CN217398846U - Fermentation cylinder adds fungus device - Google Patents
Fermentation cylinder adds fungus device Download PDFInfo
- Publication number
- CN217398846U CN217398846U CN202220301183.0U CN202220301183U CN217398846U CN 217398846 U CN217398846 U CN 217398846U CN 202220301183 U CN202220301183 U CN 202220301183U CN 217398846 U CN217398846 U CN 217398846U
- Authority
- CN
- China
- Prior art keywords
- box body
- fermentation tank
- bacteria
- adding device
- box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a fermentation cylinder adds fungus device belongs to synthetic biology fermentation technical field. The utility model comprises a box body (1), aseptic gloves (2), a tubular storage channel (3) and a sealing cover (4); the top wall of the box body (1) is made of elastic material; at least one of the side walls of the box body (1) is made of transparent material; cuffs of the two sterile gloves (2) are respectively connected with two circular holes on the side wall of the box body (1) in a sealing way; the sterile gloves (2) extend into the box body (1); the side wall of the box body (1) is provided with a cylindrical object placing channel (3) which faces the interior of the box body; the sealing cover (4) covers the cylindrical object placing channel (3). The utility model discloses a shake the bottle and put into fermentation cylinder and add the fungus device back to be equipped with the bacterial sealed, shift and add the fungus in-process, reduce miscellaneous fungus pollution. Simple structure, convenient operation greatly reduces the economic loss that miscellaneous fungus pollutes and brings.
Description
Technical Field
The invention relates to the technical field of synthetic biology fermentation, in particular to a fermentation tank bacterium adding device.
Background
Synthetic biology provides a brand new research strategy for life science research, biotechnology development and bioengineering application on the basis of biology and engineering. People use escherichia coli, yeast, blue algae and the like as chassis cells, and produce clean, efficient and sustainable chemical and biological energy products by fermenting and taking biomass, carbon dioxide and the like as raw materials through system design and modification.
Fermentors are synthetic biologically used devices for fermentation of engineered microorganisms. When the bacteria are added into the fermentation tank, the bacteria in the shake flask are added into the fermentation tank through the feed inlet of the fermentation tank after the flame or alcohol sterilization is carried out near the feed inlet of the fermentation tank.
As only the flame or alcohol sterilization is carried out near the feed inlet of the fermentation tank, the mixed bacteria can easily enter the fermentation tank through the feed inlet of the fermentation tank during the bacteria adding. In the process of transferring the shake flask filled with the bacteria, the outside of the shake flask filled with the bacteria is easy to adhere with the mixed bacteria, and when the bacteria are added into the fermentation tank, the mixed bacteria adhered to the outside of the shake flask filled with the bacteria easily enter the fermentation tank to infect the fermentation process, thereby bringing great loss.
Disclosure of Invention
In order to solve the technical problem, the utility model provides a fermentation cylinder adds fungus device, put thing passageway and sealed lid including box, aseptic gloves, tube-shape, can also place the box, shake a bottle fixed rope, article and place box and article fixed rope including shaking the bottle. The shake flask filled with the bacteria can be placed in the sealed fermentation tank bacteria adding device, so that the shake flask filled with the bacteria is prevented from being adhered with the mixed bacteria. Simple structure, convenient operation, and reduced contamination caused by bacteria.
In order to achieve the above object, the utility model provides a following scheme:
a fermentation tank bacteria adding device comprises a tank body, sterile gloves, a cylindrical object placing channel and a sealing cover; the top wall of the box body is made of elastic material; at least one of the side walls of the box body is made of a transparent material; the side wall of the box body is provided with two circular holes; the number of the sterile gloves is two, and cuffs of the two sterile gloves are respectively in sealing connection with two circular holes in the side wall of the box body; the sterile gloves extend into the box body; the side wall of the box body is provided with a cylindrical object placing channel which is arranged towards the inside of the box body; the sealing cover covers the cylindrical object placing channel.
Furthermore, the cylindrical object placing channel is externally provided with a bacterium separating cylinder communicated with the cylindrical object placing channel, the bacterium separating cylinder is of a cylindrical structure, is fixedly arranged on the outer wall of the box body, and is concentrically arranged with the cylindrical object placing channel.
Furthermore, the material of separating the fungus section of thick bamboo is elasticity.
Furthermore, the fermentation tank bacteria adding device is also provided with a shake flask placing box, and the shake flask placing box is arranged on the inner bottom surface of the box body.
Furthermore, the fermentation tank bacterium adding device is also provided with a shake flask fixing rope, the shake flask fixing rope is arranged at the bottom of the shake flask placing box, and the shake flask fixing rope is made of elastic materials.
