CN217442968U - Treatment device for proteins and metabolites in biological fluids - Google Patents

Abstract

The utility model relates to a processing apparatus for protein and metabolite in biological fluid, set up multi-functional after-treatment device and membrane adapter membrane elevating gear along a side on the workstation, set up filter screen frame recovery platform on the membrane adapter membrane elevating gear, set up the arm on the workstation, the arm can be at X axle and Y epaxial horizontal migration, set up the gripper on the arm, the front end of gripper sets up liquid-moving gun and grabber, the middle part of workstation sets up the sample frame, one side of sample frame sets up scanning device and suction filtration system, suction filtration system supplies the sample body fluid in the sample test tube to flow into in the membrane adapter through the rotation, suction filtration system and revolving table position correspond, the circumference sets gradually on the revolving table goes up unloading station, suction filtration station, a stoving position and No. two stoving positions, the utility model discloses with manual process automation, the while of multistep concurrent operation has improved work efficiency, has solved cross contamination's problem.

Description

Treatment device for proteins and metabolites in biological fluids

Technical Field

The utility model relates to a processing apparatus for protein and metabolite in biological body fluid, wherein biological body fluid specifically includes liquid such as urine, saliva, people's milk, blood.

Background

Biological specimens are of great value for medical research and medical practice. Biological fluids often contain a wealth of information from patients, where proteins are thought to contain potential markers of kidney disease, and are thought to include information about the entire body. Biological fluids, an important biological sample, if stored with clinical history at every stage of the disease, would play an immeasurable role in future retrospective and prospective studies looking for biomarkers, especially in large-scale clinical validation experiments. However, biological samples in current medical practice are not completely preserved. The reason is that the preservation of the biological body fluid is technically difficult, the biological body fluid is generally preserved by freezing at the temperature of minus 80 ℃, so the preservation greatly increases the energy cost, and the biological sample has relatively low protein concentration, so the volume of the required liquid is large, a large amount of preservation occupies more space, power failure and other conditions occur, the freezing environment cannot be ensured, and the sample can be damaged.

Therefore, in recent years, researchers try to adopt a filter membrane adsorption mode, and the filter membrane can be stored for a long time after being dried. Protein in biological sample liquid is absorbed by adopting a manual suction bottle in a suction filtration manner, but in the operation process, the operation is complicated, the condition of cross contamination is easy to occur, the protein information of the biological body fluid is disordered, and the result judgment is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model provides a processing apparatus for protein and metabolite in biological body fluid, with manual process automation, the simultaneous operation of multistep has simultaneously improved work efficiency, has solved cross contamination's problem, and in the instrument operation process, whole sample can not cross contact with the filter membrane after adsorbing to avoid producing cross contamination, accomplish the accuracy of handling the sample and trace to the source.

In order to realize the purpose, the utility model discloses a technical scheme be:

a processing device for proteins and metabolites in biological fluids, characterized by: a multifunctional post-processing device and a membrane adapter membrane lifting device are arranged on the workbench along one side edge, and a filter screen frame recovery platform is arranged on the membrane adapter membrane lifting device;

the automatic film breaking device comprises a workbench, and is characterized in that a mechanical arm is arranged on the workbench, the mechanical arm can horizontally move on an X axis and a Y axis, a mechanical claw is arranged on the mechanical arm, a plurality of liquid moving guns and grippers are arranged at the front end of the mechanical claw, the grippers contract inwards to grip a film breaking needle and a sample test tube, and the grippers expand outwards to support a film adapter;

the middle part of the workbench is provided with a sample rack, one side of the sample rack is provided with a suction filtration system, the suction filtration system is used for enabling sample body fluid in a sample test tube to flow into the membrane adapter through radial rotation, a sample test tube rotating device, a needle washing device and a scanning device are sequentially arranged between the suction filtration system and the multifunctional post-processing device, the sample test tube rotating device is used for rotating the sample test tube and can enable the scanning device to scan the sample test tube, the suction filtration system corresponds to the rotary workbench in position, and a feeding and discharging station, a suction filtration station, a first drying station and a second drying station are sequentially arranged on the circumference of the rotary workbench;

the control system is in signal connection with the mechanical arm, the mechanical claw, the scanning device, the suction filtration system, the rotary worktable and the multifunctional post-processing device respectively, and the control system can supply the working process to be automated through received signals.

