CN217516962U - Tilting cell culture vibrates mixing arrangement - Google Patents
Tilting cell culture vibrates mixing arrangement Download PDFInfo
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- CN217516962U CN217516962U CN202220213530.4U CN202220213530U CN217516962U CN 217516962 U CN217516962 U CN 217516962U CN 202220213530 U CN202220213530 U CN 202220213530U CN 217516962 U CN217516962 U CN 217516962U
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Abstract
The utility model discloses a tilting cell culture vibrates mixing arrangement, include the heat-preserving container and set up the cell culture section of thick bamboo in the heat-preserving container, the bottom of cell culture section of thick bamboo sets up the inclined plane board that the slope was arranged into, wherein: the left end and the right end of the cell culture cylinder are respectively movably connected with the heat preservation barrel at the same side through the driven vibrating component and the driving vibrating component, the outer side wall of the heat preservation barrel is provided with a driving motor, and the driving motor is connected with the driving vibrating component through a transmission mechanism. The utility model adopts the structural design that the heat-preserving barrel and the cell culture barrel sleeve are arranged, and the heat-preserving barrel and the cell culture barrel sleeve are respectively connected through the driven vibrating component and the driving vibrating component, so as to realize the up-and-down and left-and-right vibration of the cell culture barrel, the mixing effect is good, and the bottom of the cell culture barrel is arranged to be an inclined plane, thereby being more beneficial to the growth of adherent cells; and its structural design is simple, novel, uses labour saving and time saving, has reduced experiment operating personnel's manually operation's work load, has improved work efficiency.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a tilting cell culture vibrates mixing arrangement.
Background
Cell culture is an essential process in bioengineering technology, which can be performed by mass culture of a single cell into simple single cells or rarely differentiated multiple cells. A large number of cells or their metabolites can be obtained by cell culture. Since most biological products are derived from cells, cell culture techniques are the most central and fundamental techniques in biotechnology. Cell culture work is widely applied to various fields such as biology, medicine, new medicine research and development and the like, and plays a vital role in the whole life science research.
When the experimenter inoculates the cells in the culture container, the cells are added into the culture container together with the liquid culture medium, and the cells are in a suspension state in the culture medium, so the cells inoculated into the culture container are uniformly distributed in the container along with the liquid culture medium. After standing for a period of time, according to the difference of the types of the accessed cells, some cells will grow adherent to the wall, and some cells will grow in suspension, the cells growing adherent to the wall will be evenly distributed and attached to the bottom of the container, and the cells growing in suspension will be evenly distributed in the culture medium in the container.
In the cell culture process, in order to improve the mixing uniformity of the inoculated cells and other added drugs such as a culture medium, a shaking mixing device is required to be used for inoculating the cells into a liquid culture medium and continuously shaking for culture, which is relatively speaking of static culture or surface culture of mold. Meanwhile, the contact between cells and culture medium components and the supply of oxygen can be improved, the propagation is uniform, and particularly, a mycoderm and a small ball formed by long hyphae cannot be formed in the culture of the mould.
However, the existing shaking mixing device has a single structure, and in the shaking process, cells cannot be quickly and fully mixed with a liquid culture medium, so that the culture quality of the cells is influenced, and therefore the existing requirements are not met.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to the above-mentioned defect that exists among the prior art, provide a pollution risk little, can once cultivate the tilting cell culture vibration mixing arrangement who harvests a large amount of cells, the high just sealing performance of convenience in use is good, stable in structure.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a tilting cell culture vibrates mixing arrangement, including the heat-preserving container with set up in a cell culture section of thick bamboo in the heat-preserving container, the bottom of a cell culture section of thick bamboo sets up the inclined plane board of arranging for the slope, wherein: the cell culture section of thick bamboo about both ends respectively through driven shock subassembly and initiative vibrations subassembly swing joint homonymy the heat-preserving container, just the heat-preserving container outer wall is provided with driving motor, driving motor passes through drive mechanism and connects the initiative vibrations subassembly.
Further, on the inclined cell culture shaking mixing device, the driven shaking assembly comprises:
the driven sliding chute is arranged at one side end of the cell culture cylinder;
the driven sliding block is movably embedded in the driven sliding groove;
the lower end of the driven upper pressure spring is connected with the top of the driven sliding block, and the upper end of the driven upper pressure spring is abutted to the groove in the top of the driven sliding groove;
the upper end of the driven lower pressure spring is connected with the bottom of the driven sliding block, and the lower end of the driven lower pressure spring abuts against the groove in the bottom of the driven sliding chute; and
the driven connecting shaft, the one end of driven connecting shaft is inlayed and is located the shaft hole of driven slider, the other end is connected the inside wall of heat-preserving container.
