CN217484137U - An online detection device for marine algae based on flow cytometry analysis - Google Patents

Abstract

The utility model discloses a marine algae on-line detection device based on flow cytometry analysis, relating to the technical field of algae detection; the device comprises a sampling device fixedly arranged at the seaside, wherein the sampling device is communicated with a sample processing device, one side of the sample processing device is communicated with a flow cytometer, and the flow cytometer is electrically connected with a data processing device; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model provides a marine algae on-line measuring device based on flow cytometry can accomplish unmanned on duty continuous monitoring, realizes the automatic acquisition and the preliminary treatment of marine water sample simultaneously, effectively improves the mobility that detects.

Description

An online detection device for marine algae based on flow cytometry analysis

technical field

The utility model relates to the technical field of algae detection, in particular to an on-line detection device for marine algae based on flow cytometry analysis.

Background technique

The commonly used algae detection method in China is to collect algae samples artificially on site, fix them and bring them back to the laboratory for observation using microscope imaging technology. Microscopic imaging methods include optical microscopy, electron microscopy, and fluorescence microscopy. In the field of existing monitoring technologies for algae, such as microscopy, extraction, and on-site fluorescence methods, unattended continuous monitoring cannot be achieved; remote sensing methods require the purchase of data, which is greatly affected by weather; wireless video monitoring buoys are difficult to obtain Reliable biological data; while the fluorescent monitoring buoy is easily affected by the density of dissolved organic matter and algae in the water body, so it is impossible to obtain reference data.

Utility model content

The purpose of the utility model is to provide an on-line detection device for marine algae based on flow cytometry analysis, so as to solve the problems existing in the above-mentioned prior art, and to achieve unattended continuous monitoring.

For achieving the above object, the utility model provides the following scheme:

The utility model provides an on-line detection device for marine algae based on flow cytometry analysis, comprising a sampling device fixedly arranged on the seashore, the sampling device is connected with a sample processing device, and a flow cytometer is connected with one side of the sample processing device , the flow cytometer is electrically connected with a data processing device, and the flow cytometer refers to that the cells (or other particles) pass through the laser beam at high speed in a single manner, collect various signals generated when the cells are illuminated, and analyze the signals. An instrument for processing and correlation analysis of various parameters. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out the specified cell subsets according to the preselected parameter range; The sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model can realize the combination of cell individual analysis and high-frequency automatic in-situ monitoring. At the same time, automatic collection and preprocessing of water samples are realized, which effectively improves the mobility of detection.

Optionally, the sampling device includes a first sampling pump and a second sampling pump that are respectively communicated with the ocean through pipelines, the first sampling pump is communicated with the sample processing device through a first reversing valve, and the The second sampling pump is communicated with the sample processing device through the second reversing valve, and the two sampling pumps can sample the ocean separately or simultaneously, thereby realizing continuous and stable sampling.

Optionally, the sample processing device includes a sample tank that communicates with the sampling device, a magnetic stirrer is provided at the bottom of the sample tank, an ultrasonic instrument is communicated in the sample tank, and one side of the sample tank is connected to the sample tank. The flow cytometer is connected; the magnetic stirrer and the ultrasonic instrument are respectively connected with the control device; the ultrasonic instrument can break the cells in the sample tank, and the magnetic stirrer can stir them. Furthermore, the accuracy of monitoring the biophysical and biochemical characteristic parameters of single cells can be improved.

Optionally, a waste liquid treatment device is connected to the lower side of the sample tank through a solenoid valve; an overflow liquid storage device is connected to the upper side of the sample tank through an overflow valve, which can effectively discharge the collected water samples therefrom. , to avoid collecting too many water samples and overflowing the device, and the collected water samples can also be discharged after monitoring and processed uniformly, so that the water samples collected again can be stored.

Optionally, an overflow liquid processing device is connected between the first sampling pump and the first reversing valve through an overflow valve; and an overflow liquid is connected between the second sampling pump and the second reversing valve. The overflow valve is connected with an overflow liquid treatment device, and the overflow valve avoids nowhere to carry after sampling too much, and improves the safety and accuracy.

Optionally, the pipelines between the first reversing valve and the second reversing valve and the sample processing device are all connected with a flow regulating valve, and the flow regulating valve is connected with a cleaning liquid storage device through a gear pump; The gear pump is connected with the control device, and the sample tank and the sampling device can be cleaned through the flow regulating valve and the gear pump.