The treatment device for proteins and metabolites in biological fluids, wherein: the pipette is used for extracting the sample body fluid in the sample test tube, and the grabber is used for grabbing the membrane adapter to move to a corresponding position.

The treatment device for proteins and metabolites in biological fluids, wherein: the scanning device can record the sample test tube information, and the suction filtration system supplies suction filtration of the membrane adapter.

The treatment device for proteins and metabolites in biological fluids, wherein: the multifunctional post-processing device is used for spraying and printing information on the code spraying packing paper, is placed in the membrane adapter and can also be used for separating the membrane adapter.

The utility model has the advantages that: with manual process automation, multistep concurrent operation has simultaneously improved work efficiency, has solved cross contamination's problem, and the instrument operation in-process, whole sample can not cross contact with the filter membrane after adsorbing to avoid producing cross contamination, accomplish the accuracy of handling the sample and trace to the source.

Drawings

FIG. 1 is a block diagram of a processing device for proteins and metabolites in biological fluids.

FIG. 2 is a plan view of a processing apparatus for proteins and metabolites in biological fluids.

FIG. 3 is a diagram of a gripper configuration for a device for processing proteins and metabolites in biological fluids.

FIG. 4 is a diagram of a suction filtration system for a treatment device for proteins and metabolites in biological fluids.

FIG. 5 is a schematic view of a rotary table of the apparatus for treating proteins and metabolites in biological fluids.

FIG. 6 is a block diagram of a multifunctional post-treatment device for use with a treatment device for proteins and metabolites in biological fluids.

Description of reference numerals: 1-a workbench; 2, a mechanical arm; 3-mechanical claw; 4-a sample holder; 5-a scanning device; 6-a suction filtration system; 61-sample introduction device; a 62-O type seal ring; 63-a vacuum pump; 7-a rotary table; 71-a support saddle; 72-upright post; 73-a stepper motor; 74-four hole position turntable; 8-a multifunctional post-processing device; 81-a sliding assembly; 82-a functional component; 83-a storage component; 9-membrane adapter membrane lifting device; 10-a screen frame recovery table; 11-a loading and unloading station; 12-a suction filtration station; 13-drying position I; 14-second drying position; 15-sample tube rotation device; 16-a needle washing device; 17-a pipette; 18-a gripper; 19-membrane rupturing needle.

Detailed Description

As shown in fig. 1 to 6, the utility model provides a processing apparatus for protein and metabolite in biological fluid sets up multi-functional after-treatment device 8 and membrane adapter membrane elevating gear 9 along a side on workstation 1, multi-functional after-treatment device 8 includes sliding component 81, functional module 82 and storage module 83, sets up storage module 83 in the sliding component 81, sliding component 81 still with functional module 82 sliding connection, functional module 82 through slide and then adjust with the relative position of storage module 83, functional module 82 can be with spouting the seal information on spouting the sign indicating number packing paper, and place in the membrane adapter, can also supply the membrane adapter to separate, storage module 83 supplies to spout sign indicating number packing paper floating support, can also collect the filter screen support after the separation, set up filter screen frame recovery platform 10 on membrane adapter membrane elevating gear 9, membrane adapter membrane elevating gear 9 provides new membrane adapter, filter screen frame recovery platform 10 collects the filter screen frame after the separation.

