the ultraviolet detector.
Further, controller comprises the USB interface of carrying out data transmission for external computing machine.
Further, controller also comprises the printer interface for external printer.
Further, controller also comprises for carrying out the network port of real-time Communication for Power with external system.
Further, adopt cable to be connected between light source and the controller.
A kind of ultraviolet point fluorescent scanning instrument provided by the invention, after focusing on, ultraviolet light that light source sends projects to on the test paper of the sample of Ultraluminescence mark and produce reflected light, ultraviolet detector gathers reflected light and sends to controller, controller is by the concentration of object in the catoptrical intensive analysis sample, concentration by object can be positive or feminine gender by judgement sample, can effectively improve the sensitivity of diagnosis, and can carry out quantitative test to the target detection substrate concentration.
Description of drawings
Fig. 1 is the structural representation of a kind of embodiment of a kind of ultraviolet point fluorescent scanning instrument provided by the invention.
Embodiment
Embodiments of the invention are described with reference to the accompanying drawings.The element of describing in an accompanying drawing of the present invention or a kind of embodiment and feature can combine with element and the feature shown in one or more other accompanying drawings or the embodiment.Should be noted that in order to know purpose, omitted expression and the description of parts that have nothing to do with the present invention, known to persons of ordinary skill in the art and processing in accompanying drawing and the explanation.
As shown in Figure 1, a kind of ultraviolet point fluorescent scanning instrument comprises:
Light source 1 is used for providing ultraviolet light;
The ultraviolet light wavelength is between 200-400nm, and this concept is as well known to those skilled in the art.
Object in sample ultraviolet light mark, object concentration characterizes by intensity of reflected light.
Consider more than one of fluorescence band on the test strips 7, therefore ultraviolet point fluorescent scanning instrument also is provided with the mobile device for Ultraluminescence mark in many places on the scanning test strips 7, mobile device comprises stepper motor 11, screw mandrel 12, base plate 10 and the sample stage 8 that is used for carrying test strips 7, stepper motor 11 and sample stage 8 are arranged on the base plate 10, stepper motor 11 links to each other with sample stage 8 by screw mandrel 12, and controller 13 rotates the displacement that produces on the horizontal direction with Quality control platform 8 and ultraviolet detector 3 by control step motor 11.
Preferably, ultraviolet point fluorescent scanning instrument comprises that also the first optical filter 2, the first optical filters 2 are arranged between light source 1 and the light splitting piece 5, and mating plate 2 can obtain the ultraviolet light of required wavelength after filtration.
Preferably, ultraviolet point fluorescent scanning instrument comprises that also the second optical filter 4, the second optical filters 4 are arranged between light splitting piece 5 and the ultraviolet detector.
Preferably, controller 13 comprises the usb 14 that carries out data transmission for external computing machine.
Preferably, controller 13 also comprises the printer interface 15 for external printer, and external printer is used in real time output measurement data.
Preferably, controller 13 also comprises for carrying out the network port 17 of real-time Communication for Power with external system.
Preferably, adopt cable 16 to be connected between light source 1 and the controller 13.
Below further set forth principle of work of the present invention by specific embodiment.
Object in the sample is carried out ultraviolet marking by the ultraviolet marking technology, afterwards with sample drop on test strips 7, test strips 7 is fixed on the sample stage 8, sample stage 8 links to each other with stepper motor 11 by leading screw 12, stepper motor 11 and sample stage 8 are arranged on the base plate 10, controller 13 control light sources 1 send ultraviolet light, successively through the first optical filter 2, get to the Ultraluminescence mark of test strips 7 after light splitting piece 5 and the focus lamp 6, produce reflected light, reflected light passes through focus lamp 6 successively, received by ultraviolet detector 3 after light splitting piece 5 and the second optical filter 4, ultraviolet detector 3 converts the reflected light that receives to electric signal and sends to controller 13, controller 13 is analyzed catoptrical intensity according to the electric signal that receives, draw the concentration of object in the sample on the test strips 7 by catoptrical intensity, can be negative or the positive by judgement sample according to concentration, the data such as target detection substrate concentration size can show by external printer output.
In addition, controller 13 also can be controlled stepper motor 11 with the displacement that Quality control platform 8 and ultraviolet detector 3 produce on the horizontal direction, makes ultraviolet detector 3 can scan the Ultraluminescence mark of many places on the test strips 7.