Furthermore, the fermentation tank bacterium adding device is also provided with an article placing box, and the article placing box is arranged on the inner bottom surface of the box body.
Furthermore, the fermentation tank bacteria adding device is also provided with an article fixing rope, and the article fixing rope is arranged at the bottom of the article placing box.
The utility model has the advantages that:
in an aseptic environment, the shake flask filled with the bacteria is placed in a fermentation tank bacteria adding device, and a cylindrical object placing channel of the fermentation tank bacteria adding device is sealed by a sealing cover, so that the shake flask filled with the bacteria is in the aseptic environment in the transfer process, and the adhesion of mixed bacteria in the transfer process of the shake flask filled with the bacteria is avoided.
Transferring the shake flasks filled with the bacteria in the fermentation tank bacteria adding device and the fermentation tank bacteria adding device to the position near a fermentation tank feed inlet, sterilizing the fermentation tank feed inlet, tearing off sterile paper outside a bacteria isolation cylinder of the fermentation tank bacteria adding device, tightly attaching a sealing cover of a storage channel in the cylindrical shape of the fermentation tank bacteria adding device to the feed inlet, opening the sealing cover of the storage channel in the sealed cylindrical shape, and adding the bacteria in the shake flasks into the fermentation tank through the storage channel in the cylindrical shape and the fermentation tank feed inlet. In the process of adding bacteria, the mixed bacteria in the air are reduced and enter the fermentation tank through the feed inlet of the fermentation tank, so that the pollution of the mixed bacteria is reduced.
The utility model discloses a fermentation cylinder adds fungus device can also place the box including shaking a bottle, shake a bottle fixed rope, article and place box and article fixed rope, can place and/or the fixed shake bottle that has the fungus, avoids shaking the bottle of being equipped with the fungus damage in the transfer process, can place and/or article such as fixed transfering loop, tweezers, avoids article to damage the bottle of shaking that is equipped with the fungus in the transfer process.
The utility model discloses simple structure, convenient operation reduces adding the pollution of fungus in-process, greatly reduces the economic loss that miscellaneous fungus pollutes and brings.
Drawings
Fig. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is a schematic longitudinal section view of the cylindrical object-placing channel and the bacteria-isolating cylinder of the present invention;
FIG. 3 is a schematic view of the shake flask placing box and the shake flask fixing rope of the present invention;
in the figure, 1-box body, 2-sterile gloves, 3-sealing cover, 4-inner layer opening, 5-outer layer opening, 6-shake flask placing box, 7-shake flask fixing rope, 8-article placing box and 9-article fixing rope.
Detailed Description
The technical solutions of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a fermentation cylinder adds fungus device, put thing passageway and sealed lid including box, aseptic gloves, tube-shape, can also place the box, shake the fixed rope of bottle, article and place box and the fixed rope of article including shaking the bottle. The shake flask filled with the bacteria can be placed in the sealed fermentation tank bacteria adding device, so that the shake flask filled with the bacteria is prevented from being adhered with the mixed bacteria. Simple structure, convenient operation, and reduced contamination caused by bacteria.
As shown in the figure, the fermentation tank bacteria adding device of the utility model comprises a tank body 1, sterile gloves 2, a cylindrical object placing channel 3 and a sealing cover 4; the top wall of the box body 1 is made of elastic material; at least one of the side walls of the box body 1 is made of transparent material; the side wall of the box body 1 is provided with two circular holes; the number of the sterile gloves 2 is two, and cuffs of the two sterile gloves 2 are respectively connected with two circular holes on the side wall of the box body 1 in a sealing manner; the sterile gloves 2 extend into the box body 1; the side wall of the box body 1 is provided with a cylindrical object placing channel 3 which is arranged towards the inside of the box body; the sealing cover 4 is covered on the cylindrical object placing passage 3.
On a clean bench, the shake flask with bacteria is placed into a fermentation tank bacteria adding device through a cylindrical object placing channel 3, and the cylindrical object placing channel 3 is covered by a sealing cover 4, so that the shake flask with bacteria is in the sealed fermentation tank bacteria adding device, and the shake flask with bacteria is prevented from being adhered with mixed bacteria.
The cylindrical object placing channel 3 is externally provided with a bacteria separating cylinder 5 communicated with the cylindrical object placing channel, and the bacteria separating cylinder 5 is of a cylindrical structure, is fixedly arranged on the outer wall of the box body and is concentric with the cylindrical object placing channel 3. The material of the bacteria-isolating cylinder 5 is elastic.