Further preferably, on the inclined cell culture shaking mixing device, the driven chute is disposed at a middle position of the side wall of the cell culture cylinder, and one side end of the inclined plate close to the driven chute is a high end.
Further, on the inclined cell culture shaking mixing device, the active shaking assembly comprises:
the active chute is arranged at the other side end of the cell culture cylinder;
the driving sliding block is movably embedded in the driving sliding groove;
the lower end of the driving upper pressure spring is connected with the top of the driving sliding block, and the upper end of the driving upper pressure spring is abutted to the groove in the top of the driving sliding groove;
the upper end of the driving lower pressure spring is connected with the bottom of the driving sliding block, and the lower end of the driving lower pressure spring is abutted to the groove at the bottom of the driving sliding chute; and
and one end of the driving connecting shaft is fixedly connected with the driving sliding groove, and the other end of the driving connecting shaft is connected with the driving motor through the transmission mechanism.
Further preferably, on the inclined cell culture shaking mixing device, the active chute is disposed at a middle position of the sidewall of the cell culture cylinder, and a side end of the inclined plate close to the active chute is a lower end.
Further preferably, on the inclined cell culture shaking mixing device, the transmission mechanism comprises:
the upper end of the transmission connecting rod is fixedly connected with one end of the driving connecting shaft, and the lower end of the transmission connecting rod is provided with a buffer hole along the length direction;
the edge position of the eccentric gear is provided with a fixed column which is embedded in the buffer hole; and
and the driving gear is in meshing connection with the eccentric gear, and a central shaft is connected with an output shaft of the driving motor.
Further preferably, on the inclined cell culture oscillation mixing device, the transmission mechanism is arranged in a transmission bin body on the outer side wall of the heat preservation barrel, and a limiting hole for accommodating the driving connecting shaft is formed in the inner side wall of the transmission bin body.
Further preferably, on the inclined cell culture shaking mixing device, the eccentric gear is movably arranged on the outer side wall of the transmission bin body through a coaxially arranged rotating shaft.
Furthermore, on the tilting cell culture shake mixing arrangement, the heat preservation section of thick bamboo with form between the cell culture section of thick bamboo with the heat preservation chamber, heat preservation chamber bottom is provided with inlet tube, drain pipe, level gauge and temperature probe.
Further preferably, on the inclined cell culture shaking mixing device, a PCL controller is arranged on the upper part of the heat-preserving barrel, and the PCL controller is electrically connected with the electric heater, the liquid level meter, the temperature probe and the driving motor.
The above technical scheme is adopted in the utility model, compared with the prior art, following technological effect has:
(1) the structure design that the heat-preserving barrel and the cell culture barrel are sleeved is adopted, and the heat-preserving barrel and the cell culture barrel are connected through the driven vibrating component and the driving vibrating component respectively, so that the up-and-down and left-and-right vibration of the cell culture barrel is realized, the uniformly mixing effect is good, and the bottom of the cell culture barrel is arranged to be an inclined plane, so that the growth of adherent cells is facilitated;
(2) the driven vibrating components and the driving vibrating components arranged on the left side and the right side are buffered by the upper spring and the lower spring, so that the stability of the cell culture solution in the cell culture cylinder is ensured, and the severe vibration is avoided; the driven vibrating component and the driving vibrating component are respectively arranged between the heat-insulating barrel and the cell culture barrel in a sealing manner, so that the transmission mechanism is fully hidden and protected, and the operation safety is improved;
(3) the active vibration component is used as a power mechanism for realizing the unilateral vibration device, and the active connecting shaft is utilized to realize the left-right swinging vibration and the up-down vibration of the cell culture cylinder along with the eccentric gear, so that the multi-dimensional vibration of the cell culture cylinder is realized; and structural design is simple, novel, uses labour saving and time saving, has reduced experiment operating personnel's manually operation's work load, has improved work efficiency.
(4) The structural design that the heat-insulating barrel and the cell culture barrel are sleeved is adopted, and a heat-insulating cavity is directly formed between the heat-insulating barrel and the cell culture barrel, so that the temperature stability of cell culture is ensured; the liquid level and the temperature of the heating circulating water are monitored in real time through a liquid level meter and a temperature probe, and automatic temperature control and vibration are realized through a PCL controller fixed on the heat-preserving barrel.