The automatic film breaking device is characterized in that a mechanical arm 2 is arranged on the workbench 1, the mechanical arm 2 can horizontally move on an X axis and a Y axis, a mechanical claw 3 is arranged on the mechanical arm 2, a liquid moving gun 17 and a plurality of grippers 18 are arranged at the front end of the mechanical claw 3, the grippers 18 contract inwards to grip a film breaking needle 19 and a sample test tube, and the grippers 18 expand outwards to support a film adapter;

the middle part of workstation 1 sets up sample frame 4, one side of sample frame 4 sets up suction filtration system 6, suction filtration system 6 supplies the interior sample body fluid of sample test tube to flow into through radial rotation in the diaphragm adapter, suction filtration system 6 with set gradually sample test tube rotary device 15 between the multi-functional aftertreatment device 8, wash needle device 16 and scanning device 5, sample test tube rotary device 15 supplies the sample test tube rotatory, and can make scanning device 5 scans sample test tube information, wash needle device 16 can wash after using rupture of membranes needle 19.

Suction filtration system 6 supplies the interior sample body fluid of sample test tube to flow in the diaphragm adapter through radial rotation, suction filtration system 6 passes through pipeline and vacuum pump 63 intercommunication, suction filtration system 6 is through flexible removal and sampling device 61 contact or separation, suction filtration system 6 still through O type sealing washer 62 with diaphragm adapter sealing connection, sampling device 61 supplies through the rotation the interior sample inflow of sample test tube the diaphragm adapter, the diaphragm adapter passes through vacuum pump 63 suction filtration.

The suction filtration system 6 corresponds to the rotary worktable 7, the rotary worktable 7 comprises a support base plate 71, the support base plate 71 is fixed on the worktable through a vertical column 72, a stepping motor 73 is arranged below the support seat plate 71, the support seat plate 71 is fixedly connected with a four-hole turntable 74 through a transition shaft sleeve, the transition shaft sleeve is in transmission connection with the stepping motor 73, the stepping motor 73 is used for the four-hole-position turntable 74 to horizontally rotate, a feeding and discharging station 11, a suction filtration station 12, a first drying station 13 and a second drying station 14 are sequentially arranged on the circumference of the four-hole-position turntable 74, the rotary worktable 7 supplies the diaphragm adapter to sequentially carry out suction filtration and twice drying through rotation, the suction filtration system 6 is arranged below the suction filtration station 12, and a pair of drying assemblies is symmetrically arranged above and below the first drying position 13 and the second drying position 14 respectively.

The control system is in signal connection with the mechanical arm 2, the mechanical claw 3, the scanning device 5, the suction filtration system 6, the rotary worktable 7 and the multifunctional post-processing device 8 respectively, and the control system can supply work flow to be automated through received signals.

A method for preserving a processing device for proteins and metabolites in biological fluids, comprising the steps of:

step 1, a control system moves through a control mechanical arm 2 and drives a mechanical claw 3 to be above a sample frame 4, the mechanical claw 3 clamps a sample test tube on the sample frame 4 through a grabber and is placed on a sample test tube rotating device 15, the sample test tube rotating device 15 is used for rotating the sample test tube and enabling a scanning device 5 to scan the sample test tube, information of the sample test tube is recorded, and after the recording is finished, the mechanical claw 3 transfers the sample test tube to a suction filtration system 6;

step 2, the mechanical claw 3 moves to a membrane adapter membrane lifting device 9, a membrane adapter is expanded outwards through a grabber 18 and supported and placed in a feeding and discharging station 11, the control system starts a rotary workbench 7, the rotary workbench 7 rotates in the radial direction to move the membrane adapter on the feeding and discharging station 11 to a suction filtration station 12, the suction filtration system 6 transfers the sample body fluid in the placed sample test tube into the membrane adapter in two ways, one way is that the suction filtration system 6 pours the sample body fluid in the sample test tube into the membrane adapter through rotation, and the other way is that the sample body fluid in the sample test tube is extracted through a fluid transfer gun 17 at the front end of the mechanical claw 3 and is injected into the membrane adapter;

