CN217377894U - Sealing device of cell tank defoaming system - Google Patents
Sealing device of cell tank defoaming system Download PDFInfo
- Publication number
- CN217377894U CN217377894U CN202220316104.3U CN202220316104U CN217377894U CN 217377894 U CN217377894 U CN 217377894U CN 202220316104 U CN202220316104 U CN 202220316104U CN 217377894 U CN217377894 U CN 217377894U
- Authority
- CN
- China
- Prior art keywords
- hose
- tank
- sealing device
- fermentation cylinder
- storage tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000007789 sealing Methods 0.000 title claims abstract description 34
- 238000000855 fermentation Methods 0.000 claims abstract description 39
- 230000004151 fermentation Effects 0.000 claims abstract description 39
- 239000006260 foam Substances 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000002572 peristaltic effect Effects 0.000 claims description 13
- 238000005303 weighing Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 8
- 238000011109 contamination Methods 0.000 abstract description 7
- 239000002518 antifoaming agent Substances 0.000 description 18
- 229920001971 elastomer Polymers 0.000 description 7
- 239000000806 elastomer Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005273 aeration Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a sealing device of cell jar defoaming system belongs to bioreactor technical field, including fermentation cylinder and storage tank, the output end switch-on of storage tank has the hose, the one end and the fermentation cylinder switch-on of storage tank are kept away from to the hose, the hose is equipped with solenoid valve and power supply along the direction of fermentation cylinder in proper order, the solenoid valve is equipped with the sealing member with the link of hose, the fermentation cylinder tank deck is equipped with two foam electrode sensor of gathering high-low liquid level signal, foam electrode sensor's output electric connection has control center, control center and solenoid valve electric connection, its advantage of this sealing device lies in, it is sealed effectual, prevent that outside bacterium from invading the fermentation cylinder, cause the contamination.
Description
Technical Field
The utility model belongs to the technical field of bioreactor, especially, sealing device of cell jar defoaming system.
Background
In microbial fermentation, carbon dioxide generated by aeration stirring and thallus metabolism and colloidal substances such as protein in a culture solution generate foam in the culture solution, gas exchange is blocked due to excessive foam, the respiration and metabolism of microorganisms are affected, if foam control is not good, a large amount of liquid escaping can be caused, waste and environment pollution are caused, the foam rises to the top of a tank and can seep out from a shaft seal to cause the risk of bacterial contamination, and meanwhile, the excessive foam brings difficulty for subsequent extraction, so that the foam generated in the fermentation process is defoamed by adopting a chemical defoaming method, namely, a defoaming agent is added in a fermentation tank, a hose and a valve are required to control the addition of the defoaming agent, the conventional hose and valve are generally connected through a flange, when the defoaming agent is added, negative pressure shrinkage is generated in the hose, and gaps exist at the connecting end of the hose and the valve easily for a long time, causing external bacteria to enter the hose interior and then causing contamination with antifoam agent entering the fermentor.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: provides a sealing device of a cell tank defoaming system, which solves the problems in the prior art.
The technical scheme is as follows: the utility model provides a sealing device of cell jar defoaming system, includes fermentation cylinder and storage tank, the output switch-on of storage tank has the hose, the one end and the fermentation cylinder switch-on of storage tank are kept away from to the hose, the hose is equipped with solenoid valve and power supply along the direction of fermentation cylinder in proper order, the solenoid valve is equipped with the sealing member with the link of hose, the fermentation cylinder tank deck is equipped with two foam electrode sensor of gathering high low liquid level signal, foam electrode sensor's output electric connection has control center, control center and solenoid valve electric connection.
Through adopting above-mentioned technical scheme, gather height liquid level signal through foam electrode sensor, upload control center's PCL procedure, according to program control solenoid valve on-off time, and then the joining of control defoaming agent, the inside negative pressure that produces of hose is shriveled flat this moment, and the sealing member makes the connection end of hose and solenoid valve be in full bloated state all the time this moment, prevents that there is the gap at the connection end of hose and solenoid valve, prevents that outside bacterium from invading the fermentation cylinder, causes the contamination.
