CN114720710A - Sample processing device with double-turntable structure - Google Patents

Abstract

The invention relates to the technical field of automatic sample processing equipment in the medical field, in particular to a sample processing device with a double-turntable structure, which comprises a base and a plurality of consumable receiving racks, wherein the base comprises a first turntable, a plurality of transfer liquid receiving units are arranged in the circumferential direction of the first turntable, a second turntable is arranged in the center of the first turntable, and N consumable receiving racks are arranged on the second turntable; the pipetting module is composed of at least one pipetting subunit, takes up and installs at least one consumable on a consumable receiving rack of the second turntable, and transfers the sample liquid or unloads the consumable in an area outside the second turntable; the beneficial effects are as follows: the invention realizes that the process of loading the Tip head and the process of pipetting or unloading the Tip head do not have spatial overlap, and avoids the cross contamination risk possibly caused by the overlap of the used Tip head motion path and a new loading path.

Description

A sample processing device with double turntable structure

technical field

The invention relates to the technical field of automatic sample processing equipment in the medical field, in particular to a sample processing device with a double turntable structure.

Background technique

In recent years, diseases caused by viruses and bacteria have attracted more and more attention. From a historical point of view, public security incidents with large-scale harm caused by viruses are also testing human beings, and also bring great impact on human production and life. It has created great challenges, brought suffering and even death to countless people, and also severely hindered the development of the world economy. In addition, with the pursuit of healthy life by human beings, more and more detection technologies based on the human body's own blood, cells, secretions and excreta have also developed rapidly. Congenital disease screening, disease screening and diagnosis and treatment plan rationality judgment plan based on whole blood samples, disease screening based on nasal swabs, throat swabs and anal swabs, etc.

Driven by more other requirements, the above two requirements require a sample processing system with faster speed, lower pollution risk, and less manual participation to meet the requirements. The sample processing system or related modules developed in the prior art include: The US invention patent US7985375B2 Sample preparation system and method for processing clinical specimens discloses a fully automatic consumable sample loading device including a pipette, a consumable sample loading part, a barcode identification part, etc. The design has a non-negligible risk of contamination due to aerosols during sample liquid handling. The European patent for invention EP3467511B1 Automaticanalyzer and operating method for same discloses a multi-sample analysis system based on the design of the double-turntable sample addition section. Although the sample processing speed of the analysis system can be improved, the sample tube addition of the double-turntable still has the risk of contamination . The solution disclosed in the US invention patent US10613106B2 Reaction vessel handlingapparatus, testing apparatus, and methods using the same is a spatially overlapping design for the sample tube and the transfer receiving part. This design requires two rotating drive systems with high matching precision, so To ensure the accurate transfer of the sample liquid in each sample tube distributed around the circumference, the technical complexity is higher and the risk of contamination in the system cannot be reduced. The pretreatment system disclosed in US patent application US20210293671A1 Devices and components for automated tissue processing and staining and uses thereof is based on the design of special consumables, and a segmented layer structure is designed to reduce contamination. However, this system cannot be used with general-purpose deep well plate consumables. Combined use results in limited promotion and high cost and complexity.

However, with the application of higher sensitivity reagents, very small contamination may also increase the probability of false positives in the final diagnosis process, and this risk is also an unacceptably significant risk. Therefore, it has become an urgent technical problem to reduce the risk of cross-contamination that may occur in the sample liquid processing system through the rational design of the layout structure, and at the same time to be compatible with the low-cost design requirements.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a sample processing device with a double turntable structure in view of the deficiencies in the prior art, which can reduce the risk of cross-contamination that may occur inside the sample liquid processing system through rational design of the layout structure, and can also Compatible with low-cost design requirements.

In order to achieve the above object, the technical scheme provided by the invention is:

A sample processing device with a double turntable structure is characterized by comprising a first turntable 10, a plurality of transfer liquid receiving units are arranged in the circumferential direction of the first turntable 10, and a first turntable 10 is arranged in the center of the first turntable 10. Two turntables 20, on which N consumables receiving racks 201 are arranged; and a pipetting module 30, the pipetting module 30 is composed of at least one pipetting sub-unit, and the pipetting module 30 is located in the At least one consumable is ingested and installed on a consumable receiving rack 201 of the second turntable 20 , and sample liquid transfer or consumable unloading is performed in an area outside the second turntable 20 .