Drawings
FIG. 1 is a schematic view showing a three-dimensional structure of an inclined cell culture shaking mixing device of the present invention;
FIG. 2 is a schematic view of a second three-dimensional structure of the tilting cell culture shaking mixing device of the present invention;
FIG. 3 is a schematic view of the inclined cell culture shaking and mixing device according to the present invention;
FIG. 4 is a schematic view of a top view of the tilting cell culture shaking mixing device of the present invention;
FIG. 5 is a schematic diagram of a cross-sectional structure of an inclined cell culture shaking mixing device of the present invention;
FIG. 6 is a schematic diagram of a cross-sectional structure of a slant type cell culture shaking mixing device of the present invention;
FIG. 7 is a schematic diagram of a cross-sectional structure of a tilting cell culture shaking mixing device of the present invention;
FIG. 8 is a fourth schematic view of a cross-sectional structure of the slant type cell culture shaking mixing device of the present invention;
FIG. 9 is a first schematic structural view of an active vibration assembly and a transmission structure of the slant type cell culture shaking mixing device of the present invention;
FIG. 10 is a schematic structural view of an active vibration assembly and a transmission structure of the slant type cell culture shaking mixing device of the present invention;
FIG. 11 is a schematic longitudinal sectional view of the slant type cell culture shaking mixing device of the present invention;
FIG. 12 is a schematic diagram of a longitudinal sectional structure of an inclined cell culture shaking mixing device according to the present invention;
wherein the reference symbols are:
1-a heat preservation barrel, 2-a cell culture cylinder, 3-a heat preservation cavity, 4-a bevel board, 5-a water inlet pipe, 6-a water outlet pipe, 7-a liquid level meter, 8-a temperature probe, 9-a driven sliding groove, 10-a driven sliding block, 11-a driven upper pressure spring, 12-a driven lower pressure spring, 13-a driven connecting shaft, 14-a transmission bin, 15-a mounting seat, 16-a driving motor, 17-a driving sliding groove, 18-a driving sliding block, 19-a driving upper pressure spring, 20-a driving lower pressure spring, 21-a driving connecting shaft, 22-a limiting hole, 23-a transmission connecting rod, 24-a buffer hole, 25-an eccentric gear, 26-a fixed column, 27-a driving gear, 28-an electric heater and 29-a PCL controller.
Detailed Description
The present invention will be described in detail and specifically with reference to specific embodiments so as to provide a better understanding of the present invention, but the following embodiments do not limit the scope of the present invention.
In some embodiments, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, an inclined cell culture shaking mixing device is provided, which includes a thermal insulation barrel 1, a cell culture barrel 2 disposed in the thermal insulation barrel 1, and a thermal insulation chamber 3 formed therebetween, wherein the bottom of the cell culture barrel 2 is provided with an inclined plate 4 disposed obliquely, the arrangement of the inclined plate 4 is more favorable for the growth of adherent cells, and the thermal insulation chamber 3 has a certain thermal insulation effect.
This unilateral oscillation device's main technical scheme is, will both ends are respectively through driven vibration subassembly and initiative vibration subassembly swing joint homonymy about cell culture section of thick bamboo 2 heat-preserving container 1, just 1 lateral wall of heat-preserving container is provided with driving motor 16, driving motor 16 passes through drive mechanism and connects initiative vibration subassembly, initiative vibration subassembly is as the one end that initiative vibration mechanism drove cell culture section of thick bamboo 2 and vibrations about reaching, and the driven vibration subassembly reciprocates along with cell culture section of thick bamboo 2 under cell culture section of thick bamboo 2's vibrations effect, plays certain buffering and cushioning effect.
Specifically, as shown in fig. 5, 6, 7 and 8, the driven oscillating assembly mainly comprises a driven sliding chute 9, a driven sliding block 10, a driven upper pressure spring 11, a driven lower pressure spring 12 and a driven connecting shaft 13, and is integrally sealed.
Wherein, the driven chute 9 is arranged at one side end of the cell culture cylinder 2, the driven chute 9 is a square chute hole, in order to prevent the culture solution in the cell culture cylinder 2 from entering the driven chute 9 in the oscillation process, one side end of the driven chute 9 close to the cell culture cylinder 2 is a closed structure and is not communicated with the cell culture cylinder 2; the driven sliding groove 9 is arranged in the middle of the side wall of the cell culture cylinder 2, and one side end of the inclined plane plate 4 close to the driven sliding groove 9 is a high end.