step 3, after the sample body fluid is added into the membrane adapter, the control system starts the suction filtration system 6 to perform suction filtration process, when the suction filtration is blocked or the suction filtration is incomplete, the membrane breaking needle 19 is required to break the membrane to enable the body fluid to be completely suction filtered, the membrane breaking needle 19 is used up and then is placed in the needle washing device 16 to be cleaned, after the suction filtration operation is completed, the control system controls the rotary workbench 7 to rotate, the membrane adapter after the suction filtration is sequentially moved to a first drying position 13 and a second drying position 14 to perform two drying processes, and after the drying process is completed, the membrane adapter rotates through the rotary workbench 7 to rotate to the feeding and discharging position 11;

step 4, the control system clamps the membrane adapter to a multifunctional post-processing device 8 by controlling the mechanical claw 3, the control system further controls the multifunctional post-processing device 8 to separate the membrane adapter, and simultaneously, code-spraying packing paper is placed on a filtering membrane in a separated filter screen support, the multifunctional post-processing device 8 is not in contact with the filtering membrane, so that cross contamination is avoided, and the separated filter screen support is subjected to vacuum packaging;

and 5, the control system controls the mechanical claw 3 to clamp the filter screen frame left after the membrane adapter is separated, and the filter screen frame is transferred to a filter screen frame recovery platform 10 to carry out a recovery and reuse process.

The utility model has the advantages that:

with manual process automation, multistep simultaneous operation has improved work efficiency, has solved cross contamination's problem simultaneously, and in the instrument operation process, whole sample can not cross contact with the filter membrane after adsorbing to avoid producing cross contamination, accomplish the accuracy of handling the sample and trace to the source.

The foregoing description is intended to be illustrative rather than limiting, and it will be appreciated by those skilled in the art that many modifications, variations or equivalents may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (4)

1. A processing device for proteins and metabolites in biological fluids, characterized by: a multifunctional post-processing device (8) and a membrane adapter membrane lifting device (9) are arranged on one side edge of the upper edge of the workbench (1), and a filter screen frame recovery platform (10) is arranged on the membrane adapter membrane lifting device (9);

the automatic film breaking device is characterized in that a mechanical arm (2) is arranged on the workbench (1), the mechanical arm (2) can horizontally move on an X axis and a Y axis, a mechanical claw (3) is arranged on the mechanical arm (2), a liquid moving gun (17) and a plurality of grabbers (18) are arranged at the front end of the mechanical claw (3), the grabbers (18) contract inwards to clamp a film breaking needle (19) and a sample test tube, and the grabbers (18) expand outwards to support the film adapter;

a sample rack (4) is arranged in the middle of the workbench (1), a suction filtration system (6) is arranged on one side of the sample rack (4), the suction filtration system (6) supplies the sample body fluid in the sample test tube to flow into the membrane adapter through radial rotation, a sample test tube rotating device (15), a needle washing device (16) and a scanning device (5) are sequentially arranged between the suction filtration system (6) and the multifunctional post-processing device (8), the sample test tube rotating device (15) is used for rotating a sample test tube, the scanning device (5) can scan the sample test tube, the suction filtration system (6) corresponds to the rotary worktable (7), a feeding and discharging station (11), a suction filtration station (12), a first drying station (13) and a second drying station (14) are sequentially arranged on the upper circumference of the rotary worktable (7);

the control system is in signal connection with the mechanical arm (2), the mechanical claw (3), the scanning device (5), the suction filtration system (6), the rotary worktable (7) and the multifunctional post-processing device (8), and the control system can supply the working process to be automated through received signals.

2. A treatment device for proteins and metabolites in biological fluids according to claim 1, wherein: the pipette is used for extracting the sample body fluid in the sample test tube, and the grabber is used for grabbing the membrane adapter to move to a corresponding position.

3. A treatment device for proteins and metabolites in biological fluids according to claim 1, wherein: the scanning device (5) can record the information of the sample test tube, and the suction filtration system (6) supplies suction filtration of the membrane adapter.

4. A treatment device for proteins and metabolites in biological fluids according to claim 1, wherein: the multifunctional post-processing device (8) is used for spraying and printing information on the code-spraying packing paper, is placed in the membrane adapter and can also be used for separating the membrane adapter.

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