CN217438147U - Manual extraction element of DNA - Google Patents

Abstract

The utility model discloses a DNA manual extraction device, including fixed film, deep hole board and magnetic force board, the deep hole board sets up the fixed film top, the magnetic force board sets up the deep hole board top, the magnetic force board with the deep hole board phase-match, deep hole has been seted up to the deep hole board inside, deep hole board bottom is seted up flutedly, the recess is inside fixedly connected with deep hole base, deep hole base is located the deep hole bottom, and the deep hole base is linked together with the deep hole, fixed film inside has seted up the draw-in groove, the fixed film inside fixedly connected with boss, boss and the draw-in groove with the deep hole board bottom recess phase-match, the deep hole base is flat bottom structure; the utility model provides an among the technical scheme, through combining fixed film deep hole board and magnetic plate, can need not to purchase expensive machine, and it is higher than adsorption column extraction method flux, the time is shorter, and the DNA solution of drawing the completion can be preserved in the orifice plate for a long time.

Description

Manual extraction element of DNA

Technical Field

The utility model relates to the technical field of medical appliances, in particular to a DNA manual extraction device.

Background

The extraction of DNA is mainly a CTAB method, and other methods include physical methods such as a glass bead method, an ultrasonic method, a grinding method, and a freeze-thawing method. Chemical methods such as guanidinium isothiocyanate method and alkaline lysis method. The biological mode comprises the following steps: an enzymatic method. According to the different nucleic acid separation and purification methods, there are siliceous materials, anion exchange resins, etc., and when DNA is extracted, an automatic DNA extraction apparatus or an adsorption column extraction method is required.

The prior art has the following defects or problems:

at present, most of DNA extraction instruments or adsorption columns are adopted for extracting DNA, the DNA automatic extraction instruments are expensive and high in cost, and the adsorption columns are low in flux, so that the DNA extraction efficiency is low, and time and labor are wasted.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a to prior art's weak point, provide the manual extraction element of DNA to solve the problem that provides in the background art.

In order to achieve the above object, the utility model provides a following technical scheme:

the manual DNA extraction device comprises a fixed film, a deep hole plate and a magnetic plate, wherein the deep hole plate is arranged at the top of the fixed film, the magnetic plate is arranged at the top of the deep hole plate, and the magnetic plate is matched with the deep hole plate.

Optionally, a deep hole is formed in the deep hole plate, a groove is formed in the bottom of the deep hole plate, a deep hole base is fixedly connected to the inside of the groove, the deep hole base is located at the bottom of the deep hole, and the deep hole base is communicated with the deep hole.

Optionally, a clamping groove is formed in the fixed film, a boss is fixedly connected in the fixed film, and the boss is matched with the clamping groove and the groove in the bottom of the deep hole plate.

Optionally, a fixing hole matched with the deep hole base is formed in the boss, and the deep hole plate is fixedly mounted on the top of the fixing film through the fixing hole and the clamping groove.

Optionally, the deep-hole base is of a flat-bottom structure.

Optionally, the bottom of the magnetic plate is fixedly connected with a magnetic column, the magnetic column is matched with the deep hole, and the magnetic plate is fixedly mounted on the top of the deep hole plate through the magnetic column.

Optionally, the fixing film is made of silica gel.

Compared with the prior art, the utility model provides a manual extraction element of DNA possesses following beneficial effect:

1. the utility model combines the fixed film deep hole plate and the magnetic plate, so that expensive machines do not need to be purchased, the flux is higher and the time is shorter than that of an adsorption column extraction method, and the extracted DNA solution can be stored in the hole plate for a long time;

2. the utility model discloses an establish deep hole base bottom to flat structure can make the inside magnetic column of magnetic plate better with the laminating of deep hole bottom.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the fixing film of the present invention;

FIG. 3 is a schematic diagram of the deep-hole plate structure of the present invention;

FIG. 4 is a schematic view of the bottom structure of the deep hole plate of the present invention;

fig. 5 is a schematic diagram of the structure of the magnetic plate of the present invention.

In the figure: 1. fixing the film; 2. a deep hole plate; 3. a magnetic plate; 4. a card slot; 5. a boss; 6. a fixing hole; 7. deep holes; 8. a deep hole base; 9. a magnetic column.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should be further noted that, unless explicitly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected, mechanically connected, electrically connected, directly connected, connected through an intermediate medium, or connected to the inside of two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Referring to fig. 1-5, in this embodiment: the DNA manual extraction device comprises a fixed film 1, a deep hole plate 2 and a magnetic plate 3, wherein the deep hole plate 2 is arranged at the top of the fixed film 1, the magnetic plate 3 is arranged at the top of the deep hole plate 2, and the magnetic plate 3 is matched with the deep hole plate 2; by combining the fixed film 1, the deep-hole plate 2 and the magnetic plate 3, expensive machines are not required to be purchased, the flux is higher and the time is shorter than that of an adsorption column extraction method, and the extracted DNA solution can be stored in the hole plate for a long time.