A kind of Ultraluminescence scanner provided by the invention, after focusing on, ultraviolet light that light source sends projects to on the test paper of the sample of Ultraluminescence mark and produce reflected light, ultraviolet detector gathers reflected light and sends to controller, controller is by the concentration of target detection thing in the catoptrical intensive analysis sample, concentration by the target detection thing can be positive or feminine gender by judgement sample, can effectively improve the sensitivity of diagnosis, and can carry out quantitative test to the target detection substrate concentration, thereby can be early warning and the fast detecting of some disease, checking on and import and export inspection and quarantine in the scene of food security provides technical guarantee.
Although described the present invention and advantage thereof in detail, be to be understood that and in the situation that does not exceed the spirit and scope of the present invention that limited by appended claim, can carry out various changes, alternative and conversion.And the application's scope is not limited only to the specific embodiment of the described process of instructions, equipment, means, method and step.The one of ordinary skilled in the art will readily appreciate that from disclosure of the present invention, can use according to the present invention carry out with the essentially identical function of corresponding embodiment described herein or obtain result essentially identical with it, existing and want exploited process, equipment, means, method or step future.Therefore, appended claim is intended to comprise such process, equipment, means, method or step in their scope.
Claims (8)
1. a ultraviolet point fluorescent scanning instrument is characterized in that, described ultraviolet point fluorescent scanning instrument comprises:
Light source is used for providing ultraviolet light;
Controller is connected with described light source, is used for controlling described light source and sends ultraviolet light;
Light splitting piece and focus lamp, described light splitting piece is arranged on the light path of described light source, described focus lamp is arranged on the light splitting optical path of described light splitting piece, described light splitting piece is used for ultraviolet light light splitting with described light source to described focus lamp, and described focus lamp is used for the light splitting of described light splitting piece is focused on;
Ultraviolet detector, be connected with described controller, the reflected light that is used for the Ultraluminescence mark generation of sample on the collection test strips, convert described reflected light to electric signal and send to described controller, analyze the concentration of target detection thing in the described sample for described controller according to described electric signal.
2. ultraviolet point fluorescent scanning instrument according to claim 1, it is characterized in that, described ultraviolet point fluorescent scanning instrument also comprises the mobile device for Ultraluminescence mark in many places on the scanning test strips, described mobile device comprises stepper motor, screw mandrel, base plate and the sample stage that is used for the carrying test strips, described stepper motor and described sample stage are arranged on the described base plate, described stepper motor links to each other with sample stage by screw mandrel, and described controller rotates with the displacement on Quality control platform and the ultraviolet detector generation horizontal direction by the described stepper motor of control.
3. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that, described ultraviolet point fluorescent scanning instrument also comprises the first optical filter, and described the first optical filter is arranged between described light source and the described light splitting piece.
4. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that, described ultraviolet point fluorescent scanning instrument also comprises the second optical filter, and described the second optical filter is arranged between described light splitting piece and the described ultraviolet detector.
5. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that described controller comprises the USB interface of carrying out data transmission for external computing machine.
6. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that described controller also comprises the printer interface for external printer.
7. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that, described controller also comprises for carrying out the network port of real-time Communication for Power with external system.
8. ultraviolet point fluorescent scanning instrument according to claim 1 is characterized in that, adopts cable to be connected between described light source and the described controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101598367A CN103376249A (en) | 2012-04-28 | 2012-05-22 | Ultraviolet point fluorescence scanner |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210133097.4 | 2012-04-28 | ||
| CN201210133097 | 2012-04-28 | ||
