CN217484137U - Marine algae on-line detection device based on flow cytometry - Google Patents
Marine algae on-line detection device based on flow cytometry Download PDFInfo
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- CN217484137U CN217484137U CN202220363786.3U CN202220363786U CN217484137U CN 217484137 U CN217484137 U CN 217484137U CN 202220363786 U CN202220363786 U CN 202220363786U CN 217484137 U CN217484137 U CN 217484137U
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Abstract
The utility model discloses a marine algae on-line detection device based on flow cytometry analysis, relating to the technical field of algae detection; the device comprises a sampling device fixedly arranged at the seaside, wherein the sampling device is communicated with a sample processing device, one side of the sample processing device is communicated with a flow cytometer, and the flow cytometer is electrically connected with a data processing device; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model provides a marine algae on-line measuring device based on flow cytometry can accomplish unmanned on duty continuous monitoring, realizes the automatic acquisition and the preliminary treatment of marine water sample simultaneously, effectively improves the mobility that detects.
Description
Technical Field
The utility model relates to an alga detects technical field, especially relates to a marine alga on-line measuring device based on flow cytometry.
Background
The method for detecting algae commonly used in China is to collect algae samples manually on site, fix the algae samples and then bring the algae samples back to a laboratory for observation by using a microscope imaging technology. The microscopic imaging method comprises optical microscopic observation, electron microscopic observation, fluorescence microscopic method and the like. In the existing technical field of monitoring of algae, such as a microscope method, an extraction method, an on-site fluorescence method and the like, unattended continuous monitoring cannot be achieved; the remote sensing method needs to purchase data and is greatly influenced by weather; the wireless video monitoring buoy is difficult to obtain reliable biological data; the fluorescence monitoring buoy is easily influenced by dissolved organic matters and algae density in the water body, and can not obtain reference data.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a marine alga on-line measuring device based on flow cytometry to solve the problem that above-mentioned prior art exists, possible unmanned on duty continuous monitoring.
In order to achieve the above object, the utility model provides a following scheme:
the utility model provides a marine algae on-line measuring device based on flow cytometry, including fixed the setting in the sampling device of seaside, the sampling device intercommunication has sample processing apparatus, sample processing apparatus one side intercommunication has flow cytometer, the flow cytometer electricity is connected with data processing apparatus, and flow cytometer indicates, makes cell (or other particles) pass through laser beam with single mode in proper order at a high speed, gathers the various signals that the cell produced when being shone, handles the signal to an instrument that carries out correlation analysis to each parameter. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to preselected parameter ranges; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model discloses can realize that cell individual analysis and the automatic normal position monitoring of high frequency combine together. Meanwhile, automatic collection and pretreatment of water samples are realized, and the detection maneuverability is effectively improved.
Optionally, the sampling device includes first sampling pump and second sampling pump that communicate through the pipeline respectively with the ocean in, first sampling pump through first switching-over valve with the sample processing apparatus intercommunication, the second sampling pump through the second switching-over valve with the sample processing apparatus intercommunication, two sampling pumps can be respectively or simultaneously to the ocean sample to continuous stable sample has been realized.
Optionally, the sample processing apparatus includes a sample tank communicated with the sampling apparatus, a magnetic stirrer is disposed at the bottom of the sample tank, an ultrasonic apparatus is communicated with the sample tank, and one side of the sample tank is connected with the flow cytometer; the magnetic stirrer and the ultrasonic instrument are respectively connected with the control device; the ultrasonic wave appearance can be to the cell disruption in the sample jar, and magnetic stirrers can stir it. Further, the accuracy of monitoring the characteristic parameters of the unicellular in the aspects of biophysics and biochemistry can be improved.
Optionally, one side below the sample tank is connected with a waste liquid treatment device through an electromagnetic valve; one side above the sample tank is connected with an overflow liquid storage device through an overflow valve, so that collected water samples can be effectively discharged from the overflow liquid storage device, the phenomenon that the collected water samples are too much and overflow devices are avoided, and the collected water samples can be discharged after being monitored and uniformly processed, so that the collected water samples can be stored.
Optionally, an overflow liquid processing device is connected between the first sampling pump and the first reversing valve through an overflow valve; an overflow liquid processing device is connected between the second sampling pump and the second reversing valve through an overflow valve, no bearing is left after excessive sampling is avoided through the overflow valve, and safety and accuracy are improved.
