CN217297855U - Single cell parcel appearance - Google Patents
Single cell parcel appearance Download PDFInfo
- Publication number
- CN217297855U CN217297855U CN202123283480.1U CN202123283480U CN217297855U CN 217297855 U CN217297855 U CN 217297855U CN 202123283480 U CN202123283480 U CN 202123283480U CN 217297855 U CN217297855 U CN 217297855U
- Authority
- CN
- China
- Prior art keywords
- chip
- fluid input
- module
- air pressure
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
- Y02P70/50—Manufacturing or production processes characterised by the final manufactured product
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model provides a single cell packaging instrument, which comprises a micro-fluidic chip, a chip placing module, a circuit module and an air circuit module, wherein the micro-fluidic chip comprises a plurality of sets of liquid drop generators and can be used for generating single cell multi-particle liquid drops at high flux; the micro-fluidic chip is hermetically butted with the gas circuit module and the circuit module through the chip placement module, and each fluid shares one electric proportional valve, so that the liquid drops can be produced automatically at high flux, and the efficiency and the energy can be improved; therefore, the device realizes the high-flux, high-efficiency and high-automation generation of the unicellular multiparticulate liquid drop, and has the advantages of simple structure, convenience, high efficiency, low cost and easy popularization.
Description
Technical Field
The utility model relates to a micro-fluidic system field particularly, relates to a unicellular parcel appearance for generating many granule liquid drops.
Background
Because the Microfluidic Chip can integrate basic operation units related to the fields of chemistry, biology and the like, such as sample preparation, reaction, separation, detection, cell culture, sorting, lysis and the like, and different functions can be realized on the Microfluidic Chip by designing flow channels with different shapes, the Microfluidic Chip is also called a Lab-on-a-Chip, compared with the traditional laboratory, the Microfluidic Chip has the advantages of less reagent consumption, short reaction or analysis time and the like, the consumption of expensive reagents is reduced, and the cost can be controlled. The shortening of the time is beneficial to shortening the experimental period, and the experimental cost is greatly reduced in time and space by combining the chip size of square centimeter level or even square millimeter. The micro-fluidic application fields are many, and the micro-fluidic chip has important application in various fields such as chemistry, biology, medicine and the like.
The single cell sequencing is to sequence a single cell genome or transcriptome and the like to obtain genome, transcriptome or other multiomic information so as to reveal cell population differences and cell evolutionary relationships. The single cell sequencing technology can be used for detecting the heterogeneity among single cells, distinguishing a small number of cells and describing cell maps, and has great significance for biological research. Therefore, single cell sequencing technology has wide application in the fields of tumor, microbiology, neurology, reproduction, immunity, digestion, urinary, and the like. Among various single cell library preparation platforms, the droplet-type single cell library preparation platform has become the current mainstream application platform due to the advantages of methodology compatibility and stability, and is the most representative of the 10 × Genomics chromosome system.
The existing liquid drop type single cell library preparation platform is to wrap cells, transcription reagents and marked coding microspheres in water-in-oil droplets through a microfluidic chip to prepare single cell multi-particle droplets, thereby carrying out library construction coding and reverse transcription. By single-cell multiparticulate droplets is meant that each droplet generated contains more than two particles, including single cells, encoded microspheres, and the like.
The existing single-cell multi-particle liquid drops are usually generated through a micro-fluidic chip, but at present, an automatic device capable of being matched with the micro-fluidic chip is still lacked, even some single-cell multi-particle liquid drops are prepared by manually connecting the micro-fluidic chip with an air pressure device, the automation level is not high, the flux is low, the production efficiency is low, and operations such as incubation and the like for wrapping cells in the liquid drops cannot be realized. Therefore, an apparatus capable of performing automatic and high-throughput generation of single-cell multi-particle droplets is urgently needed, efficient generation of droplets is realized, and operations such as incubation after cells are wrapped in the droplets can be realized.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model provides a unicellular parcel appearance, place module, circuit module and circuit module including micro-fluidic chip, through designing the micro-fluidic chip of high flux to can place the module through the chip and realize the sealed butt joint of micro-fluidic chip and gas circuit module, thereby realize the high flux of many granule liquid drops of unicellular, high efficiency and high automatic generation, simple structure, convenient durable, with low costs, be suitable for the industrial production.