| CN2012101598367A CN103376249A (en) | 2012-04-28 | 2012-05-22 | Ultraviolet point fluorescence scanner |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103376249A true CN103376249A (en) | 2013-10-30 |
Family
ID=49461697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101598367A Pending CN103376249A (en) | 2012-04-28 | 2012-05-22 | Ultraviolet point fluorescence scanner |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103376249A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104730000A (en) * | 2015-04-13 | 2015-06-24 | 大连理工大学 | Photoelectric quantitative testing device for immune chromatography reaction results |
| CN105548165A (en) * | 2015-12-29 | 2016-05-04 | 南京洁态环保科技有限公司 | Test paper reflectometer and measuring method for test paper surface |
| CN105954276A (en) * | 2016-06-02 | 2016-09-21 | 上海丰能医药科技有限公司 | Intelligent health detection device and method |
| CN108548782A (en) * | 2018-04-18 | 2018-09-18 | 易尚明天科技有限公司 | The active detection method of 5'-NT and kinetics quantitative detection instrument |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030103662A1 (en) * | 2001-12-05 | 2003-06-05 | Finkbeiner Steven M. | Robotic microscopy systems |
| CN1637407A (en) * | 2003-12-30 | 2005-07-13 | 三星电子株式会社 | Fluorescence detector for detecting microfluid |
| CN202693475U (en) * | 2012-04-28 | 2013-01-23 | 北京网新易尚科技有限公司 | Ultraviolet spot fluorescent scanner |
-
2012
- 2012-05-22 CN CN2012101598367A patent/CN103376249A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030103662A1 (en) * | 2001-12-05 | 2003-06-05 | Finkbeiner Steven M. | Robotic microscopy systems |
| CN1637407A (en) * | 2003-12-30 | 2005-07-13 | 三星电子株式会社 | Fluorescence detector for detecting microfluid |
| CN202693475U (en) * | 2012-04-28 | 2013-01-23 | 北京网新易尚科技有限公司 | Ultraviolet spot fluorescent scanner |
Non-Patent Citations (2)
| Title |
|---|
| R.S.思德普尔: "《现代分析仪器手册》", 31 December 1986, 机械工业出版社 * |
| 卢健 等: "上转换发光免疫试纸条扫描检测系统研究", 《光子学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104730000A (en) * | 2015-04-13 | 2015-06-24 | 大连理工大学 | Photoelectric quantitative testing device for immune chromatography reaction results |
| CN105548165A (en) * | 2015-12-29 | 2016-05-04 | 南京洁态环保科技有限公司 | Test paper reflectometer and measuring method for test paper surface |
| CN105548165B (en) * | 2015-12-29 | 2021-01-08 | 南京洁态环保科技有限公司 | Test paper reflectometer and test paper surface measuring method |
| CN105954276A (en) * | 2016-06-02 | 2016-09-21 | 上海丰能医药科技有限公司 | Intelligent health detection device and method |
| CN105954276B (en) * | 2016-06-02 | 2018-12-21 | 上海丰能医药科技有限公司 | A kind of intelligent health detection device and method |
| CN108548782A (en) * | 2018-04-18 | 2018-09-18 | 易尚明天科技有限公司 | The active detection method of 5'-NT and kinetics quantitative detection instrument |
| CN108548782B (en) * | 2018-04-18 | 2020-12-04 | 易尚明天科技有限公司 | Detection method of 5' -nucleotidase activity and reaction kinetics quantitative detector |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106093011B (en) | Coal quality detecting method and its coal quality laser detection analysis instrument of application | |
| CN100526883C (en) | Reflection photometer of gold-labeled immunity test paper strip | |
| WO2005019811A3 (en) | Time dependent fluorescence measurements | |
| CN104535481B (en) | imaging flow cytometer | |
| CN103376249A (en) | Ultraviolet point fluorescence scanner | |
| US9255884B2 (en) | Fluorescence strip, fluorescence excitation device and portable fluorescence analysis system with the same | |
| WO2005086586A2 (en) | Method and system for monitoring material separation process such as electrophoresis process in a sample | |
| CN101608998A (en) | Multiparameter water quality quick detection device based on the ARM technology | |
| CN103454256B (en) | Near-infrared fluorescent scanner | |
| CN104111241A (en) | Linear scanning-based fluorescence confocal detection device | |
| CN106092997B (en) | Flame detecting device and method based on PLIF technology | |
| CN202693475U (en) | Ultraviolet spot fluorescent scanner | |
| CN109253997B (en) | Raman tomography system based on frequency modulation and spatial coding | |
| JP5298684B2 (en) | Foreign object detection device and detection method | |
| CN106338499A (en) | Element laser detection and analysis instrument and mineral element analysis method | |
| CN110530783B (en) | Lateral beam collection method and device for flow cytometer and flow cytometer | |
| CN103454298A (en) | Microbeam X-ray fluorescence analytical method | |
| CN2938080Y (en) | Reflection photometer of gold-labeled immunity test paper strip | |
| CN202814874U (en) | Near-infrared fluorescent scanner | |
| CN103528984A (en) | Optical grating type near infrared analyzer | |
| CN207396349U (en) | A kind of colloidal gold chromatographic card interpretoscope | |
| CN210863540U (en) | Rhodamine detection device based on fluorescence spectrum characteristics | |
| EP4047364A1 (en) | System and method for measuring water characteristics in a water facility | |
| CN109187480A (en) | A kind of peanut oil doping soybean oil discriminating gear of double excitation LIF technology | |
| CN108982431B (en) | On-line fluorescence detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131030 |
|
| RJ01 | Rejection of invention patent application after publication |