In a further embodiment, the sealing member includes the joint of threaded connection on the solenoid valve, be equipped with the inside lining of annular form on the joint inner wall, be equipped with the annular open slot that supplies the hose to peg graft between inside lining and the joint inner wall, the cover is equipped with wedge elastomer and ejector pad on the hose, the one end that the wedge elastomer is close to the inside lining extends to inside the annular open slot, the one end that annular open slot was kept away from to the wedge elastomer offsets with the ejector pad, connect outside threaded connection has the thread bush, the one end that the wedge elastomer was kept away from to the ejector pad contradicts with the thread bush inner wall.
Through adopting above-mentioned technical scheme, cup joint the hose in the inside lining outside, the inside lining is contradicted the hose inner wall this moment, then rotates the thread bush for the ejector pad promotes the wedge elastomer and fills in annular open slot, and the wedge elastomer holds the hose tightly on the inside lining this moment, when the inside negative pressure that produces of hose, makes the hose be in the state of saturating all the time with the link of solenoid valve, and is sealed effectual.
In a further embodiment, an annular groove is formed in one side, close to the inner wall of the joint, of the lining, a sealing ring is embedded in the annular groove, and the outer ring of the sealing ring is abutted to the inner wall of the hose.
Through adopting above-mentioned technical scheme, improve the leakproofness between hose inner wall and the inside lining through the sealing washer, prevent that the defoaming agent from leaking.
In a further embodiment, the power source is a peristaltic pump and the hose is clamped to the output of the peristaltic pump.
Through adopting above-mentioned technical scheme, carry out extrusion and release in turn through the peristaltic pump to the hose for the inside negative pressure of hose is constantly circulated, and then with the defoaming agent pump sending to the fermentation cylinder in.
In a further embodiment, the top end of the fermentation tank is provided with a sterile push valve, the sterile push valve is communicated with a hose through a sealing piece, and the sterile push valve is positioned at the rear end of the peristaltic pump.
Through adopting above-mentioned technical scheme, the viscous liquid of texture during the defoaming agent blocks easily in fermentation cylinder feed inlet department, pushes away the defoaming agent propelling movement of valve with fermentation cylinder feed inlet department to the fermentation in through aseptic, improves defoaming efficiency.
In a further embodiment, a scale for weighing the tank is placed on one side of the fermenter.
By adopting the technical scheme, the total amount of the batch addition of the defoaming agent can be measured by a weighing machine.
Has the advantages that: gather height liquid level signal through foam electrode sensor, upload control center's PCL procedure, according to program control solenoid valve on-off time, and then the joining of control defoaming agent, the inside negative compression that produces of hose this moment is flat, and the sealing member makes the connection of hose and solenoid valve be in the state of saturating all the time this moment, prevents that there is the gap at the connection of hose and solenoid valve, prevents that outside bacterium from invading the fermentation cylinder, causes the contamination.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a partial sectional view of the present invention.
The reference signs are: 1. a fermentation tank; 11. a foam electrode sensor; 12. a control center; 2. a storage tank; 21. a hose; 22. an electromagnetic valve; 23. a peristaltic pump; 24. pushing a valve aseptically; 3. a joint; 31. an annular groove; 32. a seal ring; 33. a liner; 34. an annular open slot; 4. a wedge-shaped elastomer; 5. a push block; 6. a threaded sleeve, 7 and a measuring scale.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the present invention.