The single-cell multi-particle liquid drop of the utility model refers to that each prepared liquid drop contains a single cell and other particles. In single cell sequencing, a double particle liquid drop is used, and the double particle liquid drop contains 1 single cell and 1 coding microsphere with primer simultaneously, so that the single cell sequencing can be completed in the liquid drop reaction chamber.
The utility model provides a single cell packaging instrument, which comprises a chip, a chip placing module, a circuit module and an air circuit module, wherein a liquid drop generator is arranged in the chip, and a fluid input groove is arranged on the chip; the chip placing module is used for placing chips in a sealing mode, the gas circuit module is used for providing gas pressure for the fluid input grooves in the chips, and the circuit module is used for controlling and driving the single cell packaging instrument to operate.
It should be explained that the chip is a microfluidic chip for producing single-cell multiparticulate droplets.
Further, the chip placing module comprises a chip placing plate, a drawing door module and a pressing module; the chip placing plate is used for fixedly placing chips, and the chip placing plate is fixed on the pull door module and can realize pull reciprocating motion along with the pull door module.
In some modes, the chip placing plate is provided with a spring buckle, and after the chip is placed in the chip placing plate, the spring buckle can automatically clamp and position.
Furthermore, the pressing module is used for pressing down the sealing chip, and communicating the fluid input slot on the chip with the electric proportional valve of the air circuit module, and the electric proportional valve provides air pressure for the fluid input slot on the chip.
Because the chip relates to multiple fluids, different fluids need different air pressures, so the air path module needs to be provided with a plurality of electric proportional valves at the periphery to respectively and independently provide different and stable air pressures for different fluids.
Furthermore, an air pressure plate in direct contact with the chip is arranged in the pressing module, and three air pressure passages including a first fluid air pressure passage, a second fluid air pressure passage and a third fluid air pressure passage are arranged on the air pressure plate.
Furthermore, three fluid input grooves are arranged on the chip, namely a first fluid input groove, a second fluid input groove and a third fluid input groove, and each input groove can be filled with corresponding fluid in advance; the three air pressure channels on the air pressure plate can be respectively connected with the three fluid input grooves on the chip in a sealing way.
In some embodiments, there may be a plurality of fluid input slots on the chip, for example, four first fluid input slots, four second fluid input slots and four third fluid input slots, so that the four first fluid input slots may be simultaneously connected to the first fluid pneumatic passages on the pneumatic plate, the four second fluid input slots may be simultaneously connected to the second fluid pneumatic passages on the pneumatic plate, and the four third fluid input slots may be simultaneously connected to the third fluid pneumatic passages on the pneumatic plate.
In some embodiments, the three fluids are an oil phase, a cell suspension, and a suspension of encoded microspheres, respectively.
Furthermore, the gas circuit module is provided with three electric proportional valves which respectively convey gas pressure to the three fluid input grooves on the chip through three gas pressure passages on the gas pressure plate, so that the fluid in the input grooves is pressed into the liquid drop generator of the chip.
In order to match with the three fluid input grooves on the chip, the air circuit module is required to provide three different and stable air pressures, so that the air circuit module is provided with three electric proportional valves around the periphery of the air pump, and the three electric proportional valves are respectively connected with the passages of the three fluid input grooves of the chip through three air pressure passages on the air pressure plate and are mutually independent.
Furthermore, one or more sets of fluid input grooves are arranged on the chip, and each set of fluid input groove comprises three fluid input grooves which are a first fluid input groove, a second fluid input groove and a third fluid input groove respectively; one or more sets of droplet generators are arranged in the chip, and each set of droplet generator corresponds to one set of fluid input groove.