Preferably, the transfer fluid receiving unit includes a receiving portion 101 , and a deep well plate is connected to the receiving portion 101 through a first clamping portion 1011 .

Preferably, the tip of the pipette is connected to the consumables receiving rack 201 through the second engaging portion 2011 .

Preferably, the first turntable 10 and the second turntable 20 have independent drive structures, so that the two turntables can rotate independently.

Preferably, the first turntable 10 is driven by the first motor through meshing gear pair transmission, and the second turntable 20 is driven by the second motor through pulley transmission.

Preferably, the meshing gear pair is a configuration in which a low number of teeth is matched with a high number of teeth.

Preferably, the high-tooth gear is supported by not less than two auxiliary support wheels.

Preferably, the first turntable and the second turntable are located on the same horizontal plane, and the central axes of the two turntables are substantially coincident.

Preferably, when the first turntable (10) completes one rotation, the second turntable (20) rotates no less than 1/N turn.

Preferably, the number of the transfer liquid receiving units is 6, and the number N of the consumables receiving racks (201) is 2.

Compared with the prior art, the present invention has the following advantages:

1. In the solution of the present invention, a plurality of sample liquid receiving units are arranged in the circumferential direction of the outer ring turntable, the inner ring turntable is set in the center of the outer ring, and N consumables receiving racks are provided, and the consumables can be used by pipettes for pipetting. Tip head, a pipetting part consisting of not less than one pipetting subunit, the pipetting part ingests and installs at least one pipetting tip consumable on the consumable receiving rack in one of the central positions, and installs it in the In the area outside the center of the first turntable, operations such as sample liquid transfer or unloading of consumables are performed, so that there is no spatial overlap between the process of loading the tip and the process of pipetting or unloading the tip, and the movement of the tip that has been used is avoided. There is a risk of cross-contamination that can result from overlapping paths and new load paths.

2. The sample liquid receiving unit of the present invention is set as deep-well plate consumables (for example, a deep-well plate with more 96-well plates and 16 sample receiving wells), which ensures the compatibility of the pre-processing system and can be matched with different nucleic acid extractions For example, the GeneRotex Nucleic Acid Extractor of Tianlong Technology is used, and the number of consumable receiving racks in the center can meet the needs of larger sample processing.

3. The present invention achieves the satisfaction of different rotation requirements of the inner and outer rings through independent driving sources such as independent motor drives, and through the cooperative use of gear pairs and pulley drives, the entire double turntable system can operate reliably without interfering with each other. The combination with the low number of teeth can be compatible with more transfer fluid receiving units, and can also adapt to the position adjustment of smaller angles. With the auxiliary support of no less than two auxiliary support wheels, the entire transmission system can run smoothly and reliably. The two turntables are basically The two turntables are located on the same horizontal plane, and the central axes of the two turntables are basically coincident. This design ensures that there is no excessive operation error in operations such as transfer and picking of the pipette, and ensures the reliability of the entire system.

4. In the present invention, after the double turntable completes a full circle of rotation according to the outer ring turntable, the inner ring turntable rotates no less than 1/N circle, thus realizing the relative immobilization of the pipette installation consumables and the replacement and installation of new consumables , which also ensures the continuity of the installation and unloading of the sample liquid receiving unit, and realizes the compatibility of different types of deep well plates from different companies with the pipetting sub-units with variable spacing.

Description of drawings

For a clearer understanding of the present invention, the present disclosure will be further introduced by combining the accompanying drawings and schematic embodiments of the description. The accompanying drawings and embodiments are used for explanation and do not constitute a limitation on the disclosure.

Fig. 1 is a kind of double turntable layout structure schematic diagram provided by the present invention;

2 is a schematic cross-sectional view of a double-turntable layout structure provided by the present invention;

Fig. 3 is a kind of double turntable layout provided by the present invention with auxiliary wheel structure schematic diagram;

4 is a schematic diagram of the movement trajectory of the pipette under a double turntable structure provided by the present invention;

5 is a schematic diagram 1 of a processing system including a double turntable structure provided by the present invention;

6 is a schematic diagram 2 of a processing system comprising a double turntable structure provided by the present invention;

7 is a schematic diagram 3 of a processing system including a double turntable structure provided by the present invention;

8 is a schematic diagram of functional modules of a sample processing system with a double turntable structure provided by the present invention;

FIG. 9 is a schematic diagram of the operation flow of a sample processing system provided by the present invention.