The driven slide block 10 is movably embedded in the driven slide groove 9, and a matched guide slide groove or guide slide rail can be arranged in the driven slide groove 9 or on the driven slide block 10 for ensuring the stability of the driven slide block 10 sliding up and down in the driven slide groove 9.
In addition, for improving the stability of vibrations, avoid violent excessive vibrations, still set up the buffer structure who comprises driven upper pressure spring 11 and driven lower pressure spring 12 in driven spout 9. The lower end of the driven upper pressure spring 11 is connected with the top of the driven sliding block 10, the upper end of the driven upper pressure spring abuts against the groove in the top of the driven sliding groove 9, the upper end of the driven lower pressure spring 12 is connected with the bottom of the driven sliding block 10, and the lower end of the driven lower pressure spring abuts against the groove in the bottom of the driven sliding groove 9.
Connect a cell culture section of thick bamboo 2 and heat-preserving container 1 through driven connecting axle 13, the one end of driven connecting axle 13 is passed through the sealing washer and is inlayed and locate driven slider 10's shaft hole, the other end is connected the inside wall of heat-preserving container 1. In the up-and-down and left-and-right vibration process of the cell culture cylinder 2, the driven connecting shaft 13 fixed on the heat preservation barrel 1 plays a role in supporting the driven vibration component.
As shown in fig. 5, 6, 7, 8, 9 and 10, the active vibration component mainly comprises an active sliding groove 17, an active sliding block 18, an active upper compression spring 19, an active lower compression spring 20 and an active connecting shaft 21, and is integrally sealed.
Wherein, the active chute 17 is arranged at the other side end of the cell culture cylinder 2; the active chute 17 is a square chute hole, in order to prevent the culture solution in the cell culture cylinder 2 from entering the active chute 17 in the oscillation process, one side end of the active chute 17 close to the cell culture cylinder 2 is of a closed structure and is not communicated with the cell culture cylinder 2; the active sliding chute 17 is disposed at the middle position of the sidewall of the cell culture cylinder 2, and one side end of the inclined plane plate 4 close to the active sliding chute 17 is a lower end.
The driving slide block 18 is movably embedded in the driving slide groove 17; in order to ensure the stability of the vertical sliding of the active sliding block 18 in the active sliding slot 17, a guiding sliding slot or a guiding sliding rail can be arranged in the active sliding slot 17 or on the active sliding block 18.
In addition, in order to improve the stability of vibration and avoid severe excessive vibration, a buffer structure consisting of an active upper pressure spring 19 and an active lower pressure spring 20 is arranged in the active sliding groove 17. The lower end of the driving upper pressure spring 19 is connected with the top of the driving sliding block 18, and the upper end of the driving upper pressure spring is abutted to the groove in the top of the driving sliding groove 17; the upper end of the active lower compression spring 20 is connected with the bottom of the active sliding block 18, and the lower end of the active lower compression spring abuts against the groove in the bottom of the active sliding groove 17.
Connect a cell culture section of thick bamboo 2 and heat-preserving container 1 through initiative connecting axle 21, the one end fixed connection of initiative connecting axle 21 in initiative spout 17, the other end passes through drive mechanism connects driving motor 16 can drive the horizontal hunting of a cell culture section of thick bamboo 2 in step through the rotation of initiative connecting axle 21, can realize that a cell culture section of thick bamboo 2 reciprocates simultaneously. The driving connecting shaft 21 is driven by the driving motor 16 to rotate and move up and down, so that the up-and-down and left-and-right swinging of the cell culture cylinder 2 is synchronously realized, and the aim of multi-dimensional oscillation of the cell culture cylinder 2 is fulfilled.
In addition, as shown in fig. 5, 6, 7, 8, 9 and 10, the driving motor 16 drives the driving connecting shaft 21 to rotate and move up and down through a transmission mechanism, and the transmission mechanism is designed to have an eccentric structure and mainly comprises a transmission connecting rod 23, an eccentric gear 25 and a driving gear 27.