Optionally, pipelines between the first and second reversing valves and the sample processing device are connected to a flow control valve, and the flow control valve is connected to a cleaning solution storage device through a gear pump; the gear pump with controlling means connects, can wash sample tank and sampling device through flow control valve and gear pump.
The utility model discloses for prior art gain following technological effect:
the utility model discloses combine sampling device and sample processing apparatus for the open-air is walked out to the flow cytometer technique from the laboratory, can carry out more swift alga detection and analysis and automatic identification. The algae can be monitored remotely, online and for a long time, and the monitoring mobility is improved. The utility model discloses in through the preliminary treatment of scattering and stirring to gathering the water sample, and then utilize flow cytometer can carry out real-time supervision to the characteristic parameter in the aspect of unicellular biophysics, biochemistry to analyze out the data that can supply the reference by data processing apparatus, and then conveniently carry out analysis judgement and precaution on next step, satisfy and realize early warning to harmful algal bloom. The utility model discloses in through the belt cleaning device who sets up, can rinse whole monitoring system, guarantee at every turn to the accuracy of the water sample monitoring of collection and the normal position data that obtains, help in time preventing the emergence of harmful algal bloom. The utility model discloses simple structure, the satisfying of handling data publishes in real time, reaches the developments of alga in the anytime and anywhere dynamic monitoring ocean, realizes real early warning to harmful alga.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic layout of the on-line marine algae detection device based on flow cytometry;
wherein 100 is a marine algae on-line detection device based on flow cytometry, 1 is a flow cytometer, 2 is a data processing device, 3 is a first sampling pump, 4 is a second sampling pump, 5 is a first reversing valve, 6 is a second reversing valve, 7 is a sample tank, 8 is a magnetic stirrer, 9 is an ultrasonic instrument, 10 is a control device, 11 is an electromagnetic valve, 12 is a waste liquid processing device, 13 is an overflow valve, 14 is an overflow liquid storage device, 15 is an overflow liquid processing device, 16 is a flow control valve, 17 is a gear pump, and 18 is a cleaning liquid storage device.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model aims at providing a marine algae on-line measuring device based on flow cytometry to solve the problem that above-mentioned prior art exists, accomplish unmanned on duty continuous monitoring.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
Referring to the attached drawing 1, the utility model provides a marine algae on-line measuring device 100 based on flow cytometry, including fixed the setting in the seaside, and can be at predetermined time range, the sampling device of the collection of the water sample that awaits measuring in predetermined place or predetermined water source depth range, sampling device intercommunication has sample processing apparatus, sample processing apparatus one side intercommunication has flow cytometer 1, flow cytometer 1 electricity is connected with data processing apparatus 2, flow cytometer 1 indicates, make cell (or other particles) pass through laser beam with single mode high speed in proper order, various signals that produce when gathering the cell by the illumination, handle the signal, and carry out correlation analysis's an instrument to each parameter. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to preselected parameter ranges; the utility model discloses flow cytometer extracts the sample water source through the preliminary treatment from the sample jar with the pump speed of settlement, samples while detecting, accomplishes parameter detection such as the figure of alga, alga cell length, size, form, granularity, pigment, peak number, alga colony characteristic to the sample water source of extraction and draws corresponding pulse map; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device. The utility model discloses can realize that cell individual analysis and the automatic normal position monitoring of high frequency combine together. Meanwhile, the method realizes automatic collection and pretreatment of the water sample, and effectively improves the monitoring maneuverability.