Shown in the figure: the first turntable 10, the receiving part 101, the first engaging part 1011, the second turntable 20, the second engaging part 2011 of the consumables receiving frame 201, the first driving motor 11, the output gear 112, the driving gear 113 , the connecting piece 114, the second drive motor 21, the output pulley 211, the belt 212, the drive pulley 213, the shaft 214, the first auxiliary support wheel 1131, and the second auxiliary support wheel 1132.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments It is a part of the embodiments of the present application, but not all of the embodiments. The components of the embodiments of the present application generally described and illustrated in the drawings herein may be arranged and designed in a variety of different configurations.

Thus, the following detailed description of the embodiments of the application provided in the accompanying drawings is not intended to limit the scope of the application as claimed, but is merely representative of selected embodiments of the application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

It should be noted that like numerals and letters refer to like items in the following figures, so once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

Example 1

1 is a schematic structural diagram of a double turntable provided by an embodiment of the present invention, which includes a plurality of transfer fluid receiving units arranged in the circumferential direction of the first turntable 10. Currently, human secretions, excrement, blood, amniotic fluid, nasal swabs are used , throat swabs, anal swabs, etc. as the object of the in vitro diagnostic technology has become an early and rapid diagnosis scheme for detecting the infection of a certain disease. It plays an increasingly important role in public security, medical diagnosis, crime detection, etc. The transfer fluid receiving unit in the system of Example 1 is the deep-well plate most used in molecular diagnosis, and the sample processing system can be used to automatically establish The nucleic acid extraction reaction system is used, thereby greatly reducing the amount of manual operations and ensuring the reliability of subsequent test results. The deep well plates here can be of different volume types, such as sample processing volumes of 1 mL, 1.5 mL, 2 mL, 2.5 mL, 3 mL, and so on. Of course, the dual-turntable sample processing system including this embodiment can be used as an independent product, and then used in combination with nucleic acid extraction products to complete a fully automated extraction process. The use of nucleic acid extraction equipment is not limited here. It can also be used as a sub-module of automated analysis equipment to complete a fully automatic solution from sample addition to final result output, which is not limited here. Here, the first turntable 10 includes a plurality of receiving parts 101, which can receive 5, 6, 7, 8, 9, etc. deep-well plates. The deep-well plate is the most widely used structure of 96 wells and 16 sample positions. Compatible with deep well plates of different capacities, the plurality of receiving parts 101 of the first turntable 10 all include elastic snap-on parts 1011, which can be arranged in pairs, using the adjustable characteristics of the elastic parts to form openings of different sizes so as to accept different manufacturers Or different types of deep well plates. A second turntable 20 is also provided in the center of the first turntable, and the second turntable includes N consumables receiving racks 201 (wherein N is an integer not less than 1). In use, N can also be the number of 3, 4, 5, etc., which is not limited here. The consumables here are single-use items, the most typical of which is the tip of a pipette. The most commonly used commercial tip is usually made into a box of 96 batches. In order to be compatible with the 96pcs batch commercial mode, The second turntable 20 uses the engaging portion 2011 to ensure the installation of N corresponding numbers of pipetting tips. In order to ensure the establishment of the reliability of the extraction system, a PK&IC liquid storage portion 110 is set on the periphery of the first turntable 10, so as to meet the requirements of removing DNA and RNA. The nuclease and buffer in the preparation ensure that the reaction conditions of the reaction solution do not change too quickly, etc. The specific structure is not limited here.

