CN104730000A - Photoelectric quantitative testing device for immune chromatography reaction results - Google Patents

Abstract

A photoelectric quantitative testing device for immune chromatography reaction results comprises a white light-emitting diode, a photosensitive control triode, a photosensitive testing triode, a micro-screw stepping motor, a micro-spot formation structure, a micro-spot illumination testing structure and a control system. The white light-emitting diode gives out white light for testing; the photosensitive control triode is used for testing and adjusting light intensity of the white light-emitting diode to enable the light intensity to reach a preset value; the micro-spot formation structure is used for enabling scattering while light to form micro-shaped spots 0.8-1.0mm in diameter; the micro-spot illumination testing structure is used for shielding interference of other rays and allowing only reflected light of the micro-shaped spots to pass through; the photosensitive testing triode is used for testing intensity of the reflected light of the micro-shaped spots for analysis and calculation; the micro-screw stepping motor is used for driving other parts to move along the horizontal direction stably and uniformly to have the testing range fully covered, so that states of 1-5 testing lines (T-line) can be detected, and concentration of each testing line (T-line) is analyzed and calculated.

Description

A kind of photoelectricity quantification detection apparatus of immunochromatography reaction result

Technical field

The invention belongs to the pick-up unit of biochemical reaction, the industry fields such as pharmacy, chemical industry, biopreparate, Food Inspection, animal quarantine and hospital assay can be widely used in.It, according to the immunoreactive principle of Ag-Ab, utilizes modern photoelectric detecting technology can check the presence or absence of appointment 1 ~ 5 kind of material and the height of concentration accurately and efficiently

Background technology

Often some micro-biochemicals can be detected by the immunoreactive principle of Ag-Ab at industry fields such as pharmacy, chemical industry, biopreparate, Food Inspection, animal quarantine and hospital assay.Usually the method for test card is used to detect at present, namely instill solution to be measured in one end of test card, solution to be measured makes to be imprinted on specific antibodies colour developing originally colourless on test card paper tape by the paper tape in test card, and (the C line that test card instills the backward end colour developing of solution to be measured is fixing appearance.Colour developing T line is detection line, and whether and relatively the depth of its appearance C line color represents the presence or absence of measured matter or the height of concentration), and the concentration level of measured material is determined according to the shade of colour developing T line.Determine that the depth of test card reaction color roughly determines the presence or absence of detected predetermined substance or the height of concentration by the method for artificial contrast's standard color comparison card at present; Some producers also develop relevant detecting instrument and carry out adjunct test, but current used instrument exists the deficiencies such as the comparatively large and accuracy of detection of metrical error between detection speed slower, same sample duplicate detection error larger, same sample multiple stage instrument is lower.

The present invention is directed to above-mentioned deficiency and adopt modern mechanical, photoelectric detecting technology, carry out optoelectronic scanning to employing Ag-Ab immune response principle test card, the data analysis obtained by optoelectronic scanning obtains result.The error that the method can be avoided manual intervention and produce, has simultaneously and detects the advantage quick, between repetitive error and platform, error is less, greatly facilitate the use in relevant industries, field.

Summary of the invention

The present invention is directed to the industry fields such as current pharmacy, chemical industry, biopreparate, Food Inspection, animal quarantine and hospital assay detect some micro-biochemical ways and meanses deficiency by the immunoreactive principle of Ag-Ab, utilize modern photoelectric detecting technology provide a kind of safe, convenient, use pick-up unit simple, with low cost, with the object reached fast, accurately detect

Technical solution of the present invention is achieved in that

A kind of photoelectricity quantification detection apparatus of immunochromatography reaction result, comprise detecting portion and control section, described detecting portion comprises white light emitting diode, control phototriode, detection phototriode, low-light spot formation structure and low-light spot luminance detection structure; Described control section comprises miniature leading screw stepper motor, guides optical axis, leading screw and control system.The centerlines that low-light spot forms structure and low-light spot luminance detection structure is best between 15 ° ~ 60 °.

White light emitting diode inserts low-light spot and forms structure, and low-light spot forms micro-shape hot spot of the scatter white formation diameter 0.8 ~ 1.0 millimeter that white light emitting diode sends by structure.