The utility model has achieved the following technical effects with respect to the prior art:

The utility model combines the sampling device and the sample processing device, so that the flow cytometer technology can be carried out from the laboratory to the field, and the algae can be detected, analyzed and automatically identified more quickly. Realize remote, online, long-term monitoring of algae, and improve the mobility of monitoring. In the utility model, the collected water samples are pretreated by dispersing and stirring, and then the flow cytometer can be used to monitor the biophysical and biochemical characteristic parameters of single cells in real time, and the data processing device can analyze the parameters that can be detected in real time. The data for reference can be used to facilitate further analysis, judgment and preventive measures, so as to achieve early warning of harmful algal blooms. The cleaning device provided in the utility model can clean the entire monitoring system, ensure the accuracy of the in-situ data obtained by monitoring the collected water samples each time, and help prevent the occurrence of harmful algal blooms in time. The utility model has a simple structure, can satisfy real-time release of processed data, can dynamically monitor the dynamics of algae in the ocean anytime and anywhere, and realize real early warning of harmful algae.

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only of the present invention. For some embodiments of the present invention, for those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

Fig. 1 is the layout schematic diagram of the marine algae online detection device based on flow cytometry analysis of the present invention;

Among them, 100 is an online detection device for marine algae based on flow cytometry analysis, 1 is a flow cytometer, 2 is a data processing device, 3 is a first sampling pump, 4 is a second sampling pump, and 5 is a first reversing valve , 6 is the second reversing valve, 7 is the sample tank, 8 is the magnetic stirrer, 9 is the ultrasonic instrument, 10 is the control device, 11 is the solenoid valve, 12 is the waste liquid treatment device, 13 is the overflow valve, and 14 is the The overflow liquid storage device, 15 is an overflow liquid processing device, 16 is a flow regulating valve, 17 is a gear pump, and 18 is a cleaning liquid storage device.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

The purpose of the utility model is to provide an on-line detection device for marine algae based on flow cytometry analysis, so as to solve the problems existing in the above-mentioned prior art, and to achieve unattended continuous monitoring.

In order to make the above objects, features and advantages of the present utility model more clearly understood, the present utility model will be described in further detail below with reference to the accompanying drawings and specific embodiments.

Referring to FIG. 1 , the present invention provides an online detection device 100 for marine algae based on flow cytometry analysis, which includes a device 100 that is fixedly installed on the seashore and can be used in a preset time range, preset location or preset water source. A sampling device for collecting water samples to be tested within a depth range, the sampling device is connected with a sample processing device, one side of the sample processing device is connected with a flow cytometer 1, the flow cytometer 1 is electrically connected with a data processing device 2, and the flow cytometer 1 is electrically connected with a data processing device 2. Cytometer 1 refers to an instrument that enables cells (or other particles) to pass through a laser beam at high speed in a single manner, collects various signals generated when cells are illuminated, processes the signals, and performs correlation analysis of various parameters. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out the specified cell subsets according to the preselected parameter range; The utility model flow cytometer extracts the pretreated sample water source from the sample tank at a set pump speed, and detects while sampling, and completes the number of algae, algal cell length, size, shape, granularity, pigment for the extracted sample water source. , peak number, algae population characteristics and other parameters are detected and the corresponding pulse map is drawn; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model can realize the combination of cell individual analysis and high-frequency automatic in-situ monitoring. At the same time, the method of automatic collection and pretreatment of water samples is realized, which effectively improves the mobility of monitoring.