Specifically, sampling device is including respectively with the ocean in through the first sampling pump 3 and the second sampling pump 4 of pipeline intercommunication, first sampling pump 3 is through first switching-over valve 5 and sample processing apparatus intercommunication, second sampling pump 6 is through second switching-over valve 6 and sample processing apparatus intercommunication, two sampling pumps can be respectively or simultaneously to the ocean sample, thereby realized continuous stable sample, the sampling pump kneck is the copper pipe, built-in hose peristaltic pump has replaced the gear pump, can prevent that the sea water from corroding. The sample processing device comprises a sample tank 7 communicated with the sampling device, a magnetic stirrer 8 is arranged at the bottom of the sample tank 7, an ultrasonic instrument 9 is communicated in the sample tank 7, and one side of the sample tank 7 is connected with the flow cytometer 1; the magnetic stirrer 8 and the ultrasonic instrument 1 are respectively connected with a control device 10; the magnetic stirrer 8 stirs the water source sample in the sample tank 7, so that the uniformity of the sample water source is ensured, and the influence of floating of algae in the water sample on the detection accuracy of the Cytosense flow cytometer is avoided; the ultrasonic wave 9 instrument carries out ultrasonic vibration on the water source sample in the sample tank 7 through the amplitude transformer connected with the ultrasonic wave 9 instrument, so as to achieve the purpose of fully dispersing algae, and break up large algae clusters into small clusters and even monomers as far as possible. One side of the lower part of the sample tank 7 is connected with a waste liquid treatment device 12 through an electromagnetic valve 11; one side of the upper part of the sample tank 7 is connected with an overflow liquid storage device 14 through an overflow valve 13, so that collected water samples can be effectively discharged from the overflow liquid storage device, the phenomenon that the collected water samples are too much and overflow devices are avoided, and the collected water samples can be discharged after monitoring and uniformly processed, so that the collected water samples can be stored again. An overflow liquid processing device 15 is connected between the first sampling pump 3 and the first reversing valve 5 through an overflow valve 13; an overflow liquid processing device 15 is connected between the second sampling pump 4 and the second reversing valve 6 through an overflow valve 13, the overflow valve 13 is used for avoiding the situation that no part bears after excessive sampling, and the safety and the accuracy are improved. Pipelines between the first reversing valve 5 and the sample processing device and between the second reversing valve 6 and the sample processing device are both connected with a flow regulating valve 16, and the flow regulating valve 16 is connected with a cleaning liquid storage device 18 through a gear pump 17; the gear pump 17 is connected to the control device 10, and the sample tank 7 and the sampling device can be cleaned by the flow control valve 16 and the gear pump 17.
In the description of the present invention, it should be noted that the terms "center", "top", "bottom", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The utility model discloses a concrete example is applied to explain the principle and the implementation mode of the utility model, and the explanation of the above example is only used to help understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.
Claims (6)
1. The utility model provides a marine algae on-line measuring device based on flow cytometry, its characterized in that: the device comprises a sampling device fixedly arranged at the seaside, wherein the sampling device is communicated with a sample processing device, one side of the sample processing device is communicated with a flow cytometer, and the flow cytometer is electrically connected with a data processing device; the sampling device, the sample processing device and the flow cytometer are respectively connected with a control device.
2. The on-line marine algae detection apparatus based on flow cytometry analysis of claim 1, wherein: the sampling device comprises a first sampling pump and a second sampling pump which are respectively communicated with the interior of the ocean through pipelines, the first sampling pump is communicated with the sample processing device through a first reversing valve, and the second sampling pump is communicated with the sample processing device through a second reversing valve.
3. The on-line marine algae detection apparatus based on flow cytometry analysis of claim 1, wherein: the sample processing device comprises a sample tank communicated with the sampling device, a magnetic stirrer is arranged at the bottom of the sample tank, an ultrasonic instrument is communicated in the sample tank, and one side of the sample tank is connected with the flow cytometer; the magnetic stirrer and the ultrasonic instrument are respectively connected with the control device.
4. The on-line marine algae detection apparatus based on flow cytometry analysis according to claim 3, wherein: one side below the sample tank is connected with a waste liquid treatment device through an electromagnetic valve; and one side above the sample tank is connected with an overflow liquid storage device through an overflow valve.
5. The on-line marine algae detection apparatus based on flow cytometry analysis of claim 2, wherein: an overflow liquid processing device is connected between the first sampling pump and the first reversing valve through an overflow valve; and an overflow liquid processing device is connected between the second sampling pump and the second reversing valve through an overflow valve.
6. The on-line marine algae detection apparatus based on flow cytometry analysis of claim 2, wherein: pipelines between the first reversing valve and the sample processing device and between the second reversing valve and the sample processing device are both connected with a flow regulating valve, and the flow regulating valve is connected with a cleaning liquid storage device through a gear pump; the gear pump is connected with the control device.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220363786.3U CN217484137U (en) | 2022-02-22 | 2022-02-22 | Marine algae on-line detection device based on flow cytometry |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220363786.3U CN217484137U (en) | 2022-02-22 | 2022-02-22 | Marine algae on-line detection device based on flow cytometry |
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| CN217484137U true CN217484137U (en) | 2022-09-23 |
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| CN202220363786.3U Active CN217484137U (en) | 2022-02-22 | 2022-02-22 | Marine algae on-line detection device based on flow cytometry |
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