A deep hole 7 is formed in the deep hole plate 2, a groove is formed in the bottom of the deep hole plate 2, a deep hole base 8 is fixedly connected in the groove, the deep hole base 8 is located at the bottom of the deep hole 7, and the deep hole base 8 is communicated with the deep hole 7; the deep hole 7 in the deep hole plate 2 can be provided with 24, 48 or 96, preferably 96, deep holes 7 with the capacity of 1.2 ml-2.2 ml, wherein the preferred flux is 2.2 ml.

A clamping groove 4 is formed in the fixed film 1, a boss 5 is fixedly connected in the fixed film 1, and the boss 5 and the clamping groove 4 are matched with a groove at the bottom of the deep hole plate 2; through set up draw-in groove 4 in fixed membrane 1 is inside, can carry out the joint to deep-hole board 2 through draw-in groove 4.

A fixing hole 6 matched with the deep hole base 8 is formed in the boss 5, and the deep hole plate 2 is fixedly arranged on the top of the fixed film 1 through the fixing hole 6 and the clamping groove 4; the boss 5 may be provided with 24, 48 or 96 internal fixation holes 6, preferably 96.

The deep hole base 8 is of a flat bottom structure; the bottom of the deep hole base 8 is designed to be a flat bottom structure, so that the magnetic column 9 in the magnetic plate 3 can be better attached to the bottom of the deep hole 7.

The bottom of the magnetic plate 3 is fixedly connected with a magnetic column 9, the magnetic column 9 is matched with the deep hole 7, and the magnetic plate 3 is fixedly arranged on the top of the deep hole plate 2 through the magnetic column 9; the number of the magnetic columns 9 at the bottom of the magnetic plate 3 can be 24, 48 or 96, and the preferred number is 96.

The fixed membrane 1 is made of silica gel; the fixed membrane 1 is made of silica gel, so that the fixed membrane 1 has stronger applicability and certain anti-skid effect.

The utility model discloses a theory of operation and use flow: the deep hole plate 2 is fixed on the top of the fixed film 1 through a clamping groove 4 in the fixed film 1 and a fixing hole 6 in the boss 5, and the fixed film 1 can prevent liquid leakage and pollution among holes; put into deep hole 7 with DNA solution, every deep hole 7 both is the space of carrying out the DNA and drawing in deep hole board 2, installs magnetic plate 3 at deep hole board 2 top again, makes magnetic column 9 be located deep hole 7 inside, sets up deep hole base 8 bottom to the flat structure and can make the magnetic column 9 of magnetic plate 3 inside better with deep hole 7 bottom laminating, adsorbs downthehole magnetic bead through the magnetic force of magnetic column 9, accomplishes operations such as absorption, washing and elution of DNA.

The above only is the preferred embodiment of the present invention, not so limiting the patent scope of the present invention, all under the concept of the present invention, the equivalent structure transformation made by the contents of the specification and the drawings is utilized, or the direct/indirect application is included in other related technical fields in the patent protection scope of the present invention.

Claims (7)

1. The manual extraction device of DNA, its characterized in that: including fixed film (1), deep hole board (2) and magnetic plate (3), deep hole board (2) set up fixed film (1) top, magnetic plate (3) set up deep hole board (2) top, magnetic plate (3) with deep hole board (2) phase-match.
2. 2. The manual DNA extraction apparatus according to claim 1, characterized in that: deep hole (7) have been seted up to deep hole board (2) inside, the recess has been seted up to deep hole board (2) bottom, the inside fixedly connected with deep hole base (8) of recess, deep hole base (8) are located deep hole (7) bottom, just deep hole base (8) with deep hole (7) are linked together.
3. 3. The manual DNA extraction device according to claim 2, characterized in that: the fixing film is characterized in that a clamping groove (4) is formed in the fixing film (1), a boss (5) is fixedly connected to the inside of the fixing film (1), and the boss (5) is matched with the clamping groove (4) and a groove in the bottom of the deep hole plate (2).
4. 4. The manual DNA extraction apparatus according to claim 3, characterized in that: the deep hole plate is characterized in that a fixing hole (6) matched with the deep hole base (8) is formed in the boss (5), and the deep hole plate (2) is fixedly installed at the top of the fixed film (1) through the fixing hole (6) and the clamping groove (4).
5. 5. The manual DNA extraction apparatus according to claim 2, characterized in that: the deep hole base (8) is of a flat bottom structure.
6. 6. The manual DNA extraction apparatus according to claim 2, characterized in that: the bottom of the magnetic plate (3) is fixedly connected with a magnetic column (9), the magnetic column (9) is matched with the deep hole (7), and the magnetic plate (3) is fixedly installed at the top of the deep hole plate (2) through the magnetic column (9).
7. 7. The manual DNA extraction apparatus according to claim 1, characterized in that: the fixed film (1) is made of silica gel.

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