The bacteria isolation cylinder 5 is sealed by sterile paper, the cylindrical object placing channel 3 is sealed by the sealing cover 7, and the sealing cover 7 on the inner layer cylindrical object placing channel 3 can be ensured to be in a sterile state even if the sterile paper outside the bacteria isolation cylinder 5 is adhered with sundry bacteria in the transferring process of the fermentation tank bacteria adding device. The material that separates a fungus section of thick bamboo 5 is rubber elasticity material, makes the edge that separates a fungus section of thick bamboo 5 can laminate the fermentation cylinder more, avoids miscellaneous fungus to get into better.
The fermentation tank fungus adding device also comprises a shake flask placing box 6, and the shake flask placing box 6 is fixedly arranged on the bottom surface of the interior of the box body 1. The fermentation tank adds fungus device still includes shake a bottle fixed rope 7, shakes a bottle fixed rope 7 and sets up and place the box 6 bottom in shaking a bottle, shakes the material of bottle fixed rope 7 and is the elasticity. The shake flask filled with the bacteria is placed in a shake flask placing box and is fixed by an elastic shake flask fixing rope 7, so that the shake flask filled with the bacteria is prevented from greatly shaking in a fermentation tank bacteria adding device to damage the shake flask filled with the bacteria.
The fermentation tank adds fungus device still is equipped with article and places box 8, and article are placed box 8 and are set up on the bottom surface of the inside of box 1. The object placing box is arranged to prevent objects such as inoculating rings, tweezers and the like from colliding with shake flasks containing bacteria in the same space to damage the shake flasks containing the bacteria. The fermentation tank bacteria adding device further comprises an article fixing rope 9, and the article fixing rope 9 is arranged at the bottom of the article placing box 8 and used for fixing articles such as inoculating loops and tweezers.
The utility model discloses a working process:
wrapping the bacteria adding device of the fermentation tank with paper, placing the fermentation tank in a high-pressure steam sterilization pot for sterilization, placing the fermentation tank on a clean bench, disinfecting the outside of the shake flask filled with bacteria with alcohol, and placing the shake flask on the clean bench. After the hands of an operator are disinfected, tearing packaging paper of the fermentation tank bacterium adding device, observing through the side wall of the glass, extending the hands into sterile gloves 2 of the fermentation tank bacterium adding device, opening sealing covers covering cylindrical object placing channels 3, taking out the hands from the sterile gloves 2, placing shake flasks with bacteria into a box body 1 through the cylindrical object placing channels 3 of the box body 1, placing the shake flasks with bacteria into a shake flask placing box 6, fixing the shake flasks with bacteria by using rubber fixing ropes 7, and avoiding the damage of the shake flasks with bacteria in the transfer process. Observing through the glass side wall, putting the inoculating loop, the tweezers and other objects into the object placing box 8 through the cylindrical object placing channel 3 of the box body 1, and fixing the inoculating loop, the tweezers and other objects by the object fixing rope 9 to prevent the objects from damaging the shake flask containing the bacteria. The hands are put into the sterile gloves 2 of the fermentation tank bacteria adding device, and the cylindrical object placing channel 3 is sealed by the sealing cover 4. Because the top wall of the box body is made of elastic rubber; therefore, both hands can be taken out of the sterile gloves 2 of the fermentation tank sterilization device, and the sterile paper is used for sealing the bacteria isolation cylinder 5.
The fermentation cylinder adds the fungus device and the fermentation cylinder adds shake the bottle and the transfering loop that are equipped with the fungus in the fungus device from the superclean bench, article such as tweezers, add fungus device and fermentation cylinder and add shake the bottle and the transfering loop that are equipped with the fungus in the fungus device with the fermentation cylinder, article such as tweezers are together transferred near fermentation cylinder feed inlet, disinfect to the fermentation cylinder feed inlet, tear the aseptic paper that separates fungus section of thick bamboo 5, see through the glass lateral wall and observe, put thing passageway 3 with the tube-shape and aim at fermentation cylinder to feed inlet, make sealed lid 4 hug closely the feed inlet, stretch into aseptic gloves 2 with the hand, open sealed lid 4 of thing passageway 3 is put to sealed tube-shape, with the transfering loop with the fungus through opening and fermentation cylinder feed inlet add in the fermentation cylinder.