By arranging multiple sets of drop generators on the chip simultaneously, each set of drop generator is in corresponding communication with a set of input slots on the chip containing three fluids. Meanwhile, a first fluid input groove in each set of liquid drop generators is connected with a first fluid air pressure passage on the air pressure plate in series, a second fluid input groove in each set of liquid drop generators is connected with a second fluid air pressure passage on the air pressure plate in series, a third fluid input groove in each set of liquid drop generators is connected with a third fluid air pressure passage on the air pressure plate in series, and a fourth fluid input groove in each set of liquid drop generators is connected with a second fluid air pressure passage on the air pressure plate in series, so that the efficient utilization of the air path module is realized, the resources are saved, the high-flux generation of liquid drops is realized, and the liquid drop generation efficiency is practically improved.
Furthermore, four sets of liquid drop generators and four sets of fluid input grooves corresponding to the four sets of liquid drop generators are arranged on the chip; the pressing module is driven by a screw rod stepping motor to move up and down and is positioned through a guide rail.
According to the size and the position of the chip, four sets of liquid drop generators can be arranged on the same chip, the high-efficiency liquid drop generation efficiency which is 4 times that of a common liquid drop generator is realized, each fluid can share one electric proportional valve, and the high efficiency and the energy saving are really realized.
Furthermore, the drawing door module is driven by a motor to realize drawing movement by a gear and a rack, and is positioned by the aid of a guide rail; the initial position and the end position of the drawing door module are sensed and positioned by the photoelectric sensor.
In some modes, the guide rail is provided with two, sets up in both sides separately, and the help must be better fixed and fix a position, makes the pull door module more stable at the pull in-process, can not appear the problem of rolling.
Whether the drawing door module is at the initial position or the end position is confirmed through the photoelectric sensor, so that whether the drawing motion is started or stopped is determined.
In some modes, the circuit module is mainly a driving system for controlling a motor, and comprises a driver, a regenerative discharging clamp, a medical power supply module for providing power, a singlechip mainboard for controlling the whole machine and the like.
In some modes, the air path module mainly comprises an air pump, an air release valve, an electric proportional valve, a connector and the like.
Furthermore, the single cell packaging instrument further comprises a shell, and a display screen is arranged on the shell. The shell is arranged on the main frame, can be made of plastic materials, and can also be provided with an LED lamp.
The utility model provides a unicellular parcel appearance has following beneficial effect:
1. the chip comprises a plurality of sets of liquid drop generators, and single-cell multi-particle liquid drops can be generated in a high-throughput manner;
2. when the liquid drops are produced in high throughput, each fluid shares one electric proportional valve, so that high efficiency and energy conservation are really realized;
3. the automation degree is higher;
4. simple structure, convenience, high efficiency, low cost and easy popularization.
Drawings
FIG. 1 is a schematic diagram of the external structure of a single-cell packaging instrument in example 1;
FIG. 2 is a schematic diagram showing the internal structure of the single-cell packaging instrument in example 1;
FIG. 3 is a schematic structural diagram of an external appearance of the chip in embodiment 1;
fig. 4 is a schematic view of a flow channel structure of a droplet generator inside a chip in embodiment 1;
fig. 5 is a schematic structural view of the gas panel in embodiment 1;
fig. 6 is a schematic structural view of a push-down module in embodiment 1;
fig. 7 is a schematic structural view of a sliding door module in embodiment 1.
Detailed Description
In the following, preferred embodiments of the present invention will be described in further detail with reference to the accompanying drawings, it being noted that the embodiments described below are intended to facilitate understanding of the present invention without any limiting effect. The raw materials and the equipment used in the embodiment of the utility model are known products and are obtained by purchasing products sold in the market.
The schematic diagrams of the single cell encapsulation instrument provided in this embodiment are shown in fig. 1 to 5, wherein fig. 1 is a schematic diagram of an appearance structure of the single cell encapsulation instrument; FIG. 2 is a schematic diagram of the internal structure of the single-cell encapsulation instrument; FIG. 3 is a schematic diagram of an external structure of a chip; FIG. 4 is a schematic view of a flow channel structure of a droplet generator inside a chip; fig. 5 is a schematic structural view of the gas panel.
As shown in fig. 1 and 2, the single-cell packaging instrument provided in this embodiment includes a housing 1, and an internal chip 2, a chip placement module 3, a circuit module 4, and an air circuit module 5, wherein a droplet generator 6 (fig. 4) is disposed in the chip 2, and a fluid input slot 7 (fig. 3) is disposed on the chip 2; the chip placing module 3 is used for placing the chip 2 in a sealing mode, the gas circuit module 5 is used for providing air pressure for the fluid input groove 7 on the chip 2, and the circuit module 4 is used for controlling and driving the single cell packaging instrument to operate. Referring to fig. 4, the chip 2 is a microfluidic chip for producing single-cell multi-particle droplets, and the droplet generator 6 inside the chip 2 is a fluid mixer for producing single-cell multi-particle droplets, and mainly by first joining the encoded microsphere suspension in the encoded microsphere flow channel 11 and the cell suspension in the cell flow channel 12 at the first cross-shaped flow channel 14, and then cutting and wrapping the encoded microsphere suspension and the cell suspension into single-cell encoded microsphere droplets at the second cross-shaped flow channel 15 by the oil phase in the oil phase flow channel 13.
As shown in fig. 2, the chip placement module 3 includes a chip placement plate 8, a sliding door module 9 and a pressing module 10; the chip placing plate 8 is used for fixedly placing the chips 2, and the chip placing plate 8 is fixed on the sliding door module 9 and can move in a reciprocating manner along with the sliding door module 9. The chip is placed module 3 accessible various fixed mode and is made chip 2 detachably fix on chip places board 8, if paste, magnetic force adsorb, the buckle is fixed etc. in this embodiment, the chip is placed and is equipped with spring buckle 9 on the board 8, puts into the chip when chip 2 and places the board 8 after, spring buckle 9 can die the location by the automatic card. The pressing module 10 is used for pressing the sealing chip 2, and makes the fluid input slot 7 on the chip 2 communicate with the electric proportional valve 16 of the air path module 5, and the electric proportional valve 16 provides air pressure to the fluid input slot 7 on the chip 2. Since the chip 2 contains a plurality of fluids, and different fluids require different air pressures, the air path module 5 needs to be provided with a plurality of electric proportional valves 16 at the periphery to provide different and stable air pressures for different fluids.
As shown in fig. 2 and 5, the push-down module 10 is provided with an air pressure plate 17 directly contacting with the chip 2, and the air pressure plate 17 is provided with three air pressure passages, including a first fluid air pressure passage 18, a second fluid air pressure passage 19 and a third fluid air pressure passage 20. The chip 2 is provided with three fluid input grooves 7, namely a first fluid input groove 21, a second fluid input groove 22 and a third fluid input groove 23, wherein each input groove can be filled with corresponding fluid in advance, for example, the first fluid input groove 21 is filled with oil phase, the second fluid input groove 22 is filled with cell suspension, the third fluid input groove 23 is filled with coding microsphere suspension, and meanwhile, a liquid drop output groove 34 is also arranged; when the pressure plate 17 presses on the chip 2, the three pressure channels on the pressure plate 17 can be respectively connected with the three fluid input grooves on the chip 2 in a sealing manner.
As shown in FIG. 3, there may be a plurality of each fluid input slot 7 on the chip 2, and in this embodiment, there are four first fluid input slots 21, four second fluid input slots 22 and four third fluid input slots 23. The four first fluid input tanks 21 can be simultaneously connected with the first fluid air pressure passages 18 on the air pressure plate 17, and air pressure is input through the first fluid air pressure passages 18 to press the oil phase in the first fluid input tanks 21 into the oil phase flow channel 11 in the droplet generator 6; the four second fluid input grooves 22 can be simultaneously connected with the second fluid pressure passage 19 on the pressure plate 17, and the air pressure is input through the second fluid pressure passage 19 to press the cell suspension in the second fluid input grooves 22 into the cell flow channel 12 in the droplet generator 6; the four third fluid input channels 23 may be simultaneously connected to the third fluid pneumatic passage 20 in the pneumatic plate 17 to force the encoded microsphere suspension in the third fluid input channels 23 into the encoded microsphere fluid channels 13 in the droplet generator 6.