Detect phototriode and insert low-light spot luminance detection structure; The interference of other direction light of low-light spot luminance detection structual shield, only allows that the reflected light of micro-shape hot spot passes through.

Controlling phototriode for regulating the luminous intensity of white light emitting diode, making it to reach default value; Detect phototriode and be responsible for the intensity of reflected light detecting miniature hot spot, and analyze by detecting the intensity of reflected light of micro-shape hot spot to C line and T line, calculate the immunoreactive degree of Ag-Ab.

Miniature leading screw stepper motor be responsible under control of the control system driving detecting portion along guide optical axis on leading screw in the horizontal direction steadily, uniform motion, reach all standing to maximum 75mm sensing range with the precision of 0.03mm/ step, and the state of 1 ~ 5 T line can be detected; Control system is responsible for overall motion control, detection of reflected light intensity, analysis, the immunoreactive degree of calculating Ag-Ab and the work such as to communicate.

The present invention can adaptive various test card, and detection speed is fast, accuracy of detection is high, repeatability is strong, and reliability is higher.

Accompanying drawing explanation

Fig. 1 is structural representation of the present invention.

In figure: 1. white light emitting diode; 2. phototriode is controlled; 3. phototriode is detected; 4. miniature leading screw stepper motor; 5. low-light spot forms structure; 6. low-light spot luminance detection structure; 7. test card insert port; 8. optical axis is guided; 9. leading screw;

Fig. 2 is detecting portion (A)-(A) sectional view.

Embodiment

By test card from insertion, control system lights white light emitting diode, and control system detects the luminous intensity of white light emitting diode by controlling phototriode, and regulates the luminous intensity of white light emitting diode to reach predetermined threshold; Control system controls miniature leading screw stepper motor and rotates, and the precision driving whole testing agency to walk with 0.03mm/ carries out horizontal reciprocating movement; In motion process, white light emitting diode forms by low-light spot micro-shape hot spot that structure forms diameter 0.8 ~ 1.0 millimeter on test card, detect the intensity of reflected light of phototriode by the micro-shape hot spot of low-light spot luminance detection structure detection, and analyze by detecting the intensity of reflected light of micro-shape hot spot to C line and T line, calculate the immunoreactive degree of Ag-Ab.

Control system is responsible for overall motion control, detection of reflected light intensity, analysis, the immunoreactive degree of calculating Ag-Ab and the work such as to communicate.

Claims (2)

1. the photoelectricity quantification detection apparatus of an immunochromatography reaction result, comprise detecting portion and control section, it is characterized in that, described detecting portion comprises white light emitting diode, control phototriode, detection phototriode, low-light spot formation structure and low-light spot luminance detection structure; Described control section comprises miniature leading screw stepper motor, guides optical axis, leading screw and control system;

White light emitting diode inserts low-light spot and forms structure, and low-light spot forms micro-shape hot spot of the scatter white formation diameter 0.8 ~ 1.0 millimeter that white light emitting diode sends by structure;

Detect phototriode and detect phototriode and all insert low-light spot luminance detection structure; The interference of other direction light of low-light spot luminance detection structual shield, only allows that the reflected light of micro-shape hot spot passes through;

Controlling phototriode for regulating the luminous intensity of white light emitting diode, making it to reach default value; Detect phototriode and be responsible for the intensity of reflected light detecting miniature hot spot, and analyze by detecting the intensity of reflected light of micro-shape hot spot to C line and T line, calculate the immunoreactive degree of Ag-Ab;

Miniature leading screw stepper motor be responsible under control of the control system driving detecting portion along guide optical axis on leading screw in the horizontal direction steadily, uniform motion, reach all standing to maximum 75mm sensing range with the precision of 0.03mm/ step, and the state of 1 ~ 5 T line can be detected;

Control system is responsible for overall motion control, detection of reflected light intensity, analysis, the immunoreactive degree of calculating Ag-Ab and the work such as to communicate.

2. the photoelectricity quantification detection apparatus of a kind of immunochromatography reaction result according to claim 1, is characterized in that, low-light spot forms the centerlines of structure and low-light spot luminance detection structure between 15 ° ~ 60 °.