The upper end of the transmission connecting rod 23 is fixedly connected with one end of the driving connecting shaft 21, and the lower end of the transmission connecting rod 23 is provided with a buffer hole 24 along the length direction; the edge position of the eccentric gear 25 is provided with a fixing column 26, and the eccentric gear 25 is movably arranged on the outer side wall of the transmission bin body 14 through a coaxially arranged rotating shaft. The fixing column 26 is embedded in the buffer hole 24; the driving gear 27 is engaged with the eccentric gear 25, and the central shaft is connected with the output shaft of the driving motor 16.
In the rotation process of the eccentric gear 25, the fixed column 26 drives the lower end of the transmission connecting rod 23 to move circumferentially with the axis of the eccentric gear 25 as the center of circle, and synchronously, the upper end of the transmission connecting rod 23 moves up and down and swings left and right in the rotation process, so as to drive the cell culture cylinder 2 to swing up and down and swing left and right, thereby realizing the oscillation purpose.
It should be noted that, in order to avoid the horizontal movement of the active vibration component in the vibration process, the active vibration component only moves in the up-down direction, the transmission mechanism is disposed in the transmission bin 14 on the outer side wall of the heat-insulating bucket 1, the inner side wall of the transmission bin 14 is provided with a limiting hole 22 for accommodating the active connecting shaft 21, and the active connecting shaft 21 is limited by the limiting hole 22, so that the active connecting shaft 21 only moves up and down along the active connecting shaft 21 while rotating under the driving of the driving transmission mechanism.
In addition, as shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 11 and fig. 12, the structure design of the heat preservation barrel 1 and the cell culture barrel 2 is adopted, and the heat preservation cavity 3 is directly formed between the heat preservation barrel 1 and the cell culture barrel 2, so that the stability of the temperature of the cell culture is ensured; the heat preservation cavity 3 at the bottom of the heat preservation barrel 1 can adopt air to carry out heat insulation or adopt an electric heating sleeve to carry out heat preservation. In a preferred embodiment, the inside of the soaking chamber 3 is kept warm by using circulating water, and therefore, an electric heater 28 is provided in the soaking chamber 3, and the circulating water injected into the soaking chamber 3 is heated by the electric heater 28.
Wherein, the side wall of the heat-preserving container 1 is also provided with a water inlet pipe 5, a water outlet pipe 6, a liquid level meter 7 and a temperature probe 8. And a PCL controller 29 is arranged on the upper part of the heat-preserving barrel 1, and the PCL controller 29 is electrically connected with the electric heater 28, the liquid level meter 7, the temperature probe 8 and the driving motor 16. The liquid level and the temperature of circulating water in the heat preservation cavity 3 can be monitored in real time through the liquid level meter 7 and the temperature probe 8, and automatic temperature control and oscillation mixing operation are realized through the PCL controller 29 fixed on the heat preservation barrel 1.
The driven vibrating component and the driving vibrating component which are arranged on the left side and the right side of the utility model ensure the stability of the cell culture solution vibration in the cell culture cylinder 2 under the buffering of the upper spring and the lower spring, and avoid the generation of violent vibration; the driven vibrating component and the driving vibrating component are respectively arranged between the heat-insulating barrel 1 and the cell culture barrel 2 in a sealing way, so that the transmission mechanism is fully hidden and protected, and the operation safety is improved; the driving vibration component is used as a power mechanism of the unilateral vibration device, and the driving connecting shaft 21 is utilized to realize the left-right swinging vibration and the up-down vibration of the cell culture cylinder 2 along with the eccentric gear 26, so that the multi-dimensional vibration of the cell culture cylinder 2 is realized; and its structural design is simple, novel, uses labour saving and time saving, has reduced experiment operating personnel's manually operation's work load, has improved work efficiency.
The above detailed description of the embodiments of the present invention is only for exemplary purposes, and the present invention is not limited to the above described embodiments. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Therefore, the equivalent changes and modifications without departing from the spirit and scope of the present invention should be covered in the scope of the present invention.
Claims (10)
1. The utility model provides a tilting cell culture vibrates mixing arrangement, its characterized in that, include a heat preservation section of thick bamboo (1) and set up in a cell culture section of thick bamboo (2) in a heat preservation section of thick bamboo (1), the bottom of a cell culture section of thick bamboo (2) sets up to slope board (4) of arranging, wherein: cell culture section of thick bamboo (2) about both ends respectively through driven vibration subassembly and initiative vibrations subassembly swing joint homonymy a section of thick bamboo (1) keeps warm, just a section of thick bamboo (1) lateral wall is provided with driving motor (16), driving motor (16) are connected through drive mechanism the subassembly is shaken in the initiative.
2. The inclined cell culture shaking mixing device of claim 1, wherein the driven shaking assembly comprises:
the driven sliding chute (9), the said driven sliding chute (9) is opened in one side end of the said cell culture tube (2);
the driven sliding block (10), the driven sliding block (10) is movably embedded in the driven sliding groove (9);
the lower end of the driven upper pressure spring (11) is connected with the top of the driven sliding block (10), and the upper end of the driven upper pressure spring (11) is abutted to the groove in the top of the driven sliding groove (9);
the upper end of the driven lower pressure spring (12) is connected with the bottom of the driven sliding block (10), and the lower end of the driven lower pressure spring (12) is abutted to the groove in the bottom of the driven sliding groove (9); and
the driven connecting shaft (13), the one end of driven connecting shaft (13) is inlayed and is located the shaft hole of driven slider (10), the other end is connected the inside wall of a heat preservation section of thick bamboo (1).
3. The inclined cell culture shaking mixing device as defined in claim 2, wherein the driven chute (9) is disposed at a middle position of the side wall of the cell culture cylinder (2), and a side end of the inclined plate (4) near the driven chute (9) is a high end.
4. The inclined cell culture shaking mixing device of claim 1, wherein the active shaking assembly comprises:
the active chute (17), the active chute (17) is arranged at the other side end of the cell culture cylinder (2);
the driving sliding block (18), the driving sliding block (18) is movably embedded in the driving sliding groove (17);
the lower end of the driving upper compression spring (19) is connected with the top of the driving sliding block (18), and the upper end of the driving upper compression spring (19) is abutted to the groove in the top of the driving sliding groove (17);
the upper end of the active lower pressure spring (20) is connected with the bottom of the active sliding block (18), and the lower end of the active lower pressure spring (20) abuts against the groove in the bottom of the active sliding groove (17); and
the driving connecting shaft (21), one end of the driving connecting shaft (21) is fixedly connected with the driving sliding groove (17), and the other end of the driving connecting shaft is connected with the driving motor (16) through the transmission mechanism.
5. The inclined cell culture shaking mixing device as claimed in claim 4, wherein the active chute (17) is disposed at a middle position of the side wall of the cell culture cylinder (2), and a side end of the inclined plate (4) close to the active chute (17) is a lower end.
6. The inclined cell culture shaking mixing device of claim 4, wherein the transmission mechanism comprises:
the upper end of the transmission connecting rod (23) is fixedly connected with one end of the driving connecting shaft (21), and the lower end of the transmission connecting rod (23) is provided with a buffer hole (24) along the length direction;
the edge position of the eccentric gear (25) is provided with a fixed column (26), and the fixed column (26) is embedded in the buffer hole (24); and
the driving gear (27) is in meshing connection with the eccentric gear (25), and a central shaft is connected with an output shaft of the driving motor (16).
7. The inclined cell culture shaking mixing device as defined in claim 6, wherein the transmission mechanism is disposed in a transmission chamber (14) on the outer sidewall of the thermal insulation cylinder (1), and a limiting hole (22) for accommodating the active connecting shaft (21) is formed on the inner sidewall of the transmission chamber (14).
8. The inclined cell culture shaking mixing device as claimed in claim 7, wherein the eccentric gear (25) is movably arranged on the outer side wall of the transmission chamber body (14) through a coaxially arranged rotating shaft.
9. The inclined cell culture shaking mixing device as claimed in claim 1, wherein a heat preservation cavity (3) is formed between the heat preservation cylinder (1) and the cell culture cylinder (2), an electric heater (28) is arranged at the bottom of the heat preservation cavity (3), and a water inlet pipe (5), a water outlet pipe (6), a liquid level meter (7) and a temperature probe (8) are arranged on the side wall of the heat preservation cavity.
10. The inclined cell culture shaking mixing device as claimed in claim 9, wherein a PCL controller (29) is arranged on the upper part of the heat preservation cylinder (1), and the PCL controller (29) is electrically connected with the electric heater (28), the liquid level meter (7), the temperature probe (8) and the driving motor (16).
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CN202220213530.4U CN217516962U (en) | 2022-01-26 | 2022-01-26 | Tilting cell culture vibrates mixing arrangement |
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CN202220213530.4U CN217516962U (en) | 2022-01-26 | 2022-01-26 | Tilting cell culture vibrates mixing arrangement |
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