Claims (7)
1. The utility model provides a fermentation cylinder adds fungus device which characterized in that: comprises a box body (1), sterile gloves (2), a cylindrical object placing channel (3) and a sealing cover (4); the top wall of the box body (1) is made of elastic material; at least one of the side walls of the box body (1) is made of transparent material; the side wall of the box body (1) is provided with two circular holes; the number of the sterile gloves (2) is two, and cuffs of the two sterile gloves (2) are respectively in sealing connection with two circular holes in the side wall of the box body (1); the sterile gloves (2) extend into the box body (1); the side wall of the box body (1) is provided with a cylindrical object placing channel (3) which faces the interior of the box body; the sealing cover (4) covers the cylindrical object placing channel (3).
2. The fermentation tank bacteria adding device of claim 1, which is characterized in that: the cylindrical object placing channel (3) is externally provided with a bacterium separating cylinder (5) communicated with the cylindrical object placing channel, the bacterium separating cylinder (5) is of a cylindrical structure and is fixedly arranged on the outer wall of the box body, and the bacterium separating cylinder and the cylindrical object placing channel (3) are arranged concentrically.
3. The fermentation tank bacteria adding device of claim 2, which is characterized in that: the material of the bacteria isolation cylinder (5) is elastic.
4. The fermentation tank bacteria adding device of claim 1, which is characterized in that: the fermentation tank fungus adding device is further provided with a shake flask placing box (6), and the shake flask placing box (6) is arranged on the inner bottom surface of the tank body (1).
5. The fermentation tank bacteria adding device of claim 4, which is characterized in that: the fermentation tank bacterium adding device is further provided with a shake flask fixing rope (7), the shake flask fixing rope (7) is arranged at the bottom of the shake flask placing box (6), and the shake flask fixing rope (7) is elastic.
6. The bacteria adding device for the fermentation tank as claimed in claim 4 or 5, wherein: the fermentation tank bacteria adding device is further provided with an article placing box (8), and the article placing box (8) is arranged on the inner bottom surface of the tank body (1).
7. The fermentation tank bacteria adding device of claim 6, which is characterized in that: the fermentation tank adds fungus device still is equipped with article fixed rope (9), article fixed rope (9) set up the box (8) bottom is placed to the article.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220301183.0U CN217398846U (en) | 2022-02-15 | 2022-02-15 | Fermentation cylinder adds fungus device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220301183.0U CN217398846U (en) | 2022-02-15 | 2022-02-15 | Fermentation cylinder adds fungus device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217398846U true CN217398846U (en) | 2022-09-09 |
Family
ID=83136139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220301183.0U Active CN217398846U (en) | 2022-02-15 | 2022-02-15 | Fermentation cylinder adds fungus device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217398846U (en) |
-
2022
- 2022-02-15 CN CN202220301183.0U patent/CN217398846U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3117998B2 (en) | Apparatus and method for growing anaerobic microorganisms | |
| CN201459137U (en) | Microbial dialysis incubator | |
| CN102154106B (en) | Simple culture method for effective microorganisms (EM) | |
| CN217398846U (en) | Fermentation cylinder adds fungus device | |
| CN203794879U (en) | Portable microbial culture apparatus for culture before use | |
| CN206641033U (en) | Edible fungi inoculation platform | |
| CN201049944Y (en) | Edible mushroom liquid bacterial fermentation device | |
| CN212388030U (en) | Microbial cultivation device | |
| CN208327984U (en) | A kind of microbial culture garden | |
| CN211771277U (en) | Microorganism isolated culture device | |
| CN204918564U (en) | Automatic culture apparatus of waste water treatment microorganism | |
| CN212669674U (en) | Can avoid lactic acid bacteria breeding device of miscellaneous fungus infection | |
| CN207537435U (en) | A kind of culture based devices with anaerobic device | |
| CN101537892B (en) | Method for packaging living microbial agents by using polymer film | |
| CN104651230B (en) | Spontaneous hot liquid cultivates the apparatus and method of active anaerobion | |
| CN204474684U (en) | Spontaneous heating liquid state cultivates the device of active anaerobic microorganism | |
| CN110200241A (en) | A kind of box Concentrated fermentation method of Noni fruit | |
| CN204930334U (en) | A kind of aquatic feeds Rapid Fermentation maker | |
| CN213994358U (en) | Companion formula sterilization apparatus | |
| CN214571903U (en) | Solid microbial inoculant integration production line | |
| CN218680702U (en) | Edible mushroom cross breeding culture utensil | |
| CN213866183U (en) | A multi-functional culture dish for cell culture | |
| CN210496504U (en) | High-temperature steam sterilization inoculation table | |
| CN221822200U (en) | Directional microecological microbial agent apparatus for producing | |
| CN213142016U (en) | Milk powder product microorganism bacterial colony detects culture dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |