CN217407342U - Liquid culture device - Google Patents
Liquid culture device Download PDFInfo
- Publication number
- CN217407342U CN217407342U CN202221185811.XU CN202221185811U CN217407342U CN 217407342 U CN217407342 U CN 217407342U CN 202221185811 U CN202221185811 U CN 202221185811U CN 217407342 U CN217407342 U CN 217407342U
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- Prior art keywords
- test tube
- incubator
- floating plate
- culture
- water outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000009630 liquid culture Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000011521 glass Substances 0.000 claims abstract description 9
- 239000000178 monomer Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 7
- 244000205754 Colocasia esculenta Species 0.000 description 4
- 235000006481 Colocasia esculenta Nutrition 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000006286 nutrient intake Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012883 rooting culture medium Substances 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a liquid culture apparatus, it includes monomer culture apparatus and shipment device, monomer culture apparatus includes: the upper part of the glass test tube is provided with a test tube port thread, and the test tube bottle cap is provided with a thread port matched with the test tube port thread; the center of the test tube bottle cap is provided with an air hole, and the air hole is covered with a breathable film; the shipping device includes: the culture box comprises an incubator and an upper cover, wherein a floating plate is transversely clamped in the incubator and provided with a plurality of floating plate holes, the lower part of the incubator is provided with a water outlet, and a water outlet cover is arranged on the water outlet; the upper cover is provided with a vent hole.
Description
Technical Field
The utility model belongs to the technical field of culture dish and specifically relates to a liquid culture apparatus.
Background
The taro tissue culture process is divided into three stages of bud induction and differentiation, tissue culture seedling subculture proliferation and rooting culture, and a solid culture medium is usually used in the tissue culture process. In order to prevent nutrient consumption in the culture medium in the tissue culture process, the tissue culture seedlings need to be transferred to a new culture set regularly, the operation is troublesome, and pollution is easily caused. When the obtained tissue culture seedlings can not be transplanted in time due to crop rotation or season, the growth of the leaf stalks of the tissue culture seedlings is deformed and the space of a tissue culture room is occupied because the space of the tissue culture container is limited. Meanwhile, when the obtained tissue culture seedlings need to be transported in a long distance, the transportation with the tissue culture container occupies a large space, and the transportation cost is high.
The prior art has the following defects: (1) the solid culture medium is used in the subculture propagation and rooting culture stages of the tissue culture seedling, so that the tissue culture seedling needs to be transferred to a new culture set periodically to prevent nutrient consumption in the culture medium, the operation is troublesome, and the pollution is easy to cause. (2) When the obtained tissue culture seedlings can not be transplanted in time due to stubble or season, the growth of the petioles of the tissue culture seedlings is deformed due to the limited space of the tissue culture container, and the tissue culture container occupies the space of the tissue culture room. Meanwhile, when the obtained tissue culture seedlings need to be transported in a long distance, the transportation with the tissue culture container occupies a large space, and the transportation cost is high.
SUMMERY OF THE UTILITY MODEL
The utility model provides a liquid culture apparatus aiming at the problems existing in the background technology.
The technical scheme is as follows:
a liquid culture device comprises a monomer culture device and a loading device,
the monomer culture apparatus includes:
the upper part of the glass test tube is provided with a test tube port thread, and the test tube bottle cap is provided with a thread port matched with the test tube port thread; the center of the test tube bottle cap is provided with an air hole, and the air hole is covered with a breathable film;
the shipping device includes:
the floating plate is transversely clamped in the incubator and is provided with a plurality of floating plate holes, the lower part of the incubator is provided with a water outlet, and the water outlet is provided with a water outlet cover; the upper cover is provided with a vent hole.
Preferably, one side of the upper cover is hinged with one side of the incubator.
Preferably, the upper cover is formed by splicing two plates, and the two plates are connected through a hinge.
Preferably, the incubator is made of transparent PP.
Preferably, the floating plate is made of PEP.
Preferably, the material of the drain cover is rubber.
Preferably, the aperture of the floating plate hole is 10 mm.
The beneficial effects of the utility model
The utility model discloses need not use the solid medium in taro tissue culture seedling subculture proliferation and rooting culture stage for taro subculture proliferation culture stage culture medium nutrient supplement is more convenient, simplifies the tissue culture seedling and trains the seedling and transplant the step. The utility model provides a can preserve the device more than several months with the preceding water planting of the tissue culture seedling transplantation of obtaining, make things convenient for tissue culture seedling to transplant crop arrangement and long distance transport.
Drawings
FIG. 1 is a schematic view of the structure of the monomer culturing device of the present invention
FIG. 2 is a schematic view of the structure of the loading and transporting device of the present invention
Detailed Description
The present invention will be further explained with reference to the following examples, but the scope of the present invention is not limited thereto:
a liquid culture device comprises a monomer culture device and a loading device,
referring to fig. 1, the monomer culture apparatus includes:
the upper part of the glass test tube 11 is provided with a test tube port thread 100, and the test tube bottle cap 9 is provided with a thread port matched with the test tube port thread 100; the center of the test tube bottle cap 9 is provided with an air vent 13, and the air vent 13 is covered with an air permeable film 14;
referring to fig. 2, the shipping apparatus includes:
the culture box comprises an incubator 8 and an upper cover 3, wherein a floating plate 5 is transversely clamped in the incubator 8, a plurality of floating plate holes 4 are formed in the floating plate 5, a water outlet 7 is formed in the lower part of the incubator 8, and a water outlet cover 6 is arranged on the water outlet 7; the upper cover 3 is provided with a vent hole 2.
Preferably, one side of the upper cover 3 is connected to one side of the incubator 8 by a hinge 1.
Preferably, the upper cover 3 is formed by splicing two plates, and the two plates are connected through a hinge 1.
Preferably, the incubator 8 is made of a transparent PP material.
Preferably, the material of the floating plate 5 is PEP.
Preferably, the drain cover 6 is made of rubber.
Preferably, the aperture of the floating plate holes 4 is 10 mm.
The using method comprises the following steps:
in the example, the hormone formula used in the tissue culture process of the taro is MS +6-BA 2.0mg/ml, NAA0.02mg/ml +30g/L sucrose and the pH value is 5.8, wherein the induction culture medium is a solid culture medium, the subculture multiplication and rooting culture medium is a liquid culture medium, the multiplication coefficient of the hormone formula is 5.5, and the plant grows robustly.
Example MS liquid medium was used as a secondary proliferation and rooting medium, a glass test tube 11 with a vent hole of 30mm in inner diameter and 80mm in height was used as a tissue culture container, and a gas permeable membrane 14 was covered on the vent hole 13. In the process of subculture, when the tissue culture seedling needs to be supplemented with nutrients, the air-permeable membrane 14 is wiped by an alcohol cotton ball, the glass test tube 11 is tilted, the culture medium 12 in the glass test tube 11 is sucked out by a sterile syringe, and then the sterilized fresh liquid culture medium is added into the glass bottle by the sterile syringe. A new breathable film is attached to the pinhole to prevent pollution. The nutrient supplement of the culture medium in the tissue culture process is convenient.
In the embodiment, a transparent PP culture box 8 (with a cover and a size of 450mm in length, 35mm in width and 400mm in height) is matched with an EPE foam plate as a floating support, namely a floating plate 5 (with a size of 400mm in length and 30mm in width, a pore diameter of a hole 4 of the floating plate is 10mm, and a pore space is 20mm), to perform water culture on culture seedlings, and the water culture solution can be selected from clear water or water culture nutrient solution according to needs. The utility model discloses in reduce the tissue culture seedling through water culture case water culture method and preserve occupation space big, and can effectively coordinate tissue culture seedling and plant the stubble, aspect tissue culture seedling long distance transport simultaneously.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.
Claims (7)
1. A liquid culture device is characterized in that the device comprises a monomer culture device and a loading device,
the monomer culture apparatus comprises:
the test tube comprises a glass test tube (11), wherein the upper part of the glass test tube (11) is provided with a test tube port thread (100), and a test tube bottle cap (9) is provided with a thread port matched with the test tube port thread (100); the center of the test tube bottle cap (9) is provided with an air vent (13), and the air vent (13) is covered with an air permeable film (14);
the shipping device includes:
the cultivation box comprises an incubator (8) and an upper cover (3), wherein a floating plate (5) is transversely clamped in the incubator (8), a plurality of floating plate holes (4) are formed in the floating plate (5), a water outlet (7) is formed in the lower part of the incubator (8), and a water outlet cover (6) is arranged on the water outlet (7); the upper cover (3) is provided with a vent hole (2).
2. Device according to claim 1, characterized in that one side of the upper lid (3) is linked to one side of the incubator (8) by means of a hinge (1).
3. Device according to claim 1, characterized in that the upper lid (3) is made by splicing two plates, which are connected by a hinge (1).
4. Device according to claim 1, characterized in that the incubator (8) is made of transparent PP.
5. Device according to claim 1, characterized in that the floating plate (5) is made of PEP.
6. Device according to claim 1, characterized in that the material of the drain cover (6) is rubber.
7. The device according to claim 1, characterized in that the aperture of the floating plate holes (4) is 10 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221185811.XU CN217407342U (en) | 2022-05-17 | 2022-05-17 | Liquid culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221185811.XU CN217407342U (en) | 2022-05-17 | 2022-05-17 | Liquid culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217407342U true CN217407342U (en) | 2022-09-13 |
Family
ID=83187263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221185811.XU Active CN217407342U (en) | 2022-05-17 | 2022-05-17 | Liquid culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217407342U (en) |
-
2022
- 2022-05-17 CN CN202221185811.XU patent/CN217407342U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Pinghu Yinfeng Fruit and Vegetable Professional Cooperative Assignor: Jiaxing Academy of Agricultural Sciences Contract record no.: X2023330000812 Denomination of utility model: Liquid culture device Granted publication date: 20220913 License type: Common License Record date: 20231107 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Pinghu Sweet Family Farm Co.,Ltd. Assignor: Jiaxing Academy of Agricultural Sciences Contract record no.: X2023330000859 Denomination of utility model: Liquid culture equipment Granted publication date: 20220913 License type: Common License Record date: 20231116 |