As shown in fig. 2, the air circuit module 5 is provided with three electric proportional valves 16, which respectively supply air pressure to the three fluid input grooves on the chip 2 through three air pressure passages on an air pressure plate 17, so as to press the fluid in the fluid input grooves 7 into the droplet generators 6 of the chip 2. The three electric proportional valves 16 are provided to cooperate with the three fluid input slots of the chip 2, because the three fluids require the air path module 5 to provide three different and stable air pressures, so the air path module 5 is provided with the three electric proportional valves 16 around the periphery of the air pump, so that the three electric proportional valves are respectively connected with the three fluid input slots of the chip 2 through the three air pressure passages on the air pressure plate 17 and are independent of each other.
Referring to FIG. 3, one or more sets of fluid input slots are provided on the chip 2, each set of fluid input slots includes three fluid input slots, namely a first fluid input slot 21, a second fluid input slot 22 and a third fluid input slot 23; one or more sets of droplet generators 6 are provided in the chip 2, each set of droplet generators 6 corresponding to a respective set of fluid input channels. By providing multiple sets of drop generators 6 simultaneously on chip 2, each set of drop generators 6 is in communication with a set of input channels on chip 2 containing three fluids. Drop generators 6 are located inside chip 2, as fluid microchannels, with no drop generators 6 inside visible from the outer surface of chip 2. Preferably, four sets of droplet generators 6 (fig. 4) and four sets of fluid input slots (fig. 3) corresponding to the four sets of droplet generators 6 are disposed on the chip 2, so as to obtain fig. 5, 4 first fluid input slots 21 in each set of droplet generators 6 are connected in series with the first fluid pneumatic passage 18 on the pneumatic plate 17, 4 second fluid input slots 22 in each set of droplet generators 6 are connected in series with the second fluid pneumatic passage 19 on the pneumatic plate 17, and 4 third fluid input slots 23 in each set of droplet generators 6 are connected in series with the third fluid pneumatic passage 20 on the pneumatic plate 17, thereby achieving efficient utilization of the gas circuit module 5, saving resources, achieving high-throughput generation of droplets, and practically improving droplet generation efficiency.
As shown in fig. 6, the push-down module 10 is moved up and down by a lead screw stepping motor 24 and is positioned by a guide rail 25. According to the size and the position of the chip 2, four sets of the liquid drop generators 6 can be arranged on the same chip 2, the high-efficiency liquid drop generation efficiency which is 4 times that of a common liquid drop generator is realized, each fluid can share one electric proportional valve 16, and the high efficiency and the energy saving are really realized.
As shown in fig. 7, the door-pulling module 9 is moved by a motor 26 driving a gear 27 and a rack 28, and is positioned by a guide rail 29; the initial position and the end position of the sliding door module 9 are sensed and positioned by a photoelectric sensor. The two guide rails 29 are respectively arranged on two sides, so that better fixation and positioning are facilitated, the drawing door module 9 is more stable in the drawing process, and the problem of left-right shaking cannot occur. Whether the drawing motion is started or stopped is determined by confirming whether the door module 9 is at the initial position or the end position by a photoelectric sensor.
Preferably, the circuit module 4 is mainly a driving system for controlling the motor, and includes a driver, a regenerative discharging clamp, a medical power supply module for providing power, and a single chip microcomputer motherboard for controlling the whole machine.
Preferably, the air path module 5 mainly includes an air pump, an air release valve, an electric proportional valve, a connector, and the like.
As shown in FIG. 1, the instrument for packaging single cell further comprises a housing 1, and a display screen 30 is disposed on the housing 1. The housing 1 is arranged on the main frame 31, can be made of plastic, and can be further provided with an LED lamp for illumination, and the drawing module can extend out of a movable window 33 of the housing 1 under the control of a switch 32 for replacing the chip 2.
In this embodiment, the high-throughput single-cell encapsulation instrument provided in example 1 is compared with the existing single-cell encapsulation instrument which only includes one droplet generator and is prepared by manually connecting the chip with an air pressure device, so that the channel air pressures of the two sets of single-cell encapsulation instruments are consistent (the pressure at the inlet of the coding microsphere channel is controlled to be 3.0psi, the pressure at the inlet of the cell channel is controlled to be 4.5psi, and the pressure at the inlet of the oil phase channel is controlled to be 8.5psi), and the instrument is continuously operated at the same time, and the number of the prepared effective droplets, the ratio of the effective droplets, and the power consumption are examined, and the results are shown in table 1.
TABLE 1 comparison of the applications of Single cell encapsulation instruments
| Serial number | Total number of droplets | Effective number of drops | Effective droplet fraction (%) | Consumption of electricity (kWh) |
| 1 (example 1) | 67522 | 48886 | 72.40 | 1.3 |
| 2 (simple type) | 15081 | 10760 | 71.35 | 1.1 |
It can be seen from table 1 that adopt the unicellular parcel appearance of the high flux that embodiment 1 provided, can obtain more effective liquid drops in the same time, but power consumption does not have the difference hardly, it is visible the utility model provides a unicellular parcel appearance can not only realize that high flux prepares unicellular singly to encode the microballon liquid drop, and can reduce energy resource consumption by a wide margin, more energy-efficient.
Although the present invention is disclosed above, the present invention is not limited thereto. For example, the application range of the micro-fluidic field can be expanded. Various changes and modifications may be effected by one skilled in the art without departing from the spirit and scope of the invention, as defined in the appended claims.
Claims (10)
1. A single cell packaging instrument is characterized by comprising a chip, a chip placing module, a circuit module and a gas circuit module, wherein a liquid drop generator is arranged in the chip, and a fluid input groove is arranged on the chip; the chip placing module is used for placing chips in a sealed mode, the gas circuit module is used for providing air pressure for the fluid input grooves in the chips, and the circuit module is used for controlling and driving the single cell packaging instrument to operate.
2. The single cell packaging instrument of claim 1, wherein the chip placement module comprises a chip placement plate, a pull door module and a push-down module; the chip placing plate is used for fixedly placing chips, and the chip placing plate is fixed on the pull door module and can realize pull reciprocating motion along with the pull door module.
3. The single cell packaging instrument of claim 2, wherein the pressing module is used for pressing down the sealed chip and communicating the fluid input slot on the chip with the electric proportional valve of the air channel module, and the electric proportional valve provides air pressure to the fluid input slot on the chip.
4. The single cell encapsulation instrument of claim 3, wherein the pressing module has an air pressure plate directly contacting the chip, and the air pressure plate has three air pressure channels including a first fluid air pressure channel, a second fluid air pressure channel and a third fluid air pressure channel.
5. The single cell packaging instrument of claim 4, wherein the chip is provided with three fluid input slots, namely a first fluid input slot, a second fluid input slot and a third fluid input slot, each of which is filled with a corresponding fluid in advance; the three air pressure passages on the air pressure plate can be respectively connected with the three fluid input grooves in a sealing way.
6. The single cell packaging instrument of claim 5, wherein the air channel module is provided with three electric proportional valves, and air pressure is respectively transmitted to the three fluid input slots on the chip through three air pressure channels on the air pressure plate, so that the fluid in the input slots is pressed into the droplet generator of the chip.