Referring to fig. 1 and 2, a sealing device of a defoaming system for a cell tank comprises a fermentation tank 1 and a storage tank 2, wherein an output end of the storage tank 2 is communicated with a hose 21, one end of the hose 21, which is far away from the storage tank 2, is communicated with the fermentation tank 1, the hose 21 is sequentially provided with an electromagnetic valve 22 and a power source along the direction of the fermentation tank 1, a sealing element is arranged at a connecting end of the electromagnetic valve 22 and the hose 21, two foam electrode sensors 11 for collecting high and low liquid level signals are arranged at the top of the fermentation tank 1, an output end of the foam electrode sensors 11 is electrically connected with a control center 12, the control center 12 is electrically connected with the electromagnetic valve 22, the high and low liquid level signals are collected by the foam electrode sensors 11 and are uploaded to a PCL program of the control center 12, the electromagnetic valve 22 is controlled to be switched on and off according to the program, so as to further control the addition of a defoaming agent, at the time, negative pressure is generated inside the hose 21 to shrink, at the time, the sealing element makes the connecting end of the hose 21 and the electromagnetic valve 22 always in a full expansion state, the gap between the connecting ends of the hose 21 and the electromagnetic valve 22 is prevented, and external bacteria are prevented from invading the fermentation tank 1 to cause bacteria contamination.
As shown in fig. 2, the sealing element includes a connector 3 screwed on the electromagnetic valve 22, an annular inner liner 33 is disposed on an inner wall of the connector 3, an annular open slot 34 for the hose 21 to be inserted is disposed between the inner liner 33 and an inner wall of the connector 3, a wedge-shaped elastic body 4 and a push block 5 are sleeved on the hose 21, one end of the wedge-shaped elastic body 4 close to the inner liner 33 extends into the annular open slot 34, one end of the wedge-shaped elastic body 4 far away from the annular open slot 34 abuts against the push block 5, a threaded sleeve 6 is screwed on an outer side of the connector 3, one end of the push block 5 far away from the wedge-shaped elastic body 4 abuts against an inner wall of the threaded sleeve 6, the hose 21 is sleeved on an outer side of the inner liner 33, the inner liner 33 abuts against an inner wall of the hose 21 at this time, then the threaded sleeve 6 is rotated, so that the push block 5 pushes the wedge-shaped elastic body 4 to be inserted into the annular open slot 34, the wedge-shaped elastic body 4 clasps the hose 21 on the inner liner 33 at this time, when negative pressure is generated inside the hose 21, the connecting end of the hose 21 and the electromagnetic valve 22 is always in a full expansion state, and the sealing effect is good.
As shown in fig. 2, an annular groove 31 is formed in one side of the liner 33 close to the inner wall of the joint 3, a seal ring 32 is embedded in the annular groove 31, the outer ring of the seal ring 32 abuts against the inner wall of the hose 21, and the seal ring 32 improves the sealing property between the inner wall of the hose 21 and the liner 33 to prevent the leakage of the defoaming agent.
As shown in fig. 1, the power source is a peristaltic pump 23, the hose 21 is clamped at the output end of the peristaltic pump 23, the peristaltic pump 23 alternately extrudes and releases the hose 21, so that the negative pressure inside the hose 21 continuously circulates, and the defoaming agent is pumped into the fermentation tank 1.
As figure 1, aseptic push valve 24 is equipped with at the top of fermentation cylinder 1, aseptic push valve 24 and hose 21 pass through the sealing member switch-on, and aseptic push valve 24 is located the rear end of peristaltic pump 23, and viscous liquid of texture easily blocks at fermentation cylinder 1 feed inlet department during the defoaming agent, pushes away the defoaming agent of fermentation cylinder 1 feed inlet department to the fermentation through aseptic push valve 24, improves defoaming efficiency.
As shown in FIG. 1, a scale 7 for weighing the tank 2 is placed on one side of the fermentation tank 1, and the total amount of the defoaming agent added in a batch can be measured by the scale 7.
The working principle is as follows: the foam electrode sensor 11 collects high and low liquid level signals and uploads the signals to a PCL program of the control center 12, the opening and closing time of the electromagnetic valve 22 is controlled according to a program, at the moment, the peristaltic pump 23 alternately extrudes and releases the hose 21, so that the negative pressure in the hose 21 is continuously circulated, further pumping the defoaming agent into the fermentation tank 1, when the hose 21 is connected, sleeving the hose 21 on the outer side of the liner 33, wherein the inner wall of the hose 21 is abutted by the liner 33, then the screw sleeve 6 is rotated, so that the pushing block 5 pushes the wedge-shaped elastic body 4 to be plugged into the annular open slot 34, at the moment, the wedge-shaped elastic body 4 holds the hose 21 tightly on the inner lining 33, when the hose 21 is internally deflated, the connecting ends of the hose 21 and the electromagnetic valve 22 are always in a full inflation state, the connecting ends of the hose 21 and the electromagnetic valve 22 are prevented from having gaps, and external bacteria are prevented from invading the fermentation tank 1 to cause bacteria contamination.
The preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, however, the present invention is not limited to the details of the above embodiments, and the technical concept of the present invention can be modified to perform various equivalent transformations, which all belong to the protection scope of the present invention.
Claims (6)
1. The utility model provides a sealing device of cell jar defoaming system, includes fermentation cylinder (1) and storage tank (2), its characterized in that, the output end switch-on of storage tank (2) has hose (21), the one end and the fermentation cylinder (1) switch-on of storage tank (2) are kept away from in hose (21), hose (21) are equipped with solenoid valve (22) and power supply in proper order along the direction of fermentation cylinder (1), solenoid valve (22) are equipped with the sealing member with the link of hose (21), fermentation cylinder (1) tank deck is equipped with two foam electrode sensor (11) of gathering high low liquid level signal, the output electric connection of foam electrode sensor (11) has control center (12), control center (12) and solenoid valve (22) electric connection.
2. The sealing device of a cell tank defoaming system according to claim 1, the sealing element comprises a joint (3) which is in threaded connection with the electromagnetic valve (22), the inner wall of the joint (3) is provided with an annular lining (33), an annular open slot (34) for inserting the hose (21) is arranged between the inner liner (33) and the inner wall of the joint (3), the hose (21) is sleeved with a wedge-shaped elastic body (4) and a push block (5), one end of the wedge-shaped elastic body (4) close to the lining (33) extends to the inside of the annular open slot (34), one end of the wedge-shaped elastic body (4) far away from the annular open slot (34) is abutted against the push block (5), the outer side of the joint (3) is in threaded connection with a threaded sleeve (6), and one end, far away from the wedge-shaped elastic body (4), of the push block (5) is abutted to the inner wall of the threaded sleeve (6).
3. The sealing device of the cell tank defoaming system according to claim 2, wherein an annular groove (31) is formed in one side of the lining (33) close to the inner wall of the joint (3), a sealing ring (32) is embedded in the annular groove (31), and the outer ring of the sealing ring (32) abuts against the inner wall of the hose (21).
4. The sealing device of a cell tank defoaming system according to claim 1, wherein the power source is a peristaltic pump (23), and the flexible tube (21) is clamped at the output end of the peristaltic pump (23).
5. The sealing device of the cell tank defoaming system according to claim 4, wherein a sterile push valve (24) is arranged at the top end of the fermentation tank (1), the sterile push valve (24) is communicated with the hose (21) through a sealing member, and the sterile push valve (24) is positioned at the rear end of the peristaltic pump (23).
6. The sealing device of the cell tank defoaming system according to claim 1, characterized in that a scale (7) for weighing the storage tank (2) is placed on one side of the fermentation tank (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220316104.3U CN217377894U (en) | 2022-02-17 | 2022-02-17 | Sealing device of cell tank defoaming system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220316104.3U CN217377894U (en) | 2022-02-17 | 2022-02-17 | Sealing device of cell tank defoaming system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217377894U true CN217377894U (en) | 2022-09-06 |
Family
ID=83100255
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220316104.3U Expired - Fee Related CN217377894U (en) | 2022-02-17 | 2022-02-17 | Sealing device of cell tank defoaming system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217377894U (en) |
-
2022
- 2022-02-17 CN CN202220316104.3U patent/CN217377894U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220906 |
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| CF01 | Termination of patent right due to non-payment of annual fee |