Example 2

2 is a schematic cross-sectional view of the double turntable structure provided by the present invention. The rotation of the double turntable is driven by two independent motors, so that two different rotation angles, different rotation timing arrangements and even different rotation directions can be realized. The first drive motor 11 is connected to the output gear 112 through the output shaft, thereby driving the output gear 112 to rotate. The output gear 112 is engaged with the drive gear 113 of the first turntable 10 , and the drive gear 113 can pass through no less than one connecting piece 114 Directly connected with the first turntable 10 , the two can realize rotational motion without relative motion, thereby completing the conversion of the rotation transmission of the first drive motor 11 into the rotation of the first turntable 10 . In order to ensure that the rotation of the first turntable 10 has the most precise adjustment angle, the driving gear 113 and the output gear 112 are designed with a high gear ratio. Of course, in one case, the first drive motor 11 may continuously rotate at a predetermined angle each time, such as 60°, 40°, etc. each time, and then pause for a specific time to reserve the time required for pipetting operations, etc., Then continue to rotate, so as to realize the high-precision continuous operation in which the operation hole position of the pipette is basically fixed, and the upper and lower sample positions are basically fixed. The second drive motor 21 is connected to the output pulley 211 through the output shaft, and is connected to the drive pulley 213 of the second turntable 20 at the center position through the belt 212. The drive pulley 213 drives the shaft 214 directly connected to the second turntable 20 to rotate, thereby The rotation drive of the second turntable 20 in the center position is realized. The present invention drives the two turntables by using two different transmission modes. The two turntables can run the same clockwise or counterclockwise, or one can run clockwise and the other It does the opposite counterclockwise operation, which can also meet the requirements of different precision control and adapt to the fast and low-cost requirements of the system. The first turntable 10 and the second turntable 20 are basically located on the same horizontal plane, and the central axes of the two turntables are basically coincident, which ensures the simplification of the control of the pipette matched with the double turntable, and ensures that the system low-cost and low-complexity implementation.

Example 3

FIG. 3 is a schematic diagram of the partial optimization design of the gear transmission provided by the present invention. In order to ensure the precise angle control of the first turntable, the driving gear 113 needs to have a relatively high number of teeth, so it is necessary to ensure that the driving gear 113 has a sufficient radius, so that in the long-term working process Due to the radial force and other effects, the gear meshing may be unreliable or even fail, so the high-tooth driving gear 113 is supported by not less than two auxiliary support wheels 1131 and 1132, so as to ensure that the entire equipment can be used during the service life. The reliable operation of the system is ensured, and both the meshing accuracy and the operation reliability are realized at a low cost. Combined with Figure 1, multiple transfer liquid receiving units can be optimally set to six deep-well plate receiving parts, so that a one-time processing of 96 samples can be completed when the deep-well plate is fully loaded, and the second turntable in the center contains consumables. The number N of receiving racks is 2, so that when all the deep-well plates on the outer ring turntable 10 correctly receive the sample liquid corresponding to the hole position, the first turntable 10 completes one rotation, and one of the corresponding consumables receiving racks is The tip consumables are also used up. At this time, the second turntable 20 can rotate 1/2 turn, and the supply of new consumables rotates to the original position, which realizes the complicated control of the pick-up trajectory of the pipette and can start a new one without readjusting. For pipetting operations, new consumables can also be reloaded by using the completed consumables receiving rack. When N is other values, when the first turntable 10 completes one rotation of the rotation, the second turntable 20 rotates not less than 1/N This ensures the continuity of the entire operation process and the rationality of the entire configuration.

Example 4

4 is a schematic diagram of the movement trajectory of the pipette under a double turntable structure provided by the present invention. Two turntables 20, which include N consumables receiving racks, 30 is a pipetting module, which can include 2, 3, 4, etc. pipetting sub-units for sample liquid transfer, 401 is a shaking and mixing sub-unit, which can For mixing and transporting the sample liquid, in the actual pipetting process, firstly, the pipette enters the inner ring area of the second turntable 20 along the first track of S10. At this time, the pipette does not carry any used Tip head, thus avoiding the risk of contamination caused by bringing the used Tip head into the new consumable area, and then taking the new pipetting Tip head through the drive of the motion motor, and the pipette crosses the top area of the pipetting receiving part after the intake is completed. , at this time, since it is an unused tip, it will not affect the pipetting receiving part. Then it sucks the sample liquid after the oscillation along the paths of S201 and S202 and transfers it to the corresponding hole of the sample liquid receiving part to complete the transfer. Finally, the pipette takes the used Tip head and moves directly to the consumables recovery hole along the S30 path, and unloads the used Tip head consumables for centralized recycling. This cycle can complete all the transfer operations of the sample solution with low risk of contamination. Under this layout, the trajectories of the new tip and the used tip have almost no overlap, which minimizes the risk of cross-contamination. In addition, the use is completed. The movement trajectory of the tip consumables does not involve the central area where the second turntable 20 is located, which also minimizes the risk of cross-contamination between the old and new tips. The tip consumables area can maintain a fixed low pollution risk area. The middle pipetting track can also be the combined S20 track, which is not limited here. Of course, after the transfer of samples in all the well positions of a sample liquid receiving part is completed, the first turntable 10 can be rotated by a predetermined angle, so that the new sample liquid receiving part that does not receive any sample liquid is rotated to the previous hole position, thus realizing the movement of the pipette The simplification of trajectory control also ensures the accuracy of pipetting operations. Of course, the second turntable 20 inside can also adopt a similar design, so that the second turntable 20 rotates at another preset angle after the consumables on one of the sample racks are used. , to realize the switching of consumable racks, which is not limited here.

Example 5

FIG. 5 is a sample liquid pre-processing system including the double-turntable structure of the present invention, which includes a sample addition and loading unit 60 for sample tubes, which can be added to sample tubes such as 96, 192, 288, 384, etc., for one-time processing. . The sample tube transfer gripper 50 is used for grabbing or unloading into the sample tube from the sample tube receiving portion of the sample loading unit 60. In order to ensure that the transferred sample liquid can contain enough objects to be detected, it is usually necessary to properly carry out the sample liquid. In the prior art, there are many automated systems that directly integrate the design of the shaking device on the sample loading unit to meet the needs of sample shaking and mixing. However, such a design requires simultaneous shaking of all sample tubes, and in batches In the chemical processing system, the sample tube volume of the sample loading unit 60 is large, and it is impossible to realize all the one-time simultaneous transfer design. Therefore, the direct design of the ensemble type of oscillation system will lead to a high design cost of the entire system. In order to maintain the turbulence degree. The requirements also have higher requirements on the reliability of the system operation, so the present invention does not adopt the design scheme that the oscillating units are assembled in the sample loading unit 60 . The sample liquid pre-processing system can include two or more sample shaking and mixing sub-modules 401, and the transfer gripper 50 includes the Z-axis up and down running track 511 and the Y-axis forward and backward running track 512, and of course, it can also include the left and right running track of the X axis. The track, which is not limited here, can achieve the effect that any position in the sample liquid processing system can be covered. The transfer gripper 50 can grab the sample tube in the sample loading unit 60 to the shaking and mixing subunit, and the shaking and mixing subunit can mix the sample liquid according to a predetermined degree of turbulence. Of course, the transfer gripper 50 can also be completed from Returning the sample liquid pipetting to the shaking and mixing sub-unit grabs the sample tube, so that the sample tube after pipetting is returned to the corresponding hole position before the sample loading unit 60 .

Example 6

With reference to Fig. 7, the shaking and mixing sub-unit completes the shaking and mixing, and is transported to the bottom of the switch cover unit 100. The shaking and mixing process can be performed simultaneously during the transfer movement of the sample tube, or a specific sequence can be directly arranged to complete the shaking and mixing process. . The switch cover unit 100 includes two switch cover units 1001 and 1002 corresponding to the number of sample tubes in the oscillator subunit, which include the switch cover gripper and the tube body fixing gripper matched with the tube body. Of course, it can also be used in the oscillator. The solution of setting a card and a fixing part in the unit to cooperate with the opening and closing of the lid clamping jaw is not limited to adopting any one of the two methods to open the sample tube. Of course, the two opening and closing lid units 1001 and 1002 can be completed at the same time. The cap opening operation of the two sample tubes can also be performed separately after one sample tube is opened, and then the other sample tube is opened after the pipette suction operation is completed. After the sample tube is opened, the pipette 30 can perform the pipetting operation. Before that, the pipette can complete the operation of picking up the Tip consumables at the second turntable 20 in the center of the double turntable structure. To transfer the sample liquid, the pipette 30 includes two sub-pipetting units 301 and 302. Further, in order to ensure that the transfer liquid can better adapt to the size characteristics of the pipetting receiving part, the distance between the two pipetting sub-units can be adjusted, and the two pipetting sub-units can be adjusted. The distance between the two can be 20mm-70mm. The pipette 30 also includes X, Y, and Z axis motion drive mechanisms. In order to ensure the simplicity of the system design, cost reduction and reliability requirements, the pipette and the sample tube The X-axis of the transfer fixture shares the motion track. The pipette 30 can cooperate with the Tip head to suck the sample liquid in the sample tube at the same time or separately, and then move along the non-central area where the second turntable 20 is located to above the corresponding hole position of the deep-well plate that receives the sample liquid, and then simultaneously or separately. Remove the transferred sample solution to the corresponding well of the deep-well plate to complete the transfer. After the sample tube is used, the cover is re-covered by the switch cover module, and the sample tube is transported and unloaded by the shaking and mixing sub-unit. Move the trajectory that will not infect the central area to the unloading hole position to unload the tip. Of course, before pipetting, the pipette can first transfer an appropriate amount of PK&IC liquid from the PK&IC storage unit 110 to the corresponding hole position, so as to realize the reliability of the extraction system. Therefore, since the liquid is a relatively general reagent, it will not be used for subsequent There is an impact on sample liquid transfer, which ensures the simplicity of the pipetting system design.

Example 7

6 is a sample liquid processing system from another perspective, which includes a sample information acquisition unit 70, which can identify barcodes, two-dimensional codes, FRIDs, etc. of the sample tubes. For example, it can cooperate with the opening and closing device in FIG. 7 to obtain For identification and acquisition of sample information, the cover opening and closing device can grab the sample tube and make the sample tube within the field of view of the information acquisition unit 70, and drive the rotation of the sample tube through the opening and closing cover to identify the corresponding information of the sample. Of course, other methods can also be used. The acquisition of sample information after the solution is completed is not limited here. After the pipette completes pipetting, the used consumable tip is unloaded at the consumables receiving hole 801, and the tip consumables recovery position is set at the front of the pre-processing system, in which A drawer-like design can be used to recycle the discarded Tip heads for subsequent centralized treatment, which also ensures low pollution risk of the system. The pre-treatment system also includes an ultraviolet sterilization unit 90 to ensure the cleanliness of the operating environment during the treatment process. With the basic fully automated operation, the contamination risk of the entire sample processing process is eliminated to the greatest extent, and it can also be set in the Tip recovery drawer to sterilize the used Tip heads.

Example 8

8 is a schematic diagram of the functional modules of a sample processing system with a double turntable structure provided by the present invention, which can be divided into four basic sub-functional units, including the sample adding unit U01 functional module, which is mainly responsible for adding samples and whether the sample is in place, etc. It includes the sample input unit 60 (also referred to as the sample loading unit) receiving batch input sample tubes and sample racks, which can contain several position sensors located at different positions, which can be of photoelectric or mechanical type. The location is not limited. When the detection is in place, the sample adding unit U01 is ready. The transfer unit U02 function module is mainly responsible for the transfer operation of the sample tube. This part is responsible for the bridge function before pipetting. It includes the sample tube clamp 50 for transferring a new sample tube into the oscillator subunit 40, or from the oscillator subunit. 40, remove the sample tube that has been pipetted, and the vibrating subunit can have both the functions of conveying and vibrating and mixing. The function module of the pipetting unit U03 is mainly used to complete the transfer of the sample liquid after the identification and acquisition of the sample liquid information. It includes a code scanning sub-unit 70, which can obtain the sample information on the one hand, and verify whether the system has the correct reagents on the other hand. For receiving the sample liquid, the switch cover unit 100 also includes two basic functions, 1) opening or closing the cover of the sample tube; 2) the auxiliary scanning sub-unit 70 to obtain the sample tube information, and the pipetting module 30 may include multiple sub-pipettes The suction of the sample liquid and the discharge to the target hole are performed. The system establishment unit U04 is a functional module, in which the system establishment of subsequent operations is completed, for example, the extraction system establishment in this system, which includes a double turntable subunit, covering the deep well plate bearing turntable and the first turntable 10 in the outer ring. The consumable rack receiving part of the second turntable 20 in the center inner ring, and other trace reagent storage subunits 110 that need to be added during the reaction process can be the PK&IC solution required for the extraction process in this embodiment, which is not limited here.

Example 9

FIG. 9 is a schematic diagram of the operation flow of a sample processing system provided by the present invention. As the system is turned on, the operation program of the entire sample processing system starts to be executed, and the sample loading unit receives the sample tubes added manually or transferred from the refrigerating part by the automated robotic arm. There are several sensors inside the sample loading unit to detect whether the sample tube rack is loaded in place and whether it is loaded correctly. After the sample tube is loaded correctly, the sample tube is clamped by the sample tube clamp and transferred to the receiving part. The oscillator sub-unit is fully mixed, and then the cover opening mechanism can cooperate with the sample tube identification unit to identify and obtain the sample tube information. On the other hand, verify the barcode information of the sample tube, including but not limited to the verification of the sample test item, whether the relevant reagents of the system experiment are correct, and whether the sample liquid is collected correctly, etc. After the verification is completed, if the sample tubes are matched correctly and the system reagents are sufficient, the next steps can be performed. The established database information can also be transmitted to other equipment used in conjunction with the local area network. If not, a warning message will be generated. In some cases, the system It can continue to run and only generate an alarm signal, and generate an alarm signal under some conditions that cannot be solved by the system itself. At this time, the operator can perform intervention processing to solve the problem. Of course, the above-mentioned scanning code identification step can be arranged before the sample liquid shaking and mixing step. After completing the information acquisition and verification, the cap opening and closing unit performs the cap opening operation, during or before the pipette picks up the Tip consumable at the center of the inner ring, and transfers the sample liquid along the pipetting path in the cap opening state. Step, transfer the sample liquid to the sample liquid receiving part, which can be the corresponding hole position of the deep-well plate. After the transfer, the sample tube is closed, and the shaking and mixing unit transports it to the unloading position, and then is transferred back to the initial position. , after the transfer of the sample liquid is completed, the pipette runs to the recovery hole of the Tip head consumables, and the used Tip head consumables are unloaded to complete the transfer of the sample liquid. Of course, the double turntable structure of the present invention can also be used in a fully automated sample analysis system to complete the processing and analysis of the sample liquid and the final result output under the condition of low contamination risk, which is not limited here.

Those skilled in the art should understand that the present invention is not limited by the above-mentioned embodiments, and the descriptions in the above-mentioned embodiments and the description are only to illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have Various changes and modifications fall within the scope of the claimed invention. The claimed scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

1. The utility model provides a take sample processing apparatus of two carousel structures which characterized in that: the transfer liquid feeding device comprises a first rotary table (10), wherein a plurality of transfer liquid receiving units are arranged in the circumferential direction of the first rotary table (10), a second rotary table (20) is arranged at the center of the first rotary table (10), and N consumable receiving racks (201) are arranged on the second rotary table (20); the liquid transfer device further comprises a liquid transfer module (30), the liquid transfer module (30) comprises at least one liquid transfer subunit, the liquid transfer module (30) is used for mounting at least one consumable on a consumable receiving rack (201) of the second turntable (20) in an uptake mode, and sample liquid transfer or consumable unloading is carried out in an area outside the second turntable (20).

2. The sample processing device with a dual turntable structure of claim 1, wherein: the transfer liquid receiving unit comprises a receiving part (101), and a deep hole plate is connected to the receiving part (101) through a first clamping part (1011).

3. The sample processing device with a dual turntable structure of claim 1, wherein: the consumable receiving frame (201) is connected with a Tip head of a liquid transfer device through a second clamping portion (2011).

4. The sample processing device with a dual turntable structure of claim 1, wherein: the first turntable (10) and the second turntable (20) are provided with independent driving structures, so that the two turntables can rotate independently.

5. The sample processing device with a dual turntable structure of claim 4, wherein: the first rotary table (10) is driven by a first motor through meshing gear pair transmission, and the second rotary table (20) is driven by a second motor through belt wheel transmission.

6. The sample processing device with a dual turntable structure of claim 5, wherein: the meshing gear pair is configured with a low tooth number matched with a high tooth number.

7. The sample processing device with a dual turntable structure of claim 6, wherein: the high-tooth-number gear is supported by at least two auxiliary supporting wheels.

8. The sample processing device with dual turntable structure of claim 1, wherein: the first rotating disc and the second rotating disc are positioned on the same horizontal plane, and the central axes of the two rotating discs are basically coincident.

9. The sample processing device with a dual turntable structure of claim 4, wherein: when the first rotary table (10) completes one circle of rotation, the second rotary table (20) rotates for no less than 1/N circle.

10. A sample processing device with a dual turntable structure as claimed in claim 1, wherein: the number of the transfer liquid receiving units is 6, and the number N of the consumable receiving racks (201) is 2.

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