Specifically, the sampling device includes a first sampling pump 3 and a second sampling pump 4 which are respectively communicated with the ocean through pipelines. The first sampling pump 3 communicates with the sample processing device through a first reversing valve 5 , and the second sampling pump 6 The second reversing valve 6 is communicated with the sample processing device, and the two sampling pumps can sample the ocean separately or at the same time, thereby realizing continuous and stable sampling. The interface of the sampling pump is a copper tube, and the built-in hose peristaltic pump replaces the gear pump. , can prevent seawater corrosion. The sample processing device comprises a sample tank 7 communicated with the sampling device, the bottom of the sample tank 7 is provided with a magnetic stirrer 8, the sample tank 7 is communicated with an ultrasonic instrument 9, and one side of the sample tank 7 is connected with the flow cytometer 1; 8 and the ultrasonic instrument 1 are respectively connected to the control device 10; the magnetic stirrer 8 stirs the water source sample located in the sample tank 7 to ensure the uniformity of the sample water source and avoid the floating of algae in the water sample to affect the accuracy of the CytoSense flow cytometer detection The ultrasonic 9 instrument ultrasonically vibrates the water source sample in the sample tank 7 through the horn connected to it, so as to achieve the purpose of fully dispersing the algae, and as far as possible to break up large groups of algae into small groups or even monomers. The lower side of the sample tank 7 is connected to a waste liquid treatment device 12 through a solenoid valve 11; the upper side of the sample tank 7 is connected to an overflow liquid storage device 14 through an overflow valve 13, which can effectively discharge the collected water samples from it, avoiding If there are too many water samples collected and overflow the device, the collected water samples can also be discharged after monitoring and processed uniformly, so that the water samples collected again can be stored. An overflow liquid processing device 15 is connected between the first sampling pump 3 and the first reversing valve 5 through the overflow valve 13; between the second sampling pump 4 and the second reversing valve 6, an overflow valve 13 is connected. The flow liquid processing device 15, through the overflow valve 13, avoids no place to carry after sampling too much, and improves the safety and accuracy. The pipelines between the first reversing valve 5 and the second reversing valve 6 and the sample processing device are all connected to the flow regulating valve 16, and the flow regulating valve 16 is connected to the cleaning liquid storage device 18 through the gear pump 17; The control device 10 is connected, and the sample tank 7 and the sampling device can be cleaned through the flow regulating valve 16 and the gear pump 17 .

In the description of the present invention, it should be noted that the terms "center", "top", "bottom", "left", "right", "vertical", "horizontal", "inner" and "outer" The orientation or positional relationship indicated by etc. is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, with a specific orientation. Therefore, it should not be construed as a limitation on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

The principles and implementations of the present utility model are described with specific examples in the present utility model, and the descriptions of the above embodiments are only used to help understand the method and the core idea of the present utility model; meanwhile, for those skilled in the art , according to the idea of the present utility model, there will be changes in the specific implementation and application scope. In conclusion, the content of this specification should not be construed as a limitation on the present invention.

Claims (6)

1. a marine algae online detection device based on flow cytometry analysis, is characterized in that: comprise the sampling device that is fixedly arranged on the seaside, the sampling device is communicated with a sample processing device, and one side of the sample processing device is communicated with a flow A cytometer, the flow cytometer is electrically connected with a data processing device; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. 2. The marine algae online detection device based on flow cytometry analysis according to claim 1, characterized in that: the sampling device comprises a first sampling pump and a second sampling pump which are respectively communicated with the ocean through pipelines, so The first sampling pump communicates with the sample processing device through a first reversing valve, and the second sampling pump communicates with the sample processing device through a second reversing valve. 3 . The marine algae online detection device based on flow cytometry analysis according to claim 1 , wherein the sample processing device comprises a sample tank communicated with the sampling device, and the bottom of the sample tank is provided with a magnetic stirrer. 4 . The sample tank is connected with an ultrasonic instrument, and one side of the sample tank is connected with the flow cytometer; the magnetic stirrer and the ultrasonic instrument are respectively connected with the control device. 4. The marine algae online detection device based on flow cytometry analysis according to claim 3, characterized in that: the lower side of the sample tank is connected with a waste liquid treatment device through a solenoid valve; the upper side of the sample tank is connected with a waste liquid treatment device The overflow valve is connected to the overflow liquid storage device. 5 . The marine algae online detection device based on flow cytometry analysis according to claim 2 , wherein an overflow liquid is connected between the first sampling pump and the first reversing valve through an overflow valve. 6 . processing device; an overflow liquid processing device is connected between the second sampling pump and the second reversing valve through an overflow valve. 6 . The marine algae online detection device based on flow cytometry analysis according to claim 2 , wherein the pipelines between the first reversing valve and the second reversing valve and the sample processing device are all uniform. 7 . It is connected with a flow regulating valve, and the flow regulating valve is connected with a cleaning liquid storage device through a gear pump; the gear pump is connected with the control device.

Images