7. The single-cell encapsuiator of claim 6, wherein the chip has one or more sets of fluid input slots, each set of fluid input slots comprising three fluid input slots, a first fluid input slot, a second fluid input slot and a third fluid input slot; one or more sets of droplet generators are provided in the chip, each set of droplet generators corresponding to a respective set of fluid input channels.
8. The single cell packaging instrument of claim 7, wherein four sets of droplet generators and four sets of fluid input slots corresponding to the four sets of droplet generators are disposed on the chip; the pressing module is driven by a screw rod stepping motor to move up and down and is positioned through a guide rail.
9. The instrument as claimed in claim 8, wherein the door module is driven by a motor to move the gear and rack for a pulling motion, and is positioned by a guide rail; the initial position and the end position of the drawing door module are sensed and positioned by the photoelectric sensor.
10. The single cell encapsulation instrument of claim 9, further comprising a housing, the housing having a display screen.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123283480.1U CN217297855U (en) | 2021-12-24 | 2021-12-24 | Single cell parcel appearance |
| US17/715,426 US20230201834A1 (en) | 2021-12-24 | 2022-04-07 | Droplet generator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123283480.1U CN217297855U (en) | 2021-12-24 | 2021-12-24 | Single cell parcel appearance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217297855U true CN217297855U (en) | 2022-08-26 |
Family
ID=82921795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123283480.1U Active CN217297855U (en) | 2021-12-24 | 2021-12-24 | Single cell parcel appearance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217297855U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117327774A (en) * | 2023-11-03 | 2024-01-02 | 广州君瑞康生物科技有限公司 | Single cell rapid measurement analysis method |
-
2021
- 2021-12-24 CN CN202123283480.1U patent/CN217297855U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117327774A (en) * | 2023-11-03 | 2024-01-02 | 广州君瑞康生物科技有限公司 | Single cell rapid measurement analysis method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103097883B (en) | The monosomic chip providing sample to input to result output processing and manufacture method | |
| CN111057638B (en) | Sample processing device and method, and digital PCR system including the same | |
| Lee et al. | Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control | |
| US10272436B2 (en) | Assay cartridge valve system | |
| CN109289949B (en) | Full-automatic micro-droplet emulsion generation device and generation method thereof | |
| CN217297855U (en) | Single cell parcel appearance | |
| CN1441703A (en) | Microreactor | |
| CN101696916B (en) | Device for analyzing and sieving liquid drops based on integrative sampling probe of chip | |
| CN101236175B (en) | Programmable power supply system dedicated for chip electrophoresis | |
| CN102047124A (en) | Fluid processing and transfer using inter-connected multi-chamber device | |
| EP4170008A1 (en) | Sample processing and detection apparatus and application thereof | |
| CN208224063U (en) | Chip reaction unit and water quality many reference amounts detection device based on chip reaction unit | |
| CN103386338A (en) | Micro-fluidic combined chemical reaction chip | |
| CN107282144A (en) | The detection method of micro-fluidic chip and copper ion | |
| CN205152235U (en) | A micro -fluidic chip , detecting system and device for gene somatotype detects | |
| CN110470601A (en) | Water quality many reference amounts detection device and detection method based on chip reaction unit | |
| CN115651807B (en) | Nucleic acid detection chip and nucleic acid detection method | |
| CN103595864A (en) | Microfluidic chip liquid control system based on wireless mobile intelligent terminal | |
| CN218339833U (en) | Liquid transfer, multi-channel liquid transfer, automatic liquid extraction and transfer and multi-channel automatic liquid extraction and transfer device | |
| CN103586094B (en) | Micro-fluidic chip with circulation mixed structure and circulation mixed method | |
| CN111760602B (en) | Small-sized microfluidic single-cell packaging instrument | |
| CN115044454A (en) | Nucleic acid extraction apparatus | |
| CN211035866U (en) | Plunger type nucleic acid detection integrated card box | |
| CN106378214A (en) | Open-type U-shaped bipolar-electrode electrochemiluminescence cloth chip and manufacture method and application thereof | |
| CN100385227C (en) | Piezo-electric driving type micro mixed chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |